CN112834492A - Detection reagent and kit for rapidly detecting potassium bromate - Google Patents
Detection reagent and kit for rapidly detecting potassium bromate Download PDFInfo
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- CN112834492A CN112834492A CN202011640239.7A CN202011640239A CN112834492A CN 112834492 A CN112834492 A CN 112834492A CN 202011640239 A CN202011640239 A CN 202011640239A CN 112834492 A CN112834492 A CN 112834492A
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- 238000001514 detection method Methods 0.000 title claims abstract description 81
- XWNSFEAWWGGSKJ-UHFFFAOYSA-N 4-acetyl-4-methylheptanedinitrile Chemical compound N#CCCC(C)(C(=O)C)CCC#N XWNSFEAWWGGSKJ-UHFFFAOYSA-N 0.000 title claims abstract description 70
- 239000004153 Potassium bromate Substances 0.000 title claims abstract description 70
- 235000019396 potassium bromate Nutrition 0.000 title claims abstract description 70
- 229940094037 potassium bromate Drugs 0.000 title claims abstract description 70
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 69
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims abstract description 63
- 238000012360 testing method Methods 0.000 claims abstract description 15
- 239000011111 cardboard Substances 0.000 claims abstract description 11
- 150000007522 mineralic acids Chemical class 0.000 claims abstract description 9
- 235000013305 food Nutrition 0.000 claims abstract description 8
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 30
- 235000013312 flour Nutrition 0.000 claims description 25
- 239000000758 substrate Substances 0.000 claims description 21
- 235000008429 bread Nutrition 0.000 claims description 9
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 6
- 241000209140 Triticum Species 0.000 claims description 6
- 235000021307 Triticum Nutrition 0.000 claims description 6
- 229960000583 acetic acid Drugs 0.000 claims description 3
- 239000012362 glacial acetic acid Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 9
- 238000011161 development Methods 0.000 abstract description 6
- 239000000243 solution Substances 0.000 description 26
- 239000008367 deionised water Substances 0.000 description 12
- 229910021641 deionized water Inorganic materials 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 239000000203 mixture Substances 0.000 description 10
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- 238000004458 analytical method Methods 0.000 description 4
- 239000003086 colorant Substances 0.000 description 4
- 239000012086 standard solution Substances 0.000 description 4
- 239000000123 paper Substances 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 108010068370 Glutens Proteins 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 239000007844 bleaching agent Substances 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000021312 gluten Nutrition 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 210000005036 nerve Anatomy 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 235000010037 flour treatment agent Nutrition 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000003918 potentiometric titration Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/10—Starch-containing substances, e.g. dough
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/775—Indicator and selective membrane
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Food Science & Technology (AREA)
- Plasma & Fusion (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention provides a detection reagent and a kit for rapidly detecting potassium bromate, wherein the detection reagent is used for rapidly detecting the potassium bromate in food and comprises the following components: the detection reagent A is 0.5-1.0mol/L inorganic acid, and the detection reagent B is 1.8-2.3mol/L potassium iodide solution. When the pH value of the reagent is 1-3, the reagent has the advantages of short color development time, stable color, high detectable rate and no need of avoiding light in the color development process. The rapid detection requirement can be met. The kit for rapidly detecting potassium bromate comprises: detection reagent A, detection reagent B, centrifuging tube, color comparison cardboard and pipette etc.. When the detection is needed, the sample to be detected is firstly processed, the detection reagent A and the detection reagent B are added into the processed sample, the processed sample is mixed and then stands still, then the color is compared with the color level on the color comparison card, and whether the sample to be detected contains the potassium bromate or not is obtained according to the comparison result. Through comparison tests, the detection limit of the detection kit provided by the invention is 5 mg/kg.
Description
Technical Field
The invention relates to the technical field of food detection, in particular to a detection reagent and a kit for rapidly detecting potassium bromate.
Background
Potassium bromate is an inorganic salt, is a colorless crystal at room temperature, and is mainly used as an analytical reagent, an oxidizing agent, a wool bleaching treatment agent, a food additive (which is now banned in china), and the like. The potassium bromate can bleach flour due to its oxidability, inhibit activity of protease, improve strength and elasticity of gluten, and has low cost, and can be used in baking industry for a long time. However, excessive consumption of potassium bromate can damage the central nerve, blood and kidney of people, the compound is also classified as a carcinogenic substance by international cancer research institutions, the use of potassium bromate as a flour treating agent in wheat flour is prohibited by the instruction of China in No. 9 publication of 2005, and flour manufacturers still add potassium bromate to improve the quality of the flour.
