CN112730576A - Method for detecting stable isotope of garlic sulfur in tissue culture of rachis - Google Patents
Method for detecting stable isotope of garlic sulfur in tissue culture of rachis Download PDFInfo
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Abstract
The invention discloses a method for using SO+The method for detecting the stable isotope of the sulfur in the rachis tissue culture garlic by the mode comprises the specific steps of drying, grinding and filtering a rachis tissue culture garlic bulb sample by a screen, then filling the sample into a tin cup and wrapping the sample, and detecting the sample to be detected by adopting an element analyzer-stable isotope mass spectrometer. The invention utilizes ion source high-energy electron current to SO2 +By tuning the voltage-carrier gas pressure of the quadrupole mass analyzer to produce SO+And efficient separation and accurate detection are carried out, so that the aims of efficient and accurate detection of the sulfur isotope ratio of the garlic in the rachis tissue culture are fulfilled, the result precision is high, the repeatability is good, and the efficiency is high.
Description
The invention obtains the conversion and promotion project subsidized by the agricultural achievement of Tianjin City farming committee in 2021 years.
Technical Field
The invention belongs to the technical field of stable isotope analysis, and particularly relates to a garlic sulfur stable isotope detection method.
Background
The garlic is an important spicy vegetable variety on the dining table of people in China, and has important edible and medicinal values. Various researches have proved that various bioactive substances contained in garlic, such as garlicin, polysaccharide and the like, have more remarkable effects of resisting virus, inhibiting cancer cell activity, resisting thrombus, reducing blood fat, reducing blood pressure, relieving atherosclerosis and the like.
The plant tissue culture refers to a biological technology for culturing isolated plant organs, tissues, cells or protoplasts by artificially controlling conditions such as temperature, illumination, humidity, nutrition, hormone and the like under the sterile environment condition and utilizing a culture medium to provide nutrients so as to regenerate the cells or form a complete plant. The material is garlic obtained by taking the rachis of a special Diwu-Diwu garlic variety 'hexapetalous red' with excellent agronomic characters as an explant to induce adventitious buds and then inducing the bud into a test tube bulb to carry out field cultivation. How to evaluate the stability of the rachis tissue culture plants to obtain the good agronomic characters of the parent plants and comprehensively evaluate and control the quality of the callus tissue culture plants is a problem to be solved urgently in the production aspect of the field.
With the development and maturity of sulfur stable isotope analysis technology, the application of the sulfur stable isotope analysis technology in the aspects of environmental protection, agricultural production and the like is concerned widely. Currently, reports for researching sulfur element source transformation mechanism and bioavailability in plants by using a sulfur stable isotope technology are frequent.
At present, the common sulfur stable isotope analysis method is that an element analyzer is used in combination with a mass spectrometer, namely, sulfur in a sample is oxidized into sulfur dioxide by the element analyzer, and then the sulfur dioxide (SO) is oxidized by an ion source of the isotope mass spectrometer2) Conversion to sulfur dioxide ion (SO)2 +) Sulfur isotope ratio analysis was performed. But SO involved in the process2 +(and its δ S34 isotope) have a high mass-to-charge ratio of 64, 66 m/e. The stable isotope mass spectrometer generally simultaneously undertakes the carbon-nitrogen isotope detection task, and the mass-to-charge ratios of the carbon dioxide (and the delta C13 isotope thereof) and the nitrogen ion (and the delta N15 isotope thereof) involved in the stable isotope mass spectrometer are respectively CO2 +(m/e 44, 45) and N2 +(m/e 28, 29). Due to SO in conventional sulfur dioxide analysis2 +The mass-to-charge ratio is obviously higher than that of CO2 +And N2 +Mass to charge ratio. When the carbon-nitrogen isotope detection is converted into the sulfur isotope detection, the SO with higher mass-to-charge ratio is influenced by the instrument memory effect2 +It is difficult to focus on the receiver effectively, affecting the detection result. To eliminate the memory effect of the instrument, long-time debugging work is often required to improve the accuracy and reproducibility of sulfur isotope detection, and vice versa.
The invention utilizes ion source high-energy electron current to SO2 +Ion secondary ionization technique to generate sulfur monoxide ion (SO)+) By tuning the quadrupole mass analyser voltage and controlling the carrier gas pressure to SO+And carrying out efficient separation and accurate detection on the ion beam. SO (SO)+And δ S34 isotope mass-to-charge ratio of 48, 50, and CO2 +And N2 +The mass-to-charge ratio is closer, which is beneficial to improving the accuracy and the reproducibility of the detection of the sulfur stable isotope, reducing the influence of the memory effect and improving the detection efficiency.
