CN112717513A - Centrifuging tube suction filter device - Google Patents
Centrifuging tube suction filter device Download PDFInfo
- Publication number
- CN112717513A CN112717513A CN202011537454.4A CN202011537454A CN112717513A CN 112717513 A CN112717513 A CN 112717513A CN 202011537454 A CN202011537454 A CN 202011537454A CN 112717513 A CN112717513 A CN 112717513A
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- CN
- China
- Prior art keywords
- centrifuge tube
- suction filtration
- connecting seat
- tube
- connecting groove
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000967 suction filtration Methods 0.000 claims abstract description 30
- 239000012528 membrane Substances 0.000 claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 230000002093 peripheral effect Effects 0.000 claims description 2
- 238000001914 filtration Methods 0.000 abstract description 8
- 238000000034 method Methods 0.000 description 6
- 238000012864 cross contamination Methods 0.000 description 4
- 230000007547 defect Effects 0.000 description 4
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 238000003828 vacuum filtration Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/01—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements
- B01D29/05—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements supported
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Centrifugal Separators (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention provides a centrifugal tube suction filtration device which comprises a first centrifugal tube, a second centrifugal tube, a connecting assembly and a filtering assembly, wherein the first centrifugal tube is connected with the second centrifugal tube through the connecting assembly; the connecting assembly comprises a first connecting seat and a second connecting seat; the filtering component comprises a filter membrane and a supporting plate, and the filter membrane is arranged on the supporting plate; the first connecting seat is provided with a first connecting groove and a second connecting groove, the second connecting seat is provided with a third connecting groove and a connecting boss, the first centrifugal tube is connected in the first connecting groove, the connecting boss can extend into the second connecting groove and tightly press the fixed supporting plate, and the second centrifugal tube is connected in the third connecting groove; one side of the first connecting seat is provided with a vent hole communicated with the inside of the first centrifugal pipe, one side of the second connecting seat is provided with an air suction hole communicated with the inside of the second centrifugal pipe, and the connecting boss is provided with a through liquid guide pipe; the invention has the advantages of simple structure, convenient operation and replaceable filter membrane, and is particularly suitable for the suction filtration of a small volume of solution.
Description
Technical Field
The invention belongs to the technical field of suction filtration, and particularly relates to a centrifugal tube suction filtration device.
Background
The suction filtration device among the prior art mainly includes: the filter cup, the funnel, the filter membrane, the glass cover and the filter flask are improved or combined aiming at the local part of the vacuum filtration device, so that the use convenience is improved to a certain extent, and some defects of the vacuum filtration device are overcome; however, the defects that the suction filtration device is too large, the suction filtration of a small volume of solution cannot be carried out, the operation is complicated, the steps are multiple, the loss in the solution transfer process is large and the like still exist.
The sample filtering steps of the conventional suction filtration device at present are generally as follows: the sample is poured into the filter cup, enters the filter flask through the filter membrane and is transferred to the sample flask from the filter flask, the process at least needs two times of sample transfer, the loss and possible cross contamination of the sample filtration process are increased, and the sample loss and the cross contamination are easily caused by the suction filtration of a small volume of solution.
Based on this, need design a suction filtration device that directly realizes the intercommunication of two intraductal samples of centrifugation, when sparingly empting liquid and shift liquid, solve the problem that probably has cross contamination.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a centrifugal tube suction filtration device which adopts a straight-through structure to carry out suction filtration while transferring a sample in two centrifugal tubes, has the advantages of replaceable filter membrane, simple structure and convenient operation, and is particularly suitable for suction filtration of a small volume of solution.
In order to achieve the above object, the present invention provides a centrifugal tube suction filtration apparatus, comprising:
a first centrifuge tube;
a second centrifuge tube;
the first centrifugal tube and the second centrifugal tube are connected through the connecting assembly;
the connecting assembly comprises a first connecting seat and a second connecting seat which are connected with each other, the first connecting seat and the first centrifugal tube are detachably connected into a whole, and the second connecting seat and the second centrifugal tube are detachably connected together;
and
a filter assembly;
the filtering component comprises a filter membrane and a supporting plate, and the filter membrane is arranged on the supporting plate;
the first connecting seat is provided with a first connecting groove and a second connecting groove, the second connecting seat is provided with a third connecting groove and a connecting boss, the first centrifugal tube is connected in the first connecting groove, the connecting boss can extend into the second connecting groove and compress the fixed supporting plate, and the second centrifugal tube is connected in the third connecting groove;
wherein, one side of the first connecting seat is provided with a vent hole communicated with the inside of the first centrifugal tube, one side of the second connecting seat is provided with an air suction hole communicated with the inside of the second centrifugal tube, and the connecting boss is provided with a through liquid guide tube;
the vacuum pump connected with the air suction hole keeps the negative pressure state in the second centrifugal tube, and the sample in the first centrifugal tube passes through the filter membrane and the liquid guide tube and enters the second centrifugal tube.
