CN112708548A - Intelligent tumor mutation load detection system and method based on 5G and block chain - Google Patents

Intelligent tumor mutation load detection system and method based on 5G and block chain Download PDF

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Publication number
CN112708548A
CN112708548A CN202110096742.9A CN202110096742A CN112708548A CN 112708548 A CN112708548 A CN 112708548A CN 202110096742 A CN202110096742 A CN 202110096742A CN 112708548 A CN112708548 A CN 112708548A
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fixedly connected
sliding
rotating
rod
plate
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CN202110096742.9A
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CN112708548B (en
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王亚东
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Xikang Software Co ltd
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Weifang Fengyuan Medical Technology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04Details of the conveyor system
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04Details of the conveyor system
    • G01N2035/0474Details of actuating means for conveyors or pipettes
    • G01N2035/0491Position sensing, encoding; closed-loop control

Abstract

The invention belongs to the technical field of intelligent tumor and gene detection, in particular to an intelligent tumor mutation load detection system and method based on 5G and a block chain, the scheme comprises a workbench, the inner wall of the bottom of the workbench is rotatably connected with a first rotating shaft and a second rotating shaft, the top end of the first rotating shaft extends to the top of the workbench and is fixedly connected with a first loading disc, the intelligent tumor mutation load detection system has a simple structure, a plurality of gene detection reagents can be clamped on the first loading disc at the same time, and the gene detection reagents are moved to the lower part of a detection probe through the intermittent rotation of the first loading disc and the second loading disc to detect the gene detection reagents, the detection process does not need manual operation, a large amount of gene detection reagents can be detected to obtain accurate data, and after the detection is finished, the gene detection reagents meeting the standard can be timely conveyed to a low-temperature storage tank to be stored, the information of the control panel is transmitted to a doctor work head table for recording and observation by using 5G and block chain technology.

Description

Intelligent tumor mutation load detection system and method based on 5G and block chain
Technical Field
The invention relates to the technical field of tumor and gene detection intelligence, in particular to a tumor mutation load detection intelligent system and method based on 5G and a block chain.
Background
Tumors are diseases caused by genomic variations. Immune checkpoint inhibitors open a new era in tumor therapy, but due to the lack of suitable clinical molecular markers, the benefited population of PD-1/PD-L1 (programmed cell death receptor-1, programmed death-1, PD-1; programmed cell death ligand-1, programmed cell death ligand1, PD-L1) drugs cannot be screened efficiently, with a screening rate of only 20% -30%. Tumor Mutation Burden (TMB) is an index that reflects the degree of total gene mutation in tumor cells, usually expressed as the total number of tumor somatic mutations contained per megabase (Mb) of tumor genomic region. Different types of tumors and different populations in the same tumor have different TMB levels, and in tumors with higher average TMB levels, the TMB levels of all patients are not high, and the proportion of populations with high TMB levels in different tumor categories is different, so that research shows that the high TMB levels can roughly predict the response probability of tumors such as lung cancer, bladder cancer, melanoma and the like to immune checkpoint inhibitor drugs. In view of the fact that TMB has achieved some good effects in clinical trials as a marker, large-scale companies or pharmaceutical enterprises have developed TMB biomarkers individually or cooperatively at home and abroad, and the detection range of the clinical trials of the immune checkpoint inhibitor drug is researched to incorporate TMB.
The 5G economy becomes one of the largest economies in the next years, is not only a new generation of mobile technology, but also a brand new network, and can effectively support and promote the development of the global artificial intelligence industry. Currently, the industry is steadily advancing the 4G to 5G transition. Once these network platforms are put into production, they will also provide necessary platform support for the medical internet of things and medical equipment, and the arrival of 5G network will promote the medical mode to a new height and bring significant economic benefits to the medical community, which can help users keep healthy through three abilities: medical internet of things (IoMT), enhanced mobile broadband (eMBB), and mission critical services. When the three are gathered together, the comprehensive and personalized service can be provided for the user at any time and any place.
