CN112755254A - Preparation method of tracheal cannula with antibacterial effect - Google Patents
Preparation method of tracheal cannula with antibacterial effect Download PDFInfo
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- CN112755254A CN112755254A CN202110063433.1A CN202110063433A CN112755254A CN 112755254 A CN112755254 A CN 112755254A CN 202110063433 A CN202110063433 A CN 202110063433A CN 112755254 A CN112755254 A CN 112755254A
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- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/14—Materials characterised by their function or physical properties, e.g. lubricating compositions
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- A61M16/00—Devices for influencing the respiratory system of patients by gas treatment, e.g. mouth-to-mouth respiration; Tracheal tubes
- A61M16/04—Tracheal tubes
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- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
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- D04H3/00—Non-woven fabrics formed wholly or mainly of yarns or like filamentary material of substantial length
- D04H3/02—Non-woven fabrics formed wholly or mainly of yarns or like filamentary material of substantial length characterised by the method of forming fleeces or layers, e.g. reorientation of yarns or filaments
- D04H3/07—Non-woven fabrics formed wholly or mainly of yarns or like filamentary material of substantial length characterised by the method of forming fleeces or layers, e.g. reorientation of yarns or filaments otherwise than in a plane, e.g. in a tubular way
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- A—HUMAN NECESSITIES
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
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Abstract
The invention relates to a preparation method of a tracheal cannula with antibacterial effect, which can effectively solve the problems that the prior art has no antibacterial function or the combination of a coating, an electrostatic spinning membrane and a suture line is not so tight and is easy to fall off, and the technical scheme for solving the problems is that the tracheal cannula is prepared by the following steps: 1) the electrostatic spinning method is used for obtaining a hollow electrostatic spinning hollow tube with antibacterial effect; 2) chopping an electrostatic spinning film with antibacterial effect obtained by an electrostatic spinning method, and placing the chopped electrostatic spinning film serving as a filler into an electrostatic spinning hollow tube; 3) making holes with proper depth on a common trachea cannula; 4) compounding a hollow tube containing filler with a common trachea cannula; the invention can effectively reduce infection easily caused by various factors in the operation process or the operation suture process, has long-acting antibacterial effect, is not easy to fall off, and is innovation on trachea intubation.
Description
Technical Field
The invention relates to medical equipment, in particular to a preparation method of an antibacterial tracheal cannula.
Background
At present, the tracheal cannula used clinically is mainly made of high molecular materials, although the tracheal cannula has good toughness and mechanical strength, the tracheal cannula often does not have an antibacterial function, and infection is easily caused by various factors in the operation process or the operation suture process, so the tracheal cannula with the antibacterial function is particularly important, and the traditional tracheal cannula adopting a coating method or a functional electrostatic spinning membrane modification method is not tight in combination between the coating or the electrostatic spinning membrane and a suture line and is often easy to fall off. Therefore, improvements and innovations in endotracheal intubation are a problem that needs to be addressed at present.
Disclosure of Invention
In view of the above situation, the present invention aims to provide a method for preparing an endotracheal tube with antibacterial effect, which can effectively solve the problem that the endotracheal tube is not provided with antibacterial function or the combination of a coating, an electrostatic spinning membrane and a suture line is not so tight and is easy to fall off in the prior art.
