CN112805371A - 基于CRISPR/Cas系统对细胞进行基因编辑的方法 - Google Patents

基于CRISPR/Cas系统对细胞进行基因编辑的方法 Download PDF

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CN112805371A
CN112805371A CN201980058264.3A CN201980058264A CN112805371A CN 112805371 A CN112805371 A CN 112805371A CN 201980058264 A CN201980058264 A CN 201980058264A CN 112805371 A CN112805371 A CN 112805371A
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cell
gene
antigen
grna
receptor
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李宗海
廖朝晖
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Clegg Medical Co ltd
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Cafa Therapeutics Ltd
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Abstract

提供了一种基于CRISPRlCas系统对细胞进行基因编辑的方法，其中Cas酶为酶活力为0‑1nmoI的Cas9酶。还提供了一种构建通用型T细胞的方法，制备得到的T细胞及其用途，其中通过基因编辑技术对T细胞的TCR基因和MHC基因进行基因编辑。还提供了一种gRNA构建体。

Description

PCT国内申请，说明书已公开。

Claims (30)

  1. PCT国内申请，权利要求书已公开。
CN201980058264.3A 2018-09-21 2019-09-23 基于CRISPR/Cas系统对细胞进行基因编辑的方法 Pending CN112805371A (zh)

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Application Number Priority Date Filing Date Title
CN201811117875 2018-09-21
CN2018111178754 2018-09-21
CN201811140870 2018-09-28
CN2018111408703 2018-09-28
CN201910809598 2019-08-29
CN2019108094758 2019-08-29
CN201910809475 2019-08-29
CN2019108095981 2019-08-29
PCT/CN2019/107374 WO2020057668A1 (zh) 2018-09-21 2019-09-23 基于CRISPR/Cas系统对细胞进行基因编辑的方法

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CN201980058264.3A Pending CN112805371A (zh) 2018-09-21 2019-09-23 基于CRISPR/Cas系统对细胞进行基因编辑的方法

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EP (1) EP3854871A4 (zh)
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KR (1) KR20210065141A (zh)
CN (2) CN112739817A (zh)
AU (1) AU2019341365A1 (zh)
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Cited By (4)

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Publication number Priority date Publication date Assignee Title
CN113827731A (zh) * 2021-09-22 2021-12-24 北京远胜达生物科技发展有限公司 Crispr基因编辑联合pd-1抗体在治疗癌症中的应用
WO2023066243A1 (zh) * 2021-10-18 2023-04-27 西安宇繁生物科技有限责任公司 一种将目的基因定点整合至免疫细胞特定位点的方法及其应用
WO2023193800A1 (zh) * 2022-04-07 2023-10-12 恺兴生命科技(上海)有限公司 嵌合多肽及其应用
WO2023202116A1 (zh) * 2022-04-20 2023-10-26 山东舜丰生物科技有限公司 Cas酶和系统以及应用

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AU2018209012A1 (en) * 2017-01-23 2019-09-12 Crage Medical Co., Limited BCMA-targeting antibody and use thereof
US20230054266A1 (en) * 2019-12-30 2023-02-23 Edigene Biotechnology Inc. Method for purifying ucart cell and use thereof
CN112522206A (zh) * 2020-12-15 2021-03-19 苏州恒康生命科学有限公司 一种ror1基因敲除肿瘤细胞株的构建方法及其应用
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