CN112798568A - Portable fluorescence detection device for leaf surface fog drops - Google Patents
Portable fluorescence detection device for leaf surface fog drops Download PDFInfo
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- CN112798568A CN112798568A CN202110142661.8A CN202110142661A CN112798568A CN 112798568 A CN112798568 A CN 112798568A CN 202110142661 A CN202110142661 A CN 202110142661A CN 112798568 A CN112798568 A CN 112798568A
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- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 31
- 230000007246 mechanism Effects 0.000 claims abstract description 12
- 238000012545 processing Methods 0.000 claims abstract description 12
- 230000002087 whitening effect Effects 0.000 claims abstract description 10
- 238000003825 pressing Methods 0.000 claims abstract description 8
- 230000003287 optical effect Effects 0.000 claims abstract description 4
- 238000001514 detection method Methods 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 8
- 230000005284 excitation Effects 0.000 claims description 4
- 238000003860 storage Methods 0.000 claims description 3
- 239000000700 radioactive tracer Substances 0.000 claims description 2
- 230000011218 segmentation Effects 0.000 claims description 2
- 229910001220 stainless steel Inorganic materials 0.000 claims description 2
- 239000010935 stainless steel Substances 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims 7
- 238000011895 specific detection Methods 0.000 claims 1
- 230000008021 deposition Effects 0.000 abstract description 5
- 238000005259 measurement Methods 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 7
- 230000031700 light absorption Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 3
- 229940043267 rhodamine b Drugs 0.000 description 3
- 229910052782 aluminium Inorganic materials 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000017525 heat dissipation Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000009718 spray deposition Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 1
- 244000203593 Piper nigrum Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002073 fluorescence micrograph Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a portable fluorescence detection device for leaf surface fog drops, which mainly comprises: the device comprises a light path, a blade spreading mechanism, a fixed platform, a shell and a fluorescence detection system. The light path part comprises an LED light source, a convex lens, a concave lens, an optical filter and a spectroscope; the blade spreading mechanism mainly comprises a whitening lens, a telescopic rod and a cam rapid pressing mechanism; the shell mainly comprises a spring hinge, a push switch and a flip cover; the fluorescence detection system is built based on a smart phone, and functions of photographing, image processing, data calculation and the like are realized in developed mobile phone APP software. The invention adopts the design of a reflection type confocal light path, focuses a light source and reduces the volume of the device; a blade spreading mechanism is adopted, so that the problem of uneven reflection caused by blade convolution is solved, and nondestructive blade droplet deposition measurement is realized; and a fluorescence detection system is adopted to realize real-time online measurement.
Description
Technical Field
The invention discloses a portable fluorescence detection device for leaf surface fog drops, which does not need to pick leaves and can detect in real time, is used for researching the deposition distribution of the fog drops sprayed on leaf surfaces, relates to the technical field of fluorescence detection, particularly relates to a portable fluorescence detection device for the leaf surface fog drops, and belongs to the technical field of plant protection.
Background
Plant protection is a very important link in agricultural production, and methods for plant protection include chemical control, physical control, biological control and the like. The chemical prevention and control has the characteristics of high efficiency, timely prevention and control and the like, and can control sudden and large-area outbreak of plant diseases and insect pests in time, so the chemical prevention and control method is the most widely applied plant protection method at present. Chemical control is achieved by spraying the plant, and the spray deposition distribution is one of the criteria for evaluating the effect of the application.
Most of the existing fluorescence detection devices are devices for detecting leaves by aiming at water-sensitive test paper, solution and solid, and the fluorescence detection devices for processing leaf images by aiming at leaf areas are not researched by people. The existing device for detecting the fogdrop deposition is mainly used for sampling devices such as water-sensitive test paper, polyester cards and the like, the sampling devices need to be distributed and collected, and then a series of processing is carried out to obtain data, the process is complex, compared with actual blade measurement, the device has errors, and real-time detection cannot be carried out. Due to the characteristics of different sizes, different shapes, different colors and the like of the leaves, the image processing of the fog drops sprayed on the leaves is difficult and the effect is not ideal.
At present, some fluorescence detection devices are available, and chinese patent CN201520319714.9 discloses a fluorescence detection device, which utilizes the confocal light path principle to realize portability of the fluorescence detection device, but the device cannot detect fog drops on leaves for trace amount of solution. Chinese patent cn201510448172.x discloses a portable fluorescence detection device, which realizes fluorescence detection of a test paper card, but the test paper card has certain limitations and cannot really replace leaves to perform fluorescence detection.
Disclosure of Invention
The invention provides a fluorescence detection device which can directly carry out nondestructive detection on plant leaves and can solve the problems of leaf curling and online image processing aiming at the defect that spray deposition direct detection cannot be carried out on the leaves.