The technical scheme of the invention is that the preparation method of the tracheal cannula with the antibacterial effect comprises the following steps:
1) preparing an electrostatic spinning hollow tube:
the preparation method comprises the following steps of weighing 3-12g of PLA or PLGA material and 0.2-0.4g of norfloxacin or ciprofloxacin lactate in each 100ml of organic solution, pouring the weighed PLA or PLGA, norfloxacin or ciprofloxacin lactate into the mixed organic solvent, stirring for 1.5 hours by shaking until the PLA or PLGA is completely dissolved to obtain a spinning solution, and then feeding the spinning solution into an electrostatic spinning machine, wherein the electro-spraying conditions of the electrostatic spinning machine are as follows: voltage is 20-30kV, receiving distance is 7-12cm, the propelling speed of the injector is 0.5mL/h, the ambient temperature is 23-27 ℃, the humidity is 60-70%, and the hollow tube of PLA or PLGA electrostatic spinning is obtained by adopting a roller with the diameter of 5mm for receiving;
2) preparing an antibacterial electrostatic spinning membrane:
the preparation method comprises the following steps of weighing 3-12g of PLA or PLGA and 0.2-0.4g of norfloxacin or ciprofloxacin lactate in each 100ml of organic solution, then pouring the weighed PLA or PLGA, norfloxacin or ciprofloxacin lactate into the mixed organic solution, stirring for 1.5 hours by shaking until the PLA or PLGA is completely dissolved to obtain a spinning solution, and then feeding the spinning solution into an electrostatic spinning machine, wherein the electro-spinning conditions of the electrostatic spinning machine are as follows: voltage is 20-30kV, receiving distance is 7-12cm, the advancing speed of the injector is 0.5mL/h, the ambient temperature is 23-27 ℃, the humidity is 60-70%, and a flat plate coated with tinfoil paper is used for receiving to obtain a PLA or PLGA antibacterial electrostatic spinning film;
3) preparing a PLA or PLGA electrostatic spinning hollow tube containing filler:
cutting the prepared PLA or PLGA electrostatic spinning hollow tube into sections, cutting the PLA or PLGA antibacterial electrostatic spinning film into small pieces with proper sizes, and filling the small pieces into the PLA or PLGA electrostatic spinning hollow tube to obtain the PLA or PLGA electrostatic spinning hollow tube containing the filler;
4) preparing the tracheal cannula with antibacterial effect: uniformly manufacturing small holes with the diameter of 4-5mm and the depth matched with the PLA or PLGA electrostatic spinning hollow tube containing the filler on the surface of the common trachea cannula, then plugging the manufactured PLA or PLGA electrostatic spinning hollow tube containing the filler into the small holes, and then adopting a thermal processing method to further process and tighten the compounded trachea cannula to obtain the trachea cannula with the antibacterial effect.
The organic solvent is N, N-dimethylformamide, chloroform/acetone mixed solution with the volume ratio of 2:1, and one or more than two of tetrahydrofuran.
The trachea cannula modified by the hollow tube with the special structure obtained by adopting the electrostatic spinning mode can effectively reduce infection easily caused by various factors in the operation process or the operation suture process, improves the combination between the drug-loaded slow-release microsphere interface and the trachea cannula, has long-acting antibacterial effect, is not easy to fall off, has good application prospect, and is an innovation on the trachea cannula.
Drawings
FIG. 1 is a diagram showing the electrospinning morphology of 3% concentration PLA spinning solution according to the present invention.
FIG. 2 is an electrostatic spinning topography of a 6% concentration PLGA spinning solution of the present invention.
FIG. 3 is a 12% concentration PLA spinning solution electrospinning topography of the present invention.
Fig. 4 is a schematic view of an endotracheal tube of the present invention.
Detailed Description
The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples.
Example 1
In specific implementation, the preparation method of the tracheal cannula with the antibacterial effect comprises the following steps:
1) preparing an electrostatic spinning hollow tube:
taking 5mL of electrostatic spinning solution system as an example, 2.5mL of N, N-dimethylformamide, 1.67mL of chloroform and 0.83mL of acetone are accurately measured by a measuring cylinder, 0.3g of PLA material and 0.01g of norfloxacin are measured by an electronic balance, then the weighed PLA and norfloxacin are poured into the mixed chloroform/acetone/N, N-dimethylformamide solution, the mixture is stirred for 1.5 hours by shaking until the PLA is completely dissolved to obtain spinning solution, and then the spinning solution is fed into an electrostatic spinning machine, wherein the electro-spraying conditions of the electrostatic spinning machine are as follows: the voltage is 20kV, the receiving distance is 7cm, the propelling speed of the injector is 0.5mL/h, the ambient temperature is 23 ℃, the humidity is 60%, and the PLA electrostatic spinning hollow tube is obtained by adopting a roller with the diameter of 5mm for receiving;
