CN112730669A - Method for measuring cinnamaldehyde, eugenol, carvacrol and thymol - Google Patents
Method for measuring cinnamaldehyde, eugenol, carvacrol and thymol Download PDFInfo
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- CN112730669A CN112730669A CN202011535938.5A CN202011535938A CN112730669A CN 112730669 A CN112730669 A CN 112730669A CN 202011535938 A CN202011535938 A CN 202011535938A CN 112730669 A CN112730669 A CN 112730669A
- Authority
- CN
- China
- Prior art keywords
- solution
- measuring
- carvacrol
- eugenol
- cinnamaldehyde
- Prior art date
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- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 title claims abstract description 36
- MGSRCZKZVOBKFT-UHFFFAOYSA-N thymol Chemical compound CC(C)C1=CC=C(C)C=C1O MGSRCZKZVOBKFT-UHFFFAOYSA-N 0.000 title claims abstract description 36
- KJPRLNWUNMBNBZ-QPJJXVBHSA-N (E)-cinnamaldehyde Chemical compound O=C\C=C\C1=CC=CC=C1 KJPRLNWUNMBNBZ-QPJJXVBHSA-N 0.000 title claims abstract description 18
- NPBVQXIMTZKSBA-UHFFFAOYSA-N Chavibetol Natural products COC1=CC=C(CC=C)C=C1O NPBVQXIMTZKSBA-UHFFFAOYSA-N 0.000 title claims abstract description 18
- 239000005770 Eugenol Substances 0.000 title claims abstract description 18
- UVMRYBDEERADNV-UHFFFAOYSA-N Pseudoeugenol Natural products COC1=CC(C(C)=C)=CC=C1O UVMRYBDEERADNV-UHFFFAOYSA-N 0.000 title claims abstract description 18
- 239000005844 Thymol Substances 0.000 title claims abstract description 18
- RECUKUPTGUEGMW-UHFFFAOYSA-N carvacrol Chemical compound CC(C)C1=CC=C(C)C(O)=C1 RECUKUPTGUEGMW-UHFFFAOYSA-N 0.000 title claims abstract description 18
- HHTWOMMSBMNRKP-UHFFFAOYSA-N carvacrol Natural products CC(=C)C1=CC=C(C)C(O)=C1 HHTWOMMSBMNRKP-UHFFFAOYSA-N 0.000 title claims abstract description 18
- 235000007746 carvacrol Nutrition 0.000 title claims abstract description 18
- 229960002217 eugenol Drugs 0.000 title claims abstract description 18
- WYXXLXHHWYNKJF-UHFFFAOYSA-N isocarvacrol Natural products CC(C)C1=CC=C(O)C(C)=C1 WYXXLXHHWYNKJF-UHFFFAOYSA-N 0.000 title claims abstract description 18
- 229960000790 thymol Drugs 0.000 title claims abstract description 18
- 229940117916 cinnamic aldehyde Drugs 0.000 title claims abstract description 17
- KJPRLNWUNMBNBZ-UHFFFAOYSA-N cinnamic aldehyde Natural products O=CC=CC1=CC=CC=C1 KJPRLNWUNMBNBZ-UHFFFAOYSA-N 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 15
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000000243 solution Substances 0.000 claims abstract description 21
- 239000000523 sample Substances 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 238000005259 measurement Methods 0.000 claims abstract description 9
- 238000004811 liquid chromatography Methods 0.000 claims abstract description 8
- 239000012528 membrane Substances 0.000 claims abstract description 8
- 239000012488 sample solution Substances 0.000 claims abstract description 8
- 238000001514 detection method Methods 0.000 claims abstract description 7
- 239000008367 deionised water Substances 0.000 claims abstract description 4
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 4
- 238000007865 diluting Methods 0.000 claims abstract description 4
- 238000010790 dilution Methods 0.000 claims abstract description 4
- 239000012895 dilution Substances 0.000 claims abstract description 4
- 238000010812 external standard method Methods 0.000 claims abstract description 4
- 238000012360 testing method Methods 0.000 claims abstract description 4
- 238000009210 therapy by ultrasound Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000005303 weighing Methods 0.000 claims abstract description 4
- 239000012224 working solution Substances 0.000 claims abstract description 4
- 238000001914 filtration Methods 0.000 claims abstract description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 9
- 239000002245 particle Substances 0.000 claims description 3
- 239000000341 volatile oil Substances 0.000 abstract description 6
- 230000004075 alteration Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/30—Control of physical parameters of the fluid carrier of temperature
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
- G01N2030/324—Control of physical parameters of the fluid carrier of pressure or speed speed, flow rate
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention discloses a method for measuring cinnamaldehyde, eugenol, carvacrol and thymol, belonging to the field of detection, and the method for measuring cinnamaldehyde, eugenol, carvacrol and thymol comprises the following steps: a. preparing a measuring solution; weighing 0.5g-1g (accurate to 0.0001g) of sample in a 50mL volumetric flask, adding 30mL of methanol, performing ultrasonic treatment for 20-30min, and fixing the volume of the methanol to the scale of the volumetric flask; b. solution dilution: diluting the prepared solution with deionized water; c. filtering the solution: passing the diluted solution through a filter membrane to obtain a test sample solution; d. liquid chromatography determination: injecting the prepared sample solution and the standard working solution into a liquid chromatograph for measurement; e. content determination: calculating the content of effective components in the sample by using a peak height external standard method; the invention can directly extract four different components in the plant essential oil, directly measure by a liquid chromatograph, and has simple and rapid operation and high efficiency.