In 2017, FDA approved Foundation OneCDx gene test kit adopts a single sample TMB measurement method, i.e., only cancer samples are sequenced, and then germline mutations are determined among gene mutations through a statistical method and information of a crowd database and filtered. This reduces the cost requirements of TMB detection to some extent. There are several drawbacks to this type of approach. First, the TMB varies widely between different cancer species, and thus a single sample does not detect sufficient accurate data and requires a large number of tests; secondly, the manual work of staff is required to detect in the current testing process, and this not only reduces detection efficiency but also increases staff's working strength, can't in time store the sample that the detection meets the demands after detecting in addition.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides an intelligent tumor mutation load detection system and method based on 5G and a block chain, which are compact in structure, do not need manual operation for detection, can detect large-scale samples and can store the samples in time.
In order to achieve the purpose, the invention adopts the following technical scheme:
tumor sudden change load detection intelligent system based on 5G and block chain, including the workstation, the bottom inner wall of workstation rotates and is connected with first pivot and second pivot, the top of first pivot extends to the top of workstation and the first loading dish of fixedly connected with, the top of second pivot extends to the top of workstation and fixedly connected with second loading dish, the top equidistance of first loading dish and second loading dish has arranged a plurality of standing grooves, the bottom inner wall fixedly connected with driving motor of workstation, driving motor's output shaft fixedly connected with disc, the skew one side fixedly connected with nose bar in the centre of a circle in the top of disc, the outer wall fixed cover of first pivot is equipped with intermittent type carousel, use first pivot as a plurality of recesses of equidistance of the annular equidistance of centre of a circle of arranging in the intermittent type carousel, the nose bar extends to in the recess, the outer walls of the first rotating shaft and the second rotating shaft are both fixedly sleeved with belt pulleys, the outer sides of the two belt pulleys are in transmission connection with the same belt, gene detection reagents are arranged in the placing grooves in the first loading disc, a clamping component for clamping the gene detection reagents is arranged in the first loading disc, the top of the workbench is fixedly connected with a fixed column, one side of the fixed column is provided with a detection component for detecting a gene detection reagent, the outer wall of the fixed column is connected with a sliding round sleeve in a sliding way, the top of the workbench is provided with a sliding component for enabling the sliding round sleeve to slide up and down, the top of slip circle cover rotates and is connected with the rotor plate, one side of slip circle cover is equipped with and is used for making the rotor plate rotate and the control assembly who resets, the top of rotor plate is equipped with the extraction element who is used for taking out gene detection reagent.
The centre gripping subassembly is including setting up the rectangular channel in first loading dish, the first spring of the equal fixedly connected with of both sides inner wall of rectangular channel, two the equal fixedly connected with slide bar in one side that first spring is close to each other, two the one end that the slide bar is close to each other all extends to in the standing groove and the first arc clamp splice of equal fixedly connected with, two one side that first arc clamp splice is close to each other all bumps with gene detection reagent mutually and touches, two the equal fixedly connected with connecting rod in one side of slide bar, two the equal fixedly connected with push rod in one side that the connecting rod is close to each other, the top sliding connection of first loading dish has the catch bar, the bottom of catch bar extends to the rectangular channel in and is located the top of push rod.
The detection assembly comprises a connecting plate fixedly connected to one side of the fixed column, the top of the connecting plate is fixedly connected with a rotating motor, an output shaft of the rotating motor is fixedly connected with a rotating disc, one side of the rotating disc, deviating from the circle center, is fixedly connected with a bump, the top of the connecting plate is slidably connected with a sliding rod, a second spring is sleeved on the outer wall of the sliding rod, the bottom end of the second spring is fixedly connected with the top of the connecting plate, the top end of the second spring is fixedly connected with the outer wall of the sliding rod, the bottom end of the sliding rod penetrates through the connecting plate and is fixedly connected with a detection probe, and the top end of the sliding rod is.
