CN112790250A - Infant formula milk powder for promoting intestinal health and preparation method thereof - Google Patents
Infant formula milk powder for promoting intestinal health and preparation method thereof Download PDFInfo
- Publication number
- CN112790250A CN112790250A CN202110180540.2A CN202110180540A CN112790250A CN 112790250 A CN112790250 A CN 112790250A CN 202110180540 A CN202110180540 A CN 202110180540A CN 112790250 A CN112790250 A CN 112790250A
- Authority
- CN
- China
- Prior art keywords
- infant formula
- intestinal health
- powder
- promoting
- milk
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 235000013350 formula milk Nutrition 0.000 title claims abstract description 61
- 239000000843 powder Substances 0.000 title claims abstract description 61
- 230000001737 promoting effect Effects 0.000 title claims abstract description 45
- 230000007413 intestinal health Effects 0.000 title claims abstract description 43
- 235000013336 milk Nutrition 0.000 title claims abstract description 40
- 239000008267 milk Substances 0.000 title claims abstract description 40
- 210000004080 milk Anatomy 0.000 title claims abstract description 40
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- 241001134770 Bifidobacterium animalis Species 0.000 claims abstract description 25
- 229940118852 bifidobacterium animalis Drugs 0.000 claims abstract description 24
- 229940062827 2'-fucosyllactose Drugs 0.000 claims abstract description 14
- HWHQUWQCBPAQQH-UHFFFAOYSA-N 2-O-alpha-L-Fucosyl-lactose Natural products OC1C(O)C(O)C(C)OC1OC1C(O)C(O)C(CO)OC1OC(C(O)CO)C(O)C(O)C=O HWHQUWQCBPAQQH-UHFFFAOYSA-N 0.000 claims abstract description 14
- HWHQUWQCBPAQQH-BWRPKUOHSA-N 2-fucosyllactose Chemical compound O[C@H]1[C@H](O)[C@H](O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@H]([C@H](O)CO)[C@H](O)[C@@H](O)C=O HWHQUWQCBPAQQH-BWRPKUOHSA-N 0.000 claims abstract description 14
- SNFSYLYCDAVZGP-UHFFFAOYSA-N UNPD26986 Natural products OC1C(O)C(O)C(C)OC1OC1C(OC2C(OC(O)C(O)C2O)CO)OC(CO)C(O)C1O SNFSYLYCDAVZGP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims description 14
- 235000020247 cow milk Nutrition 0.000 claims description 13
- 235000020251 goat milk Nutrition 0.000 claims description 12
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 9
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 8
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 claims description 8
- 235000016709 nutrition Nutrition 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 8
- 235000015112 vegetable and seed oil Nutrition 0.000 claims description 7
- 239000008158 vegetable oil Substances 0.000 claims description 7
- 235000019484 Rapeseed oil Nutrition 0.000 claims description 6
- 108010046377 Whey Proteins Proteins 0.000 claims description 6
- 102000007544 Whey Proteins Human genes 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 235000012424 soybean oil Nutrition 0.000 claims description 6
- 239000003549 soybean oil Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 235000019498 Walnut oil Nutrition 0.000 claims description 5
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 5
- 239000011707 mineral Substances 0.000 claims description 5
- 235000010755 mineral Nutrition 0.000 claims description 5
- 235000015097 nutrients Nutrition 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 5
- 239000008170 walnut oil Substances 0.000 claims description 5
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 4
- 235000021342 arachidonic acid Nutrition 0.000 claims description 4
- 229940114079 arachidonic acid Drugs 0.000 claims description 4
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 4
- 239000008101 lactose Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 230000035764 nutrition Effects 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims description 3
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 claims description 3
- 235000019743 Choline chloride Nutrition 0.000 claims description 3
- 239000005862 Whey Substances 0.000 claims description 3
- 229960003178 choline chloride Drugs 0.000 claims description 3
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims description 3
- -1 compound vitamin Chemical class 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 235000021323 fish oil Nutrition 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 239000002773 nucleotide Substances 0.000 claims description 3
- 125000003729 nucleotide group Chemical group 0.000 claims description 3
- 150000003904 phospholipids Chemical class 0.000 claims description 3
- 238000001694 spray drying Methods 0.000 claims description 3
- 229930003231 vitamin Natural products 0.000 claims description 3
- 235000013343 vitamin Nutrition 0.000 claims description 3
- 239000011782 vitamin Substances 0.000 claims description 3
- 229940088594 vitamin Drugs 0.000 claims description 3
- 235000021119 whey protein Nutrition 0.000 claims description 3
- 235000019486 Sunflower oil Nutrition 0.000 claims description 2
- 239000003623 enhancer Substances 0.000 claims description 2
- 238000009928 pasteurization Methods 0.000 claims description 2
- 239000002600 sunflower oil Substances 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 claims 1
- 229940009289 bifidobacterium lactis Drugs 0.000 claims 1
- 238000000265 homogenisation Methods 0.000 claims 1
- 230000035755 proliferation Effects 0.000 abstract description 9
- 230000036039 immunity Effects 0.000 abstract description 8
- 230000000968 intestinal effect Effects 0.000 abstract description 7
- 241000894006 Bacteria Species 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 208000015181 infectious disease Diseases 0.000 abstract description 4
- 235000013305 food Nutrition 0.000 abstract description 3
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 16
- 238000002474 experimental method Methods 0.000 description 12
- 235000020256 human milk Nutrition 0.000 description 12
- 210000004251 human milk Anatomy 0.000 description 12
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 11
- 210000003608 fece Anatomy 0.000 description 11
