CN112850914A - Application of rhodium complex in promoting photosynthetic bacteria to degrade azo dye and method for promoting photosynthetic bacteria to degrade azo dye - Google Patents
Application of rhodium complex in promoting photosynthetic bacteria to degrade azo dye and method for promoting photosynthetic bacteria to degrade azo dye Download PDFInfo
- Publication number
- CN112850914A CN112850914A CN202110228859.8A CN202110228859A CN112850914A CN 112850914 A CN112850914 A CN 112850914A CN 202110228859 A CN202110228859 A CN 202110228859A CN 112850914 A CN112850914 A CN 112850914A
- Authority
- CN
- China
- Prior art keywords
- photosynthetic bacteria
- solution
- promoting
- degrade
- rhodium complex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000010948 rhodium Substances 0.000 title claims abstract description 62
- 241000894006 Bacteria Species 0.000 title claims abstract description 55
- 230000000243 photosynthetic effect Effects 0.000 title claims abstract description 53
- 239000000987 azo dye Substances 0.000 title claims abstract description 51
- 229910052703 rhodium Inorganic materials 0.000 title claims abstract description 45
- MHOVAHRLVXNVSD-UHFFFAOYSA-N rhodium atom Chemical compound [Rh] MHOVAHRLVXNVSD-UHFFFAOYSA-N 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 23
- 230000001737 promoting effect Effects 0.000 title claims abstract description 23
- 239000000725 suspension Substances 0.000 claims description 22
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 14
- 241000190967 Rhodospirillum Species 0.000 claims description 14
- 229910052799 carbon Inorganic materials 0.000 claims description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 9
- 239000008103 glucose Substances 0.000 claims description 9
- 238000002835 absorbance Methods 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- CQPFMGBJSMSXLP-UHFFFAOYSA-M acid orange 7 Chemical compound [Na+].OC1=CC=C2C=CC=CC2=C1N=NC1=CC=C(S([O-])(=O)=O)C=C1 CQPFMGBJSMSXLP-UHFFFAOYSA-M 0.000 claims description 3
- JBTHDAVBDKKSRW-UHFFFAOYSA-N chembl1552233 Chemical compound CC1=CC(C)=CC=C1N=NC1=C(O)C=CC2=CC=CC=C12 JBTHDAVBDKKSRW-UHFFFAOYSA-N 0.000 claims description 3
- IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 claims description 3
- STZCRXQWRGQSJD-GEEYTBSJSA-M methyl orange Chemical compound [Na+].C1=CC(N(C)C)=CC=C1\N=N\C1=CC=C(S([O-])(=O)=O)C=C1 STZCRXQWRGQSJD-GEEYTBSJSA-M 0.000 claims description 3
- 229940012189 methyl orange Drugs 0.000 claims description 3
- 229940073450 sudan red Drugs 0.000 claims description 3
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 15
- 230000015556 catabolic process Effects 0.000 abstract description 8
- 238000006731 degradation reaction Methods 0.000 abstract description 8
- 239000002244 precipitate Substances 0.000 abstract description 6
- 238000004042 decolorization Methods 0.000 abstract description 5
- 230000000593 degrading effect Effects 0.000 abstract description 5
- 239000002184 metal Substances 0.000 abstract description 5
- 229910052751 metal Inorganic materials 0.000 abstract description 5
- 229920000642 polymer Polymers 0.000 abstract description 5
- -1 rhodium metal complex Chemical class 0.000 abstract description 4
- 239000003344 environmental pollutant Substances 0.000 abstract 1
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
- 231100000719 pollutant Toxicity 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 55
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 30
- ROFVEXUMMXZLPA-UHFFFAOYSA-N Bipyridyl Chemical compound N1=CC=CC=C1C1=CC=CC=N1 ROFVEXUMMXZLPA-UHFFFAOYSA-N 0.000 description 27
- 239000000975 dye Substances 0.000 description 17
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 9
- 239000002351 wastewater Substances 0.000 description 9
- 239000000126 substance Substances 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 6
- 150000004696 coordination complex Chemical class 0.000 description 6
- WPPDXAHGCGPUPK-UHFFFAOYSA-N red 2 Chemical compound C1=CC=CC=C1C(C1=CC=CC=C11)=C(C=2C=3C4=CC=C5C6=CC=C7C8=C(C=9C=CC=CC=9)C9=CC=CC=C9C(C=9C=CC=CC=9)=C8C8=CC=C(C6=C87)C(C=35)=CC=2)C4=C1C1=CC=CC=C1 WPPDXAHGCGPUPK-UHFFFAOYSA-N 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 239000011259 mixed solution Substances 0.000 description 5
- 238000010170 biological method Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000005515 coenzyme Substances 0.000 description 3