Description
Technical Field
The invention belongs to the technical field of detection, and particularly relates to a determination method of cinnamaldehyde, eugenol, carvacrol and thymol.
Background
Plant essential oil is widely used in the feed industry as one of the substitute antibiotics in the animal husbandry at present. The plant essential oil has good antibacterial activity, palatability and growth promoting effect, and can be used as a good antibiotic substitute. Cinnamaldehyde, eugenol, carvacrol and thymol are used as a plurality of commonly used plant essential oil components, most of the detection methods are gas chromatography, and liquid chromatography is rarely used, or one or two components are detected by the liquid chromatography, and no four components are detected by the liquid chromatography together temporarily. The invention aims to solve the problem of liquid chromatography detection of four components.
Disclosure of Invention
The invention aims to provide a method for measuring cinnamaldehyde, eugenol, carvacrol and thymol.
In order to achieve the purpose, the invention provides the following technical scheme: a method for measuring cinnamaldehyde, eugenol, carvacrol and thymol comprises the following steps:
a. preparing a measuring solution; weighing 0.5g-1g (accurate to 0.0001g) of sample in a 50mL volumetric flask, adding 30mL of methanol, performing ultrasonic treatment for 20-30min, and fixing the volume of the methanol to the scale of the volumetric flask;
b. solution dilution: diluting the prepared solution with deionized water;
c. filtering the solution: passing the diluted solution through a filter membrane to obtain a test sample solution;
d. liquid chromatography determination: injecting the prepared sample solution and the standard working solution into a liquid chromatograph for measurement;
e. content determination: and calculating the content of the effective components in the sample by using a peak height external standard method.
Preferably, when the solution is diluted, the solution is diluted to the concentration of the standard curve of the four components of the cinnamaldehyde, the eugenol, the carvacrol and the thymol, wherein the lowest standard concentration range.
Preferably, the filter membrane is a 0.45 μm size filter membrane.
Preferably, when the measurement is carried out by a liquid chromatograph, the measurement is carried out according to the following chromatographic conditions:
a chromatographic column: c18 column, 150mm × 4.6mm, particle size 5 μm.
Mobile phase: acetonitrile + water-40 + 60.
Detection wavelength: 280 nm.
Column temperature: at 40 ℃.
Flow rate: 1.0 ml/min.
Sample introduction amount: 20 μ L.
The invention has the beneficial effects that:
1. the liquid chromatograph is used for detecting the four components of the cinnamyl aldehyde, the eugenol, the carvacrol and the thymol in the plant essential oil, and the liquid chromatograph is used as a stock instrument in the feed industry, so that the use cost is low, the four different components in the plant essential oil are directly extracted, the measurement is directly carried out by the liquid chromatograph, the operation is simple and rapid, and the efficiency is high.
2. The method has simple sample pretreatment process and greatly improves the operation efficiency.
Detailed Description
In order to further understand the contents, features and effects of the present invention, the following examples are given.
The measuring methods of cinnamaldehyde, eugenol, carvacrol and thymol according to the examples of the present invention will be described in detail below.
The method for measuring the cinnamaldehyde, the eugenol, the carvacrol and the thymol comprises the following steps:
sample processing
Weighing 0.5g-1g (accurate to 0.0001g) sample in a 50mL volumetric flask, adding 30mL methanol, performing ultrasonic treatment for 20-30min, and metering the volume of the methanol to the scale of the volumetric flask.
Measurement sample preparation
Diluting the prepared solution with deionized water to the concentration of standard curves of the four components of cinnamaldehyde, eugenol, carvacrol and thymol, wherein the concentration is within the lowest standard concentration range; the diluted solution was passed through a filter membrane of 0.45 μm size to obtain a test sample solution.
Liquid chromatography assay
The following chromatographic conditions were used:
a chromatographic column: c18 column, 150mm × 4.6mm, particle size 5 μm.
