CN112759554A - Compounds for the treatment of neurodegenerative diseases - Google Patents

Abstract

The invention relates to a compound with a substituted bicyclic structure, a pharmaceutically acceptable salt, a stereoisomer, a solvate, a polymorphism, a tautomer, an isotope compound, a metabolite or a prodrug thereof, and application thereof in preparing a medicament for preventing or treating polyglutamine (polyQ) related diseases.

Description

Compounds for the treatment of neurodegenerative diseases

Technical Field

The present invention relates to the field of biomedicine, and in particular to a compound having a substituted bicyclic structure, a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite or prodrug thereof, and use thereof for the preparation of a medicament for the prevention or treatment of polyglutamine (polyQ) -related diseases.

Background

Neurodegenerative diseases (Neurodegenerative disorders) refer to diseases in which abnormal death of central neurons leads to dysfunction of the nervous system. There is a lack of fundamental treatments for neurodegenerative diseases that can slow the progression of the disease. Many neurodegenerative diseases are caused by proteins of unknown activity. Methods that may be used to control protein levels, such as biological tools like RNAi or CRIPSR, are currently difficult to deliver, especially to the nervous system.

One possible therapeutic strategy is to control the levels of proteins affecting the disease by low molecular weight compounds (abbreviated compounds). Enhancing ubiquitination of disease proteins and targeting them to the proteasome degradation pathway by proteolytic targeting chimera (PROTAC) technology is an emerging approach, but only this approach relies on certain E3 ligases, which may not be present in disease cells. Also, proteasomes have limited proteolytic capacity and are less efficient at degrading certain large disease proteins or aggregates that lead to neurodegenerative diseases. Autophagy is an important protein degradation pathway, is generally present in eukaryotic cells, and has strong protein degradation capacity but low selectivity. Some studies increase protein degradation by enhancing autophagy, but such methods lack specificity.

The polyQ-related neurodegenerative disease is a neurodegenerative disease caused by variant proteins, and can be effectively treated by reducing the level of the variant proteins. In the case of Huntington's Disease (HD), which is the most common disease, which is a monogenic genetic disease, mutation of the CAG repeat region of exon1 of exon HTT of gene HTT contained in chromosome four of a patient results in amplification of the glutamine repeat region (polyQ) of the synthetic variant protein (mHTT). mHTT is susceptible to shearing, aggregation and toxicity, ultimately leading to specific neuronal dysfunction and death. Current methods of controlling mHTT levels by low molecular weight compounds lack specificity, may cause side effects, and are non-allelochemical, and the inability to distinguish mHTT from wild-type HTT proteins (wtHTT) results in reduced levels of wtHTT with important biological functions.

Similarly, taking spinocerebellar ataxia type 3 (SCA 3; also known as Machado-Joseph disease, MJD) as an example, it is the most common autosomal dominant spinocerebellar ataxia worldwide and a common polyQ-related disease second only to HD, caused by the fact that the increased number of CAG repeats in the Ataxin-3 gene (ATXN 3; also known as MJD1 gene) leads to the abnormal amplification of the C-terminal of the encoded protein ATXN 3. Several studies have shown that a reduced level of variant ATXN3 protein can bring about a therapeutic effect by acting on ATXN3 by means of siRNA, antisense oligonucleotides, etc. (Wang, Neuroscience,371,2018, 138-154). Some studies have focused on controlling the levels of variant ATXN3 protein by low molecular weight compounds. Studies of enhancing autophagy by compounds such as: menzies et al, Brain 2010,133: 93-104; studies to reduce the level of variant ATXN3 protein by modulating other targets are for example: costa MD, Brain,2016,139(11): 2891-. However, none of these studies has solved the problem of specificity well.

Accordingly, there is a need in the art for methods of targeting and/or reducing polyQ variant protein levels, thereby treating polyQ-related diseases, and for novel compounds that can be used to reduce polyQ variant protein levels.

Disclosure of Invention

In one aspect, the present invention provides a use of a compound of formula (I), or a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite or prodrug thereof, for the manufacture of a medicament for the prevention or treatment of a PolyQ-related neurodegenerative disease

Wherein:

ring A is a benzene ring;

ring B is a saturated or unsaturated five-or six-membered heterocyclic ring, phenyl ring, or six-membered heteroaromatic ring containing 1,2, or 3 heteroatoms each independently selected from N, O and S;

c ring is selected from C_6-10Aryl and five-to ten-membered heteroaryl, optionally substituted with one or more substituents each independently selected from R^X1Substituted with a group of (1);

L¹is a bond, or is C₁-C₆A hydrocarbon chain;

or, C ring is absent, and L¹Is absent;

R¹is ═ Y, where Y is O OR S, OR is OR⁷；

R²At each occurrence is independently selected from H, halogen, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, C_3-10Cycloalkyl radical, C_3-10Cycloalkyl radical-C_1-4Alkyl, three-to ten-membered heterocyclyl-C_1-4Alkyl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, ═ O, ═ S, ═ NR^a1、-OR^a1、-SR^a1、-NR^a1R^b1、-C(＝O)OR^a1、-C(＝O)NR^a1R^b1、-C(＝O)R^a1、-S(＝O)₂OR^a1、-S(＝O)₂R^a1、-S(＝O)₂NR^a1R^b1、-S(＝O)R^a1、-C(＝S)OR^a1、-C(＝S)NR^a1R^b1、-C(＝S)R^a1、-P(＝O)(OR^a1)OR^b1、-C(＝NR^a1)NR^b1R^c1-OCN, -SCN, -N ═ C ═ O, -NCS, where the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl or heteroaryl are optionally substituted with one or more substituents selected from halo, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, ═ O, ═ S, -OR^a2、-SR^a2、-NR^a2R^b2、-C(＝O)OR^a2、-C(＝O)NR^a2R^b2、-C(＝O)R^a2、-S(＝O)₂OR^a2、-S(＝O)₂R^a2、-S(＝O)₂NR^a2R^b2、-S(＝O)R^a2and-C (═ NR)^a2)NR^b2R^c2Substituted with the substituent(s);

or, two adjacent R²Together with the C atom to which they are attached form a five-to seven-membered lactam ring, optionally substituted by one or more groups selected from halogen, C_1-6Alkyl substituent substitution;

R³、R⁴、R⁵、R⁶each independently selected from H and R^X2；

Or, R⁴And R⁵Together with the C atom to which they are attached form a five-to seven-membered heterocyclic ring containing 1 or 2 heteroatoms each independently selected from N, O and S, and optionally substituted with one or more substituents selected from halogen and C_1-6Alkyl substituent substitution;

R^X1and R^X2Each occurrence independently selected from halogen, -NO₂、-CN、C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, -OR⁷、-SR⁷、-NR⁷R⁸、-C(＝O)OR⁷、-C(＝O)NR⁷R⁸、-OC(＝O)R⁷、-NC(＝O)R⁷R⁸、-C(＝O)R⁷、-S(＝O)₂OR⁷、-S(＝O)₂R⁷、-S(＝O)₂NR⁷R⁸、-OS(＝O)₂R⁷、-NS(＝O)₂R⁷R⁸、-S(＝O)R⁷Wherein said alkyl, alkenyl or alkynyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -SH, -S (C)_1-6Alkyl) and-S(C_3-6Cycloalkyl), -S (C)_1-4alkylene-C_3-6Cycloalkyl), -S (three-to seven-membered heterocyclyl), -S (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂O, -COOH and C_1-6Alkyl substituent substitution;

R⁷、R⁸each occurrence is independently selected from H, C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl radical, C_6-10aryl-C_1-4Alkyl, wherein the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl or aryl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-COOH、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl) substituted with a substituent;

R^a1、R^b1、R^c1、R^a2、R^b2、R^c2each occurrence is independently selected from H, C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl radical, C_6-10Aryl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, -OR^Y1、-SR^Y1、-NR^Y1R^Y2、-C(＝O)OR^Y1、-C(＝O)NR^Y1R^Y2、-C(＝O)R^Y1、-S(＝O)₂OR^Y1、-S(＝O)₂R^Y1、-S(＝O)₂NR^Y1R^Y2、-S(＝O)R^Y1Wherein said alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl OR heteroaryl is optionally substituted with one OR more substituents selected from halogen, ═ O, ═ S, -OR^Y3、-SR^Y3、-NR^Y3R^Y4、-C(＝O)R^Y3、-C(＝O)OR^Y3and-C (═ O) NR^Y3R^Y4Substituted with the substituent(s);

R^Y1、R^Y2、R^Y3、R^Y4each occurrence is independently selected from H, C_1-8Alkyl radical, C_3-10Cycloalkyl radical, C_3-10Cycloalkyl radical-C_1-4Alkyl, three-to ten-membered heterocyclyl-C_1-4Alkyl radical, C_6-10Aryl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, wherein the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, -OH, -SH, -NH₂Substituted with a substituent of ═ O and-COOH;

n is 1 or 2;

with the proviso that the compound of formula (I) does not include the following structure:

wherein R is³Is selected from-O (C)_1-6Alkyl), -O (C)_1-4alkylene-C_6-10Aryl groups); r¹⁰Is H;

and the compounds of formula (I) do not include the following structure:

wherein R is⁴is-O (C)_1-6Alkyl groups); r⁵Is halogen; r¹⁰Is CF₃；

And the compounds of formula (I) do not include the following structure:

wherein R is⁴Is selected from-O (C)_1-6Alkyl), -O (C)_1-4alkylene-C_6-10Aryl groups);

ring C being C_6-10Aryl, optionally substituted with one or more substituents each independently selected from R^X1Substituted with a group of (1);

and the compounds of formula (I) do not include the following structure:

wherein R is⁴Selected from-OH, -O (C)_1-6Alkyl groups); r⁵Is halogen; the C ring is a five-to ten-membered heteroaryl group optionally substituted with one or more substituents each independently selected from R^X1Is substituted with a group (b).

In one embodiment, the neurodegenerative disease is spinocerebellar ataxia (e.g., type 1,2, 3, 6, 7, 12, 17), dentate nucleus-substantia nigra-red nucleus-globus-thalamic atrophy, huntington's disease, huntington-like syndrome-2, or spinobulbar muscular atrophy, particularly huntington's disease.

Drawings

FIG. 1 Effect of compounds on protein levels containing repeated regions of glutamine in HEK293T cells.

FIG. 2 affinity binding curves of OI-RD assayed compounds with different concentrations of full-length HTT. The vertical dashed lines in the figure indicate the onset of the association and dissociation phases of the affinity binding. The dashed curve is the result of a global fit to the Langmuir reaction model.

FIG. 3 affinity binding curves of MST-assayed compounds to full-length HTT.

FIG. 4 Compound Pair Hdh^Q140/Q7Effect of cortical neuronal HTT levels in mice.

FIG. 5. Compound 1 vs. Hdh^Q7/Q7Effect of cortical neuronal HTT levels in mice.

FIG. 6 detection of N-terminal fragments of mHTT with antibodies MW1 and 3B5H 10.

FIG. 7 Hdh after compound treatment^Q140/Q7And (5) detecting the cell activity of mouse cortical neurons.

FIG. 8. Effect of compounds on mHTT levels in HD patients at a concentration of 100 nM.

Figure 9. effect of compounds on immortalized fibroblast mHTT levels in HD patients.

Figure 10 compound 2 reduces mHTT levels in fibroblasts of immortalized HD patients.

Figure 11 effect of compounds on mHTT levels in neurons inducing stem cell (iPSC) differentiation in HD patients.

Figure 12 effect of compounds on neuronal apoptosis inducing stem cell (iPSC) differentiation in HD patients. The ordinate spans 50 μ M.

Figure 13 effect of compounds on neuronal apoptosis inducing stem cell (iPSC) differentiation in HD patients.

FIG. 14. Effect of compounds on the level of Drosophila Huntington's disease mHTT.

Figure 15 effect of compounds on survival of drosophila huntington's disease.

Figure 16 effect of compounds on the ability of huntington's disease drosophila to crawl.

FIG. 17. Effect of intracerebroventricular injection of compounds on the levels of cortical mHTT and wtHTT in Huntington's disease mice.

FIG. 18 Effect of intraperitoneal injection of Compound 2 on cortical mHTT and wtHTT levels in Huntington's disease mice.

FIG. 19 Effect of intraperitoneal injection of Compound 2 on the level of striatal mHTT and wtHTT in Huntington's disease mice.

FIG. 20 detection of mHTT aggregates in the cortex of Huntington's disease mice following intraperitoneal injection of Compound 2.

Figure 21. effect of intraperitoneal injection of compound 2 on huntington's disease mouse behavioral deficits.

FIG. 22. Effect of compounds on the level of ATXN3 protein in fibroblasts from spinocerebellar ataxia type 3 patients.

Detailed Description

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present application will control. When a trade name appears herein, it is intended to refer to its corresponding commodity or its active ingredient. All patents, published patent applications and publications cited herein are hereby incorporated by reference.

General terms and definitions

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present application will control. When a trade name appears herein, it is intended to refer to its corresponding commodity or its active ingredient. All patents, published patent applications and publications cited herein are hereby incorporated by reference.

The terms "comprising," "including," "having," "containing," or "involving," and other variations thereof herein, are inclusive or open-ended and do not exclude additional unrecited elements or method steps. It will be understood by those skilled in the art that terms such as "including" and "comprising" encompass the meaning of "consisting of ….

The term "one or more" or similar expressions "at least one" may mean, for example, 1,2, 3,4, 5, 6, 7, 8, 9, 10 or more(s).

When the lower and upper limits of a range of values are disclosed, any value falling within the range and any included range is specifically disclosed. In particular, each range of values disclosed herein is to be understood as meaning each and every value and range encompassed within the broader range. For example, the expression "ATXN 1 with a polyQ length ≧ 40" may cover the case where the polyQ length ≧ 41. For example, "ATXN 2 with a polyQ length ≧ 33" can cover the case where the polyQ length ≧ 34. For example, "ATXN 3 for a polyQ length ≧ 41" may cover the case where the polyQ length ≧ 62, and may cover the case where the polyQ length is 74, for example. For example, "ATXN 3 for a polyQ length < 41" may cover the case where the polyQ length is 27. For example, "ATXN 7 for a polyQ length ≧ 19" can encompass the case of a polyQ length ≧ 38. For example, a "TBP of polyQ length ≧ 44" may cover the case where the polyQ length ≧ 45. For example, "ATN 1 with a polyQ length ≧ 39" can cover the case where the polyQ length ≧ 49. For example, an "HTT with a polyQ length ≧ 36" can encompass cases where the polyQ length is 47, 49, 55, 68, 72, 73, 111, 128, or 140, and so forth. For another example, an "HTT of polyQ length < 36" may cover a case where the polyQ length is 7, 16, 19, 23, or 25, etc. For example, "AR with a polyQ length ≧ 37" may encompass the case with a polyQ length ≧ 38.

The expression m-n as used herein refers to the range of m to n as well as to the subranges comprised of individual point values therein as well as to individual point values. For example, the expression "C₁-C₈"or" C_1-8"covers the range of 1 to 8 carbon atoms and is to be understood to also cover any subrange therein as well as each point value, e.g. C₂-C₅、C₃-C₄、C₁-C₂、C₁-C₃、C₁-C₄、C₁-C₅、C₁-C₆、C₁-C₇Etc. and C₁、C₂、C₃、C₄、C₅、C₆、C₇、C₈And the like. For example, the expression "C₃-C₁₀"or" C_3-10"should also be understood in a similar manner, e.g. to cover any sub-ranges and point values comprised therein, e.g. C₃-C₉、C₆-C₉、C₆-C₈、C₆-C₇、C₇-C₁₀、C₇-C₉、C₇-C₈、C₈-C₉Etc. and C₃、C₄、C₅、C₆、C₇、C₈、C₉、C₁₀And the like. Also for example, the expression "three to ten elements" should be understood to encompass any subrange therein as well as each point value, such as three to five elements, three to six elements, three to seven elements, three to eight elements, four to five elements, four to six elements, four to seven elements, four to eight elements, five to seven elements, five to eight elements, six to eight elements, nine to ten elements, etc., as well as three, four, five, six, seven, eight, nine, ten elements, etc. Other similar expressions in this document should be understood in a similar manner.

The terms "optionally" or "optionally" mean that the subsequently described event or circumstance may or may not occur, and that the description includes instances where said event or circumstance occurs and instances where it does not.

The terms "substituted" and "substituted" mean that one or more (e.g., one, two, three, or four) hydrogens on the designated atom is replaced with a selection from the indicated group, provided that the designated atom's normal valency in the current situation is not exceeded and that the substitution results in a stable compound. Combinations of substituents and/or variables are permissible only if such combinations result in stable compounds. When it is stated that a substituent is absent, it is understood that the substituent may be one or more hydrogen atoms, provided that the structure is such that the compound attains a stable state.

If a substituent is described as "optionally … substituted," the substituent may be unsubstituted or may be substituted. If an atom or group is described as optionally substituted with one or more of a list of substituents, one or more hydrogens on the atom or group may be replaced with an independently selected, optional substituent. When the substituent is oxo (i.e., ═ O), it means that two hydrogen atoms are replaced.

Unless indicated, as used herein, the point of attachment of a substituent may be from any suitable position of the substituent. When a bond of a substituent is shown through a bond connecting two atoms in a ring, then such substituent may be bonded to any ring atom in the substitutable ring.

When any variable (e.g., R), and the variable bearing the label (e.g., R)^X1、R^X2、R⁷、R⁸、R^a1、R^b1、R^c1、R^a2、R^b2、R^c2Etc.) in the composition or structure of a compound, which at each occurrence is defined independently in each instance. For example, if a group is substituted with 0,1, 2,3, or 4R substituents, the group can optionally be substituted with up to four R substituents, and the options for each R substituent in each case are independent of each other.

The term "halogen" or "halo" is understood to mean a fluorine (F), chlorine (Cl), bromine (Br) or iodine (I) atom, preferably a fluorine, chlorine, bromine atom.

The term "alkyl" refers to a straight or branched chain saturated aliphatic hydrocarbon group consisting of carbon and hydrogen atoms, which is attached to the rest of the molecule by a single bond. The "alkyl" group may have 1 to 8 carbon atoms, i.e. "C_1-8Alkyl radicals ", e.g. C_1-4Alkyl radical, C_1-3Alkyl radical, C_1-2Alkyl radical, C₃Alkyl radical, C₄Alkyl radical, C_1-6Alkyl radical, C_3-6An alkyl group. Non-limiting examples of alkyl groups include, but are not limited to, methyl, ethyl, propyl, butyl, pentyl, hexyl, isopropyl, isobutyl, sec-butyl, tert-butyl, isopentyl, 2-methylbutyl, 1-ethylpropyl, 1, 2-dimethylpropyl, neopentyl, 1-dimethylpropyl, 4-methylpentyl, 3-methylpentyl, 2-methylpentyl, 1-methylpentyl, 2-ethylbutyl, 1-ethylbutyl, 3-dimethylbutyl, 2-dimethylbutyl, 1-dimethylbutyl, 2, 3-dimethylbutyl, 1, 3-dimethylbutyl, or 1, 2-dimethylbutyl, or isomers thereof.

The term "alkylene", as used herein alone or in combination with other groups, refers to a straight or branched chainSaturated divalent hydrocarbon group of (1). For example, the term "C_1-6Alkylene "means an alkylene group having 1 to 6 carbon atoms, such as methylene, ethylene, propylene, butylene, pentylene, hexylene, 1-methylethylene, 2-methylethylene, methylpropylene, ethylpropylene, or the like.

The term "alkenyl" refers to a straight or branched chain unsaturated aliphatic hydrocarbon group having at least one double bond, consisting of carbon atoms and hydrogen atoms. The alkenyl group may have 2 to 8 carbon atoms, i.e. "C_2-8Alkenyl radicals, e.g. C_2-4Alkenyl radical, C_3-4An alkenyl group. Non-limiting examples of alkenyl groups include, but are not limited to, vinyl, allyl, (E) -2-methylvinyl, (Z) -2-methylvinyl, (E) -but-2-enyl, (Z) -but-2-enyl, (E) -but-1-enyl, (Z) -but-1-enyl, and the like.

The term "alkynyl" refers to a straight or branched chain unsaturated aliphatic hydrocarbon group having at least one triple bond composed of carbon atoms and hydrogen atoms. The alkynyl group may have 2 to 8 carbon atoms, i.e. "C_2-8Alkynyl radicals, e.g. C_2-4Alkynyl, C_3-4Alkynyl. Non-limiting examples of alkynyl groups include, but are not limited to, ethynyl, prop-1-ynyl, prop-2-ynyl, but-1-ynyl, but-2-ynyl, but-3-ynyl, and the like.

The term "cycloalkyl" refers to a saturated or unsaturated, non-aromatic cyclic hydrocarbon group consisting of carbon and hydrogen atoms, preferably containing 1 or 2 rings. The cycloalkyl group may be a monocyclic, fused polycyclic, bridged or spiro ring structure. The cycloalkyl group may have 3 to 10 carbon atoms, i.e. "C_3-10Cycloalkyl radicals ", e.g. C_3-8Cycloalkyl radical, C₅Cycloalkyl radical, C₆Cycloalkyl radical, C₇A cyclic hydrocarbon group. Non-limiting examples of cycloalkyl groups include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, bicyclo [2.2.1 ] n]Heptyl and spiro [3.3 ]]Heptyl, and the like. The term also encompasses the case where the C atom may be substituted by oxo (═ O).

The term "heterocyclyl" or "heterocycloalkyl" refers to a monocyclic or bicyclic ring system (three to ten, three to eight, three to seven, four to six) having, for example, 3 to 10 (suitably 3 to 8, more suitably 3 to 7, especially 4 to 6) ring atoms, wherein at least one ring atom (e.g. 1,2 or 3) is a heteroatom selected from N, O and S, and the remaining ring atoms are C. The ring system may be saturated (also understood as corresponding "heterocycloalkyl") or unsaturated (i.e., having one or more double and/or triple bonds within the ring). "Heterocyclyl" or "heterocycloalkyl" have no aromaticity. The term also covers the case where the C atom may be substituted by oxo (═ O) and/or the S atom on the ring may be substituted by 1 or 2 oxo (═ O).

Heterocyclyl groups may be, for example, four-membered rings, such as azetidinyl, oxetanyl; or five-membered rings, such as tetrahydrofuranyl, dioxanyl (dioxalinyl), pyrrolidinyl, imidazolidinyl, pyrazolidinyl, pyrrolinyl, oxopyrrolidinyl, 2-oxoimidazolidin-1-yl; or a six-membered ring, such as tetrahydropyranyl, piperidinyl, morpholinyl, dithianyl, thiomorpholinyl, piperazinyl, 1-dioxo-1, 2-thiazinan-2-yl, or trithianyl; or seven-membered rings, e.g. diaza

A base ring. Optionally, the heterocyclic group may be benzo-fused.