- XWZDJOJCYUSIEY-UHFFFAOYSA-L disodium 5-[(4,6-dichloro-1,3,5-triazin-2-yl)amino]-4-hydroxy-3-phenyldiazenylnaphthalene-2,7-disulfonate Chemical compound [Na+].[Na+].Oc1c(N=Nc2ccccc2)c(cc2cc(cc(Nc3nc(Cl)nc(Cl)n3)c12)S([O-])(=O)=O)S([O-])(=O)=O XWZDJOJCYUSIEY-UHFFFAOYSA-L 0.000 description 3
- 229910052736 halogen Inorganic materials 0.000 description 3
- 150000002367 halogens Chemical class 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- FIKAKWIAUPDISJ-UHFFFAOYSA-L paraquat dichloride Chemical compound [Cl-].[Cl-].C1=C[N+](C)=CC=C1C1=CC=[N+](C)C=C1 FIKAKWIAUPDISJ-UHFFFAOYSA-L 0.000 description 3
- 230000008929 regeneration Effects 0.000 description 3
- 238000011069 regeneration method Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 241000282414 Homo sapiens Species 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 230000001678 irradiating effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N N-phenyl amine Natural products NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- 150000004982 aromatic amines Chemical class 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 125000001624 naphthyl group Chemical group 0.000 description 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
- 125000000449 nitro group Chemical class [O-][N+](*)=O 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 239000010918 textile wastewater Substances 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F15/00—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table
- C07F15/0006—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table compounds of the platinum group
- C07F15/0033—Iridium compounds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F15/00—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table
- C07F15/0006—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table compounds of the platinum group
- C07F15/0073—Rhodium compounds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Environmental & Geological Engineering (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Removal Of Specific Substances (AREA)
Abstract
The invention provides application of a rhodium complex in promoting photosynthetic bacteria to degrade azo dyes and a method for promoting photosynthetic bacteria to degrade azo dyes, belonging to the field of degrading azo dyes by biological bacteria. The method accelerates the degradation rate of the photosynthetic bacteria to the azo dyes by adding an electron medium. Firstly, preparing intermediate precipitate by using rhodium metal polymer, dissolving the intermediate precipitate in water, hydrolyzing the intermediate precipitate to generate rhodium metal complex serving as an electronic medium, taking azo dye as a target pollutant, and adding a proper amount of photosynthetic bacteria into the solution. After the rhodium metal complex is added, the decolorization of the azo dye and the degradation efficiency of COD by the photosynthetic bacteria are higher.
Description
Technical Field
The invention relates to the field of degrading azo dyes by biological bacteria, in particular to application of a rhodium complex in promoting photosynthetic bacteria to degrade azo dyes and a method for promoting photosynthetic bacteria to degrade azo dyes.
Background
China is the largest textile production and export country in the world, and the amount of textile wastewater generated each year accounts for 11% of the total discharge amount of industrial wastewater. The annual dye yield worldwide is about 100 million t, with azo dyes being the most produced and extremely difficult to handle. The high utilization rate of azo dyes leads to the continuous increase of organic wastewater. Azo dyes are characterized by the presence of one or more azo (-N ═ N-) groups, and usually from 1 to 4 groups, linked to phenyl and naphthyl groups. These aromatic groups are usually combined with substituents, such as hydroxy (-OH), methyl (-CH)3) Nitro (-NO)2) Sulfonic acid (-SO)3H) And sodium salt (-SO)3Na), and the like. The azo dye has high chroma, is difficult to degrade in natural environment and has biotoxicity, so the azo dye has serious influence on the ecological balance of the water body. In addition, the azo dye is an aniline organic compound, and can be decomposed to generate more than twenty carcinogenic aromatic amine substances to cause canceration of human bodies under certain special conditions, thereby influencing the health of human beings.