In the technical scheme of the invention, the sample in the first centrifugal tube can pass through the filter membrane from the first connecting groove, then pass through the catheter and enter the second centrifugal tube from the third connecting groove, and the suction filtration is realized while the sample is transferred.
Furthermore, the filter cup in the traditional filter device is omitted, the filter cup is simplified into the first centrifuge tube and the connecting assembly which can be assembled into a whole, the traditional liquid adding mode is further replaced, the filter flask in the traditional filter device is omitted, the filter flask and the second centrifuge tube are integrated, the defect that a sample needs to be transferred is overcome, the device is simplified, the operation is more convenient, and the suction filtration process can be completed only through single sample transfer.
Specifically, the invention adopts a modularized assembly structure, the filter membrane can be replaced according to different samples, and the invention has good adaptability and strong practicability.
According to another embodiment of the invention, the first connecting groove and the second connecting groove are shrunk to form a snap ring-shaped limiting structure, and the assembled connecting boss presses the support plate on the snap ring-shaped limiting structure.
According to another embodiment of the invention, the connecting boss is recessed downwards to form a diversion trench, and the diversion trench is beneficial to the process of gathering and discharging samples.
According to another embodiment of the present invention, the notch of the guiding groove is not smaller than the nozzle of the first centrifugal tube, so as to completely receive the liquid flowing out of the first centrifugal tube.
According to another embodiment of the invention, the peripheral edge of the diversion trench is formed into a support ring, and the assembled connecting boss presses the support plate on the snap ring-shaped limiting structure through the support ring.
According to another embodiment of the invention, the flow guide is spherically shaped.
According to another embodiment of the present invention, the pumping hole is formed on and penetrates the snap ring-shaped stopper structure.
According to another embodiment of the invention, the second connecting seat is provided with a drainage platform, the drainage platform is positioned in the middle of the third connecting groove, and the lower end outlet of the catheter is positioned at the drainage platform.
According to another embodiment of the invention, the filter membrane is a microporous filter membrane and the support plate is a porous support plate.
According to another embodiment of the invention, the first centrifugal tube is in threaded connection with the first connecting groove, the connecting boss is in threaded connection with the second connecting groove, and the second centrifugal tube is in threaded connection with the third connecting groove.
The invention has the following beneficial effects:
according to the invention, the first centrifugal tube and the second centrifugal tube which are directly communicated are connected through the connecting assembly, the filtration is completed through the filtering assembly, the sample liquid only needs one transfer process, the structure is simplified, and the phenomenon of cross contamination is avoided.
The filter membrane adopts a compression type assembly structure, can be disassembled and replaced, can be suitable for suction filtration of samples for a long time due to a replaceable filter membrane structure, and is simple in structure and convenient to replace.
The present invention will be described in further detail with reference to the accompanying drawings.
Drawings
FIG. 1 is a schematic structural view of a centrifugal tube suction filtration device according to the present invention;
FIG. 2 is a schematic structural diagram of a first connecting seat according to the present invention;
FIG. 3 is a top view of FIG. 2;
FIG. 4 is a schematic view of a second connecting seat according to the present invention;
FIG. 5 is a top view of FIG. 4;
fig. 6 is a schematic structural view of the support plate of the present invention.
Detailed Description
In order that the above objects, features and advantages of the present invention can be more clearly understood, a more particular description of the invention will be rendered by reference to the appended drawings. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than those specifically described herein, and therefore the scope of the present invention is not limited to the specific embodiments disclosed below.
Example 1
This embodiment provides a centrifuging tube suction filtration device, as shown in fig. 1-6, it includes first centrifuging tube 100, second centrifuging tube 200, coupling assembling 300 and filter assembly 400, and wherein first centrifuging tube 100 is connected through coupling assembling 300 with second centrifuging tube 200, and filter assembly 400 assembles in coupling assembling 300.
Wherein the connection assembly 300 comprises a first connection seat 310 and a second connection seat 320 which are connected with each other, the first connection seat 310 is detachably connected with the first centrifugal tube 100 into a whole, and the second connection seat 320 is detachably connected with the second centrifugal tube 200;
be equipped with first connecting groove 311 and second connecting groove 312 on first connecting seat 310, be equipped with third connecting groove 321 and connection boss 322 on the second connecting seat 320, first centrifuging tube 100 threaded connection is in first connecting groove 311, connect boss 322 and can stretch into in the second connecting groove 312 and compress tightly fixed support plate, threaded connection between preferred connection boss 322 and the first connecting groove 311, second centrifuging tube 200 threaded connection is in third connecting groove 321.
