CN112779119A - Small manual five-dimensional needle tip adjusting frame for cell experiment - Google Patents
Small manual five-dimensional needle tip adjusting frame for cell experiment Download PDFInfo
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- CN112779119A CN112779119A CN201911089286.4A CN201911089286A CN112779119A CN 112779119 A CN112779119 A CN 112779119A CN 201911089286 A CN201911089286 A CN 201911089286A CN 112779119 A CN112779119 A CN 112779119A
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- adjusting mechanism
- needle point
- needle
- transverse
- housing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
Abstract
The invention provides a small-sized manual five-dimensional needle tip adjusting frame for cell experiments. The invention comprises the following steps: the needle tip adjusting mechanism comprises a transverse and longitudinal shaft fine adjustment mechanism, the end portion of a needle is fixed behind the transverse and longitudinal shaft fine adjustment mechanism, and the needle tip is adjusted within a preset range through a transverse shaft key arranged on a transverse shaft of the needle tip adjusting mechanism and a longitudinal shaft key arranged on a longitudinal shaft of the needle tip adjusting mechanism. The invention effectively aligns the needle point to the cell to be punctured through five-dimensional adjustment, is accurate and effective, saves the experimental time, and has simple structure, simple and easy operation and convenient practical use for operators.
Description
Technical Field
The invention relates to the technical field of biological experiment equipment, in particular to a small-sized manual five-dimensional needle tip adjusting frame for cell experiments.
Background
With the progress of science and technology, scientific research and clinical application in the fields of biology, medicine and the like have gradually progressed to microcosmic and precise development, wherein cell micromanipulation is a technical support for promoting the development trend. The microscope is an experimental instrument widely applied in the field of biomedicine at present, in the existing cell related experiments, cells are often required to be punctured to extract cell sap and cytoplasm, and the technical level of cell micromanipulation directly determines the fineness and success rate of related scientific research and clinical application. Most of the existing puncture methods are that an experimenter holds the needle tip to move in person and searches for a puncture point under a microscope. However, the existing method is difficult to find a proper puncture point accurately and quickly, so that the experimental time is delayed, the experimental difficulty is increased, the experimental efficiency is low, and loss is possibly caused by fatigue or errors of operators.
The extraction of cells needs to be realized by relatively precise micro-adjustment, so that a five-dimensional adjusting frame with a fine adjustment structure and a portable structure needs to be developed to realize high-precision operation of the cells.
Disclosure of Invention
In accordance with the above-mentioned technical problem, a five-dimensional needle tip adjustment rack for a small-sized manual five-dimensional needle tip adjustment rack for cell experiments is provided. The invention mainly utilizes a frame capable of being adjusted in multiple dimensions, so that the needle point is gradually adjusted, and the cell puncture point is accurately found by matching with a microscope. The technical means adopted by the invention are as follows:
a small manual five-dimensional tip adjustment fixture for cell experiments, comprising: the needle point adjusting mechanism comprises a fixing part, a needle point position adjusting mechanism and a needle point posture adjusting mechanism, wherein the fixing part is provided with at least two threaded holes, the needle point position adjusting mechanism is provided with at least one threaded hole, the needle point posture adjusting mechanism comprises an adjusting screw rod, the fixing part is connected with the needle point position adjusting mechanism through the adjusting screw rod, so that the needle point position adjusting mechanism can change the pitching posture of the needle point based on the adjusting screw rod, the needle point position adjusting mechanism comprises a transverse and longitudinal axis fine adjusting mechanism, and the end part of a needle is fixed behind the transverse and longitudinal axis fine adjusting mechanism, the needle point is adjusted within a preset range through a transverse shaft key arranged on a transverse shaft of the needle point adjusting mechanism and a longitudinal shaft key arranged on a longitudinal shaft of the needle point adjusting mechanism.
Further, the main support mechanism comprises a housing, a rotating shaft is arranged outside the housing, and the transmission mechanism is specifically a sliding plate arranged inside the housing, and the sliding plate is connected with the rotating shaft.
Furthermore, the center of the transverse and longitudinal axis fine adjustment mechanism is provided with a fixing plate, and the end part of the needle is fixed on the fixing plate or is fixed on the fixing plate through a clamp.
Furthermore, the needle point position adjusting mechanism comprises a shell, a transverse and longitudinal axis fine adjusting mechanism and a fixed baffle, wherein the transverse and longitudinal axis fine adjusting mechanism is arranged inside the shell, and the fixed baffle is fixed on the shell on the side of the vertical supporting mechanism.
Furthermore, the fixing part is L-shaped, a ball is arranged at the corner of the L-shaped part, a fixing rod is arranged at the edge of the L-shaped part, one end of the spring is wound on the end part of the fixing rod, and the other end of the spring is fixed on the needle point position adjusting mechanism.
