CN112812887A - Peanut oil enzymatic degumming process - Google Patents
Peanut oil enzymatic degumming process Download PDFInfo
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- CN112812887A CN112812887A CN202011536345.0A CN202011536345A CN112812887A CN 112812887 A CN112812887 A CN 112812887A CN 202011536345 A CN202011536345 A CN 202011536345A CN 112812887 A CN112812887 A CN 112812887A
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- phospholipase
- peanut oil
- degumming process
- enzymatic degumming
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- 235000019483 Peanut oil Nutrition 0.000 title claims abstract description 40
- 239000000312 peanut oil Substances 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 18
- 230000002255 enzymatic effect Effects 0.000 title claims abstract description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 33
- 239000011259 mixed solution Substances 0.000 claims abstract description 20
- 238000003756 stirring Methods 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 235000011121 sodium hydroxide Nutrition 0.000 claims abstract description 11
- 230000001877 deodorizing effect Effects 0.000 claims abstract description 10
- 238000001035 drying Methods 0.000 claims abstract description 10
- 238000005406 washing Methods 0.000 claims abstract description 10
- 239000002253 acid Substances 0.000 claims abstract description 9
- 239000000654 additive Substances 0.000 claims abstract description 8
- 230000000996 additive effect Effects 0.000 claims abstract description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 24
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 claims description 14
- 235000013923 monosodium glutamate Nutrition 0.000 claims description 14
- 239000004223 monosodium glutamate Substances 0.000 claims description 14
- 102100031415 Hepatic triacylglycerol lipase Human genes 0.000 claims description 12
- 108010013563 Lipoprotein Lipase Proteins 0.000 claims description 12
- 102000011420 Phospholipase D Human genes 0.000 claims description 12
- 108090000553 Phospholipase D Proteins 0.000 claims description 12
- 102000015439 Phospholipases Human genes 0.000 claims description 12
- 108010064785 Phospholipases Proteins 0.000 claims description 12
- 102000014384 Type C Phospholipases Human genes 0.000 claims description 12
- 108010079194 Type C Phospholipases Proteins 0.000 claims description 12
- 102100037611 Lysophospholipase Human genes 0.000 claims description 11
- 108010058864 Phospholipases A2 Proteins 0.000 claims description 11
- 239000010460 hemp oil Substances 0.000 claims description 10
- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 claims description 9
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 6
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 3
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 2
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 2
- 239000011975 tartaric acid Substances 0.000 claims description 2
- 235000002906 tartaric acid Nutrition 0.000 claims description 2
- 239000003513 alkali Substances 0.000 claims 1
- 239000008157 edible vegetable oil Substances 0.000 abstract description 2
- 102000004882 Lipase Human genes 0.000 abstract 1
- 108090001060 Lipase Proteins 0.000 abstract 1
- 239000004367 Lipase Substances 0.000 abstract 1
- 238000009874 alkali refining Methods 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 abstract 1
- 235000019421 lipase Nutrition 0.000 abstract 1
- 238000006386 neutralization reaction Methods 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 11
- 150000003904 phospholipids Chemical class 0.000 description 11
- 235000017060 Arachis glabrata Nutrition 0.000 description 9
- 244000105624 Arachis hypogaea Species 0.000 description 9
- 235000010777 Arachis hypogaea Nutrition 0.000 description 9
- 235000018262 Arachis monticola Nutrition 0.000 description 9
- 240000006661 Serenoa repens Species 0.000 description 9
- 239000010779 crude oil Substances 0.000 description 9
- 235000020232 peanut Nutrition 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 5
- 239000011574 phosphorus Substances 0.000 description 5
- 229910052698 phosphorus Inorganic materials 0.000 description 5
- 235000019498 Walnut oil Nutrition 0.000 description 4
- 150000002978 peroxides Chemical class 0.000 description 4
- 239000008170 walnut oil Substances 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 3
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 229960004488 linolenic acid Drugs 0.000 description 3
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 235000019198 oils Nutrition 0.000 description 3
- 235000013305 food Nutrition 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 238000007670 refining Methods 0.000 description 2
- 239000008159 sesame oil Substances 0.000 description 2
