CN1127662C - Method for discriminating rice seed - Google Patents
Method for discriminating rice seed Download PDFInfo
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- CN1127662C CN1127662C CN00117133A CN00117133A CN1127662C CN 1127662 C CN1127662 C CN 1127662C CN 00117133 A CN00117133 A CN 00117133A CN 00117133 A CN00117133 A CN 00117133A CN 1127662 C CN1127662 C CN 1127662C
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- rice paddy
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Abstract
The present invention relates to a method for identifying rice seeds. The method comprises: whether rice seeds are genuine or fake is identified by comparing the curve of the superweak luminous intensity variation of the rice seeds to be tested after water absorption with the curve of the superweak luminous intensity variation of known rice seeds; whether an unknown rice sample is new or old is identified by measuring the average upstroke velocity value during the period of enhancing the superweak luminous intensity to the peak value of rice seeds after water absorption and the decay time constant value in the descending process. The method does not need to destroy a seed structure, and has the advantages of less use amount, easy realization and convenient operation.
Description
Technical field
The present invention relates to identify the new and old, true and pseudo-of rice paddy seed by the superweak luminescence intensity of measuring rice paddy seed.
Background technology
At present, judge the rice paddy seed vigor,, during the true and false, mainly ooze sproutung method as height, or utilization isozymogram analytic approach etc. by measuring germination percentage.It is that germinating capacity and its vigor of seed in hypertonic solution is remarkable positive correlation that height oozes the germination ratio juris, identifies rice paddy seed by detecting seed vitality in view of the above.
Judge the rice paddy seed vigor, during the true and false, available isozymogram analytic approach, its principle is that isodynamic enzyme is the product of gene expression, can directly reflect the hereditary variation in interbiotic hereditary difference and the phylogenetic process, isodynamic enzyme is charged big molecule, this charged particle can swimming in electric field, because the shape of isodynamic enzyme molecule, vary in size, amount of charge and character also have than big-difference, therefore its mobility speed is also inequality in certain kind and certain density gel, after electrophoresis finished, they, can show the different phenomenon of this mobility by dyeing to diverse location with regard to the difference swimming, the isodynamic enzyme band number of positions of arbitrary kind and shade etc. constitute " fingerprint " feature of this kind, therefore can utilize the isozyme electrophoresis analytical technology to carry out the authenticity of seed.
There is following shortcoming in said method: 1, the time long, for example ooze one week of needs that sproutung method is measured seed with high, the isozymogram analytic approach need reach one more than week sometimes; 2, destroy kernel texture, the seed large usage quantity can only disposablely be used, and carries out single index measurement, is unfavorable for the evaluation of rare seed.
Summary of the invention
The objective of the invention is to shortcoming at the prior art existence, a kind of authentication method of rice paddy seed is provided, identify the new and old, true and pseudo-of rice paddy seed by the superweak luminescence intensity of measuring rice paddy seed, can finish measurement at short notice, consumption is few, do not destroy kernel texture, reach simply, effect fast and accurately.
Biological superweak luminescence (wavelength is in the 200-800 nanometer) is the product in the biochemical metabolism process in the biosome.The detection of plant superweak luminescence is the new technology that twentieth century grew up after the eighties.
The inventor is through continuous exploration discovery, different rice paddy seed superweak luminescence intensity in imbibition phase (both incunabulums of seed germination) first few minutes have obvious specificity, utilize this superweak luminescence specificity can identify the new and old, true and pseudo-of rice paddy seed quickly, quantitatively.
The rice paddy seed of same kind, its superweak luminescence curve of suction back has consistance, the superweak luminescence curve and the known sample of rice paddy seed to be measured can be contrasted according to this specificity, thereby judges the true and pseudo-of unknown paddy rice sample.
The rice paddy seed of same kind, new seed is different with the superweak luminescence Strength Changes situation of year old seeds, promptly from its luminous enhancing in suction back until reaching the peak value average climbing speed value in during this period of time and the attenuation constant value difference the decline process constantly, can judge the new and old of unknown paddy rice sample according to this specificity; Rice paddy seed is newer, and vigor is high more, and it is big more that it adds behind the water increase of superweak luminescence intensity.