The treatment means of the printing and dyeing wastewater are mainly divided into three major categories, namely a physical method, a chemical method and a biological method, wherein the biological method is the most widely applied method at present. The biological method for treating the dye wastewater refers to that microorganisms take organic pollutants as a carbon source, utilize the self life metabolic activity to separate the dye from the wastewater or convert the dye into an intermediate product or a simple inorganic substance, and further realize sewage purification. However, the dye wastewater has high concentration and complex water quality, and the biological treatment method has certain limitation. Practice shows that the traditional biological method for treating the printing and dyeing wastewater by the wastewater treatment plant cannot effectively degrade organic dyes in the wastewater. However, researches in recent years show that the photosynthetic bacteria have better degradation performance on azo dyes, and the decolorization rate of 12 dyes reaches over 75 percent. Compared with common activated sludge or microbial communities, the photosynthetic bacteria have better adaptability to pH, temperature and salinity, are wider in adaptability and easy to control, can tolerate high-concentration and high-salinity organic wastewater, have certain degradation capability on toxic substances, and cannot generate secondary pollution. Therefore, the photosynthetic bacteria are suitable for degrading azo dye wastewater. As a biological treatment method, the problems of long treatment time, low degradation efficiency and the like still need to be solved.
In the existing multiple coenzyme regeneration technology, Methyl Viologen (MV) is often used2+) And rhodium complex [ CpRh (bpy) (H)2O)]2+As an electronic medium. However, methyl viologen is a toxic substance, easily causes secondary pollution, has strong reducibility and has no selectivity. And [ Cp Rh (bpy) (H)2O)]2+Is the most suitable electronic medium in the enzymatic redox reaction and the regeneration process of various coenzymes at present, can keep activity under a wide range of pH values and temperatures and can maintain stable chemical properties even under extreme conditions, and the prior researches are used for nicotinoyl coenzyme NADH and reduced flavin dinucleotide FADH2And (4) regeneration. But directly adds electronic medium [ Cp Rh (bpy) (H) in complex biological system2O)]2+Studies that promote biological activity are rarely reported.
Disclosure of Invention
The invention aims to provide application of a rhodium complex in promoting photosynthetic bacteria to degrade azo dyes and a method for promoting photosynthetic bacteria to degrade azo dyes.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides an application of a rhodium complex in promoting photosynthetic bacteria to degrade azo dyes, wherein the rhodium complex contains an electron medium [ CpRh (bpy) ((H))2O)]2+。
The invention provides a method for promoting photosynthetic bacteria to degrade azo dyes, which comprises the following steps:
and mixing the photosynthetic bacteria suspension, the organic carbon source, the azo dye solution and the rhodium complex solution.
Further, the photosynthetic bacteria are rhodospirillum; the absorbance value of the photosynthetic bacteria suspension is 1.2-1.6.
Further, the azo dye is one or more of reactive red, methyl orange, congo red, acid orange and Sudan red, and the concentration of the azo dye solution is 45-55 mg/L.
Furthermore, the concentration of the rhodium complex solution is 2.5-3.5 mM.
Further, the organic carbon source is glucose with the concentration of 850-950 mg/L.
Further, the volume ratio of the photosynthetic bacteria suspension to the organic carbon source to the rhodium complex solution to the azo dye solution is 10-15: 450-500: 0.5-2: 200 to 300.
The invention has the beneficial effects that:
the invention adds electron medium [ Cp Rh (bpy) (H) into dye solution containing photosynthetic bacteria2O)]2+The rhodium complex and the rhodium complex are matched with each other, so that the reaction process of the photosynthetic bacteria for degrading the azo dye is accelerated. Compared with the photosynthetic bacteria without the rhodium complex, the photosynthetic bacteria added with the rhodium complex has the dye removal efficiency as high as 82.2 percent and the COD removal efficiency as high as 59 percent.
Drawings
FIG. 1 is a schematic diagram of the effect of the photosynthetic bacteria with added rhodium complex and the photosynthetic bacteria without added rhodium complex on dye decolorization and degradation in example 1.
FIG. 2 is a schematic diagram showing the effect of the photosynthetic bacteria to remove COD in example 1, wherein the rhodium complex is added, and the photosynthetic bacteria are not added with the rhodium complex.