- 238000011161 development Methods 0.000 description 10
- 230000018109 developmental process Effects 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 230000012010 growth Effects 0.000 description 10
- 241000699670 Mus sp. Species 0.000 description 9
- 229920001542 oligosaccharide Polymers 0.000 description 9
- 150000002482 oligosaccharides Chemical class 0.000 description 9
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 7
- 241000186000 Bifidobacterium Species 0.000 description 6
- 241001591005 Siga Species 0.000 description 6
- 235000014113 dietary fatty acids Nutrition 0.000 description 6
- 239000000194 fatty acid Substances 0.000 description 6
- 229930195729 fatty acid Natural products 0.000 description 6
- 150000004665 fatty acids Chemical class 0.000 description 6
- 235000020778 linoleic acid Nutrition 0.000 description 6
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 6
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 229960004488 linolenic acid Drugs 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 241000186660 Lactobacillus Species 0.000 description 4
- AXQLFFDZXPOFPO-UHFFFAOYSA-N UNPD216 Natural products O1C(CO)C(O)C(OC2C(C(O)C(O)C(CO)O2)O)C(NC(=O)C)C1OC(C1O)C(O)C(CO)OC1OC1C(O)C(O)C(O)OC1CO AXQLFFDZXPOFPO-UHFFFAOYSA-N 0.000 description 4
- AXQLFFDZXPOFPO-UNTPKZLMSA-N beta-D-Galp-(1->3)-beta-D-GlcpNAc-(1->3)-beta-D-Galp-(1->4)-beta-D-Glcp Chemical compound O([C@@H]1O[C@H](CO)[C@H](O)[C@@H]([C@H]1O)O[C@H]1[C@@H]([C@H]([C@H](O)[C@@H](CO)O1)O[C@H]1[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O1)O)NC(=O)C)[C@H]1[C@H](O)[C@@H](O)[C@H](O)O[C@@H]1CO AXQLFFDZXPOFPO-UNTPKZLMSA-N 0.000 description 4
- 239000011248 coating agent Substances 0.000 description 4
- 238000000576 coating method Methods 0.000 description 4
- 210000001072 colon Anatomy 0.000 description 4
- IEQCXFNWPAHHQR-UHFFFAOYSA-N lacto-N-neotetraose Natural products OCC1OC(OC2C(C(OC3C(OC(O)C(O)C3O)CO)OC(CO)C2O)O)C(NC(=O)C)C(O)C1OC1OC(CO)C(O)C(O)C1O IEQCXFNWPAHHQR-UHFFFAOYSA-N 0.000 description 4
- USIPEGYTBGEPJN-UHFFFAOYSA-N lacto-N-tetraose Natural products O1C(CO)C(O)C(OC2C(C(O)C(O)C(CO)O2)O)C(NC(=O)C)C1OC1C(O)C(CO)OC(OC(C(O)CO)C(O)C(O)C=O)C1O USIPEGYTBGEPJN-UHFFFAOYSA-N 0.000 description 4
- 229940062780 lacto-n-neotetraose Drugs 0.000 description 4
- RBMYDHMFFAVMMM-PLQWBNBWSA-N neolactotetraose Chemical compound O([C@H]1[C@H](O)[C@H]([C@@H](O[C@@H]1CO)O[C@@H]1[C@H]([C@H](O[C@H]([C@H](O)CO)[C@H](O)[C@@H](O)C=O)O[C@H](CO)[C@@H]1O)O)NC(=O)C)[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O RBMYDHMFFAVMMM-PLQWBNBWSA-N 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 235000019198 oils Nutrition 0.000 description 4
- 235000020238 sunflower seed Nutrition 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000606125 Bacteroides Species 0.000 description 3
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical class OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 3
- 210000004556 brain Anatomy 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 235000021255 galacto-oligosaccharides Nutrition 0.000 description 3
- 150000003271 galactooligosaccharides Chemical class 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 238000001802 infusion Methods 0.000 description 3
- 229940039696 lactobacillus Drugs 0.000 description 3
- 210000005036 nerve Anatomy 0.000 description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 2
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 206010012735 Diarrhoea Diseases 0.000 description 2
- 241000588914 Enterobacter Species 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 2
- DLRVVLDZNNYCBX-UHFFFAOYSA-N Polydextrose Polymers OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(O)O1 DLRVVLDZNNYCBX-UHFFFAOYSA-N 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000004641 brain development Effects 0.000 description 2
- 210000000481 breast Anatomy 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 230000002490 cerebral effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 230000004438 eyesight Effects 0.000 description 2
- 230000002550 fecal effect Effects 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000008944 intestinal immunity Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 235000020200 pasteurised milk Nutrition 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 210000000813 small intestine Anatomy 0.000 description 2
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 208000004232 Enteritis Diseases 0.000 description 1
- 241000305071 Enterobacterales Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- 235000021314 Palmitic acid Nutrition 0.000 description 1
- 229920001100 Polydextrose Polymers 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 229930003316 Vitamin D Natural products 0.000 description 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 210000003792 cranial nerve Anatomy 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 150000002066 eicosanoids Chemical class 0.000 description 1
- 239000006153 eosin methylene blue Substances 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000011552 falling film Substances 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 210000004211 gastric acid Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 208000028774 intestinal disease Diseases 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 235000020978 long-chain polyunsaturated fatty acids Nutrition 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007087 memory ability Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000006872 mrs medium Substances 0.000 description 1
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 1
- 230000007830 nerve conduction Effects 0.000 description 1
- 210000004498 neuroglial cell Anatomy 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229960003966 nicotinamide Drugs 0.000 description 1
- 235000005152 nicotinamide Nutrition 0.000 description 1
- 239000011570 nicotinamide Substances 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 229940055726 pantothenic acid Drugs 0.000 description 1
- 235000019161 pantothenic acid Nutrition 0.000 description 1
