CN112710655A - Method for sampling and detecting pesticide residues before mulberry leaves are picked and fed - Google Patents
Method for sampling and detecting pesticide residues before mulberry leaves are picked and fed Download PDFInfo
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
Abstract
The invention discloses a method for sampling and detecting pesticide residues before picking and feeding mulberry leaves, which relates to the technical field of silkworm breeding and comprises the steps of determining leaf positions of the mulberry leaves to be picked and sampled, and determining a sampling range according to partition planning of a mulberry field after determining the sampled leaf positions; edge sampling points are uniformly arranged at the edge part of a sampling range, the distance between every two edge sampling points is not more than 20 meters, middle sampling points are uniformly arranged within the edge of the sampling range, the distance between every two adjacent middle sampling points and the distance between the middle sampling points and the edge sampling points are not more than 50 meters, sampling is carried out at the determined edge sampling points and the middle sampling points, and pesticide residue detection is carried out on the sampled mulberry leaves by using a quick detection bag; the invention determines a targeted sampling method, avoids the defect of wide sampling, has convenient and fast detection of a sampling object and simple identification of a detection result, and is convenient for realizing the rapid sampling detection of pesticide residues on mulberry leaves.
Description
Technical Field
The invention relates to the technical field of silkworm breeding, in particular to a method for detecting pesticide residue in picked mulberry leaves before feeding silkworms.
Background
The silkworm industry in China has a history of more than five thousand years, is an indispensable composition of Chinese national culture, is an industry which makes important contribution to national economic growth, and the production quantity and trade quantity of Chinese silkworm cocoons and silks are still high in the top in the world at present. The large-scale intensive silkworm breeding mode which is vigorously developed by leading enterprises represented by silk companies with more than scale is started in each silkworm breeding area, but the silkworm breeding environment is gradually deteriorated due to the use of a large amount of chemical pesticides and the continuous change of natural environment and global climate while the silkworm industry scale is rapidly increased and the income of silkworm farmers is greatly improved, so that the invasion and erosion of profits of silkworm farmers and silk factories are gradually increased.
In recent years, due to the nonstandard use of pesticides, the influence of high-toxicity, long-acting and biological pesticides used by other crops around the mulberry field and the popularization of high-pressure and high-altitude pesticide spraying devices such as large-scale pesticide spraying machines, unmanned planes, large-scale planes and the like, the pesticide action time is greatly prolonged, and the action range is more dozens of times enlarged. Such as: the residual toxicity periods of dichlorvos, dimethoate, methyl parathion and the like are generally within 15 days, and the maximum residual toxicity period of the long-acting medicaments such as methamidophos, quinalphos, isocarbophos, fipronil and the like is more than 50 days; the pesticide is manually sprayed to plants by the sprayer, the height of the sprayed pesticide is consistent with that of the plants, the pesticide is generally limited in a crop garden, the influence range is generally not more than 100m, the pesticide sprayed at high altitude by an unmanned aerial vehicle and a large aircraft can drift to a far place along with wind, and if windy weather is met or the local terrain is a canyon zone, the wind blows in the same direction, and the pesticide can drift to a distance of about 30km along with the wind. The deterioration of silkworm breeding environment leads to that the work of preventing mulberry leaves from being polluted by pesticide in a mulberry field is more and more difficult to develop, the reports of pesticide poisoning conditions of silkworms in various silkworm areas are increased day by day, a large number of silkworms are rapidly killed when the concentration is high, and chronic poisoning of silkworms is caused when the concentration is low, so that the phenomena of no silking and no cocooning are caused, great economic loss is caused to silkworm farmers, and the enthusiasm of the silkworm farmers is greatly attacked. The pesticide poisoning phenomenon of silkworm is a problem of the sustainable development of health of the cocoon silk industry.
