CN112870761A - Preparation process and preparation device of mussel mucin for increasing base material adhesive force - Google Patents

Preparation process and preparation device of mussel mucin for increasing base material adhesive force Download PDF

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Publication number
CN112870761A
CN112870761A CN202110037851.3A CN202110037851A CN112870761A CN 112870761 A CN112870761 A CN 112870761A CN 202110037851 A CN202110037851 A CN 202110037851A CN 112870761 A CN112870761 A CN 112870761A
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China
Prior art keywords
mixing box
mussel
mixing
preparation process
mussel protein
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CN202110037851.3A
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Chinese (zh)
Inventor
王登堂
王宏景
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Shanghai Jianye Technology Co ltd
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Shanghai Jianye Technology Co ltd
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Priority to CN202110037851.3A priority Critical patent/CN112870761A/en
Publication of CN112870761A publication Critical patent/CN112870761A/en
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D11/00Solvent extraction
    • B01D11/02Solvent extraction of solids
    • B01D11/0215Solid material in other stationary receptacles
    • B01D11/0253Fluidised bed of solid materials
    • B01D11/0257Fluidised bed of solid materials using mixing mechanisms, e.g. stirrers, jets
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D11/00Solvent extraction
    • B01D11/02Solvent extraction of solids
    • B01D11/0261Solvent extraction of solids comprising vibrating mechanisms, e.g. mechanical, acoustical
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/145Extraction; Separation; Purification by extraction or solubilisation

Abstract

The invention belongs to the field of mussel protein production, and particularly discloses a preparation process of mussel mucin for increasing substrate adhesive force, which comprises the steps of mixing the mussel protein with protamine, sequentially adding propolis, tea polyphenol and rosemary extract, mixing, adding a proper amount of deionized water, and uniformly mixing to obtain the mussel protein with antiseptic adhesive force; also provides a preparation device, which is used for preparing the mixed material of the mussel protein. The invention combines the mussel protein with natural antiseptic components, not only enhances the antibacterial effect of the mixture, but also has excellent antiseptic performance, and effectively enhances the adhesive force of the mussel protein by utilizing the adhesive performance of the propolis; the inner wall of the whole mixing box of the preparation device is coated with a non-stick coating, and the stirring rods are arranged in a downward inclined mode, so that the materials can flow conveniently and are not easy to adhere to each other; when arranging the material, switch into the form of hanging with the mixing box, under vibrating motor's drive, vibrate the mixing box, the material of being convenient for discharges fast for production efficiency.

