CN112778290B - Addition salt of S1P1 receptor agonist, crystal form and pharmaceutical composition thereof - Google Patents
Addition salt of S1P1 receptor agonist, crystal form and pharmaceutical composition thereof Download PDFInfo
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- CN112778290B CN112778290B CN202110053078.XA CN202110053078A CN112778290B CN 112778290 B CN112778290 B CN 112778290B CN 202110053078 A CN202110053078 A CN 202110053078A CN 112778290 B CN112778290 B CN 112778290B
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Abstract
The invention discloses a salt form and a crystal form of 1- { 2-fluoro-4- [5- (4-isobutylphenyl) -1,2,4-oxadiazole-3-yl ] -benzyl } -3-azetidinecarboxylic acid serving as a medicament for S1P1 receptor mediated diseases or disorders. The invention also discloses a preparation method of the salt form or the crystal form thereof, a pharmaceutical composition thereof and application thereof in preparing a medicament for treating and/or preventing diseases or symptoms mediated by S1P1 receptors.
Description
Technical Field
The application belongs to the technical field of pharmaceutical chemistry preparation and crystallization. In particular to a salt form and a crystal form of a medicament for diseases or symptoms mediated by S1P1 receptors, and also relates to a preparation method, a pharmaceutical composition and application of the salt form or the crystal form.
Background
1- { 2-fluoro-4- [5- (4-isobutylphenyl) -1,2,4-oxadiazol-3-yl]-benzyl } -3-azetidinecarboxylic acid of the formula C 23 H 24 FN 3 O 3 Molecular weight 409.45, chemical structure is shown in formula a below.
As used herein, the term "1- { 2-fluoro-4- [5- (4-isobutylphenyl) -1,2,4-oxadiazol-3-yl ] -benzyl } -3-azetidinecarboxylic acid" is used interchangeably with "the compound of formula A".
The compound shown in the formula A has S1P1 receptor agonist activity and selective specificity, and has remarkably shortened in vivo half-life, so that the compound is a high-quality second-generation S1P1 receptor agonist. Numerous studies have shown that there are a variety of S1P1 receptor agonists that are capable of binding to cognate receptors expressed on lymphocytes and leading to internalization of S1P1 receptors, which in turn prevents lymphocyte export. Therefore, S1P1 receptor agonists can reduce the ability of the body to initiate an immune response by preventing lymphocyte trafficking, and thus can be used as immunosuppressive agents for various autoimmune diseases.
In theory, the compound of formula A may be combined with one or more compounds of formula X m H n Wherein H is a dissociable hydrogen ion, X is a pharmaceutically acceptable anion, and m and n are natural numbers; the compounds of formula a may also be salified with one or more pharmaceutically acceptable cations, such as alkali metal ions or other pharmaceutically acceptable organic cations.
The identification, preparation, composition and use of compounds of formula a are disclosed in patent document CN103450171a, which is incorporated herein by reference. In particular, a method for preparing such a compound is disclosed in example 2. Patent document CN105315266a, which is incorporated by reference into the present application, discloses 12 crystalline forms of the compound shown in formula a. The inventor researches and discovers that the free bases have extremely low water solubility, the solubility in water at 25 ℃ is 1.1 mu g/mL, and the free bases present different stable forms in different solvent environments, for example, the most stable crystal form in water is crystal form I, and the most stable crystal form in organic solvents is crystal form IV. The limitations of the compound are therefore: they are both insoluble in water and show polymorphism in the free base form. Therefore, the research on the salt form of the compound shown in the formula A has very important practical significance, and certain undesirable physicochemical or biological pharmaceutical properties of the medicine can be improved by salifying, such as changing the solubility or dissolution rate, polymorphism and the like of the medicine.
Disclosure of Invention
In view of the deficiencies of the prior art, it is an object of the present invention to provide salt forms and crystalline forms of the compound of formula a having one or more improved properties, particularly with respect to polymorphism, solubility, crystalline stability, and chemical stability. For example, the salt forms of the compounds of formula a according to the present invention have one or more improved properties with respect to hygroscopicity, solubility, thermal stability (melting point and decomposition temperature) compared to other conventional salt forms, such as potassium, calcium, hydrochloride, citrate, phosphate.
The invention also aims to provide a preparation method of the salt form of the compound shown in the formula A, and the compound shown in the formula A has low solubility in most solvents and the solubility is not obviously improved at temperature, so that the salt form is difficult to be formed by using a conventional solution-solution mixed reaction mode. The preparation method of the salt form adopts various modes of mixed reaction of suspension-solution, solid-solvent, suspension-suspension and solid-suspension and the like to form salt, adopts a crystal form detection method to monitor the salt forming completeness, and adopts ion chromatography to confirm the salt forming ratio. Compared with the conventional salt formation method, the salt preparation method of the compound shown in the formula A has good controllability in the aspect of salt formation of the low-solubility compound.
The invention also provides a salt form of the compound shown in the formula A and a pharmaceutical composition of the crystal form and application of the salt form.
In accordance with the purpose of the present invention, there is provided 1- { 2-fluoro-4- [5- (4-isobutylphenyl) -1,2,4-oxadiazol-3-yl ] -benzyl } -3-azetidinecarboxylic acid sodium salt which is a compound of formula A and sodium ions formed at a 1:1 molar ratio, having the structure shown below:
as used herein, the term "1- { 2-fluoro-4- [5- (4-isobutylphenyl) -1,2,4-oxadiazol-3-yl ] -benzyl } -3-azetidinecarboxylic acid sodium salt" is used interchangeably with "sodium salt of a compound of formula A".
The sodium salt of the compound of formula a of the present invention is substantially crystalline, and preferably is an anhydride, hydrate or non-solvate. More preferably, according to the object of the present invention, the present invention provides a crystalline form of the sodium salt of the compound represented by formula a having an X-ray powder diffraction pattern expressed in terms of 2 θ angle having characteristic peaks of 4.4 ± 0.2 °, 6.6 ± 0.2 °, 14.7 ± 0.2 ° and 17.2 ± 0.2 ° at the following positions.
Further preferably, the present invention provides a crystalline form of the sodium salt of the compound represented by formula a, which form has an X-ray powder diffraction pattern expressed in terms of 2 θ angle with characteristic peaks and relative intensities thereof at the following positions:
without limitation, a typical example of the crystalline form of the sodium salt of the compound of formula a has an X-ray powder diffraction (XRPD) pattern as shown in figure 2. More preferably, the Fourier transform infrared spectrum of the crystal form of the sodium salt of the compound shown in the formula A is 1560cm in wave number -1 、1505cm -1 、1476cm -1 、1417cm -1 、1365cm -1 、1276cm -1 、885cm -1 、849cm -1 And 756cm -1 Has a characteristic peak.
According to the object of the present invention, the present invention provides a preparation method of a sodium salt of the compound represented by formula a or a crystal form thereof, the preparation method comprising the steps of: mixing a compound shown as a formula A with a molar ratio of 1:1-1:5 and sodium hydroxide in a solvent selected from alcohol, ketone, ether, water, nitrile or a mixture thereof, reacting, removing the solvent after the reaction is finished, and drying.
According to the embodiments of the present invention, for the preparation of the salt form, in the operation of removing the solvent after the completion of the reaction, a part of the solvent may be removed first, cooled, and then centrifuged, and the obtained solid may be dried; or removing all the solvent after the reaction is finished, adding the solvent into the solid again, pulping, centrifuging, and drying the obtained solid.
According to an embodiment of the present invention, for the preparation of the crystalline form, in the operation of removing the solvent after the completion of the reaction, a part of the solvent may be removed, a solid may be precipitated after cooling (e.g., to room temperature), and the obtained solid may be dried.
Preferably, the solvent is selected from methanol, ethanol, acetone, diethyl ether, water, acetonitrile or mixtures thereof.
Preferably, the molar ratio of the compound represented by the formula A to sodium hydroxide is 1.0-1.
Preferably, the reaction is carried out at 10 to 60 ℃, more preferably at room temperature; preferably, the reaction is carried out under stirring for a period of time ranging from 1 to 48 hours, more preferably from 3 to 24 hours.
Preferably, the drying is carried out under vacuum, the temperature of the drying being between 10 and 60 ℃, more preferably between 10 and 40 ℃.
Preferably, the drying time is 1 to 48 hours, more preferably 1 to 24 hours.
Preferably, the mass-to-volume ratio of the compound represented by formula a to the solvent in the preparation method is 1mg.
The "removal of solvent" can be accomplished using techniques conventional in the art, such as filtration, volatilization, centrifugation, nitrogen blowing, or spin drying; preferably, the solvent is removed by nitrogen blowing, volatilization or filtration; preferably, the experimental temperature for "solvent removal" is 10 to 60 ℃.
The sodium salt of the compound shown in the formula A and the crystal form thereof have the following beneficial effects:
1) The polymorphism of the sodium salt of the compound shown in the formula A is not obvious.
2) The solubility of the sodium salt of the compound shown in the formula A in water at 25 ℃ is 10mg/mL, and compared with the known free state of the compound shown in the formula A, the solubility of the sodium salt of the compound shown in the formula A in water is obviously improved, and the compound has better bioavailability.