Detailed Description
The invention provides an application of a rhodium complex in promoting photosynthetic bacteria to degrade azo dyes.
In the present invention, the rhodium complex comprises an electron mediator [ CpRh (bpy) (H)2O)]2+。
In the present invention, the rhodium complex is preferably [ Cp × rh (bpy) Cl ] Cl.
The invention provides a method for promoting photosynthetic bacteria to degrade azo dyes, which comprises the following steps:
and mixing the photosynthetic bacteria suspension, the organic carbon source, the azo dye solution and the rhodium complex solution.
In the present invention, the photosynthetic bacteria are preferably rhodospirillum; the absorbance value of the photosynthetic bacteria bacterial suspension is 1.2-1.6, and preferably 1.4.
In the invention, the azo dye is preferably one or more of reactive red, methyl orange, congo red, acid orange and Sudan red, and the reactive red is preferably reactive red 2; the concentration of the azo dye solution is preferably 45-55 mg/L, and more preferably 50 mg/L.
In the invention, the preparation method of the rhodium complex solution comprises the following steps: adding a solvent and 2,2' -bipyridyl into a rhodium metal polymer in sequence to react to obtain a metal complex solution, heating and crystallizing the metal complex solution in a water bath to obtain an intermediate, and mixing the intermediate with water to obtain a rhodium complex solution;
in the present invention, in the above preparation method, the solvent is preferably anhydrous methanol, wherein the volume-to-mass ratio of the solvent, the 2,2' -bipyridine and the rhodium metal polymer is preferably (3 to 5) mL: (9-11) mg: (15-25) mg, more preferably 4 mL: 10.1 mg: 20 mg.
In the present invention, in the above production method, the rhodium metal polymer is preferably [ Cp + RhCl ]2]2。
In the preparation method, the reaction temperature of the 2,2' -bipyridyl and the rhodium metal polymer is 20-30 ℃, and preferably 25 ℃.
In the present invention, in the above preparation method, the temperature for heating the metal complex solution in a water bath is 55 to 65 ℃, preferably 60 ℃; the heating time is 1.5-2.5h, preferably 2 h.
In the present invention, the concentration of the rhodium complex solution is preferably 2.5 to 3.5mM, and more preferably 3.0 mM.
In the invention, the organic carbon source is preferably glucose with the concentration of 850-950 mg/L, more preferably glucose with the concentration of 880-920 mg/L, and even more preferably glucose with the concentration of 900 mg/L.
In the invention, the volume ratio of the photosynthetic bacteria suspension, the organic carbon source, the rhodium complex solution and the azo dye solution is preferably 10-15: 450-500: 0.5-2: 200 to 300, more preferably 11 to 14: 470-480: 1-1.8: 220 to 280, more preferably 13: 475: 1.5: 250.
the technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
1. 20.00mg of [ Cp + RhCl ] was taken2]2Placing into a beaker, adding 4mL of anhydrous methanol, red [ Cp + RhCl2]2Insoluble and suspended in methanol. To the suspension was added 10.10mg of 2,2' -bipyridine and the mixture was reacted with [ Cp + RhCl ] at 25 deg.C2]2The reaction lasts for 2h to form a metal complex, and the red suspended substance gradually disappears to generate an orange solution. The solution was heated in a water bath at 55 ℃ for 2h to evaporate the methanol and the solution was concentrated to 1 mL. At 4 deg.C, dropwise adding anhydrous diethyl ether to the concentrated solution to make [ Cp Rh (bpy) Cl]Cl gradually precipitated. The precipitate was collected and dissolved in water to prepare a 3mM solution, which was stored at 4 ℃. [ Cp + Rh (bpy) Cl]Cl is immediately hydrolyzed in water to generate [ Cp Rh (bpy) ((H))2O)]2+。
2. Taking 2 erlenmeyer flasks, and marking as an erlenmeyer flask 1 and an erlenmeyer flask 2;
the conical flask 1: 250mL of active red 2 solution with the concentration of 50mg/L, bacterial suspension prepared by rhodospirillum with the wet weight of 3g and 0.225g of glucose; the absorbance of the rhodospirillum suspension measured at OD660 was 1.2.