Description

Preparation process and preparation device of mussel mucin for increasing base material adhesive force
Technical Field
The invention relates to the field of mussel protein processing, in particular to a preparation process and a preparation device of mussel mucin for increasing substrate adhesive force.
Background
Mussel mucin is extracted from the byssus gland of marine mussel and purified by industrial chromatography to obtain high-purity single protein. Has effects of promoting cell wall crawling, promoting wound healing, inhibiting pruritus, broad-spectrum adhesion, and forming water-resistant protective film. Can be widely used in the fields of surface chemistry, biomedicine, ocean engineering, daily chemical products and the like.
At present, after mussel protein is extracted, the corrosion resistance and the adhesive force of a base material are not ideal enough, so that the storage time and the application effect are influenced; and when mussel protein compounding, the feed liquid adheres to the equipment inner wall easily, and difficult unloading influences the processing progress.
Disclosure of Invention
The invention aims to provide a preparation process and a preparation device of mussel mucin for increasing the adhesive force of a base material, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a preparation process of mussel protein for increasing corrosion-resistant adhesive force comprises the following specific steps:
s1: mixing mussel byssus with an extraction solvent according to the weight ratio of 1: 3-8, leaching at 20-25 deg.C for 15-20min, and crushing frozen Mytilus edulis byssus at high speed with a stirrer to make it uniformly suspended in the extraction solvent;
s2: centrifuging at 10000-;
s3: salting out the obtained supernatant with 1-6 times of 6-12M sodium chloride at 15-25 deg.C for 1-6 hr;
s4: centrifuging at 4 deg.C under 10000-;
s5: centrifuging at 4 deg.C under 10000-;
s6: dialyzing with 0.5-3% acetic acid at 4 deg.C for 3-10 hr to obtain mussel protein, and storing at 4 deg.C;
s7: mixing the mussel protein and protamine, sequentially adding propolis, tea polyphenol and rosemary extract, mixing, adding a proper amount of deionized water, and uniformly mixing to obtain the mussel protein with antiseptic adhesive force.
Preferably, in step S1, the extraction solvent is 0.5-2.5% perchloric acid or/and 1-10% acetic acid.
Preferably, in step S6, the cut-off molecular weight of the dialysis bag used for dialysis is 12000.
Preferably, in step S7, the weight ratio of each component is: mussel protein 3-5 parts, protamine 0.5-2 parts, propolis 1-3 parts, tea polyphenol 0.2-1 parts, and rosemary extract 0.6-1.2 parts.
The invention also provides a preparation device, which is used for preparing and mixing mussel protein and comprises a cross beam and a mixing box, wherein two ends of the cross beam are vertically provided with a bracket, the mixing box is hung below the cross beam through a rope, the center of the top surface of the mixing box is embedded with a bearing, a stirring shaft is arranged in the mixing box, and the stirring shaft is rotatably arranged in the bearing; the beam is provided with a driving motor, and the output end of the driving motor faces downwards and is connected with the upper end of the stirring shaft through a flange plate; the bottom of the mixing box is of a downward arched curved surface structure, and the top end and the bottom end of the mixing box are respectively provided with a feeding port and a discharging port; the support is provided with a vibration motor through a support plate, and the output end of the vibration motor is contacted with the side wall of the mixing box through a vibration ball; the two sides of the bottom of the mixing box are symmetrically provided with positioning sleeves of a hollow structure, the two supports are respectively provided with an electric telescopic rod, and the output ends of the two electric telescopic rods are opposite and are respectively inserted into the corresponding positioning sleeves.
Preferably, the top of the mixing box is symmetrically provided with hanging rings, one end of each rope is fixed on each hanging ring, and the other end of each rope is fixedly connected to the cross beam.
Preferably, a plurality of stirring rods are circumferentially arranged on the stirring shaft from top to bottom, the stirring rods are obliquely arranged downwards, and non-stick coatings are coated on the whole inner wall of the mixing box and the surfaces of the stirring shaft and the stirring rods.
Preferably, the upper end of the stirring shaft extends out of the bearing, the output end of the driving motor and the upper end of the stirring shaft are respectively provided with a flange plate, and the two flange plates are fixedly connected through bolts.
Preferably, the side walls of the mixing box are of double-layer hollow structures, and the cross section between the double-layer side walls is of an arc-shaped curved surface structure with opposite openings.