At present, methods for measuring potassium bromate mainly comprise a potentiometric titration method, a sulfite titration method, a liquid chromatography method, an ion chromatography method and the like, wherein the chromatography method and the like need large-scale instruments and equipment, the analysis cost is too high, professional personnel are needed for detection, the detection time is too long, and the detection result is not output timely.
Therefore, how to rapidly detect whether the food contains potassium bromate is an urgent problem to be solved.
Disclosure of Invention
The invention provides a reagent and a kit for rapidly detecting potassium bromate, and aims to solve the technical problems that in the prior art, the detection of potassium bromate is high in analysis cost and needs to depend on professional equipment to a certain extent.
The technical scheme for solving the technical problems is as follows:
a reagent for rapidly detecting potassium bromate, which is used for rapidly detecting the potassium bromate in food, and comprises the following components: the detection reagent A is 0.5-1.0mol/L inorganic acid, and the detection reagent B is 1.8-2.3mol/L potassium iodide solution.
Optionally, the detection reagent for rapidly detecting potassium bromate, wherein the inorganic acid comprises: hydrochloric acid, sulfuric acid and glacial acetic acid.
Optionally, the detection reagent for rapidly detecting potassium bromate is provided, wherein the detection limit of the detection reagent is 5 mg/kg.
A kit for rapidly detecting potassium bromate comprises the detection reagent.
Optionally, the kit, wherein the kit further comprises: box body, color comparison cardboard and centrifuging tube.
Optionally, the kit of (a), wherein the colorimetric card: the kit comprises a substrate and a standard colorimetric color scale fixed on the surface of the substrate and used for detecting potassium bromate.
Optionally, in the kit, a groove for accommodating a detection tube is provided between two adjacent color levels.
Optionally, the kit, wherein the color gradation comprises: yellow, magenta, purple, and bluish-purple.
Optionally, the kit, wherein the colorimetric card comprises: the colorimetric card board I is used for detecting potassium bromate in the wheat flour and the colorimetric card board II is used for detecting potassium bromate in the bread.
Optionally, the kit, wherein the detection reagent B is contained in a black reagent bottle.
Has the advantages that: the invention provides a detection reagent and a kit for rapidly detecting potassium bromate, wherein the detection reagent comprises a detection reagent A and a detection reagent B, the detection reagent A is 0.5-1.0mol/L inorganic acid, and the detection reagent B is 1.8-2.3mol/L potassium iodide solution. When the pH value of the reagent is 1-3, the reagent has the advantages of short color development time, stable color, high detectable rate and no need of avoiding light in the color development process. The rapid detection requirement can be met.
Drawings
FIG. 1 is a schematic diagram of the appearance of a kit for rapid detection of potassium bromate according to an embodiment of the present invention;
fig. 2 is a schematic diagram of the kit for rapidly detecting potassium bromate provided by the embodiment of the present invention after being opened.
Detailed Description
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
The potassium bromate can bleach flour due to its oxidability, inhibit activity of protease, improve strength and elasticity of gluten, and has low cost, and can be used in baking industry for a long time. However, excessive consumption of potassium bromate can damage the central nerve, blood and kidney of people, and although the national command prohibits the use of potassium bromate as a flour treatment agent in wheat flour, illegal people can add potassium bromate into the flour.
Therefore, flour and food taking flour as raw materials need to be monitored and detected, but the existing detection of potassium bromate in flour and food taking flour as raw materials usually needs large instruments and equipment such as a chromatograph and a mass spectrometer, so that the analysis cost is high, the specialty is strong, and the requirement of rapid detection cannot be met.
Illustratively, the wheat flour sold in a farmer market is subjected to spot inspection, if according to the existing detection method, an inspector needs to collect a sample on site, the sample is packaged and taken back to a laboratory for detection by a large-scale analytical instrument, the whole detection process takes a long time from sampling to outputting of an analysis result, and the detection result is very likely to appear, shows that the batch of flour contains potassium bromate, but because the detection result cannot be obtained in the first time, the batch of flour is sold to consumers by a merchant. Leading to problematic flours moving to the market.