Average climbing speed: v=(p-s)/t, p is the peak value of superweak luminescence curve, and s is the average superweak luminous intensity of dry seeds, and t reaches the peak value required time for this kind after adding water.The inventor finds, under normal condition of storage (relative humidity 40～50%, 10～25 ℃ of temperature), the range of normal value of average climbing speed v that stores 3 months rice paddy seed superweak luminescence is 0.9～1.5 speed/second (being cps/s); The range of normal value of the peak value of superweak luminescence curve is 360～750cps.The range of normal value of average climbing speed v that stores 10 months rice paddy seed superweak luminescence is 0.40～0.50 speed/second (being cps/s); The range of normal value of the peak value of superweak luminescence curve is 200～300cps.The rice paddy seed superweak luminescence that stores 15 months does not have tangible peak value, and the superweak luminescence maximal value is 100～150cps.
Attenuation equation is Y=Ae
-t/ τ, A is the initial value of superweak decay of luminescence in the formula, and τ is a damping time constant, and t is the time.(relative humidity 40～50% under normal condition of storage, 10～25 ℃ of temperature), the scope that stores 3 months rice paddy seed τ value is 3000～3500, the rice paddy seed τ value scope that stores 10 months is 4800～5100, and the scope that stores 15 months rice paddy seed τ value is 10000～15000.
The authentication method of rice paddy seed of the present invention is with the superweak luminescence Strength Changes curve ratio of the superweak luminescence Strength Changes curve after the rice paddy seed to be measured suction and known water rice, identifies the true and puppet of rice paddy seed to be measured.Described rice paddy seed superweak luminescence Strength Changes curve known and to be measured must be measured under identical conditions (measuring temperature, humidity etc.).
Measure rice paddy seed to be measured suction back superweak luminescence strength-enhanced until reaching the peak value average climbing speed value in during this period of time and the attenuation constant value in the decline process constantly,, identify the new and old of paddy rice sample to be measured with the given value contrast.Described rice paddy seed superweak luminescence intensity known and to be measured must be measured under identical conditions (measuring temperature, humidity etc.).
The inventor is through exploration discovery, and it is the most obvious to measure its specificity of superweak luminescence Strength Changes curve that obtained in 40～60 minutes, be easier to identify rice paddy seed in view of the above and the true and false.
The superweak luminescence Strength Changes of measuring rice paddy seed can adopt the following step:
---the dry seeds of choosing is put into light transmission container, place camera bellows, the superweak luminescence intensity with single photon counting measurement mechanism measurement dry seeds reaches stable until it;
---add water to submerge seed, measure its superweak luminescence intensity, reach stable until it;
The time of above-mentioned measurement superweak luminescence intensity generally only needs about 1 hour.
Described dry seeds is the seed to be measured under the normal storage condition, i.e. atmospheric environment or room temperature condition.
The single photon counting measurement mechanism can be with commercially available instrument or the homemade device that is used to measure superweak luminescence intensity, for example the single photon counter, the U.S. " EG﹠amp that make of Japan " HAMAMATSU " company; G " single photon counter made of company.
The inventor finds that through investigative test when measuring down for 18～30 ℃, the result is more accurate.
The present invention compared with prior art has following advantage:
1, the present invention has utilized the obvious specificity of rice paddy seed superweak luminescence in the imbibition phase first few minutes, and is easy, measure the rice paddy seed vigor fast, quantitatively, judges its new and old, true and puppet.
2, the measurement mechanism that the present invention utilized is simple, invests lessly, realizes easily, and is easy and simple to handle.
3, seed consumption of the present invention is few, need not destroy kernel texture, and seed can re-use.
Description of drawings
Fig. 1 is that 3 months rice paddy seed of storage is adding water front and back superweak luminescence Strength Changes curve map;
Fig. 2 is that 10 months rice paddy seed of storage is adding water front and back superweak luminescence Strength Changes curve map;
Fig. 3 is that 15 months rice paddy seed of storage is adding water front and back superweak luminescence Strength Changes curve map.
The invention will be further described below by embodiment.
Embodiment
Embodiment 1
Identify the kind and the storage time (promptly new and old) of No. 1, No. 2, No. 3 rice paddy seed respectively
---choose size unanimity, the intact solid carbon dioxide rice of kind skin that will detect, memory period relative humidity 45% before measuring;
---during measurement, seed is put into 5 milliliters of quartz cuvette, place camera bellows, the single photon counter made from Japan " HAMAMATSU " company is measured the superweak luminescence 6 minutes of its dry seeds; Add 1 milliliter in water then, seed is not all had by water logging, continue to measure its superweak luminescence intensity 50 minutes, its superweak luminescence intensity is measured 25 ± 2 ℃ of temperature, relative humidity 45 ± 5% near stable; Obtain superweak luminescence Strength Changes curve 1 shown in Figure 1 (corresponding No. 1 seed), 2 (corresponding No. 2 seeds), 3 (corresponding No. 3 seeds).