3) The solubility of the sodium salt of the compound shown in the formula A in water at 25 ℃ is 10mg/mL, and compared with conventional salt types such as calcium salt of the compound shown in the formula A, hydrochloride of the compound shown in the formula A, citrate of the compound shown in the formula A, phosphate of the compound shown in the formula A and the like, the solubility in water is obviously improved, and the bioavailability is better.
4) Compared with the free state of the compound shown in the formula A, the crystal form of the sodium salt of the compound shown in the formula A is stable in an aqueous system, so that the crystal form has better application value in wet granulation or suspension dosage forms.
5) The crystal form of the sodium salt of the compound shown in the formula A is placed for 4 months at room temperature under the condition that the relative humidity is 10-90%, and the appearance, XRPD and melting point of the crystal form are not changed. The sodium salt of the compound shown in the formula A and the crystal form thereof have good storage stability, and can better avoid the problems of quality, safety and stability of the active ingredients of the medicine and the preparation formulation containing the sodium salt of the compound shown in the formula A or the crystal form thereof, such as impurity crystal forms, solubility difference and the like in the processes of medicine manufacturing and/or storage and the like.
The invention also provides a pharmaceutical composition, which comprises the sodium salt of the compound shown in the formula A and/or the crystal form thereof and at least one optional pharmaceutically acceptable carrier or excipient.
The invention also provides application of the sodium salt of the compound shown in the formula A and/or the crystal form thereof in preparing a medicament for treating and/or preventing diseases or symptoms mediated by S1P1 receptors.
In accordance with the purpose of the present invention, the present invention provides 1- { 2-fluoro-4- [5- (4-isobutylphenyl) -1,2,4-oxadiazol-3-yl ] -benzyl } -3-azetidinecarboxylic acid sulfate salt which is a compound of formula A and sulfuric acid formed at a 2:1 molar ratio and has the structure shown below:
the term "1- { 2-fluoro-4- [5- (4-isobutylphenyl) -1,2,4-oxadiazol-3-yl ] -benzyl } -3-azetidinecarboxylic acid sulfate" is used interchangeably herein with "sulfate of a compound of formula A".
The sulfate salt of the compound represented by formula a of the present invention is substantially crystalline, and is preferably an anhydride, a hydrate or a non-solvate. More preferably, according to the object of the present invention, the present invention provides a crystalline form of the sulfate salt of the compound of formula a having characteristic peaks in the following positions in an X-ray powder diffraction pattern expressed in 2 θ degrees using Cu-ka radiation: 5.4 +/-0.2 degrees, 8.1 +/-0.2 degrees, 14.8 +/-0.2 degrees, 16.7 +/-0.2 degrees and 18.3 +/-0.2 degrees.
More preferably, the crystal form of the sulfate salt of the compound shown in the formula A has characteristic peaks in the following positions in an X-ray powder diffraction pattern expressed by 2 theta angles: 5.4 +/-0.2 degrees, 8.1 +/-0.2 degrees, 14.8 +/-0.2 degrees, 15.6 +/-0.2 degrees, 16.7 +/-0.2 degrees, 18.3 +/-0.2 degrees, 21.0 +/-0.2 degrees, 22.0 +/-0.2 degrees, 22.9 +/-0.2 degrees, 25.2 +/-0.2 degrees and 26.3 +/-0.2 degrees.
Further preferably, the present invention provides a crystalline form of the sulfate salt of the compound represented by formula a, which crystalline form has an X-ray powder diffraction pattern expressed in terms of 2 θ angle with characteristic peaks and relative intensities at the following positions:
without limitation, a typical example of the crystalline form of the sulfate salt of the compound of formula a has an X-ray powder diffraction (XRPD) pattern as shown in figure 6.
The Fourier infrared spectrum of the crystal form of the sulfate of the compound shown in the formula A at the wave number of 1733cm -1 、1438cm -1 、1346cm -1 、1230cm -1 、1184cm -1 、1109cm -1 、1063cm -1 、1009cm -1 、885cm -1 、854cm -1 And 758cm -1 Has characteristic peaks.
According to an object of the present invention, the present invention provides a method for preparing a sulfate salt of the compound represented by formula a or a crystalline form thereof, the method comprising the steps of: mixing and reacting a compound represented by formula A, wherein the molar ratio of the compound to sulfuric acid is 1.4-1.
According to an embodiment of the present invention, for the preparation of the salt form, in the operation of removing the solvent after the completion of the reaction, a part of the solvent may be removed, filtered or centrifuged, and the obtained solid may be dried; or removing all the solvent after the reaction is finished, optionally adding the solvent into the solid again, pulping, centrifuging, and drying the obtained solid.
According to the specific embodiment of the present invention, for the preparation of the crystalline form, in the operation of removing the solvent after the completion of the reaction, all the solvent may be removed first, water may be added thereto for ultrasonic treatment, and then centrifugation may be performed, and the obtained solid may be dried.
Preferably, the solvent is selected from methanol, ethanol, n-propanol, acetone, tetrahydrofuran, water, acetonitrile or mixtures thereof.
Preferably, the molar ratio of the compound represented by the formula A to sulfuric acid is 1.
Preferably, the reaction is carried out at-10 to 60 ℃, more preferably at 10 to 40 ℃; preferably, the reaction is carried out under stirring for a period of time ranging from 1 to 72 hours, more preferably from 1 to 24 hours.
Preferably, the temperature of the drying is 10 to 60 ℃, more preferably 10 to 40 ℃.
Preferably, the drying time is 1 to 48 hours, more preferably 1 to 24 hours.
Preferably, the mass-to-volume ratio of the compound represented by formula a to the solvent in the preparation method is 1mg to 50mg, more preferably 4 mg.
The "removal of solvent" can be accomplished using techniques conventional in the art, such as filtration, volatilization, centrifugation, nitrogen blowing, or spin drying; preferably, the solvent is removed by nitrogen blowing, centrifugation or filtration; preferably, the experimental temperature for "solvent removal" is 10 to 60 ℃.
The "sulfuric acid" is concentrated sulfuric acid with a concentration of 98% (weight percent) and is commercially available.
The sulfate of the compound shown in the formula A and the crystal form thereof have the following beneficial effects:
1) The polymorphic form of the sulfate salt of the compound shown as the formula A is not obvious.
2) The solubility of the sulfate of the compound shown in the formula A in water at 25 ℃ is 19 mu g/mL, and compared with the known free state of the compound shown in the formula A, the solubility of the sulfate in water is obviously improved, and the sulfate has better bioavailability.
3) The solubility of the sulfate of the compound shown in the formula A in water at 25 ℃ is 19 mu g/mL, and compared with conventional salt types such as calcium salt of the compound shown in the formula A, hydrochloride of the compound shown in the formula A, citrate of the compound shown in the formula A, phosphate of the compound shown in the formula A and the like, the solubility in water is obviously improved, and the bioavailability is better.
4) The weight of the sulfate of the compound shown in the formula A is increased by 0.7% within the relative humidity range of 20% -80%, and compared with the conventional salt types such as the potassium salt of the compound shown in the formula A, the calcium salt of the compound shown in the formula A, the hydrochloride of the compound shown in the formula A, the citrate of the compound shown in the formula A, the phosphate of the compound shown in the formula A and the like, the sulfate of the compound shown in the formula A has lower moisture absorption weight increase, so that the sulfate has better storage stability.
5) The crystal form of the sulfate of the compound shown in the formula A is stable in a water-containing system, so that the crystal form has a good application value in wet granulation or suspension dosage forms.
6) The crystal form of the sulfate of the compound shown as the formula A is kept for 1 month under the conditions of normal, high temperature (60 ℃) and acceleration (40-75% relative humidity), and the appearance, XRPD and melting point of the crystal form are not changed. The sulfate of the compound shown in the formula A and the crystal form thereof have good storage stability, and can better ensure the quality, safety and stability problems of the active ingredients of the medicine and the preparation formulation containing the sulfate of the compound shown in the formula A or the crystal form thereof, such as impurity crystal forms, solubility difference and the like in the processes of medicine manufacture and/or storage and the like.
The invention also provides a pharmaceutical composition comprising the sulfate salt of the compound shown in the formula A and/or the crystal form thereof and optionally at least one pharmaceutically acceptable carrier or excipient.
The invention also provides application of the sulfate and/or the crystal form of the compound shown in the formula A in preparation of a medicament for treating and/or preventing diseases or symptoms mediated by S1P1 receptors.
In accordance with the purpose of the present invention, the present invention provides 1- { 2-fluoro-4- [5- (4-isobutylphenyl) -1,2,4-oxadiazol-3-yl ] -benzyl } -3-azetidinecarboxylic acid maleate which is a compound of formula A and maleic acid formed at a 1:1 molar ratio and has the structure shown below:
the terms "1- { 2-fluoro-4- [5- (4-isobutylphenyl) -1,2,4-oxadiazol-3-yl ] -benzyl } -3-azetidinecarboxylic acid maleate" and "maleate salt of compound of formula a" are used interchangeably herein.
The maleate salt of the compound of formula a of the present invention is substantially crystalline, and is preferably an anhydrate, hydrate or non-solvate. More preferably, according to the object of the present invention, the present invention provides a crystalline form of the maleate salt of the compound of formula a having characteristic peaks in the following positions in the X-ray powder diffraction pattern expressed in 2 Θ angles using Cu-ka radiation: 10.6 +/-0.2 degrees, 16.3 +/-0.2 degrees, 19.5 +/-0.2 degrees, 21.5 +/-0.2 degrees and 26.9 +/-0.2 degrees.