Based on this, the present invention provides a solution to the above technical problem, and the details thereof will be explained in the following embodiments.
As shown in fig. 1 to fig. 2, an embodiment of the present invention provides a kit for rapidly detecting potassium bromate, including: the kit body 10, the reagent bottle 11 that inside was equipped with detect reagent A, the reagent bottle 12 that inside was equipped with detect reagent B, color comparison cardboard 13, color comparison cardboard 14, centrifuging tube 15 and centrifuging tube 16.
In this embodiment, the box 10 is square, and the material thereof may be coated paper or plastic, the detection reagent a is 0.5-1.0mol/L of inorganic acid, and the detection reagent B is 1.8-2.3mol/L of potassium iodide solution. Wherein, the inorganic acid includes but is not limited to hydrochloric acid, sulfuric acid, glacial acetic acid. The packing specifications of the reagent bottles 11 and 12 can be as follows: 25 mL/bottle, 20 mL/bottle, 15 mL/bottle. The centrifuge tube 15 is a centrifuge tube with a specification of 15ml, and the centrifuge tube 16 is a centrifuge tube with a specification of 2 ml.
In this embodiment, the reagent bottle 11 is a brown reagent bottle or a white reagent bottle, and the reagent bottle 12 is a black reagent bottle. The detection reagent B is placed in a black reagent bottle, so that the influence of the reaction of potassium iodide on normal use under the illumination condition can be avoided.
In this embodiment, the concentration of the substance of the detection reagent B is controlled to be 1.8-2.3mol/L, which can avoid that the concentration of potassium iodide is too high, and excessive iodine simple substance is generated in the detection (reaction) process, which causes errors in detection of negative samples and false positives. Meanwhile, the detection result of a positive sample is wrong and false negative caused by low potassium iodide concentration can be avoided. In other words, the occurrence of false negative or false positive can be avoided by controlling the mass concentration of the detecting reagent B to 1.8 to 2.3 mol/L.
In one implementation of this embodiment, the colorimetric card comprises a substrate (base) and a colorimetric scale for standard colorimetric detection of potassium bromate, wherein the substrate (base) is fixed on the surface of the substrate. The fixing can be realized by adhering the color scale on the surface of the substrate by using an adhesive manner, or fixing the color scale on the surface of the substrate by using a plastic package manner. The substrate can be a plastic substrate, and the color of the substrate is white. The size of the substrate can be set according to actual needs, and the specific product size is not required here.
In an implementation manner of this embodiment, the color level may also be fixed by printing, in other words, the color level is printed on a paper sheet, such as a coated paper sheet, by color printing.
In this embodiment, the color scale of the standard colorimetry for detecting potassium bromate refers to the color of potassium bromate solutions with different concentrations after detection reagent a and detection reagent B are added. The colors shown include yellow, magenta, purple or bluish-purple, etc. The different colors are fixed on the surface of the substrate in sequence according to the different colors, and the series of different colors form a color level.
In this embodiment, the colorimetric cards comprise a colorimetric card I for detecting potassium bromate in wheat flour and a colorimetric card II for detecting potassium bromate in bread. It should be noted that the colorimetric card I corresponds to the colorimetric card 13, and the colorimetric card II corresponds to the colorimetric card 14.
Illustratively, the standard colorimetric color scale in the colorimetric card I is prepared as follows:
weighing 1g of flour without potassium bromate, adding 0.0 muL, 5.0 muL, 10.0 muL, 20.0 muL, 30.0 muL, 40.0 muL and 50.0 muL of 1000mg/L potassium bromate standard solution respectively, adding 5mL of deionized water, shaking and uniformly mixing, standing for 5 minutes, taking 0.5mL of supernatant from each centrifuge tube into 2mL centrifuge tubes respectively, adding 1 drop of 0.5-1.0mol/L hydrochloric acid and 2 drops of 1.8-2.3mol/L potassium iodide solution respectively, shaking and uniformly mixing, soaking the cut test strip in the test strip, standing for 1 minute at room temperature, taking out the test strip, drying in the air, and fixing the dried test strip on a substrate according to a certain sequence to obtain the colorimetric card I.