The invention utilizes ion source high-energy electron current to SO2 +Secondary ionization of ions to produce SO+The method provided by the invention completes and supplements the blank of the original sulfur stable isotope detection technology.
Disclosure of Invention
The invention aims to solve the technical problem of providing a method for detecting stable isotopes of garlic sulfur in rachis tissue culture, which is characterized by comprising the following steps:
(1) drying, grinding and filtering a rachis tissue culture garlic bulb sample by using a screen, accurately weighing 1-10 mg of sample, filling the sample into a tin cup and wrapping the sample;
(2) detecting a sample to be detected by adopting an element analyzer-stable isotope mass spectrometer; the elemental analyzer parameters were: the oxidation tube is 1000 ℃, the column temperature box is 80 ℃, and the catalyst in the quartz tube in the oxidation furnace is 25 g of tungsten oxide and 50 g of granular pure copper;
(3) ionizing sample by high-energy ion source to generate SO+(ii) a Ion source parameters of the stable isotope mass spectrometer are as follows: the core voltage of the ion source is 5.8KV, and the pressure of carrier gas He is 15 psi;
(4) mass analyzer pair SO+AppositionSeparating the prime signal; the voltage of the first-stage quadrupole is-130V, and the voltage of the second-stage quadrupole is 158V;
(5) and analyzing the isotope value of the sample under the conditions to obtain the sulfur isotope value of the sample, repeatedly detecting for 5 times, and counting the experimental result.
The invention further discloses a method for detecting the sulfur stable isotope of the rachis tissue culture garlic, and the method is applied to the aspect of improving the accuracy of detecting the sulfur stable isotope of the rachis tissue culture garlic sample. The experimental results show that: the method has the advantages of good reproducibility, high analysis efficiency, high accuracy and the like, can meet the requirement of detecting the sulfur stable isotope of the rachis tissue culture garlic sample, the analysis accuracy SD (‰) of the sulfur stable isotope can reach 0.05 ‰, and the analysis stability SD (% o) of the sulfur stable isotope can reach 0.08 ‰, which cannot be reached by the conventional sulfur dioxide mode analysis method.
The invention mainly solves the problem of poor detection accuracy of the sulfur stable isotope of the garlic cultured by the rachis tissue, and overcomes the influence of sulfur dioxide ions with high mass-to-charge ratio on the detection of the sulfur isotope caused by the memory effect of an instrument. The method mainly considers that sulfur dioxide ions are replaced by sulfur monoxide ions with lower mass-to-charge ratio by controlling the parameters of an ion source and a mass analyzer so as to improve the accuracy and the reproducibility of detecting the sulfur isotope in the rachis tissue culture garlic sample.
Compared with the prior art, the method for detecting the stable isotope of the garlic sulfur cultured by the rachis tissue has the positive effects that:
(1) the method has the advantages of easy operation, no influence of instrument memory effect on the detection of the sulfur stable isotope ratio, high accuracy, good reproducibility and the like.
(2) Compared with the prior art, the method solves the problem that sulfur dioxide ions with high mass-to-charge ratio have influence on the detection of the sulfur isotope due to the instrument memory effect when the sulfur stable isotope in the rachis tissue culture garlic sample is detected, and improves the accuracy and the repeatability of the detection of the sulfur isotope.
Detailed Description
The invention is described below by means of specific embodiments. Unless otherwise specified, the technical means used in the present invention are well known to those skilled in the art. In addition, the embodiments should be considered illustrative, and not restrictive, of the scope of the invention, which is defined solely by the claims. It will be apparent to those skilled in the art that various changes or modifications in the components and amounts of the materials used in these embodiments can be made without departing from the spirit and scope of the invention. The raw materials and reagents used in the present invention are commercially available.
Example 1
A method for detecting sulfur stable isotope of garlic cultured by rachis tissue comprises the following steps:
a. drying, grinding and filtering a rachis tissue culture garlic bulb sample, accurately weighing 5.0mg of sample, filling the sample into a tin cup and wrapping the sample;
b. in the detection of the sulfur stable isotope (δ S34), IAEA-S-1 (silver sulfide, δ S34 ═ 0.30 ‰), IAEA-S-3 (silver sulfide, δ S34 ═ 32.3 ‰), and NBS-127 (barium sulfate, δ S34 ═ 20.3 ‰) were used as standards.
c. Detecting a sample to be detected by adopting an element analyzer-stable isotope mass spectrometer; the elemental analyzer parameters were: the oxidation tube is 1000 ℃, the column temperature box is 80 ℃, and the catalyst in the quartz tube in the oxidation furnace is 25 g of tungsten oxide and 50 g of granular pure copper.
d. Ionizing sample by high-energy ion source to generate SO+(ii) a Ion source parameters of the stable isotope mass spectrometer are as follows: the core voltage of the ion source is 5.8KV, and the pressure of the carrier gas He is 15 psi.
e. Mass analyzer pair SO+Separating isotope signals; the voltage of the first-level quadrupole is-130V, and the voltage of the second-level quadrupole is 158V.
f. And analyzing the isotope values of the standard substance and the sample under the conditions, establishing a standard curve by taking the signal value of the standard substance as a horizontal coordinate and the standard isotope value of the standard substance as a vertical coordinate, and bringing the signal value of the sample into the standard curve to obtain the sulfur isotope value of the sample. And repeating the detection for 5 times, and counting the experimental results.
g. The invention mainly uses the following instruments: nu Horizon type stable isotope mass spectrometer, EuroVector EA3000 type element analyzer.
The rachis tissue culture garlic samples were processed by the above procedure and the test was repeated 5 times, and the results are shown in table 1.
TABLE 1. results of the accuracy test of. delta. S34
Example 2
A method for detecting sulfur stable isotope of garlic cultured by rachis tissue comprises the following steps:
a. drying, grinding and filtering the rachis tissue culture garlic bulb sample by a screen, accurately weighing 1.0, 2.5, 4.0, 5.5, 7.0, 8.5 and 10.0mg of the sample respectively, filling the sample into a tin cup and wrapping the sample.
b. In the detection of the sulfur stable isotope (δ S34), IAEA-S-1 (silver sulfide, δ S34 ═ 0.30 ‰), IAEA-S-3 (silver sulfide, δ S34 ═ 32.3 ‰), and NBS-127 (barium sulfate, δ S34 ═ 20.3 ‰) were used as standards.
c. Detecting a sample to be detected by adopting an element analyzer-stable isotope mass spectrometer; the elemental analyzer parameters were: the oxidation tube is 1000 ℃, the column temperature box is 80 ℃, and the catalyst in the quartz tube in the oxidation furnace is 25 g of tungsten oxide and 50 g of granular pure copper.
d. Ionizing sample by high-energy ion source to generate SO+(ii) a Ion source parameters of the stable isotope mass spectrometer are as follows: the core voltage of the ion source is 5.8KV, and the pressure of the carrier gas He is 15 psi.
e. Mass analyzer pair SO+Separating isotope signals; the voltage of the first-level quadrupole is-130V, and the voltage of the second-level quadrupole is 158V.
f. And analyzing the isotope values of the standard substance and the sample under the conditions, establishing a standard curve by taking the signal value of the standard substance as a horizontal coordinate and the standard isotope value of the standard substance as a vertical coordinate, and bringing the signal value of the sample into the standard curve to obtain the sulfur isotope value of the sample. And repeating the detection for 5 times, and counting the experimental results.
g. The invention mainly uses the following instruments: nu Horizon type stable isotope mass spectrometer, EuroVector EA3000 type element analyzer.
The stability of the method was tested by treating the rachis tissue culture garlic samples with the above procedure and the results are shown in table 2.
TABLE 2. results of stability test of δ S34(‰)
Example 3
Comparative test
In conclusion, the method has the advantages of high precision, good repeatability, high efficiency and the like, and can improve the sulfur isotope detection capability of the garlic sample cultured by the rachis tissue.