More preferably, the crystal form of the maleate salt of the compound of formula a has an X-ray powder diffraction pattern expressed in terms of 2 Θ angles with characteristic peaks at the following positions: 7.0 +/-0.2 degrees, 10.6 +/-0.2 degrees, 13.6 +/-0.2 degrees, 16.3 +/-0.2 degrees, 19.5 +/-0.2 degrees, 20.1 +/-0.2 degrees, 21.5 +/-0.2 degrees, 24.5 +/-0.2 degrees and 26.9 +/-0.2 degrees.
Further preferably, the present invention provides a crystalline form of the maleate salt of the compound of formula a having characteristic peaks and relative intensities at the following positions in an X-ray powder diffraction pattern expressed in degrees 2 Θ:
without limitation, a typical example of the crystalline form of the maleate salt of the compound of formula a has an X-ray powder diffraction (XRPD) pattern as shown in figure 10.
The Fourier infrared spectrum of the crystal form of the maleate of the compound shown in the formula A at the wave number of 1734cm -1 、1574cm -1 、1485cm -1 、1439cm -1 、1364cm -1 、1346cm -1 、1080cm -1 、1003cm -1 、893cm -1 、871cm -1 、757cm -1 And 729cm -1 Has a characteristic peak.
According to the object of the present invention, the present invention provides a preparation method of the maleate salt of the compound represented by the formula a or the crystal form thereof, the preparation method comprising the steps of: in a solvent selected from alcohol, ketone, ether (including cyclic ether), ester, water or a mixture thereof, respectively forming a suspension or a solution of a compound shown in formula A with the molar ratio of 1:1-1:5 and maleic acid, mixing and reacting, removing the solvent after the reaction is finished, and drying.
Preferably, the solvent is selected from ethanol, acetone, diethyl ether, water, ethyl acetate, 1,4-dioxane or mixtures thereof.
Preferably, the molar ratio of the compound represented by the formula a to maleic acid is 1.0 to 1.
Preferably, the reaction is carried out at-10 to 60 ℃, more preferably at 10 to 40 ℃; preferably, the reaction is carried out under stirring for a period of 10 to 72 hours, more preferably 10 to 24 hours.
Preferably, the drying temperature is 10 to 60 ℃, more preferably 10 to 40 ℃.
Preferably, the drying time is 1 to 48 hours, more preferably 1 to 24 hours.
Preferably, the mass-to-volume ratio of the compound shown in formula A to the solvent in the preparation method is 1mg.
The maleate and the crystal form of the compound shown in the formula A have the following beneficial effects:
1) The polymorphism of the maleate salt of the compound shown in the formula A is not obvious.
2) The solubility of the maleate of the compound shown in the formula A in water at 25 ℃ is 16 mu g/mL, and compared with the known free state of the compound shown in the formula A, the solubility in water is obviously improved, and the compound has better bioavailability.
3) The solubility of the maleate of the compound shown in the formula A in water at 25 ℃ is 16 mu g/mL, and compared with conventional salt forms such as calcium salt of the compound shown in the formula A, hydrochloride of the compound shown in the formula A, citrate of the compound shown in the formula A, phosphate of the compound shown in the formula A and the like, the solubility in water is obviously improved, and the compound has better bioavailability.
4) The weight of the maleate of the compound shown in the formula A is 0.4% within the relative humidity range of 20% -80%, and compared with the conventional salt types such as the potassium salt of the compound shown in the formula A, the calcium salt of the compound shown in the formula A, the hydrochloride of the compound shown in the formula A, the citrate of the compound shown in the formula A, the phosphate of the compound shown in the formula A and the like, the maleate has lower moisture absorption weight gain, so that the maleate has better storage stability.
5) The crystal form of the maleate of the compound shown in the formula A is stable in an aqueous system, so that the maleate has a good application value in wet granulation or suspension dosage forms.
6) The crystal form of the maleate salt of the compound of formula a of the present invention has no change in appearance, XRPD, and melting point when left under conventional, high temperature (60 ℃) and accelerated (40-75% relative humidity) conditions for 1 month. The crystal form of the maleate of the compound shown in the formula A has good storage stability, and can better ensure the quality, safety and stability of the active pharmaceutical ingredient and the maleate containing the compound shown in the formula A or a preparation formulation containing the crystal form of the maleate in the processes of pharmaceutical manufacture and/or storage and the like, such as impurity crystal forms, solubility difference and the like.
The invention also provides a pharmaceutical composition, which comprises the maleate salt of the compound shown in the formula A and/or the crystal form thereof and at least one optional pharmaceutically acceptable carrier or excipient.
The invention also provides application of the maleate and/or the crystal form of the compound shown in the formula A in preparation of a medicament for treating and/or preventing diseases or symptoms mediated by S1P1 receptors.
In any of the methods of the present invention for preparing a sodium salt of a compound of formula a, a crystalline form of a sodium salt of a compound of formula a, a sulfate of a compound of formula a, a crystalline form of a sulfate of a compound of formula a, a maleate of a compound of formula a, and a crystalline form of a maleate of a compound of formula a:
unless otherwise noted, "room temperature" means a temperature of about 10 to 30 ℃.
The "cyclic ether" may be tetrahydrofuran, 1,4-dioxane, etc.
The stirring can be carried out by conventional methods in the art, such as magnetic stirring and mechanical stirring, and the stirring speed is 50-1800 rpm, preferably 300-900 rpm.
The "removal of solvent" can be carried out by methods conventional in the art, such as filtration, evaporation, centrifugation, nitrogen blowing or spin drying. The "filtration" is generally a suction filtration at room temperature at a pressure less than atmospheric pressure, preferably at a pressure less than 0.09MPa. The "rotary evaporation method" is generally rotary evaporation at a pressure less than atmospheric pressure, preferably at a pressure less than 0.09MPa; the nitrogen blowing is generally to blow nitrogen through a nitrogen blower, and utilize the rapid flow of the nitrogen to volatilize the liquid; the specific operation of the centrifugation is as follows: placing the sample to be separated into a centrifuge tube, for example, centrifuging at 6000 rpm, until all solids settle to the bottom of the centrifuge tube; the specific operation of the volatilization is as follows: the sample solutions were left to evaporate to dryness at different temperatures. The experimental temperature for the "removal of solvent" is preferably 10 to 60 ℃.
The "drying" can be carried out by a conventional technique in the art, such as drying at room temperature, drying by air blowing, or drying under reduced pressure. The pressure can be reduced or normal, and is preferably less than 0.09MPa. The drying apparatus and method are not limited and may be a fume hood, forced air oven, spray dryer, fluidized bed drying or vacuum oven; it may be carried out under reduced pressure or without reduced pressure, preferably at a pressure of less than 0.09MPa.
The "crystal form" in the invention refers to a unique ordered molecular arrangement or configuration in crystal lattice, which is confirmed by the representation of an X-ray powder diffraction pattern of the compound. It is well known to those skilled in the art that experimental errors therein depend on instrument conditions, sample preparation and sample purity. The 2 theta angles of the peaks in the XRD pattern will typically vary slightly from instrument to instrument and from sample to sample. The difference in peak angle may vary from sample to sample by 1 °,0.8 °,0.5 °,03 °,0.1 °, etc., depending on the instrument, and usually allows an error of ± 0.2 °, so the difference in peak angle cannot be used as a unique criterion. The relative intensities of the peaks may vary with the sample, sample preparation, and other experimental conditions, so the order of peak intensities cannot be considered the only or determining factor. The influence of experimental factors such as sample height can cause an overall shift in peak angle, and a certain shift is usually allowed. Thus, it will be understood by those skilled in the art that any crystalline form having characteristic peaks identical or similar to the X-ray powder diffraction pattern of the present invention falls within the scope of the present invention. "single crystal form" means a single crystal form as determined by X-ray powder diffraction.
The novel salt forms of the compound of formula a of the present invention are substantially pure, single, and substantially free of any other crystalline or amorphous form. "substantially pure" when used in reference to a novel form herein means that the novel form constitutes at least 80% by weight, more specifically at least 90% by weight, especially at least 95% by weight, and especially at least 99% by weight of the compounds present.
The starting material compound represented by formula a of the present invention can be prepared by a method of production described in patent document CN103450171 a.
Further, the present invention provides a pharmaceutical composition comprising a therapeutically and/or prophylactically effective amount of one or more of the salt forms of the invention and/or crystalline and amorphous forms thereof or salts prepared by the process of the invention and/or crystalline and amorphous forms thereof, and optionally at least one pharmaceutically acceptable carrier or excipient. The salt form and the crystal form of the compound shown in the formula A comprise a sodium salt of the compound shown in the formula A, a crystal form of a sodium salt of the compound shown in the formula A, a sulfate of the compound shown in the formula A, a crystal form of a sulfate of the compound shown in the formula A, a maleate of the compound shown in the formula A and a crystal form of a maleate of the compound shown in the formula A. In addition, the pharmaceutical composition may also comprise other pharmaceutically acceptable salts of the compound of formula a or crystalline forms of the salts or amorphous forms of the salts.