The heterocyclic group may be bicyclic, without limitation, for example a five-membered and five-membered ring such as a hexahydrocyclopenta [ c ] pyrrol-2 (1H) -yl) ring; or five-membered and six-membered bicyclic rings, such as the hexahydropyrrolo [1,2-a ] pyrazin-2 (1H) -yl ring.

As mentioned above, the heterocyclic ring may be unsaturated, i.e. it may contain one or more double bonds, without being limited thereto, e.g. the unsaturated heterocyclic ring containing a nitrogen atom may be 1, 6-dihydropyrimidine, 1, 2-dihydropyrimidine, 1, 4-dihydropyrimidine, 1, 6-dihydropyridine, 1, 2-dihydropyridine, 1, 4-dihydropyridine, 2, 3-dihydro-1H-pyrrole, 3, 4-dihydro-1H-pyrrole, 2, 5-dihydro-1H-pyrrolyl, 4H- [1,3,4] thiadiazinyl, 4, 5-dihydrooxazolyl or 4H- [1,4] thiazinyl ring, the unsaturated heterocyclic ring containing an oxygen atom may be 2H-pyran, 4H-pyran, 2, 3-dihydrofuran, the unsaturated heterocyclic ring containing a sulfur atom may be 2H-thiopyran or 4H-thiopyran. The heterocyclic ring may be benzo-fused, without limitation, such as a dihydroisoquinolinyl ring.

The term "aryl" refers to an aromatic cyclic group that is an all-carbon monocyclic or fused polycyclic (e.g., bicyclic) ring having a conjugated pi-electron system. For example, the aryl group may have 6 to 14 carbon atoms, suitably 6 to 10, more suitably 6 or 10. Examples of aryl groups include, but are not limited to, phenyl, naphthyl, and anthracenyl and the like.

The term "heteroaryl" is understood as preferably meaning a monovalent monocyclic, bicyclic or tricyclic aromatic ring system having 5, 6, 7, 8, 9 or 10 ring atoms ("five-to ten-membered heteroaryl"), in particular 5 or 6 or 9 or 10 ring atoms, and comprising at least one, suitably 1 to 4, more suitably 1,2 or 3, heteroatom(s), which may be the same or different, among the ring atoms, such as oxygen, nitrogen or sulfur. Furthermore, the heteroaryl groups can be benzo-fused in each case. In particular, heteroaryl is selected from thienyl, furyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, triazolyl, thiadiazolyl and the like, and their benzo derivatives, such as benzofuryl, benzothienyl, benzoxazolyl, benzisoxazolyl, benzimidazolyl, benzotriazolyl, indazolyl, indolyl, isoindolyl and the like; or pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, triazinyl, and the like, and benzo derivatives thereof, such as quinolyl, quinazolinyl, isoquinolyl, and the like; or azocinyl, indolizinyl, purinyl and the like, and benzo derivatives thereof; or cinnolinyl, phthalazinyl, quinazolinyl, quinoxalinyl, naphthyridinyl, carbazolyl, acridinyl and the like.

The term "C₁-C₆The hydrocarbon chain "means a chain-like group composed of carbon atoms and hydrogen atoms, which may be straight-chain or branched, and contains 1 to 8 (particularly 1 to 5, for example 1,2, 3,4 or 5) carbon atoms. The hydrocarbon chain may be saturated (i.e., C)₁-C₆Alkylene) or may be unsaturated, i.e. may contain one or more (preferably 1) carbon-carbon double or triple bonds.

The alkylene group may have 1 to 8Carbon atoms, i.e. "C_1-6Alkylene radicals, e.g. C_1-5Alkylene radical, C_1-4Alkylene radical, C₁-₃Alkylene radical, C_1-2Alkylene radical, C₃Alkylene, and C₁Alkylene, i.e. methylene. Non-limiting examples of alkylene groups include, but are not limited to, methylene (-CH)₂-), 1-ethylidene (-CH (CH)₃) -), 1, 2-ethylene (-CH)₂CH₂-), 1-propylene (-CH (CH)₂CH₃) -), 1, 2-propylene (-CH)₂CH(CH₃) -), 1, 3-propylene (-CH)₂CH₂CH₂-) 1, 4-butylene (-CH₂CH₂CH₂CH₂-) and the like.

The term "neurodegenerative disease" refers to a disease resulting from loss or pathology of neurons and/or their myelin sheaths. Characteristic pathological structures, such as insoluble aggregates of proteins, can be observed in brain neurons of patients with neurodegenerative diseases. Insoluble aggregates may cause cytotoxicity, which in turn leads to neuronal loss and disease development.

The term "polyQ" or "polyglutamine" refers to a glutamine repeat region in a protein. Glutamine is encoded by cytosine-adenine-guanine (CAG) in genes, and the length of the glutamine repeat region is related to the number of CAG repeats in the exon of the gene, so that an increased number of CAG repeats in the exon of the gene results in the amplification of the glutamine repeat region of the synthesized protein. polyQ aberrant amplification proteins are known to be associated with several neurodegenerative diseases. As used herein, the number of CAG repeats in an exon can be expressed in the gene name by the form of "Q + number", e.g., Q25 or Q72, representing 25 repeats or 72 repeats of CAG in an exon, respectively. The length of the glutamine repeat region can be indicated in the protein name by the form "Q + number" as above, e.g. Q27 or Q73, indicating a glutamine repeat region of 27Q (glutamines) or 73Q, respectively. Herein, the forms "Q + numbers" indicate that CAG repeats or glutamine repeats are both consecutive repeats. Unless otherwise indicated, polyQ length herein refers to the length of a continuously repeating glutamine region.

The term "polyQ-related neurodegenerative disease" refers to a neurodegenerative disease associated with abnormal amplification of polyQ, or that responds to protein levels containing amplified polyQ, is a group of neurodegenerative diseases that are clinically and genetically heterogeneous. By "normal polyQ" is meant that the protein in a normal physiological state has polyQ less than a specified number in length. Correspondingly, "polyQ-aberrant amplification" means that the polyQ length of the protein is greater than the normal length. For diseases or pathological conditions, polyQ will be longer. As an example, polyQ-related neurodegenerative diseases include, but are not limited to, spinocerebellar ataxia (SCA) type 1 (polyQ length ≧ 41), type 2 (polyQ length ≧ 34), type 3 (polyQ length ≧ 62), type 7 (polyQ length ≧ 38), type 12 (polyQ length ≧ 46), type 17 (polyQ length ≧ 45); and dentatorubral-pallidoluysian atrophy (DRPLA, polyQ length ≥ 49), Huntington's Disease (HD, polyQ length ≥ 36), and spinal-bulbar muscular atrophy (SBMA, polyQ length ≥ 38). These diseases are caused by the amplification of the CAG repeat region in ATXN1, ATXN2, ATXN3, ATXN7, ATXN12, TBP, ATN1, HTT and AR genes, respectively (Lesley Jones et al, DNA repair in the trinuclear repeat disorders, Lancet neurol.2017; 16: 88-96). Among them, spinocerebellar ataxia type 3 (SCA3, also known as Machado-Joseph disease, MJD) is the most common autosomal dominant spinocerebellar ataxia worldwide and the common polyQ-related disease second to HD, and is caused by the fact that the C-terminal of the encoded protein ATXN3 is abnormally amplified due to the increase of the number of CAG repeats of Ataxin-3 gene (ATXN 3; also known as MJD1 gene). Examples of normal polyQ proteins described herein include, but are not limited to, ATXN1 of polyQ length <40, ATXN2 of polyQ length <33, ATXN3 of polyQ length <41, ATXN7 of polyQ length <19, ATXN12 of polyQ length <46, TBP of polyQ length <44, ATN1 of polyQ length <39, HTT of polyQ length <36, and AR of polyQ length < 37. Correspondingly, examples of the polyQ aberrant amplification proteins described herein include, but are not limited to, ATXN1 for a polyQ length of ≧ 40, ATXN2 for a polyQ length of ≧ 33, ATXN3 for a polyQ length of ≧ 41, ATXN7 for a polyQ length of ≧ 19, ATXN12 for a polyQ length of ≧ 46, TBP for a polyQ length of ≧ 44, ATN1 for a polyQ length of ≧ 39, HTT for a polyQ length of ≧ 36, and AR for a polyQ length of ≧ 37.

The term "pharmaceutically acceptable" means having a reasonable benefit to risk ratio and being effective for the intended use without undue toxicity, irritation, allergic response, and the like, upon contact with the tissue of a patient within the scope of normal medical judgment.

Pharmaceutically acceptable salts of the compounds of the present invention include acid addition salts and base addition salts thereof. Suitable acid addition salts are formed from acids which form pharmaceutically acceptable salts. Examples include hydrochloride, acetate, aspartate, benzoate, bicarbonate/carbonate, glucoheptonate, gluconate, nitrate, orotate, palmitate and other similar salts. Suitable base addition salts are formed from bases which form pharmaceutically acceptable salts. Examples include aluminum salts, arginine salts, choline salts, magnesium salts, and other similar salts. Methods for preparing pharmaceutically acceptable salts of the compounds of the present invention are known to those skilled in the art.

The compounds of the present invention may exist in specific geometric or stereoisomeric forms. The present invention contemplates all such compounds, including cis and trans isomers, (-) -and (+) -enantiomers, (R) -and (S) -enantiomers, diastereomers, (D) -isomers, (L) -isomers, as well as racemic and other mixtures thereof, such as enantiomerically or diastereomerically enriched mixtures, all of which are within the scope of the present invention. Additional asymmetric carbon atoms may be present in substituents such as alkyl groups. All such isomers, as well as mixtures thereof, are included within the scope of the present invention. In certain embodiments, preferred compounds are those that exhibit more advantageous biological activity as isomers. Purified or partially purified isomers and stereoisomers, or racemic or diastereomeric mixtures of the compounds of the invention are also included within the scope of the invention. Purification and isolation of such materials can be accomplished by standard techniques known in the art.

Optically pure enantiomers can be obtained by resolution of the racemic mixture according to conventional methods, for example by formation of diastereomeric salts using optically active acids or bases, or by formation of covalent diastereomers. Mixtures of diastereomers may be separated into the individual diastereomers by methods known in the art (e.g., by chromatography or fractional crystallization) based on their physical and/or chemical differences. The optically active enantiomeric base or acid is then released from the separated diastereomeric salt. Another method of separating racemic enantiomers may use chiral chromatography (e.g., a chiral HPLC column), and the separated chiral isomers may be subjected to conventional derivatization prior to separation or may not be derivatized, depending on which method may achieve more efficient separation of chiral isomers. Enzymatic methods can also be used to separate derivatized or underivatized chiral isomers. Likewise, the optically pure compound of the present invention can be obtained by chiral synthesis using an optically active raw material.

In addition, the compounds of the present invention may exist in tautomeric forms. The invention includes all possible tautomers of the compounds of the invention, also including the forms of a single tautomer or any mixture of said tautomers in any ratio.

The compounds of the invention may be present in the form of solvates, preferably hydrates, wherein the compounds of the invention comprise as structural element of the crystal lattice of the compound a polar solvent, such as in particular water, methanol or ethanol. The amount of polar solvent, particularly water, may be present in stoichiometric or non-stoichiometric proportions.

The invention also encompasses all possible crystalline forms or polymorphs of the compounds of the invention, which may be single polymorphs or mixtures of more than one polymorph in any ratio.

The invention also includes all pharmaceutically acceptable isotopically-labeled compounds, which are identical to those of the present invention, except that one or more atoms are replaced by an atom having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number prevailing in nature.

Also included within the scope of the present invention are metabolites of the compounds of the present invention, i.e., substances formed in vivo upon administration of the compounds of the present invention. Metabolites of a compound can be identified by techniques well known in the art, and their activity can be characterized by assay methods. Such products may result, for example, from oxidation, reduction, hydrolysis, amidation, deamidation, esterification, enzymatic hydrolysis, etc. of the administered compound. Accordingly, the present invention includes metabolites of the compounds of the present invention, including compounds made by the process of contacting the compounds of the present invention with a mammal for a time sufficient to produce a metabolite thereof.

The present invention further includes within its scope prodrugs of the compounds of the present invention which are certain derivatives of the compounds of the present invention which may themselves have little or no pharmacological activity which, when administered into or onto the body, may be converted to the compounds of the present invention having the desired activity by, for example, hydrolytic cleavage. Typically such prodrugs will be functional derivatives of the compounds which are readily convertible in vivo into the desired therapeutically active compound. For a review of Prodrugs and methods of making them, see, e.g., J.Rautio et al, Nature Reviews Drug Discovery (2008)7, 255-. Prodrugs of the invention may be prepared, for example, by substituting certain moieties known to those skilled in the art as "pro-moieties" for appropriate functional groups present in the compounds of the invention.

The term "polymorph" or "polymorph" refers to a single polymorph or a mixture of more than one polymorph in any ratio.

The term "crystalline form" or "crystalline" refers to any solid substance that exhibits a three-dimensional ordering, as opposed to an amorphous solid substance, which produces a characteristic X-ray powder diffraction pattern having well-defined peaks.

The term "amorphous" refers to any solid substance that is not ordered in three dimensions.

The term "hydrate" describes a solvate comprising a drug with a stoichiometric or non-stoichiometric amount of water.

The term "pharmaceutically acceptable carrier" refers to those substances which do not significantly stimulate the organism and do not impair the biological activity and performance of the active compound. "pharmaceutically acceptable carriers" include, but are not limited to, glidants, sweeteners, diluents, preservatives, dyes/colorants, flavoring agents, surfactants, wetting agents, dispersants, disintegrants, stabilizers, solvents, or emulsifiers. Non-limiting examples of such carriers include calcium carbonate, calcium phosphate, various sugars and types of starch, cellulose derivatives, gelatin, vegetable oils, polyethylene glycols, and the like.

The terms "administration" or "administering" and the like refer to a method that can enable a compound or composition to be delivered to a desired site of biological action. These methods include, but are not limited to, oral or parenteral (including intracerebroventricular, intravenous, subcutaneous, intraperitoneal, intramuscular, intravascular injection or infusion), topical, rectal administration, and the like. Especially for injection or oral administration.

As used herein, the term "treating" includes alleviating, or ameliorating a disease or condition, preventing other conditions, ameliorating or preventing underlying metabolic factors of a condition, inhibiting a disease or condition, e.g., arresting the development of a disease or condition, alleviating a disease or condition, promoting remission of a disease or condition, or arresting signs of a disease or condition, and extends to include prevention. "treating" also includes achieving a therapeutic benefit and/or a prophylactic benefit. Therapeutic benefit refers to eradication or amelioration of the condition being treated. In addition, therapeutic benefit is achieved by eradicating or ameliorating one or more physiological signs associated with the underlying disease, and amelioration of the disease in the patient is observed, although the patient may still be suffering from the underlying disease. Prophylactic benefit refers to the use of a composition by a patient to prevent the risk of a disease, or the administration of a composition by a patient presenting with one or more physiological conditions of a disease, although the disease has not yet been diagnosed.

The terms "active ingredient," "therapeutic agent," "active substance," or "active agent" refer to a chemical entity that is effective in treating or preventing a target disorder, disease, or condition.

The terms "effective amount", "therapeutically effective amount" or "prophylactically effective amount" with respect to a drug, drug unit or active ingredient refers to a sufficient amount of the drug or pharmaceutical agent that is acceptable for the side effects but achieves the desired effect. The determination of an effective amount varies from person to person, depending on the age and general condition of the individual and also on the particular active substance, and an appropriate effective amount in an individual case can be determined by the person skilled in the art in the light of routine tests.

As used herein, "individual" includes a human or non-human animal. Exemplary human individuals include human individuals (referred to as patients) having a disease (e.g., a disease described herein) or normal individuals. "non-human animals" in the context of the present invention include all vertebrates, such as non-mammals (e.g., birds, amphibians, reptiles) and mammals, such as non-human primates, livestock and/or domesticated animals (e.g., sheep, dogs, cats, cows, pigs, etc.).

The following detailed description is intended to illustrate non-limiting embodiments and to enable others skilled in the art to more fully understand the teachings of the present invention, its principles, and its practical application, so that others skilled in the art may modify and implement the invention in various forms, which are best suited to the requirements of a particular use.

Compounds of the invention and uses thereof

In one aspect, the present invention provides a use of a compound of formula (I), or a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite or prodrug thereof, for the manufacture of a medicament for the prevention or treatment of a polyQ-related neurodegenerative disease

Wherein:

ring A is a benzene ring;

ring B is a saturated or unsaturated five-or six-membered heterocyclic ring, phenyl ring, or six-membered heteroaromatic ring containing 1,2, or 3 heteroatoms each independently selected from N, O and S;

c ring is selected from C_6-10Aryl and five-to ten-membered heteroaryl, optionally substituted with one or more substituents each independently selected from R^X1Substituted with a group of (1);

L¹is a bond, or is C₁-C₆A hydrocarbon chain;

or, C ring is absent, and L¹Is absent;

R¹is ═ Y, where Y is O OR S, OR is OR⁷；

R²At each occurrence is independently selected from H, halogen, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, C_3-10Cycloalkyl radical, C_3-10Cycloalkyl radical-C_1-4Alkyl, three-to ten-membered heterocyclyl-C_1-4Alkyl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, ═ O, ═ S, ═ NR^a1、-OR^a1、-SR^a1、-NR^a1R^b1、-C(＝O)OR^a1、-C(＝O)NR^a1R^b1、-C(＝O)R^a1、-S(＝O)₂OR^a1、-S(＝O)₂R^a1、-S(＝O)₂NR^a1R^b1、-S(＝O)R^a1、-C(＝S)OR^a1、-C(＝S)NR^a1R^b1、-C(＝S)R^a1、-P(＝O)(OR^a1)OR^b1、-C(＝NR^a1)NR^b1R^c1-OCN, -SCN, -N ═ C ═ O, -NCS, where the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl or heteroaryl are optionally substituted with one or more substituents selected from halo, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, ═ O, ═ S, -OR^a2、-SR^a2、-NR^a2R^b2、-C(＝O)OR^a2、-C(＝O)NR^a2R^b2、-C(＝O)R^a2、-S(＝O)₂OR^a2、-S(＝O)₂R^a2、-S(＝O)₂NR^a2R^b2、-S(＝O)R^a2and-C (═ NR)^a2)NR^b2R^c2Substituted with the substituent(s);

or, two adjacent R²Together with the C atom to which they are attached form a five-to seven-membered lactam ring, optionally substituted by one or more groups selected from halogen, C_1-6Alkyl substituent substitution;

R³、R⁴、R⁵、R⁶each independently selected from H and R^X2；

Or, R⁴And R⁵Together with the C atom to which they are attached form a five-to seven-membered heterocyclic ring containing 1 or 2 heteroatoms each independently selected from N, O and S, and optionally substituted with one or more substituents selected from halogen and C_1-6Alkyl substituent substitution;

R^X1and R^X2Each occurrence independently selected from halogen, -NO₂、-CN、C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, -OR⁷、-SR⁷、-NR⁷R⁸、-C(＝O)OR⁷、-C(＝O)NR⁷R⁸、-OC(＝O)R⁷、-NC(＝O)R⁷R⁸、-C(＝O)R⁷、-S(＝O)₂OR⁷、-S(＝O)₂R⁷、-S(＝O)₂NR⁷R⁸、-OS(＝O)₂R⁷、-NS(＝O)₂R⁷R⁸、-S(＝O)R⁷Wherein said alkyl, alkenyl or alkynyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -SH, -S (C)_1-6Alkyl), -S (C)_3-6Cycloalkyl), -S (C)_1-4alkylene-C_3-6Cycloalkyl), -S (three-to seven-membered heterocyclyl), -S (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂O, -COOH and C_1-6Alkyl substituent substitution;

R⁷、R⁸each occurrence is independently selected from H, C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl radical, C_6-10aryl-C_1-4Alkyl, wherein the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl or aryl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-COOH、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl) substituted with a substituent;

R^a1、R^b1、R^c1、R^a2、R^b2、R^c2each occurrence is independently selected from H, C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl radical, C_6-10Aryl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, -OR^Y1、-SR^Y1、-NR^Y1R^Y2、-C(＝O)OR^Y1、-C(＝O)NR^Y1R^Y2、-C(＝O)R^Y1、-S(＝O)₂OR^Y1、-S(＝O)₂R^Y1、-S(＝O)₂NR^Y1R^Y2、-S(＝O)R^Y1Wherein said alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl OR heteroaryl is optionally substituted with one OR more substituents selected from halogen, ═ O, ═ S, -OR^Y3、-SR^Y3、-NR^Y3R^Y4、-C(＝O)R^Y3、-C(＝O)OR^Y3and-C (═ O) NR^Y3R^Y4Substituted with the substituent(s);

R^Y1、R^Y2、R^Y3、R^Y4each occurrence is independently selected from H, C_1-8Alkyl radical, C_3-10Cycloalkyl radical, C_3-10Cycloalkyl radical-C_1-4Alkyl, three-to ten-membered heterocyclyl-C_1-4Alkyl radical, C_6-10Aryl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, wherein the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, -OH, -SH, -NH₂Substituted with a substituent of ═ O and-COOH;

n is 1 or 2;

with the proviso that the compound of formula (I) does not include the following structure:

wherein R is³Is selected from-O (C)_1-6Alkyl), -O (C)_1-4alkylene-C_6-10Aryl groups); r¹⁰Is H;

and the compounds of formula (I) do not include the following structure:

wherein R is⁴is-O (C)_1-6Alkyl groups);R⁵is halogen; r¹⁰Is CF₃；

And the compounds of formula (I) do not include the following structure:

wherein R is⁴Is selected from-O (C)_1-6Alkyl), -O (C)_1-4alkylene-C_6-10Aryl groups);

ring C being C_6-10Aryl, optionally substituted with one or more substituents each independently selected from R^X1Substituted with a group of (1);

and the compounds of formula (I) do not include the following structure:

wherein R is⁴Selected from-OH, -O (C)_1-6Alkyl groups); r⁵Is halogen; the C ring is a five-to ten-membered heteroaryl group optionally substituted with one or more substituents each independently selected from R^X1Is substituted with a group (b).

In one embodiment, R¹Is ═ O. In another embodiment, R¹Is ═ S. In another embodiment, R¹Is OR⁷。

In one embodiment, ring B is a saturated or unsaturated five or six membered heterocyclic ring containing 1,2 or 3 heteroatoms each independently selected from N, O and S. In one embodiment, ring B is a saturated or unsaturated five-membered heterocyclic ring containing 1 or 2 heteroatoms each independently selected from N and O. In another embodiment, the B ring is a dihydropyrrole. In another embodiment, the B ring is selected from the group consisting of 2, 3-dihydro-1H-pyrrole and 3, 4-dihydro-1H-pyrrole, preferably 2, 3-dihydro-1H-pyrrole. In yet another embodiment, the B ring is pyrrolidine.