Claims (2)
1. A method for detecting a stable isotope of garlic sulfur in tissue culture of rachis tissue culture is characterized by comprising the following steps:
(1) drying, grinding and filtering a rachis tissue culture garlic bulb sample by using a screen, accurately weighing 1-10 mg of sample, filling the sample into a tin cup and wrapping the sample;
(2) detecting a sample to be detected by adopting an element analyzer-stable isotope mass spectrometer; the elemental analyzer parameters were: the oxidation tube is 1000 ℃, the column temperature box is 80 ℃, and the catalyst in the quartz tube in the oxidation furnace is 25 g of tungsten oxide and 50 g of granular pure copper;
(3) ionizing sample by high-energy ion source to generate SO+(ii) a Ion source parameters of the stable isotope mass spectrometer are as follows: the core voltage of the ion source is 5.8KV, and the pressure of carrier gas He is 15 psi;
(4) mass analyzer pair SO+Separating isotope signals; the voltage of the first-stage quadrupole is-130V, and the voltage of the second-stage quadrupole is 158V;
(5) and (3) analyzing the sulfur isotope signals of the sample under the conditions to obtain the sulfur isotope value of the sample, repeatedly detecting for 5 times, and counting the experimental result.
2. The application of the method for detecting the stable isotope of the sulfur in the tissue culture garlic of the rachis by using the sulfur monoxide ions as claimed in claim 1 in improving the accuracy and the repeatability of the detection of the stable isotope of the sulfur in the tissue culture garlic sample of the rachis.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102010039275A1 (en) * | 2010-08-12 | 2012-02-16 | Helmholtz-Zentrum Für Umweltforschung Gmbh - Ufz | Method and apparatus for the online determination of the isotopic ratio of chlorine, bromine or sulfur in an organic sample |
| US20130110412A1 (en) * | 2011-11-02 | 2013-05-02 | Vlaamse Instelling Voor Technologisch Onderzoek (Vito) Nv | Method and device for computing molecular isotope distributions and for estimating the elemental composition of a molecule from an isotopic distribution |
| CN109406616A (en) * | 2018-09-30 | 2019-03-01 | 中国地质大学（武汉） | A method of sulfate mineral sulfur isotope is measured by standard specimen of YF-2 barite |
| KR20190048342A (en) * | 2017-10-31 | 2019-05-09 | 한국기초과학지원연구원 | Method for simultaneous analysis of stable isotope of C, N and S from agricultural products |
| CN110672709A (en) * | 2019-11-18 | 2020-01-10 | 中国水产科学研究院黄海水产研究所 | Method for analyzing particle organic carbon and nitrogen stable isotopes in seawater by EA-IRMS |
| CN111272917A (en) * | 2020-03-30 | 2020-06-12 | 中国科学院地质与地球物理研究所 | Element isotope analysis system and method for analyzing sulfur isotope in trace sulfate sample and application thereof |
-
2021
- 2021-01-18 CN CN202110060178.5A patent/CN112730576A/en not_active Withdrawn
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102010039275A1 (en) * | 2010-08-12 | 2012-02-16 | Helmholtz-Zentrum Für Umweltforschung Gmbh - Ufz | Method and apparatus for the online determination of the isotopic ratio of chlorine, bromine or sulfur in an organic sample |
| US20130110412A1 (en) * | 2011-11-02 | 2013-05-02 | Vlaamse Instelling Voor Technologisch Onderzoek (Vito) Nv | Method and device for computing molecular isotope distributions and for estimating the elemental composition of a molecule from an isotopic distribution |
| KR20190048342A (en) * | 2017-10-31 | 2019-05-09 | 한국기초과학지원연구원 | Method for simultaneous analysis of stable isotope of C, N and S from agricultural products |
| CN109406616A (en) * | 2018-09-30 | 2019-03-01 | 中国地质大学（武汉） | A method of sulfate mineral sulfur isotope is measured by standard specimen of YF-2 barite |
| CN110672709A (en) * | 2019-11-18 | 2020-01-10 | 中国水产科学研究院黄海水产研究所 | Method for analyzing particle organic carbon and nitrogen stable isotopes in seawater by EA-IRMS |
| CN111272917A (en) * | 2020-03-30 | 2020-06-12 | 中国科学院地质与地球物理研究所 | Element isotope analysis system and method for analyzing sulfur isotope in trace sulfate sample and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| KIM A. BAUBLYS: "Simultaneous determination of d33SV-CDT and d34SV-CDT using masses 48, 49 and 50 on a continuous flow isotope ratio mass spectrometer", 《RAPID COMMUN. MASS SPECTROM》 * |
| 金贵善: "硫化物中硫同位素组成的ＥＡ－ＩＲＭＳ分析方法", 《铀矿地质》 * |
| 韩娟: "样品质量对EA-IRMS法测量硫同位素组成的影响", 《铀矿地质》 * |
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Application publication date: 20210430 |