- 239000011713 pantothenic acid Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000011772 phylloquinone Substances 0.000 description 1
- 239000001259 polydextrose Substances 0.000 description 1
- 229940035035 polydextrose Drugs 0.000 description 1
- 235000013856 polydextrose Nutrition 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 230000001242 postsynaptic effect Effects 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000003518 presynaptic effect Effects 0.000 description 1
- 150000003180 prostaglandins Chemical class 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 235000020185 raw untreated milk Nutrition 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 210000001525 retina Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000011781 sodium selenite Substances 0.000 description 1
- 235000015921 sodium selenite Nutrition 0.000 description 1
- 229960001471 sodium selenite Drugs 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 210000000225 synapse Anatomy 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 239000011715 vitamin B12 Substances 0.000 description 1
- 239000011716 vitamin B2 Substances 0.000 description 1
- 239000011726 vitamin B6 Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C21/00—Whey; Whey preparations
- A23C21/06—Mixtures of whey with milk products or milk components
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C21/00—Whey; Whey preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C21/00—Whey; Whey preparations
- A23C21/02—Whey; Whey preparations containing, or treated with, microorganisms or enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C21/00—Whey; Whey preparations
- A23C21/08—Whey; Whey preparations containing other organic additives, e.g. vegetable or animal products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C21/00—Whey; Whey preparations
- A23C21/10—Whey; Whey preparations containing inorganic additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/515—Animalis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Inorganic Chemistry (AREA)
- Microbiology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention belongs to the technical field of food, and discloses infant formula milk powder for promoting intestinal health and a preparation method thereof. The 2' -fucosyllactose, the lactose-N-tetrasaccharide, the lactose-N-neotetraose and the bifidobacterium animalis subsp lactis i797 are added, so that the synergistic effect can be realized, the intestinal beneficial bacteria proliferation is promoted, the feed is suitable for infants, the intestinal flora of the infants can be adjusted, the immunity of the infants is enhanced, and the disease infection rate of the infants is reduced.
Description
Technical Field
The invention belongs to the technical field of food, and relates to infant formula milk powder, in particular to infant formula milk powder for promoting intestinal health and a preparation method thereof.
Background
Human Milk Oligosaccharides (HMOs) are an important component of Human Milk, and are the second largest carbohydrate ingredient and the third largest nutrient ingredient which are second only to lactose, and the content of the HMOs in Human Milk is between 5 and 15 g/L. HMOs can support colonization of infant intestinal flora, inhibit pathogen infection, promote immunity and brain development, and play an important role in infant growth and development. HMOs are of a wide variety, and over 200 have been found to be present, with oligosaccharides of a much lower variety and content in cow's milk than in breast milk. The oligosaccharides in breast milk can be classified into 3 major groups, fucosylated oligosaccharides, sialylated oligosaccharides and nonfucosylated neutral oligosaccharides.
As the breast-feeding rate of infants in China is low, investigation shows that the pure breast-feeding rate of infants within half a year of birth in China only reaches 20.8 percent and is lower than the average level of the world. The infant formula milk powder is particularly important as a breast milk substitute in the infant population lacking breast feeding. Existing infant formulas are often supplemented with indigestible carbohydrates (NDC) having similar effects as certain HMOs, such as galacto-oligosaccharides (GOS), fructo-oligosaccharides (FOS), GOS/FOS mixtures, polydextrose, etc. However, NDC cannot replace all the functions of HMOs. Because various systems of infants are not developed and mature, the infants are easy to be infected, and the phenomena of incomplete intestinal flora and low immunity are easy to occur when the infants eat the conventional infant formula milk powder.
Disclosure of Invention
The invention aims to provide infant formula milk powder for promoting intestinal health so as to realize the functions of regulating infant intestinal flora and enhancing infant immunity;
it is another object of the present invention to provide a method for preparing the above infant formula for promoting intestinal health.
In order to achieve the purpose, the invention adopts the technical scheme that:
an infant formula for promoting gut health comprising bifidobacterium animalis subsp lactis i 797;
the effective components of the infant formula milk powder for promoting intestinal health comprise the following raw materials in parts by weight:
60-70 parts of cow milk/goat milk;
② nutritional agent: 9-12 parts of lactose, 8-10 parts of desalted whey powder, 6-8 parts of vegetable oil, 4-5 parts of 1, 3-dioleate-2-palmitic acid triglyceride, 1-3 parts of concentrated whey protein powder, 1-1.5 parts of 2' -fucosyllactose, 0.5-0.8 part of lactose-N-tetrasaccharide, 0.2-0.5 part of phospholipid, 0.15-0.3 part of lactose-N-neotetrasaccharide, 0.1-0.3 part of compound mineral substance, 0.1-0.15 part of compound vitamin, 0.05-0.1 part of calcium carbonate, 0.05-0.10 part of potassium hydroxide and 0.05-0.10 part of choline chloride;
③ nutrient enriching agent: 0.2-0.4 part of arachidonic acid powder, 0.1-0.2 part of fish oil powder and 0.01-0.05 part of nucleotide.