In a more preferred embodiment, the A-B ring system is

Wherein Y is O or S; and ring C is a five-to seven-membered heteroaryl group, preferably a five-to six-membered heteroaryl group, especially a five-membered heteroaryl group, optionally substituted with 1,2, 3,4 or 5 each independently selected from R^X1Is substituted with a group (b). In a particular embodiment, the A-B ring system is

And ring C is a five-to seven-membered heteroaryl group, preferably a five-to six-membered heteroaryl group, especially a five-membered heteroaryl group, optionally substituted with 1,2, 3,4 or 5 each independently selected from R^X1Is substituted with a group (b). In another embodiment, ring C contains 1,2, 3 or 4 heteroatoms each independently selected from N, O and S, preferably from N and O. In yet another embodiment, there is at least one N atom in the C ring. In one embodiment, ring C is a five-membered heteroaryl group containing 1 or 2N atoms, optionally substituted with 1,2, 3,4 or 5 each independently selected from R^X1Is substituted with a group (b). In another embodiment, the C ring is selected from pyrrole and imidazole.

In one embodiment, ring B is a saturated or unsaturated six-membered heterocyclic ring containing 1 or 2 heteroatoms each independently selected from N and O. In another embodiment, the B ring is a dihydropyrimidine. In a preferred embodiment, the B ring is selected from the group consisting of 1, 6-dihydropyrimidine, 1, 2-dihydropyrimidine, 1, 4-dihydropyrimidine.

In a more preferred embodiment, the A-B ring system is

Wherein Y is O or S. In a particular embodiment, the A-B ring system is

In yet another embodiment, the B ring is 2H-pyran or 4H-pyran. In a preferred embodiment, the A-B ring system is

Wherein Y is O or S. In a particular embodiment of the process of the present invention,A-B ring system is

In an alternative embodiment, the B ring is 2H-thiopyran or 4H-thiopyran. In one embodiment, A-B is a ring system

Wherein Y is O or S.

In another alternative embodiment, the B ring is a dihydropyridine. In one embodiment, the B ring is selected from 1, 6-dihydropyridine, 1, 2-dihydropyridine, 1, 4-dihydropyridine. In one embodiment, A-B is a ring system

Wherein Y is O or S.

In yet another alternative embodiment, the A-B ring system is

In another alternative embodiment, the A-B ring system is

In yet another embodiment, ring C is phenyl, optionally substituted with 1,2, 3,4 or 5 each independently selected from R^X1Is substituted with a group (b).

In one embodiment, L¹Is a bond. In another embodiment, L¹Is C₁-C₆A hydrocarbon chain. In a preferred embodiment, L¹Is C₁-C₂A hydrocarbon chain.

In one embodiment, A-B is a ring system

C ring is absent, and L¹Is absent.

In another embodiment:

ring A is a benzene ring;

ring B is a saturated or unsaturated five-or six-membered heterocyclic ring, phenyl ring, or six-membered heteroaromatic ring containing 1,2, or 3 heteroatoms each independently selected from N, O and S;

c ring is selected from C_6-10Aryl and five-to ten-membered heteroaryl, optionally substituted with one or more substituents each independently selected from R^X1Substituted with a group of (1);

L¹is a bond, or is C₁-C₆A hydrocarbon chain;

R¹is ═ Y, where Y is O OR S, OR is OR⁷；

R²At each occurrence is independently selected from H, halogen, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, C_3-10Cycloalkyl radical, C_3-10Cycloalkyl radical-C_1-4Alkyl, three-to ten-membered heterocyclyl-C_1-4Alkyl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, ═ O, ═ S, ═ NR^a1、-OR^a1、-SR^a1、-NR^a1R^b1、-C(＝O)OR^a1、-C(＝O)NR^a1R^b1、-C(＝O)R^a1、-S(＝O)₂OR^a1、-S(＝O)₂R^a1、-S(＝O)₂NR^a1R^b1、-S(＝O)R^a1、-C(＝S)OR^a1、-C(＝S)NR^a1R^b1、-C(＝S)R^a1、-P(＝O)(OR^a1)OR^b1、-C(＝NR^a1)NR^b1R^c1-OCN, -SCN, -N ═ C ═ O, -NCS, where the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl or heteroaryl are optionally substituted with one or more substituents selected from halo, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, ═ O, ═ S, -OR^a2、-SR^a2、-NR^a2R^b2、-C(＝O)OR^a2、-C(＝O)NR^a2R^b2、-C(＝O)R^a2、-S(＝O)₂OR^a2、-S(＝O)₂R^a2、-S(＝O)₂NR^a2R^b2、-S(＝O)R^a2and-C (═ NR)^a2)NR^b2R^c2Substituted with the substituent(s);

R³、R⁴、R⁵、R⁶each independently selected from H and R^X2；

Or, R⁴And R⁵Together with the C atom to which they are attached form a five-to seven-membered heterocyclic ring containing 1 or 2 heteroatoms each independently selected from N, O and S, and optionally substituted with one or more substituents selected from halogen and C_1-6Alkyl substituent substitution;

R^X1and R^X2Each occurrence independently selected from halogen, -NO₂、-CN、C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, -OR⁷、-SR⁷、-NR⁷R⁸、-C(＝O)OR⁷、-C(＝O)NR⁷R⁸、-OC(＝O)R⁷、-NC(＝O)R⁷R⁸、-C(＝O)R⁷、-S(＝O)₂OR⁷、-S(＝O)₂R⁷、-S(＝O)₂NR⁷R⁸、-OS(＝O)₂R⁷、-NS(＝O)₂R⁷R⁸、-S(＝O)R⁷Wherein said alkyl, alkenyl or alkynyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -SH, -S (C)_1-6Alkyl), -S (C)_3-6Cycloalkyl), -S (C)_1-4alkylene-C_3-6Cycloalkyl), -S (three-to seven-membered heterocyclyl), -S (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4Alkylene radical-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂O, -COOH and C_1-6Alkyl substituent substitution;

R⁷、R⁸each occurrence is independently selected from H, C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl radical, C_6-10aryl-C_1-4Alkyl, wherein the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl or aryl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-COOH、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl) substituted with a substituent;

R^a1、R^b1、R^c1、R^a2、R^b2、R^c2each occurrence is independently selected from H, C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl radical, C_6-10Aryl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, -OR^Y1、-SR^Y1、-NR^Y1R^Y2、-C(＝O)OR^Y1、-C(＝O)NR^Y1R^Y2、-C(＝O)R^Y1、-S(＝O)₂OR^Y1、-S(＝O)₂R^Y1、-S(＝O)₂NR^Y1R^Y2、-S(＝O)R^Y1Wherein said alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl OR heteroaryl is optionally substituted with one OR more substituents selected from halogen, ═ O, ═ S, -OR^Y3、-SR^Y3、-NR^Y3R^Y4、-C(＝O)R^Y3、-C(＝O)OR^Y3and-C (═ O) NR^Y3R^Y4Substituted with the substituent(s);

R^Y1、R^Y2、R^Y3、R^Y4each occurrence is independently selected from H, C_1-8Alkyl radical, C_3-10Cycloalkyl radical, C_3-10Cycloalkyl radical-C_1-4Alkyl, three-to ten-membered heterocyclyl-C_1-4Alkyl radical, C_6-10Aryl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, wherein the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, -OH, -SH, -NH₂Substituted with a substituent of ═ O and-COOH;

n is 1;

provided that and the compound of formula (I) does not include the following structure:

wherein R is⁴Is selected from-O (C)_1-6Alkyl), -O (C)_1-4alkylene-C_6-10Aryl groups);

ring C being C_6-10Aryl, optionally substituted with one or more substituents each independently selected from R^X1Substituted with a group of (1);

and the compounds of formula (I) do not include the following structure:

wherein R is⁴Selected from-OH,-O(C_1-6Alkyl groups); r⁵Is halogen; the C ring is a five-to ten-membered heteroaryl group optionally substituted with one or more substituents each independently selected from R^X1Is substituted with a group (b).

The structure described by the following formula (II) falls within the scope of the structure described by the formula (I). In another aspect of the present invention, there is provided a use of a compound of formula (II), or a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite or prodrug thereof, for the preparation of a medicament for the prevention or treatment of a polyQ-related neurodegenerative disease

Wherein:

y is O or S;

the C ring is a five-to seven-membered heteroaryl group optionally substituted with one or more substituents each independently selected from R^X1Substituted with a group of (1);

R²selected from H, C_1-8An alkyl group;

L¹is a bond, or is C₁-C₆A hydrocarbon chain;

R³、R⁴、R⁵、R⁶each independently selected from H and R^X2；

Wherein R is^X1、R^X2As defined in formula (I).

In one embodiment, Y is O. When Y is O, formula (II) is

In one embodiment, the C ring is a five to six membered heteroaryl group, optionally substituted with 1,2, 3,4 or 5 each independently selected from R^X1Is substituted with a group (b). In a preferred embodiment, the C ring is a five membered heteroaryl group optionally substituted with 1,2, 3,4 or 5 each independently selected from R^X1Is substituted with a group (b). In another embodiment, the C ring contains 1,2, 3 or4 heteroatoms, each independently selected from N, O and S, preferably from N and O. In yet another embodiment, there is at least one N atom in the C ring. In one embodiment, ring C is a five-membered heteroaryl group containing 1 or 2N atoms, optionally substituted with 1,2, 3,4 or 5 each independently selected from R^X1Is substituted with a group (b). In another embodiment, the C ring is selected from pyrrole and imidazole.

In one embodiment, L¹Is a bond. In another embodiment, L¹Is C₁-C₆A hydrocarbon chain. In a preferred embodiment, L¹Is C₁-C₂A hydrocarbon chain. In one embodiment, L¹Is methylene or methine. In a particular embodiment, L¹Is methine. In another particular embodiment, L¹Is composed of

In another particular embodiment, the compound of formula (II) is selected from:

the structure described by the following formula (III) falls within the scope of the structure described by the formula (I). In another aspect of the present invention, there is provided a use of a compound of formula (III), or a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite or prodrug thereof, for the preparation of a medicament for the prevention or treatment of a polyQ-related neurodegenerative disease

Wherein:

ring B is a saturated or unsaturated six-membered heterocyclic, phenyl or heteroaryl ring containing 1,2 or 3 heteroatoms each independently selected from N, O and S;

ring C being C_6-10Aryl, optionally substituted with one or more substituents each independently selected from R^X1Substituted with a group of (1);

or, C ring is absent, and L¹Is absent;

ring A, ring L¹、R¹、R²、R³、R⁴、R⁵、R⁶、R^X1N is as defined in formula (I);

with the proviso that the compound of formula (III) does not include the following structure:

wherein R is³Is selected from-O (C)_1-6Alkyl), -O (C)_1-4alkylene-C_6-10Aryl groups); r¹⁰Is H;

and the compounds of formula (III) do not include the following structure:

wherein R is⁴is-O (C)_1-6Alkyl groups); r⁵Is halogen; r¹⁰Is CF₃；

And the compounds of formula (III) do not include the following structure:

wherein R is⁴Is selected from-O (C)_1-6Alkyl), -O (C)_1-4alkylene-C_6-10Aryl groups);

ring C being C_6-10Aryl, optionally substituted with one or more substituents each independently selected from R^X1Substituted with a group of (1);

and the compounds of formula (III) do not include the following structure:

wherein R is⁴Selected from-OH, -O (C)_1-6Alkyl groups); r⁵Is halogen; the C ring is a five-to ten-membered heteroaryl group optionally substituted with one or more substituents each independently selected from R^X1Is substituted with a group (b).

In one embodiment, ring B is a saturated or unsaturated six-membered heterocyclic ring containing 1,2, or 3 heteroatoms each independently selected from N, O and S. In one embodiment, ring B is a saturated or unsaturated six-membered heterocyclic ring containing 1 or 2 heteroatoms each independently selected from N and O. In another embodiment, the B ring is a dihydropyrimidine. In a preferred embodiment, the B ring is selected from the group consisting of 1, 6-dihydropyrimidine, 1, 2-dihydropyrimidine, 1, 4-dihydropyrimidine.

In a more preferred embodiment, the A-B ring system is

Wherein Y is O or S. In a particular embodiment, the A-B ring system is

In yet another embodiment, the B ring is 2H-pyran or 4H-pyran. In a preferred embodiment, the A-B ring system is

Wherein Y is O or S. In a particular embodiment, the A-B ring system is

In an alternative embodiment, the B ring is 2H-thiopyran or 4H-thiopyran. In one embodiment, A-B is a ring system

Wherein Y is O or S.

In another alternative embodiment, the B ring is a dihydropyridine. In one embodiment, the B ring is selected from 1,6-Dihydropyridines, 1, 2-dihydropyridines, 1, 4-dihydropyridines. In one embodiment, A-B is a ring system

Wherein Y is O or S.

In yet another alternative embodiment, the A-B ring system is

In another alternative embodiment, the A-B ring system is

In yet another embodiment, ring C is phenyl, optionally substituted with 1,2, 3,4 or 5 each independently selected from R^X1Is substituted with a group (b).

In one embodiment, L¹Is a bond. In another embodiment, L¹Is C₁-C₆A hydrocarbon chain. In a preferred embodiment, L¹Is C₁-C₂A hydrocarbon chain.

In one embodiment, A-B is a ring system

C ring is absent, and L¹Is absent.

In an alternative embodiment, the B and C rings of the compound of formula (III) are further defined by L²Linked to give a variant of the compound of formula (III) having the structure of formula (III') below

Wherein:

ring C being C_6-10Aryl, optionally substituted with one or more substituents each independently selected from R^X1Substituted with a group of (1);

R¹is H, ═ O, OR is OR⁷；

L¹Is a bond, or is C₁-C₂A hydrocarbon chain;

L²is a bond, or is C₁-C₂A hydrocarbon chain;

provided that L is¹And L²Not being a bond at the same time;

R³、R⁴、R⁵、R⁶each independently selected from H and R^X2；

Ring A, ring B, ring R²、n、R^X1As defined in formula (III).

In one embodiment, R¹Is H.

In another embodiment, R²is-OH.

In one embodiment, R^X2Each occurrence independently selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl groups).

The structures described by the following formula (IV) fall within the scope of the structures described by the formulae (I) and (III). In another aspect of the present invention, there is provided a use of a compound of formula (IV), or a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite or prodrug thereof, for the preparation of a medicament for the prevention or treatment of a polyQ-related neurodegenerative disease

Wherein:

y is O or S;

x is O, NH or S;

R⁹selected from H, halogen, C_1-6Alkyl radical, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl, -OR^a1、-SR^a1、-NR^a1R^b1、-C(＝O)OR^a1、-C(＝O)NR^a1R^b1、-C(＝O)R^a1、-S(＝O)₂OR^a1、-S(＝O)₂R^a1、-S(＝O)₂NR^a1R^b1、-S(＝O)R^a1Wherein said alkyl, cycloalkyl or heterocyclyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OR^a2、-SR^a2、-NR^a2R^b2、-C(＝O)OR^a2、-C(＝O)NR^a2R^b2、-C(＝O)R^a2、-S(＝O)₂OR^a2、-S(＝O)₂R^a2、-S(＝O)₂NR^a2R^b2and-S (═ O) R^a2Substituted with the substituent(s); wherein R is^a1、R^b1、R^a2、R^b2As defined in formula (III);

R¹⁰selected from H, halogen, C_1-6Alkyl radical, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4An alkyl group;

or, R⁹And R¹⁰Together with the C atom to which they are attached form a five-to seven-membered lactam ring, optionally substituted by one or more groups selected from halogen, C_1-6Alkyl substituent substitution;

R³selected from H, halogen, C_1-6Alkyl, -OH, -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Wherein said alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Substituted with the substituent(s);

R⁴selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OR⁷、-SR⁷、-NR⁷R⁸；R⁷、R⁸Each occurrence is independently selected from H, C_1-6Alkyl, wherein the alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-COOH、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl) substituted with a substituent; wherein R is⁷、R⁸As defined in formula (III);

R⁵selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl), wherein the alkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl) substituted with a substituent;

or, R⁴And R⁵Together with the C atom to which they are attached form a five-to seven-membered heterocyclic ring containing 1 or 2 heteroatoms each independently selected from N, O and S, and optionally substituted with one or more substituents selected from halogen and C_1-6Alkyl substituent substitution;

R⁶selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -O (benzyl), -SH, -S (C)_1-6Alkyl), -S (benzyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂-NH (benzyl), wherein said alkyl or benzyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Substituted with the substituent(s);

provided that when R is⁴is-O (C)_1-6Alkyl) and R⁵When it is halogen, R¹⁰Is not CF₃。

In one embodiment, Y is O. When Y is O, formula (IV) is

In one embodiment, Y is O and X is N, formula (IV) is

In one embodiment, Y is O and X is S, formula (IV) is

In one embodiment, R⁹Selected from H, halogen, C_1-6Alkyl, -OR^a1、-NR^a1R^b1、-C(＝O)OR^a1、-C(＝O)NR^a1R^b1、-S(＝O)₂NR^a1R^b1Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OR^a2、-SR^a2、-NR^a2R^b2、-C(＝O)OR^a2、-C(＝O)NR^a2R^b2、-C(＝O)R^a2Is substituted with the substituent(s). In one embodiment, R⁹Selected from H, halogen, -OR^a1、-NR^a1R^b1、-C(＝O)OR^a1、-C(＝O)NR^a1R^b1. In one embodiment, R⁹is-OR^a1or-NR^a1R^b1. In another embodiment, R⁹is-C (═ O) OR^a2or-C (═ O) NR^a2R^b2. In a particular embodiment, R⁹is-NHC (═ O) (C)_1-6Alkyl groups). In a particular embodiment, R⁹is-NHC (═ O) CH₃。

In one embodiment, R¹⁰Selected from H, halogen or methyl. In another embodiment, R¹⁰Is H.

In one embodiment, R⁹And R¹⁰Together with the C atom to which it is attached, form a six-membered lactam ring. In another embodiment, formula (IV) is

In which R is¹¹Selected from halogen, C_1-6An alkyl group; p is 0,1 or 2. In one embodiment, X and Y are O, formula (IV) is

In which R is¹¹Selected from halogen, C_1-6An alkyl group; p is 0,1 or 2.

In one embodiment, R^a1、R^b1Each occurrence is independently selected from H, C_1-6Alkyl, -C (═ O) R^Y1Wherein said alkyl is optionally substituted by one OR more groups selected from halogen, -OR^Y3、-SR^Y3、-NR^Y3R^Y4、-C(＝O)R^Y3、-C(＝O)OR^Y3and-C (═ O) NR^Y3R^Y4Is substituted with the substituent(s). Wherein R is^Y1、R^Y3、R^Y4As defined in formula (III). In one embodiment, R^a1、R^b1Each occurrence is independently selected from H, C_1-6Alkyl, -C (═ O) (C)_1-6Alkyl), wherein the alkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -O (C)_1-6Alkyl), -O (C)_3-6Cycloalkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-C(＝O)(C_1-6Alkyl), -C (═ O) O (C)_1-6Alkyl), -C (═ O) O (C)_3-6Cycloalkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R^a1、R^b1Independently at each occurrenceSelected from H and C_1-6An alkyl group.

In one embodiment, R^a2、R^b2Each occurrence is independently selected from H, C_1-6An alkyl group.

In one embodiment, R³Selected from H, -OH, C_1-6Alkyl, wherein the alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-SH、-NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂And a substituent of-COOH. In one embodiment, R³Selected from H, halogen, methyl, -OH, -NH₂、-NHCH₃Wherein said methyl is optionally substituted by a group selected from halogen, -OH, -NH₂、-NH(C_1-2Alkyl) groups. In one embodiment, R³Selected from H, halogen, methyl, -OH, -NH₂、-NHCH₃Wherein said methyl is optionally substituted by a substituent selected from the group consisting of halogen, -OH, -OCH₃、-NH₂、-NHCH₃Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, methyl, -OH, -NH₂. In one embodiment, R³Selected from H, F, Cl, methyl, -OH, -NH₂. In another embodiment, R³Selected from H, F, methyl, -OH, -NH₂. In a particular embodiment, R³Selected from H, methyl, -OH. In another particular embodiment, R³Is H. In yet another particular embodiment, R³is-OH. In another embodiment, R³Is a dimethylaminomethyl group.

In one embodiment, R⁴Selected from H, -OR⁷、-SR⁷、-NR⁷R⁸；R⁷、R⁸Each occurrence is independently selected from H, C_1-6Alkyl, wherein said alkyl is optionally substituted by one or more groups selected from halogen, C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-COOH、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R⁴Is C_1-6Alkyl, wherein said alkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -COOH, -C (═ O) O (C)_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s). In a particular embodiment, R⁴is-CH₂COOH. In another embodiment, R⁴Selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Wherein said alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R⁴Selected from H, halogen, C_1-3Alkyl, -OH, -O (C)_1-3Alkyl), -NH₂、-NH(C_1-3Alkyl), -N (C)_1-3Alkyl radical)₂and-COOH, wherein said alkyl is optionally substituted with one or more groups selected from halogen, -OH, -O (C)_1-2Alkyl), -NH₂、-NH(C_1-2Alkyl), -N (C)_1-2Alkyl radical)₂And a substituent of-COOH.

In one embodiment, R⁵Selected from H, C_1-6Alkyl, wherein the alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -SH, -S (C)_1-6Alkyl radical)、-S(C_3-6Cycloalkyl), -S (C)_1-4alkylene-C_3-6Cycloalkyl), -S (three-to seven-membered heterocyclyl), -S (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂And a substituent of-COOH. In another embodiment, R⁵Selected from H, halogen, C_1-3Alkyl, -OH, -O (C)_1-3Alkyl), -NH₂、-NH(C_1-3Alkyl), -N (C)_1-3Alkyl radical)₂and-COOH, wherein said alkyl is optionally substituted with one or more groups selected from halogen, -OH, -O (C)_1-2Alkyl), -NH₂、-NH(C_1-2Alkyl), -N (C)_1-2Alkyl radical)₂And a substituent of-COOH. In one embodiment, R⁵Selected from H, halogen, C_1-3Alkyl, -OH, -O (C)_1-3Alkyl), -NH₂、-NH(C_1-3Alkyl), -N (C)_1-3Alkyl radical)₂Wherein said alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-2Alkyl), -NH₂、-NH(C_1-2Alkyl), -N (C)_1-2Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R⁵Is a dimethylaminomethyl group.

In one embodiment, R⁴And R⁵Together with the C atom to which they are attached form a five-to seven-membered heterocyclic ring containing 1 or 2 heteroatoms each independently selected from N, O and S, including at least one N atom, and optionally substituted with one or more substituents selected from halogen and C_1-6Alkyl, preferably said heterocycle is optionally substituted with one or more methyl or ethyl groups. In one embodiment, R⁴And R⁵Together with the C atom to which they are attached form a five-to seven-membered heterocyclic ring containing 1N atom and optionally substituted by one or more methyl or ethyl groups. In one embodiment, R⁴And R⁵Together with the C atom to which they are attached form a five-to seven-membered heterocyclic ring which includes one N atom, and formula (IV) is

In which R is¹²Selected from H, methyl and ethyl; r¹³Selected from halogen, C_1-6An alkyl group; q is 0,1, 2 or 3. In one embodiment, X and Y are O, formula (IV) is

In which R is¹²Selected from H, methyl and ethyl; r¹³Selected from halogen, C_1-6An alkyl group; q is 0,1, 2 or 3.