2) preparing an antibacterial electrostatic spinning membrane:
taking 10mL of electrostatic spinning solution system as an example, 2.5mL of N, N-dimethylformamide, 1.67mL of chloroform and 0.83mL of acetone are accurately measured by a measuring cylinder, 0.3g of PLA material and 0.01g of norfloxacin are measured by an electronic balance, then the weighed PLA and norfloxacin are poured into the mixed chloroform/acetone/N, N-dimethylformamide solution, the mixture is stirred for 1.5 hours by shaking until the PLA is completely dissolved to obtain spinning solution, and then the spinning solution is fed into an electrostatic spinning machine, wherein the electro-spraying conditions of the electrostatic spinning machine are as follows: the voltage is 20kV, the receiving distance is 7cm, the propelling speed of the injector is 0.5mL/h, the ambient temperature is 23 ℃, the humidity is 60%, and a flat plate coated with tinfoil paper is used for receiving to obtain the PLA antibacterial electrostatic spinning film;
3) preparation of PLA electrospun hollow tube containing filler:
cutting the prepared PLA electrostatic spinning hollow tube into sections, cutting the PLA antibacterial electrostatic spinning film into small blocks with proper size, and filling the small blocks into the PLA electrostatic spinning hollow tube to obtain the PLA electrostatic spinning hollow tube containing the filler;
4) preparing the tracheal cannula with antibacterial effect: uniformly manufacturing small holes with the diameter of 4-5mm and the depth matched with the PLA electrostatic spinning hollow tube containing the filler on the surface of the common trachea cannula, then plugging the manufactured PLA electrostatic spinning hollow tube containing the filler into the small holes, and then adopting a hot processing method to further process and tighten the combined trachea cannula to obtain the trachea cannula with the antibacterial effect.
Example 2
In specific implementation, the preparation method of the tracheal cannula with the antibacterial effect comprises the following steps:
1) preparing an electrostatic spinning hollow tube:
taking 10mL of electrostatic spinning solution system as an example, a measuring cylinder accurately measures 5mL of N, N-dimethylformamide, 3.33mL of chloroform and 1.67mL of acetone, an electronic balance measures 1g of PLGA material and 0.03g of ciprofloxacin lactate, then the weighed PLGA and ciprofloxacin lactate are poured into the mixed chloroform/acetone/N and N-dimethylformamide solution, the mixture is stirred for 1.5 hours by oscillation until the PLGA is completely dissolved to obtain a spinning solution, then the spinning solution is fed into an electrostatic spinning machine, and the electrostatic spinning machine has the following electric spraying conditions: the voltage is 25kV, the receiving distance is 10cm, the propelling speed of the injector is 0.5mL/h, the ambient temperature is 25 ℃, the humidity is 65%, and the PLGA electrostatic spinning hollow tube is obtained by adopting a roller with the diameter of 5mm for receiving;
2) preparing an antibacterial electrostatic spinning membrane:
taking 10mL of electrostatic spinning solution system as an example, a measuring cylinder accurately measures 5mL of N, N-dimethylformamide, 3.33mL of chloroform and 1.67mL of acetone, an electronic balance measures 1g of PLGA material and 0.03g of ciprofloxacin lactate, then the weighed PLGA and ciprofloxacin lactate are poured into the mixed chloroform/acetone/N and N-dimethylformamide solution, the mixture is stirred for 1.5 hours by oscillation until the PLGA is completely dissolved to obtain a spinning solution, then the spinning solution is fed into an electrostatic spinning machine, and the electrostatic spinning machine has the following electric spraying conditions: the voltage is 25kV, the receiving distance is 10cm, the propelling speed of the injector is 0.5mL/h, the ambient temperature is 25 ℃, the humidity is 65%, and the PLGA antibacterial electrostatic spinning film is obtained by adopting a flat plate coated with tinfoil paper for receiving;
3) preparing a PLGA electrostatic spinning hollow tube containing filler:
cutting the prepared PLGA electrostatic spinning hollow tube into sections, cutting the PLGA antibacterial electrostatic spinning film into small pieces with proper size, and filling the small pieces into the PLGA electrostatic spinning hollow tube to obtain a PLGA electrostatic spinning hollow tube containing fillers;
4) preparing the tracheal cannula with antibacterial effect: uniformly manufacturing small holes with the diameter of 4-5mm and the depth matched with the PLGA electrostatic spinning hollow tube containing the filler on the surface of the common trachea cannula, then plugging the manufactured PLGA electrostatic spinning hollow tube containing the filler into the small holes, and then adopting a hot processing method to further process and tighten the compounded trachea cannula to obtain the trachea cannula with the antibacterial effect.