- 235000011803 sesame oil Nutrition 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- PORPENFLTBBHSG-MGBGTMOVSA-N 1,2-dihexadecanoyl-sn-glycerol-3-phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(O)=O)OC(=O)CCCCCCCCCCCCCCC PORPENFLTBBHSG-MGBGTMOVSA-N 0.000 description 1
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- 244000187129 Bacopa monnieria Species 0.000 description 1
- 235000015418 Bacopa monnieria Nutrition 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 235000012939 Caryocar nuciferum Nutrition 0.000 description 1
- 240000004929 Juglans cinerea Species 0.000 description 1
- 235000014056 Juglans cinerea Nutrition 0.000 description 1
- 241000123241 Sparassis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229960005069 calcium Drugs 0.000 description 1
- 235000001465 calcium Nutrition 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 150000002433 hydrophilic molecules Chemical class 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229910001608 iron mineral Inorganic materials 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000000391 smoking effect Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/02—Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
- A23D9/04—Working-up
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/02—Refining fats or fatty oils by chemical reaction
- C11B3/04—Refining fats or fatty oils by chemical reaction with acids
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/02—Refining fats or fatty oils by chemical reaction
- C11B3/06—Refining fats or fatty oils by chemical reaction with bases
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Edible Oils And Fats (AREA)
- Fats And Perfumes (AREA)
Abstract
The invention relates to the technical field of edible oil processing, and provides a peanut oil enzymatic degumming process, which comprises the following steps: s1, adding acid into the crude peanut oil, and stirring and mixing uniformly; s2, adding caustic soda liquid into the mixed solution of S1, and stirring and mixing uniformly; s3, adding lipase, water and an additive into the mixed solution S2, uniformly mixing, and carrying out enzymolysis; s4, washing, centrifuging, drying and deodorizing to obtain the refined peanut oil. Through the technical scheme, the problem of high yield loss of the existing neutralization alkali-refining technology is solved.
Description
Technical Field
The invention relates to the technical field of edible oil processing, in particular to an enzymatic degumming process for peanut oil.
Background
The peanut has higher nutritive value and is a high-quality oil and protein resource. Phospholipids are an important component of biological membranes and are present in oil crops in large quantities, and the influence of phospholipids on the oxidative stability of lipids is greatly controversial. If the phospholipid content is high, foaming, smoking and odor are likely to occur during heating, and the phospholipid is oxidized at high temperature to turn brown, which affects the flavor of the fried food. Therefore, in the refining of peanut oil, phospholipid needs to be removed, and the technological process of removing peptized impurities in crude oil by using a physical, chemical or physical-chemical method is degumming.
Enzymatic degumming is another new degumming method, and the principle is that part of groups in phospholipid are hydrolyzed by phospholipase, so that phospholipid is changed into more hydrophilic molecules or polar groups of phospholipid are hydrolyzed, so that non-hydrated phospholipid is removed. At present, the degumming time of the enzymatic degumming process is long, the degumming effect is poor, and the phosphorus content of the obtained peanut oil is high.
Disclosure of Invention
The invention provides an enzymatic degumming process for peanut oil, which solves the problems of low degumming yield and long required time in the prior art.
The technical scheme of the invention is as follows:
an enzymatic degumming process for peanut oil, comprising the following steps:
s1, adding acid into the crude peanut oil, and stirring and mixing uniformly;
s2, adding caustic soda liquid into the mixed solution of S1, and stirring and mixing uniformly;
s3, adding phospholipase, water and an additive into the mixed solution S2, uniformly mixing, and carrying out enzymolysis;
s4, washing, centrifuging, drying and deodorizing to obtain the refined peanut oil.
As a further technical solution, in the step S1, the acid includes one or more of citric acid, phosphoric acid, and tartaric acid.
In a further technical scheme, in the step S1, the mass percentage concentration of the acid is 4-5%, and the mass ratio of the acid to the peanut crude oil is (0.2-0.4): 1.
As a further technical scheme, in the step S2, adding liquid caustic soda to adjust the pH value to 7-7.3.