With the curve of Fig. 1 and known curve ratio, identify the southern glutinous seed of curve 1 representative, excellent 998 seeds of curve 2 representatives, on behalf of Xian spy, curve 3 account for seed.
UBE (superweak luminescence intensity) mean value is 94.6 ± 7.6cps during south glutinous seed drying, add water after UBE rise rapidly, reach peak-peak 644.8 ± 28.9cps after 450 seconds, be 682% of dry seed, its average climbing speed is 1.22cps/s; After begin the decay, its attenuation equation is y=729.2e
-0.0003tIdentifying this seed storage time thus is 3 months.
UBE mean value is 95.4 ± 9.7cps during excellent 998 seed dryings, add water after UBE rise rapidly, reach peak-peak 470.6 ± 16.5cps after 330 seconds, be 493% of dry seed, its average climbing speed is 1.20cps/s; After begin the decay, its attenuation equation is y=551.6e
-0.0003tIdentifying this seed storage time thus is 3 months.
UBE mean value was 70.4 ± 7.7cps when Xian spy accounted for seed drying, add water after UBE rise rapidly, reach peak-peak 363.5 ± 13.2cps after 315 seconds, be 516% of dry seed, its average climbing speed is 0.93cps/s; After begin the decay, its attenuation equation is y=435.8e
-0.0003tIdentifying this seed storage time thus is 3 months.
Embodiment 2
Identify the kind and the storage time (promptly new) of No. 4, No. 5 rice paddy seeds respectively with old---choose big or small consistent, the intact solid carbon dioxide rice of kind skin that will detect, memory period relative humidity 50% before measuring;---during measurement, seed is put into 5 milliliters of quartz cuvette, place camera bellows, with the U.S. " EG﹠amp; G " single photon counter made of company measures, and surveys the superweak luminescence 5 minutes of its dry seeds; Add 2 milliliters in water then, seed is not all had by water logging, continue to measure its superweak luminescence intensity 50 minutes, near stable, measure 30 ± 2 ℃ of temperature, relative humidity 50 ± 5% until its superweak luminescence intensity; Obtain superweak luminescence Strength Changes curve 4 shown in Figure 2 (corresponding No. 4 seeds), 5 (corresponding fifth-seededs).
As shown in Figure 2, the UBE value of fifth-seeded before adding water is 58.7 ± 8.3cps, adds that UBE rises behind the water, reaching peak-peak 210.0 ± 14.5cps after 300 seconds, is 358% of dry seed, and its average climbing speed is 0.50cps/s, after begin the decay, its attenuation equation is y=244.62e
-0.0002t, identifying this seed storage time thus is 10 months.
The UBE value of No. 4 seeds before adding water is 63.1 ± 9.5cps, adds that UBE rises behind the water, reaches peak-peak 262 ± 12.5cps after 495 seconds, is 415% of dry seed, and its average climbing speed is 0.40cps/s, after begin decay, its attenuation equation is y=294e
-0.0002t, identifying this seed storage time thus is 10 months.
Embodiment 3
Identify the kind and the storage time (promptly new and old) of No. 6, No. 7 rice paddy seeds respectively
---choose size unanimity, the intact solid carbon dioxide rice of kind skin that will detect, memory period relative humidity 60% before measuring;
---during measurement, seed is put into 10 milliliters of clear glass cups, place camera bellows, with the U.S. " EG﹠amp; G " single photon counter made of company measures, and surveys the superweak luminescence 5 minutes of its dry seeds; Add 3 milliliters in water then, seed is not all had by water logging, continue to measure its superweak luminescence intensity 40 minutes, near stable, measure 25 ± 2 ℃ of temperature, relative humidity 60 ± 5% until its superweak luminescence intensity; Obtain superweak luminescence Strength Changes curve 6 shown in Figure 3 (corresponding No. 6 seeds), 7 (corresponding seventh-seededs).
As shown in Figure 3, the UBE value of No. 6 seeds before adding water is 52.2 ± 8.6cps, add water after UBE rise to some extent, do not have tangible peak value, maximal value is 141.8 ± 6.8cps, its attenuation equation is y=143.3e
-0.00008t, identifying this seed storage time thus is 15 months.
The UBE value of seventh-seeded before adding water is 49.1 ± 5.6cps, add water after UBE rise to some extent, do not have tangible peak value, maximal value is 120.5 ± 9.7cps, its attenuation equation is y=120.0e
-0.00009t, identifying this seed storage time thus is 15 months.