Further, horizontal axis of ordinates fine-tuning includes cross axle button, axis of ordinates button, fixed plate, still includes elastic compression part, cross axle button and axis of ordinates button all include button shell, the screw rod that links to each other with button shell, and the bottom and the fixed plate contact of screw rod still are equipped with the locking axle sleeve that is used for locking the screw rod between screw rod and the shell, elastic compression part sets up the opposite side for cross axle button, axis of ordinates button in the shell, elastic compression part is including connecting at the inboard spring of shell, the spring baffle who links to each other with the spring, and the opposite side and the fixed plate contact of spring baffle, and the fixed plate is smooth plane with screw rod, spring baffle's contact surface.
According to the invention, the lower connecting part of the vertical supporting mechanism is pushed and pulled by controlling the push rod, so that the adjustment in the horizontal direction is completed; the adjustment of the pitching and pitching directions of the needle point is completed by the propulsion of 2 adjusting screws; the needle tip is finely adjusted in a small range in a transverse and longitudinal mode through the transverse and longitudinal axis fine adjustment mechanism, and transverse and longitudinal adjustment is completed. Based on the five-dimensional adjustment, the needle point is effectively aligned to the cell to be punctured, the method is accurate and effective, and the experiment time is saved. Meanwhile, the cell micromanipulation experiment device is simple in structure, easy to operate, convenient for operators to use in practice and capable of effectively improving the success rate of cell micromanipulation experiments.
Based on the reasons, the invention can be widely popularized in the field of biological experimental equipment.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It is obvious that the drawings in the following description are some embodiments of the invention, and that for a person skilled in the art, other drawings can be derived from them without inventive exercise.
FIG. 1 is a schematic diagram of a five-dimensional needle tip adjustment frame according to an embodiment of the present invention.
FIG. 2 is a front view of the five-dimensional tip adjustment block without a sliding plate according to an embodiment of the present invention.
FIG. 3 is a left side view of a five-dimensional tip adjustment block in an embodiment of the present invention.
FIG. 4 is a right side view of a five-dimensional tip adjustment block in an embodiment of the present invention.
Fig. 5 is a bottom view of a five-dimensional needle tip adjustment holder according to an embodiment of the present invention.
Fig. 6 is a schematic diagram of an internal structure in an embodiment of the present invention.
In the figure: 1. a housing; 2. a rotating shaft; 3. a sliding plate; 4. a vertical support mechanism; 5. a fixed part; 6. adjusting the screw rod; 7. a needle; 8. a housing; 9. a longitudinal axis key; 10. a horizontal shaft key; 11. a screw; 12. fixing a baffle plate; 13. a spring I; 14. a fixing plate; 15. a spring baffle; 16. a spring II; 17. and a ball.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1 to 5, the present embodiment discloses a five-dimensional needle tip adjustment frame, which includes: the needle point adjusting mechanism comprises a fixing part 5, a needle point position adjusting mechanism and a needle point posture adjusting mechanism, wherein the fixing part 5 is provided with at least two threaded holes, the needle point position adjusting mechanism is provided with at least one threaded hole, the needle point posture adjusting mechanism comprises an adjusting screw rod 6, the fixing part 5 is connected with the needle point position adjusting mechanism through the adjusting screw rod 6, so that the needle point position adjusting mechanism can change the pitching posture of the needle point based on the adjusting screw rod 6, the needle point position adjusting mechanism comprises a transverse and longitudinal axis fine adjusting mechanism, and the end part of the needle is fixed behind the transverse and longitudinal axis fine adjusting mechanism, the needle 7 is adjusted within a predetermined range by a lateral axis button 10 provided on the lateral axis of the needle tip adjustment mechanism and a longitudinal axis button 9 provided on the longitudinal axis of the needle tip adjustment mechanism.
The main supporting mechanism comprises a shell 1, a rotating shaft 2 is arranged outside the shell 1, a transmission mechanism is changed according to different scenes of practical application, if the shell 1 is fixed left and right through a clamp, the rotating shaft 2 can be selected to be directly connected with a single screw or a screw-sliding plate 3-vertical supporting mechanism 4 or a structure of the screw-sliding plate 3-push rod-vertical supporting mechanism 4, the advancing displacement and the direction of the vertical supporting mechanism 4 are controlled by applying force to the rotating shaft 2, if the bottom of the shell 1 is fixed on the supporting mechanism, the sliding plate 3 connected with the shell 1 is arranged inside the shell 1, the other end of the sliding plate 3 is connected with the push rod, through holes are formed in the bottom of the shell 1, the two push rods bypass the through holes to ensure the safe use of the device, and meanwhile, a character-shaped sliding plate 3 and a character, L-shaped sliding plates 3, etc.