In one embodiment, R⁶Selected from H, halogen, C_1-6Alkyl, -OH, -NH₂Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -OH, -NH₂Is substituted with the substituent(s). In one embodiment, R⁶Selected from H, F, Cl, Br, C_1-6Alkyl, -OH, -NH₂Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -OH, -NH₂Is substituted with the substituent(s). In one embodiment, R⁶Selected from H, F, Cl, Br, methyl, -OH, -NH₂. In one embodiment, R⁶Is H or-OH.

In one embodiment, R³Is methyl, and R⁴Selected from-OH, -NH₂、-NH(C_1-3Alkyl), -N (C)_1-3Alkyl radical)₂C substituted by-COOH_1-3Alkyl, wherein said substituted C_1-3Alkyl is substituted by one or more groups selected from-OH, -NH₂、-NH(C_1-2Alkyl), -N (C)_1-2Alkyl radical)₂And a substituent of-COOH. In one embodiment, R³Is methyl, and R⁴Is selected from-NH₂、-NH(C_1-3Alkyl), -N (C)_1-3Alkyl radical)₂C substituted by-COOH_1-3Alkyl, wherein said substituted C_1-3Alkyl is substituted by one or more groups selected from-OH, -NH₂、-NH(C_1-2Alkyl), -N (C)_1-2Alkyl radical)₂And a substituent of-COOH.

In another embodiment, R³Is methyl, and R⁵Selected from-OH, -NH₂、-NH(C_1-3Alkyl), -N (C)_1-3Alkyl radical)₂C substituted by-COOH_1-3Alkyl, wherein said substituted C_1-3Alkyl is substituted by one or more groups selected from-OH, -NH₂、-NH(C_1-2Alkyl), -N (C)_1-2Alkyl radical)₂And a substituent of-COOH. In another embodiment, R³Is methyl, and R⁵Is selected from-NH₂、-NH(C_1-3Alkyl), -N (C)_1-3Alkyl radical)₂C substituted by-COOH_1-3Alkyl, wherein said substituted C_1-3Alkyl is substituted by one or more groups selected from-OH, -NH₂、-NH(C_1-2Alkyl), -N (C)_1-2Alkyl radical)₂And a substituent of-COOH.

The structures described by the following formula (V) fall within the scope of the structures described by formula (I), formula (III) and formula (IV). In another aspect of the present invention, there is provided a use of a compound of formula (V), or a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite or prodrug thereof, for the preparation of a medicament for the prevention or treatment of a polyQ-related neurodegenerative disease

Wherein:

Y、R⁹、R¹⁰、R³、R⁴、R⁵、R⁶as defined in formula (IV);

in one embodiment, Y is O. When Y is O, formula (V) is

In a particular embodiment, the compound of formula (V) is

The structures described by the following formula (VI) fall within the scope of the structures described by the formulae (I) and (III). In another aspect of the present invention, there is provided a use of a compound of formula (VI), or a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite or prodrug thereof, for the preparation of a medicament for the prevention or treatment of a polyQ-related neurodegenerative disease

Wherein:

ring B is a saturated or unsaturated six-membered heterocyclic ring containing 1,2, or 3 heteroatoms each independently selected from N, O and S;

ring C being C_6-10Aryl, optionally substituted with one or more substituents each independently selected from R^X1Substituted with a group of (1);

L¹is a bond, or is C₁-C₆A hydrocarbon chain;

R²selected from H, halogen, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, C_3-10Cycloalkyl radical, C_3-10Cycloalkyl radical-C_1-4Alkyl, three-to ten-membered heterocyclyl-C_1-4Alkyl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, ═ O, ═ S, ═ NR^a1、-OR^a1、-SR^a1、-NR^a1R^b1、-C(＝O)OR^a1、-C(＝O)NR^a1R^b1、-C(＝O)R^a1、-S(＝O)₂OR^a1、-S(＝O)₂R^a1、-S(＝O)₂NR^a1R^b1、-S(＝O)R^a1、-C(＝S)OR^a1、-C(＝S)NR^a1R^b1、-C(＝S)R^a1、-P(＝O)(OR^a1)OR^b1、-C(＝NR^a1)NR^b1R^c1-OCN, -SCN, -N ═ C ═ O, -NCS, where the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl or heteroaryl are optionally substituted with one or more substituents selected from halo, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, ═ O, ═ S, -OR^a2、-SR^a2、-NR^a2R^b2、-C(＝O)OR^a2、-C(＝O)NR^a2R^b2、-C(＝O)R^a2、-S(＝O)₂OR^a2、-S(＝O)₂R^a2、-S(＝O)₂NR^a2R^b2、-S(＝O)R^a2and-C (═ NR)^a2)NR^b2R^c2Substituted with the substituent(s);

R³、R⁴、R⁵、R⁶each independently selected from H and R^X2；

Or, R⁴And R⁵Together with the C atom to which they are attached form a five-to seven-membered heterocyclic ring containing 1 or 2 heteroatoms each independently selected from N, O and S, and optionally substituted with one or more substituents selected from halogen and C_1-6Alkyl substituent substitution;

R^X1and R^X2Each occurrence independently selected from halogen, -NO₂、-CN、C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, -OR⁷、-SR⁷、-NR⁷R⁸、-C(＝O)OR⁷、-C(＝O)NR⁷R⁸、-OC(＝O)R⁷、-NC(＝O)R⁷R⁸、-C(＝O)R⁷、-S(＝O)₂OR⁷、-S(＝O)₂R⁷、-S(＝O)₂NR⁷R⁸、-OS(＝O)₂R⁷、-NS(＝O)₂R⁷R⁸、-S(＝O)R⁷Wherein said alkyl, alkenyl or alkynyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -SH, -S (C)_1-6Alkyl), -S (C)_3-6Cycloalkyl), -S (C)_1-4alkylene-C_3-6Cycloalkyl), -S (three-to seven-membered heterocyclyl), -S (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂O, -COOH and C_1-6Alkyl substituent substitution;

R⁷、R⁸each occurrence is independently selected from H, C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl, wherein the alkyl, alkenyl, alkynyl, cycloalkyl or heterocyclyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-COOH、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ n)O)(C_1-6Alkyl), -C (═ O) (C)_1-6Alkyl) substituted with a substituent;

R^a1、R^b1、R^c1、R^a2、R^b2、R^c2each occurrence is independently selected from H, C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl radical, C_6-10Aryl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, -OR^Y1、-SR^Y1、-NR^Y1R^Y2、-C(＝O)OR^Y1、-C(＝O)NR^Y1R^Y2、-C(＝O)R^Y1、-S(＝O)₂OR^Y1、-S(＝O)₂R^Y1、-S(＝O)₂NR^Y1R^Y2、-S(＝O)R^Y1Wherein said alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl OR heteroaryl is optionally substituted with one OR more substituents selected from halogen, ═ O, ═ S, -OR^Y3、-SR^Y3、-NR^Y3R^Y4、-C(＝O)R^Y3、-C(＝O)OR^Y3and-C (═ O) NR^Y3R^Y4Substituted with the substituent(s);

R^Y1、R^Y2、R^Y3、R^Y4each occurrence is independently selected from H, C_1-8Alkyl radical, C_3-10Cycloalkyl radical, C_3-10Cycloalkyl radical-C_1-4Alkyl, three-to ten-membered heterocyclyl-C_1-4Alkyl radical, C_6-10Aryl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, wherein the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, -OH, -SH, -NH₂Substituted with a substituent of ═ O and-COOH;

a ring, R¹As defined in formula (III);

with the proviso that the compound of formula (VI) does not include the following structure:

wherein R is⁴Is selected from-O (C)_1-6Alkyl), -O (C)_1-4alkylene-C_6-10Aryl).

In one embodiment, ring B is a saturated or unsaturated six-membered heterocyclic ring containing 1 or 2 heteroatoms each independently selected from N and O. In another embodiment, the B ring is a dihydropyrimidine. In a preferred embodiment, the B ring is selected from the group consisting of 1, 6-dihydropyrimidine, 1, 2-dihydropyrimidine, 1, 4-dihydropyrimidine.

In a more preferred embodiment, the A-B ring system is

Wherein Y is O or S. In a particular embodiment, the A-B ring system is

In yet another embodiment, the B ring is 2H-pyran or 4H-pyran. In a preferred embodiment, the A-B ring system is

Wherein Y is O or S. In a particular embodiment, the A-B ring system is

In yet another embodiment, ring C is phenyl, optionally substituted with 1,2, 3,4 or 5 each independently selected from R^X1Is substituted with a group (b).

In one embodiment, L¹Is a bond. In another embodiment, L¹Is C₁-C₆A hydrocarbon chain. In a preferred embodiment, L¹Is C₁-C₂A hydrocarbon chain.

In yet another embodiment, R²Selected from H, halogen, -NO₂、-CN、C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl, ═ O, ═ S, -OR^a1、-SR^a1、-NR^a1R^b1、-C(＝O)OR^a1、-C(＝O)NR^a1R^b1、-C(＝O)R^a1、-S(＝O)₂OR^a1、-S(＝O)₂R^a1、-S(＝O)₂NR^a1R^b1、-S(＝O)R^a1Wherein said alkyl, alkenyl, alkynyl, cycloalkyl or heterocyclyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OR^a2、-SR^a2、-NR^a2R^b2、-C(＝O)OR^a2、-C(＝O)NR^a2R^b2、-C(＝O)R^a2、-S(＝O)₂OR^a2、-S(＝O)₂R^a2、-S(＝O)₂NR^a2R^b2and-S (═ O) R^a2Is substituted with the substituent(s). In another embodiment, R²Selected from H, halogen, -NO₂、-CN、＝O、＝S、C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl, -OH, -O (C)_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclic group), -O (C ═ O) (C)_1-6Alkyl), -O (C ═ O) (C)_3-6Cycloalkyl), -O (C ═ O) (C)_1-4alkylene-C_3-6Cycloalkyl), -O (C ═ O) (three-to seven-membered heterocyclic group), -O (C ═ O) (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -SH, -S (C)_1-6Alkyl), -S (C)_3-6Cycloalkyl), -S (C)_1-4alkylene-C_3-6Cyclic hydrocarbyl), -S (ternary)To seven membered heterocyclic group), -S (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂、-NH(C＝O)(C_1-6Alkyl), -NH (C ═ O) (C)_3-6Cycloalkyl), -NH (C ═ O) (C)_1-4alkylene-C_3-6Cycloalkyl), -NH (C ═ O) (three-to seven-membered heterocyclyl), -C (═ O) (C)_1-6Alkyl), -COOH, -C (═ O) O (C)_1-6Alkyl), -C (═ O) O (C)_3-6Cycloalkyl), -C (═ O) O (C)_1-4alkylene-C_3-6Cycloalkyl), -C (═ O) O (three-to seven-membered heterocyclyl), -C (═ O) O (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -C (═ O) NH₂、-C(＝O)NH(C_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-C(＝O)NH(C_1-4alkylene-C_3-6Cycloalkyl), -C (═ O) N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂-C (═ O) NH (three-to seven-membered heterocyclic group), -C (═ O) N (three-to seven-membered heterocyclic group)₂、-C(＝O)NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -C (═ O) N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂Wherein said alkyl, alkylene, alkenyl, alkynyl, cycloalkyl or heterocyclyl is optionally substituted with one or more substituents selected from halogen, nitro, cyano, -OH, -SH, -NH₂And a substituent of-COOH. In yet another embodiment, R²Selected from H, halogen, -NO₂、-CN、C_1-6Alkyl radical, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl, -OR^a1、-SR^a1、-NR^a1R^b1、-C(＝O)OR^a1、-C(＝O)NR^a1R^b1、-C(＝O)R^a1、-S(＝O)₂OR^a1、-S(＝O)₂R^a1、-S(＝O)₂NR^a1R^b1、-S(＝O)R^a1Wherein said alkyl, cycloalkyl or heterocyclyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OR^a2、-SR^a2、-NR^a2R^b2、-C(＝O)OR^a2、-C(＝O)NR^a2R^b2、-C(＝O)R^a2Is substituted with the substituent(s). In a preferred embodiment, R²Selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, wherein said alkyl is optionally substituted by one or more groups selected from halogen, -NO₂、-CN、C_1-6Alkyl, -NR^a2R^b2Is substituted with the substituent(s). In a more preferred embodiment, R²Selected from H, halogen, C_1-6Alkyl, -OH, wherein the alkyl is optionally substituted by one or more groups selected from halogen, C_1-6Alkyl and-NR^a2R^b2Is substituted with a group (b). In a further embodiment, R²Selected from H, C_1-4Alkyl, -OH, wherein the alkyl is optionally substituted by one or more groups selected from-NR^a2R^b2Is substituted with a group (b). In a particular embodiment, R²Selected from H, C_1-4Alkyl, -OH, wherein the alkyl is-CH₂[CH(CH₃)₂]And optionally substituted by one or more groups selected from-NR^a2R^b2Is substituted with a group (b). In another embodiment, R²Is represented by-NR^a1R^b1A substituted alkyl group. In a particular embodiment, R²Is composed of

In one embodiment, R⁴And R⁵Together with the C atom to which they are attached form a five-to seven-membered heterocyclic ring containing 1 or 2 heteroatoms each independently selected from N, O and S, including at least one N atom, and optionally being substituted by one or more substituentsFrom halogen and C_1-6Alkyl, preferably said heterocycle is optionally substituted with one or more methyl or ethyl groups. In one embodiment, R⁴And R⁵Together with the C atom to which they are attached form a five-to seven-membered heterocyclic ring containing 1N atom and optionally substituted by one or more methyl or ethyl groups.

In one embodiment, R^X1And R^X2Each occurrence independently selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OR⁷、-SR⁷、-NR⁷R⁸、-C(＝O)OR⁷、-C(＝O)NR⁷R⁸、-OC(＝O)R⁷、-NC(＝O)R⁷R⁸、-C(＝O)R⁷、-S(＝O)₂OR⁷、-S(＝O)₂R⁷、-S(＝O)₂NR⁷R⁸、-OS(＝O)₂R⁷、-NS(＝O)₂R⁷R⁸、-S(＝O)R⁷Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -SH, -S (C)_1-6Alkyl), -S (C)_3-6Cycloalkyl), -S (C)_1-4alkylene-C_3-6Cycloalkyl), -S (three-to seven-membered heterocyclyl), -S (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂Substituted by a substituent of O or COOH. In a preferred embodiment, R^X1And R^X2Each occurrence independently selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OR⁷、-SR⁷、-NR⁷R⁸、-OC(＝O)R⁷、-NC(＝O)R⁷R⁸、-OS(＝O)₂R⁷、-NS(＝O)₂R⁷R⁸Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂Is substituted with the substituent(s). In a preferred embodiment, R^X1And R^X2Independently at each occurrence, is selected from halogen, C_1-6Alkyl, -OR⁷、-NR⁷R⁸More preferably each independently selected from halogen, -OR⁷Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s). In a more preferred embodiment, R^X1And R^X2Each occurrence is independently selected from F, Cl, Br, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Optionally substituted C_1-6Alkyl, wherein said optionally substituted C_1-6Alkyl is optionally substituted by one or more groups selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s). In a particular embodiment, R^X1And R^X2Each occurrence is independently selected from F, Cl, Br, methyl, -OH, dimethylaminomethyl.

R⁷、R⁸Each occurrence is independently selected from H, C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, wherein said alkyl, alkenyl, alkynyl is optionally substituted with one or more substituents selected from halo, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-COOH、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl) groups. In a preferred embodiment, R⁷、R⁸Each occurrence is independently selected from H, C_1-6Alkyl, wherein said alkyl is optionally substituted by one or more groups selected from halogen, C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-COOH、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl) groups. In a particular embodiment, R⁷、R⁸Each occurrence is independently selected from H, C_1-6Alkyl, wherein the alkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -N (C)_1-6Alkyl radical)₂and-COOH. In another particular embodiment, R⁷、R⁸Each occurrence is independently selected from H, C_1-6Alkyl, wherein the alkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s).

In a real worldIn the embodiment, R^a1、R^b1、R^c1、R^a2、R^b2、R^c2Each occurrence is independently selected from H, C_1-6Alkyl radical, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, -OR^Y1、-SR^Y1、-NR^Y1R^Y2、-C(＝O)OR^Y1、-C(＝O)NR^Y1R^Y2、-C(＝O)R^Y1Wherein said alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl OR heteroaryl is optionally substituted with one OR more substituents selected from halogen, -OR^Y3、-SR^Y3、-NR^Y3R^Y4、-C(＝O)R^Y3、-C(＝O)OR^Y3and-C (═ O) NR^Y3R^Y4Is substituted with the substituent(s). In a preferred embodiment, R^a1、R^b1、R^c1、R^a2、R^b2、R^c2Each occurrence is independently selected from H, C_1-6Alkyl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, -OR^Y1、-SR^Y1、-NR^Y1R^Y2、-C(＝O)OR^Y1、-C(＝O)NR^Y1R^Y2、-C(＝O)R^Y1Wherein said alkyl, aryl OR heteroaryl is optionally substituted by one OR more groups selected from halogen, -OR^Y3and-NR^Y3R^Y4Is substituted with the substituent(s). In another preferred embodiment, R^a1、R^b1、R^c1、R^a2、R^b2、R^c2Each occurrence is independently selected from H, C_1-6Alkyl, -OR^Y1、-NR^Y1R^Y2、-C(＝O)OR^Y1、-C(＝O)NR^Y1R^Y2、-C(＝O)R^Y1Wherein said alkyl is optionally substituted by one OR more groups selected from halogen, -OR^Y3and-NR^Y3R^Y4Is substituted with a group (b). In a more preferred embodiment, R^a1、R^b1、R^c1、R^a2、R^b2、R^c2Each occurrence is independently selected from H, C_1-6Alkyl, -OR^Y1、-NR^Y1R^Y2、-C(＝O)R^Y1Wherein said alkyl is optionally substituted by one OR more groups selected from halogen, -OR^Y3and-NR^Y3R^Y4Is substituted with a group (b). In a further embodiment, R^a1、R^b1、R^c1、R^a2、R^b2、R^c2Each occurrence is independently selected from H, C_1-6Alkyl, -OR^Y1、-NR^Y1R^Y2、-C(＝O)R^Y1Wherein said alkyl is optionally substituted by one or more substituents selected from halogen, -NR^Y3R^Y4Is substituted with a group (b). In a particular embodiment, R^a1、R^b1、R^c1、R^a2、R^b2、R^c2Each occurrence is independently selected from H, C_1-3Alkyl, -OH, p-methylbenzoyl; wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NH₂Is substituted with a group (b).

In another embodiment, R^Y1、R^Y2、R^Y3、R^Y4Each occurrence is independently selected from H, C_1-8Alkyl radical, C_3-10Cycloalkyl radical, C_3-10Cycloalkyl radical-C_1-4Alkyl, three-to ten-membered heterocyclyl-C_1-4Alkyl radical, C_6-10Aryl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, wherein the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-SH、-NH₂-COOH and C_1-6Alkyl substituents. In a preferred embodiment, R^Y1、R^Y2、R^Y3、R^Y4Each occurrence is independently selected from H, C_1-6Alkyl radical, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclic groupheterocyclyl-C_1-4Alkyl, phenyl-C_1-4Alkyl, five-to six-membered heteroaryl-C_1-4Alkyl, wherein the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-SH、-NH₂-COOH and C_1-6Alkyl substituents. In a more preferred embodiment, R^Y1、R^Y2、R^Y3、R^Y4Each occurrence is independently selected from H, C_1-6Alkyl, phenyl-C_1-4Alkyl, five-to six-membered heteroaryl-C_1-4Alkyl, wherein the alkyl or phenyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-SH、-NH₂-COOH and C_1-6Alkyl substituents. In a more preferred embodiment, R^Y1、R^Y2、R^Y3、R^Y4Each occurrence is independently selected from H, C_1-6Alkyl, phenyl-C_1-4Alkyl, wherein the alkyl or phenyl is optionally substituted by one or more groups selected from halogen, C_1-6Alkyl groups. In a particular embodiment, R^Y1、R^Y2、R^Y3、R^Y4Each occurrence is independently selected from H and p-methylphenyl.

