Culture apparatus for photosynthetic microorganisms
Technical Field
The invention relates to the technical field of photosynthetic microorganism culture, in particular to a culture device for photosynthetic microorganisms.
Background
Photosynthetic microorganisms are microorganisms that live with light as the sole or primary energy source, are widely distributed in nature, and are CO fixed annually by photosynthesis2About 40% of the global net photosynthetic yield, plays an important role in energy conversion and carbon element cycle, and moreover, photosynthetic microorganisms can form various forms of biological energy through biotransformation or post-processing, thus having great economic and research values.
When a biological culture study of photosynthetic microorganisms is performed, the microorganisms are usually cultured in a culture medium in a culture dish, and the photosynthetic microorganisms need to be turned off in the growth process, but the normal growth of the photosynthetic microorganisms is affected by over-strong or over-weak illumination, so that the intensity of illumination light needs to be correspondingly changed when the intensity of external light is changed, so as to ensure that the photosynthetic microorganisms grow under the condition of proper light intensity.
Disclosure of Invention
The invention aims to solve the defects in the prior art, such as: when a biological culture study of photosynthetic microorganisms is performed, the microorganisms are usually cultured in a culture medium in a culture dish, and the photosynthetic microorganisms need to be turned off in the growth process, but the normal growth of the photosynthetic microorganisms is affected by over-strong or over-weak illumination, so that the intensity of illumination light needs to be correspondingly changed when the intensity of external light is changed, so as to ensure that the photosynthetic microorganisms grow under the condition of proper light intensity.
In order to achieve the purpose, the invention adopts the following technical scheme:
the utility model provides a photosynthetic microorganism culture apparatus, includes the culture dish, the culture dish comprises shell and inner shell two parts, be equipped with the culture chamber in the inner shell, be equipped with first cavity between shell and the inner shell, the inner shell is close to the first transparent elastic diaphragm of fixedly connected with on the lateral wall of shell, be equipped with the ware lid on the culture dish, be equipped with the second cavity in the ware lid, the transparent elastic diaphragm of fixedly connected with second on the inside wall of second cavity, all be equipped with the pure water in first cavity and the second cavity, the transparent elastic diaphragm of second is close to the culture dish setting, be equipped with portable fixing device between culture dish and the ware lid, one side of culture dish and ware lid is equipped with light intensity adjusting device.
Preferably, a groove is formed in one side wall, close to the dish cover, of the shell, a rubber pad is arranged in the groove, and a sealing ring is fixedly connected to one side wall, close to the shell, of the dish cover and is inserted into the groove.
Preferably, portable fixing device includes a plurality of iron poles that are the symmetry and set up, and is a plurality of the equal fixed connection of iron pole is on the lateral wall of shell, fixedly connected with collar on the lateral wall of ware lid, be equipped with the spout on the collar, the spout internal rotation is connected with the change, the change is the alternating expression concatenation by two sections connecting rods and two sections bar magnet and forms.
Preferably, a reflector is fixedly connected to one side wall of the outer shell close to the inner shell, and the reflector is arranged in a curved surface.
Preferably, light intensity adjusting device includes the liquid reserve tank, two dead levers of symmetry fixedly connected with on the inside wall of liquid reserve tank, two fixedly connected with screw ring between the dead lever, screw ring internal thread is connected with the threaded rod, one of threaded rod serves fixedly connected with knob, the threaded rod is kept away from one of knob and is served and rotate and be connected with the spacing ring, fixedly connected with slip piston on the lateral wall that the knob was kept away from to the spacing ring, slip piston sealing sliding connection is in the liquid reserve tank, all be equipped with the shunt tubes on slip piston and the liquid reserve tank, all be equipped with the connecting pipe on dish lid and the shell, be connected with the pipe between shunt tubes and the connecting pipe, slip piston is kept away from and is equipped with geometric proportion diverging device on a lateral wall of spacing.
Preferably, geometric proportion diverging device includes first spring, reposition of redundant personnel piston and second spring, first spring fixed connection is on the lateral wall that the spacing ring was kept away from to the slip piston, reposition of redundant personnel piston sealing sliding connection is in the liquid reserve tank, the one end fixed connection that the slip piston was kept away from to reposition of redundant personnel piston and first spring, second spring fixed connection is on the bottom side inner wall of liquid reserve tank, the one end and the reposition of redundant personnel piston fixed connection of second spring.
Compared with the prior art, the invention has the beneficial effects that:
1. one side of first transparent elastic film and the transparent elastic film of second is the pure water, the opposite side is the air, under the dual function of water pressure and atmospheric pressure, first transparent elastic film and the transparent elastic film of second all are the curved surface setting, like this, the pure water in first cavity and the second cavity, just separated into the appearance of plano-concave lens, consequently when having light to see through culture dish and dish lid shine into the culture intracavity, these "pure water plano-concave lens" just can disperse incident light, reduce light intensity, prevent to restrain photosynthetic microorganism's photosynthesis and normal growth on the contrary because of light is too strong, and the pure water still has good heat preservation effect, can be fine maintain the interior temperature of culture dish.