The research of silkworm researchers on silkworm poisoning mainly focuses on the characterization analysis and classification after silkworm poisoning, the treatment and mechanism change after drug detoxification and silkworm poisoning, and the prevention method and measure for silkworm poisoning do not change for decades, or the method and measure mainly pass through 1. communicate with units or farmers around a mulberry field, and are expected to give notice when the silkworm poisoning is taken and avoid the silkworm period as much as possible; 2. before picking mulberry leaves, patrolling a picking area, and observing whether the periphery is sprayed with pesticide and smells the pesticide taste; 3. after picking up mulberry leaves, some mulberry leaves are randomly selected for feeding, selected and rejected weak young silkworms and sleepers are tested for toxicity, and the like, so that the pesticide poisoning of the mulberry leaves of the silkworms is prevented, but the three methods have defects respectively. The method 1 is difficult to prevent the pesticide spraying action of a remote farmland or forestry department, and the method 2 and the method 3 have the defects of good response to the high-concentration pesticide and acute pesticide poisoning condition and incapability of reflecting the poisoning condition in time on the chronic poisoning condition caused by trace pesticide. The method and the instrument for detecting pesticide residues of samples such as fruits, vegetables and tea have the advantages of high sensitivity, small required amount of detected samples and the like, but also have the defects of high detection error rate, complex steps, professional detection training, high cost or long detection period, and cannot adapt to the silkworm breeding practice of low general culture degree of silkworm farmers, large labor intensity in silkworm breeding period, short interval time for feeding mulberry leaves in silkworm breeding period and the like.
In summary, the current method for detecting pesticide residues of mulberry leaves for silkworms is not applicable, and the importance of silkworm farmers in detecting pesticide residues of the mulberry leaves is not enough, so that most silkworm farmers and even a considerable part of silkworm farmers do not establish a perfect silkworm pesticide poisoning defense mechanism, whether silkworms are poisoned by pesticides is completely determined, and once the silkworm poisoning is caused by pesticide pollution of the mulberry leaves, the silkworm poisoning is disastrous. The phenomenon that mulberry leaves are polluted by pesticides to cause silkworm poisoning needs to be solved urgently.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, and provides a method for sampling and detecting pesticide residues before mulberry leaves are picked and fed, so as to solve the technical problem that the mulberry leaf pesticide residue detection method in the prior art has defects.
The invention is realized by the following technical scheme:
a method for sampling and detecting pesticide residues before mulberry leaves are picked and fed comprises sampling and detecting, wherein the sampling comprises the following specific steps:
step 1, determining leaf positions of mulberry leaves needing to be picked and sampled
Wherein, when the instar stage of the silkworm is the stage of collecting ants, the leaf position required to be fed is 2-3 leaves, and the color of the mulberry leaf is green and yellow; when the instar of the silkworm is 1, the leaf position required to be fed is 3-4 leaves, and the color of the mulberry leaves is changed to tender green; when the instar of the silkworm is 2, the leaf position required to be fed is 4-5 leaves, and the color of the mulberry leaves is just dark green; when the instar of the silkworm is 3, the leaves to be fed are 5-7 leaves, and the color of the mulberry leaves is dark green; when the silkworm is at the age of 4-5, the leaf of the dead core bud is used at the age of four; when the five ages are five years old, the mulberry leaves are bred by cutting and are dark green; determining specific sampling leaf positions according to the age of the silkworms needing to be fed;
step 2, determining a sampling range according to the partition planning of the mulberry field;
step 3, uniformly arranging edge sampling points at the edge part of the sampling range, wherein the distance between every two edge sampling points is not more than 20 meters, uniformly arranging middle sampling points within the edge of the sampling range, and the distances between every two adjacent middle sampling points and between the middle sampling point and the edge sampling point are not more than 50 meters;
step 4, sampling is carried out at the determined edge sampling point and the middle sampling point;
and 5, carrying out pesticide residue detection on the sampled mulberry leaves by using a rapid detection package.
Further, the rapid detection package is composed of a solvent A, a reagent B and a liquid taking tube, wherein the solvent A is composed of a reagent bottle packaged in a sealing way and a detection liquid filled in the reagent bottle, and the detection liquid is a buffer solution with the pH value of 8.0 prepared by dissolving 23.80g of anhydrous dipotassium hydrogen phosphate and 6.40g of potassium dihydrogen phosphate in 1000mL of distilled water;
the reagent A is a white tablet prepared from acetylcholinesterase, the reagent B is a red medicine prepared from indophenol acetate, and each of the reagent A and the reagent B is hermetically packaged by an independent aluminum film.