In another embodiment, R²Selected from H, halogen, -NO₂、-CN、＝O、＝S、C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl, -OH, -O (C)_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclic group), -O (C ═ O) (C)_1-6Alkyl), -O (C ═ O) (C)_3-6Cycloalkyl), -O (C ═ O) (C)_1-4alkylene-C_3-6Cycloalkyl), -O (C ═ O) (three-to seven-membered heterocyclyl),-O(C＝O)(C_1-4Alkylene) - (three-to seven-membered heterocyclyl), -SH, -S (C)_1-6Alkyl), -S (C)_3-6Cycloalkyl), -S (C)_1-4alkylene-C_3-6Cycloalkyl), -S (three-to seven-membered heterocyclyl), -S (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂、-NH(C＝O)(C_1-6Alkyl), -NH (C ═ O) (C)_3-6Cycloalkyl), -NH (C ═ O) (C)_1-4alkylene-C_3-6Cycloalkyl), -NH (C ═ O) (three-to seven-membered heterocyclyl), -C (═ O) (C)_1-6Alkyl), -C (═ O) O (C)_1-6Alkyl), -C (═ O) O (C)_3-6Cycloalkyl), -C (═ O) O (C)_1-4alkylene-C_3-6Cycloalkyl), -C (═ O) O (three-to seven-membered heterocyclyl), -C (═ O) O (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-C(＝O)NH(C_1-4alkylene-C_3-6Cycloalkyl), -C (═ O) N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂-C (═ O) NH (three-to seven-membered heterocyclic group), -C (═ O) N (three-to seven-membered heterocyclic group)₂、-C(＝O)NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -C (═ O) N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂Wherein said alkyl, alkylene, alkenyl, alkynyl, cycloalkyl or heterocyclyl is optionally substituted with one or more substituents selected from halogen, nitro, cyano, -OH, -SH, -NH₂Is substituted with the substituent(s). In yet another embodiment, R²Selected from H, halogen, -NO₂、-CN、C_1-6Alkyl radical, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclic groupThree-to seven-membered heterocyclyl-C_1-4Alkyl, -OR^a1、-SR^a1、-NR^a1R^b1、-C(＝O)OR^a1、-C(＝O)NR^a1R^b1、-C(＝O)R^a1、-S(＝O)₂OR^a1、-S(＝O)₂R^a1、-S(＝O)₂NR^a1R^b1、-S(＝O)R^a1Wherein said alkyl, cycloalkyl or heterocyclyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OR^a2、-SR^a2、-NR^a2R^b2、-C(＝O)OR^a2、-C(＝O)NR^a2R^b2、-C(＝O)R^a2Is substituted with the substituent(s). In a preferred embodiment, R²Selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, wherein said alkyl is optionally substituted by one or more groups selected from halogen, -NO₂、-CN、C_1-6Alkyl, -NR^a2R^b2Is substituted with the substituent(s). In a more preferred embodiment, R²Selected from H, halogen, C_1-6Alkyl, -OH, wherein the alkyl is optionally substituted by one or more groups selected from halogen, C_1-6Alkyl and-NR^a2R^b2Is substituted with a group (b). In a further embodiment, R²Selected from H, C_1-4Alkyl, -OH, wherein the alkyl is optionally substituted by one or more groups selected from-NR^a2R^b2Is substituted with a group (b). In a particular embodiment, R²Selected from H, C_1-4Alkyl, -OH, wherein the alkyl is-CH₂[CH(CH₃)₂]And optionally substituted by one or more groups selected from-NR^a2R^b2Is substituted with a group (b). In another embodiment, R²Is represented by-NR^a1R^b1A substituted alkyl group. In a particular embodiment, R²Is composed of

In one embodiment, R^X1And R^X2Each occurrence independently selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OR⁷、-SR⁷、-NR⁷R⁸、-C(＝O)OR⁷、-C(＝O)NR⁷R⁸、-OC(＝O)R⁷、-NC(＝O)R⁷R⁸、-C(＝O)R⁷、-S(＝O)₂OR⁷、-S(＝O)₂R⁷、-S(＝O)₂NR⁷R⁸、-OS(＝O)₂R⁷、-NS(＝O)₂R⁷R⁸、-S(＝O)R⁷Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -SH, -S (C)_1-6Alkyl), -S (C)_3-6Cycloalkyl), -S (C)_1-4alkylene-C_3-6Cycloalkyl), -S (three-to seven-membered heterocyclyl), -S (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂And O is substituted with a substituent. In a preferred embodiment, R^X1And R^X2Each occurrence independently selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OR⁷、-SR⁷、-NR⁷R⁸、-OC(＝O)R⁷、-NC(＝O)R⁷R⁸、-OS(＝O)₂R⁷、-NS(＝O)₂R⁷R⁸Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4Alkylene oxideradical-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂Is substituted with the substituent(s). In a preferred embodiment, R^X1And R^X2Independently at each occurrence, is selected from halogen, C_1-6Alkyl, -OR⁷、-NR⁷R⁸More preferably each independently selected from halogen, -OR⁷Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s). In a more preferred embodiment, R^X1And R^X2Each occurrence is independently selected from F, Cl, Br, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Optionally substituted C_1-6Alkyl, wherein said optionally substituted C_1-6Alkyl is optionally substituted by one or more groups selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s). In a particular embodiment, R^X1And R^X2Each occurrence is independently selected from F, Cl, Br, methyl, -OH, dimethylaminomethyl.

R⁷、R⁸Each occurrence is independently selected from H, C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, wherein said alkyl, alkenyl, alkynyl is optionally substituted with one or more substituents selected from the group consisting of halogen, and halogenNO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl) groups. In a preferred embodiment, R⁷、R⁸Each occurrence is independently selected from H, C_1-6Alkyl, wherein said alkyl is optionally substituted by one or more groups selected from halogen, C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl) groups. In a particular embodiment, R⁷、R⁸Each occurrence is independently selected from H, C_1-6Alkyl, wherein the alkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s). In another particular embodiment, R⁷、R⁸Each occurrence is independently selected from H, C_1-6Alkyl, wherein the alkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s).

In one embodiment, R^a1、R^b1、R^c1、R^a2、R^b2、R^c2Each occurrence is independently selected from H, C_1-6Alkyl radical, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, -OR^Y1、-SR^Y1、-NR^Y1R^Y2、-C(＝O)OR^Y1、-C(＝O)NR^Y1R^Y2、-C(＝O)R^Y1Wherein said alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl OR heteroaryl is optionally substituted with one OR more substituents selected from halogen, -OR^Y3、-SR^Y3、-NR^Y3R^Y4、-C(＝O)R^Y3、-C(＝O)OR^Y3and-C (═ O) NR^Y3R^Y4Is substituted with the substituent(s). In a preferred embodiment, R^a1、R^b1、R^c1、R^a2、R^b2、R^c2Each occurrence is independently selected from H, C_1-6Alkyl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, -OR^Y1、-SR^Y1、-NR^Y1R^Y2、-C(＝O)OR^Y1、-C(＝O)NR^Y1R^Y2、-C(＝O)R^Y1Wherein said alkyl, aryl OR heteroaryl is optionally substituted by one OR more groups selected from halogen, -OR^Y3and-NR^Y3R^Y4Is substituted with the substituent(s). In another preferred embodiment, R^a1、R^b1、R^c1、R^a2、R^b2、R^c2Each occurrence is independently selected from H, C_1-6Alkyl, -OR^Y1、-NR^Y1R^Y2、-C(＝O)OR^Y1、-C(＝O)NR^Y1R^Y2、-C(＝O)R^Y1Wherein said alkyl is optionally substituted by one OR more groups selected from halogen, -OR^Y3and-NR^Y3R^Y4Is substituted with a group (b). In a more preferred embodiment, R^a1、R^b1、R^c1、R^a2、R^b2、R^c2Each occurrence is independently selected from H, C_1-6Alkyl, -OR^Y1、-NR^Y1R^Y2、-C(＝O)R^Y1Wherein said alkyl is optionally substituted by one OR more groups selected from halogen, -OR^Y3and-NR^Y3R^Y4Is substituted with a group (b). In a further embodiment, R^a1、R^b1、R^c1、R^a2、R^b2、R^c2Each occurrence is independently selected from H, C_1-6Alkyl, -OR^Y1、-NR^Y1R^Y2、-C(＝O)R^Y1Wherein said alkyl is optionally substituted by one or more substituents selected from halogen, -NR^Y3R^Y4Is substituted with a group (b). In a particular embodiment, R^a1、R^b1、R^c1、R^a2、R^b2、R^c2Each occurrence is independently selected from H, C_1-3Alkyl, -OH, p-methylbenzoyl; wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NH₂Is substituted with a group (b).

In another embodiment, R^Y1、R^Y2、R^Y3、R^Y4Each occurrence is independently selected from H, C_1-8Alkyl radical, C_3-10Cycloalkyl radical, C_3-10Cycloalkyl radical-C_1-4Alkyl, three-to ten-membered heterocyclyl-C_1-4Alkyl radical, C_6-10Aryl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, wherein the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-SH、-NH₂And C_1-6Alkyl substituents. In a preferred embodiment, R^Y1、R^Y2、R^Y3、R^Y4Each occurrence is independently selected from H, C_1-6Alkyl radical, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl, phenyl-C_1-4Alkyl, five-to six-membered heteroaryl-C_1-4Alkyl, wherein the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-SH、-NH₂And C_1-6Alkyl substituents. In a more preferred embodiment, R^Y1、R^Y2、R^Y3、R^Y4Each occurrence is independently selected from H, C_1-6Alkyl, phenyl-C_1-4Alkyl, five-to six-membered heteroaryl-C_1-4Alkyl, wherein the alkyl or phenyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-SH、-NH₂And C_1-6Alkyl substituents. In a more preferred embodiment, R^Y1、R^Y2、R^Y3、R^Y4Each occurrence is independently selected from H, C_1-6Alkyl, phenyl-C_1-4Alkyl, wherein the alkyl or phenyl is optionally substituted by one or more groups selected from halogen, C_1-6Alkyl groups. In a particular embodiment, R^Y1、R^Y2、R^Y3、R^Y4Each occurrence is independently selected from H and p-methylphenyl.

In one embodiment, R³、R⁶Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), wherein the alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl) substituents. In a preferred embodiment, R³、R⁶Each independently selected from H, halogen, -OH, more preferably H or-OH.

In one embodiment, R⁴、R⁵Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -NH (C)_1-4alkylene-C_3-6Cycloalkyl), -NH (three-to seven-membered heterocyclyl), -NH (C)_1-4Alkylene-three-to seven-membered heterocyclyl), -COOH, -C (═ O) O (C)_1-6Alkyl), -C (═ O) NH₂、-C(＝O)NH(C_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl), O, or a substituent of O. In another embodiment, R⁴、R⁵Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-COOH、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH₂、-C(＝O)NH(C_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl), O, or a substituent of O. In yet another embodiment, R⁴、R⁵Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), wherein the alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH₂、-C(＝O)NH(C_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl), O, or a substituent of O. In another embodiment, R⁴、R⁵Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH₂、-C(＝O)NH(C_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl), O, or a substituent of O.

The structures described by the following formula (VII) fall within the scope of the structures described by the formulae (I), (III) and (VI). In another aspect of the present invention, there is provided a use of a compound of formula (VII), or a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite or prodrug thereof, for the preparation of a medicament for the prevention or treatment of a polyQ-related neurodegenerative disease

Wherein:

x is O, NH or S;

Y、R³、R⁴、R⁵、R⁶as defined in formula (VI);

c ring is

Wherein R is¹⁴、R¹⁵、R¹⁶、R¹⁷、R¹⁸Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, -OR⁷、-SR⁷、-NR⁷R⁸、-C(＝O)OR⁷、-C(＝O)NR⁷R⁸、-OC(＝O)R⁷、-NC(＝O)R⁷R⁸、-C(＝O)R⁷、-S(＝O)₂OR⁷、-S(＝O)₂R⁷、-S(＝O)₂NR⁷R⁸、-OS(＝O)₂R⁷、-NS(＝O)₂R⁷R⁸、-S(＝O)R⁷(ii) a Wherein R is⁷、R⁸As defined in formula (VI);

R²selected from H, halogen, C_1-6Alkyl radical, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl, wherein the alkyl, cycloalkyl or heterocyclyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OR^a2、-SR^a2、-NR^a2R^b2、-C(＝O)OR^a2、-C(＝O)NR^a2R^b2、-C(＝O)R^a2、-S(＝O)₂OR^a2、-S(＝O)₂R^a2、-S(＝O)₂NR^a2R^b2and-S (═ O) R^a2Substituted with the substituent(s); wherein R is^a2、R^b2、R^c2As defined in formula (VI);

with the proviso that the compound of formula (VII) does not include the following structure:

wherein R is⁴Is selected from-O (C)_1-6Alkyl), -O (C)_1-4alkylene-C_6-10Aryl).

In one embodiment, Y is O. When Y is O, formula (VII) is

In one embodiment, Y is O and X is N, formula (VII) is

In one embodiment, Y is O and X is S, formula (VII) is

In one embodiment, R²Selected from H, halogen, C_1-6Alkyl, wherein the alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OR^a2、-SR^a2、-NR^a2R^b2、-C(＝O)OR^a2、-C(＝O)NR^a2R^b2、-C(＝O)R^a2、-S(＝O)₂OR^a2、-S(＝O)₂R^a2、-S(＝O)₂NR^a2R^b2and-S (═ O) R^a2Is substituted with the substituent(s). In one embodiment, R²Is selected from-OR^a1、-NR^a1R^b1、-C(＝O)OR^a1、-C(＝O)NR^a1R^b1. In another embodiment, R²Selected from H, halogen, C_1-6Alkyl, wherein the alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-NH₂And a substituent of-COOH.

In one embodiment, R^a2、R^b2、R^c2Each occurrence is independently selected from H, C_1-6Alkyl radical, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl, wherein the alkyl, alkenyl, alkynyl, cycloalkyl or heterocyclyl is optionally substituted with one or more substituents selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-COOH、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s). In a preferred embodiment, R^a2、R^b2、R^c2Each occurrence is independently selected from H, C_1-6Alkyl, wherein the alkyl is optionally substituted with one or more substituents selected from halogen, -OH, -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-COOH、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl radicals),-C(＝O)N(C_1-6Alkyl radical)₂Is substituted with a group (b).

In one embodiment, R³Selected from H, halogen, C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Wherein said alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, C_1-3Alkyl, -OH, -O (C)_1-3Alkyl), -NH₂、-NH(C_1-3Alkyl), -N (C)_1-3Alkyl radical)₂Wherein said alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-2Alkyl), -NH₂、-NH(C_1-2Alkyl), -N (C)_1-2Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, methyl, -OH, -OCH₃、-NH₂、-NHCH₃、-N(CH₃)₂Wherein said methyl is optionally substituted by a group selected from halogen, -OH, -O (C)_1-2Alkyl), -NH₂、-NH(C_1-2Alkyl), -N (C)_1-2Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, methyl, -OH, -OCH₃、-NH₂、-NHCH₃、-N(CH₃)₂Wherein said methyl is optionally substituted by a substituent selected from the group consisting of halogen, -OH, -OCH₃、-NH₂、-NHCH₃、-N(CH₃)₂Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, methyl, -OH, -NH₂、-N(CH₃)₂. In yet another embodiment, R³Selected from H, halogen, C_1-4Alkyl, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl), wherein the alkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl) groups. In one embodiment, R³Selected from H, halogen, methyl, -OH, -OCH₃、-NH₂、-NHCH₃Wherein said methyl is optionally substituted by a group selected from halogen, -OH, -O (C)_1-2Alkyl), -NH₂、-NH(C_1-2Alkyl) groups. In one embodiment, R³Selected from H, halogen, methyl, -OH, -OCH₃、-NH₂、-NHCH₃Wherein said methyl is optionally substituted by a substituent selected from the group consisting of halogen, -OH, -OCH₃、-NH₂、-NHCH₃Is substituted with the substituent(s). In another embodiment, R³Selected from H, halogen, C_1-4Alkyl, -OH, -O (C)_1-4Alkyl), -NH₂Wherein said alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-4Alkyl), -NH₂Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, methyl, -OH, -OCH₃、-NH₂Wherein said methyl is optionally substituted by a group selected from halogen, -OH, -O (C)_1-2Alkyl), -NH₂Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, methyl, -OH, -OCH₃、-NH₂Wherein said methyl is optionally substituted by a substituent selected from the group consisting of halogen, -OH, -OCH₃、-NH₂Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, methyl, -OH, -NH₂. In another embodiment, R³Selected from H, halogen, C_1-4Alkyl, -OH, -O (C)_1-4Alkyl), wherein the alkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -O (C)_1-4Alkyl) groups. In one embodiment, R³Selected from H, halogen, methyl, -OH, -OCH₃Wherein said methyl is optionally substituted by a group selected from halogen, -OH, -O (C)_1-2Alkyl) groups. In one embodiment, R³Selected from H, halogen, methyl, -OH, -OCH₃Wherein said methyl is optionally substituted by a substituent selected from the group consisting of halogen, -OH, -OCH₃Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, methyl, -OH. In yet another embodiment, R³Selected from H, halogen, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl), -N (C)_1-4Alkyl radical)₂Substituted C_1-4Alkyl, wherein said substituted C_1-4Alkyl is substituted by one or more groups selected from halogen, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl), -N (C)_1-4Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, -OH, -OCH₃、-NH₂、-NHCH₃、-N(CH₃)₂Substituted methyl, wherein the substituted methyl is selected from the group consisting of halogen, -OH, -O (C)_1-2Alkyl), -NH (C)_1-2Alkyl), -N (C)_1-2Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, -OH, -OCH₃、-NH₂、-NHCH₃、-N(CH₃)₂Substituted methyl, wherein the substituted methyl is selected from the group consisting of halogen, -OH, -OCH₃、-NHCH₃、-N(CH₃)₂Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, -OH, -NH₂Methyl, wherein the methyl is represented by-N (CH)₃)₂And (4) substitution. In yet another embodiment, R³Selected from H, halogen, -OH, -O (C)_1-4Alkyl), substituted C_1-4Alkyl, wherein said substituted C_1-4Alkyl is substituted by one or more groups selected from halogen, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl), -N (C)_1-4Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, -OH, -OCH₃Substituted methyl, wherein the substituted methyl is selected from the group consisting of halogen, -OH, -O (C)_1-2Alkyl), -NH (C)_1-2Alkyl), -N (C)_1-2Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, -OH, -OCH₃Substituted methyl, wherein the substituted methyl is selected from the group consisting of halogen, -OH, -OCH₃、-NHCH₃、-N(CH₃)₂Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, -OH, methyl, wherein the methylradical-N (CH)₃)₂And (4) substitution. In another embodiment, R³Selected from H, halogen, -OH, -O (C)_1-4Alkyl), substituted C_1-4Alkyl, wherein said substituted C_1-4Alkyl is substituted by one or more groups selected from halogen, -NH₂、-NH(C_1-4Alkyl), -N (C)_1-4Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, -OH, C_1-2Alkyl, wherein the alkyl is substituted with one or more substituents selected from the group consisting of halogen, -NH (C)_1-2Alkyl), -N (C)_1-2Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, -OH, methyl, wherein said methyl is selected from halogen, -NH (C)_1-2Alkyl), -N (C)_1-2Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, -OH, methyl, wherein said methyl is selected from halogen, -NHCH₃、-N(CH₃)₂Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, -OH, methyl, wherein said methyl is-N (CH)₃)₂And (4) substitution. In yet another embodiment, R³Selected from H, halogen, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl), substituted C_1-4Alkyl, wherein said substituted C_1-4Alkyl is substituted by one or more groups selected from halogen, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl) groups. In one embodiment, R³Selected from H, halogen, -OH, -OCH₃、-NH₂、-NHCH₃Substituted methyl, wherein the substituted methyl is selected from the group consisting of halogen, -OH, -O (C)_1-2Alkyl), -NH (C)_1-2Alkyl) groups. In one embodiment, R³Selected from H, halogen, -OH, -OCH₃、-NH₂、-NHCH₃Substituted methyl, wherein the substituted methyl is selected from the group consisting of halogen, -OH, -OCH₃、-NHCH₃Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, -OH, -NH₂Methyl, wherein the methyl is represented by-N (CH)₃)₂And (4) substitution. In yet another embodiment, R³Selected from H, halogen, -OH, -O (C)_1-4Alkyl), substituted C_1-4Alkyl, wherein said substituted C_1-4Alkyl is substituted by one or more groups selected from halogen, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl) groups. In one embodiment, R³Selected from H, halogen, -OH, -OCH₃Substituted methyl, wherein the substituted methyl is selected from the group consisting of halogen, -OH, -O (C)_1-2Alkyl), -NH (C)_1-2Alkyl) groups. In one embodiment, R³Selected from H, halogen, -OH, -OCH₃Substituted methyl, wherein the substituted methyl is selected from the group consisting of halogen, -OH, -OCH₃、-NHCH₃Is substituted with the substituent(s). In one embodiment, R³Selected from H, halogen, -OH, methyl, wherein said methyl is-N (CH)₃)₂And (4) substitution. In another embodiment, R³Selected from H, halogen, -OH, -O (C)_1-4Alkyl), substituted C_1-4Alkyl, wherein said substituted C_1-4Alkyl is substituted by one or more groups selected from halogen, -NH₂、-NH(C_1-4Alkyl) groups. In one embodiment, R³Selected from H, halogen, -OH, C_1-2Alkyl, wherein the alkyl is substituted with one or more substituents selected from the group consisting of halogen, -NH (C)_1-2Alkyl) groups. In one embodiment, R³Selected from H, halogen, -OH, methyl, wherein said methyl is selected from halogen, -NH (C)_1-2Alkyl) groups. In one embodiment, R³Selected from H, halogen, -OH, methyl, wherein said methyl is selected from halogen, -NHCH₃Is substituted with the substituent(s). In one embodiment, R³Selected from H, F, Cl, methyl, -OH, -NH₂. In another embodiment, R³Selected from H, F, methyl, -OH, -NH₂. In one embodiment, R³Is H. In one embodiment, R³is-OH. In one embodiment, R³Is CH₃。

In one embodiment, R⁴Is selected fromH. Halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Wherein said alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R⁴Selected from H, halogen, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl), -N (C)_1-4Alkyl radical)₂Substituted C_1-4Alkyl, wherein said substituted C_1-4Alkyl is substituted by one or more groups selected from halogen, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl), -N (C)_1-4Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R⁴Selected from H, halogen, -OH, -O (C)_1-3Alkyl), -NH₂、-NH(C_1-3Alkyl), -N (C)_1-3Alkyl radical)₂Substituted C_1-3Alkyl, wherein said substituted C_1-3Alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-2Alkyl), -NH₂、-NH(C_1-2Alkyl), -N (C)_1-2Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R⁴Selected from H, halogen, -OH, -NH₂、-NH(C_1-4Alkyl), substituted C_1-4Alkyl, substituted-O (C)_1-4Alkyl), substituted-N (C)_1-4Alkyl radical)₂Wherein said substituted C_1-4Alkyl, substituted-O (C)_1-4Alkyl) and substituted-N (C)_1-4Alkyl radical)₂By one or more radicals selected from halogen, -OH, -O (C)_1-2Alkyl), -NH₂、-NH(C_1-4Alkyl) groups. In one embodiment, R⁴Selected from H, halogen, -OH, -O (C)_1-2Alkyl), -NH₂、-NH(C_1-2Alkyl), substituted C_1-2Alkyl, substituted-O (C)_1-2Alkyl) wherein said substituted C_1-2Alkyl and substituted-O (C)_1-2Alkyl) with one or more substituents selected from halogen, -OH、-O(C_1-2Alkyl), -NH₂、-NH(C_1-2Alkyl) groups. In one embodiment, R⁴Selected from H, halogen, -OH, -OCH₃、-NH₂、-NHCH₃Substituted C_1-2Alkyl, wherein said substituted C_1-2Alkyl is substituted by one or more groups selected from halogen, -OH, -OCH₃、-NH₂、-NHCH₃Is substituted with the substituent(s). In one embodiment, R⁴Selected from H, halogen, -OH, -NH₂、-NH(C_1-4Alkyl), substituted C_1-4Alkyl, wherein said substituted C_1-4Alkyl is substituted by one or more groups selected from halogen, -OH, -OCH₃、-NH₂、-NH(C_1-4Alkyl) groups. In one embodiment, R⁴Selected from H, halogen, -OH, -NH₂、-NH(C_1-2Alkyl), -N (C)_1-2Alkyl radical)₂Substituted C_1-2Alkyl, wherein said substituted C_1-2Alkyl is substituted by one or more groups selected from halogen, -OH, -O (C)_1-2Alkyl), -NH₂、-NH(C_1-2Alkyl) groups. In one embodiment, R⁴Selected from H, halogen, -OH, -NH₂、-NHCH₃Substituted C_1-2Alkyl, wherein said substituted C_1-2Alkyl is substituted by one or more groups selected from halogen, -OH, -OCH₃、-NH₂、-NHCH₃Is substituted with the substituent(s). In one embodiment, R⁴Selected from H, halogen, C_1-4Alkyl, -OH, -O (C)_1-4Alkyl), wherein the alkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl), -N (C)_1-4Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R⁴Selected from H, halogen, C_1-2Alkyl, -OH, -OCH₃Wherein said alkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -OCH₃、-NH₂、-NHCH₃、-N(CH₃)₂Is substituted with the substituent(s). In one embodiment, R⁴Selected from H, halogen, C_1-4Alkyl, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl), -N (C)_1-4Alkyl radical)₂Wherein said alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-4Alkyl) groups. In one embodiment, R⁴Selected from H, halogen, C_1-2Alkyl, -OH, -OCH₃、-NH₂、-NHCH₃、-N(CH₃)₂Wherein said alkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -OCH₃Is substituted with the substituent(s). In one embodiment, R⁴Selected from H, halogen, C_1-4Alkyl, -OH, -O (C)_1-4Alkyl), wherein the alkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -O (C)_1-4Alkyl) groups. In one embodiment, R⁴Selected from H, halogen, C_1-2Alkyl, -OH, -OCH₃Wherein said alkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -OCH₃Is substituted with the substituent(s). In one embodiment, R⁴Selected from H, -OH, -OCH₃. In one embodiment, R⁴Is H. In one embodiment, R⁴is-OH.