Example 3
In specific implementation, the preparation method of the tracheal cannula with the antibacterial effect comprises the following steps:
1) preparing an electrostatic spinning hollow tube:
taking 15mL of electrostatic spinning solution system as an example, 7.5mL of N, N-dimethylformamide, 5mL of chloroform and 2.5mL of acetone are accurately measured by a measuring cylinder, 1.8g of PLA material and 0.06g of norfloxacin are weighed by an electronic balance, then the weighed PLA and norfloxacin are poured into the mixed chloroform/acetone/N, N-dimethylformamide solution, the mixture is stirred for 1.5 hours by shaking until the PLA is completely dissolved to obtain spinning solution, then the spinning solution is fed into an electrostatic spinning machine, and the electro-spraying conditions of the electrostatic spinning machine are as follows: the voltage is 30kV, the receiving distance is 12cm, the propelling speed of the injector is 0.5mL/h, the ambient temperature is 27 ℃, the humidity is 70%, and the PLA electrostatic spinning hollow tube is obtained by adopting a roller with the diameter of 5mm for receiving;
2) preparing an antibacterial electrostatic spinning membrane:
taking 15mL of electrostatic spinning solution system as an example, 7.5mL of N, N-dimethylformamide, 5mL of chloroform and 2.5mL of acetone are accurately measured by a measuring cylinder, 1.8g of PLA material and 0.06g of norfloxacin are weighed by an electronic balance, then the weighed PLA and norfloxacin are poured into the mixed chloroform/acetone/N, N-dimethylformamide solution, the mixture is stirred for 1.5 hours by shaking until the PLA is completely dissolved to obtain spinning solution, then the spinning solution is fed into an electrostatic spinning machine, and the electro-spraying conditions of the electrostatic spinning machine are as follows: the voltage is 30kV, the receiving distance is 12cm, the propelling speed of the injector is 0.5mL/h, the ambient temperature is 27 ℃, the humidity is 70%, and a flat plate coated with tinfoil paper is used for receiving to obtain the PLA antibacterial electrostatic spinning film;
3) preparation of PLA electrospun hollow tube containing filler:
cutting the prepared PLA electrostatic spinning hollow tube into sections, cutting the PLA antibacterial electrostatic spinning film into small blocks with proper size, and filling the small blocks into the PLA electrostatic spinning hollow tube to obtain the PLA electrostatic spinning hollow tube containing the filler;
4) preparing the tracheal cannula with antibacterial effect: uniformly manufacturing small holes with the diameter of 4-5mm and the depth matched with the PLA electrostatic spinning hollow tube containing the filler on the surface of the common trachea cannula, then plugging the manufactured PLA electrostatic spinning hollow tube containing the filler into the small holes, and then adopting a hot processing method to further process and tighten the combined trachea cannula to obtain the trachea cannula with the antibacterial effect.
The electrostatic spinning hollow tube prepared by the invention has a special structure, is fixed on the surface of a suture line in an embedding mode, is not easy to fall off, has a longer application effect in the aspect of medicine application, can continuously release for more than 14 days (electrostatic spinning topography graphs with different concentrations are shown in figures 1, 2 and 3, and a prepared trachea cannula is shown in figure 4), and is fully proved by experiments, and relevant test data are as follows:
the PLA microsphere antibacterial experiment of the encapsulated norfloxacin with antibacterial effect comprises the following steps:
(1) preparing and sterilizing an agar culture medium;
(2) preparation of bacterial suspension: in clean benches, with sterilityScraping Staphylococcus aureus and Escherichia coli colonies with cotton swab to sterile sodium chloride-peptone solution with pH of 7.0, and adding 6 × 108comparing cfu/mL turbidimetric tubes, performing gradient dilution after the concentration is reached, and obtaining the final bacterial liquid concentration of 6 x 105cfu/mL, followed by 6 x 10 dip with sterile cotton swab5cfu/mL solution, in pancreas casein soya peptone agar medium culture dish evenly spread.
(3) Pasting and placing the bacteriostatic sample: a sample piece with the diameter area of 5mm is cut from the prepared electrostatic spinning membrane, the sample piece is lightly pressed into a culture dish coated with bacteria, the culture dish is covered, and the sample piece is placed in an incubator to be cultured for 16-18 h.
(4) And (3) judging the bacteriostatic action: the antibacterial ring is larger than 7mm, and has antibacterial effect.