Preferably, an integrated control panel is arranged on the support and is respectively connected with the control ends of the driving motor, the vibrating motor and the electric telescopic rod.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, protamine, propolis, tea polyphenol and rosemary extract are added into mussel protein and are matched with natural antiseptic components, so that the antibacterial effect of the mixture is enhanced, the mussel protein antiseptic has excellent antiseptic performance, and meanwhile, the adhesive force of the mussel protein is effectively enhanced and the using effect is improved by utilizing the adhesive property of the propolis.
2. The invention provides a preparation device for mussel protein mixing, wherein a mixing box can be switched between two mounting modes of suspension and fixation, non-stick coatings are coated on the whole inner wall of the mixing box, and stirring rods are arranged in a downward inclined mode, so that materials can flow conveniently and are not easy to adhere to the mixing box; when arranging the material, switch into the form of hanging with the mixing box, under vibrating motor's drive, vibrate the mixing box, the material of being convenient for discharges fast for production efficiency.
Drawings
FIG. 1 is a schematic view showing the overall structure of a production apparatus of the present invention;
in the figure: 1. a cross beam; 2. a support; 3. a mixing box; 4. hanging a ring; 5. a rope; 6. a bearing; 7. a stirring shaft; 8. a stirring rod; 9. a drive motor; 10. a flange plate; 11. a bolt; 12. a feed inlet; 13. a discharge port; 14. a support plate; 15. a vibration motor; 16. vibrating the ball; 17. a positioning sleeve; 18. and (5) positioning the sleeve.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "vertical", "upper", "lower", "horizontal", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Example 1: the invention provides a technical scheme that: a preparation process of mussel protein for increasing corrosion-resistant adhesive force comprises the following specific steps:
s1: mixing mussel byssus with an extraction solvent according to the weight ratio of 1: 3-8, leaching at 20-25 deg.C for 15-20min, and crushing frozen Mytilus edulis byssus at high speed with a stirrer to make it uniformly suspended in the extraction solvent;
s2: centrifuging at 10000-;
s3: salting out the obtained supernatant with 1-6 times of 6-12M sodium chloride at 15-25 deg.C for 1-6 hr;
s4: centrifuging at 4 deg.C under 10000-;
s5: centrifuging at 4 deg.C under 10000-;
s6: dialyzing with 0.5-3% acetic acid at 4 deg.C for 3-10 hr to obtain mussel protein, and storing at 4 deg.C;
s7: mixing the mussel protein and protamine, sequentially adding propolis, tea polyphenol and rosemary extract, mixing, adding a proper amount of deionized water, and uniformly mixing to obtain the mussel protein with antiseptic adhesive force.
In this embodiment, in step S1, the extraction solvent is 0.5-2.5% perchloric acid or/and 1-10% acetic acid.
In this example, the cut-off molecular weight of the dialysis bag used for dialysis was 12000 in step S6.
In this embodiment, in step S7, the gravity ratio of each component is: mussel protein 3-5 parts, protamine 0.5-2 parts, propolis 1-3 parts, tea polyphenol 0.2-1 parts, and rosemary extract 0.6-1.2 parts.
Protamine shows strong bacteriostatic ability in neutral and alkaline media, has high thermal stability, still has activity after being heated for 1.5h at 210 ℃, and simultaneously has wide bacteriostatic range and food preservative range. It has strong inhibiting effect on Bacillus subtilis, Bacillus megaterium, Bacillus licheniformis, Bacillus coagulans, Lactobacillus plantarum, Lactobacillus casei, Streptococcus faecalis, etc.
Propolis contains a large amount of active reduction factors, and can be used as natural antioxidant for oil and fat and other food due to its strong oxidation resistance.
The tea polyphenols have good antiseptic and fresh-keeping effects, and can inhibit Bacillus subtilis, Staphylococcus aureus, Escherichia coli, tomato ulcer, Streptococcus saprophyticus, Mucor, Penicillium, Gibberella, anthrax bacteria, and cerevisiae Fermentum.
The main components of rosemary extract are rosmarinol, carnosol and carnosic acid. The main components in the rosemary extract have strong antioxidant activity.
According to the invention, protamine, propolis, tea polyphenol and rosemary extract are added into the mussel protein, so that the mussel protein is matched with natural antiseptic components, the antibacterial effect of the mixture is enhanced, the mussel protein has excellent antiseptic property, and meanwhile, the adhesive force of the mussel protein is effectively enhanced by utilizing the adhesive property of the propolis, and the use effect is improved.