As a limitation, the raw materials for preparing the effective components of the infant formula milk powder for promoting intestinal health also comprise 0.01-0.05 parts of bifidobacterium animalis subsp lactis i797 bacterial powder;
bifidobacterium animalis subsp.lactis i797(Bifidobacterium animalis subsp. lactis i797) has been deposited at 20.8.8.2019 at the China general microbiological culture Collection center (national institute of patent and microorganism Collection) of China Committee for culture Collection, national institute of culture Collection, No. 3, West Lu No.1, North Cheng, and south Kogyo, Beijing, with the deposit numbers: CGMCC No.18403, which was first disclosed in a prior patent application No. 202010259210.8.
As another limitation, the content of Bifidobacterium animalis subsp lactis i797 in the infant formula for promoting intestinal health is 1 × 109~1×1010CFU/g。
As a third limitation, the vegetable oil is prepared by mixing 3-5 parts by weight of soybean oil, 2-4 parts by weight of rapeseed oil, 1-2 parts by weight of sunflower seed oil and 1-2 parts by weight of walnut oil.
As a fourth limitation, the cow/goat milk is prepared by standardizing raw cow/goat milk and separating impurities.
The invention also provides a preparation method of the infant formula milk powder for promoting intestinal health, which comprises the steps of pasteurizing cow milk/goat milk, adding a nutrient, uniformly mixing, homogenizing, sterilizing, concentrating, spray drying, adding a nutrition enhancer and i797 strain powder of bifidobacterium animalis subsp.
As a limitation, the pasteurization temperature is 60-70 ℃ and the time is 15-18 s;
the sterilization temperature is 85-90 ℃ and the sterilization time is 22-30 s.
As a second limitation, adding a nutritional agent, uniformly mixing, and preheating to 60-65 ℃ before homogenizing; the homogenizing pressure is 16-18 MPa.
As a third limitation, after concentration, the density is 1090-1140 kg/m3The concentrated milk of (1).
And as a fourth limitation, the air inlet temperature of the spray drying is 165-180 ℃, and the air outlet temperature is 80-90 ℃.
Due to the adoption of the technical scheme, compared with the prior art, the invention has the technical progress that:
the infant formula milk powder for promoting intestinal health is added with three breast milk oligosaccharides (HMOs) of 2' -fucosyllactose, lactose-N-tetraose and lactose-N-neotetraose, the three oligosaccharides enter a baby body and cannot be damaged by gastric acid, and reach small intestine and colon in an integral form, so that the effect of prebiotics is exerted, the proliferation of bifidobacteria is promoted, lactic acid and acetic acid are generated to reduce the pH value of intestinal tracts, and pathogenic bacteria are inhibited; the three oligosaccharides can also prevent the combination of pathogenic bacteria and surface receptors of epithelial cells of organisms, enhance the immunity and reduce the infection rate of diseases of infants;
the soybean oil, the rapeseed oil, the sunflower seed oil and the walnut oil are added into the infant formula milk powder for promoting intestinal health, the linoleic acid content in cow milk/goat milk is far lower than that of the cow milk, the linoleic acid content of the soybean oil and the sunflower seed oil is higher, the linoleic acid content in cow milk/goat milk can be supplemented, the rapeseed oil contains rich unsaturated fatty acid oleic acid and linoleic acid, and a certain amount of alpha-linolenic acid, and the proportion of the necessary fatty acid linoleic acid and the alpha-linolenic acid is adjusted through the compounding of several kinds of vegetable oil; in addition, 1, 3-dioleic acid-2-palmitic acid triglyceride is added into the infant formula milk powder, so that the content of sn-2-position palmitic acid is increased, DHA and ARA are added, the proportion of long-chain polyunsaturated fatty acid is increased, the composition of the infant formula milk powder is closer to that of fatty acid in breast milk, nutrition required by growth and development of infants is ensured, and the vision and brain development of infants are promoted;
the bifidobacterium animalis subsp lactis i797 is added into the infant formula milk powder for promoting intestinal health, the bifidobacterium animalis subsp lactis i797 is screened and separated from feces of a breast-fed healthy infant, and has the effects of improving diarrhea and regulating dyspepsia, after three kinds of HMOs (2' -fucosyllactose, lactose-N-tetrasaccharide and lactose-N-neotetrasaccharide) enter small intestines, the proliferation of the bifidobacterium animalis subsp lactis i797 can be promoted, the proliferation of bifidobacterium can enhance the intestinal immunity, and the incidence rate of baby diarrhea is reduced;
in conclusion, the infant formula milk powder for promoting intestinal health disclosed by the invention is closer to breast milk, so that the nutrition required by growth and development of infants is ensured, the healthy development of intestinal tracts can be promoted, and the immunity of infants is enhanced.
The infant formula milk powder for promoting intestinal health is suitable for infants.