The central authorities of horizontal axis of ordinates fine-tuning are fixed plate 14, and the tip of needle is fixed on fixed plate 14 or the tip of needle passes through anchor clamps to be fixed on fixed plate 14, and the tip of needle is according to the difference of experiment, optional directly passes behind fixed plate 14, and is fixed through the nut of fixed plate 14 opposite side, if the less needle point of fixed size of fixed plate 14 can't fix, can be indirectly fixed through anchor clamps, anchor clamps can be cylindric, and its vertical recess of seting up the depth, after stretching into the recess with the tip of needle, with this cylindric anchor clamps joint on fixed plate 14.
The needle point position adjusting mechanism comprises a shell 8, a transverse and longitudinal shaft fine adjustment mechanism and a fixed baffle 12, the transverse and longitudinal shaft fine adjustment mechanism is arranged inside the shell 8, and the fixed baffle 12 is fixed on the shell 8 on the side of the vertical supporting mechanism 4.
The fixing part 5 is L-shaped, a ball 17 is arranged at the corner of the L-shaped part, a fixing rod is arranged at the edge of the L-shaped part, one end of a spring II 16 is wound at the end part of the fixing rod, and the other end of the spring II is fixed on the needle point position adjusting mechanism. The adjusting screw 6 is screwed into the shell 8 of the needle point position adjusting mechanism from the fixing part 5, in the using process, a certain adjusting screw 6 is pushed to enable the corresponding point of the needle point position adjusting mechanism to incline forwards, other points incline backwards to complete the pitching adjustment of the needle point, and the spring II 16 not only plays a role in resetting, but also achieves the limiting purpose through the traction of the fixing rod.
As shown in fig. 6, the transverse and longitudinal axis fine adjustment mechanism includes the transverse axis key 10, the longitudinal axis key 9, a fixing plate 14, and an elastic compression component, where the transverse axis key 10 and the longitudinal axis key 9 both include a key housing 1 and a screw rod 11 connected to the key housing 1, the bottom end of the screw rod contacts with the fixing plate 14, a locking shaft sleeve for locking the screw rod is further disposed between the screw rod and the housing 8, the locking shaft sleeve can be locked in a sliding manner, that is, a notch is formed in the shaft sleeve, a slider is disposed in the notch, the slider is drawn out, the screw rod can advance, and after the position of the screw rod is determined, the slider is pushed into the shaft. The elastic compression component is arranged on the other side of the shell 8 relative to the transverse axis key 10 and the longitudinal axis key 9 and comprises a spring I13 connected to the inner side of the shell 1 and a spring baffle 15 connected with the spring I13, the other side of the spring baffle 15 is in contact with a fixing plate 14, and the contact surfaces of the fixing plate 14, the screw and the spring baffle 15 are smooth planes.
Specifically, in the practical application process, the needle tip adjusting mechanism can be arranged on a desktop, an optical flat plate or other devices or can be held by hands, firstly, the screw rod is pushed through the rotating shaft 2, so that the needle tip adjusting mechanism approaches to a sample to be detected, after the front and back adjustment of the needle tip to the experimental body to be detected is finished, whether the positions of the horizontal axis and the longitudinal axis of the needle tip adjusting mechanism need to be adjusted is confirmed, if yes, the needle tip adjusting mechanism is gradually adjusted to a position suitable for the needle tip to puncture through the horizontal axis key 10 and the longitudinal axis key 9, and finally, the posture of the needle tip is adjusted through pushing. The embodiment can also improve the needle point, so that the cytoplasm which punctures the cell is sucked into the needle point through the siphon effect after the needle point is electrified, and a foundation is laid for the subsequent experiment.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (6)
1. A small manual five-dimensional needle tip adjustment frame for cell experiments is characterized by comprising: the needle point adjusting mechanism comprises a fixing part, a needle point position adjusting mechanism and a needle point posture adjusting mechanism, wherein the fixing part is provided with at least two threaded holes, the needle point position adjusting mechanism is provided with at least one threaded hole, the needle point posture adjusting mechanism comprises an adjusting screw rod, the fixing part is connected with the needle point position adjusting mechanism through the adjusting screw rod, so that the needle point position adjusting mechanism can change the pitching posture of the needle point based on the adjusting screw rod, the needle point position adjusting mechanism comprises a transverse and longitudinal axis fine adjusting mechanism, and the end part of a needle is fixed behind the transverse and longitudinal axis fine adjusting mechanism, the needle point is adjusted within a preset range through a transverse shaft key arranged on a transverse shaft of the needle point adjusting mechanism and a longitudinal shaft key arranged on a longitudinal shaft of the needle point adjusting mechanism.