One side of the first connection seat 310 is provided with a vent hole 313 communicated with the interior of the first centrifuge tube 100, the vent hole 313 is communicated with the atmosphere, one side of the second connection seat 320 is provided with a suction hole 323 communicated with the interior of the second centrifuge tube 200, a through liquid guide pipe 324 is arranged on the connection boss 322, the first centrifuge tube 100 is communicated with the interior of the second centrifuge tube 200 through a passage formed by the first connection groove 311, the second connection groove 312, the liquid guide pipe 324 on the connection boss 322 and the third connection groove 321, wherein the filter assembly 400 is arranged on the passage to perform filtration.
The filter assembly 400 comprises a filter membrane 410 and a support plate 420, wherein the filter membrane 410 is arranged on the support plate 420, for example, clamped on the support plate 420, and different use requirements can be met by replacing different types of filter membranes 410.
Wherein the filter membrane 410 is preferably a microporous filter membrane, and the support plate 420 is a porous support plate adapted to the microporous filter membrane for easy cleaning.
Further, in order to facilitate the compression of the support plate 420 and the filter membrane 410, the first connection groove 311 and the second connection groove 312 are shrunk to form a snap-ring-shaped limiting structure 314, and the assembled connection boss 322 compresses the support plate 420 on the snap-ring-shaped limiting structure 314, wherein the vent hole 313 is located on the snap-ring-shaped limiting structure 314 and penetrates through the snap-ring-shaped limiting structure 314, preferably arranged transversely, to communicate with the first centrifugal tube 100.
Still further, a diversion trench 325 is formed on the connection boss 322 in a downward concave manner, and the diversion trench 325 is preferably spherical in shape, so as to facilitate the process of gathering and discharging the sample.
Specifically, the notch of baffle 325 is configured to be no smaller than the mouth of first centrifuge tube 100 to completely receive the liquid flowing out of first centrifuge tube 100.
More specifically, the outer circumferential edge of the guiding groove 325 is formed as a support ring 326, and the assembled connecting boss 322 presses the support plate 420 against the snap ring-shaped limiting structure 314 through the support ring 326.
Wherein the second connecting seat 320 is provided with a drainage platform 327, the drainage platform 327 is located in the middle of the third connecting groove 321, the lower outlet of the liquid guide tube 324 is located at the drainage platform 327, and the filtered sample flows into the flow guide groove 325 and flows out from the drainage platform 327 through the liquid guide tube 324 to enter the second centrifuge tube 200.
The first centrifuge tube 100, the catheter 324 and the second centrifuge tube 200 are collinear to facilitate the collection and discharge of the sample.
The aspirating hole 323 of this embodiment links to each other with the vacuum pump, keeps the negative pressure form in the second centrifuging tube 200 through the vacuum pump of being connected with aspirating hole 323, and the sample that is located first centrifuging tube 100 passes from first connecting slot 311 outflow back, in filter membrane 410, guiding gutter 325 and catheter entering second centrifuging tube 200, has realized the suction filtration when carrying out the sample transfer.
Although the present invention has been described with reference to the preferred embodiments, it is not intended to limit the scope of the invention. It will be appreciated by those skilled in the art that changes may be made without departing from the scope of the invention, and it is intended that all matter contained in the above description or shown in the accompanying drawings shall be interpreted as illustrative and not in a limiting sense.
Claims (10)
1. A centrifuge tube suction filtration device, comprising:
a first centrifuge tube (100);
a second centrifuge tube (200);
a connection assembly (300) through which the first centrifuge tube (100) and the second centrifuge tube (200) are connected;
the connecting assembly (300) comprises a first connecting seat (310) and a second connecting seat (320) which are connected with each other, the first connecting seat (310) is detachably connected with the first centrifugal tube (100) into a whole, and the second connecting seat (320) is detachably connected with the second centrifugal tube (200);
and
a filter assembly (400);
the filter assembly (400) comprises a filter membrane (410) and a support plate (420), wherein the filter membrane (410) is arranged on the support plate (420);
the centrifuge tube comprises a first connecting seat (310), a second connecting seat (312), a third connecting seat (321) and a connecting boss (322), wherein the first connecting seat (310) is provided with the first connecting groove (311) and the second connecting groove (312), the first centrifuge tube (100) is connected in the first connecting groove (311), the connecting boss (322) can extend into the second connecting groove (312) and press and fix a supporting plate (420), and the second centrifuge tube (200) is connected in the third connecting groove (321);
wherein one side of the first connecting seat (310) is provided with a vent hole (313) communicated with the interior of the first centrifugal tube (100), one side of the second connecting seat (320) is provided with a suction hole (323) communicated with the interior of the second centrifugal tube (200), and the connecting boss (322) is provided with a through catheter (324);
the negative pressure state in the second centrifuge tube (200) is maintained by a vacuum pump connected with the suction hole (323), and the sample in the first centrifuge tube (100) passes through the filter membrane (410) and the catheter (324) to enter the second centrifuge tube (200).