Table 1 shows the bacteriostatic effect of PLA with different proportional concentrations
Through repeated experiments, other embodiments of the present invention can achieve the same or similar effect as the above experimental data, and are not described in detail.
Compared with the prior art, the invention has the following advantages:
1. the invention adopts environment-friendly and pollution-free PLA or PLGA material as the medicine carrying material, and has the characteristic of good biocompatibility.
2. The hollow tube manufactured by the invention can adjust the form of the microsphere nanofiber according to different formulas and change the basic characteristics of the interface, thereby improving the application range of the microsphere interface and having good drug loading effect.
3. The hollow tube prepared by the invention is coated with norfloxacin or ciprofloxacin lactate with antibacterial effect, and has good practical value in the field of biological medicine.
4. The hollow tube manufactured by the invention is skillfully embedded on the surface of the tracheal cannula, so that the friction and falling caused by inserting the hollow tube into an airway can be reduced to a great extent, and the effectiveness and durability of antibiosis are improved.
5. The electrostatic spinning hollow tube obtained by the invention has a special structure, is fixed on the surface of a suture line in an embedding mode, is not easy to fall off, has a longer application effect in the aspect of medicine application, and can continuously release for more than 14 days.
Claims (4)
1. A preparation method of a tracheal cannula with antibacterial effect is characterized by comprising the following steps:
1) preparing an electrostatic spinning hollow tube:
the preparation method comprises the following steps of weighing 3-12g of PLA or PLGA material and 0.2-0.4g of norfloxacin or ciprofloxacin lactate in each 100ml of organic solution, pouring the weighed PLA or PLGA, norfloxacin or ciprofloxacin lactate into the mixed organic solvent, stirring for 1.5 hours by shaking until the PLA or PLGA is completely dissolved to obtain a spinning solution, and then feeding the spinning solution into an electrostatic spinning machine, wherein the electro-spraying conditions of the electrostatic spinning machine are as follows: voltage is 20-30kV, receiving distance is 7-12cm, the propelling speed of the injector is 0.5mL/h, the ambient temperature is 23-27 ℃, the humidity is 60-70%, and the hollow tube of PLA or PLGA electrostatic spinning is obtained by adopting a roller with the diameter of 5mm for receiving;
2) preparing an antibacterial electrostatic spinning membrane:
the preparation method comprises the following steps of weighing 3-12g of PLA or PLGA and 0.2-0.4g of norfloxacin or ciprofloxacin lactate in each 100ml of organic solution, then pouring the weighed PLA or PLGA, norfloxacin or ciprofloxacin lactate into the mixed organic solution, stirring for 1.5 hours by shaking until the PLA or PLGA is completely dissolved to obtain a spinning solution, and then feeding the spinning solution into an electrostatic spinning machine, wherein the electro-spinning conditions of the electrostatic spinning machine are as follows: voltage is 20-30kV, receiving distance is 7-12cm, the advancing speed of the injector is 0.5mL/h, the ambient temperature is 23-27 ℃, the humidity is 60-70%, and a flat plate coated with tinfoil paper is used for receiving to obtain a PLA or PLGA antibacterial electrostatic spinning film;
3) preparing a PLA or PLGA electrostatic spinning hollow tube containing filler:
cutting the prepared PLA or PLGA electrostatic spinning hollow tube into sections, cutting the PLA or PLGA antibacterial electrostatic spinning film into small pieces with proper sizes, and filling the small pieces into the PLA or PLGA electrostatic spinning hollow tube to obtain the PLA or PLGA electrostatic spinning hollow tube containing the filler;
4) preparing the tracheal cannula with antibacterial effect: uniformly manufacturing small holes with the diameter of 4-5mm and the depth matched with the PLA or PLGA electrostatic spinning hollow tube containing the filler on the surface of the common trachea cannula, then plugging the manufactured PLA or PLGA electrostatic spinning hollow tube containing the filler into the small holes, and then further processing and fastening the combined trachea cannula by adopting a hot processing method to obtain the trachea cannula with an antibacterial effect;
the organic solvent is N, N-dimethylformamide, chloroform/acetone mixed solution with the volume ratio of 2:1, and one or more than two of tetrahydrofuran.