As a further technical scheme, in the step S3, the mass ratio of the phospholipase, the additive, the water and the peanut crude oil is 1 (0.06-0.1): (4000-6000): (800-1000).
According to a further technical scheme, in the step S3, the additives comprise palmetto hemp oil and monosodium glutamate, and the mass ratio of the palmetto hemp oil to the monosodium glutamate is 1 (0.08-0.1).
In a further technical scheme, in the step S3, the phospholipase comprises phospholipase A1, phospholipase A2, phospholipase C and phospholipase D, and the mass ratio of the phospholipase A1, the phospholipase A2, the phospholipase C and the phospholipase D is 1 (0.4-0.6) to 1 (0.1-0.3).
As a further technical scheme, the temperature of the step S3 is controlled to be 45-50 ℃ and the time is 1-2 hours.
The principle and the beneficial effects of the invention are as follows:
1. the action sites of different phospholipases are different, and in the present invention, the phospholipase A1 acts on the unsaturated fatty acid ester bond S of phospholipidn-1Phospholipase A2 specific for Sn-2A bit. Phospholipase C, in turn, catalyzes primarily a reaction at the insoluble substrate at the lipid-water interface. Phospholipase D catalyzes the hydrolysis of phosphatidylcholine to choline and phosphatidic acid. According to the invention, the four phospholipases are added, and the proportion of the four phospholipases is determined through numerous tests, so that the obtained peanut oil has low phosphorus content and good degumming effect.
2. In the invention, the palmetto hemp oil is added to reduce the viscosity of the reaction system, ensure the full contact of phospholipase and improve the catalytic hydrolysis efficiency of enzyme. Meanwhile, the palmetto hemp oil contains lecithin, linolenic acid, vitamins, calcium, iron minerals and other trace elements necessary for human bodies, and the linolenic acid and the vitamins can play a role in catalyzing and strengthening the activity of enzyme, so that the enzymolysis efficiency is improved, and the degumming time is shortened. The addition of monosodium glutamate and metal elements in the palmetto hemp oil and walnut oil can promote enzymolysis reaction, enhance the activity of enzyme, improve the stability and oxidation resistance of the obtained peanut oil and ensure the food safety of the obtained peanut oil at the same time.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any inventive step, are intended to be within the scope of the present invention.
Example 1
S1, adding 160g of citric acid with the mass percentage concentration of 5% into 800g of peanut crude oil, and stirring and mixing uniformly;
s2, adding caustic soda liquid into the mixed solution of S1, stirring and mixing uniformly, and adjusting the pH value to 7;
s3, adding 0.4g of phospholipase A1, 0.16g of phospholipase A2, 0.4g of phospholipase C, 0.04g of phospholipase D, 4000g of water, 0.055g of palmetto hemp oil and 0.005g of monosodium glutamate into the mixed solution of S2, uniformly mixing, and carrying out enzymolysis, wherein the temperature is controlled to be 45-50 ℃ and the time is 1-2 hours;
s4, washing, centrifuging, drying and deodorizing to obtain the refined peanut oil.
Example 2
S1, adding 270g of phosphoric acid with the mass percentage concentration of 4% into 900g of crude peanut oil, and stirring and mixing uniformly;
s2, adding caustic soda liquid into the mixed solution of S1, stirring and mixing uniformly, and adjusting the pH value to 7.2;
s3, adding 0.37g of phospholipase A1, 0.18g of phospholipase A2, 0.4g of phospholipase C, 0.04g of phospholipase D, 5000g of water, 0.073g of palmetto sesame oil and 0.007g of monosodium glutamate into the mixed solution of S2, uniformly mixing, and carrying out enzymolysis, wherein the temperature is controlled to be 45-50 ℃ and the time is 1-2 hours;
s4, washing, centrifuging, drying and deodorizing to obtain the refined peanut oil.