Further, the specific steps for detecting pesticide residues are as follows:
cutting mulberry leaves obtained by sampling, randomly taking two 200g sample fragments, fully soaking the two sample fragments in 500ml of purified water for 10min to obtain two soaking solutions, respectively taking 5ml of soaking solutions, simultaneously taking 5ml of purified water, respectively adding 5ml of solvent A into the three sample fragments by using a liquid taking pipe, respectively adding one part of reagent A, shaking, uniformly mixing, standing for 15min, respectively adding one part of reagent B, uniformly mixing, standing for 2-3min, performing color comparison on two mixed solutions obtained from the soaking solutions and a mixed solution obtained from the purified water, and judging pesticide residue.
Further, the sampling time is the time period with the lowest air temperature in one day such as morning or evening, and 2-3 pieces of mulberry leaves are sampled at each sampling point.
Further, step 5 is followed by:
and 6, immediately stopping picking the mulberry leaves in the sampling range of the sampling object in which the pesticide residue is detected, and selecting a mulberry field for picking.
Further, step 6 also comprises the steps of reserving the sample, and determining the pesticide type and concentration, thereby eliminating false positive caused by secondary substances contained in the sampling object, and determining the residual period according to the pesticide type, so as to recover the use of the mulberry leaves in the corresponding sampling range after the pesticide residual period, and reduce the waste of the mulberry leaves.
Further, the method also includes:
and 7, if no pesticide residue is detected, feeding some mulberry leaves obtained by sampling at random, and feeding a plurality of selected and eliminated weak young silkworms or bradynostalgias with the mulberry leaves obtained by sampling for toxin test.
Compared with the prior art, the invention has the following advantages:
1. the sampling method provided by the invention can effectively realize accurate sampling, the leaf positions required to be collected are determined according to the age of the silkworms required to be fed, and the leaf positions are sampled in a targeted manner, so that the carelessness of wide sampling is avoided, and the detection is more targeted;
2. aiming at the characteristics of pesticide spraying, different sampling points are arranged at the edge and the inner part of the sampling range, and the obtained sample has more representativeness;
3. according to the invention, the rapid detection can be realized by arranging the rapid detection packet, the detection result is easy to distinguish, the identification degree is improved, the operation difficulty of the detection is reduced, and the method is suitable for popularization and application;
4. according to the method, the mulberry field is planned to sample, so that on one hand, the representativeness of sampling is guaranteed, and on the other hand, the sampling workload is reduced.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
An embodiment of the invention is described below:
because the mulberry leaves are picked for feeding the silkworms, and the required leaf positions of the silkworms in different instars are different, before picking, the instars of the silkworms needing to be fed with the picked mulberry leaves need to be determined, so that the leaf positions of the mulberry leaves can be determined to meet the feeding requirements, and in the embodiment, the leaf positions required for sampling are determined according to table 1.
TABLE 1
Because the good mulberry is an important condition for feeding the silkworms, the good mulberry is the mulberry leaves which are ripe, have no insect eyes, no pesticide residue and have proper water content. The mature leaves refer to mulberry leaves which are respectively suitable for the nutritional requirements of the silkworms in each age, the silkworm varieties are generally 4-sleep five-year old, and the leaf positions of the mature leaves of the silkworms in the ages are shown in the corresponding table 1. The different leaves picked in the same mulberry field may have different pesticide residue detection results because the different kinds of pesticides have different action mechanisms and different residue times. Some pesticide residues are long, old leaves with low leaf positions are possibly polluted by pesticide, and newly grown tender leaves do not contain pesticide; some pesticides act on the ground to kill pests on the surface or roots of the mulberry, the mulberry leaves at the lower leaf positions have no residual pesticide, and the new tender leaves have trace pesticide because of the absorption and the transportation of the pesticide by the roots; and trace pesticide floating from a distance can pollute leaves at the upper part of the branch, and the leaves at the lower part are prevented from being polluted due to the obstruction of the leaves at the upper part. The mulberry leaves at the leaves with proper maturity are picked as the pesticide residue detection sample, so that the workload can be reduced compared with the whole sampling detection, and the situation that the whole mulberry leaves in the area are abandoned due to the detection of the pesticide residue can be avoided.
After the sampling leaf position is determined, the sampling range needs to be determined. Currently, with the development of the sericulture industry in a large-scale and intensive direction, the mulberry field is larger and larger, so that the mulberry fields of dozens of mu and hundreds of mu are very common, and the mulberry fields of thousands of mu are also commonly reported. The mulberry leaves yield per mu in the mulberry field in the mature period can reach 2500 kilograms per year, the middle and lower reaches of Yangtze river basically germinates and twigs in late March, and leaves fall after blooming for 11 months; 2.5 boxes of silkworm seeds can be fed in each mu of mulberry field, and the silkworm period from the harvesting of the ants to the clustering of the silkworms in each season is about 23 days. In order to improve the utilization rate of mulberry leaves in a mulberry field and the working efficiency of workers, silkworm households with large scales are fed in batches according to the reality, and the mulberry field is divided into regions for alternately picking and cutting, so that the benefit is maximized; namely, a mulberry field partition is made in advance, one partition corresponds to a batch of silkworms, and mulberry leaves required by the batch of silkworms are picked from the partition as much as possible. The mulberry leaf sampling range is determined according to early mulberry field partition planning, and sampling is carried out in an area where leaves are to be sampled, so that the consequence that detection conclusion is invalid due to unnecessary detection and sampling deviating from a leaf-picking area caused by full-coverage sampling of a mulberry field is avoided.
After the sampling range is defined, the sampling point is determined for sampling. Because the sampling points in the defined mulberry field are better, the workload is increased, and the detection cost is increased. The selection of sampling points is related to the reliability and accuracy of detection, the comprehensive representativeness of the sampling points is ensured while the sampling points are arranged as few as possible, the design principle is that the probability that the periphery of a picking area is polluted by pesticides is greater than the middle part of the picking area, at least 2 sampling points are preferably arranged on each side, and the distance between adjacent sampling points is not greater than 20 meters; at least 1-2 sampling points are preferably arranged in the middle of the picking area, and the distances between the middle sampling points and the middle sampling point and the nearest edge sampling point are not more than 50 meters; and thirdly, not less than 5-6 sampling points are arranged in one mu.
After the completion of the above condition confirmation, the sampling time and the number of times are confirmed. Picking mulberry leaves is generally carried out in the morning or evening at a time with lower air temperature in one day, the picking of samples is finished in half an hour before workers enter the garden to work, and 2-3 mulberry leaves are taken at each sampling point; the times are determined according to the mulberry leaf picking times, and the comprehensive sampling and detection of pesticide residues in a mulberry field are increased once before a batch of silkworm is raised.
Pesticide residue detection is carried out after sampling is finished
At present, agricultural and forestry use a great amount of pesticides for weeding, killing insects and preventing diseases, the types of the pesticides are as many as hundreds, but organophosphorus pesticides represented by chloramine phosphate, dimethoate, cartap, phoxim and the like account for about 50 percent of the total pesticide use amount due to the advantages of broad spectrum, high efficiency, low toxicity, easy degradation and the like, and among various pesticides, the organophosphorus pesticides are most researched on the toxicity mechanism and the drug resistance of silkworms and comprise a plurality of aspects of cell cycle regulatory factors, apoptosis, oxidative stress and the like. The organophosphorus pesticide mainly has competitive inhibition effect with insect acetylcholinesterase, so that acetylcholine can not be hydrolyzed in time at a receptor part, and accumulation of acetylcholine at the insect receptor part is caused, and finally insects are over excited and die due to damage of a nervous system. The pesticide poisoning incidents of silkworms caused by the three main classes of pesticides account for more than 70% of the total number of the silkworm poisoning incidents.
The determination method is based on the principle that organophosphorus and carbamate pesticides can inhibit the activity of cholinesterase, if the extract of mulberry leaves does not contain organophosphorus and carbamate pesticide residues or the residue is low, the activity of the enzyme is not inhibited, the substrate added in the test can be hydrolyzed by the enzyme, and the hydrolysate reacts with the added color developing agent to generate color or the hydrolysate has color. If the extract solution of mulberry leaves contains pesticides and has a high residue, the enzyme activity is inhibited, the matrix is not hydrolyzed, and no or little color change occurs when a color developer is added. The method comprises the following specific steps:
1) the sample is cut into 1 cm square pieces by scissors and mixed evenly, two 200g sample pieces are taken randomly and divided into sample 1 and sample 2, and each sample is fully soaked in 500ml of purified water for 10 min.
2) Respectively measuring 5.0mL of soak solution, adding 5.0mL of solvent A and one part of reagent A, oscillating, uniformly mixing, and standing for reaction for about 15 min; simultaneously measuring 5.0mL of purified water, adding 5.0mL of solvent A and one part of reagent A, shaking, uniformly mixing, standing and reacting for about 15min to serve as a blank control.
3) Adding a part of reagent B into each mixed solution, uniformly mixing, and standing for 2-3min to fully react.
4) Comparing the color of the two sample solutions with that of a blank control to judge whether pesticide residues exist or approximate residual concentration; comparisons should also be made between the two sample solutions to test the accuracy and reproducibility of the experiment.
3. And (5) verifying the result. Immediately stopping picking leaves in the mulberry field of the mulberry leaves with pesticide residues, and selecting another mulberry field for picking leaves; and a sample is also required to be reserved for further determination and determination of the pesticide type and concentration, so that false positive caused by secondary substances contained in the mulberry leaves is eliminated, the residual period can be determined according to the pesticide type, the use of the mulberry leaves in the mulberry field can be recovered as soon as possible after the pesticide residual period, and the mulberry leaf waste is reduced. If no pesticide residue is detected, some mulberry leaves are randomly fed to weak young silkworms and dormant silkworms which are selected and eliminated for virus testing to strengthen the prevention effect.
4. And (5) rapidly detecting the packet composition. The rapid detection package comprises a solution A, a reagent B and a liquid taking tube which are all 4 parts, and specifically comprises the following components:
1) the solution A was a buffer solution (prepared by dissolving 23.80g of anhydrous dipotassium hydrogen phosphate and 6.40g of potassium dihydrogen phosphate in 1000mL of distilled water and packaging) having a pH of 8.0, 200.0mL, and was sealed and packaged in a white plastic bottle.
2) The reagent A is acetylcholinesterase and 50 white tablets, each tablet is sealed and packaged by an independent aluminum film and is stored at low temperature of 4 ℃.
3) The reagent B is indophenol acetate and red tablets, the total number of the reagent B is 50, each tablet is hermetically packaged by an independent aluminum film, and the tablet is stored at a low temperature of 4 ℃. Acetylcholinesterase catalyzes indophenol acetate to produce acetic acid and blue indophenol.
4) 100 liquid taking tubes made of transparent plastic can roughly measure 5.0mL of solution.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (7)
1. A method for sampling and detecting pesticide residues before mulberry leaves are picked and fed is characterized by comprising sampling and detecting, wherein the sampling comprises the following specific steps:
step 1, determining leaf positions of mulberry leaves needing to be picked and sampled
Wherein, when the instar stage of the silkworm is the stage of collecting ants, the leaf position required to be fed is 2-3 leaves, and the color of the mulberry leaf is green and yellow; when the instar of the silkworm is 1, the leaf position required to be fed is 3-4 leaves, and the color of the mulberry leaves is changed to tender green; when the instar of the silkworm is 2, the leaf position required to be fed is 4-5 leaves, and the color of the mulberry leaves is just dark green; when the instar of the silkworm is 3, the leaves to be fed are 5-7 leaves, and the color of the mulberry leaves is dark green; when the silkworm is at the age of 4-5, the leaf of the dead core bud is used at the age of four; cutting mulberry for five years of age, and feeding mulberry leaves in dark green; determining specific sampling leaf positions according to the age of the silkworms needing to be fed;
step 2, determining a sampling range according to the partition planning of the mulberry field;
step 3, uniformly arranging edge sampling points at the edge part of the sampling range, wherein the distance between every two edge sampling points is not more than 20 meters, uniformly arranging middle sampling points within the edge of the sampling range, and the distances between every two adjacent middle sampling points and between the middle sampling point and the edge sampling point are not more than 50 meters;
step 4, sampling is carried out at the determined edge sampling point and the middle sampling point;
and 5, carrying out pesticide residue detection on the sampled mulberry leaves by using a rapid detection package.
2. The method for sampling and detecting pesticide residues before mulberry leaves are picked and fed according to claim 1, wherein the rapid detection package is composed of a solvent A, a reagent B and a liquid taking tube, wherein the solvent A is composed of a reagent bottle packaged in a sealing manner and a detection liquid contained in the reagent bottle, and the detection liquid is a buffer solution with pH of 8.0 prepared by dissolving 23.80g of anhydrous dipotassium hydrogen phosphate and 6.40g of potassium dihydrogen phosphate in 1000mL of distilled water;
the reagent A is a white tablet prepared from acetylcholinesterase, the reagent B is a red medicine prepared from indophenol acetate, and each of the reagent A and the reagent B is hermetically packaged by an independent aluminum film.
3. The method for sampling and detecting pesticide residues before mulberry leaves are picked and fed according to claim 2, wherein the pesticide residue detection is carried out by the following specific steps:
cutting mulberry leaves obtained by sampling, randomly taking two 200g sample fragments, fully soaking the two samples for 10min by using 500ml of purified water to obtain two soaking solutions, respectively taking 5ml of soaking solutions, simultaneously taking 5ml of purified water, respectively adding 5ml of solvent A into the three by using liquid taking pipes, respectively adding one part of reagent A, shaking, uniformly mixing, standing for 15min, respectively adding one part of reagent B, uniformly mixing, standing for 2-3min, performing color comparison on two mixed solutions obtained from the soaking solutions and a mixed solution obtained from the purified water, and judging pesticide residue.
4. The method for sampling and detecting pesticide residues before mulberry leaves are picked and fed as claimed in claim 3, wherein the sampling time is the time period with the lowest air temperature in one day such as morning or evening, and 2-3 mulberry leaves are taken at each sampling point.
5. The method for sampling and detecting pesticide residues before mulberry leaf picking and feeding as claimed in claim 4, further comprising the following steps after the step 5:
and 6, immediately stopping picking the mulberry leaves in the sampling range of the sampling object in which the pesticide residue is detected, and selecting a mulberry field for picking.
6. The method for sampling and detecting pesticide residues before mulberry leaves are picked and fed according to claim 5, wherein the step 6 further comprises the steps of reserving the samples, determining the types and concentrations of pesticides, thereby eliminating false positives caused by secondary substances contained in the sampling objects, and determining the residual period according to the types of the pesticides so as to recover the use of the mulberry leaves in the corresponding sampling range after the pesticide residual period, thereby reducing the waste of the mulberry leaves.
7. The method for detecting pesticide residues by sampling mulberry leaves before picking and feeding according to claim 6, further comprising the following steps:
and 7, if no pesticide residue is detected, randomly taking some mulberry leaves obtained by sampling to feed, and feeding a plurality of selected and eliminated weak young silkworms or bradynostalgias with the mulberry leaves obtained by sampling to test poison.
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| CN1687756A (en) * | 2004-12-27 | 2005-10-26 | 南京农业大学 | Kit for testing pesticide residue rapidly and use method thereof |
| JP2013145250A (en) * | 2013-04-25 | 2013-07-25 | Genome Soyaku Kenkyusho:Kk | Toxicity testing method |
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