2. The method for preparing an endotracheal tube having an antibacterial effect according to claim 1, characterized by comprising the steps of:
1) preparing an electrostatic spinning hollow tube:
taking 5mL of electrostatic spinning solution system as an example, 2.5mL of N, N-dimethylformamide, 1.67mL of chloroform and 0.83mL of acetone are accurately measured by a measuring cylinder, 0.3g of PLA material and 0.01g of norfloxacin are measured by an electronic balance, then the weighed PLA and norfloxacin are poured into the mixed chloroform/acetone/N, N-dimethylformamide solution, the mixture is stirred for 1.5 hours by shaking until the PLA is completely dissolved to obtain spinning solution, and then the spinning solution is fed into an electrostatic spinning machine, wherein the electro-spraying conditions of the electrostatic spinning machine are as follows: the voltage is 20kV, the receiving distance is 7cm, the propelling speed of the injector is 0.5mL/h, the ambient temperature is 23 ℃, the humidity is 60%, and the PLA electrostatic spinning hollow tube is obtained by adopting a roller with the diameter of 5mm for receiving;
2) preparing an antibacterial electrostatic spinning membrane:
taking 10mL of electrostatic spinning solution system as an example, 2.5mL of N, N-dimethylformamide, 1.67mL of chloroform and 0.83mL of acetone are accurately measured by a measuring cylinder, 0.3g of PLA material and 0.01g of norfloxacin are measured by an electronic balance, then the weighed PLA and norfloxacin are poured into the mixed chloroform/acetone/N, N-dimethylformamide solution, the mixture is stirred for 1.5 hours by shaking until the PLA is completely dissolved to obtain spinning solution, and then the spinning solution is fed into an electrostatic spinning machine, wherein the electro-spraying conditions of the electrostatic spinning machine are as follows: the voltage is 20kV, the receiving distance is 7cm, the propelling speed of the injector is 0.5mL/h, the ambient temperature is 23 ℃, the humidity is 60%, and a flat plate coated with tinfoil paper is used for receiving to obtain the PLA antibacterial electrostatic spinning film;
3) preparation of PLA electrospun hollow tube containing filler:
cutting the prepared PLA electrostatic spinning hollow tube into sections, cutting the PLA antibacterial electrostatic spinning film into small blocks with proper size, and filling the small blocks into the PLA electrostatic spinning hollow tube to obtain the PLA electrostatic spinning hollow tube containing the filler;
4) preparing the tracheal cannula with antibacterial effect: uniformly manufacturing small holes with the diameter of 4-5mm and the depth matched with the PLA electrostatic spinning hollow tube containing the filler on the surface of the common trachea cannula, then plugging the manufactured PLA electrostatic spinning hollow tube containing the filler into the small holes, and then adopting a hot processing method to further process and tighten the combined trachea cannula to obtain the trachea cannula with the antibacterial effect.
3. The method for preparing an endotracheal tube having an antibacterial effect according to claim 1, characterized by comprising the steps of:
1) preparing an electrostatic spinning hollow tube:
taking 10mL of electrostatic spinning solution system as an example, a measuring cylinder accurately measures 5mL of N, N-dimethylformamide, 3.33mL of chloroform and 1.67mL of acetone, an electronic balance measures 1g of PLGA material and 0.03g of ciprofloxacin lactate, then the weighed PLGA and ciprofloxacin lactate are poured into the mixed chloroform/acetone/N and N-dimethylformamide solution, the mixture is stirred for 1.5 hours by oscillation until the PLGA is completely dissolved to obtain a spinning solution, then the spinning solution is fed into an electrostatic spinning machine, and the electrostatic spinning machine has the following electric spraying conditions: the voltage is 25kV, the receiving distance is 10cm, the propelling speed of the injector is 0.5mL/h, the ambient temperature is 25 ℃, the humidity is 65%, and the PLGA electrostatic spinning hollow tube is obtained by adopting a roller with the diameter of 5mm for receiving;
2) preparing an antibacterial electrostatic spinning membrane:
