CN112715939B - Preparation method of dihydromyricetin compound solution capable of being stably stored for long time - Google Patents
Preparation method of dihydromyricetin compound solution capable of being stably stored for long time Download PDFInfo
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- CN112715939B CN112715939B CN202011612608.1A CN202011612608A CN112715939B CN 112715939 B CN112715939 B CN 112715939B CN 202011612608 A CN202011612608 A CN 202011612608A CN 112715939 B CN112715939 B CN 112715939B
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- dihydromyricetin
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- ampelopsis grossedentata
- complex solution
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- KQILIWXGGKGKNX-UHFFFAOYSA-N dihydromyricetin Natural products OC1C(=C(Oc2cc(O)cc(O)c12)c3cc(O)c(O)c(O)c3)O KQILIWXGGKGKNX-UHFFFAOYSA-N 0.000 title claims abstract description 111
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- YWBYXMQTUULWSZ-UHFFFAOYSA-N ethanol 2-phenylchromen-4-one Chemical compound C(C)O.O1C(=CC(=O)C2=CC=CC=C12)C1=CC=CC=C1 YWBYXMQTUULWSZ-UHFFFAOYSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/66—Proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/03—Organic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
- A23L29/231—Pectin; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
- A23L29/262—Cellulose; Derivatives thereof, e.g. ethers
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
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- A—HUMAN NECESSITIES
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- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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Abstract
The invention discloses a preparation method of a dihydromyricetin compound solution capable of being stably stored for a long time, belonging to the technical field of production processes of functional foods or food raw materials. The particle size of the compound solution is 100 to 600nm, the dispersibility index is less than or equal to 0.3, the Zeta-potential is-10 to-40 mV, the compound solution has good physicochemical stability, the encapsulation rate of the dihydromyricetin is higher than 60 percent, and the loading amount of the dihydromyricetin exceeds 5mg/mL and inhibits the precipitation of the dihydromyricetin in the storage process.
Description
Technical Field
The invention relates to the technical field of production processes of functional foods or functional food raw materials, in particular to a preparation method of a dihydromyricetin compound solution capable of being stably stored for a long time.
Background
Dihydromyricetin is a flavonoid polyphenol with a plurality of physiological and pharmacological activities, the actions of the flavonoid polyphenol comprise antioxidation, anticancer, anti-inflammatory, antimicrobial, liver protection, lipid and glucose metabolism regulation, and the dihydromyricetin also has the actions of relieving alcoholism and protecting nerves, and ampelopsis grossedentata, hovenia dulcis thunb, grapes and berries are natural sources of the dihydromyricetin, wherein the content of the dihydromyricetin in the ampelopsis grossedentata is rich (more than 10 percent). Although the dihydromyricetin can be dissolved in hot water, the solubility is very low at low temperature or room temperature, and the functional beverage rich in the dihydromyricetin is easy to separate out and generate insoluble precipitate in the shelf life, which not only causes the deterioration of the appearance of the product, but also greatly influences the effective content of the dihydromyricetin. Therefore, the improvement of the solubility of the dihydromyricetin at low temperature or room temperature, in particular the long-term storage stability of the drink containing the dihydromyricetin, so as to improve the shelf life of the functional drink is a problem which needs to be solved for expanding the application of the dihydromyricetin in the field of functional foods.
Chinese patent 20151000453.8, the patent name "dihydromyricetin cyclodextrin inclusion compound and its preparation method", utilize cyclodextrin to coat dihydromyricetin, thus promote the water-solubility of dihydromyricetin. Although the scheme can improve the solubility of dihydromyricetin in water, the functional beverage is easy to separate out in shelf life after the coating product is added into the functional beverage.
Disclosure of Invention
Aiming at the problems that precipitation is easy to precipitate in the shelf life of functional beverage rich in dihydromyricetin and the long-term stability is not high, the invention aims to provide a preparation method of dihydromyricetin compound solution capable of being stably stored for a long time.
The invention provides the following technical scheme:
a method for preparing dihydromyricetin complex solution capable of being stably stored for a long time comprises the following steps:
(1) Diluting the ethanol solution of dihydromyricetin with water, adding gliadin, and stirring to obtain organic phase;
(2) Slowly adding the organic phase into sodium caseinate solution to form dihydromyricetin-protein complex solution, and evaporating ethanol to obtain dihydromyricetin-protein complex solution;
(3) Mixing the dioxymyricetin-protein complex with the anionic polysaccharide solution, and adjusting the pH value to be acidic to obtain a dihydromyricetin-protein-polysaccharide complex solution;
(4) Homogenizing dihydromyricetin-protein-polysaccharide complex solution under high pressure, and sterilizing to obtain the dihydromyricetin complex solution.
In the technical scheme of the invention, firstly, mixing grain alcohol-soluble protein and an ethanol solution containing dihydromyricetin to obtain an organic phase in which the grain alcohol-soluble protein and the dihydromyricetin are co-dissolved, forming primary embedding of a dihydromyricetin pair, adding the primary embedded dihydromyricetin into a sodium caseinate solution, and performing secondary coating on the dihydromyricetin; then, anionic polysaccharide with negative charges is introduced, the pH value is adjusted to be acidic, and the anionic polysaccharide is coated on the outer side of dihydromyricetin-cereal prolamin-sodium caseinate to form three-layer coating through electrostatic interaction between sodium caseinate and the anionic polysaccharide, so that the compound is endowed with very good dispersion stability in water through a three-layer coating system, and the long-term storage stability of functional drinks and health-care foods containing dihydromyricetin is improved. In addition, in the technical scheme of the application, the solvent is evaporated in the step (2), such as vacuum evaporation, and the recovered ethanol can be recycled.
Preferably, the ethanol solution of the dihydromyricetin is obtained by extracting dihydromyricetin in the ampelopsis grossedentata raw material by ethanol and then removing insoluble precipitates, wherein the content of the dihydromyricetin is 30-50 mg/mL.
Preferably, the ampelopsis grossedentata raw material comprises at least one of ampelopsis grossedentata, ampelopsis grossedentata powder, ampelopsis grossedentata extract, ampelopsis grossedentata flavone or ampelopsis grossedentata product rich in dihydromyricetin.
Both can adopt ampelopsis grossedentata extract and dihydromyricetin as the main material among the technical scheme of this application, also can directly extract dihydromyricetin and carry out normal position embedding from ampelopsis grossedentata, but directly adopt the ampelopsis grossedentata raw materials to extract and compare with the dihydromyricetin after the purification, the composition in the ampelopsis grossedentata is complicated, the technical degree of difficulty of dihydromyricetin embedding has been increased, also influence the instability of ampelopsis grossedentata beverage simultaneously, and the technical scheme of this application provides the scheme of the long-term stability of ampelopsis grossedentata drink of pertinence solution richness containing dihydromyricetin.
As preferred for the process of the present invention, the prolamins include, but are not limited to, zein, rice prolamin, hordein, wheat gliadin.
As the optimization of the method, the volume concentration of ethanol in the dihydromyricetin ethanol solution diluted by water in the step (1) is 70-85 percent; the mass concentration of the cereal prolamin is 1-5%.
Preferably, the mass concentration of the sodium caseinate solution used in the step (2) is 1-8%; the mass of the sodium caseinate and the cereal prolamin is 1.
Preferably, the anionic polysaccharide used in step (3) is one or more of sodium carboxymethylcellulose, pectin and soybean polysaccharide, or a compound colloid containing one or more of sodium carboxymethylcellulose, pectin and soybean polysaccharide; the mass ratio of the anionic polysaccharide to the sodium caseinate is 2-1.
Preferably, the method of the invention, the pH value is adjusted by using edible acidity regulator in the step (3); and (4) adjusting the pH value in the step (3) to be 3.5-4.5 at the end point. The edible acid can be selected from citric acid, lactic acid, malic acid, etc.
As the method of the present invention, the high-pressure homogenization is preferably a two-stage high-pressure homogenization at a pressure of 200/50MPa.
The invention has the following beneficial effects:
the preparation method of the invention obtains a compound solution containing dihydromyricetin, three layers of coatings of the dihydromyricetin are formed by utilizing anionic polysaccharide, sodium caseinate and cereal prolamin, the encapsulation rate of the dihydromyricetin reaches 60-90%, the content of the dihydromyricetin is high, more importantly, the particle size distribution of the compound in the obtained compound solution is uniform, the long-term storage stability is high, the compound solution can be used for preparing functional drinks or beverages, the precipitation of the dihydromyricetin in the low-temperature and normal-temperature storage processes is inhibited, the normal-temperature and low-temperature long-term storage stability of the functional drinks or health-care foods rich in the dihydromyricetin is improved, the precipitation is avoided, the shelf life is prolonged, the process is simple, the energy consumption is low, and the solvent can be recycled.
Drawings
FIG. 1 is a schematic diagram showing a method for preparing a dihydromyricetin complex solution of example 1.
FIG. 2 is a graph showing the particle size distribution of the dihydromyricetin complex solution prepared in example 1 before and after storage at room temperature.
FIG. 3 is a graph showing a comparison of the state of example 1, comparative example and dihydromyricetin aqueous solution after leaving standing under different conditions.
Detailed Description
The following further describes embodiments of the present invention.
The starting materials used in the present invention are commercially available or commonly used in the art, unless otherwise specified, and the methods in the following examples are conventional in the art, unless otherwise specified.
The encapsulation efficiency in each of the following examples and comparative examples was calculated according to the following formula: encapsulation ratio (%) = dihydromyricetin content in soluble portion in complex/total amount of dihydromyricetin added to prepare complex × 100%.
Example 1
A method for preparing dihydromyricetin complex solution capable of being stored stably for a long time is shown in figure 1, and the preparation process comprises: (1) Weighing 5g of ampelopsis grossedentata flavone, fully stirring and dissolving in 100mL of ethanol, and centrifuging for 30min at 4000rpm to remove insoluble precipitate to obtain a dihydromyricetin ethanol solution; adding water to dilute until the volume concentration of ethanol is 80% (v/v), taking 100mL, dissolving zein until the concentration is 4%, and fully stirring and dissolving to prepare an organic phase;
(2) Slowly adding 100mL of organic phase into 100mL of sodium caseinate solution with protein concentration of 4% to form dihydromyricetin-protein complex solution, evaporating at 35 deg.C for 45min by vacuum evaporation method to remove ethanol to obtain dihydromyricetin-protein complex, and recovering the evaporated ethanol;
(3) Adding water into the dihydromyricetin-protein complex until the total volume is 200mL, uniformly mixing, and centrifuging at 4000rpm for 30min to remove insoluble aggregates; then taking 200mL of centrifugal supernatant solution, adding a sodium carboxymethylcellulose solution with the mass concentration of 2% according to the volume ratio of 1;
(4) Homogenizing dihydromyricetin-protein-polysaccharide complex solution under high pressure of 200/50Mpa, and heating in 95 deg.C water bath for 10min for sterilization to obtain dihydromyricetin complex solution.
In the composite solution obtained after sterilization in step (4) in this example, the average particle size of the particles was 426nm, the dispersion index PDI was 0.248, the zeta potential was-42.9 mV, and the encapsulation efficiency of dihydromyricetin was 80.4%.
Example 2
This example differs from example 1 in that the protein content of the sodium caseinate solution was 6%, i.e. zein casein to sodium carboxymethylcellulose = 2.
In this example, the average particle diameter of the dihydromyricetin complex solution was 493nm, the dispersion index PDI was 0.263, the Zeta potential was-41.5 mV, and the dihydromyricetin encapsulation efficiency was 83.4%.
Example 3
The difference between this example and example 1 is that the concentration of the vine tea flavone ethanol solution is low, i.e. 2g vine tea flavone is weighed out and dissolved in 100mL ethanol by stirring, and other preparation steps are the same as example 1.
In this example, the average particle size of the dihydromyricetin complex solution was 475nm, the dispersion index PDI was 0.237, the Zeta potential was-42.2 mV, and the encapsulation efficiency of dihydromyricetin was 85.6%.
Example 4
This example is different from example 1 in that the anionic polysaccharide solution was a soybean polysaccharide solution having a mass concentration of 2%, and the other preparation steps were the same as example 1.
In this example, the average particle diameter of the dihydromyricetin complex solution was 144nm, the dispersion index PDI was 0.151, and the encapsulation efficiency of dihydromyricetin with a zeta potential of-10.3 mV was 80.6%.
Example 5
The difference between this example and example 1 is that the anionic polysaccharide solution is a pectin solution with a mass concentration of 2%, and the other preparation steps are the same as those in example 1.
In this example, the average particle size of the dihydromyricetin complex solution was 238nm, the dispersion index PDI was 0.196, and the entrapment rate of dihydromyricetin at a zeta potential of-26.2 mV was 82.3%.
Example 6
The difference between this example and example 1 is that the dihydromyricetin is derived from the ampelopsis grossedentata extract, the specific method is to dissolve 10g of ampelopsis grossedentata extract and ethanol, extract for 2h under stirring, then centrifuge under 4000rpm to remove insoluble impurities, the supernatant is dihydromyricetin ethanol solution, the anionic polysaccharide solution is soybean polysaccharide solution with mass concentration of 2%, and the rest of the preparation steps are the same as those in example 1.
In this example, the average particle diameter of the dihydromyricetin complex solution was 147nm, the dispersion index PDI was 0.139, the Zeta potential was-23.5 mV, and the encapsulation efficiency of dihydromyricetin was 61.2%.
Example 7
This example is different from example 1 in that dihydromyricetin is derived from ampelopsis grossedentata by grinding ampelopsis grossedentata into ampelopsis grossedentata powder, placing 25g of ampelopsis grossedentata powder into 100mL of ethanol, extracting for 2 hours under stirring, filtering the extract with a 200-mesh screen, centrifuging for 30 minutes at 4000rpm, removing insoluble impurities to obtain an ethanol solution of dihydromyricetin, and further, the anionic polysaccharide solution is a soybean polysaccharide solution having a mass concentration of 2%, and the remaining preparation steps are the same as those in example 1.
In this example, the average particle size of the dihydromyricetin solution was 223nm, the dispersion index PDI was 0.216, the Zeta potential was-22.7 mV, and the dihydromyricetin encapsulation efficiency was 79.5%.
Example 8
The difference between this example and example 1 is that the anionic polysaccharide solution is propylene glycol alginate with a mass concentration of 2%, and the other preparation steps are the same as those in example 1.
In this example, the average particle size of the dihydromyricetin solution was 1061nm, the dispersion index PDI was 0.411, the Zeta potential was-16.7 mV, and the dihydromyricetin encapsulation efficiency was 65.3%.
Comparative example
The difference from example 1 is that the third coating was not performed using anionic polysaccharide, pH was not adjusted, pH6.5 was maintained, and 0.5% nisin was added as a bacteriostatic agent to ensure that the bacterial cells were not affected by microorganisms during storage.
The solution of the composite obtained in the comparative example had a particle diameter of 109nm, PDI of 0.112 and a zeta potential of-31.1 mV.
Performance testing
1. The long-term storage property of the dihydromyricetin compound solution
1) The change in the particle size distribution of the dihydromyricetin complex solution prepared in example 1 after storage at room temperature for 3 months is shown in fig. 2, wherein the storage at room temperature is tested to be left standing at 25 ℃. As can be seen from the figure, the particle size distribution does not change greatly after being stored for 3 months at normal temperature, and the composite particles with large particle size (about 1000 nm) are relatively reduced, which probably is because the composite with larger particle size is precipitated in the process of storage and standing, and the whole particle size still maintains a narrow distribution range.
2) The dihydromyricetin compound solution prepared in example 1 and the dihydromyricetin aqueous solution prepared in the comparative example are respectively stored for three months under the condition of normal temperature illumination and three months under the condition of low temperature illumination, the precipitation of each solution is shown in figure 3, wherein the low temperature storage test is standing at 4 ℃; the dihydromyricetin water solution is dihydromyricetin extract solution of Ampelopsis grossedentata flavone, and the mass content of dihydromyricetin reaches 72.4%. And the prepared dihydromyricetin aqueous solution is an aqueous solution at the temperature of 90 ℃ due to the low solubility of the dihydromyricetin. As can be seen from the figure, only a very small amount of sediment was observed after three months of storage at low temperature, and the stability was maintained as a whole, compared to the immediately prepared composite solution and the comparative example.
2. Long-term storage Properties of Dihydromyricetin Complex solutions of the above examples and comparative examples
1) The dihydromyricetin complex solutions prepared in the above examples and comparative examples were stored at normal temperature for three months and at low temperature for three months, respectively, and then tested for PDI dispersion index and average particle size, and the results are shown in table 1.
TABLE 1 storage results of dihydromyricetin complex solutions of examples and comparative examples
As can be seen from the table above, in the compound solution containing dioxomyricetin prepared by the method of the invention, the encapsulation rate of dihydromyricetin is 60-90%, the average particle size of the compound is within the range of 100-600 nm, the dispersity index is less than 0.3, and the Zeta potential is-10-40 mV; and the polysaccharide has good stability after storage at normal temperature and low temperature, the dispersion index is not changed greatly, and meanwhile, in terms of selection of anionic polysaccharide, the sodium carboxymethylcellulose, pectin, soybean polysaccharide and the like have better effects than propylene glycol alginate, and have lower dispersion index, especially on long-term storage effect.
2) The dihydromyricetin complex solution and the pure dihydromyricetin aqueous solution prepared in the above examples and comparative examples were stored under normal temperature illumination for three months and at low temperature for three months, respectively, and then the soluble dihydromyricetin content was measured, and the results are shown in table 2 (corresponding to the samples in fig. 2).
TABLE 2 Dihydromyricetin content in solutions after storage under different conditions for each example and comparative example
In the above table, it can be seen that in the compound solution containing dioxymyricetin prepared by the method of the present invention, the content of soluble dihydromyricetin can reach 7mg/mL, and the loading amounts of different anionic polysaccharide encapsulated compounds are different, but no matter under the conditions of normal temperature illumination and low temperature storage, the loss rate of soluble dihydromyricetin in three months is far less than that of non-embedded dihydromyricetin.
Because the compound solution obtained by the preparation method is the water dispersion system of the dihydromyricetin, namely a phase-change functional drink, the functional drink prepared from the compound solution can reduce or eliminate the influence of the dihydromyricetin and improve the long-term storage stability of the functional drink.
Claims (7)
1. A method for preparing a dihydromyricetin compound solution capable of being stably stored for a long time is characterized by comprising the following steps:
(1) Diluting the ethanol solution of dihydromyricetin with water, adding gliadin, and stirring to obtain organic phase;
(2) Slowly adding the organic phase into the sodium caseinate solution to form a dihydromyricetin-protein complex solution, and evaporating ethanol to obtain a dihydromyricetin-protein complex solution;
(3) Mixing the dioxymyricetin-protein complex with the anionic polysaccharide solution, and adjusting the pH value to be acidic to obtain a dihydromyricetin-protein-polysaccharide complex solution;
(4) Homogenizing dihydromyricetin-protein-polysaccharide complex solution under high pressure, and sterilizing to obtain dihydromyricetin complex solution; the concentration of the dihydromyricetin in the ethanol solution of the dihydromyricetin in the step (1) is 30-50 mg/mL;
the volume concentration of ethanol in the dihydromyricetin ethanol solution after being diluted by water is 70-85 percent;
the mass concentration of the cereal prolamin is 1-5 percent;
the mass ratio of the sodium caseinate to the cereal prolamin is 1;
the anionic polysaccharide is one or more of sodium carboxymethylcellulose, pectin and soybean polysaccharide, or a compound colloid containing one or more of sodium carboxymethylcellulose, pectin and soybean polysaccharide;
the mass ratio of the anionic polysaccharide to the sodium caseinate is 2;
and (4) adjusting the pH value in the step (3) to be 3.5-4.5 at the end point.
2. A method for preparing a dihydromyricetin complex solution capable of being stored stably for a long period of time as claimed in claim 1, wherein said alcoholic solution of dihydromyricetin is obtained by extracting dihydromyricetin from Ampelopsis grossedentata raw material with ethanol, and removing insoluble precipitate.
3. A method for preparing a dihydromyricetin complex solution capable of being stored stably for a long period of time as claimed in claim 2, wherein the Ampelopsis grossedentata raw material comprises at least one of Ampelopsis grossedentata, ampelopsis grossedentata powder, ampelopsis grossedentata extract, ampelopsis grossedentata flavone or Ampelopsis grossedentata product rich in dihydromyricetin.
4. A method according to claim 1, wherein the prolamines include, but are not limited to, zein, rice prolamine, hordein, and triticale prolamine.
5. A method for preparing a dihydromyricetin complex solution capable of being stored stably for a long time according to claim 1, wherein the mass concentration of the sodium caseinate solution used in the step (2) is 1% to 8%.
6. A method for preparing a dihydromyricetin complex solution that can be stored stably for a long period of time according to claim 1, wherein the pH is adjusted using an edible acidity regulator in step (3).
7. A method for producing a dihydromyricetin complex solution that can be stored stably for a long period of time as claimed in claim 1, wherein the high-pressure homogenization is a two-stage high-pressure homogenization at a pressure of 200/50MPa.
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