Drawings
FIG. 1 is a comparison graph of OD values of Bifidobacterium animalis lactococcus species i797 after culture in control group and experimental groups 11 to 16 in example 8 of the present invention;
FIG. 2 is a graph showing a comparison of the concentration of sIgA in feces of control group and experimental groups 21 to 26 in example 8 of the present invention;
FIG. 3 is a comparison graph of pH values of feces of control group and experimental groups 21 to 26 in example 8 of the present invention;
FIG. 4 is a graph showing the comparison of the content of the bacterial population in feces of the control group and the experimental groups 21 to 26 in example 8 of the present invention.
Detailed Description
The present invention is further illustrated by the following specific examples, which are to be construed as merely illustrative, and not limitative of the remainder of the disclosure.
Example 1A method of preparing an infant formula for promoting intestinal health
The process of this example for preparing an infant formula for promoting gut health, comprising the following steps performed in sequence
1) Checking and accepting, and cleaning milk
Standardizing raw milk, and then separating impurities to obtain milk;
2) pregabacide
Pasteurizing 60kg of cow milk at 65 ℃ for 18s, and cooling to room temperature to obtain pasteurized milk;
3) mixing material
3.55kg of soybean oil, 2.67kg of rapeseed oil, 0.89kg of sunflower oil and 0.89kg of walnut oil are taken as the total vegetable oil. Wherein, the proportion relation LB1 of soybean oil, rapeseed oil, sunflower seed oil and walnut oil is 4: 3: 1: 1;
taking 105g of calcium carbonate, 66g of calcium chloride, 51.3g of potassium chloride, 36g of copper sulfate, 15g of sodium chloride, 12g of potassium iodide, 3g of magnesium sulfate, 9g of manganese sulfate, 1.5g of sodium selenite, 0.3g of ferrous sulfate and 0.9g of zinc sulfate as composite minerals;
121.25g of vitamin C, 12.12g of vitamin E, 6.06g of pantothenic acid, 6.06g of nicotinamide and 2.27g of vitamin B20.64g vitamin A, 0.76g vitamin B10.61g of vitamin B60.08g folic acid, 0.045g vitamin K10.015g vitamin D, 0.045g vitamin B12And 0.045g biotin as a multivitamin;
12kg of lactose, 10kg of desalted whey powder, 8kg of vegetable oil, 5kg of 1, 3-dioleoyl-2-palmitic acid triglyceride powder, 3kg of concentrated whey protein powder, 1.2kg of 2' -fucosyllactose, 0.6kg of lactose-N-tetraose, 0.5kg of phospholipid, 0.2kg of lactose-N-neotetraose, 0.3kg of compound mineral, 0.15kg of compound vitamin, 0.1kg of calcium carbonate, 0.1kg of potassium hydroxide and 0.08kg of choline chloride are added into pasteurized milk, and the mixture is stirred, mixed uniformly and dissolved sufficiently to obtain feed liquid M1.
4) Preheating feed liquid M1 to 60 deg.C, homogenizing under 18MPa, heating to 85 deg.C for sterilizing for 30s, and concentrating by four-effect falling film evaporation to obtain solution with density of 1090kg/M3The concentrated milk of (1).
5) Pumping the concentrated milk into a dry state by a high-pressure pumpSpray drying in a drying tower at air inlet temperature of 180 deg.C and air exhaust temperature of 80 deg.C, adding 0.4kg arachidonic acid powder, 0.2kg fish oil powder, 0.05kg nucleotide and 0.05kg Bifidobacterium animalis subsp lactis i797 powder into the powder, stirring, and mixing completely to obtain infant formula milk powder (labeled as N1) for promoting intestinal health, wherein the content of Bifidobacterium animalis subsp lactis i797 is 1.0 × 1010CFU/g. The infant formula N1 for promoting intestinal health prepared by the embodiment is suitable for infants.
Example 2-6 preparation method of infant formula milk powder for promoting intestinal health
Examples 2 to 6 are methods of preparing infant formulas for promoting intestinal health, respectively, which have substantially the same procedure as in example 1, except for the differences in the amounts of raw materials and the control parameters associated with the operation process, as shown in tables 1 to 3:
table 1 raw material amount schedule of composite minerals
TABLE 2 raw material usage of multivitamins
Table 3 summary of raw material amounts and parameters in infant formula for promoting intestinal health
The contents of the other portions of examples 2 to 6 are the same as those of example 1.
Example 7 determination of fatty acid content of infant formula to promote gut health
The contents of linoleic acid (C18:2) and alpha-linolenic acid (C18:3) in the infant formula milk powder N1-N6 (12.9g of milk powder is added into 90mL of warm boiled water at about 50 ℃), breast milk (collected by volunteers), traditional formula milk powder 1 (prepared according to the specification), traditional formula goat milk powder 1 (prepared according to the specification) for promoting intestinal health prepared in the examples 1-6 are respectively detected according to the method in determination of fatty acid in food (GB 5009.168-2016), and the specific results are as follows:
table 2 summary of fatty acid content measurement results
Polyunsaturated fatty acids are essential for maintaining normal growth and development of the infant, particularly for promoting the growth and development of the central nervous system of the infant. Linoleic acid is a precursor form of arachidonic acid, can be converted into a plurality of bioactive molecules, is an acid metabolized into prostaglandin and other eicosanoid substance molecules, can promote the brain and eyesight development of infants, can also promote the metabolism of the infants and can improve the immunity. The alpha-linolenic acid exists in DHA and retina of human cranial nerves in large quantity, is extremely important for the development of infant brains, can promote the synthesis of nucleic acid proteins and monoamine neurotransmitters in the brains, and has important effects on the formation, growth, proliferation, differentiation and maturation of cerebral neurons, glial cells and nerve conduction synapses. The alpha-linolenic acid can enhance the permeability of cerebral nerve membranes and presynaptic and postsynaptic membranes, smooth nerve information transmission path, improve nerve reflex capacity, and further enhance the thinking ability, memory ability and stress ability of people.