Claims (10)
1, a kind of true and pseudo-authentication method of rice paddy seed is characterized in that with the superweak luminescence Strength Changes curve ratio of the superweak luminescence Strength Changes curve after the rice paddy seed suction to be measured and known water rice identifying the true and puppet of rice paddy seed to be measured; Described rice paddy seed superweak luminescence Strength Changes curve known and to be measured must be measured under identical conditions.
2,, it is characterized in that the superweak luminescence Strength Changes of measuring rice paddy seed adopts the following step according to the true and pseudo-authentication method of the described rice paddy seed of claim 1:
---the dry seeds of choosing is put into light transmission container, place camera bellows, the superweak luminescence intensity with single photon counting measurement mechanism measurement dry seeds reaches stable until it;
---add water to submerge seed, measure its superweak luminescence intensity, reach stable until it.
3,, it is characterized in that described superweak luminescence Strength Changes curve is to measure the curve that obtained in 40～60 minutes according to the true and pseudo-authentication method of claim 1 or 2 described rice paddy seeds.
4, according to the true and pseudo-authentication method of claim 1 or 2 described rice paddy seeds, it is characterized in that measuring temperature is 18～30 ℃.
5, according to the true and pseudo-authentication method of claim 1 or 2 described rice paddy seeds, it is characterized in that described superweak luminescence Strength Changes curve is to measure the curve that obtained in 40～60 minutes, measuring temperature is 18～30 ℃.
6, a kind of new and old authentication method of rice paddy seed, it is characterized in that measuring rice paddy seed suction back superweak luminescence strength-enhanced to be measured until reaching the peak value average climbing speed value in during this period of time and the attenuation constant value in the decline process constantly, with the given value contrast, identify the new and old of paddy rice sample to be measured; Wherein, average climbing speed (p-s)/t, p is the peak value of superweak luminescence curve, and s is the average superweak luminous intensity of dry seeds, and t reaches the peak value required time for this kind after adding water; Described attenuation constant value is by attenuation equation Y=Ae
-t/ τObtain, A is the initial value of superweak decay of luminescence in the formula, and τ is a damping time constant, and t is the time; Described rice paddy seed superweak luminescence intensity known and to be measured must be measured under identical conditions.
7,, it is characterized in that the superweak luminescence Strength Changes of measuring rice paddy seed adopts the following step according to the new and old authentication method of the described rice paddy seed of claim 6:
---the dry seeds of choosing is put into light transmission container, place camera bellows, the superweak luminescence intensity with single photon counting measurement mechanism measurement dry seeds reaches stable until it;
---add water to submerge seed, measure its superweak luminescence intensity, reach stable until it.
8,, it is characterized in that described superweak luminescence Strength Changes curve is to measure the curve that obtained in 40～60 minutes according to the new and old authentication method of claim 6 or 7 described rice paddy seeds.
9, according to the new and old authentication method of claim 6 or 7 described rice paddy seeds, it is characterized in that measuring temperature is 18～30 ℃.
10, according to the new and old authentication method of claim 6 or 7 described rice paddy seeds, it is characterized in that described superweak luminescence Strength Changes curve is to measure the curve that obtained in 40～60 minutes, measuring temperature is 18～30 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00117133A CN1127662C (en) | 2000-06-06 | 2000-06-06 | Method for discriminating rice seed |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00117133A CN1127662C (en) | 2000-06-06 | 2000-06-06 | Method for discriminating rice seed |
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| CN1127662C true CN1127662C (en) | 2003-11-12 |
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| CN00117133A Expired - Fee Related CN1127662C (en) | 2000-06-06 | 2000-06-06 | Method for discriminating rice seed |
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| CN110118731A (en) * | 2019-05-27 | 2019-08-13 | 安徽省农业科学院烟草研究所 | Utilize the method for the full silty of the lossless Rapid identification of optics illumination photometer, waxy corn germplasm |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3339750A1 (en) * | 1983-11-03 | 1985-05-23 | Battelle-Institut E.V., 6000 Frankfurt | Process and apparatus for detecting radioactively irradiated organic materials |
| CN1053988A (en) * | 1991-01-04 | 1991-08-28 | 孙建华 | A kind of authentication method of distinguishing clover and daghestan sweet clover seed |
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2000
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3339750A1 (en) * | 1983-11-03 | 1985-05-23 | Battelle-Institut E.V., 6000 Frankfurt | Process and apparatus for detecting radioactively irradiated organic materials |
| CN1053988A (en) * | 1991-01-04 | 1991-08-28 | 孙建华 | A kind of authentication method of distinguishing clover and daghestan sweet clover seed |
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