The conical flask 2: 250mLActivated Red 2 solution with concentration of 50mg/L, suspension prepared from Rhodospirillum with wet weight of 3g, glucose 0.225g, and 1mL of prepared suspension containing [ Cp Rh (bpy) ((H))2O)]2+The solution of (1);
after the mixture is uniformly mixed, the mixed solution in the two conical flasks is irradiated for 12 hours under a halogen lamp light source of 300W, then sampling is respectively carried out, and the concentration of the azo dye and the removal rate of COD in the two conical flasks are calculated, wherein the sampling time interval is 24 hours.
Example 1 of the invention will determine whether to add [ Cp Rh (bpy) (H)2O)]2+As experimental variables, the effect of adding or not adding rhodium complex on the degradation of dye by photosynthetic bacteria was compared. The sampling time interval was 24 hours, and the decolorization of the dye and the removal of COD were analyzed. As can be seen from FIG. 1, the rate of decolorization is much faster after the addition of the rhodium complex to the dye solution than when the rhodium complex solution is not added. As can be seen from fig. 2, the removal rate of COD in the dye solution by the photosynthetic bacteria was always higher than that of the solution without the rhodium complex added. From this, it was found that the addition of rhodium complex in the dye solution can promote the degradation of the photosynthetic bacteria.
Example 2
1. 25.00mg of [ Cp + RhCl ] was taken2]2Placing into a beaker, adding 3mL of anhydrous methanol, red [ Cp + RhCl2]2Insoluble and suspended in methanol. To the suspension was added 15.00mg of 2,2' -bipyridine and the mixture was reacted with [ Cp + RhCl ] at 30 ℃2]2The reaction is carried out for 1.5h to form a metal complex, and the red suspended substance gradually disappears to generate an orange solution. The solution was heated in a water bath at 60 ℃ for 1.5h to evaporate the methanol and the solution was concentrated to 1 mL. At 4 deg.C, dropwise adding anhydrous diethyl ether to the concentrated solution to make [ Cp Rh (bpy) Cl]Cl gradually precipitated. The precipitate was collected and dissolved in water to prepare a 4mM solution and stored at 4 ℃. [ Cp + Rh (bpy) Cl]Cl is immediately hydrolyzed in water to generate [ Cp Rh (bpy) ((H))2O)]2+。
2. Taking a conical flask, and preparing 250mL of active red 2 solution with the concentration of 50 mg/L;
and (3) preparing rhodospirillum with wet weight of 3g into bacterial suspension, and adding the rhodospirillum bacterial suspension into the prepared active red 2 solution for mixing. The absorbance of the rhodospirillum suspension measured at OD660 was 1.4.
3. 0.225g of glucose is used as an organic carbon source required by growth of rhodospirillum, and the initial carbon source concentration is 900mg/L when the solution prepared in the step 2 is added.
4. 1.5mL of the prepared solution containing [ Cp Rh (bpy) (H)2O)]2+And (3) adding the solution in the step (2) into the mixed solution, uniformly stirring, and irradiating the mixed solution for 12 hours under a 300W halogen lamp light source.
By verification, the treatment method obtained in example 2 has the removal rate of 88% for the reactive red 2 dye solution and the removal rate of COD of 55%.
Example 3
1. 20.00mg of [ Cp + RhCl ] was taken2]2Placing into a beaker, adding 5mL of anhydrous methanol, red [ Cp + RhCl2]2Insoluble and suspended in methanol. To the suspension was added 10.10mg of 2,2' -bipyridine and the mixture was reacted with [ Cp + RhCl ] at 20 deg.C2]2The reaction lasts for 2h to form a metal complex, and the red suspended substance gradually disappears to generate an orange solution. The solution was heated in a water bath at 65 ℃ for 2h to evaporate the methanol and the solution was concentrated to 1 mL. At 4 deg.C, dropwise adding anhydrous diethyl ether to the concentrated solution to make [ Cp Rh (bpy) Cl]Cl gradually precipitated. The precipitate was collected and dissolved in water to prepare a 4mM solution and stored at 4 ℃. [ Cp + Rh (bpy) Cl]Cl is immediately hydrolyzed in water to generate [ Cp Rh (bpy) ((H))2O)]2+。
2. Taking a conical flask, and preparing 250mL of active red 2 solution with the concentration of 50 mg/L;
and (3) preparing rhodospirillum with wet weight of 3g into bacterial suspension, and adding the rhodospirillum bacterial suspension into the prepared active red 2 solution for mixing. The absorbance of the rhodospirillum suspension measured at OD660 was 1.6.