As can be seen from Table 2, the infant formula milk powder for promoting intestinal health provided by the invention contains fatty acids with a proportion closer to that of breast milk, is more suitable for infants, and is superior to the traditional formula milk powder in paragraph 1 and the traditional formula goat milk powder in paragraph 1. Among them, the infant formula N1 for promoting intestinal health prepared in example 1 is closest to breast milk.
Example 8 Effect of infant formula for promoting intestinal health on intestinal health
One) in vitro proliferation effect of HMOs on Bifidobacterium animalis subsp lactis i797 with different proportions
The 2' -fucosyllactose, the lacto-N-tetraose and the lacto-N-neotetraose in the examples 1 to 6 were mixed in proportion, and the obtained HMOs1 to HMOs6 were added to the MRS medium in a one-to-one correspondence manner at a mass concentration of 0.2%, thereby obtaining experimental MRS media 1 to 6.
The experiment is divided into a control group and experiment groups 11-16, wherein the control group is prepared by 1% (10%) of activated bifidobacterium animalis subsp lactis i7976CFU/mL) in MRS culture medium without any other substances, and experimental groups 11-16 are obtained by inoculating 1% (10%) of activated Bifidobacterium animalis subsp lactis i797 in an experimental group6CFU/mL) is inoculated in experimental MRS culture media 1-6 correspondingly, and after anaerobic culture is carried out for 24 hours at 37 ℃ on a control group and experimental groups 11-16, the OD value of each group of culture solution is measured at the wavelength of 600 nm.
As shown in FIG. 1, the OD values of the cultured Bifidobacterium animalis L797 in the experimental groups 11-16 were significantly higher than those of the control group. The above experiments prove that 2 '-fucosyllactose, lacto-N-tetraose and lacto-N-neotetraose can promote the growth of bifidobacterium animalis subsp lactis i797, wherein the ratio of the 2' -fucosyllactose, lacto-N-tetraose and lacto-N-neotetraose in example 1 has better effect on the growth of bifidobacterium animalis subsp lactis i 797.
Second) Effect of infant formula promoting intestinal health on mouse intestinal tract
The control formula was prepared according to the method and amount of example 1, except that 2' -fucosyllactose, lacto-N-tetraose, lacto-N-neotetraose and bifidobacterium animalis subsp lactis i797 powder were not added to the control formula D1.
Selecting 42C 57BL/6 mice, randomly dividing the mice into 7 groups, wherein each group comprises 6 mice, namely a control group and 21-26 experimental groups, wherein the control group is intragastrically infused with 0.2mL of control formula milk powder (prepared by the infusion method in example 7) in the morning every day, the experimental groups 21-26 are intragastrically infused with 0.2mL of infant formula milk powder N1-N6 (prepared by the infusion method in example 7) for promoting intestinal health, the infant formula milk powder is infused with 0.2mL of infant formula milk powder for promoting intestinal health, the infusion is continued for 2 weeks, and feces are taken before the beginning and after the end of the intragastrically infused experiment for sIgA, pH and fecal flora detection and analysis. Wherein, the fecal flora detection analysis comprises the following steps: a) coating the feces on LBS culture medium, performing aerobic culture at 37 ℃ for 48h, and calculating the viable count of lactobacillus; b) coating the feces on a BBL agar culture medium, performing anaerobic culture at 37 ℃ for 48h, and calculating the viable count of the bifidobacteria; c) coating the feces on GAM culture medium, performing anaerobic culture at 37 deg.C for 48 hr, and calculating viable count of Bacteroides; d) and (3) coating the excrement on eosin methylene blue agar, carrying out aerobic culture at 37 ℃ for 48 hours, and calculating the viable count of the enterobacteria.
sIgA is an important immune substance and especially has important significance for maintaining the immune function of immature intestinal tracts of infants. The higher the sIgA concentration in the excrement is, the more positive the effects of enhancing immunity, preventing infection and promoting growth and development are.
As can be seen from FIG. 2, after the experiment is finished, the sIgA concentration in the feces of the mice in the control group and the experiment group of 21-26 is gradually increased compared with that before the experiment is started, which indicates that the intestinal tracts of the mice in the two groups are normally developed. However, at 14 days, the sIgA concentration in the feces of mice in experimental groups 21-26 was significantly higher than that in the control group, with the experimental group 1 being the most preferred. The experiments show that the 2' -fucosyllactose, the lactose-N-tetrasaccharide, the lactose-N-neotetrasaccharide and the bifidobacterium animalis subsp.
The pH value of the excrement is close to that of the colon, the pH value of the colon can be estimated by measuring the pH value of the excrement, and the reduction of the pH value of the colon is beneficial to inhibiting the growth and proliferation of saprophytic bacteria, so that intestinal diseases such as enteritis, colon cancer and the like are prevented/treated, and the intestinal health is promoted. Thus, a lower pH value of the stool indicates a healthier intestine.