2. First spring, reposition of redundant personnel piston and second spring's setting, when guaranteeing that the sliding piston moves, can be with the help of elastic force, draw the pure water or inject the pure water into first cavity and second cavity from first cavity and second cavity simultaneously, so that adjust the degree of curvature of first transparent elastic film and second transparent elastic film simultaneously, the ratio of the coefficient of stiffness between second spring and the first spring equals the ratio of surface area between first transparent elastic film and the second transparent elastic film, can guarantee like this to adjust the degree of curvature of first transparent elastic film and second transparent elastic film the same, so that shine into the light intensity in the culture chamber evenly.
3. The light which originally should shine on the bottom side wall of the first cavity in the incident light on the side edge of the culture dish is reflected to the bottom of the culture cavity by the reflector, so that the situation that the photosynthetic microorganisms positioned at the bottom cannot receive sufficient light due to the layering phenomenon of the photosynthetic microorganisms is prevented.
4. When the intensity of external incident light changes, can twist the knob, drive the slip piston and reciprocate, take out or inwards pour into the pure water through shunt tubes, pipe and connecting pipe in with first cavity and the second cavity pure water into to change the camber of first transparent film and second transparent film, change the concavity of plano-concave lens concave surface, so that the dispersion effect to light of corresponding change under the external light intensity change condition, guarantee photosynthetic microorganism grows under the light intensity condition that suits.
Drawings
FIG. 1 is a sectional view showing the internal structure of a culture apparatus for photosynthetic microorganisms according to the present invention;
FIG. 2 is a schematic front view of a culture apparatus for photosynthetic microorganisms according to the present invention;
FIG. 3 is a schematic structural view of a mounting ring and a swivel of a culture apparatus for photosynthetic microorganisms according to the present invention.
In the figure: the culture dish comprises a culture dish 1, an outer shell 2, an inner shell 3, a culture cavity 4, a first cavity 5, a first transparent elastic diaphragm 6, a dish cover 7, a second cavity 8, a transparent elastic diaphragm 9, an iron rod 10, a mounting ring 11, a sliding chute 12, a rotating ring 13, a connecting rod 14, a bar magnet 15, a reflector 16, a liquid storage tank 17, a fixing rod 18, a threaded ring 19, a threaded rod 20, a knob 21, a limiting ring 22, a sliding piston 23, a shunt tube 24, a connecting tube 25, a first spring 26, a shunt piston 27 and a second spring 28.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-3, a culture apparatus for photosynthetic microorganisms, including culture dish 1, culture dish 1 comprises shell 2 and inner shell 3 two parts, be equipped with culture chamber 4 in the inner shell 3, be equipped with first cavity 5 between shell 2 and the inner shell 3, first transparent elastic diaphragm 6 of fixedly connected with on the inner shell 3 is close to a lateral wall of shell 2, first transparent elastic diaphragm 6 is that the annular sets up, be equipped with dish lid 7 on the culture dish 1, be equipped with the recess on a lateral wall of shell 2 near dish lid 7, be equipped with the rubber pad in the recess, fixedly connected with sealing ring on the lateral wall of dish lid 7 near shell 2, the sealing ring is pegged graft in the recess.
With 7 backs of lids on culture dish 1 of ware lid, can be with the help of the sealing ring, the setting of recess and rubber pad, come to seal the connection between the two, be equipped with second cavity 8 in the ware lid 7, the transparent elastic diaphragm of fixedly connected with second 9 on the inside wall of second cavity 8, all be equipped with the pure water in first cavity 5 and the second cavity 8, the transparent elastic diaphragm of second 9 is close to the setting of culture dish 1, one side of first transparent elastic film 6 and the transparent elastic film of second 9 is the pure water, the opposite side is the air, under the dual function of water pressure and atmospheric pressure, first transparent elastic film 6 and the transparent elastic film of second 9 all are the curved surface setting.
Like this, the pure water in first cavity 5 and the second cavity 8, just separated into the appearance of plano-concave lens, consequently when having light transmission culture dish 1 and dish lid 7 to shine into culture chamber 4, these "pure water plano-concave lens" just can diverge incident light, reduce light intensity, prevent to restrain photosynthetic microorganism's photosynthesis and normal growth on the contrary because of light is too strong, and the pure water still has good heat preservation effect, can be fine maintain 1 interior temperature of culture dish, be equipped with portable fixing device between culture dish 1 and the dish lid 7, portable fixing device includes a plurality of iron poles 10 that are the symmetry and set up, the equal fixed connection of a plurality of iron poles 10 is on the lateral wall of shell 2, fixedly connected with collar 11 on the lateral wall of lid 7.