2. The small-sized manual five-dimensional needle tip adjustment frame for cell experiments according to claim 1, wherein the main support mechanism comprises a housing, a rotating shaft is disposed outside the housing, the transmission mechanism is a sliding plate disposed inside the housing, and the sliding plate is connected with the rotating shaft.
3. The small-sized manual five-dimensional needle tip adjustment frame for cell experiments according to claim 1, wherein the center of the longitudinal and transverse axis fine adjustment mechanism is a fixing plate, and the end of the needle is fixed on the fixing plate or fixed on the fixing plate through a clamp.
4. The small-sized manual five-dimensional needle tip adjustment frame for cell experiments according to claim 3, wherein the needle tip position adjustment mechanism comprises a housing, a horizontal and vertical axis fine adjustment mechanism and a fixed baffle, the horizontal and vertical axis fine adjustment mechanism is arranged in the housing, and the fixed baffle is fixed on the housing on the side of the vertical support mechanism.
5. The small-sized manual five-dimensional needle tip adjustment frame for cell experiments according to claim 1, wherein the fixing part is L-shaped, a ball is disposed at a corner of the L-shaped, a fixing rod is disposed at an edge of the L-shaped, one end of the spring is wound around an end of the fixing rod, and the other end of the spring is fixed on the needle tip position adjustment mechanism.
6. The small-sized manual five-dimensional needle point adjusting rack for cell experiments according to any one of claims 1 to 5, wherein the transverse and longitudinal axis fine adjustment mechanism comprises a transverse axis button, a longitudinal axis button, a fixing plate, and an elastic compression component, the transverse axis button and the longitudinal axis button each comprise a button housing and a screw rod connected with the button housing, the bottom end of the screw rod is in contact with the fixing plate, a locking shaft sleeve for locking the screw rod is further arranged between the screw rod and the housing, the elastic compression component is arranged on the other side of the housing opposite to the transverse axis button and the longitudinal axis button, the elastic compression component comprises a spring connected to the inner side of the housing and a spring baffle connected with the spring, the other side of the spring baffle is in contact with the fixing plate, and the contact surfaces of the fixing plate, the screw rod and the.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911089286.4A CN112779119A (en) | 2019-11-08 | 2019-11-08 | Small manual five-dimensional needle tip adjusting frame for cell experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911089286.4A CN112779119A (en) | 2019-11-08 | 2019-11-08 | Small manual five-dimensional needle tip adjusting frame for cell experiment |
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| Publication Number | Publication Date |
|---|---|
| CN112779119A true CN112779119A (en) | 2021-05-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911089286.4A Pending CN112779119A (en) | 2019-11-08 | 2019-11-08 | Small manual five-dimensional needle tip adjusting frame for cell experiment |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110262891A1 (en) * | 2009-01-09 | 2011-10-27 | Ntn Corporation | Microinjection apparatus and microinjection method |
| CN105242076A (en) * | 2015-10-27 | 2016-01-13 | 华中科技大学 | Five-free-degree white light interference atomic power probe pose adjustment mechanism |
| CN106459860A (en) * | 2014-05-12 | 2017-02-22 | 北里科技有限公司 | Blade-mounted micropipette holding device and method for injecting sperm into ovular cytoplasm |
| CN206223597U (en) * | 2016-11-30 | 2017-06-06 | 山西省交通科学研究院 | It is a kind of for being accurately positioned the needle penetration test device that needle point is contacted with specimen surface |
| CN108070618A (en) * | 2017-12-12 | 2018-05-25 | 浙江海洋大学 | A kind of method of microinjection operation |
-
2019
- 2019-11-08 CN CN201911089286.4A patent/CN112779119A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110262891A1 (en) * | 2009-01-09 | 2011-10-27 | Ntn Corporation | Microinjection apparatus and microinjection method |
| CN106459860A (en) * | 2014-05-12 | 2017-02-22 | 北里科技有限公司 | Blade-mounted micropipette holding device and method for injecting sperm into ovular cytoplasm |
| CN105242076A (en) * | 2015-10-27 | 2016-01-13 | 华中科技大学 | Five-free-degree white light interference atomic power probe pose adjustment mechanism |
| CN206223597U (en) * | 2016-11-30 | 2017-06-06 | 山西省交通科学研究院 | It is a kind of for being accurately positioned the needle penetration test device that needle point is contacted with specimen surface |
| CN108070618A (en) * | 2017-12-12 | 2018-05-25 | 浙江海洋大学 | A kind of method of microinjection operation |
Non-Patent Citations (1)
| Title |
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| 李骏珉: "《鱼类遗传与育种学》", 31 December 1988, 中国林业出版社 * |
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Application publication date: 20210511 |
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