Example 2: referring to fig. 1, the invention also provides a preparation device, which is used for preparing and mixing mussel protein and comprises a beam 1 and a mixing box 3, wherein two ends of the beam 1 are vertically provided with brackets 2, the mixing box 3 is hung below the beam 1 through a rope 5, the center of the top surface of the mixing box 3 is embedded with a bearing 6, a stirring shaft 7 is arranged inside the mixing box 3, and the stirring shaft 7 is rotatably arranged in the bearing 6; a driving motor 9 is arranged on the beam 1, and the output end of the driving motor is downward and is connected with the upper end of the stirring shaft 7 through a flange plate 10; the bottom of the mixing box 3 is of a downward arched curved surface structure, and the top end and the bottom end of the mixing box are respectively provided with a feeding port 12 and a discharging port 13; the support 2 is provided with a vibration motor 15 through a support plate 14, and the output end of the vibration motor is contacted with the side wall of the mixing box 3 through a vibration ball 16; the two sides of the bottom of the mixing box 3 are symmetrically provided with positioning sleeves 17 with hollow structures, the two supports 2 are respectively provided with an electric telescopic rod 18, and the output ends of the two electric telescopic rods 18 are opposite and are respectively inserted into the corresponding positioning sleeves 17.
In this embodiment, the top of the mixing box 3 is symmetrically provided with hanging rings 4, one end of a rope 5 is fixed on the hanging rings 4, and the other end is fixedly connected to the beam 1.
In this embodiment, from top to bottom circumference is provided with many puddlers 8 on the (mixing) shaft 7, and puddler 8 slope sets up downwards, and the whole inner wall of mixing box 3 and (mixing) shaft 7, the 8 surfaces of puddler have all been coated with non-stick coating.
In this embodiment, the upper end of the stirring shaft 7 extends out of the bearing 6, and the output end of the driving motor 9 and the upper end of the stirring shaft 7 are both provided with a flange 10, and the two flanges 10 are fixedly connected by a bolt 11.
In this embodiment, the side walls of the mixing box 3 are of a double-layer hollow structure, and the cross section between the double-layer side walls is of an arc-shaped curved surface structure with opposite openings.
In this embodiment, an integrated control panel is arranged on the support 2, and the integrated control panel is connected with the control ends of the driving motor 9, the vibration motor 15 and the electric telescopic rod 18 respectively.
The working principle is as follows: when the electric equipment works, all the electric equipment is connected to an external power supply.
Fixing the mixing box 3, wherein the output end of a driving motor 9 is fixedly connected with the upper end of a stirring shaft 7 through a flange plate 10, and the output end of an electric telescopic rod 18 is inserted into positioning sleeves 18 at two sides of the mixing box 3 to fix the mixing box 3; sealing the discharge port 13, starting the driving motor 9 to drive the stirring shaft 7 to rotate, adding the mussel protein and the protamine into the mixing box 3 through the feeding port 12, sequentially adding the propolis, the tea polyphenol and the rosemary extract, mixing, adding a proper amount of deionized water, and uniformly mixing; in the process, the stirring rod 8 on the stirring shaft 7 fully stirs the materials.
After the material mixing is finished, the bolts 11 on the flange plate 10 are dismounted, the connection between the driving motor 9 and the stirring shaft 7 is released, and then the output end of the electric telescopic rod 18 is recovered, so that the material mixing box 3 is suspended through the rope 5; then, the discharge port 13 is opened, the vibration motor 15 is started, the mixing box 3 is vibrated, and the vibration amplitude intensity of the mixing box 3 is increased due to the suspension state, so that the blanking is facilitated; the side wall of the mixing box 3 is double-layer hollow, the elasticity is good, and the vibration amplitude is convenient to enhance; the stirring rod 8 is arranged obliquely downwards, the non-stick coating is coated on the whole inner wall of the mixing box 3 and the surfaces of the stirring shaft 7 and the stirring rod 8, the materials are convenient to flow and not easy to adhere to each other, the materials are convenient to discharge quickly, and the production efficiency is accelerated.
It is worth noting that: the whole device realizes control to the control button on the integrated control panel, and because the equipment matched with the control button is common equipment, the device belongs to the prior mature technology, and the electrical connection relation and the specific circuit structure are not repeated.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (9)