The pharmaceutical composition can be prepared into certain dosage forms, preferably oral administration, parenteral administration (including subcutaneous administration, intramuscular administration and intravenous administration), rectal administration, transdermal administration, buccal administration, nasal administration and other forms of dosage forms, including but not limited to solid dosage forms, liquid dosage forms, semi-liquid dosage forms, aerosols or suppositories and the like. For example, dosage forms suitable for oral administration include tablets, capsules, granules, powders, pills, powders, lozenges, syrups or suspensions; dosage forms suitable for parenteral administration include aqueous or non-aqueous solutions or emulsions; dosage forms suitable for rectal administration include suppositories with hydrophilic or hydrophobic carriers; dosage forms suitable for transdermal administration include ointments, creams; dosage forms suitable for nasal administration include aerosols, sprays. The above dosage forms may be adapted for rapid, delayed or modified release of the active ingredient, as desired.
The pharmaceutically acceptable carrier of the present invention includes solid carriers, specifically including but not limited to: diluents, such as starch, pregelatinized starch, lactose, powdered cellulose, microcrystalline cellulose, calcium hydrogen phosphate, tricalcium phosphate, mannitol, sorbitol, sugar, and the like; binders such as acacia, guar gum, gelatin, polyvinylpyrrolidone, hydroxypropyl cellulose, hydroxypropyl methylcellulose, polyethylene glycol, and the like; disintegrants, such as starch, sodium starch glycolate, pregelatinized starch, crospovidone, croscarmellose sodium, colloidal silicon dioxide, and the like; lubricants, such as stearic acid, magnesium stearate, zinc stearate, sodium benzoate, sodium acetate, and the like; glidants such as colloidal silicon dioxide and the like; complex-forming agents, such as various grades of cyclodextrins and resins; release rate controlling agents such as hydroxypropyl cellulose, hydroxymethyl cellulose, hydroxypropyl methylcellulose, ethyl cellulose, methyl methacrylate, waxes, and the like. The pharmaceutically acceptable carrier of the present invention also includes liquid carriers, specifically including but not limited to: aqueous, oily or alcoholic solutions such as sterile water, physiological saline solution, glucose solution, mannitol solution, vegetable oil, cod liver oil, ethanol, propanol, glycerol, etc. In addition, carriers such as polyethylene glycol and polypropylene glycol can be used. Other pharmaceutically acceptable carriers may also be optionally used depending on the dosage form, including, but not limited to, film forming agents, plasticizers, colorants, flavoring agents, viscosity modifiers, preservatives, antioxidants, osmotic agents, buffers, and the like. Each carrier must be acceptable, compatible with the other ingredients of the formulation and not injurious to the patient.
The pharmaceutical compositions may be prepared using methods well known to those skilled in the art. In preparing the pharmaceutical composition, the sodium salt of the compound represented by formula a, the crystal form of the sodium salt of the compound represented by formula a, the sulfate of the compound represented by formula a, the crystal form of the sulfate of the compound represented by formula a, the maleate of the compound represented by formula a, the crystal form of the maleate of the compound represented by formula a, or a combination thereof of the present invention is mixed with one or more pharmaceutically acceptable carriers, optionally, with one or more other pharmaceutically active ingredients. Solid formulations may be prepared by mixing, granulating and the like, and liquid or semi-liquid dosage forms may be prepared by mixing, dissolving, dispersing, emulsifying and the like.
Further, the present invention provides the use of the salt form of the present invention and/or crystalline forms and amorphous forms thereof or the salt form obtained by the preparation process of the present invention and/or crystalline forms and amorphous forms thereof for the preparation of a medicament for the treatment and/or prevention of a disease or disorder mediated by S1P1 receptors. Wherein the salt form and the crystal form and the amorphous form thereof comprise sodium salt of the compound shown in the formula A, crystal form of sodium salt of the compound shown in the formula A, sulfate of the compound shown in the formula A, crystal form of sulfate of the compound shown in the formula A, maleate of the compound shown in the formula A, crystal form of maleate of the compound shown in the formula A or combination thereof. The disease or disorder mediated by the S1P1 receptor is selected from rheumatoid arthritis, multiple sclerosis, inflammatory bowel disease, autoimmune diseases, chronic inflammatory diseases, asthma, inflammatory neuropathies, arthritis, transplantation, crohn' S disease, ulcerative colitis, lupus erythematosus, psoriasis, ischemia-reperfusion injury, solid tumors, diseases associated with angiogenesis, vascular diseases, pain disorders, acute viral diseases, inflammatory bowel disease, insulin and non-insulin dependent diabetes mellitus and other related immune diseases; preferably, the disease or condition is selected from multiple sclerosis, rheumatoid arthritis, inflammatory bowel disease and psoriasis.
Further, the present invention provides a method of treating and/or preventing a disease or disorder mediated by S1P1 receptors, comprising administering to a subject in need thereof a therapeutically and/or prophylactically effective amount of a salt of the present invention and/or a crystalline form thereof or a combination thereof or a pharmaceutical composition thereof, wherein the salt and crystalline forms and amorphous forms thereof comprise a sodium salt of the compound of formula a, a crystalline form of a sodium salt of the compound of formula a, a sulfate salt of the compound of formula a, a crystalline form of a sulfate salt of the compound of formula a, a maleate salt of the compound of formula a, a crystalline form of a maleate salt of the compound of formula a, or a combination thereof. The disease or disorder mediated by the S1P1 receptor is selected from rheumatoid arthritis, multiple sclerosis, inflammatory bowel disease, autoimmune diseases, chronic inflammatory diseases, asthma, inflammatory neuropathies, arthritis, transplantation, crohn' S disease, ulcerative colitis, lupus erythematosus, psoriasis, ischemia-reperfusion injury, solid tumors, diseases associated with angiogenesis, vascular diseases, pain disorders, acute viral diseases, inflammatory bowel disease, insulin and non-insulin dependent diabetes mellitus and other related immune diseases; preferably, the disease or condition is selected from multiple sclerosis, rheumatoid arthritis, inflammatory bowel disease and psoriasis. The subject includes, but is not limited to, a mammal. The crystalline forms and amorphous forms provided herein or combinations thereof or pharmaceutical compositions thereof may be co-administered with other therapies or therapeutic agents. Also, the dosage of a compound or pharmaceutical composition required to effect a therapeutic, prophylactic or palliative effect, etc., will generally depend on the particular compound administered, the patient, the particular disease or condition and its severity, route and frequency of administration, etc., and will need to be determined on a case-by-case basis by the attending physician.
Drawings
FIG. 1 is an IR spectrum of a sodium salt of the compound represented by the formula A of example 3 of the present invention.
Figure 2 is an XRPD pattern of the sodium salt of the compound of formula a of example 3 of the invention.
FIG. 3 is a TGA profile of the sodium salt of the compound of formula A of example 3 of the present invention.
FIG. 4 is a DSC of the sodium salt of the compound of formula A of example 3 of the present invention.
FIG. 5 is an IR spectrum of a sulfate salt of the compound of formula A of example 13 of the present invention.
Figure 6 is an XRPD pattern of the sulfate salt of compound of formula a of example 13 of the present invention.
Figure 7 is a TGA profile of the sulfate salt of the compound of formula a of example 13 of the present invention.
FIG. 8 is a DSC of the sulfate salt of the compound of formula A of example 13 of the present invention.
FIG. 9 is an IR spectrum of the maleate salt of the compound of formula A example 21 of the present invention.
Figure 10 is an XRPD pattern of the maleate salt of compound of formula a of example 21 of the invention.
Figure 11 is a TGA profile of the maleate salt of the compound of formula a of example 21 of the present invention.
FIG. 12 is a DSC of the maleate salt of the compound of formula A of example 21 of the present invention.
Detailed Description
The invention will be further understood by reference to the following examples, which are not intended to be limiting. The detection instrument and the method comprise the following steps:
x-ray powder diffraction (XPRD): the instrument was a Bruker D8 Advance diffractometer. The samples were tested at room temperature. The detection conditions were as follows, angle range: 3-40 ° 2 θ, step size: 0.02 ° 2 θ, speed: 0.2 sec/step.
Differential thermal analysis (DSC) data was collected from TA Instruments Q200 MDSC. The detection method comprises the following steps: taking 1-10 mg of sample, placing the sample in a closed small-hole aluminum crucible, and heating the sample to 250 ℃ from room temperature at a heating rate of 10 ℃/min under the protection of 40mL/min dry N2.
Thermogravimetric analysis (TGA) data was taken from TA Instruments Q500 TGA. The detection method comprises the following steps: 5-15 mg of sample is placed in a platinum crucible, and the sample is heated to 300 ℃ from room temperature under the protection of 40mL/min dry N2 at a heating rate of 10 ℃/min by adopting a sectional high-resolution detection mode.
Hydrogen spectrum data (C:) 1 HNMR) was taken from Bruker Avance II DMX 400MHz NMR spectrometer. 1-5 mg of sample was weighed and dissolved in a nuclear magnetic sample tube with about 0.5mL of deuterated reagent for detection.
Infrared spectroscopic analysis (IR) data were taken from Bruker Tensor 27, with both instrument control software and data analysis software being OPUS. An ATR device is generally adopted and is arranged at 600-4000 cm -1 Within the range, an infrared absorption spectrum is collected.
Dynamic moisture sorption analysis (DVS) data and isothermal sorption analysis data were collected from TA Instruments Q5000 TGA. The detection method comprises the following steps: 1-10 mg of the sample was placed in a platinum crucible and the weight change was measured during the change of the relative humidity from 20% to 80%.