Example 3
S1, adding 400g of citric acid with the mass percentage concentration of 5% into 1000g of peanut crude oil, and stirring and mixing uniformly;
s2, adding caustic soda liquid into the mixed solution of S1, stirring and mixing uniformly, and adjusting the pH value to 7.3;
s3, adding 0.35g of phospholipase A1, 0.21g of phospholipase A2, 0.35g of phospholipase C, 0.09g of phospholipase D, 6000g of water, 0.092g of Bama hemp oil and 0.008g of monosodium glutamate into the mixed solution of S2, uniformly mixing, and carrying out enzymolysis, wherein the temperature is controlled to be 45-50 ℃ and the time is 1-2 hours;
s4, washing, centrifuging, drying and deodorizing to obtain the refined peanut oil.
Example 4
S1, adding 400g of citric acid with the mass percentage concentration of 5% into 1000g of peanut crude oil, and stirring and mixing uniformly;
s2, adding caustic soda liquid into the mixed solution of S1, stirring and mixing uniformly, and adjusting the pH value to 7.3;
s3, adding 0.35g of phospholipase A1, 0.21g of phospholipase A2, 0.35g of phospholipase C, 0.09g of phospholipase D, 6000g of water, 0.067g of palmetto hemp oil, 0.026g of walnut oil and 0.006g of monosodium glutamate into the mixed solution of S2, uniformly mixing, and performing enzymolysis at the temperature of 45-50 ℃ for 1-2 hours;
s4, washing, centrifuging, drying and deodorizing to obtain the refined peanut oil.
Comparative example 1
S1, adding 400g of citric acid with the mass percentage concentration of 5% into 1000g of peanut crude oil, and stirring and mixing uniformly;
s2, adding caustic soda liquid into the mixed solution of S1, stirring and mixing uniformly, and adjusting the pH value to 7.3;
s3, adding 0.35g of phospholipase A1, 0.21g of phospholipase A2, 0.35g of phospholipase C, 0.09g of phospholipase D, 6000g of water and 0.01g of monosodium glutamate into the mixed solution of S2, uniformly mixing, and carrying out enzymolysis, wherein the temperature is controlled to be 45-50 ℃ and the time is 1-2 hours;
s4, washing, centrifuging, drying and deodorizing to obtain the refined peanut oil.
Comparative example 2
S1, adding 400g of citric acid with the mass percentage concentration of 5% into 1000g of peanut crude oil, and stirring and mixing uniformly;
s2, adding caustic soda liquid into the mixed solution of S1, stirring and mixing uniformly, and adjusting the pH value to 7.3;
s3, adding 0.35g of phospholipase A1, 0.21g of phospholipase A2, 0.35g of phospholipase C, 0.09g of phospholipase D, 6000g of water and 0.01g of monosodium glutamate into the mixed solution of S2, uniformly mixing, and carrying out enzymolysis, wherein the temperature is controlled to be 45-50 ℃ and the time is 1-2 hours;
s4, washing, centrifuging, drying and deodorizing to obtain the refined peanut oil.
Comparative example 3
S1, adding 400g of citric acid with the mass percentage concentration of 5% into 1000g of peanut crude oil, and stirring and mixing uniformly;
s2, adding caustic soda liquid into the mixed solution of S1, stirring and mixing uniformly, and adjusting the pH value to 7.3;
s3, adding 0.35g of phospholipase A1, 0.21g of phospholipase A2, 0.35g of phospholipase C, 0.09g of phospholipase D and 6000g of water into the mixed solution of S2, uniformly mixing, and carrying out enzymolysis, wherein the temperature is controlled to be 45-50 ℃ and the time is 1-2 hours;
s4, washing, centrifuging, drying and deodorizing to obtain the refined peanut oil.
The peanut oil obtained by refining is measured according to the peroxide value of GB/T5538-: detecting the phosphorus content and obtaining the peanut oil.
TABLE 1 test data for examples 1-4 and comparative examples 1-3
| Detecting items | Peanut oil phosphorus content/ppm | Peroxide number mmol/kg | Yield of peanut oil/%) |
| Example 1 | 10.2 | 1.3 | 98.6 |
| Example 2 | 11.6 | 1.5 | 98.1 |
| Example 3 | 9.5 | 1 | 98.9 |
| Example 4 | 8.0 | 0.7 | 99.1 |
| Comparative example 1 | 23.6 | 1.9 | 97.2 |
| Comparative example 2 | 26.4 | 2.2 | 96.9 |
| Comparative example 3 | 36.7 | 2.7 | 96.6 |
The applicant has found that when a small amount of walnut oil is added to the additive comprising the combination of butternut oil and monosodium glutamate, as in example 5, the effect of the present invention is superior to that of the other examples of the present invention, and that linolenic acid in the walnut oil can promote more and more sufficient contact between phospholipids and phospholipase, thereby enhancing the enzymatic hydrolysis.