3. 0.225g of glucose is used as an organic carbon source required by growth of rhodospirillum, and the initial carbon source concentration is 900mg/L when the solution prepared in the step 2 is added.
4. 0.8mL of the prepared solution containing [ Cp Rh (bpy) (H)2O)]2+And (3) adding the solution in the step (2) into the mixed solution, uniformly stirring, and irradiating the mixed solution for 12 hours under a 300W halogen lamp light source.
By verification, the treatment method obtained in example 2 has the removal rate of 86.7% for the reactive red 2 dye solution and the removal rate of COD of 56%.
From the above embodiments, the present invention provides an application of rhodium complex in promoting photosynthetic bacteria to degrade azo dyes and a method for promoting photosynthetic bacteria to degrade azo dyes. The invention relates to an electron mediator [ Cp + Rh (bpy) (H) with excellent enzyme activity2O)]2+The method is added into a biological bacteria system, greatly accelerates the speed of the photosynthetic bacteria degrading azo dyes and the removal rate of COD, and is safe, environment-friendly, simple to operate and high in feasibility.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (7)
1. The application of rhodium complex in promoting photosynthetic bacteria to degrade azo dye is characterized in that the rhodium complex contains electron medium [ CpRh (bpy) ((H))2O)]2+。
2. A method for promoting photosynthetic bacteria to degrade azo dyes is characterized by comprising the following steps:
and mixing the photosynthetic bacteria suspension, the organic carbon source, the azo dye solution and the rhodium complex solution.
3. A method for promoting photosynthetic bacteria to degrade azo dyes according to claim 2 wherein the photosynthetic bacteria are rhodospirillum; the absorbance value of the photosynthetic bacteria suspension is 1.2-1.6.
4. The method for promoting photosynthetic bacteria to degrade azo dyes according to claim 3, wherein the azo dyes are one or more of reactive red, methyl orange, congo red, acid orange and Sudan red, and the concentration of the azo dye solution is 45-55 mg/L.
5. The method for promoting photosynthetic bacteria to degrade azo dyes of claim 4 wherein the concentration of the rhodium complex solution is 2.5-3.5 mM.
6. The method for promoting photosynthetic bacteria to degrade azo dyes of claim 5, wherein the organic carbon source is glucose with a concentration of 850-950 mg/L.
7. The method for promoting photosynthetic bacteria to degrade azo dyes according to any one of claims 2 to 6, wherein the volume ratio of the photosynthetic bacteria suspension, the organic carbon source, the rhodium complex solution and the azo dye solution is 10-15: 450-500: 0.5-2: 200 to 300.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110228859.8A CN112850914B (en) | 2021-03-02 | 2021-03-02 | Application of rhodium complex in promoting photosynthetic bacteria to degrade azo dye and method for promoting photosynthetic bacteria to degrade azo dye |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110228859.8A CN112850914B (en) | 2021-03-02 | 2021-03-02 | Application of rhodium complex in promoting photosynthetic bacteria to degrade azo dye and method for promoting photosynthetic bacteria to degrade azo dye |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112850914A true CN112850914A (en) | 2021-05-28 |
| CN112850914B CN112850914B (en) | 2022-04-05 |
Family
ID=75990899
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110228859.8A Active CN112850914B (en) | 2021-03-02 | 2021-03-02 | Application of rhodium complex in promoting photosynthetic bacteria to degrade azo dye and method for promoting photosynthetic bacteria to degrade azo dye |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112850914B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10339790A1 (en) * | 2003-08-28 | 2005-03-17 | Umicore Ag & Co.Kg | Rhodium (I) diene bis-aqua complexes, process for their preparation and their use |
| CN101050444A (en) * | 2007-03-09 | 2007-10-10 | 重庆大学 | Bacterial strain of photosynthetic bacterium possessing behavior of producing hydrogen |
| CN101948768A (en) * | 2010-08-20 | 2011-01-19 | 沈阳大学 | Method for preparing photosynthetic bacteria for degrading 2-chlorophenol |