HPLC solubility data were obtained from Agilent 1260 HPLC. The column was Poroshell 120EC-C18 (2.7 x 50mm,4.6 μm), the detection wavelength was 254nm, the column temperature was 40 ℃, the flow rate was 1.5mL/min, and the sample size was 5 μ L. A sample was dissolved in the mobile phase B and prepared to have a concentration of about 0.45mg/mL and subjected to HPLC detection by the following gradient method to determine the sample concentration.
Ion Chromatograph (IC) data were collected from Dionex ICS-900, both workstation and analysis software were Chromeleon Console, and ion content was measured using external standard method.
The sample dissolution was facilitated by the sonication in the example, the apparatus being an ultrasonic cleaner operating at 40kHz for 15 minutes.
Preparation example 1A compound shown as the formula APreparation of the substance
The compound of formula A can be prepared by the preparation method of example 2 of patent document CN 103450171A.
The method comprises the following specific steps: a solution of 2-fluoro-4- [5- (4-isobutylphenyl) -1,2,4-oxadiazol-3-yl ] -benzaldehyde (9.0g, 27.8mmol), 3-azetidinecarboxylic acid (2.8g, 27.8mmol) and acetic acid (10 mL) in methanol-tetrahydrofuran (200 mL/200 mL) was stirred at room temperature for 2 hours, a solution of sodium cyanoborohydride (10.3g, 163.5mmol) in methanol (600 mL) was added, stirring was continued at room temperature for 16 hours, filtration was carried out, the filter cake was washed with methanol (100 mL) and dried to give 2.0g of a white solid product.
1 H-NMR (400MHz, CD3OD) delta: 8.13 (d, J =8.4hz, 2h), 8.05 (m, 1H), 7.97 (m, 1H), 7.68 (t, J =8.0hz,7.6hz, 1h), 7.42 (d, J =8.4hz, 2h), 4.40 (s, 2H), 4.15 (m, 4H), 3.41 (m, 1H), 2.61 (d, J =7.2hz, 2h), 1.95 (m, 1H), 0.94 (d, J =7.2hz, 6H), a compound shown as formula a, i.e., 1- { 2-fluoro-4- [5- (4-isobutylphenyl) -1,2,4-oxadiazol-3-yl]-benzyl } -3-azetidinecarboxylic acid.
Preparation example 2Screening and preparation of salt form of compound shown as formula A
2.1 salt sieves
According to the structure of the compound shown in the formula A, 12 types of I acids and 3 types of I bases are selected for carrying out salt sieve experiments. The experimental set-up and results are given in table 1 below.
TABLE 1 salt Screen Experimental setup and results
2.2 preparation of partial salts
Acetone and water are used as reaction solvents, the free state of the compound shown in the formula A and the molar ratio of counter ions are selected to be 1.2, salt formation is carried out, and the salt formation ratio is detected by IC (integrated Circuit), so that the citrate of the compound shown in the formula A, the phosphate of the compound shown in the formula A, the hydrochloride of the compound shown in the formula A, the potassium salt of the compound shown in the formula A and the calcium salt of the compound shown in the formula A are prepared.
Example 1Preparation of sodium salt of Compound of formula A
Weighing 14.50mg of the compound shown in the formula A prepared in the preparation example 1, adding 0.5mL of methanol, stirring to form a suspension, dropwise adding a sodium hydroxide solution (1.75 mg of sodium hydroxide is added into 0.45mL of methanol) into the methanol suspension of the compound shown in the formula A, stirring at room temperature for about 10 minutes to form a clear solution, continuously stirring for 3 hours, blowing off the solvent to 0.2mL by using nitrogen at room temperature to obtain a colorless transparent clear solution, cooling to 5 ℃ to obtain a suspension, centrifuging, and vacuum-drying the solid at room temperature for 16 hours to obtain the sodium salt of the compound shown in the formula A.
IC characterization shows that the sodium salt of the compound shown in the formula A is formed by reacting the compound shown in the formula A with sodium ions in a molar ratio of 1:1.
Example 2Preparation of sodium salt of Compound of formula A
Weighing 40.71mg of the compound shown in the formula A prepared in the preparation example 1, adding 0.4mL of methanol, stirring to form a suspension, dropwise adding a sodium hydroxide solution (4.0 mg of sodium hydroxide is added into 2.8mL of methanol) into the methanol suspension of the compound shown in the formula A, stirring at room temperature for about 1 hour to form a clear solution, continuing stirring for 2 hours, filtering, volatilizing the filtrate at room temperature to remove the solvent to 0.2mL to obtain a suspension, centrifuging, and vacuum-drying the solid at room temperature for 24 hours to obtain the sodium salt of the compound shown in the formula A.
IC characterization shows that the sodium salt of the compound shown in the formula A is formed by reacting the compound shown in the formula A with sodium ions in a molar ratio of 1:1.
Example 3Preparation of sodium salt of Compound of formula A
Weighing 4.9mg of sodium hydroxide, adding 1.0mL of water for ultrasonic dissolution, dropwise adding the clear liquid into 40.7mg of the compound shown in the formula A prepared in the preparation example 1, stirring for 24 hours at room temperature, filtering, blowing off the solvent to 0.2mL by using nitrogen at the temperature of 60 ℃ in the filtrate to obtain a light yellow transparent clear liquid, cooling to room temperature to separate out a solid, centrifuging, and drying the solid for 1 hour at the temperature of 40 ℃ in vacuum to obtain the sodium salt of the compound shown in the formula A.
IC characterization shows that the sodium salt of the compound shown in the formula A is formed by reacting the compound shown in the formula A with sodium ions in a molar ratio of 1:1.
The IR spectrum of the sodium salt is shown in FIG. 1.
The XRD pattern of the sodium salt is shown in fig. 2.
The TGA profile of the sodium salt is shown in figure 3.
The DSC chart of the sodium salt is shown in FIG. 4.
Example 4Preparation of sodium salt of Compound of formula A
3.5mg of sodium hydroxide is weighed, added with 1.0mL of acetone-water (4:1) for ultrasonic clearing, the clear solution is dripped into 29.2mg of the compound shown in the formula A prepared in the preparation example 1, stirred for 16 hours at room temperature and filtered, and the filter cake is placed at 40 ℃ for vacuum drying for 1 hour to obtain the sodium salt of the compound shown in the formula A.
IC characterization shows that the sodium salt of the compound shown in the formula A is formed by reacting the compound shown in the formula A with sodium ions in a molar ratio of 1:1.
Example 5Preparation of sodium salt of Compound of formula A
Weighing 5.05mg of the compound shown in the formula A prepared in the preparation example 1, adding 0.2mL of diethyl ether-ethanol (4:1) into the compound shown in the formula A, stirring to form a suspension, dropwise adding a sodium hydroxide solution (0.65 mg of sodium hydroxide is added into 0.3mL of diethyl ether-ethanol (the volume ratio is 4:1)) into the suspension of the compound shown in the formula A, stirring at room temperature for 24 hours, filtering, volatilizing the solvent from the filtrate at 60 ℃, pulping the solid with 0.2mL of diethyl ether for 1 hour, centrifuging, placing the centrifuged solid at room temperature, and drying in vacuum for 19 hours to obtain the sodium salt of the compound shown in the formula A.
IC characterization shows that the sodium salt of the compound shown in the formula A is formed by reacting the compound shown in the formula A with sodium ions in a molar ratio of 1:1.
Example 6Preparation of sodium salt of Compound of formula A
8.02mg of the compound represented by the formula A prepared in preparation example 1 was weighed, 8.0mL of n-butanol, methyl tert-butyl ether (1:1) and 2.5mg of sodium hydroxide solid were added, the mixture was stirred at 60 ℃ for 1 hour and filtered, the filtrate was subjected to rotary evaporation at 60 ℃ to remove the solvent, the solid was slurried with 0.2mL of n-butanol, methyl tert-butyl ether (1:1) for 1 hour and centrifuged, and the centrifuged solid was placed at 40 ℃ for vacuum drying for 48 hours to obtain the sodium salt of the compound represented by the formula A of the present invention.
IC characterization shows that the sodium salt of the compound shown in the formula A is formed by reacting the compound shown in the formula A with sodium ions in a molar ratio of 1:1.
Example 7Preparation of sodium salt of Compound of formula A
45.01mg of the compound of formula A prepared in preparation example 1 was weighed, 0.9mL of butanone, n-propanol (2:1) and 19.5mg of sodium hydroxide solid were added, stirred at 60 ℃ for 48 hours and filtered, the filtrate was rotary evaporated at room temperature to remove the solvent, the solid was slurried with 0.2mL of butanone, n-propanol (2:1) for 1 hour and centrifuged, and the solid was placed at 60 ℃ for vacuum drying for 40 hours to obtain the sodium salt of the compound of formula A of the present invention.
IC characterization shows that the sodium salt of the compound shown in the formula A is formed by reacting the compound shown in the formula A with sodium ions in a molar ratio of 1:1.
Example 8Preparation of sodium salt of Compound of formula A
Weighing 4.69mg of sodium hydroxide, adding 1.0mL of water into the mixture, ultrasonically dissolving the mixture, dropwise adding the clear solution into 38.77mg of the compound shown in the formula A prepared in the preparation example 1, then adding 14.0mL of water, stirring for 16 hours, filtering, blowing off the solvent from the filtrate to 0.2mL by nitrogen at 50 ℃ to obtain a light yellow transparent clear solution, cooling to 5 ℃ to separate out a solid, centrifuging, and drying the solid for 24 hours in vacuum at 40 ℃ to obtain the sodium salt of the compound shown in the formula A.