In one embodiment, R⁵Selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl), wherein the alkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl) groups. In one embodiment, R⁵Selected from H, halogen,C_1-4Alkyl, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl), -N (C)_1-4Alkyl radical)₂Wherein said alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl), -N (C)_1-4Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R⁵Selected from H, halogen, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl), -N (C)_1-4Alkyl radical)₂、-C(＝O)O(C_1-4Alkyl), -C (═ O) NH₂、-C(＝O)NH(C_1-4Alkyl), -C (═ O) N (C)_1-4Alkyl radical)₂、-OC(＝O)(C_1-4Alkyl), -NC (═ O) (C)_1-4Alkyl radical)₂、-C(＝O)(C_1-4Alkyl), substituted C_1-4Alkyl, wherein said substituted C_1-4Alkyl is substituted by one or more groups selected from halogen, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl), -N (C)_1-4Alkyl radical)₂、-C(＝O)O(C_1-4Alkyl), -C (═ O) NH₂、-C(＝O)NH(C_1-4Alkyl), -C (═ O) N (C)_1-4Alkyl radical)₂、-OC(＝O)(C_1-4Alkyl), -NC (═ O) (C)_1-4Alkyl radical)₂、-C(＝O)(C_1-4Alkyl) groups. In one embodiment, R⁵Selected from H, halogen, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl), -N (C)_1-4Alkyl radical)₂Substituted C_1-4Alkyl, wherein said substituted C_1-4Alkyl is substituted by one or more groups selected from halogen, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl), -N (C)_1-4Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R⁵Selected from H, halogen, -O (C)_1-4Alkyl), -NH (C)_1-4Alkyl), -N (C)_1-4Alkyl radical)₂Substituted C_1-2Alkyl, wherein said substituted C_1-2Alkyl is substituted by one or more groups selected from halogen, -O (C)_1-4Alkyl), -NH (C)_1-4Alkyl), -N (C)_1-4Alkyl radical)₂Is substituted with the substituent(s). In one embodiment, R⁵Selected from H, halogen, -O (C)_1-4Alkyl), -NH (C)_1-4Alkyl), -N (C)_1-4Alkyl radical)₂Substituted C_1-2Alkyl, wherein said substituted C_1-2Alkyl is substituted by one or more groups selected from halogen, -OCH₃、-NHCH₃、-N(CH₃)₂Is substituted with the substituent(s). In one embodiment, R⁵Selected from H, halogen, -O (C)_1-2Alkyl), -NH (C)_1-2Alkyl), -N (C)_1-2Alkyl radical)₂Substituted C_1-2Alkyl, wherein said substituted C_1-2Alkyl is substituted by one or more groups selected from halogen, -OCH₃、-NHCH₃、-N(CH₃)₂Is substituted with the substituent(s).

In one embodiment, R⁶Selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Wherein said alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s). In another embodiment, R⁶Selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -OH, -NH₂Is substituted with the substituent(s). In one embodiment, R⁶Selected from H, halogen, -NO₂、-CN、C_1-2Alkyl, -OH, -O (C)_1-2Alkyl), -NH₂、-NH(C_1-2Alkyl), -N (C)_1-2Alkyl radical)₂Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -OH, -NH₂Is substituted with the substituent(s). In yet another embodiment, R⁶Selected from H, halogen, -NO₂、-CN、C_1-4Alkyl, -OH, -O (C)_1-4Alkyl), -NH₂、-NH(C_1-4Alkyl), -N- (O-X-O) - (Y-OC_1-4Alkyl radical)₂Wherein said alkyl is optionally substituted with one or more halogens. In another embodiment, R⁶Selected from H, halogen, -NO₂、-CN、C_1-2Alkyl, -OH, -O (C)_1-2Alkyl), -NH₂、-NH(C_1-4Alkyl), -N (C)_1-4Alkyl radical)₂. In one embodiment, R⁶Selected from H, F, Cl, Br, C_1-6Alkyl, -OH, -NH₂Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -OH, -NH₂Is substituted with the substituent(s). In another embodiment, R⁶Selected from H, F, Cl, Br, methyl, -OH, -NH₂. In a particular embodiment, R⁶Is H or-OH, especially H.

In one embodiment, R³Is methyl, and R⁴Selected from-OH, -NH₂、-NH(C_1-3Alkyl), -N (C)_1-3Alkyl radical)₂C substituted by-COOH_1-3Alkyl, wherein said substituted C_1-3Alkyl is substituted by one or more groups selected from-OH, -NH₂、-NH(C_1-2Alkyl), -N (C)_1-2Alkyl radical)₂And a substituent of-COOH. In one embodiment, R³Is methyl, and R⁴Is selected from-NH₂、-NH(C_1-3Alkyl), -N (C)_1-3Alkyl radical)₂C substituted by-COOH_1-3Alkyl, wherein said substituted C_1-3Alkyl is substituted by one or more groups selected from-OH, -NH₂、-NH(C_1-2Alkyl), -N (C)_1-2Alkyl radical)₂And a substituent of-COOH.

In another embodiment, R³Is methyl, and R⁵Selected from-OH, -NH₂、-NH(C_1-3Alkyl), -N (C)_1-3Alkyl radical)₂C substituted by-COOH_1-3Alkyl, wherein said substituted C_1-3Alkyl is substituted by one or more groups selected from-OH, -NH₂、-NH(C_1-2Alkyl), -N (C)_1-2Alkyl radical)₂And a substituent of-COOH. In another embodiment, R³Is methyl, and R⁵Is selected from-NH₂、-NH(C_1-3Alkyl), -N (C)_1-3Alkyl radical)₂C substituted by-COOH_1-3Alkyl, wherein said substituted C_1-3Alkyl is substituted by one or more groups selected from-OH, -NH₂、-NH(C_1-2Alkyl), -N (C)_1-2Alkyl radical)₂And a substituent of-COOH.

In one embodiment, R⁴And R⁵Together with the C atom to which they are attached form a five-to seven-membered heterocyclic ring containing 1 or 2 heteroatoms each independently selected from N, O and S, including at least one N atom, and optionally substituted with one or more substituents selected from halogen and C_1-6Alkyl, preferably said heterocycle is optionally substituted with one or more methyl or ethyl groups. In one embodiment, R⁴And R⁵Together with the C atom to which they are attached form a five-to seven-membered heterocyclic ring containing 1N atom and having the formula (VII)

In which R is¹²Selected from H, methyl and ethyl; r¹³Selected from halogen, C_1-6An alkyl group; q is 0,1, 2 or 3. In one embodiment, X and Y are O, formula (VII) is

In which R is¹²Selected from H, methyl and ethyl; r¹³Selected from halogen, C_1-6An alkyl group; q is 0,1, 2 or 3.

In one embodiment, R¹⁴、R¹⁵、R¹⁶、R¹⁷、R¹⁸Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclic group), -O (C ═ O) (C)_1-6Alkyl), -O (C ═ O) (C)_3-6Cycloalkyl), -O (C ═ O) (C)_1-4alkylene-C_3-6Cycloalkyl), -O (C ═ O), (C) and (C ═ O)Three-to seven-membered heterocyclic group), -O (C ═ O) (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -SH, -S (C)_1-6Alkyl), -S (C)_3-6Cycloalkyl), -S (C)_1-4alkylene-C_3-6Cycloalkyl), -S (three-to seven-membered heterocyclyl), -S (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂、-NH(C＝O)(C_1-6Alkyl), -NH (C ═ O) (C)_3-6Cycloalkyl), -NH (C ═ O) (C)_1-4alkylene-C_3-6Cycloalkyl), -NH (C ═ O) (three-to seven-membered heterocyclyl), -C (═ O) (C)_1-6Alkyl), -C (═ O) O (C)_1-6Alkyl), -C (═ O) O (C)_3-6Cycloalkyl), -C (═ O) O (C)_1-4alkylene-C_3-6Cycloalkyl), -C (═ O) O (three-to seven-membered heterocyclyl), -C (═ O) O (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -C (═ O) NH₂、-C(＝O)NH(C_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-C(＝O)NH(C_1-4alkylene-C_3-6Cycloalkyl), -C (═ O) N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂-C (═ O) NH (three-to seven-membered heterocyclic group), -C (═ O) N (three-to seven-membered heterocyclic group)₂、-C(＝O)NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -C (═ O) N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂Wherein said alkyl, alkylene, alkenyl, alkynyl, cycloalkyl or heterocyclyl is optionally substituted with one or more substituents selected from H, halogen, nitro, cyano, -OH, -SH, -NH₂O and-COOH. In one embodiment, R¹⁴、R¹⁵、R¹⁶、R¹⁷、R¹⁸Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OR⁷、-NR⁷R⁸、-C(＝O)OR⁷、-C(＝O)NR⁷R⁸、-OC(＝O)R⁷、-NC(＝O)R⁷R⁸、-C(＝O)R⁷. In one embodiment, R¹⁴、R¹⁵、R¹⁶、R¹⁷、R¹⁸Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclic group), -O (C ═ O) (C)_1-6Alkyl), -O (C ═ O) (C)_3-6Cycloalkyl), -O (C ═ O) (C)_1-4alkylene-C_3-6Cycloalkyl), -O (C ═ O) (three-to seven-membered heterocyclic group), -O (C ═ O) (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂、-NH(C＝O)(C_1-6Alkyl), -NH (C ═ O) (C)_3-6Cycloalkyl), -NH (C ═ O) (C)_1-4alkylene-C_3-6Cycloalkyl), -NH (C ═ O) (three-to seven-membered heterocyclyl). In one embodiment, R¹⁴、R¹⁵、R¹⁷、R¹⁸Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclic group), -O (C ═ O) (C)_1-6Alkyl), -O (C ═ O) (C)_3-6Cycloalkyl), -O (C ═ O) (C)_1-4alkylene-C_3-6Cyclic hydrocarbonsAlkyl), -O (C ═ O) (three-to seven-membered heterocyclyl), -O (C ═ O) (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂、-NH(C＝O)(C_1-6Alkyl), -NH (C ═ O) (C)_3-6Cycloalkyl), -NH (C ═ O) (C)_1-4alkylene-C_3-6Cycloalkyl), -NH (C ═ O) (three-to seven-membered heterocyclyl). In one embodiment, R¹⁴、R¹⁵、R¹⁶、R¹⁷、R¹⁸Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclic group), -O (C ═ O) (C)_1-6Alkyl), -O (C ═ O) (C)_3-6Cycloalkyl), -O (C ═ O) (C)_1-4alkylene-C_3-6Cycloalkyl), -O (C ═ O) (three-to seven-membered heterocyclic group), -O (C ═ O) (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl). In one embodiment, R¹⁴、R¹⁵、R¹⁶、R¹⁷、R¹⁸Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclic group), -O (C ═ O) (C)_1-6Alkyl), -O (C ═ O) (C)_3-6Cycloalkyl), -O (C ═ O) (C)_1-4alkylene-C_3-6Cycloalkyl), -O (C ═ O) (three-to seven-membered heterocyclic group), -O (C ═ O) (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl).In one embodiment, R¹⁴、R¹⁵、R¹⁶、R¹⁷、R¹⁸Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂、-NH(C＝O)(C_1-6Alkyl), -NH (C ═ O) (C)_3-6Cycloalkyl), -NH (C ═ O) (C)_1-4alkylene-C_3-6Cycloalkyl), -NH (C ═ O) (three-to seven-membered heterocyclyl). In one embodiment, R¹⁴、R¹⁵、R¹⁶、R¹⁷、R¹⁸Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂、-NH(C＝O)(C_1-6Alkyl), -NH (C ═ O) (C)_3-6Cycloalkyl), -NH (C ═ O) (C)_1-4alkylene-C_3-6Cycloalkyl), -NH (C ═ O) (three-to seven-membered heterocyclyl). In one embodiment, R¹⁴、R¹⁵、R¹⁶、R¹⁷、R¹⁸Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C＝O)(C_1-6Alkyl groups). In an implementation methodIn the scheme, R¹⁴、R¹⁵、R¹⁷、R¹⁸Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C＝O)(C_1-6Alkyl groups). In one embodiment, R¹⁶Is H. In one embodiment, R¹⁶is-OCH₃。

The structures described by the following formula (VIII) fall within the scope of the structures described by formula (I), formula (III), formula (VI) and formula (VII). In another aspect of the present invention, there is provided a use of a compound of formula (VIII), or a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite or prodrug thereof, for the preparation of a medicament for the prevention or treatment of a polyQ-related neurodegenerative disease

Wherein:

c ring, Y, R²、R³、R⁴、R⁵、R⁶As defined in formula (VII);

in one embodiment, Y is O. When Y is O, formula (VIII) is

In a particular embodiment, the compound of formula (VIII) is selected from:

in another particular embodiment, the compound of formula (VIII) is selected from:

the structures described by the following formula (IX) fall within the scope of the structures described by the formulae (I), (III) and (VI). In another aspect of the present invention, there is provided a use of a compound of formula (IX), or a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite or prodrug thereof, for the preparation of a medicament for the prevention or treatment of a polyQ-related neurodegenerative disease

Wherein:

R¹⁹each occurrence independently selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OR⁷、-SR⁷、-NR⁷R⁸、-C(＝O)OR⁷、-C(＝O)NR⁷R⁸、-OC(＝O)R⁷、-NC(＝O)R⁷R⁸、-C(＝O)R⁷、-S(＝O)₂OR⁷、-S(＝O)₂R⁷、-S(＝O)₂NR⁷R⁸、-OS(＝O)₂R⁷、-NS(＝O)₂R⁷R⁸、-S(＝O)R⁷Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂-COOH;

m is 0,1, 2,3, 4 or 5;

R²、R³、R⁴、R⁵、R⁶as defined in formula (VI).

In one embodiment, R²Selected from H, halogen, -NO₂、-CN、C_1-6Alkyl radical, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl, -OR^a1、-SR^a1、-NR^a1R^b1、-C(＝O)OR^a1、-C(＝O)NR^a1R^b1、-C(＝O)R^a1、-S(＝O)₂OR^a1、-S(＝O)₂R^a1、-S(＝O)₂NR^a1R^b1、-S(＝O)R^a1Wherein said alkyl, cycloalkyl or heterocyclyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OR^a2、-SR^a2、-NR^a2R^b2、-C(＝O)OR^a2、-C(＝O)NR^a2R^b2、-C(＝O)R^a2Is substituted with the substituent(s). In a preferred embodiment, R²Selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, wherein said alkyl is optionally substituted by one or more groups selected from halogen, -NO₂、-CN、C_1-6Alkyl, -NR^a2R^b2Is substituted with the substituent(s). In a more preferred embodiment, R²Selected from H, halogen, C_1-6Alkyl, -OH, wherein the alkyl is optionally substituted by one or more groups selected from halogen, C_1-6Alkyl and-NR^a2R^b2Is substituted with a group (b). In a further embodiment, R²Selected from H, C_1-4Alkyl, -OH, wherein the alkyl is optionally substituted by one or more groups selected from-NR^a2R^b2Is substituted with a group (b). In a particular embodiment, R²Selected from H, C_1-4Alkyl, -OH, wherein the alkyl is-CH₂[CH(CH₃)₂]And optionally substituted by one or more groups selected from-NR^a2R^b2Is substituted with a group (b). In another embodiment, R²Is represented by-NR^a1R^b1A substituted alkyl group. R^a1、R^b1、R^c1、R^a2、R^b2、R^c2As defined in formula (VI). In a particular embodiment, R²Is composed of

In one embodiment, R¹⁹Independently at each occurrence, is selected from halogen, C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Wherein said alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s).

In a particular embodiment, the compound of formula (IX) is

The structures described by the following formula (X) fall within the scope of the structures described by the formulae (I) and (III). In another aspect of the present invention, there is provided a use of a compound of formula (X), or a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite or prodrug thereof, for the preparation of a medicament for the prevention or treatment of a polyQ-related neurodegenerative disease

Wherein:

R⁷selected from H, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂(ii) a Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂-COOH;

z is CR²⁰Or N;

R²⁰selected from H, halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂(ii) a Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂-COOH;

R⁹selected from H, halogen, C_1-6Alkyl radical, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl, -OR^a1、-SR^a1、-NR^a1R^b1、-C(＝O)OR^a1、-C(＝O)NR^a1R^b1、-C(＝O)R^a1、-S(＝O)₂OR^a1、-S(＝O)₂R^a1、-S(＝O)₂NR^a1R^b1、-S(＝O)R^a1Wherein said alkyl, cycloalkyl or heterocyclyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OR^a2、-SR^a2、-NR^a2R^b2、-C(＝O)OR^a2、-C(＝O)NR^a2R^b2、-C(＝O)R^a2、-S(＝O)₂OR^a2、-S(＝O)₂R^a2、-S(＝O)₂NR^a2R^b2and-S (═ O) R^a2Substituted with the substituent(s); wherein R is^a1、R^b1、R^a2、R^b2As defined in formula (III);

R¹⁰selected from H, halogen, C_1-6Alkyl radical, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4An alkyl group;

R³selected from H, halogen, C_1-6Alkyl, -OH, -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Wherein said alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Substituted with the substituent(s);

R⁴selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OR⁷、-SR⁷、-NR⁷R⁸；R⁷、R⁸Each occurrence is independently selected from H, C_1-6Alkyl, wherein the alkyl is optionally substituted with one or more substituents selected from halogen、-NO₂、-CN、-OH、-O(C_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-COOH、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl) substituted with a substituent; wherein R is⁷、R⁸As defined in formula (III);

R⁵selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl), wherein the alkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl) substituted with a substituent;

R⁶selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -O (benzyl), -SH, -S (C)_1-6Alkyl), -S (benzyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂-NH (benzyl), wherein said alkyl or benzyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s).

In one embodiment, Z is CR²⁰In the formula (X) is

And:

R²⁰selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂(ii) a Wherein said alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Substituted with the substituent(s); or

R²⁰Is hydrogen, and R³Selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂(ii) a Wherein said alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s).

In another embodiment, Z is CR²⁰And:

R²⁰selected from halogen, -OH, -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂(ii) a Or

R²⁰Is hydrogen, and R³Selected from-OH, halogen, -NH₂。

In yet another embodiment, Z is CR²⁰And:

R²⁰selected from F, Cl, -OH, -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂(ii) a Or

R²⁰Is hydrogen, and R³Selected from-OH, F, Cl, -NH₂。

In one embodiment, Z is N and formula (X) is

And R is³Selected from OH, halogen, -NH₂。

In one embodiment, Z is N, and R is³Selected from OH, F, -NH₂。

The structures described below in formula (XI) fall within the scope of the structures described in formulas (I) and (III). In another aspect of the present invention, there is provided a use of a compound of formula (XI), or a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite or prodrug thereof, for the preparation of a medicament for the prevention or treatment of a polyQ-related neurodegenerative disease

Wherein:

R²⁰selected from H, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂(ii) a Wherein said alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂-COOH;

z is CR²⁰Or N;

R²⁰selected from H, halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂(ii) a Wherein said alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂-COOH;

c ring, R²、R³、R⁴、R⁵、R⁶As defined in formula (III).

In one embodiment, Z is CR²⁰Formula (XI) is

And:

R²⁰selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂(ii) a Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Substituted with the substituent(s); or

R²⁰Is hydrogen, and R³Selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂(ii) a Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Is substituted with the substituent(s).

In another embodiment, Z is CR²⁰And:

R²⁰selected from halogen, -OH, -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂(ii) a Or

R²⁰Is hydrogen, and R³Selected from-OH, halogen, -NH₂。

In yet another embodiment, Z is CR²⁰And:

R²⁰selected from F, Cl, -OH, -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂(ii) a Or

R²⁰Is hydrogen, and R³Selected from-OH, F, Cl, -NH₂。

In one embodiment, Z is N and formula (XI) is

And R is³Selected from OH, halogen, -NH₂。

In one embodiment, Z is N, and R is³Selected from OH, F, -NH₂。

In a further aspect, the present invention provides the use of a pharmaceutical composition of a compound of any one of formula (I), formula (II), formula (III '), formula (IV), formula (V), formula (VI), formula (VII), formula (VIII), formula (IX), formula (X) and formula (XI) comprising a compound of any one of formula (I), formula (II), formula (III'), formula (IV), formula (V), formula (VI), formula (VII), formula (VIII), formula (IX), formula (X) and formula (XI), or a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite or prodrug thereof, in the manufacture of a medicament for the prevention or treatment of a polyQ-related neurodegenerative disease.

In another aspect, the present invention provides a compound of any one of formula (I), formula (II), formula (III'), formula (IV), formula (V), formula (VI), formula (VII), formula (VIII), formula (IX), formula (X), and formula (XI), or a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite, or prodrug thereof, or a pharmaceutical composition thereof, for use in preventing or treating a polyQ-related neurodegenerative disease.

In a further aspect, the present invention provides a method for preventing or treating a polyQ-related neurodegenerative disease, the method comprising administering to a subject in need thereof a compound of any one of formula (I), formula (II), formula (III'), formula (IV), formula (V), formula (VI), formula (VII), formula (VIII), formula (IX), formula (X), and formula (XI), or a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite, or prodrug thereof, or a pharmaceutical composition thereof.

The compound of the present invention can be administered to a patient orally or parenterally in the form of conventional formulations such as capsules, microcapsules, tablets, granules, powders, lozenges, pills, suppositories, injections, suspensions, syrups, patches, creams, lotions, ointments, gels, sprays, solutions and emulsions. Suitable formulations can be prepared by a method generally employed using conventional organic or inorganic additives, such as excipients (e.g., sucrose, starch, mannitol, sorbitol, lactose, glucose, cellulose, talc, calcium phosphate, or calcium carbonate), binders (e.g., cellulose, methyl cellulose, hydroxymethyl cellulose, polypropylpyrrolidone, polyvinylpyrrolidone, gelatin, acacia, polyethylene glycol, sucrose, or starch), disintegrants (e.g., starch, carboxymethyl cellulose, hydroxypropyl starch, low-substituted hydroxypropyl cellulose, sodium bicarbonate, calcium phosphate, or calcium citrate), lubricants (e.g., magnesium stearate, light anhydrous silicic acid, talc, or sodium lauryl sulfate), flavoring agents (e.g., citric acid, menthol, glycine, or orange powder), preservatives (e.g., sodium benzoate, sodium bisulfite, methyl or propyl paraben), stabilizers (e.g., citric acid, sodium citrate or acetic acid), suspending agents (e.g., methylcellulose, polyvinylpyrrolidone or aluminum stearate), dispersing agents (e.g., hydroxypropylmethylcellulose), diluents (e.g., water), and waxes (e.g., cocoa butter, white petrolatum or polyethylene glycol). For example, an effective amount of the compound in a pharmaceutical composition can be an amount that achieves the desired effect.