taking 10mL of electrostatic spinning solution system as an example, a measuring cylinder accurately measures 5mL of N, N-dimethylformamide, 3.33mL of chloroform and 1.67mL of acetone, an electronic balance measures 1g of PLGA material and 0.03g of ciprofloxacin lactate, then the weighed PLGA and ciprofloxacin lactate are poured into the mixed chloroform/acetone/N and N-dimethylformamide solution, the mixture is stirred for 1.5 hours by oscillation until the PLGA is completely dissolved to obtain a spinning solution, then the spinning solution is fed into an electrostatic spinning machine, and the electrostatic spinning machine has the following electric spraying conditions: the voltage is 25kV, the receiving distance is 10cm, the propelling speed of the injector is 0.5mL/h, the ambient temperature is 25 ℃, the humidity is 65%, and the PLGA antibacterial electrostatic spinning film is obtained by adopting a flat plate coated with tinfoil paper for receiving;
3) preparing a PLGA electrostatic spinning hollow tube containing filler:
cutting the prepared PLGA electrostatic spinning hollow tube into sections, cutting the PLGA antibacterial electrostatic spinning film into small pieces with proper size, and filling the small pieces into the PLGA electrostatic spinning hollow tube to obtain a PLGA electrostatic spinning hollow tube containing fillers;
4) preparing the tracheal cannula with antibacterial effect: uniformly manufacturing small holes with the diameter of 4-5mm and the depth matched with the PLGA electrostatic spinning hollow tube containing the filler on the surface of the common trachea cannula, then plugging the manufactured PLGA electrostatic spinning hollow tube containing the filler into the small holes, and then adopting a hot processing method to further process and tighten the compounded trachea cannula to obtain the trachea cannula with the antibacterial effect.
4. The method for preparing an endotracheal tube having an antibacterial effect according to claim 1, characterized by comprising the steps of:
1) preparing an electrostatic spinning hollow tube:
taking 15mL of electrostatic spinning solution system as an example, 7.5mL of N, N-dimethylformamide, 5mL of chloroform and 2.5mL of acetone are accurately measured by a measuring cylinder, 1.8g of PLA material and 0.06g of norfloxacin are weighed by an electronic balance, then the weighed PLA and norfloxacin are poured into the mixed chloroform/acetone/N, N-dimethylformamide solution, the mixture is stirred for 1.5 hours by shaking until the PLA is completely dissolved to obtain spinning solution, then the spinning solution is fed into an electrostatic spinning machine, and the electro-spraying conditions of the electrostatic spinning machine are as follows: the voltage is 30kV, the receiving distance is 12cm, the propelling speed of the injector is 0.5mL/h, the ambient temperature is 27 ℃, the humidity is 70%, and the PLA electrostatic spinning hollow tube is obtained by adopting a roller with the diameter of 5mm for receiving;
2) preparing an antibacterial electrostatic spinning membrane:
taking 15mL of electrostatic spinning solution system as an example, 7.5mL of N, N-dimethylformamide, 5mL of chloroform and 2.5mL of acetone are accurately measured by a measuring cylinder, 1.8g of PLA material and 0.06g of norfloxacin are weighed by an electronic balance, then the weighed PLA and norfloxacin are poured into the mixed chloroform/acetone/N, N-dimethylformamide solution, the mixture is stirred for 1.5 hours by shaking until the PLA is completely dissolved to obtain spinning solution, then the spinning solution is fed into an electrostatic spinning machine, and the electro-spraying conditions of the electrostatic spinning machine are as follows: the voltage is 30kV, the receiving distance is 12cm, the propelling speed of the injector is 0.5mL/h, the ambient temperature is 27 ℃, the humidity is 70%, and a flat plate coated with tinfoil paper is used for receiving to obtain the PLA antibacterial electrostatic spinning film;
3) preparation of PLA electrospun hollow tube containing filler:
cutting the prepared PLA electrostatic spinning hollow tube into sections, cutting the PLA antibacterial electrostatic spinning film into small blocks with proper size, and filling the small blocks into the PLA electrostatic spinning hollow tube to obtain the PLA electrostatic spinning hollow tube containing the filler;
4) preparing the tracheal cannula with antibacterial effect: uniformly manufacturing small holes with the diameter of 4-5mm and the depth matched with the PLA electrostatic spinning hollow tube containing the filler on the surface of the common trachea cannula, then plugging the manufactured PLA electrostatic spinning hollow tube containing the filler into the small holes, and then adopting a hot processing method to further process and tighten the combined trachea cannula to obtain the trachea cannula with the antibacterial effect.
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