1. A preparation process and a preparation device of mussel mucin for increasing base material adhesive force are characterized in that the preparation process comprises the following specific steps:
s1: mixing mussel byssus with an extraction solvent according to the weight ratio of 1: 3-8, leaching at 20-25 deg.C for 15-20min, and crushing frozen Mytilus edulis byssus at high speed with a stirrer to make it uniformly suspended in the extraction solvent;
s2: centrifuging at 10000-;
s3: salting out the obtained supernatant with 1-6 times of 6-12M sodium chloride at 15-25 deg.C for 1-6 hr;
s4: centrifuging at 4 deg.C under 10000-;
s5: centrifuging at 4 deg.C under 10000-;
s6: dialyzing with 0.5-3% acetic acid at 4 deg.C for 3-10 hr to obtain mussel protein, and storing at 4 deg.C;
s7: mixing the mussel protein and protamine, sequentially adding propolis, tea polyphenol and rosemary extract, mixing, adding a proper amount of deionized water, and uniformly mixing to obtain the mussel protein with antiseptic adhesive force;
the preparation device is used for preparing mixed materials in the preparation process and comprises a cross beam (1) and a mixing box (3), wherein supports (2) are vertically arranged at two ends of the cross beam (1), the mixing box (3) is hung below the cross beam (1) through a rope (5), a bearing (6) is embedded in the center of the top surface of the mixing box (3), a stirring shaft (7) is arranged inside the mixing box (3), and the stirring shaft (7) is rotatably arranged in the bearing (6); a driving motor (9) is arranged on the cross beam (1), and the output end of the driving motor is downward and is connected with the upper end of the stirring shaft (7) through a flange plate (10); the bottom of the mixing box (3) is of a curved surface structure which is arched downwards, and the top end and the bottom end of the mixing box are respectively provided with a feeding port (12) and a discharging port (13); a vibration motor (15) is arranged on the support (2) through a support plate (14), and the output end of the vibration motor is contacted with the side wall of the mixing box (3) through a vibration ball (16); the two sides of the bottom of the mixing box (3) are symmetrically provided with positioning sleeves (17) with hollow structures, the two supports (2) are respectively provided with an electric telescopic rod (18), and the output ends of the two electric telescopic rods (18) are opposite and are respectively inserted into the corresponding positioning sleeves (17).
2. The process and apparatus for preparing mussel mucin for increasing substrate adhesion according to claim 1, wherein: in step S1, the extraction solvent is 0.5-2.5% perchloric acid or/and 1-10% acetic acid.
3. The process and apparatus for preparing mussel mucin for increasing substrate adhesion according to claim 1, wherein: in step S6, the cut-off molecular weight of the dialysis bag used for dialysis was 12000.
4. The process and apparatus for preparing mussel mucin for increasing substrate adhesion according to claim 1, wherein: in step S7, the gravity ratio of each component is: mussel protein 3-5 parts, protamine 0.5-2 parts, propolis 1-3 parts, tea polyphenol 0.2-1 parts, and rosemary extract 0.6-1.2 parts.
5. The preparation process and device of mussel mucin for increasing substrate adhesion according to claim 1, wherein: suspension loops (4) are symmetrically arranged at the top of the mixing box (3), one end of a rope (5) is fixed on the suspension loops (4), and the other end of the rope is fixedly connected to the cross beam (1).
6. The preparation process and device of mussel mucin for increasing substrate adhesion according to claim 1, wherein: stirring shaft (7) are gone up from top to bottom circumference and are provided with many puddlers (8), and puddler (8) slope sets up downwards, and mixing box (3) whole inner wall and (mixing) shaft (7), puddler (8) surface all have coated non-stick coating.
7. The preparation process and device of mussel mucin for increasing substrate adhesion according to claim 1, wherein: the upper end of the stirring shaft (7) extends out of the bearing (6), the output end of the driving motor (9) and the upper end of the stirring shaft (7) are both provided with a flange (10), and the two flanges (10) are fixedly connected through a bolt (11).
8. The preparation process and device of mussel mucin for increasing substrate adhesion according to claim 1, wherein: the side wall of the mixing box (3) is of a double-layer hollow structure, and the cross section between the double-layer side walls is of an arc-shaped curved surface structure with opposite openings.
9. The preparation process and device of mussel mucin for increasing substrate adhesion according to claim 1, wherein: the integrated control panel is arranged on the support (2) and is respectively connected with the control ends of the driving motor (9), the vibrating motor (15) and the electric telescopic rod (18).
CN202110037851.3A 2021-01-12 2021-01-12 Preparation process and preparation device of mussel mucin for increasing base material adhesive force Pending CN112870761A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2795103Y (en) * 2005-04-15 2006-07-12 姚革非 Material discharge anti-block device for suspension movable vibration bulk material storehouse
CN101585874A (en) * 2009-06-30 2009-11-25 北京康铭优盛生化技术有限公司 A kind of use saltout and dialyse method of separating and purifying sea-mussel mucin
CN106499183A (en) * 2015-09-07 2017-03-15 为海(泰州)建材有限公司 A kind of concrete hopper
CN108000742A (en) * 2017-12-13 2018-05-08 王耀 A kind of plastic products device for mixing raw material
CN207913855U (en) * 2017-12-29 2018-09-28 天津道谷生物科技有限公司 It is a kind of using fresh and alive earthworm as the forage protein preparation facilities of raw material
CN210206572U (en) * 2019-05-28 2020-03-31 江苏中牧倍康药业有限公司 Mixed feed stirring machine

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2795103Y (en) * 2005-04-15 2006-07-12 姚革非 Material discharge anti-block device for suspension movable vibration bulk material storehouse
CN101585874A (en) * 2009-06-30 2009-11-25 北京康铭优盛生化技术有限公司 A kind of use saltout and dialyse method of separating and purifying sea-mussel mucin
CN106499183A (en) * 2015-09-07 2017-03-15 为海(泰州)建材有限公司 A kind of concrete hopper
CN108000742A (en) * 2017-12-13 2018-05-08 王耀 A kind of plastic products device for mixing raw material
CN207913855U (en) * 2017-12-29 2018-09-28 天津道谷生物科技有限公司 It is a kind of using fresh and alive earthworm as the forage protein preparation facilities of raw material
CN210206572U (en) * 2019-05-28 2020-03-31 江苏中牧倍康药业有限公司 Mixed feed stirring machine

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杜红英: "《绿色食品与有机食品》", 30 June 2019, 武汉大学出版社 *

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Application publication date: 20210601