The sliding assembly comprises a cylinder fixedly connected to the top of the workbench, a piston rod of the cylinder is fixedly connected with a U-shaped plate, a rotating shaft is connected to the rotating column in a rotating mode, rotating rods are fixedly connected to the two ends of the rotating shaft, two are connected to the top end of each rotating rod and the bottom end of a sliding round sleeve in a sliding mode, two are connected to the bottom end of each rotating rod and the top portion of the workbench in a sliding mode, and the bottom ends of the rotating rods are all in contact with the U-shaped plate.
The control assembly comprises a stepping motor fixedly connected to one side of the sliding circular sleeve, an output shaft of the stepping motor is fixedly connected with a first gear, one side of a rotating plate is fixedly connected with a first rack, the first rack is meshed with the first gear, an arc-shaped groove is formed in the rotating plate, one side of the sliding circular sleeve is fixedly connected with a check block, one end, far away from the sliding circular sleeve, of the check block extends into the arc-shaped groove and is connected with the arc-shaped groove in a sliding mode, and one side of the inner wall of the arc-shaped groove is fixedly connected with an arc-shaped spring.
The extraction component comprises a small motor fixedly connected to the top of the rotating plate, an output shaft of the small motor is fixedly connected with a worm, the top of the rotating plate is rotatably connected with a second gear, a top circle center of the second gear is fixedly connected with a worm wheel, the worm wheel is meshed with the worm, the top of the rotating plate is slidably connected with two symmetrical sliding plates, two sliding plates are fixedly connected with a second arc-shaped clamping block on one side close to each other, two sliding plates are fixedly connected with a second rack on one side close to each other, and the second racks are meshed with the second gear.
Two equal fixedly connected with foam-rubber cushion in one side that first arc clamp splice is close to each other can prevent through the foam-rubber cushion that first arc clamp splice from pressing from both sides the damage with gene detection reagent.
The outer wall of the gene detection reagent is fixedly sleeved with a circular ring, and the gene detection reagent can be prevented from sliding down between the two second arc-shaped clamping blocks through the circular ring.
The top fixedly connected with fixture block of second pivot, the bottom centre of a circle that the second loaded the dish is equipped with the draw-in groove, the fixture block extends to in the draw-in groove, the top of workstation uses the second pivot to arrange a plurality of electric telescopic handle, a plurality of as centre of a circle annular equidistance electric telescopic handle of having, electric telescopic handle's the bottom all loads the bottom sliding connection who coils with the second, the top fixedly connected with pneumatic cylinder of workstation, the piston rod and the second of pneumatic cylinder load the dish and touch mutually, the top fixedly connected with low temperature storage case of workstation, one side fixed mounting of workstation has control panel, control panel and electric telescopic handle, driving motor, rotation motor, small motor, pneumatic cylinder, cylinder and step motor electric connection.
The detection method of the intelligent tumor mutation load detection system based on the 5G and the block chain comprises the following steps:
s1, starting a driving motor to drive the disc to rotate, enabling the convex rod to rotate along with the rotation of the disc to enter the groove, enabling the convex rod to rotate along with the rotation of the disc to drive the circular ring and the intermittent rotary disc to rotate intermittently, and enabling the first rotary shaft to drive the second loading disc and the second rotary shaft to rotate intermittently due to the fact that the circular ring and the second rotary shaft are connected through belt transmission, and enabling the gene detection reagent to rotate to the position right below the detection probe;
s2, starting a rotating motor to drive a rotating disc to rotate, wherein the rotating disc drives a lug to rotate, when the lug and a sliding rod are in contact, the lug pushes the sliding rod and a detection probe downwards, a second spring starts to compress, the detection probe enters a gene detection reagent downwards for detection, after the detection is finished, the lug continues to rotate, and after the lug is separated from the contact with the sliding rod, the sliding rod and the detection probe slide upwards under the action of the elastic force of the second spring, so that the detection probe is reset;