As can be seen from FIG. 3, at the end of the experiment, the pH of the feces of the mice in the experimental groups 21-26 was significantly reduced compared to the control group, wherein the pH was optimal in the experimental group 1. The experiments show that the pH of intestinal tracts of suckling mice can be reduced by adding 2' -fucosyllactose, lactose-N-tetrasaccharide, lactose-N-neotetrasaccharide and bifidobacterium animalis subsp lactis i797 bacterial powder.
The flora content in the excrement can reflect the flora content in the intestinal tract, and the lactobacillus and the bifidobacterium are beneficial bacteria in the intestinal tract, and the more the lactobacillus and the bifidobacterium are, the healthier the intestinal tract is; bacteroides and Enterobacter are harmful intestinal bacteria, and the lower the number, the healthier the intestinal tract.
As can be seen from fig. 4, at the end of the experiment, compared with the control group, the numbers of useful intestinal bacteria lactobacillus and bifidobacterium of the suckling mice in the experimental groups 21-26 are obviously increased, and the numbers of two harmful bacteria bacteroides and enterobacter are obviously reduced, wherein the experimental group 1 is the best. The experiments show that the addition of 2' -fucosyllactose, lactose-N-tetraose, lactose-N-neotetraose and bifidobacterium animalis subsp lactis i797 bacterial powder can promote the proliferation of beneficial bacteria in intestinal tracts, and the composite use effect of the two is more obvious.
In conclusion, the infant formula milk powder for promoting intestinal health is added with the 2' -fucosyllactose, the lactose-N-tetraose, the lactose-N-neotetraose and the bifidobacterium animalis subsp lactis i797 bacterial powder in a reasonable proportion, so that the intestinal immunity can be enhanced, the proliferation of beneficial bacteria in the intestinal tract can be promoted, and the healthy development of the intestinal tract of an infant can be further promoted.
Claims (10)
1. An infant formula for promoting intestinal health, characterized in that,
the infant formula milk powder for promoting intestinal health contains bifidobacterium animalis subsp lactis i 797;
the effective components of the infant formula milk powder for promoting intestinal health comprise the following raw materials in parts by weight:
60-70 parts of cow milk/goat milk;
② nutritional agent: 9-12 parts of lactose, 8-10 parts of desalted whey powder, 6-8 parts of vegetable oil, 4-5 parts of 1, 3-dioleate-2-palmitic acid triglyceride, 1-3 parts of concentrated whey protein powder, 1-1.5 parts of 2' -fucosyllactose, 0.5-0.8 part of lactose-N-tetrasaccharide, 0.2-0.5 part of phospholipid, 0.15-0.3 part of lactose-N-neotetrasaccharide, 0.1-0.3 part of compound mineral substance, 0.1-0.15 part of compound vitamin, 0.05-0.1 part of calcium carbonate, 0.05-0.10 part of potassium hydroxide and 0.05-0.10 part of choline chloride;
③ nutrient enriching agent: 0.2-0.4 part of arachidonic acid powder, 0.1-0.2 part of fish oil powder and 0.01-0.05 part of nucleotide.
2. The infant formula for promoting intestinal health according to claim 1, wherein the raw materials for preparing the effective components of the infant formula for promoting intestinal health further comprise 0.01-0.05 parts of bifidobacterium animalis subsp.
3. The enteral health-promoting infant formula according to claim 1 or 2, wherein the content of Bifidobacterium animalis subsp9~1×1010CFU/g。
4. The infant formula for promoting intestinal health according to claim 1 or 2, wherein the vegetable oil is a mixture of soybean oil, rapeseed oil, sunflower oil and walnut oil.
5. The enteral infant formula according to claim 1 or 2, wherein the cow/goat milk is raw cow/goat milk, which is standardized and separated from impurities.
6. The preparation method of the infant formula milk powder for promoting the intestinal health, according to any one of claims 1 to 5, is characterized in that cow milk/goat milk is pasteurized, added with a nutrient and mixed uniformly, homogenized, sterilized, concentrated, spray-dried, then added with a nutrition enhancer and animal bifidobacterium lactis subsp.i 797 bacterial powder and mixed uniformly to obtain the infant formula milk powder for promoting the intestinal health.
7. The method for preparing infant formula for promoting intestinal health according to claim 6, wherein the pasteurization temperature is 60-70 ℃ for 15-18 s;
the sterilization temperature is 85-90 ℃ and the sterilization time is 22-30 s.
8. The method for preparing infant formula milk powder for promoting intestinal health according to claim 6 or 7, wherein the infant formula milk powder is further preheated to 60-65 ℃ after the nutritional agent is added and mixed uniformly and before homogenization; the homogenizing pressure is 16-18 MPa.
9. The method for preparing an infant formula for promoting intestinal health according to claim 6 or 7, wherein the concentration results in a density of 1090 to 1140kg/m3The concentrated milk of (1).