Mobile phase: acetonitrile + water-40 + 60.
Detection wavelength: 280 nm.
Column temperature: at 40 ℃.
Flow rate: 1.0 ml/min.
Sample introduction amount: 20 mu L of the solution;
and injecting the prepared sample solution and the standard working solution into a liquid chromatograph for measurement.
Determination of component content
And calculating the content of the effective components in the sample by using a peak height external standard method.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (4)
1. A method for measuring cinnamaldehyde, eugenol, carvacrol and thymol is characterized by comprising the following steps:
a. preparing a measuring solution; weighing 0.5g-1g (accurate to 0.0001g) of sample in a 50mL volumetric flask, adding 30mL of methanol, performing ultrasonic treatment for 20-30min, and fixing the volume of the methanol to the scale of the volumetric flask;
b. solution dilution: diluting the prepared solution with deionized water;
c. filtering the solution: passing the diluted solution through a filter membrane to obtain a test sample solution;
d. liquid chromatography determination: injecting the prepared sample solution and the standard working solution into a liquid chromatograph for measurement;
e. content determination: and calculating the content of the effective components in the sample by using a peak height external standard method.
2. The method for measuring cinnamaldehyde, eugenol, carvacrol and thymol according to claim 1, wherein the dilution of the solution is performed to a concentration within a standard curve of four components of cinnamaldehyde, eugenol, carvacrol and thymol, wherein the concentration is within the lowest standard concentration range.
3. The method for measuring cinnamaldehyde, eugenol, carvacrol and thymol according to claim 1, wherein the filter membrane is a 0.45 μm size filter membrane.
4. The method for measuring cinnamaldehyde, eugenol, carvacrol and thymol according to claim 1, wherein the measurement is performed by a liquid chromatograph under the following chromatographic conditions:
a chromatographic column: c18 column, 150mm × 4.6mm, particle size 5 μm.
Mobile phase: acetonitrile + water-40 + 60.
Detection wavelength: 280 nm.
Column temperature: at 40 ℃.
Flow rate: 1.0 ml/min.
Sample introduction amount: 20 μ L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011535938.5A CN112730669A (en) | 2020-12-23 | 2020-12-23 | Method for measuring cinnamaldehyde, eugenol, carvacrol and thymol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011535938.5A CN112730669A (en) | 2020-12-23 | 2020-12-23 | Method for measuring cinnamaldehyde, eugenol, carvacrol and thymol |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112730669A true CN112730669A (en) | 2021-04-30 |
Family
ID=75604771
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011535938.5A Pending CN112730669A (en) | 2020-12-23 | 2020-12-23 | Method for measuring cinnamaldehyde, eugenol, carvacrol and thymol |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112730669A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113777196A (en) * | 2021-09-13 | 2021-12-10 | 天津春发生物科技集团有限公司 | Method for simultaneously determining six spicy components in solid food by DAD-HPLC |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020107287A1 (en) * | 1999-04-29 | 2002-08-08 | Bessette Steven M. | Pesticidal activity of plant essential oils and their constituents |
| CN102014616A (en) * | 2008-03-19 | 2011-04-13 | 蒂拉德克公司 | Pest control using natural pest control agent blends |
| CN107843676A (en) * | 2017-11-15 | 2018-03-27 | 广州美瑞泰科生物工程技术有限公司 | The detection method of carvacrol and Thymol in a kind of plant extract feed additive |
-
2020
- 2020-12-23 CN CN202011535938.5A patent/CN112730669A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020107287A1 (en) * | 1999-04-29 | 2002-08-08 | Bessette Steven M. | Pesticidal activity of plant essential oils and their constituents |
| CN102014616A (en) * | 2008-03-19 | 2011-04-13 | 蒂拉德克公司 | Pest control using natural pest control agent blends |
| CN107843676A (en) * | 2017-11-15 | 2018-03-27 | 广州美瑞泰科生物工程技术有限公司 | The detection method of carvacrol and Thymol in a kind of plant extract feed additive |
Non-Patent Citations (2)
| Title |
|---|
| 任玉琴 等: "高效液相色谱法同时测定饲料香味剂中肉桂醛、丁香酚、香芹酚和百里香酚含量的研究", 《中国饲料》 * |
| 张玮 等: "高效液相色谱法测定混合型饲料添加剂中肉桂醛、丁香酚、香芹酚和百里香酚含量", 《饲料研究》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113777196A (en) * | 2021-09-13 | 2021-12-10 | 天津春发生物科技集团有限公司 | Method for simultaneously determining six spicy components in solid food by DAD-HPLC |
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Application publication date: 20210430 |
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