The dosing regimen may be adjusted to provide the best desired response. For example, when administered as an injection, a single bolus, bolus and/or continuous infusion may be administered, and the like. For example, several divided doses may be administered over time, or the dose may be proportionally reduced or increased as indicated by the exigencies of the therapeutic situation. It is noted that dosage values may vary with the type and severity of the condition being alleviated, and may include single or multiple doses. Generally, the dosage of treatment will vary depending on considerations such as, for example: the age, sex and general health of the patient to be treated; the frequency of treatment and the nature of the desired effect; the degree of tissue damage; duration of symptoms; and other variables that can be adjusted by the individual physician. It is further understood that for any particular individual, the specific dosage regimen will be adjusted over time according to the individual need and the professional judgment of the person administering the composition or supervising the administration of the composition. The amount and regimen of administration of the pharmaceutical composition can be readily determined by one of ordinary skill in the clinical art. For example, the composition or compound of the present invention may be administered in divided doses 4 times per day to 1 time per 3 days, and the amount administered may be, for example, 0.01 to 1000 mg/time. The desired dose may be administered in one or more administrations to achieve the desired result. The pharmaceutical compositions according to the invention may also be provided in unit dosage form.

In one embodiment, the invention provides a capsule containing a compound of the invention without an additional carrier.

The pharmaceutical compositions of the present invention may be in the form of tablets, chewable tablets, capsules, solutions, parenteral solutions, lozenges, suppositories, suspensions and the like. The compositions may be formulated to contain the daily dose or a suitable fraction of the daily dose in a dosage unit which may be a single tablet or capsule or a suitable volume of liquid.

In one embodiment, the solution is prepared from a water soluble salt, such as a hydrochloride salt. In general, all compositions are prepared according to known methods in pharmaceutical chemistry. Capsules may be prepared by mixing the compound with a suitable carrier or diluent and filling the capsules with an appropriate amount of the mixture. Commonly used carriers and diluents include, but are not limited to, inert powdered materials such as various starches, powdered cellulose, especially crystalline and microcrystalline cellulose, sugars such as fructose, mannitol and sucrose, cereal flour and similar edible powders.

Tablets may be prepared by direct compression, wet granulation or dry granulation. The preparation thereof usually contains a diluent, a binder, a lubricant and a disintegrant as well as the compound. Typical diluents include, for example, various types of starch, lactose, mannitol, kaolin, calcium phosphate or sulfate, inorganic salts (such as sodium chloride) and powdered sugar. Powdered cellulose derivatives are also useful. Typical tablet binders are substances such as starch, gelatin and sugars (e.g. lactose, fructose, glucose, etc.). Natural and synthetic gums are also suitable, including acacia, alginate, methylcellulose, polyvinylpyrrolidone, and the like. Polyethylene glycol, ethyl cellulose, and waxes may also serve as binders.

Lubricants may be selected from such slippery solids as talc, magnesium and calcium stearate, stearic acid and hydrogenated vegetable oils. Tablet disintegrants are compounds that swell when wetted to break the tablet and release the compound. They include starches, clays, celluloses, algins and gums. More particularly, it is possible to use, for example, corn and potato starch, methylcellulose, agar, bentonite, wood cellulose, powdered natural sponge, anion exchange resins, alginic acid, guar gum, citrus pulp and carboxymethylcellulose, and sodium lauryl sulfate. The tablets may be coated with sugar as a flavoring and sealing agent, or with a film-forming protecting agent to optimize the dissolution properties of the tablet. The compositions may also be formulated as chewable tablets, for example by incorporating substances such as mannitol into the formulation.

Typical bases may be used when administration as a suppository is desired. Cocoa butter is a traditional suppository base that may be modified by adding waxes to raise its melting point slightly. Water-miscible suppository bases, particularly including polyethylene glycols of various molecular weights, are widely used.

The effect of the compounds can be delayed or prolonged by suitable formulations. For example, slowly dissolving pellets of the compound may be prepared and incorporated into a tablet or capsule or as a slow release implantable device. The technique also involves preparing pellets of several different dissolution rates and filling the capsule with a mixture of pellets. The tablet or capsule may be coated with a film that resists dissolution for a predictable period of time. Even parenteral formulations can be prepared to be long acting by dissolving or suspending the compound in an oily or emulsified vehicle which allows it to be slowly dispersed in serum.

In a preferred embodiment, the polyQ-related neurodegenerative disease is selected from spinocerebellar ataxia 1, spinocerebellar ataxia 2, spinocerebellar ataxia 3, spinocerebellar ataxia 7, spinocerebellar ataxia 12, spinocerebellar ataxia 17, dentate nucleus-substantia nigra-red nucleus-globus-thalamus atrophy, Huntington's disease and spinobulbar muscular atrophy. In a preferred embodiment, the neurodegenerative disease is huntington's disease.

Advantageous effects

The compounds of the present invention have the effect of reducing the level of polyQ-GFP fusion proteins with longer polyQ in cells without reducing the level of polyQ-GFP fusion proteins with shorter polyQ. Therefore, the compound of the present invention can reduce the level of polyQ protein having a longer polyQ in cells or tissues, thereby having a preventive or therapeutic effect on a polyQ protein-related disease.

The inventors have further found that the compounds of the invention have an unexpected regulatory effect on mHTT levels in cells and have good safety profile. Administration of the compounds of the invention improves survival and crawling ability of the HD model drosophila. The administration of the compounds of the invention by either intracerebroventricular or intraperitoneal injection reduces mHTT levels in the cerebral cortex and striatum of HD model mice in an allele-selective manner without affecting the levels of wtHTT which has important physiological functions and improves the behavioral deficits of the mice. In HD patients induced stem cell (iPSC) differentiation neurons, the compounds of the invention reduced mHTT levels, rescued disease-related phenotypes, and delayed neuronal apoptosis. Therefore, the compound of the invention has good selectivity, treatment effect and safety, is easy to permeate BBB and is beneficial to oral administration.

In addition, the compounds of the present invention significantly reduce the level of variant ATXN3 protein in cells and can therefore be used for the treatment or prevention of spinocerebellar ataxia type 3.

Therefore, the compound can treat polyQ protein related diseases, has the advantages of high selectivity and good safety, and has a good prospect of being developed into oral medicaments.

Examples

Unless otherwise specified, the instruments and reagents used herein are commercially available.

In all statistical analyses herein, denotes p < 0.05; denotes p < 0.01; denotes p <0.001, denotes p < 0.0001. For the comparison between the two groups, the statistical analysis method used was the two-tailed unpaired t-test. For comparison among three or more groups, the method used is two-tailed one-way anova under the condition of only one variable influence, and the method used is two-tailed two-way anova under the condition of two variable influences.

Abbreviations

Test materials, reagents and method steps

Compound (I)

Compound 1: ispinesib, PubChem CID:6851740, available from seleck, catalog No. S1452;

compound 2: PubChem CID 5398649, available from ChemDiv, catalog No. D715-2435;

compound 3: semaxanib, available from Selleck, CAS No. 194413-58-6;

compound 4: su9516, available from Selleck, CAS No. 377090-84-1;

compound 5: commercially available from TargetMol, CAS No. 842-01-3;

compound 6: commercially available from ChemDiv;

compound 7: commercially available from ChemDiv, CAS No. 130181-72-5.

Antibodies

Antibodies for western blot, HTRF and/or immunofluorescence/immunohistochemistry are as follows: HTT antibodies 2B7(Weiss et al. anal Biochem 2009,395,8-15), 4C9, ab1(Sapp et al. J Biol Chem 2012,287, 13487-; antibody S830 for immunostaining detection of HTT aggregates was obtained from doctor Gillian Bates; other antibodies were purchased from Millipore, Sigma, etc.

Preparation and validation of recombinant human full-length HTT protein

(1) Has (CAG)₂₃Or (CAG)₇₃The human HTT gene (GenBank: NM-002111.8) of (5) was synthesized de novo by Genewiz Inc. The human HTT gene was cloned into a modified pCAG vector (from Addgene) with an N-terminal protein a tag.

(2) Plasmids were transfected into human embryonic kidney E293 cells for expression using polyethyleneimine (PEI, from Polysciences, 23966). IgG monoclonal-agarose (Smart-lifesciences, SA030010) purification, digestion with TEV protease to remove the protein A tag, and further purification of the protein using Mono Q and Superose 6(5/150GL) columns from GE healthcare. Verified by coomassie blue staining and western blotting.

Cells for assays

Primary cultured cortical neurons: hdh^Q140/Q7And Hdh^Q7/Q7The brain of the newborn mouse (P0) was dissected, digested, dissociated and cultured.

Some primary patient fibroblasts and wild-type cells were from HD patients of the montmorillonoid huntington disease family (Q47, Q55) and healthy controls (WT, Q19). The SCA3 cell line was from a patient (Q74). The HD Q68 fibroblast Cell line was from Coriell Cell repositides (Camden, NJ, USA). Immortalized fibroblasts and iPS cells (ipscs) were prepared from primary fibroblasts. HEK293T cells were from ATCC.

Animals for testing

Fruit fly of Huntington's disease

The nervous system driving line elav-GAL4(c155), the HTT expressing lines UAS-fl-HTT-Q16 and UAS-fl-HTT-Q128 were from Blomington Drosophila Stock Center of Indiana university (http:// flytoscks. bio. indiana. edu /), and were maintained in an incubator at 25 ℃.

Transgenic drosophila expressing the full-length human HTT protein (Q16) or (Q128) in the nervous system driven by elav-GAL4 were obtained by crossing elav-GAL4 virgins with UAS-fl-HTT-Q16 or UAS-fl-HTT-Q128 male drosophila.

Mouse with Huntington's disease

Mice expressing wild type HTT gene (Hdh)^Q7/Q7) From Marian Difiglia laboratory, general Hospital, Harvard university. Q140 knock-in heterozygous mice (Hdh) were prepared according to the methods of the prior art (Menalled et al, J Comp Neurol,2003,465:11-26)^Q140/Q7)。

Protein analysis

Homogeneous time-resolved fluorescence (HTRF) analysis: the original lysis buffer PBS + 1% (v/v) Triton X-100+1 XcOmplete was used^TMProtease enzymeCell or tissue lysates were diluted with inhibitors, samples lysed, and assayed in HTRF assay buffer (50mM NaH)₂PO₄400mM NaF, 0.1% BSA, 0.05% (v/v) Tween-20, 1% (v/v) Triton X-100, pH 7.4) diluted designated antibody pairs. In HTRF buffer, the concentration of donor antibody was 0.023 ng/. mu.L and the concentration of acceptor antibody was 1.4 ng/. mu.L.

Determination of the amount of protein: the amount of protein was determined by the method described above. Background correction was performed by blank samples. The concentration of protein was determined for all samples to correct the amount of protein. Different protein concentrations or cell numbers per well were determined to ensure that the signal was in the linear range.

Cell analysis

Immunofluorescence: after washing, fixing, permeabilizing and blocking the cells, they were incubated overnight with primary antibody at 4 ℃ and then three times with blocking buffer and incubated with secondary antibody for 1 hour at room temperature. After staining with DAPI, mounting, imaging with Zeiss Axio Vert a1 confocal microscope, and analysis of TUBB3 or co-localisation with ImageJ.

EXAMPLE 1 Effect of Compounds on protein levels with longer glutamine repeat regions

The inventors used HEK293T cells exogenously expressing polyQ-GFP fusion proteins (Q72-GFP or Q25-GFP) to test compounds capable of controlling the levels of proteins containing longer glutamine repeats in the cells.

The method comprises the following steps:

(1) PolyQ-GFP sequences (expressing Met-polyQ-GFP, where polyQ is Q72 or Q25) were synthesized de novo and subcloned into pcDNA vectors. The cDNA was forward transfected into HEK293T cells (ATCC, CRL-3216) to give Q72-GFP expressing cells and Q25-GFP expressing cells, respectively.

(2) Cells were treated with compound 1 at a concentration of 100nM, or compound 2 at a concentration of 50nM, and polyQ-GFP levels were measured by Incucyte detection fluorescence counts 2 days later (fig. 1). It was observed that compound 1 and compound 2 were effective in reducing the levels of the protein containing the longer glutamine repeat region (Q72-GFP) but not the shorter glutamine repeat region (Q25-GFP) in HEK293T cells. This result indicates that the compounds of the present invention can selectively reduce the level of a polyQ-aberrantly amplified protein in a cell.

EXAMPLE 2 Effect of Compounds with full-Length mHTT

2.1 OI-RD detection of affinity Activity of Compounds with full-Length HTT

Compound chips were prepared using a contact microarray spot printer (SmartArrayer 136, CapitalBio Corporation) following the methods of the prior art (Zhu et al, Sensors (Basel)2016,16(3), 378; Fei et al, J Biomed Opt 2010,15(1), 016018). Each compound was spotted in triplicate. Affinity reaction parameters of compounds with full-length mHTT (flHTT-Q73) and full-length HTT-Q23(flHTT-Q23) were measured using OI-RD (FIG. 2). The compounds did not bind with affinity to fl-HTT-Q23, and the association, dissociation and equilibrium dissociation constants of the affinity reaction with flHTT-Q73 are shown in Table 1.

TABLE 1 Association, dissociation and equilibrium dissociation constants for affinity reaction of compounds with flHTT-Q73

2.2 MST detection of affinity Activity of Compounds with full-Length HTT

The affinity activity of the compounds to the full-length HTT was verified with a microcalorimeter (MST, wherein Monolith nt.115 instrument from NanoTemper Technologies) (fig. 3). The reaction buffer was 20mM HEPES, pH 7.4,150mM NaCl, protein concentration 500 nM. The compounds of the invention do not bind with affinity to fl-HTT-Q23. K of Compound 1 with flHTT-Q73_d0.13. mu.M; k of Compound 2 with flHTT-Q73_dIt was 2.82. mu.M.

In summary, both OI-RD and MST assays indicate that the compounds of the invention selectively bind affinity to flHTT-Q73. This conclusion is in-line with the phenomenon of selective reduction of protein levels in HEK293T cells containing the longer glutamine repeat region by compounds, suggesting that the effect of compounds on such protein levels may be achieved by selective binding to proteins containing the longer glutamine repeat region.

EXAMPLE 3 Effect of Compounds on cortical neurons in HD model mice

3.1 Effect on mHTT and wtHTT levels

(1) Treatment of primary cultured Hdh with Compounds^Q140/Q7And Hdh^Q7/Q7Mouse cortical neuronal cells, mHTT, wtHTT levels were detected 2 days later by western blot (2166 antibody) (fig. 4 and 5). Compounds 1 and 2 knock Q140 gene into heterozygous mice (Hdh)^Q140/Q7) Cortical neuron mHTT levels decreased while wtHTT levels were barely affected. Compound 1 does not reduce Hdh^Q7/Q7Mouse cortical neuron wtHTT levels.

(2) mHTT was detected with anti-polyQ antibodies MW1 and 3B5H10, and a band of a protein having a smaller molecular weight was observed, and as a result, no increase in N-terminal fragment of mHTT was observed (fig. 6). The decrease in mHTT levels detected was not due to an increase in site-specific cleavage.

3.2 cytotoxicity assays

The Hdh treated with the indicated compounds was determined by CellTiter-glo (Promega, catalog number G7570) according to the protocol provided in the kit^Q140/Q7Viability of mouse cortical neuronal cells (figure 7).

Compounds of the invention were tested for Hdh at the test concentrations indicated at 3.1^Q140/Q7Mouse cortical neurons were not cytotoxic. The detected reduction in mHTT levels was not due to neuronal cell loss.

In conclusion, the compounds of the invention can reduce the mHTT level in cells in an allele selective manner, have no cytotoxicity and have better safety.

EXAMPLE 4 Effect of Compounds on the mHTT, wtHTT levels in fibroblasts from Huntington patients

4.1 Effect on mHTT and wtHTT levels of Primary fibroblasts in HD patients

Preliminary experiments were performed to determine that compounds showed the best effect of reducing mHTT levels at 100nM concentration.

The test method comprises the following steps: fibroblasts of HD patients (Q49, Q55, Q68) were treated with a compound at a concentration of 100nM, and mHTT (antibody pair: 2B7/MW1) and total HTT (antibody pair: 2B7/2166) were detected by HTRF 2 days later, with the results shown in FIG. 8. Decreased mHTT levels were observed on primary fibroblasts (Q49, Q55, Q68) of HD patients. No reduction in HTT levels was observed on wild type primary fibroblasts.

4.2 Effect on immortalized fibroblast mHTT levels in HD patients

The effect of the compounds of the invention on the level of mHTT of fibroblasts from immortalized HD patients was examined by HTRF (antibody pair: 2B7/MW1) using a test method similar to that described in 4.1 (FIG. 9). A decrease in mHTT levels was observed on fibroblasts of immortalized HD patients. Figure 10 shows that compound 2 reduces mHTT levels in fibroblasts of immortalized HD patients at different doses.

EXAMPLE 5 Effect of Compounds on the Induction of Stem cell differentiation neuronal mHTT levels and neuronal apoptosis in HD patients

5.1 Effect on mHTT and wtHTT levels

Experimental materials:

the effect of compound 1 and compound 2 on mHTT levels in neurons inducing stem cell (iPSC) differentiation (Q47) in HD patients was examined by HTRF (antibody pair: 2B7/MW1) using a similar assay method as described in 4.1 (figure 11). A decrease in mHTT levels was observed on the neuronal cells induced stem cell (iPSC) differentiation in HD patients. In the same way, it was observed that compound 3, compound 4, compound 5, compound 6, compound 7 decreased the mHTT level in the neuronal cells inducing stem cell (iPSC) differentiation in HD patients. The compounds of the invention rescue disease-associated phenotypes in neuronal cells induced stem cell (iPSC) differentiation in HD patients.

5.2 Effect on neuronal apoptosis

(1) Immunostaining

Treatment of HD patients with 100nM compound 1 or 50nM compound 2 induced stem cell (iPSC) differentiated neurons (Q47). Cells were stressed 1 day after compound treatment (BDNF depletion).

The neuron specific tubulin marker TUBB3 was stained with DAPI. Neuronal apoptosis was analyzed by normalizing TUBB3 signal coverage area by nuclear count and normalizing data against wild type (fig. 12).

(2) Caspase-3 activity assay

After BDNF was removed, HD neuronal arrest was observed and neuronal contractions occurred.

Active caspase-3 was detected with NucView 488(Biotium, 30029). After BDNF removal, images were captured every 3 hours in the incubator using incuyte (Essen Bioscience, Incucyte FLR) and analyzed with incuyte 2011A software. Three batches were tested in total and the results were consistent (FIG. 13). Compounds 1 and 2 had significant improvement in the halting progression of HD neurons and neuronal contraction following BDNF ablation.

EXAMPLE 6 Effect of Compounds on Huntington's disease Drosophila

6.1 Effect on mHTT levels

The experimental method comprises the following steps:

q128 fruit flies and Q16 fruit flies were randomly divided into a negative control group and a positive drug group (Compound 1, Compound 2), respectively, and 75 flies were added to each group. The negative control group is administered with the corresponding solvent DMSO, and the positive drug group is administered with the corresponding positive drug.

The fruit flies were kept in standard food at 25 ℃. Freshly hatched fruit flies were transferred to vials of the dispensed diet containing either positive drug (10 μ M in 400 μ L DMSO) or control DMSO, with corn diet changed every other day.

Feeding continuously for 6 days. Drosophila head proteins were extracted on day 7 and mHTT levels were measured by HTRF (antibody pair: 2B7/MW1), with each sample comprising 5 Drosophila head extracted proteins (FIG. 14). Compounds 1 and 2 reduced the level of transgenic drosophila mHTT expressing the full-length protein of human HTT (Q128).

6.2 Effect of Compounds on survival

75 age-matched virgins were placed in empty plastic vials containing standard food and the viability of each vial was recorded daily to measure its life (FIG. 15). The survival rate of the Q128 drosophila positive drug group was improved relative to the control group.

6.3 Effect on crawling ability

15 age-matched virgins were placed in empty bottles and tapped so that they were at the bottom of the bottle. The percentage of flies that climbed 7 cm high after 15 seconds was recorded. An average of five observations was recorded daily for each vial, and data from multiple vials containing different batches of fruit flies was recorded and analyzed.

The results are shown in FIG. 16. The numbers in parentheses indicate the number of vials tested. The crawling ability of the Q128 drosophila positive drug group was improved relative to the control group.

In the above experiments 6.2 and 6.3, no effect of the compound on Q16 drosophila was observed.

EXAMPLE 7 Effect of Compounds on HD model mice

Experimental animals: mice were housed in groups in individual ventilated cages with a 12-hour light/dark cycle, with a maximum of 5 adult mice per cage.

7.1 Effect of intracerebroventricular injection of Compounds on cortical mHTT and wtHTT levels in HD mice

Experimental animals: hdh^Q140/Q7Mice (3 months old), 4 per group;

the experimental method comprises the following steps: an intracerebroventricular injection was performed once daily, and 2. mu.L of artificial cerebrospinal fluid (ACSF: 1mM glucose, 119mM NaCl, 2.5mM KCl, 1.3mM MgSO) containing a compound at a concentration of 25. mu.M was administered per injection₄，2.5mM CaCl₂，26.2mM NaHCO₃，1mM NaH₂PO₄). mu.L of ACSF containing equal amounts of DMSO was used as a control.

10 days after injection, mouse cortical neuronal proteins were extracted and mHTT and wtHTT levels were detected by western blot (2166 antibody). The test was repeated 3 times for each sample and the average value was calculated (fig. 17). Compound 2 significantly reduced the cortical mHTT levels in hdhdhq 140/Q7 mice and exhibited mHTT selectivity relative to wtHTT.

7.2 Abdominal CavityAdministration of Compound 2 by injection

The experimental method comprises the following steps: DMSO for compound or control was diluted to 0.05. mu.g/. mu.L with 0.9% NaCl intravenous infusion solution, and was injected intraperitoneally once a day (0.5mg/kg) for 14 days, followed by tissue extraction or behavioral experiments.

In vivo compound detection of intraperitoneal injection of mouse brain tissue: 2 hours after intraperitoneal injection of DMSO or compound into mice, brains were dissected, weighed, compounds in brain tissue extracted, and LC-MS/MS analysis was performed using UPLC-MS (Acquity ultra performance liquid chromatography system, Acquity UPLC BEH C18(1.7 μm, 2.1X 50mm) column and a Xevo TQ-S mass spectrometer, Waters Corporation, Milford, MA, USA). The level of compound 2 reaching brain tissue was 9.48 ng/g. No mass spectrum signal was observed for the compound in the control (DMSO) group. The compound of the invention can be successfully delivered to the brain of a mouse through the BBB, and is beneficial to oral administration of the compound.