In order to achieve the purpose, the technical scheme of the invention is as follows:
firstly, the leaves are put into the machine for fixation. An excitation light source is emitted by an LED lamp (5), and is condensed by a convex lens (2) and filtered by a filter (4), the excitation light is reflected to a blade to be detected by a spectroscope (1), fluorescent fog drops on the blade to be detected emit fluorescent light, and the fluorescent fog drops are imaged and processed in a fluorescent detection system (11) through the spectroscope (1), the filter (4) and a concave lens (3). The detected blade is flattened by a whitening lens (7), the whitening lens is fixed on a fixed platform (10) by a cam pressing mechanism (9), and the whitening lens (7) is supported by a telescopic rod (8) to move up and down. Through a fluorescence detection system (11) on developed smart phone APP software, functions such as photographing, image processing and data calculation are achieved.
Preferably, the telescopic rod (8) is a stainless steel telescopic rod with an eccentric device, the eccentric device is fixed at one end of the inner pipe inserted into the outer pipe, a movable eccentric sleeve is sleeved on the eccentric column, the inner pipe and the outer pipe rotate relatively, and the movable eccentric sleeve extrudes the inner wall of the outer pipe, so that locking is realized.
Preferably, the device shell (12) is made of a black PVC plastic plate which has good light absorption and no light transmission.
Preferably, the convex lens (2) is made movable. In the light path, a slide rail is added at the position where the convex lens (2) is placed, and the position of the convex lens (2) is slid according to the size of the blade, so that the size of a focused aperture is adjusted.
Preferably, the flip cover (121) is connected to the top with a spring hinge (122) and the bottom with a one-touch push button switch (123). When in use, the bottom switch is pressed, the flip cover (121) is bounced open, and then the blade is put in.
Preferably, the LED light source (5) adopts an independent power supply, and is really portable.
Preferably, the LED light source (5) is placed in a concave aluminum heat dissipation plate (6) to play a role in dissipating heat and gathering the light source.
Preferably, the image processing software is developed by the aid of a smart phone, the software has the functions of Hough circle detection, fogdrop adhesion segmentation and storage, and the problem that the smart phone does not have corresponding image processing software is solved.
Preferably, the developed software operation flow functions: and opening software, clicking to photograph to obtain a fluorescence image, clicking to process the image to obtain droplet deposition data, and clicking to store by using a storage key.
Preferably, an Acktar light absorption plate is used as the blade fixing platform (10), the Acktar light absorption plate has excellent light absorption performance, blades and a background can be distinguished obviously when image processing is carried out, and the blade area calculation accuracy rate is improved.
Drawings
Fig. 1 is a schematic diagram of a portable fluorescent detection device for leaf surface fog drops.
Fig. 2 is a view showing the structure of the housing.
Fig. 3 is a schematic view of a one-touch push button switch.
Fig. 4 is a schematic view of the housing being flipped open.
Fig. 5 is a schematic view of a spring hinge connecting a flip cover to a top plate.
Fig. 6 is a schematic view of the telescoping rod.
In the figure: 1-a spectroscope; 2-a convex lens; 3-a concave lens; 4-an optical filter; 5-LED light source; 6-aluminum heat dissipation plate; 7-a whitening lens; 8, a telescopic rod; 9-cam type rapid pressing mechanism; 10-a blade fixed platform; 11-a fluorescence detection system; 12-a housing; 13-darkroom; 121-a flip cover; 122-spring hinge; 123-push button switch.
Detailed Description
In order that the invention may be more fully understood, reference will now be made to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It is to be noted that all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
In the first figure, rhodamine B as a fluorescent tracer is sprayed on pepper plants to detect droplet deposition on the leaves, which is described as an embodiment.
In the embodiment, the LED lamp (5) with the wavelength of 465nm is selected as an excitation light source of rhodamine B, and the fluorescence range emitted by the rhodamine B is 578-610 nm.
In the present embodiment, the transmission band is selected: 580nm-1200nm cutoff band: a long wave pass filter (4) of 200nm-575nm is used as a fluorescence filter, and the transmission wave band is selected as follows: cut-off band of 350nm-525 nm: a short-wave pass filter (4) of 540nm-900nm is used as an exciting light filter.
In the embodiment, the convex lens (2) is fixed in a movable mode, and the distance is adjusted according to the size of the blade so as to adjust the size of the aperture reflected to the blade to be measured. The aperture radius size adjusting range is 1.5cm-3 cm.
In the present embodiment, the 45-degree beam splitter (1) is highly reflective in the wavelength range of 450nm to 540nm and highly transmissive in the wavelength range of 560nm to 600 nm.
In the embodiment, the LED light source (5) is an annular array light source, and the power of the LED lamp bead is 3W.