10. The method for preparing infant formula milk powder for promoting intestinal health according to claim 6 or 7, wherein the air inlet temperature of the spray drying is 165-180 ℃ and the air outlet temperature is 80-90 ℃.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202011411002 | 2020-12-04 | ||
CN2020114110021 | 2020-12-04 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN112790250A true CN112790250A (en) | 2021-05-14 |
Family
ID=75815005
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202110180540.2A Withdrawn CN112790250A (en) | 2020-12-04 | 2021-02-08 | Infant formula milk powder for promoting intestinal health and preparation method thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN112790250A (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113907144A (en) * | 2021-10-22 | 2022-01-11 | 内蒙古伊利实业集团股份有限公司 | Infant formula food added with HMO and application thereof |
CN114208893A (en) * | 2021-11-30 | 2022-03-22 | 内蒙古伊利实业集团股份有限公司 | Formula milk powder capable of improving intestinal microenvironment health and preparation method and application thereof |
CN114223723A (en) * | 2021-12-13 | 2022-03-25 | 黑龙江飞鹤乳业有限公司 | Nutritional composition, food product comprising the same and use of the nutritional composition |
CN114271340A (en) * | 2021-11-30 | 2022-04-05 | 内蒙古伊利实业集团股份有限公司 | Formula milk powder containing lactose-N-tetrasaccharide and preparation method and application thereof |
CN115918733A (en) * | 2022-11-30 | 2023-04-07 | 内蒙古伊利实业集团股份有限公司 | Formula milk powder and preparation method thereof |
CN116746683A (en) * | 2023-08-01 | 2023-09-15 | 河北源民生物科技有限公司 | Anti-dyspepsia flatulence and absorption promoting bifidobacterium animalis subspecies lactis BAL-28, fermentation product, flora CW and application |
-
2021
- 2021-02-08 CN CN202110180540.2A patent/CN112790250A/en not_active Withdrawn
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113907144A (en) * | 2021-10-22 | 2022-01-11 | 内蒙古伊利实业集团股份有限公司 | Infant formula food added with HMO and application thereof |
CN114208893A (en) * | 2021-11-30 | 2022-03-22 | 内蒙古伊利实业集团股份有限公司 | Formula milk powder capable of improving intestinal microenvironment health and preparation method and application thereof |
CN114271340A (en) * | 2021-11-30 | 2022-04-05 | 内蒙古伊利实业集团股份有限公司 | Formula milk powder containing lactose-N-tetrasaccharide and preparation method and application thereof |
CN114208893B (en) * | 2021-11-30 | 2023-11-10 | 内蒙古伊利实业集团股份有限公司 | Formula milk powder capable of improving intestinal microenvironment health and preparation method and application thereof |
CN114223723A (en) * | 2021-12-13 | 2022-03-25 | 黑龙江飞鹤乳业有限公司 | Nutritional composition, food product comprising the same and use of the nutritional composition |
CN115918733A (en) * | 2022-11-30 | 2023-04-07 | 内蒙古伊利实业集团股份有限公司 | Formula milk powder and preparation method thereof |
CN116746683A (en) * | 2023-08-01 | 2023-09-15 | 河北源民生物科技有限公司 | Anti-dyspepsia flatulence and absorption promoting bifidobacterium animalis subspecies lactis BAL-28, fermentation product, flora CW and application |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN112790250A (en) | Infant formula milk powder for promoting intestinal health and preparation method thereof | |
CN112868800A (en) | Infant formula milk powder containing breast milk oligosaccharide for improving immunity and preparation method thereof | |
CN108741083A (en) | Promote the neutral human milk oligosaccharides of beneficial bacteria growth | |
CN1838888A (en) | Infant or follow-on formula | |
AU2020294253B2 (en) | Nutritional Composition For Promoting Intestinal Health | |
CN103763940A (en) | Synbiotic combination of probiotic and human milk oligosaccharides to promote growth of beneficial microbiota | |
MX2010012905A (en) | Probiotics to improve gut microbiota. | |
CN112535218A (en) | Maternal emulsified infant formula milk powder for promoting intelligence development and intestinal health and preparation method and application thereof | |
CN109287920A (en) | A kind of solid beverage and preparation method thereof of multicomponent complex and multi-joint probiotics | |
CN103271274B (en) | Nutrient health-care porridge | |
CN111480849B (en) | Composite probiotic microcapsule powder for mother emulsification and preparation method and application thereof | |
US20240306690A1 (en) | Mixture of hmos and bifidobacteria | |
CN110679813A (en) | Probiotic health-care beverage for losing weight | |
TWI626893B (en) | Method for inhibiting pathogens using a nutritional composition | |
WO2018067002A1 (en) | Normalization of the intestinal microbiota composition in infants or toddlers fed with an amino acid-based nutritional composition | |
CN112075504A (en) | Infant formula milk powder for enhancing immunity | |
CN112167348A (en) | Formula milk powder for promoting growth and development of children and preparation method thereof | |
CN110692884A (en) | Probiotic health-care beverage assisting in reducing hypertension, hyperglycemia and hyperlipidemia | |
CN112535217A (en) | Maternal emulsified hypoallergenic fresh and alive infant formula milk and preparation method thereof | |
CN116286458B (en) | Lactobacillus plantarum LZ010 capable of reducing blood pressure and blood fat and application thereof | |
CN107467193A (en) | Full nutrition special medicine purposes formula food of a kind of pulvis based on goat milk and preparation method thereof | |
CN114223728B (en) | Premature/low birth weight infant formula containing prebiotics and probiotics | |
CN109601981A (en) | It is a kind of suitable for premature labor and nutrition spy's dietary seafood of low birth weight infant and preparation method thereof | |
CN111820284A (en) | Infant formula milk powder for optimizing amino acid pattern and improving immunity and preparation method thereof | |
CN112806577A (en) | Prebiotic probiotic synergistic combinations for butyric acid production |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
WW01 | Invention patent application withdrawn after publication |
Application publication date: 20210514 |
|
WW01 | Invention patent application withdrawn after publication |