The mounting ring 11 is provided with a sliding groove 12, the sliding groove 12 is rotationally connected with a rotating ring 13, the rotating ring 13 is formed by splicing two sections of connecting rods 14 and two sections of bar magnets 15 in a staggered manner, the dish cover 7 is tightly pressed on the culture dish 1 under the action of magnetic force between the bar magnets 15 and the iron rod 10, the dish cover 7 is fixed, when the dish cover 7 needs to be opened, the dish cover 7 can be conveniently taken down only by screwing the rotating ring 13 to ensure that the iron rod 10 and the bar-shaped magnet 15 are staggered, therefore use very convenient, fixedly connected with reflector 16 on the shell 2 is close to the lateral wall of inner shell 3, and reflector 16 is the curved surface setting, utilizes reflector 16 to reflect the bottom of culture chamber 4 with the light that should shine on the lateral wall at the bottom of first cavity 5 in the incident light of culture dish 1 side, prevents to appear the condition that the photosynthetic microorganism that is located the bottom can't receive sufficient light because of photosynthetic microorganism's stratification phenomenon.
One side of culture dish 1 and dish lid 7 is equipped with light intensity adjusting device, light intensity adjusting device includes liquid reserve tank 17, two dead levers 18 of symmetry fixedly connected with on the inside wall of liquid reserve tank 17, fixedly connected with screw ring 19 between two dead levers 18, 19 threaded ring female connection has threaded rod 20, threaded rod 20 serves one and serves fixedly connected with knob 21, threaded rod 20 is kept away from and is rotated in the one end of knob 21 and is connected with spacing ring 22, fixedly connected with sliding piston 23 on the lateral wall that knob 21 was kept away from to spacing ring 22, sliding piston 23 seals sliding connection in liquid reserve tank 17, all be equipped with shunt tubes 24 on sliding piston 23 and the liquid reserve tank 17, all be equipped with connecting pipe 25 on dish lid 7 and the shell 2, be connected with the pipe between shunt tubes 24 and the connecting pipe 25.
An equal ratio shunt device is arranged on one side wall of the sliding piston 23 far away from the limit ring 22 and comprises a first spring 26, a shunt piston 27 and a second spring 28, the first spring 26 is fixedly connected to one side wall of the sliding piston 23 far away from the limit ring 22, the shunt piston 27 is connected in the liquid storage box 17 in a sealing and sliding manner, the shunt piston 27 is fixedly connected with one end of the first spring 26 far away from the sliding piston 23, the second spring 28 is fixedly connected to the inner wall of the bottom side of the liquid storage box 17, the shunt piston 27 of the second spring 28 is fixedly connected, the first spring 26, the shunt piston 27 and the second spring 28 are arranged, so as to ensure that the sliding piston 23 can move under the action of elastic force, pure water is simultaneously sucked from the first and second cavities 5 and 8 or injected into the first and second cavities 5 and 8, so as to adjust the degree of bending of the first transparent elastic film 6 and the second transparent elastic film 9 at the same time.
The ratio of the stiffness coefficient between the second spring 28 and the first spring 26 is equal to the ratio of the surface area between the first transparent elastic film 6 and the second transparent elastic film 9, so that it can be ensured that the bending degrees of the first transparent elastic film 6 and the second transparent elastic film 9 are adjusted to be the same, so that the intensity of light irradiated into the culture chamber 4 is uniform, when the intensity of external incident light changes, in order to ensure that photosynthetic microorganisms grow under the appropriate light intensity condition, the intensity of light irradiated into the culture chamber needs to be correspondingly adjusted, at this time, the knob 21 can be screwed, the threaded rod 20 is driven to rotate by the knob 21, and the sliding piston 23 is driven to move upwards or downwards by virtue of the threaded connection between the threaded rod 20 and the threaded ring 19.
When corotation knob 21 drives sliding piston 23 to move up, the purified water in first cavity 5 and second cavity 8 can be pumped out through shunt tubes 24, pipe and connecting pipe 25, under the effect of air pressure, the camber of first transparent film 6 and second transparent film 9 increases, thereby make the concavity of plano-concave lens concave surface increase, so that the dispersion effect to light is strengthened under the circumstances that external light intensity strengthens, when reversal knob 21 drives sliding piston 23 to move down, purified water can be injected into first cavity 5 and second cavity 8 through shunt tubes 24, pipe and connecting pipe 25, under the effect of water pressure, the air is further compressed, the camber of first transparent film 6 and second transparent film 9 reduces, thereby make the concavity of plano-concave lens concave surface reduce, so that the dispersion effect to light is weakened under the circumstances that external light intensity weakens.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.