7.3 Effect of intraperitoneal injection of Compounds on cortical and striatal mHTT and wtHTT levels in HD mice

(1) Taking Hdh^Q140/Q7Mice (5 months old), 13 in total, were divided into 2 groups

The administration was carried out according to the procedure described in 7.2. Intraperitoneal injections were performed once a day, and 14 days after the injections, proteins were extracted and mHTT and wtHTT levels were detected by western blotting (fig. 18).

(2) Taking Hdh^Q140/Q7Mice (10 months old), 13 in total, 6-7 per group.

Mouse brain striatal neuronal proteins were administered and extracted as described above and mHTT and wtHTT levels were detected by western blot (fig. 19).

(3) Hdh was detected by dot-blot assay (antibody: 4C9, bar graph showing the results of duplicate assays) and HTRF (antibody pair: 4C9/4C9)^Q140/Q7mHTT aggregates in mouse cortex. Each mouse was repeatedly sampled two to three times on average (fig. 20). No increase in mHTT aggregates was observed. The decrease in mHTT levels in the compound 2 dosed group was not due to a change in mHTT solubility.

In conclusion, intraperitoneal administration of compound 2 reduced cortical and striatal mHTT levels in HdhQ140/Q7 mice and showed mHTT selectivity over wtHTT. Therefore, the compound 2 has a prospect of being developed into oral medicaments.

7.4 Effect of intraperitoneal injection of Compound 2 on behavioral deficits in HD mice

Experimental animals: hdh^Q140/Q7Mice, 32 in total, were divided into 2 groups; (Hdh)^Q7/Q7) The total number of 28 mice was divided into 2 groups

The experimental method comprises the following steps: the administration was carried out according to the procedure described in 7.2. Intraperitoneal injection is carried out once a day, and behavioral experiments are carried out 14 days after injection.

All behavioral experiments were performed during the light phase. All mice were kept in the behavioral testing room for one hour under dim red light before starting the experiment.

All behavioral experiments were performed during the light phase. All mice were kept in the behavioral testing room for one hour under dim red light before starting the experiment.

Rotating rod test: mice were pre-trained for 3 consecutive days (spinning on a rotarod at 4rpm for 2 minutes). The mice were then tested for 5 days at an acceleration rate of 4 to 40rpm over 2 minutes. The results of each experiment were recorded as the time on the rod (time on the rotating rod) until dropping off the rod or until the end of the task. Each test included three replicates with test intervals of 60 minutes to reduce stress and fatigue. The average of three trials per mouse was analyzed (fig. 21 a).

And (3) balance beam test: a rod 2 cm thick and 100 cm in total length with graduations, both sides of which were suspended from the platform. The starting point is provided with a bright light, and the end point is provided with a dark box filled with food. The total time each mouse took across the equalizer beam was recorded (fig. 21 b). The compound can improve the Huntington's disease-related behavior defect of HD model mice, and has no influence on wild type mice.

Example 8 Effect of Compounds on the protein level of ATXN3 in fibroblasts from spinocerebellar ataxia type 3 patients fibroblasts (Q74), wild type cells (Q27) from SCA3 patients were treated with compound 1 at a concentration of 100nM or compound 2 at a concentration of 50nM, and the levels of variant ATXN3 protein (ATXN3-Q74), wild type ATXN3 protein (ATXN3-Q27) were determined by Western blotting 2 days later (FIG. 22). Reduced levels of variant ATXN3 protein were observed on fibroblasts (Q74) from patients with SCA3, whereas no reduction in wild-type ATXN3 protein levels was observed, therefore the compounds are useful for treating SCA 3.

Claims (15)

1. Use of a compound of formula (II), or a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite or prodrug thereof, in the manufacture of a medicament for the prevention or treatment of a polyQ-related neurodegenerative disease

Wherein:

ring A is a benzene ring;

ring B is a saturated or unsaturated five-or six-membered heterocyclic ring, phenyl ring, or six-membered heteroaromatic ring containing 1,2, or 3 heteroatoms each independently selected from N, O and S;

c ring is selected from C_6-10Aryl and five-to ten-membered heteroaryl, optionally substituted with one or more substituents each independently selected from R^X1Substituted with a group of (1);

L¹is a bond, or is C₁-C₆A hydrocarbon chain;

or, C ring is absent, and L¹Is absent;

R¹is ═ Y, where Y is O OR S, OR is OR⁷；

R²At each occurrence is independently selected from H, halogen, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, C_3-10Cycloalkyl radical, C_3-10Cycloalkyl radical-C_1-4Alkyl, three-to ten-membered heterocyclyl-C_1-4Alkyl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, ═ O, ═ S, ═ NR^a1、-OR^a1、-SR^a1、-NR^a1R^b1、-C(＝O)OR^a1、-C(＝O)NR^a1R^b1、-C(＝O)R^a1、-S(＝O)₂OR^a1、-S(＝O)₂R^a1、-S(＝O)₂NR^a1R^b1、-S(＝O)R^a1、-C(＝S)OR^a1、-C(＝S)NR^a1R^b1、-C(＝S)R^a1、-P(＝O)(OR^a1)OR^b1、-C(＝NR^a1)NR^b1R^c1-OCN, -SCN, -N ═ C ═ O, -NCS, where the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl or heteroaryl are optionally substituted with one or more substituents selected from halo, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, ═ O, ═ S, -OR^a2、-SR^a2、-NR^a2R^b2、-C(＝O)OR^a2、-C(＝O)NR^a2R^b2、-C(＝O)R^a2、-S(＝O)₂OR^a2、-S(＝O)₂R^a2、-S(＝O)₂NR^a2R^b2、-S(＝O)R^a2and-C (═ NR)^a2)NR^b2R^c2Substituted with the substituent(s);

or, two adjacent R²Together with the C atom to which they are attached form a five-to seven-membered lactam ring, optionally substituted by one or more groups selected from halogen, C_1-6Alkyl substituent substitution;

R³、R⁴、R⁵、R⁶each independently selected from H and R^X2；

Or, R⁴And R⁵Together with the C atom to which they are attached form a five-to seven-membered heterocyclic ring containing 1 or 2 heteroatoms each independently selected from N, O and S, and optionally substituted with one or more substituents selected from halogen and C_1-6Alkyl substituent substitution;

R^X1and R^X2Each occurrence independently selected from halogen, -NO₂、-CN、C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, -OR⁷、-SR⁷、-NR⁷R⁸、-C(＝O)OR⁷、-C(＝O)NR⁷R⁸、-OC(＝O)R⁷、-NC(＝O)R⁷R⁸、-C(＝O)R⁷、-S(＝O)₂OR⁷、-S(＝O)₂R⁷、-S(＝O)₂NR⁷R⁸、-OS(＝O)₂R⁷、-NS(＝O)₂R⁷R⁸、-S(＝O)R⁷Wherein said alkyl, alkenyl or alkynyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -SH, -S (C)_1-6Alkyl), -S (C)_3-6Cycloalkyl), -S (C)_1-4alkylene-C_3-6Cycloalkyl), -S (three-to seven-membered heterocyclyl), -S (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂O, -COOH and C_1-6Alkyl substituent substitution;

R⁷、R⁸each occurrence is independently selected from H, C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl radical, C_6-10aryl-C_1-4Alkyl, wherein the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl or aryl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-COOH、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl) substituted with a substituent;

R^a1、R^b1、R^c1、R^a2、R^b2、R^c2each occurrence is independently selected from H, C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl radical, C_6-10Aryl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, -OR^Y1、-SR^Y1、-NR^Y1R^Y2、-C(＝O)OR^Y1、-C(＝O)NR^Y1R^Y2、-C(＝O)R^Y1、-S(＝O)₂OR^Y1、-S(＝O)₂R^Y1、-S(＝O)₂NR^Y1R^Y2、-S(＝O)R^Y1Wherein said alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl OR heteroaryl is optionally substituted with one OR more substituents selected from halogen, ═ O, ═ S, -OR^Y3、-SR^Y3、-NR^Y3R^Y4、-C(＝O)R^Y3、-C(＝O)OR^Y3and-C (═ O) NR^Y3R^Y4Substituted with the substituent(s);

R^Y1、R^Y2、R^Y3、R^Y4each occurrence is independently selected from H, C_1-8Alkyl radical, C_3-10Cycloalkyl radical, C_3-10Cycloalkyl radical-C_1-4Alkyl, three-to ten-membered heterocyclyl-C_1-4Alkyl radical, C_6-10Aryl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, wherein the alkyl, alkenyl, alkynyl, cyclicThe alkyl, heterocyclyl, aryl or heteroaryl group being optionally substituted by one or more groups selected from halogen, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, -OH, -SH, -NH₂Substituted with a substituent of ═ O and-COOH;

n is 1 or 2;

with the proviso that the compound of formula (I) does not include the following structure:

wherein R is³Is selected from-O (C)_1-6Alkyl), -O (C)_1-4alkylene-C_6-10Aryl groups); r¹⁰Is H;

and the compounds of formula (I) do not include the following structure:

wherein R is⁴is-O (C)_1-6Alkyl groups); r⁵Is halogen; r¹⁰Is CF₃；

And the compounds of formula (I) do not include the following structure:

wherein R is⁴Is selected from-O (C)_1-6Alkyl), -O (C)_1-4alkylene-C_6-10Aryl groups);

ring C being C_6-10Aryl, optionally substituted with one or more substituents each independently selected from R^X1Substituted with a group of (1);

and the compounds of formula (I) do not include the following structure:

wherein R is⁴Selected from-OH, -O (C)_1-6Alkyl groups); r⁵Is halogen; the C ring is a five-to ten-membered heteroaryl group optionally substituted with one or more substituents each independently selected from R^X1Is substituted with a group (b).

2. The use of claim 1, wherein the compound of formula (I) has the structure of formula (II) below:

wherein:

y is O or S;

the C ring is a five-to seven-membered heteroaryl group optionally substituted with one or more substituents each independently selected from R^X1Substituted with a group of (1);

R²selected from H, C_1-8An alkyl group;

L¹is a bond, or is C₁-C₆A hydrocarbon chain;

R³、R⁴、R⁵、R⁶each independently selected from H and R^X2；

Wherein R is^X1、R^X2As defined in claim 1.

3. The use of claim 1, wherein the compound of formula (I) has the structure of formula (IV) below:

wherein:

y is O or S;

x is O, NH or S;

R⁹selected from H, halogen, C_1-6Alkyl radical, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl, -OR^a1、-SR^a1、-NR^a1R^b1、-C(＝O)OR^a1、-C(＝O)NR^a1R^b1、-C(＝O)R^a1、-S(＝O)₂OR^a1、-S(＝O)₂R^a1、-S(＝O)₂NR^a1R^b1、-S(＝O)R^a1Wherein said alkyl, cycloalkyl or heterocyclyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OR^a2、-SR^a2、-NR^a2R^b2、-C(＝O)OR^a2、-C(＝O)NR^a2R^b2、-C(＝O)R^a2、-S(＝O)₂OR^a2、-S(＝O)₂R^a2、-S(＝O)₂NR^a2R^b2and-S (═ O) R^a2Substituted with the substituent(s); wherein R is^a1、R^b1、R^a2、R^b2As defined in claim 1;

R¹⁰selected from H, halogen, C_1-6Alkyl radical, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4An alkyl group;

or, R⁹And R¹⁰Together with the C atom to which they are attached form a five-to seven-membered lactam ring, optionally substituted by one or more groups selected from halogen, C_1-6Alkyl substituent substitution;

R³selected from H, halogen, C_1-6Alkyl, -OH, -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Wherein said alkyl is optionally substituted by one or more groups selected from halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Substituted with the substituent(s);

R⁴selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OR⁷、-SR⁷、-NR⁷R⁸；R⁷、R⁸Each occurrence is independently selected from H, C_1-6Alkyl, wherein the alkyl is optionally substitutedOne or more selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-COOH、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl) substituted with a substituent; wherein R is⁷、R⁸As defined in claim 1;

R⁵selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl), wherein the alkyl is optionally substituted with one or more substituents selected from the group consisting of halogen, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl) substituted with a substituent;

or, R⁴And R⁵Together with the C atom to which they are attached form a five-to seven-membered heterocyclic ring containing 1 or 2 heteroatoms each independently selected from N, O and S, and optionally substituted with one or more substituents selected from halogen and C_1-6Alkyl substituent substitution;

R⁶selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -O (benzyl), -SH, -S (C)_1-6Alkyl), -S (benzyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂-NH (benzyl), wherein said alkyl or benzyl is optionally substituted by one or more groups selected from haloElement, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂Substituted with the substituent(s);

provided that when R is⁴is-O (C)_1-6Alkyl) and R⁵When it is halogen, R¹⁰Is not CF₃。

4. The use of claim 1, wherein the compound of formula (I) has the structure of formula (VI):

wherein:

ring B is a saturated or unsaturated six-membered heterocyclic ring containing 1,2, or 3 heteroatoms each independently selected from N, O and S;

ring C being C_6-10Aryl, optionally substituted with one or more substituents each independently selected from R^X1Substituted with a group of (1);

L¹is a bond, or is C₁-C₆A hydrocarbon chain;

R²selected from H, halogen, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, C_3-10Cycloalkyl radical, C_3-10Cycloalkyl radical-C_1-4Alkyl, three-to ten-membered heterocyclyl-C_1-4Alkyl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, ═ O, ═ S, ═ NR^a1、-OR^a1、-SR^a1、-NR^a1R^b1、-C(＝O)OR^a1、-C(＝O)NR^a1R^b1、-C(＝O)R^a1、-S(＝O)₂OR^a1、-S(＝O)₂R^a1、-S(＝O)₂NR^a1R^b1、-S(＝O)R^a1、-C(＝S)OR^a1、-C(＝S)NR^a1R^b1、-C(＝S)R^a1、-P(＝O)(OR^a1)OR^b1、-C(＝NR^a1)NR^b1R^c1-OCN, -SCN, -N ═ C ═ O, -NCS, where the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl or heteroaryl are optionally substituted with one or more substituents selected from halo, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, ═ O, ═ S, -OR^a2、-SR^a2、-NR^a2R^b2、-C(＝O)OR^a2、-C(＝O)NR^a2R^b2、-C(＝O)R^a2、-S(＝O)₂OR^a2、-S(＝O)₂R^a2、-S(＝O)₂NR^a2R^b2、-S(＝O)R^a2and-C (═ NR)^a2)NR^b2R^c2Substituted with the substituent(s);

R³、R⁴、R⁵、R⁶each independently selected from H and R^X2；

Or, R⁴And R⁵Together with the C atom to which they are attached form a five-to seven-membered heterocyclic ring containing 1 or 2 heteroatoms each independently selected from N, O and S, and optionally substituted with one or more substituents selected from halogen and C_1-6Alkyl substituent substitution;

R^X1and R^X2Each occurrence independently selected from halogen, -NO₂、-CN、C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, -OR⁷、-SR⁷、-NR⁷R⁸、-C(＝O)OR⁷、-C(＝O)NR⁷R⁸、-OC(＝O)R⁷、-NC(＝O)R⁷R⁸、-C(＝O)R⁷、-S(＝O)₂OR⁷、-S(＝O)₂R⁷、-S(＝O)₂NR⁷R⁸、-OS(＝O)₂R⁷、-NS(＝O)₂R⁷R⁸、-S(＝O)R⁷Wherein said alkyl, alkenyl or alkynyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -O (C)_3-6Cycloalkyl), -O (C)_1-4alkylene-C_3-6Cycloalkyl), -O (three-to seven-membered heterocyclyl), -O (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -SH, -S (C)_1-6Alkyl), -S (C)_3-6Cycloalkyl), -S (C)_1-4alkylene-C_3-6Cycloalkyl), -S (three-to seven-membered heterocyclyl), -S (C)_1-4Alkylene) - (three-to seven-membered heterocyclyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C_3-6Cycloalkyl), -N (C)_3-6Cycloalkyl radicals₂、-NH(C_1-4alkylene-C_3-6Cycloalkyl), -N (C)_1-4alkylene-C_3-6Cycloalkyl radicals₂NH (three-to seven-membered heterocyclic group), -N (three-to seven-membered heterocyclic group)₂、-NH(C_1-4Alkylene-three-to seven-membered heterocyclyl), -N (C)_1-4Alkylidene-three to seven membered heterocyclic groups)₂O, -COOH and C_1-6Alkyl substituent substitution;

R⁷、R⁸each occurrence is independently selected from H, C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl, wherein the alkyl, alkenyl, alkynyl, cycloalkyl or heterocyclyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -O (C)_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-COOH、-C(＝O)O(C_1-6Alkyl), -C (═ O) NH (C)_1-6Alkyl), -C (═ O) N (C)_1-6Alkyl radical)₂、-OC(＝O)(C_1-6Alkyl), -NHC (═ O) (C)_1-6Alkyl), -C (═ O) (C)_1-6Alkyl) substituted with a substituent;

R^a1、R^b1、R^c1、R^a2、R^b2、R^c2each occurrence is independently selected from H, C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, C_3-6Cycloalkyl radical, C_3-6Cycloalkyl radical-C_1-4Alkyl, three-to seven-membered heterocyclyl-C_1-4Alkyl radical、C_6-10Aryl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, -OR^Y1、-SR^Y1、-NR^Y1R^Y2、-C(＝O)OR^Y1、-C(＝O)NR^Y1R^Y2、-C(＝O)R^Y1、-S(＝O)₂OR^Y1、-S(＝O)₂R^Y1、-S(＝O)₂NR^Y1R^Y2、-S(＝O)R^Y1Wherein said alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl OR heteroaryl is optionally substituted with one OR more substituents selected from halogen, ═ O, ═ S, -OR^Y3、-SR^Y3、-NR^Y3R^Y4、-C(＝O)R^Y3、-C(＝O)OR^Y3and-C (═ O) NR^Y3R^Y4Substituted with the substituent(s);

R^Y1、R^Y2、R^Y3、R^Y4each occurrence is independently selected from H, C_1-8Alkyl radical, C_3-10Cycloalkyl radical, C_3-10Cycloalkyl radical-C_1-4Alkyl, three-to ten-membered heterocyclyl-C_1-4Alkyl radical, C_6-10Aryl radical, C_6-10aryl-C_1-4Alkyl, five-to ten-membered heteroaryl-C_1-4Alkyl, wherein the alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、C_1-8Alkyl radical, C_2-8Alkenyl radical, C_2-8Alkynyl, -OH, -SH, -NH₂Substituted with a substituent of ═ O and-COOH;

a ring, R¹As defined in claim 1;

with the proviso that the compound of formula (VI) does not include the following structure:

wherein R is⁴Is selected from-O (C)_1-6Alkyl), -O (C)_1-4alkylene-C_6-10Aryl).

5. The use of claim 1, wherein the B ring is dihydropyrimidine.

6. The use of claim 1, wherein the B ring is 2H-pyran or 4H-pyran; 2H-pyran is preferred.

7. The use of any one of claims 1-6, wherein R¹Is ═ O.

8. Use according to claim 5, wherein A-B is a ring system

9. Use according to claim 6, wherein A-B is a ring system

10. The use of any one of claims 4-9, wherein L¹Is a bond, or is C₁-C₂A hydrocarbon chain.

11. Use according to any one of claims 4 to 10 wherein ring C is phenyl optionally substituted with 1,2, 3,4 or 5 each independently selected from R^X1Substituted with a group of (1);

preferably the C ring is

Wherein R is¹⁴、R¹⁵、R¹⁶、R¹⁷、R¹⁸Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl radical, C_2-6Alkenyl radical, C_2-6Alkynyl, -OR⁷、-SR⁷、-NR⁷R⁸、-C(＝O)OR⁷、-C(＝O)NR⁷R⁸、-OC(＝O)R⁷、-NC(＝O)R⁷R⁸、-C(＝O)R⁷、-S(＝O)₂OR⁷、-S(＝O)₂R⁷、-S(＝O)₂NR⁷R⁸、-OS(＝O)₂R⁷、-NS(＝O)₂R⁷R⁸、-S(＝O)R⁷

Preferably, R¹⁴、R¹⁵、R¹⁶、R¹⁷、R¹⁸Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OR⁷、-NR⁷R⁸、-C(＝O)OR⁷、-C(＝O)NR⁷R⁸、-OC(＝O)R⁷、-NC(＝O)R⁷R⁸、-C(＝O)R⁷；

More preferably, R¹⁴、R¹⁵、R¹⁷、R¹⁸Each independently selected from H, halogen, -NO₂、-CN、C_1-6Alkyl, -OH, -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂、-NH(C＝O)(C_1-6Alkyl groups); r¹⁶Is H or-OCH₃。

12. The use of claim 4, wherein the compound of formula (VI) has the structure of formula (IX) as follows:

wherein:

R¹⁹each occurrence independently selected from halogen, -NO₂、-CN、C_1-6Alkyl, -OR⁷、-SR⁷、-NR⁷R⁸、-C(＝O)OR⁷、-C(＝O)NR⁷R⁸、-OC(＝O)R⁷、-NC(＝O)R⁷R⁸、-C(＝O)R⁷、-S(＝O)₂OR⁷、-S(＝O)₂R⁷、-S(＝O)₂NR⁷R⁸、-OS(＝O)₂R⁷、-NS(＝O)₂R⁷R⁸、-S(＝O)R⁷Wherein said alkyl is optionally substituted with one or more substituents selected from halogen, -NO₂、-CN、-OH、-O(C_1-6Alkyl), -NH₂、-NH(C_1-6Alkyl), -N (C)_1-6Alkyl radical)₂-COOH;

m is 0,1, 2,3, 4 or 5;

R²、R³、R⁴、R⁵、R⁶as defined in claim 4.

13. Use according to any one of claims 1 to 12, wherein the compound is selected from

14. Use of a pharmaceutical composition for the manufacture of a medicament for preventing or treating a PolyQ-related neurodegenerative disease; the pharmaceutical composition comprises a compound of formula (I), formula (II), formula (III'), formula (IV), formula (V), formula (VI), formula (VII), formula (VIII), formula (IX), formula (X) or formula (XI) according to any one of claims 1-13, or a pharmaceutically acceptable salt, stereoisomer, solvate, polymorph, tautomer, isotopic compound, metabolite or prodrug thereof, and at least one pharmaceutically acceptable carrier.

15. The use of any one of claims 1-14, wherein said PolyQ-related neurodegenerative disease is selected from spinocerebellar ataxia 1, spinocerebellar ataxia 2, spinocerebellar ataxia 3, spinocerebellar ataxia 7, spinocerebellar ataxia 12, spinocerebellar ataxia 17, dentate nucleus-substantia nigra-rednucleus-globus-thalamus atrophy, huntington's disease, and spinobulbar muscular atrophy, in particular huntington's disease and spinocerebellar ataxia 3.

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