In this embodiment, the specific operation flow of the whole apparatus is as follows:
a) the switch button (123) is pressed to flick the flip cover (121), the detected blade is placed on the blade fixing platform (10), the whitening lens (7) is put down to press the blade, the cam (9) quickly compresses the lens (7), and the blade is flattened as far as possible under the force without damaging the blade.
b) And opening the LED light source (5) and adjusting the size of the aperture.
c) And processing the image of the smart phone to obtain data.
d) And loosening the cam pressing mechanism (9) after image processing, and taking out the blade to finish detection.
e) And replacing the blade and repeating the process.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. The utility model provides a fluorescence detection device of portable leaf surface droplet which characterized in that includes: the device comprises light paths (1, 2, 3, 4, 5), blade spreading mechanisms (7, 8, 9), a fixed platform (10), a shell (12) and a fluorescence detection system (11); the light path part comprises an LED lamp (5), a convex lens (3), a concave lens (2), a light filter (4) and a spectroscope (1), a light source is filtered by an exciting light filter (4), the convex lens (3) is focused, the spectroscope (1) is reflected to a blade with fluorescent fog drops, and reflected fluorescent light passes through the spectroscope (1), an emitting light filter (4) and the concave lens (2) and then is imaged in a fluorescent detection system (11); the blade spreading mechanisms (7, 8 and 9) mainly comprise whitening lenses (7), telescopic rods (8) and cam rapid pressing mechanisms (9), the whitening lenses (7) are connected with the fixed platform (10) through the telescopic rods (8), and the cam rapid pressing mechanisms (9) are arranged on two sides of the fixed platform and used for pressing the lenses (7); the shell (12) mainly comprises a spring hinge (122), a push switch (123) and a flip cover (121), wherein the flip cover (121) is connected with the top of the shell (12) through the spring hinge (122), and the bottom of the flip cover is connected through a one-key button switch (122); the fluorescence detection system (11) is built based on a smart phone, and functions of photographing, image processing and data calculation are achieved by using software developed in the smart phone.
2. The portable fluorescent detection device for foliar spray droplets of claim 1, wherein the LED light source (5) is an annular array light source, and the LED light source with different wavelengths can be replaced according to the detected fluorescent tracer.
3. The portable fluorescence detection device for the fog drops on the leaf surface as claimed in claim 1, wherein the convex lens (3) and the concave lens (2) are fixed in a movable manner, the convex lens and the concave lens are coaxial, the positions of the convex lens and the concave lens on the optical axis can be adjusted, and the positions can be adjusted according to the size of the detected blade, so that the adjustment of the aperture size is realized.
4. The portable fluorescence detection device for foliar spray of claim 1 wherein the device housing (12) is made of light absorbing, opaque black PVC plastic plate.
5. Device housing (12) according to claim 4, characterized in that it is designed with a flip lid, the flip lid (121) being connected with the top with a spring hinge (122) and the bottom with a one-click button switch (123). When in use, the bottom switch is pressed, the flip cover (121) is bounced open, and then the blade is put in.
6. The portable fluorescent detection device of foliar spray of claim 1, characterized in that the whitening lens (7) is raised and lowered with a stainless steel telescopic rod (8) with eccentric device.
7. The fluorescence detection device of portable foliar spray of claim 1 wherein the spectroscope (1) and the optical filter (4) are replaced according to excitation wavelength and fluorescence wavelength.
8. The fluorescence detection device of portable foliar spray of claim 1 wherein the leaf fixation platform (10) is an Acktar light absorbing plate.
9. The portable fluorescence detection device for the fogdrops on the leaf surfaces as claimed in claim 1, wherein the fluorescence detection system uses a smart phone to develop image processing software, and the software has the functions of Hough circle detection, fogdrops adhesion segmentation and storage.
10. A portable fluorescence detection device for foliar spray droplets, which is characterized in that a specific detection method using the portable fluorescence detection device for foliar spray droplets according to any one of claims 1 to 8 comprises the following steps:
a) the switch button (123) is pressed to flick the flip cover (121), the detected blade is placed on the blade fixing platform (10), the whitening lens (7) is placed down to press the blade, the cam (9) quickly compresses the lens (7), and the blade is leveled as far as possible under the force without damaging the blade;
b) the LED light source (5) is turned on, and the size of the aperture is adjusted;
c) processing the images of the smart phone to obtain data;
d) after image processing, loosening the cam pressing mechanism (9), and taking out the blade to finish detection;
e) and replacing the blade and repeating the process.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110142661.8A CN112798568A (en) | 2021-02-02 | 2021-02-02 | Portable fluorescence detection device for leaf surface fog drops |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110142661.8A CN112798568A (en) | 2021-02-02 | 2021-02-02 | Portable fluorescence detection device for leaf surface fog drops |
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| CN202110142661.8A Pending CN112798568A (en) | 2021-02-02 | 2021-02-02 | Portable fluorescence detection device for leaf surface fog drops |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114047167A (en) * | 2021-10-27 | 2022-02-15 | 江苏大学 | Portable device and method for measuring coverage rate of leaf surface fog drops |
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2021
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