Further, as an improved scheme, a plurality of 15mL centrifuge tubes are taken, 1g of flour without potassium bromate is weighed, 0.0 μ L, 5.0 μ L, 10.0 μ L, 20.0 μ L, 30.0 μ L, 40.0 μ L and 50.0 μ L of 1000mg/L potassium bromate standard solution are respectively added, 5mL of deionized water is added, the mixture is shaken and uniformly mixed and then stands for 5 minutes, 0.5mL of supernatant is respectively taken from each centrifuge tube to 2mL centrifuge tubes, 1 drop of 0.5-1.0mol/L hydrochloric acid and 2 drops of 1.8-2.3mol/L potassium iodide solution are respectively added, after the mixture is shaken and uniformly mixed, a color card is manufactured on a computer according to the color displayed by the solution in 1 minute, and the color card I is printed out.
Illustratively, the standard colorimetric color scale in the colorimetric card II is prepared as follows:
weighing 1g of bread without potassium bromate and having the skin torn off, adding 0.0 muL, 5.0 muL, 10.0 muL, 20.0 muL, 30.0 muL, 40.0 muL and 50.0 muL of 1000mg/L potassium bromate standard solution respectively, adding 7mL of deionized water, shaking and uniformly mixing, standing for 5 minutes, putting 0.5mL of soak solution into 2mL of a centrifuge tube, adding 1 drop of 0.5-1.0mol/L hydrochloric acid and 1 drop of 1.8-2.3mol/L potassium iodide solution, shaking and uniformly mixing, soaking the cut test strip in the test strip, taking out the test strip, drying the test strip in the air, and fixing the dried test strip on a substrate according to a certain sequence to obtain the colorimetric card plate II.
Further, as an improved scheme, a plurality of 15mL centrifuge tubes are taken, 1g of bread without potassium bromate and with the peel removed is weighed, 0.0 μ L, 5.0 μ L, 10.0 μ L, 20.0 μ L, 30.0 μ L, 40.0 μ L and 50.0 μ L of 1000mg/L potassium bromate standard solution is respectively added, 7mL of deionized water is added, the mixture is shaken and mixed uniformly and then stands for 5 minutes, 0.5mL of soak solution is taken to be added into 2mL centrifuge tubes, 1 drop of 0.5-1.0mol/L hydrochloric acid and 1 drop of 1.8-2.3mol/L potassium iodide solution are added, the mixture is shaken and mixed uniformly and then develops color according to the solution, a color card is made on a computer and printed, and the color card II is obtained.
In one implementation of this embodiment, a groove for accommodating the centrifuge tube is provided between two adjacent color levels.
Specifically, an arc-shaped groove 140 is formed in the substrate, and the arc-shaped groove is arranged according to the color level direction of the standard colorimetric for detecting potassium bromate, for example, the fixing direction of the color level of the standard colorimetric for detecting potassium bromate is transversely arranged, and then the arc-shaped groove is also arranged according to the arc-shaped arrangement. The length of the arc-shaped groove can penetrate through the substrate, namely the arc-shaped groove is the same as the width of the substrate, and of course, the length of the arc-shaped groove can be smaller than the width of the substrate. Note that one end (open end) of the arc-shaped groove is flush with one end of the substrate. Through setting up the arc slot on the base plate, at the fixed colour gradation in the both sides of arc slot, can make things convenient for the color comparison, promptly, when needs carry out the color comparison, will treat that the detection test tube of color comparison inserts arc slot is interior, fixes it on the base plate, can carry out the colour and compare. The test tube can be operated by one hand without using two hands (one hand holds the test tube and the other hand holds the colorimetric plate).
The use of the kit for rapid detection of potassium bromate provided by the present invention is further explained by the following specific examples.
Example 1
And (3) putting 1g of flour into a 15mL centrifuge tube, adding 5mL of deionized water, shaking, uniformly mixing, standing for 5 minutes, putting 0.5mL of supernatant into a 2mL centrifuge tube, and adding 1 drop of 0.5mol/L hydrochloric acid and 2 drops of 1.8mol/L potassium iodide solution. After shaking and mixing, the mixture is placed for 1 minute at room temperature and then is compared with a standard color card I for detecting potassium bromate to roughly quantify.