2. The centrifuge tube suction filtration device according to claim 1, wherein the first connecting groove (311) and the second connecting groove (312) are shrunk to form a snap ring-shaped limiting structure (314), and the assembled connecting boss (322) presses the support plate (420) against the snap ring-shaped limiting structure (314).
3. The centrifuge tube suction filtration device according to claim 2, wherein the connection boss (322) is formed with a flow guide groove (325) by being recessed downward.
4. The centrifuge tube suction filtration apparatus of claim 3, wherein the notch of the diversion trench (325) is not smaller than the nozzle of the first centrifuge tube (100).
5. The centrifuge tube suction filtration device according to claim 3, wherein the peripheral edge of the guide groove (325) is formed as a support ring (326), and the assembled connection boss (322) presses the support plate (420) against the snap ring-shaped stopper structure (314) via the support ring (326).
6. The centrifuge tube suction filtration apparatus of claim 3, wherein the diversion trench (325) is spherical in shape.
7. The centrifuge tube suction filtration apparatus of claim 2, wherein the suction hole (323) is located on the snap ring-shaped stop structure (314) and penetrates the snap ring-shaped stop structure (314).
8. The centrifugal tube suction filtration device according to claim 3, wherein the second connecting seat (320) is provided with a drainage platform (327), the drainage platform (327) is located in the middle of the third connecting groove (321), and the lower end outlet of the liquid guide tube (324) is located at the drainage platform (327).
9. The centrifuge tube suction filtration apparatus of claim 1, wherein the filter membrane (410) is a microporous filter membrane and the support plate (420) is a porous support plate.
10. The centrifuge tube suction filtration device according to claim 1, wherein the first centrifuge tube (100) is screwed into the first connecting groove (311), the connecting boss (322) is screwed into the second connecting groove (312), and the second centrifuge tube (200) is screwed into the third connecting groove (321).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011537454.4A CN112717513B (en) | 2020-12-23 | 2020-12-23 | Centrifuging tube suction filter device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011537454.4A CN112717513B (en) | 2020-12-23 | 2020-12-23 | Centrifuging tube suction filter device |
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| Publication Number | Publication Date |
|---|---|
| CN112717513A true CN112717513A (en) | 2021-04-30 |
| CN112717513B CN112717513B (en) | 2023-03-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011537454.4A Active CN112717513B (en) | 2020-12-23 | 2020-12-23 | Centrifuging tube suction filter device |
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Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| EP2055385A1 (en) * | 2007-10-31 | 2009-05-06 | Roche Diagnostics GmbH | Method and device for purifying nucleic acids |
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| CN105879685A (en) * | 2016-05-30 | 2016-08-24 | 河海大学 | Vacuum filtration device for laboratory |
| CN106731091A (en) * | 2017-01-05 | 2017-05-31 | 上海安谱实验科技股份有限公司 | Vacuum apparatus |
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| CN108489767A (en) * | 2018-05-15 | 2018-09-04 | 华北水利水电大学 | A kind of the filtrate extraction element and method of filtering with microporous membrane |
| CN108854197A (en) * | 2018-07-25 | 2018-11-23 | 合肥宏立制冷科技有限公司 | A kind of broken steady type Vacuum filtration device of anti-filter paper |
| CN210186579U (en) * | 2019-04-22 | 2020-03-27 | 河南电池研究院有限公司 | Needle type filter device convenient for collecting sample liquid |
| CN210251415U (en) * | 2019-03-29 | 2020-04-07 | 浙江海洋大学 | Vacuum suction filtration device |
-
2020
- 2020-12-23 CN CN202011537454.4A patent/CN112717513B/en active Active
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE660878A (en) * | 1964-09-14 | 1965-09-10 | ||
| EP2055385A1 (en) * | 2007-10-31 | 2009-05-06 | Roche Diagnostics GmbH | Method and device for purifying nucleic acids |
| CN101791500A (en) * | 2010-02-11 | 2010-08-04 | 南京大学 | Drying and filtering integral device for organic extract liquid |
| CN202237453U (en) * | 2011-09-07 | 2012-05-30 | 耿益城 | Microfilter |
| CN103937662A (en) * | 2012-12-31 | 2014-07-23 | 江苏嘉语生物医药技术有限公司 | Microbe separating and culturing device and method |
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| CN112717513B (en) | 2023-03-03 |
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