-
2021
- 2021-03-02 CN CN202110228859.8A patent/CN112850914B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10339790A1 (en) * | 2003-08-28 | 2005-03-17 | Umicore Ag & Co.Kg | Rhodium (I) diene bis-aqua complexes, process for their preparation and their use |
| CN101050444A (en) * | 2007-03-09 | 2007-10-10 | 重庆大学 | Bacterial strain of photosynthetic bacterium possessing behavior of producing hydrogen |
| CN101948768A (en) * | 2010-08-20 | 2011-01-19 | 沈阳大学 | Method for preparing photosynthetic bacteria for degrading 2-chlorophenol |
Non-Patent Citations (1)
| Title |
|---|
| 周汝利: "石墨烯改性TiO2光催化再生辅酶及[Cp*Rh(bpy)(H2O)]2+前体的固定化", 《中国优秀硕士学位论文全文数据库 工程科技Ｉ辑》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112850914B (en) | 2022-04-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Shi et al. | Pretreatment of saline antibiotic wastewater using marine microalga | |
| CN106698658B (en) | Method for quickly forming anaerobic ammonium oxidation granular sludge | |
| Maniyam et al. | Decolourization and biodegradation of azo dye methyl red by Rhodococcus strain UCC 0016 | |
| CN101538097B (en) | Enzyme treatment technique of difficultly degradable organic sewage | |
| Yang et al. | Denitrification of aging biogas slurry from livestock farm by photosynthetic bacteria | |
| CN106434469B (en) | Low-temperature-resistant nitrifying bacteria agent and preparation method and application thereof | |
| CN104150608A (en) | Biochemical treatment process for high salinity wastewater | |
| Peng et al. | Microbiology community changes during the start-up and operation of a photosynthetic bacteria-membrane bioreactor for wastewater treatment | |
| CN114480225B (en) | Method for biologically and intensively treating high-salt chemical wastewater | |
| CN113307377A (en) | Method for treating fermentation exhaust gas and wastewater by coupling active microalgae | |
| CN110656133B (en) | Pretreatment method for promoting production of medium-chain fatty acid by anaerobic fermentation of waste activated sludge | |
| CN103387289A (en) | Method for strengthening azo dye biodegradation by utilizing zero-valent iron | |
| Yang et al. | A special light-aerobic condition for photosynthetic bacteria-membrane bioreactor technology | |
| CN112850914B (en) | Application of rhodium complex in promoting photosynthetic bacteria to degrade azo dye and method for promoting photosynthetic bacteria to degrade azo dye | |
| Pal et al. | Wheat straw-based microbial electrochemical reactor for azo dye decolorization and simultaneous bioenergy generation | |
| CN112794577A (en) | Remove SO42-And Cr (VI) | |
| US12060291B2 (en) | Method for treatment and resource utilization of acidic organic wastewater | |
| CN102994565A (en) | Method for enhancing anaerobic fermentation of algal waste liquid to generate methane | |
| CN107265809B (en) | Method for degrading polycyclic aromatic hydrocarbon in sludge through anaerobic co-metabolism | |
| CN114477452A (en) | Method for removing tetracycline antibiotics in landfill leachate | |
| CN112028251A (en) | Method for improving ammonia nitrogen removal effect of microalgae on livestock and poultry breeding wastewater | |
| Dong et al. | Degradation of isatin and anthranilic acid by Achromobacter xylosoxidans N4 isolated from denim-manufacturing mill sludge | |
| CN113322196B (en) | Bacillus methylotrophicus for producing single-cell protein by utilizing aromatic nitro compound | |
| CN117089543B (en) | Water treatment microbial inoculum and application thereof in aquaculture wastewater treatment | |
| CN113594524B (en) | Microbial electrosynthesis reactor for supplying carbon dioxide by using hollow fiber membrane and using method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zhang Xinying Inventor after: Lin Muxue Inventor after: Liu Lu Inventor after: Wang Yongjing Inventor after: Liu Minghua Inventor after: Zhao Shaohong Inventor after: Huang Yanqi Inventor after: Zhang Yucheng Inventor after: Zhou Ya Inventor before: Zhang Xinying Inventor before: Lin Muxue Inventor before: Wang Yongjing Inventor before: Liu Minghua Inventor before: Liu Lu Inventor before: Zhao Shaohong Inventor before: Huang Yanqi Inventor before: Zhang Yucheng Inventor before: Zhou Ya |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |