CN112730285A - High-throughput method for detecting content of ganoderma lucidum polysaccharide - Google Patents
High-throughput method for detecting content of ganoderma lucidum polysaccharide Download PDFInfo
- Publication number
- CN112730285A CN112730285A CN202011534722.7A CN202011534722A CN112730285A CN 112730285 A CN112730285 A CN 112730285A CN 202011534722 A CN202011534722 A CN 202011534722A CN 112730285 A CN112730285 A CN 112730285A
- Authority
- CN
- China
- Prior art keywords
- solution
- sulfuric acid
- ganoderma lucidum
- detecting
- phenol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a high-throughput method for detecting the content of ganoderma lucidum polysaccharide, belonging to the technical field of biological analysis. An enzyme-labeling instrument is applied to detecting the content of the ganoderma lucidum polysaccharide, and the phenol-sulfuric acid method and the anthrone-sulfuric acid method have good linear relation within the range of 10-100 mu g/mL of glucose concentration. The enzyme-labeling instrument for detecting the ganoderan has the advantages of high flux, sample saving, shortened analysis time, dynamic monitoring and the like. Provides a new method for the analysis and detection of the ganoderma lucidum polysaccharide.
Description
Technical Field
The invention discloses a high-throughput method for detecting the content of ganoderma lucidum polysaccharide. The method uses an enzyme-labeling instrument and uses a phenol-sulfuric acid method or an anthrone-sulfuric acid method to carry out high-flux detection on the ganoderma lucidum polysaccharide, and belongs to the technical field of biological analysis.
Background
Ganoderan is an important effective component in Ganoderma lucidum, is composed of three strands of monosaccharide chains, has a helical stereo configuration (tertiary structure), has a similar stereo configuration to deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), and is a macromolecular compound. Has the biological activities of reducing blood sugar, reducing blood fat, resisting thrombus, resisting oxidation, eliminating free radicals, resisting aging, resisting radiation, resisting tumor, promoting blood circulation, regulating human body immunity, etc. The ganoderma lucidum polysaccharide has special physiological activity and medical value, is safe and non-toxic, and is widely applied to the industries of medicine, food and cosmetics.
At present, the content analysis of the ganoderma lucidum polysaccharide mainly adopts a colorimetric method and a chromatography method, and reports also exist, and the relative molecular mass, chemical formula and structure of the ganoderma lucidum polysaccharide are analyzed by combining the chromatography and mass spectrometry. The above methods all have their own features and disadvantages. In particular, most researchers adopt a colorimetric method, whether phenol or anthrone needs to use a large amount of concentrated sulfuric acid as a detection solvent, a large amount of test samples need boiling water to boil the samples, preparation and test take a long time, and a large amount of acidic waste liquid is generated after the test, so that great pressure is brought to the environment or waste treatment.
The microplate reader is a device which is prepared in a biological laboratory, can generally use a 96-well plate and other samples for detection, has a temperature regulation function, and can heat the samples at constant temperature. In the teaching and scientific research experiment that adopts ordinary spectrophotometer to detect polysaccharide, we have designed new detection experiment scheme, use the ELIASA to measure ganoderan's content, can once measure a plurality of samples (by the pore plate number decision of hole quantity), can heat the sample simultaneously, avoided the potential safety hazard of heating in boiling water bath, realized ganoderan's high flux detection. And a large amount of reagents such as phenol, anthrone, sulfuric acid and the like are saved, and the generation of a large amount of acidic waste liquid is avoided.
Disclosure of Invention
The invention aims to design and provide a method for detecting the content of ganoderma lucidum polysaccharide with high flux by using a common laboratory instrument, namely an enzyme-labeling instrument through practical inspection. In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
detecting the content of the ganoderma lucidum polysaccharide by using a phenol-sulfuric acid method or an anthrone-sulfuric acid method by using an enzyme labeling instrument, wherein the method comprises the following steps:
(1) respectively mixing the glucose standard solution and the ganoderma lucidum polysaccharide sample solution with a color reagent, and placing the mixture in a 96-well plate;
(2) standing at a certain temperature for a certain time, and detecting at a fixed wavelength;
(3) drawing a glucose solution standard curve, and calculating the total sugar content in the ganoderma lucidum polysaccharide sample solution.
In the steps, more than 5 glucose standard solutions with the concentration of 10-100 mug/mL are prepared; typically the concentration of the glucose standard solution can be measured using five concentration points of 20, 40, 60, 80, and 100. mu.g/mL.
In the above method, the coloring reagent may be phenol-sulfuric acid solution or anthrone-sulfuric acid solution.
When the color reagent is phenol-sulfuric acid solution, the mixing ratio of sugar solution and 5% phenol solution is 1/1-1/2, preferably 2/3. The volume ratio of the sugar solution to the concentrated sulfuric acid is 1/4-1/5, preferably 2/9. The reaction temperature is 25 ℃, the color development time is 10-30 minutes, preferably 20 minutes, and the detection wavelength is 490 nm.
When the color reagent is anthrone-sulfuric acid solution, the volume ratio of the sugar solution to the anthrone solution is 1/2-1/4, preferably 1/3. The reaction temperature is 100 ℃, the color development time is 5-20 minutes, preferably 10 minutes, and the detection wavelength is 620 nm.
In the detection process, the highest light absorption value of each sample can be confirmed by obtaining the light absorption values at different time points by utilizing the dynamic detection function of the microplate reader. Through the dynamic investigation of the reaction of the two chromogenic reagents, parameters such as reagent mixing proportion, temperature and the like are optimized, and the stable use condition of the detection method is obtained.
The method of the invention has the following advantages: 1. on a microplate reader, the glucose standard solution within the range of 10-100 mu g/mL shows good linear relation (R) by utilizing the phenol-sulfuric acid method and the anthrone-sulfuric acid method2>0.995); 2. compared with the common spectrophotometer, the time for detection is greatly shortened, the number of samples detected in a single time is up to 96 (determined by the number of holes on a pore plate), high-flux detection is realized, and the experimental efficiency is improved; 3. the using amount of the sample and the strong acid reagent, namely concentrated sulfuric acid is reduced, the risk of experimental operation is reduced, the waste discharge is reduced, and the experimental resources are saved.
Drawings
FIG. 1 is a graph showing glucose calibration curves of phenol-sulfuric acid method and anthrone-sulfuric acid method
FIG. 2 is a graph showing the influence of different amounts of phenol solution on absorbance in the phenol-sulfuric acid method
FIG. 3 is a graph showing the effect of different concentrations of sulfuric acid on absorbance in a phenol-sulfuric acid process
FIG. 4 is a graph showing the effect of different reaction times on absorbance in the phenol-sulfuric acid method
FIG. 5 is a graph showing the effect of different anthrone reagents on absorbance in the anthrone-sulfuric acid process
FIG. 6 shows the effect of different heating times on absorbance in the anthrone-sulfuric acid process
FIG. 7 shows the stability of the phenol-sulfuric acid process and the anthrone-sulfuric acid process
Detailed Description
To further illustrate the details of the present invention, the following preferred examples are given, all using commercially available microplate reader devices, but the present invention is not limited to the specific embodiments listed below.
Example 1
Standard glucose solutions (20, 40, 60, 80, 100 μ g/mL) were pipetted separately and mixed with 5% phenol solution and concentrated sulfuric acid in 96-well plates at a volume ratio of 1: 1: and 5, oscillating the sample on an enzyme-linked immunosorbent assay (ELIASA) with the total volume of 180 mu l, wherein the reaction temperature is 25 ℃, the color development time is 30min, the absorbance is measured, the detection wavelength of the ELIASA is set at 490nm, and distilled water is used as a reagent blank. And (5) drawing a standard curve, and fitting by using a linear regression equation to obtain related parameters, wherein the standard curve is shown in the attached figure 1. The equation was then used to calculate the total sugar concentration in the polysaccharide sample solution to be 55.59 μ g/mL.
Example 2
Standard glucose solutions (20, 40, 60, 80, 100 μ g/mL) were pipetted separately and mixed with 1% anthrone concentrated sulfuric acid solution in 96-well plates at a volume ratio of 1: 3, the total volume is 180ul, the reaction temperature is 100 ℃, the color development time is 10min, the detection wavelength of an enzyme-labeling instrument is set at 620nm, and distilled water is used as a reagent blank. And (5) drawing a standard curve, and fitting by using a linear regression equation to obtain related parameters, wherein the standard curve is shown in the attached figure 1. Then, the total sugar concentration in the polysaccharide sample solution was calculated to be 55.27. mu.g/mL using this equation.
Example 3
Taking a glucose standard solution as a research object, taking absorbance as a response value, and mixing the glucose standard solution, a 5% phenol solution and concentrated sulfuric acid in a 96-well plate under the condition of 490nm wavelength, wherein the volume ratio is 1: 1.25: and 5, oscillating the mixture on a microplate reader with the total volume of 180 mu l, reacting at the temperature of 25 ℃, developing for 30min, and measuring the absorbance, wherein the result is shown in the attached figure 2.
Example 4
Taking a glucose standard solution as a research object, taking absorbance as a response value, and mixing the glucose standard solution, a 5% phenol solution and concentrated sulfuric acid in a 96-well plate under the condition of 490nm wavelength, wherein the volume ratio is 1: 1.5: and 5, oscillating the mixture on a microplate reader with the total volume of 180 mu l, reacting at the temperature of 25 ℃, developing for 30min, and determining the absorbance. As shown in the attached figure 2, the volume ratio of the glucose standard solution to the 5% phenol solution is 1: the best is 1.5.
Example 5
Taking a glucose standard solution as a research object, taking absorbance as a response value, and mixing the glucose standard solution, a 5% phenol solution and concentrated sulfuric acid in a 96-well plate under the condition of 490nm wavelength, wherein the volume ratio is 1: 1.75: and 5, oscillating the mixture on a microplate reader with the total volume of 180 mu l, reacting at the temperature of 25 ℃, developing for 30min, and measuring the absorbance, wherein the result is shown in the attached figure 2.
Example 6
Taking a glucose standard solution as a research object, taking absorbance as a response value, and mixing the glucose standard solution, a 5% phenol solution and concentrated sulfuric acid in a 96-well plate under the condition of 490nm wavelength, wherein the volume ratio is 1: 2: and 5, oscillating the mixture on a microplate reader with the total volume of 180 mu l, reacting at the temperature of 25 ℃, developing for 30min, and measuring the absorbance, wherein the result is shown in the attached figure 2.
Example 7
Taking a glucose standard solution as a research object, taking absorbance as a response value, and mixing the glucose standard solution, a 5% phenol solution and concentrated sulfuric acid in a 96-well plate under the condition of 490nm wavelength, wherein the volume ratio is 1: 1: 4, the total volume is 180 mul, the shaking is carried out on a microplate reader, the reaction temperature is 25 ℃, the color development time is 30min, the absorbance is measured, and the result is shown in figure 3.
Example 8
Taking a glucose standard solution as a research object, taking absorbance as a response value, and mixing the glucose standard solution, a 5% phenol solution and concentrated sulfuric acid in a 96-well plate under the condition of 490nm wavelength, wherein the volume ratio is 1: 1: 4.25, the total volume is 180 mul, the shaking is carried out on a microplate reader, the reaction temperature is 25 ℃, the color development time is 30min, the absorbance is measured, and the result is shown in figure 3.
Example 9
Taking a glucose standard solution as a research object, taking absorbance as a response value, and mixing the glucose standard solution, a 5% phenol solution and concentrated sulfuric acid in a 96-well plate under the condition of 490nm wavelength, wherein the volume ratio is 1: 1: 4.5, the total volume is 180 mul, the shaking is carried out on a microplate reader, the reaction temperature is 25 ℃, the color development time is 30min, and the absorbance is measured. As shown in the attached figure 3, the volume ratio of the glucose standard solution to the concentrated sulfuric acid is 1: the best is 4.5.
Example 10
Taking a glucose standard solution as a research object, taking absorbance as a response value, and mixing the glucose standard solution, a 5% phenol solution and concentrated sulfuric acid in a 96-well plate under the condition of 490nm wavelength, wherein the volume ratio is 1: 1: 4.75, the total volume is 180 mul, the shaking is carried out on a microplate reader, the reaction temperature is 25 ℃, the color development time is 30min, the absorbance is measured, and the result is shown in figure 3.
Example 11
Taking a glucose standard solution as a research object, taking absorbance as a response value, and under the condition of 490nm wavelength, mixing the glucose standard solution with a 5% phenol solution and a sulfuric acid solution according to the ratio of 1: 1: mixing in 96-well plate at volume ratio of 5, total volume of 180 μ l, reaction temperature of 25 deg.C, color development time of 20min, and measuring absorbance, the result is shown in figure 4.
Example 12
Taking a glucose standard solution as a research object, taking absorbance as a response value, and under the condition of 490nm wavelength, mixing the glucose standard solution with a 5% phenol solution and a sulfuric acid solution according to the ratio of 1: 1: mixing in 96-well plate at volume ratio of 5, total volume of 180 μ l, reaction temperature of 25 deg.C, color development time of 25min, and measuring absorbance, the result is shown in figure 4.
Example 13
Taking a glucose standard solution as a research object, taking absorbance as a response value, and mixing the glucose standard solution, a 5% phenol solution and concentrated sulfuric acid in a 96-well plate under the condition of 490nm wavelength, wherein the volume ratio is 1: 1: and 5, oscillating the mixture on a microplate reader with the total volume of 180 mu l, reacting at the temperature of 25 ℃, developing for 30min, and determining the absorbance. As shown in FIG. 4, the development time is preferably 30 min.
Example 14
Taking a glucose standard solution as a research object, taking absorbance as a response value, and under the condition of 490nm wavelength, mixing the glucose standard solution with a 5% phenol solution and a sulfuric acid solution according to the ratio of 1: 1: mixing in 96-well plate at volume ratio of 5, total volume of 180 μ l, reaction temperature of 25 deg.C, color development time of 35min, and measuring absorbance, the result is shown in figure 4.
Example 15
Taking a glucose standard solution as a research object, taking absorbance as a response value, and under the condition of 490nm wavelength, mixing the glucose standard solution with a 5% phenol solution and a sulfuric acid solution according to the ratio of 1: 1: mixing in 96-well plate at volume ratio of 5, total volume of 180 μ l, reaction temperature of 25 deg.C, color development time of 40min, and measuring absorbance, the result is shown in figure 4.
Example 16
Taking a glucose standard solution as a research object, taking absorbance as a response value, and under the condition of a wavelength of 620nm, the volume ratio of the glucose standard solution to the anthrone solution is 1: 2, the total volume is 180ul, the reaction temperature is 100 ℃, the color development time is 10min, and the absorbance is measured, and the result is shown in figure 5.
Example 17
Taking a glucose standard solution as a research object, taking absorbance as a response value, and under the condition of a wavelength of 620nm, the volume ratio of the glucose standard solution to the anthrone solution is 1: 2.5, the total volume is 180ul, the reaction temperature is 100 ℃, the color development time is 10min, and the absorbance is measured, and the result is shown in figure 5.
Example 18
Taking a glucose standard solution as a research object, taking absorbance as a response value, and under the condition of a wavelength of 620nm, the volume ratio of the glucose standard solution to the anthrone solution is 1: 3, the total volume is 180ul, the reaction temperature is 100 ℃, the color development time is 10min, and the absorbance is measured. As shown in the attached figure 5, the volume ratio of the glucose standard solution to the anthrone solution is 1: the best is 3 times.
Example 19
Taking a glucose standard solution as a research object, taking absorbance as a response value, and under the condition of a wavelength of 620nm, the volume ratio of the glucose standard solution to the anthrone solution is 1: 3.5, the total volume is 180ul, the reaction temperature is 100 ℃, the color development time is 10min, the absorbance is measured, and the result is shown in figure 5.
Example 20
Taking a glucose standard solution as a research object, taking absorbance as a response value, and under the condition of a wavelength of 620nm, the volume ratio of the glucose standard solution to the anthrone solution is 1: 4, the total volume is 180ul, the reaction temperature is 100 ℃, the color development time is 10min, the absorbance is measured, and the result is shown in figure 5.
Example 21
Taking a glucose standard solution as a research object, taking absorbance as a response value, and setting the volume ratio of the glucose standard solution to the anthrone reagent to be 1: 3, mixing in 96-well plate, reacting at 100 deg.C for 1min in total volume of 180 μ l, and measuring absorbance, the result is shown in figure 6.
Example 22
Taking a glucose standard solution as a research object, taking absorbance as a response value, and setting the volume ratio of the glucose standard solution to the anthrone reagent to be 1: 3, mixing in a 96-well plate, reacting at 100 deg.C for 3min in a total volume of 180 μ l, and measuring absorbance, the result is shown in figure 6.
Example 23
Taking a glucose standard solution as a research object, taking absorbance as a response value, and setting the volume ratio of the glucose standard solution to the anthrone reagent to be 1: 3, mixing in a 96-well plate, reacting at 100 deg.C for 5min in total volume of 180 μ l, and measuring absorbance, with the result shown in figure 6.
Example 24
Taking a glucose standard solution as a research object, taking absorbance as a response value, and setting the volume ratio of the glucose standard solution to the anthrone reagent to be 1: 3, mixing in a 96-well plate, reacting at 100 deg.C for 15min in total volume of 180 μ l, and measuring absorbance, with the result shown in figure 6.
Example 25
Placing ganoderan solution in 96-well plate, performing color reaction by phenol-sulfuric acid method and anthrone-sulfuric acid method, monitoring kinetics with enzyme labeling instrument, detecting every 5min, and determining stability of the two methods. The results are shown in figure 7, which shows that the stability of the phenol-sulfuric acid method is superior to that of the anthrone-sulfuric acid method.
Claims (10)
1. A high-throughput method for detecting the content of ganoderma lucidum polysaccharide is characterized in that: the method comprises the following steps:
(1) respectively mixing the glucose standard solution and the ganoderma lucidum polysaccharide sample solution with a color reagent, and placing the mixture in a 96-well plate;
(2) standing at a certain temperature for a certain time, and detecting at a fixed wavelength;
(3) and drawing a glucose solution standard curve, and calculating the total sugar content in the ganoderma lucidum polysaccharide sample.
2. The high-throughput method for detecting the content of ganoderma lucidum polysaccharide according to claim 1, wherein the method comprises the following steps: the color reagent can be phenol-sulfuric acid solution or anthrone-sulfuric acid solution.
3. The high-throughput method for detecting the content of ganoderma lucidum polysaccharide according to claim 2, wherein the method comprises the following steps: when the color reagent is phenol-sulfuric acid solution, the mixing ratio of sugar solution and 5% phenol solution is 1/1-1/2.
4. The high-throughput method for detecting the content of ganoderma lucidum polysaccharide according to claim 2, wherein the method comprises the following steps: when the color reagent is phenol-sulfuric acid solution, the mixing ratio of the sugar solution and 5% phenol solution is 2/3.
5. The high-throughput method for detecting the content of ganoderma lucidum polysaccharide according to claim 2, wherein the method comprises the following steps: when the color reagent is phenol-sulfuric acid solution, the volume ratio of the sugar solution to the concentrated sulfuric acid is 1/4-1/5.
6. The high-throughput method for detecting the content of ganoderma lucidum polysaccharide according to claim 2, wherein the method comprises the following steps: when the color reagent is phenol-sulfuric acid solution, the mixing ratio of sugar solution and concentrated sulfuric acid is 2/9.
7. The high-throughput method for detecting the content of ganoderma lucidum polysaccharides according to claim 2, wherein when the chromogenic reagent is an anthrone-sulfuric acid solution, the volume ratio of the sugar solution to the anthrone solution is 1/2-1/4.
8. The high-throughput method for detecting the content of ganoderma lucidum polysaccharide according to claim 2, wherein when the reagent for color development is anthrone-sulfuric acid solution, the volume ratio of the sugar solution to the anthrone solution is 1/3.
9. The high-throughput method for detecting the content of ganoderma lucidum polysaccharide according to claim 1, wherein the method comprises the following steps: the temperature of the color reagent is 25 ℃ when the phenol-sulfuric acid solution is used, the color development time is 30 minutes, and the wavelength is 490 nm.
10. The high-throughput method for detecting the content of ganoderma lucidum polysaccharide according to claim 1, wherein the method comprises the following steps: the color reagent is prepared from anthrone-sulfuric acid solution at 100 deg.C for 10min, and has wavelength of 620 nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011534722.7A CN112730285A (en) | 2020-12-22 | 2020-12-22 | High-throughput method for detecting content of ganoderma lucidum polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011534722.7A CN112730285A (en) | 2020-12-22 | 2020-12-22 | High-throughput method for detecting content of ganoderma lucidum polysaccharide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112730285A true CN112730285A (en) | 2021-04-30 |
Family
ID=75604300
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011534722.7A Pending CN112730285A (en) | 2020-12-22 | 2020-12-22 | High-throughput method for detecting content of ganoderma lucidum polysaccharide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112730285A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4769363A (en) * | 1984-03-08 | 1988-09-06 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Beta-glucan |