IC characterization shows that the sodium salt of the compound shown in the formula A is formed by reacting the compound shown in the formula A with sodium ions in a molar ratio of 1:1.
Example 9Preparation of sodium salt of Compound of formula A
Weighing 6.15mg of the compound shown in the formula A prepared in the preparation example 1, adding 3.0mL of methanol, isopropyl ether (1:1) and 1.3mg of sodium hydroxide solid, stirring at 40 ℃ for 1 hour, filtering, performing rotary evaporation on the filtrate at 50 ℃ to remove the solvent, pulping the solid with 0.1mL of methanol, isopropyl ether (1:1) for 1 hour, centrifuging, and performing vacuum drying on the centrifuged solid at 25 ℃ for 24 hours to obtain the sodium salt of the compound shown in the formula A.
IC characterization shows that the sodium salt of the compound shown in the formula A is formed by reacting the compound shown in the formula A with sodium ions in a molar ratio of 1:1.
Example 10Preparation of sodium salt of Compound of formula A
Weighing 35.62mg of the compound shown in the formula A prepared in the preparation example 1, adding 1.2mL of acetonitrile and 8.7mg of sodium hydroxide solid, stirring at 35 ℃ for 3 hours, filtering, performing rotary evaporation on the filtrate at room temperature to remove the solvent to 0.2mL to obtain a colorless transparent clear liquid, cooling to 5 ℃ to separate out a solid, centrifuging, and placing the solid at 40 ℃ for vacuum drying for 30 hours to obtain the sodium salt of the compound shown in the formula A.
IC characterization shows that the sodium salt of the compound shown in the formula A is formed by reacting the compound shown in the formula A with sodium ions in a molar ratio of 1:1.
Example 11Preparation of sulfate salt of compound represented by formula A
Weighing 76.02mg of the compound shown in the formula A prepared in the preparation example 1, adding 5.2mL of methanol, stirring to form a suspension, dropwise adding a sulfuric acid solution (7.3 mg of 98% sulfuric acid is added into 7.6mL of methanol) into the methanol suspension of the compound shown in the formula A, stirring at room temperature for 5 hours to form a suspension, adding 5.0mL of methanol, continuing stirring for 1 hour, filtering, blowing off the solvent from the filtrate by using nitrogen at room temperature to 1.0mL to obtain a suspension, filtering, and vacuum-drying the solid at room temperature for 20 hours to obtain the sulfate of the compound shown in the formula A.
IC characterization shows that the sulfate of the compound shown in the formula A is formed by reacting the compound shown in the formula A with sulfuric acid in a molar ratio of 2:1.
Example 12Preparation of sulfate salt of compound represented by formula A
34.41mg of the compound represented by the formula A prepared in preparation example 1 was weighed, added to 1.0mL of ethanol and stirred to form a suspension, 24.82mg of 98% sulfuric acid was added to the ethanol suspension of the compound represented by the formula A, stirred at room temperature for 24 hours, filtered, and the filter cake was vacuum-dried at 40 ℃ for 10 hours to obtain a sulfate salt of the compound represented by the formula A of the present invention.
IC characterization shows that the sulfate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and sulfuric acid in a molar ratio of 2:1.
Example 13Preparation of sulfate salt of compound represented by formula A
Weighing 4.63mg of the compound shown in the formula A prepared in the preparation example 1, adding 0.2mL of n-propanol, stirring to form a suspension, dropwise adding a sulfuric acid solution (8.79 mg of 98% sulfuric acid is added into 0.3mL of n-propanol) into the n-propanol suspension of the compound shown in the formula A, stirring at room temperature for 16 hours, filtering, removing the solvent from the filtrate at room temperature by nitrogen blowing to obtain an oily substance, adding water into the oily substance, performing ultrasonic treatment to form a suspension, centrifuging, and performing vacuum drying on the solid at room temperature for 24 hours to obtain the sulfate of the compound shown in the formula A.
IC characterization shows that the sulfate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and sulfuric acid in a molar ratio of 2:1.
The IR spectrum of the sulfate salt is shown in FIG. 5.
The XRD pattern of the sulfate is shown in FIG. 6.
The TGA profile of the sulfate salt is shown in FIG. 7.
The DSC chart of the sulfate salt is shown in FIG. 8.
Example 14Preparation of sulfate salt of compound represented by formula A
10.02mg of the compound represented by the formula A prepared in preparation example 1 was weighed, added to 1.0mL of water and stirred to form a suspension, 7.88mg of 98% sulfuric acid was added to the aqueous suspension of the compound represented by the formula A, stirred at 40 ℃ for 24 hours and then filtered, and the filter cake was vacuum-dried at 60 ℃ for 1 hour to obtain a sulfate salt of the compound represented by the formula A of the present invention.
IC characterization shows that the sulfate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and sulfuric acid in a molar ratio of 2:1.
Example 15Preparation of sulfate salt of compound represented by formula A
34.4mg of the compound represented by the formula A prepared in preparation example 1 was weighed, added to 1.0mL of water and stirred to form a suspension, a sulfuric acid solution (25.0 mg of 98% sulfuric acid was added to 0.5 mL) was added dropwise to the aqueous suspension of the compound represented by the formula A, stirred at room temperature for 24 hours and then filtered, and the filter cake was vacuum-dried at 40 ℃ for 1 hour to obtain a sulfate salt of the compound represented by the formula A of the present invention.
IC characterization shows that the sulfate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and sulfuric acid in a molar ratio of 2:1.
Example 16Preparation of sulfate salt of compound represented by formula A
Weighing 10.25mg of the compound shown in the formula A prepared in the preparation example 1, adding 0.2mL of water, stirring to form a suspension, sequentially adding 8.25mg of 98% sulfuric acid and 1.0mL of acetone into the aqueous suspension of the compound shown in the formula A, stirring at room temperature for 1 hour to obtain a clear solution, filtering, removing the solvent from the filtrate by nitrogen blowing at room temperature, and drying the solid at room temperature for 24 hours in vacuum to obtain the sulfate of the compound shown in the formula A.
IC characterization shows that the sulfate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and sulfuric acid in a molar ratio of 2:1.
Example 17Preparation of sulfate salt of Compound represented by formula A
Weighing 10.40mg of the compound shown in the formula A prepared in the preparation example 1, sequentially adding 0.2mL of water, 7.92mg of 98% sulfuric acid and 1.0mL of tetrahydrofuran into the compound shown in the formula A, stirring at room temperature for 3 hours to obtain a clear solution, filtering, blowing off the solvent from the filtrate at 60 ℃ by nitrogen to 0.3mL to obtain a suspension, centrifuging, and drying the solid at 40 ℃ for 20 hours in vacuum to obtain the sulfate of the compound shown in the formula A.
IC characterization shows that the sulfate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and sulfuric acid in a molar ratio of 2:1.
Example 18Preparation of sulfate salt of compound represented by formula A
Weighing 4.15mg of the compound shown in the formula A prepared in the preparation example 1, adding 0.2mL of water acetonitrile (1:4) to stir to form a suspension, adding a sulfuric acid solution (3.2 mg of sulfuric acid with the concentration of 98% to 0.3mL of water acetonitrile (1:4)) dropwise to the water acetonitrile (1:4) suspension of the compound shown in the formula A, stirring at room temperature for 24 hours, filtering, removing the solvent from the filtrate at room temperature by nitrogen blowing to 0.1mL to obtain a suspension, centrifuging, and drying the solid at 50 ℃ in vacuum for 1 hour to obtain the sulfate of the compound shown in the formula A.
IC characterization shows that the sulfate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and sulfuric acid in a molar ratio of 2:1.
Example 19Preparation of sulfate salt of compound represented by formula A
Weighing 5.0mg of the compound shown in the formula A prepared in the preparation example 1, adding 5.0mL of sec-butyl alcohol, namely butanone (1:4) and 10.3mg of sulfuric acid with the concentration of 98%, stirring at 10 ℃ for 30 hours, filtering, removing the solvent from the filtrate at 40 ℃ by nitrogen blow to 0.1mL to obtain a suspension, centrifuging, and drying the solid at 60 ℃ in vacuum for 10 hours to obtain the sulfate of the compound shown in the formula A.
IC characterization shows that the sulfate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and sulfuric acid in a molar ratio of 2:1.
Example 20Preparation of sulfate salt of compound represented by formula A
40.0mg of the compound of the formula A prepared in preparation example 1 was weighed, added to 0.4mL1, 4-dioxane: water (1:1) and stirred to form a suspension, a sulfuric acid solution (96.7 mg of 98% sulfuric acid was added to 0.4mL1, 4-dioxane: water (1:1)) was added dropwise to a suspension of 1,4-dioxane: water (1:1) of the compound of the formula A, stirring was carried out at 60 ℃ for 72 hours and then filtration was carried out, the filtrate was purged with nitrogen at 60 ℃ to remove the solvent, the solid was beaten with 0.2mL of 1, 4-dioxane: water (1:1) for 1 hour and then centrifuged, and the solid was dried under vacuum at 40 ℃ for 48 hours after centrifugation to obtain the sulfate of the compound of the formula A of the present invention.
IC characterization shows that the sulfate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and sulfuric acid in a molar ratio of 2:1.
Example 21Preparation of maleate salt of compound of formula A
51.7mg of the compound represented by the formula A prepared in preparation example 1 was weighed, 1.0mL of acetone was added, a maleic acid solution (17.7 mg of maleic acid was added to 1.0mL of acetone) was added dropwise to the acetone system of the compound represented by the formula A with stirring, stirred at room temperature for 24 hours, filtered, and vacuum-dried at 40 ℃ for 16 hours to obtain the maleate salt of the compound represented by the formula A of the present invention.
IC characterization shows that the maleate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and maleic acid in a molar ratio of 1:1.
The maleate salt IR spectrum is shown in FIG. 9.
The XRD pattern of the maleate salt is shown in figure 10.
The TGA profile of the maleate salt is shown in figure 11.
The DSC chart of the maleate salt is shown in FIG. 12.
Example 22Preparation of maleate salt of compound shown as formula A
10.37mg of the compound represented by the formula A prepared in preparation example 1 was weighed, a maleic acid solution (3.91 mg of maleic acid was added to 1.0mL of ethanol) was added dropwise to the compound represented by the formula A, stirred at room temperature for 10 hours, filtered, and the filter cake was vacuum-dried at 25 ℃ for 20 hours to obtain a maleate salt of the compound represented by the formula A of the present invention.
IC characterization shows that the maleate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and maleic acid in a molar ratio of 1:1.
Example 23Preparation of maleate salt of compound shown as formula A
7.63mg of the compound represented by the formula A prepared in preparation example 1 was weighed, a maleic acid solution (4.47 mg of maleic acid was added to 1.0mL of water) was added dropwise to the compound represented by the formula A, stirred at 40 ℃ for 24 hours, filtered, and the filter cake was vacuum-dried at 40 ℃ for 1 hour to obtain a maleate salt of the compound represented by the formula A of the present invention.
IC characterization shows that the maleate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and maleic acid in a molar ratio of 1:1.
Example 24Preparation of maleate salt of compound shown as formula A
10.70mg of the compound represented by the formula A prepared in preparation example 1 was weighed, and 3.52mg of maleic acid and 1.0mL of diethyl ether were added to the compound represented by the formula A, followed by stirring at room temperature for 24 hours, filtration, and vacuum drying of the filter cake at 10 ℃ for 24 hours, to obtain the maleate salt of the compound represented by the formula A of the present invention.
IC characterization shows that the maleate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and maleic acid in a molar ratio of 1:1.
Example 25Preparation of maleate salt of compound shown as formula A
13.33mg of the compound represented by the formula A prepared in preparation example 1 was weighed, 1.5mL of ethyl acetate was added, a maleic acid solution (5.14 mg of maleic acid was added to 1.0mL of the ethyl acetate solution) was added dropwise to the ethyl acetate system of the compound represented by the formula A with stirring, and the mixture was stirred at room temperature for 18 hours, filtered, and the filter cake was vacuum-dried at 40 ℃ for 1 hour to obtain the maleate salt of the compound represented by the formula A of the present invention.
IC characterization shows that the maleate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and maleic acid in a molar ratio of 1:1.
Example 26Preparation of maleate salt of compound shown as formula A
6.04mg of the compound represented by the formula A prepared in preparation example 1 was weighed, 1.0mL of 1, 4-dioxane was added, a maleic acid solution (4.4 mg of maleic acid was added to 0.4mL of 1, 4-dioxane) was added dropwise to the 1,4-dioxane system of the compound represented by the formula A under stirring, and the mixture was stirred at room temperature for 20 hours, filtered, and the filter cake was vacuum-dried at 50 ℃ for 24 hours to obtain 34.3mg of the maleate salt of the compound represented by the formula A of the present invention.
IC characterization shows that the maleate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and maleic acid in a molar ratio of 1:1.
Example 27Preparation of maleate salt of compound shown as formula A
5.0mg of the compound represented by the formula A prepared in preparation example 1 was weighed, added with 4.7mg of maleic acid and 5.0mL of methyl butanone: methyl formate (2:1), stirred at 60 ℃ for 30 hours, filtered, and vacuum-dried at 56 ℃ for 37 hours to obtain the maleate salt of the compound represented by the formula A of the present invention.
IC characterization shows that the maleate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and maleic acid in a molar ratio of 1:1.
Example 28Preparation of maleate salt of compound shown as formula A
40.5mg of the compound represented by the formula A prepared in preparation example 1 was weighed, and 0.6mL of methanol was added: methyl tert-butyl ether (1:1), a solution of maleic acid (11.5 mg maleic acid added to 0.4mL methanol: methyl tert-butyl ether (1:1) solution) was added dropwise with stirring to a methanol of the compound of formula A: stirring for 48 hours at 45 ℃ in a system of methyl tert-butyl ether (1:1), filtering, and vacuum drying for 48 hours at 40 ℃ to obtain the maleate salt of the compound shown in the formula A.
IC characterization shows that the maleate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and maleic acid in a molar ratio of 1:1.
Example 29Preparation of maleate salt of compound shown as formula A
50.0mg of the compound represented by the formula A prepared in preparation example 1 was weighed, 0.5mL of n-butanol: isopropyl acetate (3:1) was added, and a maleic acid solution (70.9 mg of maleic acid was added to 0.5mL of n-butanol: isopropyl acetate (3:1) solution) was added dropwise to a system of n-butanol: isopropyl acetate (3:1) of the compound represented by the formula A under stirring at-10 ℃ for 72 hours, filtered, and vacuum-dried at 60 ℃ for 30 hours to obtain the maleate salt of the compound represented by the formula A of the present invention.
IC characterization shows that the maleate of the compound shown in the formula A is formed by reacting the compound shown in the formula A and maleic acid in a molar ratio of 1:1.
Comparative example 1Solubility of sodium salt of Compound of formula A
The sodium salt of the compound shown in the formula A is taken to carry out a solubility experiment in water, and the specific operation is as follows: 5mg of sodium salt of the compound shown in the formula A is taken in a 20ml glass bottle, and deionized water is gradually dripped into the glass bottle under the condition of 25 ℃ for ultrasonic treatment until the sodium salt is dissolved clearly. The solubility of the sample in water was calculated.
TABLE 2 solubility in water of the sodium salt of the compound of formula A according to the invention
| Sample name | Solubility (mg/mL) |
| A sodium salt of a compound of |
10 |
As can be seen from table 2, the sodium salt of the compound of formula a of the present invention has higher solubility and thus better bioavailability.
Comparative example 2Comparison of thermal stability of salt form of Compound represented by formula A
DSC and TGA analysis of the sodium salt of the compound of formula A of the present invention and conventional salts (citrate of the compound of formula A, phosphate of the compound of formula A, hydrochloride of the compound of formula A) were carried out to obtain melting point and decomposition temperature data of each salt form.
TABLE 3 melting Point data for sodium and other conventional salts of the inventive Compound of formula A
| Salt form | Melting Point (. Degree.C.) | Decomposition temperature (. Degree.C.) |
| A sodium salt of a compound of formula A | 234 | 275 |
| Citrate salts of compounds of formula A | 152 | 154 |
| Phosphate salts of compounds of formula A | 160 | 190 |
| Hydrochloride salt of a compound of formula A | 163 | 145 |
As can be seen from table 3, the sodium salt of the compound represented by formula a of the present invention has a very high melting point and decomposition temperature compared to conventional salts (citrate of the compound represented by formula a, phosphate of the compound represented by formula a, hydrochloride of the compound represented by formula a), and thus has better thermal stability.
Comparative example 3Solubility comparison of salt forms of Compounds of formula A
A water solubility test is carried out on the known free compound shown in the formula A, the conventional salt (calcium salt of the compound shown in the formula A, citrate of the compound shown in the formula A, phosphate of the compound shown in the formula A, hydrochloride of the compound shown in the formula A), the sulfate of the compound shown in the formula A and the maleate of the compound shown in the formula A, and the specific operation is as follows: taking 5mg of known free state and conventional salt (calcium salt of the compound shown in the formula A, citrate of the compound shown in the formula A, phosphate of the compound shown in the formula A and hydrochloride of the compound shown in the formula A), sulfate of the compound shown in the formula A and maleate of the compound shown in the formula A, which are prepared by the invention, into a 20mL glass bottle, adding 15mL of deionized water, and stirring at 25 ℃ for 2 hours. Sampling, filtering and detecting the concentration by HPLC. And calculating the solubility of the effective components in the sample in water.
TABLE 4 solubility in water of free compounds of formula A and their salts
| Sample name | Solubility (μ g/mL) |
| The compound shown as the formula A is in a free state | 1.1 |
| Sulfate salts of compounds of formula A | 19.2 |
| Maleate salt of a compound of formula A | 16.1 |
| Calcium salt of a compound of formula A | 2.5 |
| A citrate salt of a compound of formula A | 5.3 |
| Phosphate salts of compounds of formula A | 6.7 |
| Hydrochloride salt of a compound of formula A | 3.8 |
As can be seen from Table 4, the sulfate salt of the compound of formula A and the maleate salt of the compound of formula A according to the present invention have about 10 to 20 times higher solubility in water at 25 ℃ than the known free form of the compound of formula A; compared with other conventional salts (calcium salt of the compound shown in the formula A, citrate of the compound shown in the formula A, phosphate of the compound shown in the formula A and hydrochloride of the compound shown in the formula A), the solubility of the compound in water at 25 ℃ is improved by about 3-8 times, and the compound has better solubility, so that the compound has better bioavailability.