Example 2
And (3) putting 1g of flour into a 15mL centrifuge tube, adding 5mL of deionized water, shaking, uniformly mixing, standing for 5 minutes, putting 0.5mL of supernatant into a 2mL centrifuge tube, and adding 1 drop of 0.5mol/L hydrochloric acid and 2 drops of 2.3mol/L potassium iodide solution. After shaking and mixing, the mixture is placed for 1 minute at room temperature and then is compared with a standard color card I for detecting potassium bromate to roughly quantify.
Example 3
And (3) putting 1g of flour into a 15mL centrifuge tube, adding 5mL of deionized water, shaking, uniformly mixing, standing for 5 minutes, putting 0.5mL of supernatant into a 2mL centrifuge tube, and adding 1 drop of 1.0mol/L hydrochloric acid and 2 drops of 1.8mol/L potassium iodide solution. After shaking and mixing, the mixture is placed for 1 minute at room temperature and then is compared with a standard color card I for detecting potassium bromate to roughly quantify.
Example 4
And (3) putting 1g of flour into a 15mL centrifuge tube, adding 5mL of deionized water, shaking, uniformly mixing, standing for 5 minutes, putting 0.5mL of supernatant into a 2mL centrifuge tube, and adding 1 drop of 1.0mol/L hydrochloric acid and 2 drops of 2.3mol/L potassium iodide solution. After shaking and mixing, the mixture is placed for 1 minute at room temperature and then is compared with a standard color card I for detecting potassium bromate to roughly quantify.
Example 5
Peeling off the skin of the bread, putting 1g of white part into a 15mL centrifuge tube, adding 7mL of deionized water, shaking, uniformly mixing, standing for 5 minutes, putting 0.5mL of soak solution into a 2mL centrifuge tube, and adding 1 drop of 0.5mol/L hydrochloric acid and 1 drop of 1.8mol/L potassium iodide solution. Shaking and mixing evenly and comparing with the standard color card II for potassium bromate detection to roughly quantify.
Example 6
Peeling off the skin of the bread, putting 1g of white part into a 15mL centrifuge tube, adding 7mL of deionized water, shaking, uniformly mixing, standing for 5 minutes, putting 0.5mL of soak solution into a 2mL centrifuge tube, and adding 1 drop of 0.5mol/L hydrochloric acid and 1 drop of 2.3mol/L potassium iodide solution. After shaking and mixing evenly, the mixture is compared with a standard color card II for potassium bromate detection to be roughly quantified.
Example 7
Peeling off the skin of the bread, putting 1g of white part into a 15mL centrifuge tube, adding 7mL of deionized water, shaking, uniformly mixing, standing for 5 minutes, putting 0.5mL of soak solution into a 5mL centrifuge tube, and adding 1 drop of 1.0mol/L hydrochloric acid and 1 drop of 1.8mol/L potassium iodide solution. After shaking and mixing, the mixture is compared with a standard color card II for potassium bromate detection at room temperature to be roughly quantified.
Example 8
Peeling off the skin of the bread, putting 1g of white part into a 15mL centrifuge tube, adding 7mL of deionized water, shaking, uniformly mixing, standing for 5 minutes, putting 0.5mL of soak solution into a 5mL centrifuge tube, and adding 1 drop of 1.0mol/L hydrochloric acid and 1 drop of 2.3mol/L potassium iodide solution. After shaking and mixing uniformly, the potassium bromate detection standard color card II is relatively roughly quantified.
In summary, the invention provides a detection reagent and a kit for rapidly detecting potassium bromate, wherein the detection reagent is used for rapidly detecting potassium bromate in food, and comprises the following components: the detection reagent A is 0.5-1.0mol/L inorganic acid, and the detection reagent B is 1.8-2.3mol/L potassium iodide solution. When the pH value of the reagent is 1-3, the reagent has the advantages of short color development time, stable color, high detectable rate and no need of avoiding light in the color development process. The rapid detection requirement can be met.