| CN101477038A (en) * | 2009-01-15 | 2009-07-08 | 宋秋兰 | Method for measuring ganoderma polyoses content in ganoderma products by sulfuric acid-phynol method |
| CN103616339A (en) * | 2013-11-07 | 2014-03-05 | 培力(南宁)药业有限公司 | Detection method for preparation containing ganoderan |
| CN103901031A (en) * | 2014-04-14 | 2014-07-02 | 中国农业科学院兰州畜牧与兽药研究所 | Method for rapid and high-flux determination of polysaccharide content based on sulfuric acid and phenol |
| US20160317594A1 (en) * | 2015-04-30 | 2016-11-03 | Yuming Huang | Method for breaking sporoderm of ganoderma spore powder and products obtained by the same |
| CN107158739A (en) * | 2017-05-05 | 2017-09-15 | 独山县军鹏农产品有限责任公司 | A kind of extracting method of the high ganoderma lucidum of polyoses content |
| CN111257254A (en) * | 2020-03-19 | 2020-06-09 | 中国水产科学研究院黄海水产研究所 | Method for measuring glycogen content in oyster tissue |
-
2020
- 2020-12-22 CN CN202011534722.7A patent/CN112730285A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4769363A (en) * | 1984-03-08 | 1988-09-06 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Beta-glucan |
| CN101477038A (en) * | 2009-01-15 | 2009-07-08 | 宋秋兰 | Method for measuring ganoderma polyoses content in ganoderma products by sulfuric acid-phynol method |
| CN103616339A (en) * | 2013-11-07 | 2014-03-05 | 培力(南宁)药业有限公司 | Detection method for preparation containing ganoderan |
| CN103901031A (en) * | 2014-04-14 | 2014-07-02 | 中国农业科学院兰州畜牧与兽药研究所 | Method for rapid and high-flux determination of polysaccharide content based on sulfuric acid and phenol |
| US20160317594A1 (en) * | 2015-04-30 | 2016-11-03 | Yuming Huang | Method for breaking sporoderm of ganoderma spore powder and products obtained by the same |
| CN107158739A (en) * | 2017-05-05 | 2017-09-15 | 独山县军鹏农产品有限责任公司 | A kind of extracting method of the high ganoderma lucidum of polyoses content |
| CN111257254A (en) * | 2020-03-19 | 2020-06-09 | 中国水产科学研究院黄海水产研究所 | Method for measuring glycogen content in oyster tissue |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| HuLTMAN | Rapid specific method for determination of aldosaccharides in body fluids | |
| CN104964939A (en) | Method for detecting content of sodium and potassium ions in Danhong injection | |
| Yuen et al. | Comparison of the glucose oxidase method for glucose determination by manual assay and automated analyzer | |
| CN111239125A (en) | Glucose colorimetric detection method by using platinum disulfide | |
| CN112986231A (en) | High-throughput method for measuring content of tremella polysaccharide | |
| CN112730285A (en) | High-throughput method for detecting content of ganoderma lucidum polysaccharide | |
| CN1912626A (en) | Preparation of disposable carbon-base glucose chip and electrochemical detection method | |
| CN107561070B (en) | A kind of glucose quick visualization detection method and its preparation and application | |
| CN103115882A (en) | Method for detecting H2O2 | |
| CN115287332A (en) | Poly (A) polymerase activity determination method | |
| CN115436305A (en) | Novel method for detecting thiocyanate in vegetables | |
| CN107957403B (en) | Method for measuring chitosan content by using carmine as probe through ultraviolet spectrophotometry | |
| CN112608744A (en) | Green fluorescent carbon dot, preparation method thereof and application thereof in rutin detection | |
| CN103149190B (en) | Toilet intelligent health care detection system | |
| CN101451958B (en) | Detecting method for Xylanase efficiency fordecomposing wheat | |
| Sulistiyowati et al. | The Comparison of Blood Glucose Check Results Using Photometer and Glucometer | |
| CN108195808B (en) | Method for detecting oversulfated chondroitin sulfate impurities in heparin sodium | |
| CN112630440B (en) | Reusable glucose detection box and detection method thereof | |
| Ficorella et al. | A multi-frequency system for glucose detection with optical sensors | |
| CN114002172B (en) | Method for visually evaluating quality of royal jelly | |
| A’in et al. | Uric acid analysis using AgNO3 with UV-Vis spectrophotometry method | |
| Al-Shukry et al. | Simultaneous Determination of Iron and Copper in Aqueous Solution Using Spectrophotometry | |
| Oteiza et al. | Reaction-rate method for the determination of hydrocortisone | |
| Hussain et al. | A novel Spectrophotometric method for the trace analysis of glucose | |
| Planková et al. | Determination of selenium in clinical plasma samples related to atopic dermatitis study by chronopotentiometric stripping method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20210430 |
|
| WD01 | Invention patent application deemed withdrawn after publication |