Comparative example 4Absorption of salt form of compound represented by formula AComparison of moisture Properties
DVS analysis was performed on sulfate of the compound represented by formula A and maleate of the compound represented by formula A according to the present invention, and conventional salts (potassium salt of the compound represented by formula A, calcium salt of the compound represented by formula A, citrate of the compound represented by formula A, phosphate of the compound represented by formula A, hydrochloride of the compound represented by formula A) were used to obtain hygroscopicity data of each salt form.
TABLE 5 hygroscopicity data for the sulfate salt of the compound of formula A of the present invention and the maleate salt of the compound of formula A with other conventional salts
| Salt form | Moisture absorption amount (%) | Appearance of the product |
| Sulfate salts of compounds of formula A | 0.7 | Powder of |
| A maleate salt of a compound of formula A | 0.4 | Powder of |
| Potassium salt of a compound of formula A | 17.5 | Deliquescing to form a solution |
| Calcium salt of a compound of formula A | 1.2 | Powder of |
| Citrate salts of compounds of formula A | 0.7 | Powder of |
| Phosphate salts of compounds of formula A | 1.2 | Powder of |
| Hydrochloride salt of a compound of formula A | 1.2 | Powder of |
As can be seen from table 5, the sulfate of the compound represented by formula a and the maleate of the compound represented by formula a according to the present invention have lower hygroscopic weight gain than conventional salts (potassium salt of the compound represented by formula a, calcium salt of the compound represented by formula a, citrate of the compound represented by formula a, phosphate of the compound represented by formula a, hydrochloride of the compound represented by formula a), and thus have better storage stability, and can better avoid quality, safety and stability problems during manufacturing and/or storage of the drug, etc.
Comparative example 5Comparison of stability of Crystal forms of salts of Compounds of formula A
Taking the crystal form of the sulfate of the compound shown in the formula A and the crystal form of the maleate of the compound shown in the formula A to perform stability experiments, the operation is as follows: the crystal form of the sulfate of the compound shown in the formula A and the crystal form of the maleate of the compound shown in the formula A, which are prepared by the method, 60mg samples are respectively placed under the conditions of convention (sealed and placed in a dark place at 25 ℃), high temperature (sealed and placed in a dark place at 60 ℃) and acceleration (open and placed in a dark place at 40-75% relative humidity) for 30 days, and the stability of the crystal forms is inspected.
TABLE 6 statistics of the results of the crystal form stability tests of the sulfate salt of the compound of formula A and the maleate salt of the compound of formula A of the present invention
As can be seen from Table 6, the crystal form of the sulfate of the compound shown in the formula A and the crystal form of the maleate of the compound shown in the formula A both have better stability, and are favorable for adapting to various environmental conditions of manufacture, storage and transportation.
Comparative example 6Comparison of stability of Crystal forms of salts of Compounds of formula A
Taking the crystal form of the sodium salt of the compound shown in the formula A, the crystal form of the sulfate of the compound shown in the formula A and the crystal form of the maleate of the compound shown in the formula A to form a suspension in a solvent shown in the table 7, stirring the suspension for 3 days at room temperature, and carrying out crystal form stability investigation to compare with the result of the comparative example 1 in the patent CN 105315266A.
TABLE 7 statistics of the stability test results of the salt form and the free form of the compound of formula A in the solvent
From table 7, it can be seen that the final crystal forms of the compound shown in formula a in the free state in different solvents are inconsistent, which indicates that the compound shown in formula a in the free state is easy to produce mixed crystals in the process of drug formation, and the crystal form is difficult to control. The salt forms of the compound shown in the formula A have single crystal forms, so that the compound has more flexibility for solvent selection in the production process and more stable crystal forms.
All patent documents and non-patent publications cited in this specification are incorporated herein by reference in their entirety.
The above description is only an embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be made by those skilled in the art without inventive work within the technical scope of the present invention are included in the scope of the present invention.
Claims (27)
1. The sulfate of the compound shown in the formula A has a structure shown in the following formula:
2. sulphate according to claim 1, characterised in that it is crystalline.
3. Sulfate salt according to claim 1, characterized in that it is an anhydride, a hydrate or a non-solvate.
4. A crystalline form of the sulfate salt according to any one of claims 1 to 3, characterized in that the crystalline form has an X-ray powder diffraction pattern expressed in degrees 2 Θ using Cu-ka radiation with characteristic peaks at the following positions: 5.4 +/-0.2 degrees, 8.1 +/-0.2 degrees, 14.8 +/-0.2 degrees, 16.7 +/-0.2 degrees and 18.3 +/-0.2 degrees.
5. The crystalline form according to claim 4, characterized in that it has an X-ray powder diffraction pattern expressed in degrees 2 θ with characteristic peaks at the following positions: 5.4 +/-0.2 degrees, 8.1 +/-0.2 degrees, 14.8 +/-0.2 degrees, 15.6 +/-0.2 degrees, 16.7 +/-0.2 degrees, 18.3 +/-0.2 degrees, 21.0 +/-0.2 degrees, 22.0 +/-0.2 degrees, 22.9 +/-0.2 degrees, 25.2 +/-0.2 degrees and 26.3 +/-0.2 degrees.
6. A crystalline form according to claim 4, characterized in that it has an X-ray powder diffraction pattern expressed in degrees 2 θ with characteristic peaks and their relative intensities in the following positions:
7. the crystalline form of claim 4, wherein the crystalline form has an X-ray powder diffraction pattern as shown in figure 6.
8. The crystalline form according to any one of claims 4 to 7, characterized in that it has a Fourier infrared spectrum at wave number 1733cm -1 、1438cm -1 、1346cm -1 、1230cm -1 、1184 cm -1 、1109 cm -1 、1063 cm -1 、1009 cm -1 、885 cm -1 、854 cm -1 And 758cm -1 Has a characteristic peak.
9. A process for the preparation of the sulfate salt or crystalline form thereof of any one of claims 1 to 8, comprising the steps of:
in the process of selecting from C 1 ~C 4 Alcohol, C 3 ~C 4 In a solvent of ketone, cyclic ether, acetonitrile, water or a mixture thereof, mixing and reacting a compound represented by formula A and sulfuric acid, wherein the molar ratio of the compound represented by formula A to the sulfuric acid is 1:0.4 to 1, removing the solvent after the reaction is completed, and drying.
10. The method of claim 9, wherein the solvent is selected from methanol, ethanol, n-propanol, acetone, tetrahydrofuran, water, acetonitrile, or a mixture thereof.
11. The production method according to claim 9 or 10, wherein the molar ratio of the compound represented by the formula a to sulfuric acid is 1.
12. The method of claim 9 or 10, wherein the reaction is carried out at-10 to 60 ℃.
13. The method of claim 9 or 10, wherein the reaction is carried out at 10 to 40 ℃.
14. The method according to claim 9 or 10, wherein the reaction is carried out under stirring for 1 to 72 hours.
15. The method according to claim 9 or 10, wherein the reaction is carried out under stirring for 1 to 24 hours.
16. The method according to claim 9 or 10, wherein the drying temperature is 10 to 60 ℃.
17. The method according to claim 9 or 10, wherein the drying temperature is 10 to 40 ℃.
18. The method according to claim 9 or 10, wherein the drying time is 1 to 48 hours.
19. The method according to claim 9 or 10, wherein the drying time is 1 to 24 hours.
20. The preparation method according to claim 9 or 10, wherein the mass-to-volume ratio of the compound represented by formula A to the solvent is 1mg to 1mL.
21. The preparation method according to claim 9 or 10, wherein the mass volume ratio of the compound represented by the formula A to the solvent is 4mg to 1mL.
22. A pharmaceutical composition comprising a therapeutically and/or prophylactically effective amount of the sulfate salt of any one of claims 1-8, or a crystalline form thereof, and optionally at least one pharmaceutically acceptable carrier or excipient.
23. Use of the sulfate salt or the crystalline form thereof according to any one of claims 1 to 8 for the preparation of a medicament for the treatment and/or prevention of a disease or condition mediated by S1P1 receptors.
24. The use according to claim 23, wherein the disease or condition is selected from rheumatoid arthritis, multiple sclerosis, inflammatory bowel disease, autoimmune disease, chronic inflammatory disease, asthma, inflammatory neuropathy, arthritis, transplantation, crohn's disease, ulcerative colitis, lupus erythematosus, psoriasis, ischemia-reperfusion injury, solid tumors, diseases associated with angiogenesis, acute viral diseases, inflammatory bowel disease, insulin and non-insulin dependent diabetes mellitus and other related immune diseases.
25. The use according to claim 24, wherein the disease associated with angiogenesis is a vascular disease.
26. Use according to claim 23 or 24, wherein the disease or condition is selected from multiple sclerosis, rheumatoid arthritis, inflammatory bowel disease and psoriasis.
27. The use according to claim 23 or 24, wherein the disease or condition is crohn's disease or ulcerative colitis.
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| CN108299412B (en) * | 2017-01-11 | 2021-02-09 | 苏州康乃德生物医药有限公司 | Addition salt of S1P1 receptor agonist, crystal form and pharmaceutical composition thereof |
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