Further, the invention also provides a kit for rapidly detecting potassium bromate, which comprises: detection reagent A, detection reagent B, centrifuging tube, color comparison cardboard and pipette etc.. When the detection is needed, the sample to be detected is firstly processed, the detection reagent A and the detection reagent B are added into the processed sample, the processed sample is mixed and then stands still, then the color is compared with the color level on the color comparison card, and whether the sample to be detected contains the potassium bromate or not is obtained according to the comparison result. Through comparison tests, the detection limit of the detection kit provided by the invention is 5 mg/kg.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. A detection reagent for rapidly detecting potassium bromate is used for rapidly detecting the potassium bromate in food, and is characterized by comprising the following components: the detection reagent A is 0.5-1.0mol/L inorganic acid, and the detection reagent B is 1.8-2.3mol/L potassium iodide solution.
2. The detection reagent for rapidly detecting potassium bromate according to claim 1, wherein the inorganic acid comprises: hydrochloric acid, sulfuric acid and glacial acetic acid.
3. The detection reagent for rapidly detecting potassium bromate according to claim 1, wherein the detection limit of the detection reagent is 5 mg/kg.
4. A kit for rapidly detecting potassium bromate, comprising the detection reagent according to any one of claims 1 to 3.
5. The kit of claim 4, wherein the kit further comprises: box body, color comparison cardboard and centrifuging tube.
6. The kit of claim 5, wherein the colorimetric cardboard: the kit comprises a substrate and a standard colorimetric color scale fixed on the surface of the substrate and used for detecting potassium bromate.
7. A kit according to claim 6, wherein a recess is provided between two adjacent gradations for receiving a test tube.
8. The kit of claim 6, wherein the color gradation comprises: yellow, magenta, purple, and bluish-purple.
9. The kit of claim 5, wherein the colorimetric card comprises: the colorimetric card board I is used for detecting potassium bromate in the wheat flour and the colorimetric card board II is used for detecting potassium bromate in the bread.
10. The kit of claim 4, wherein the detection reagent B is contained in a black reagent bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011640239.7A CN112834492A (en) | 2020-12-31 | 2020-12-31 | Detection reagent and kit for rapidly detecting potassium bromate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011640239.7A CN112834492A (en) | 2020-12-31 | 2020-12-31 | Detection reagent and kit for rapidly detecting potassium bromate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112834492A true CN112834492A (en) | 2021-05-25 |
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| CN202011640239.7A Pending CN112834492A (en) | 2020-12-31 | 2020-12-31 | Detection reagent and kit for rapidly detecting potassium bromate |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101226139A (en) * | 2008-02-01 | 2008-07-23 | 河南兴泰科技实业有限公司 | Method for chemistry testing potassium bromate in flour, amendment or food |
| CN103245658A (en) * | 2013-04-23 | 2013-08-14 | 山西省食品质量安全监督检验研究院 | Fast detection method of bromate in bread |
| CN105510246A (en) * | 2015-11-26 | 2016-04-20 | 佛山市海天(高明)调味食品有限公司 | Method for judging soy sauce color grade through pigment standard colorimetric card |
| CN207423915U (en) * | 2017-11-08 | 2018-05-29 | 四川农业大学 | A kind of detection device of Bread Quality |
| CN110208260A (en) * | 2018-08-23 | 2019-09-06 | 智锐达仪器科技南通有限公司 | Potassium bromate quick detection reagent, kit and detection method in a kind of flour |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101226139A (en) * | 2008-02-01 | 2008-07-23 | 河南兴泰科技实业有限公司 | Method for chemistry testing potassium bromate in flour, amendment or food |
| CN103245658A (en) * | 2013-04-23 | 2013-08-14 | 山西省食品质量安全监督检验研究院 | Fast detection method of bromate in bread |
| CN105510246A (en) * | 2015-11-26 | 2016-04-20 | 佛山市海天(高明)调味食品有限公司 | Method for judging soy sauce color grade through pigment standard colorimetric card |
| CN207423915U (en) * | 2017-11-08 | 2018-05-29 | 四川农业大学 | A kind of detection device of Bread Quality |
| CN110208260A (en) * | 2018-08-23 | 2019-09-06 | 智锐达仪器科技南通有限公司 | Potassium bromate quick detection reagent, kit and detection method in a kind of flour |
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Application publication date: 20210525 |