Comparing comparative example 1 with example 3, it was found that when no Bacopa hemp oil was added, the refined peanut oil was obtained in a lower yield, with a higher phosphorus content and a higher peroxide value. The yield, degumming effect and peroxide number of the obtained peanut oil were worse than in comparative example 1 when no monosodium glutamate was added in comparative example 2, and the effect was the worst when neither of the sparassis sesame oil and monosodium glutamate was added in comparative example 3. Therefore, the invention creatively combines the palmetto and the monosodium glutamate together to catalyze the phospholipid enzymolysis, and achieves unexpected effects.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions, improvements, etc. within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. The peanut oil enzymatic degumming process is characterized by comprising the following steps of:
s1, adding acid into the crude peanut oil, and stirring and mixing uniformly;
s2, adding caustic soda liquid into the mixed solution of S1, and stirring and mixing uniformly;
s3, adding phospholipase, water and an additive into the mixed solution S2, uniformly mixing, and carrying out enzymolysis;
s4, washing, centrifuging, drying and deodorizing to obtain the refined peanut oil.
2. The enzymatic degumming process according to claim 1, wherein in step S1, the acid comprises one or more of citric acid, phosphoric acid, tartaric acid.
3. The peanut oil enzymatic degumming process according to claim 1, wherein in step S1, the mass percentage concentration of the acid is 4-5%, and the mass ratio of the acid to the crude peanut oil is (0.2-0.4): 1.
4. The enzymatic degumming process for peanut oil according to claim 1, wherein in step S2, liquid alkali is added to adjust the pH to 7-7.3.
5. The enzymatic degumming process for peanut oil according to claim 1, wherein in the step S3, the mass ratio of the phospholipase, the additive, the water and the crude peanut oil is 1 (0.06-0.1): 4000-6000): 800-1000.
6. The peanut oil enzymatic degumming process according to claim 1, wherein in step S3, the additive comprises Bama hemp oil and monosodium glutamate in a mass ratio of 1 (0.08-0.1).
7. The peanut oil enzymatic degumming process as claimed in claim 1, wherein in step S3, the phospholipase comprises phospholipase A1, phospholipase A2, phospholipase C, and phospholipase D, and the mass ratio of the phospholipase A1, the phospholipase A2, the phospholipase C, and the phospholipase D is 1 (0.4-0.6) to 1 (0.1-0.3).
8. The peanut oil enzymatic degumming process according to claim 1, wherein the temperature of step S3 is controlled at 45-50 ℃ for 1-2 hours.
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| CN107674750A (en) * | 2017-09-08 | 2018-02-09 | 安徽嘉旗粮油工程技术有限公司 | A kind of heavy fragrant peanut oil degumming and refining method |
| US20180305635A1 (en) * | 2017-04-25 | 2018-10-25 | Bunge Oils, Inc. | Process for enzymatic degumming |
| CN109370775A (en) * | 2018-12-07 | 2019-02-22 | 山东鲁花集团有限公司 | A kind of dephosphorization method using high speed shear vortex mixing auxiliary biological enzyme |
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| US20180305635A1 (en) * | 2017-04-25 | 2018-10-25 | Bunge Oils, Inc. | Process for enzymatic degumming |
| CN107674750A (en) * | 2017-09-08 | 2018-02-09 | 安徽嘉旗粮油工程技术有限公司 | A kind of heavy fragrant peanut oil degumming and refining method |
| CN109370775A (en) * | 2018-12-07 | 2019-02-22 | 山东鲁花集团有限公司 | A kind of dephosphorization method using high speed shear vortex mixing auxiliary biological enzyme |
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