s3, when a detection sample meets the requirement, starting an air cylinder, wherein a piston rod of the air cylinder contracts to drive a U-shaped plate to contract, a rotating rod loses the blockage of the U-shaped plate and cannot support a sliding round sleeve, the rotating rod starts to rotate by taking a rotating shaft as a fulcrum, the sliding round sleeve, a rotating plate and a sliding plate move downwards under the action of gravity, the rotating plate moves downwards and pushes a push rod downwards, the push rod slides downwards to push two push rods towards two sides, a first spring starts to compress, the two slide rods and a first arc-shaped clamping block can slide towards two sides, and the clamping of the first arc-shaped clamping block on a gene detection reagent is released;
s4, starting a small motor to drive a worm to rotate, wherein the worm is meshed with a worm wheel, a second gear is meshed with a second rack, two sliding plates slide towards the middle along with the rotation of the second gear and the worm wheel, so that a gene detection reagent can be clamped, starting an air cylinder, and extending a piston rod of the air cylinder, so that a sliding round sleeve, a rotating plate and the sliding plates are pushed upwards by a rotating rod, and the gene detection reagent is separated from a placing groove;
s5, starting the stepping motor to drive the first gear to rotate, wherein the first gear is meshed with the first rack, the first gear drives the first rack, the rotating plate, the arc spring and the sliding plate to rotate 180 degrees, the arc spring is in contact with the stop block, the stop block extrudes the arc spring, then the air cylinder is started, a piston rod of the air cylinder, the sliding round sleeve, the rotating plate and the sliding plate descend to enable the gene detection reagent to fall into the placing groove on the second loading disc, the small motor is started, the small motor drives the worm to rotate reversely, clamping of the sliding plate on the gene detection reagent is released, then the sliding plate slides upwards to prevent the sliding plate from touching the gene detection reagent when the sliding plate rotates, and finally the rotating plate reversely rotates under the action of the arc spring to enable the rotating plate to reset;
and S6, when the gene detection reagent on the second loading disc is full, starting the electric telescopic rod, ejecting the second loading disc upwards by the electric telescopic rod, separating the clamping block from the clamping groove, releasing the clamping block from braking the clamping groove, starting the hydraulic cylinder, and pushing the second loading disc into the low-temperature storage box by the piston rod of the hydraulic cylinder for storage.
The invention has the following advantages:
1. the starting driving motor drives the disc to rotate, and the grooves are distributed on the intermittent turntable, so that the disc and the convex rod drive the first rotating shaft and the second rotating shaft to rotate intermittently through the belt, and the gene detection reagent can be accurately rotated to the position under the detection probe.
2. The telescopic cylinder piston rod can control the support of the rotating rod to the sliding round sleeve, and then the sliding round sleeve and the rotating plate can be adjusted to move up and down according to the situation.
3. The rotating plate slides downwards and extrudes the pushing rod downwards, the pushing rod slides downwards to enable the two pushing rods and the two sides of the first arc-shaped clamping block to slide, and therefore clamping of the first arc-shaped clamping block on the gene detection reagent can be relieved.
4. Step motor drive first gear revolve, and first gear can drive rotor plate and arc spring and rotate 180 degrees to carry the gene detect reagent of sliding plate centre gripping to the gene detect reagent on the second loading dish, after carrying, because before the arc spring with the dog touch mutually and cause the arc spring compression, after losing step motor's power, the elasticity of arc spring can drive rotor plate antiport, make the rotor plate reset.
5. The electric telescopic rod is started, the second loading disc can be ejected upwards by the electric telescopic rod, and the control of the clamping groove by the clamping block is removed, so that the piston rod of the hydraulic cylinder can convey the second loading disc into the low-temperature storage box, and a plurality of gene detection reagents are stored.
6. The outer wall of the gene detection reagent is provided with a circular ring, and the gene detection reagent can be prevented from sliding down from the second arc-shaped clamping block of the screw rod through the circular ring.
The invention has simple structure, can clamp a plurality of gene detection reagents on the first loading disc at the same time, move the gene detection reagents to the lower part of the detection probe through the intermittent rotation of the first loading disc and the second loading disc, detect the gene detection reagents, do not need manual operation in the detection process, can detect a large amount of gene detection reagents to obtain accurate data, can timely convey the gene detection reagents meeting the standard to a low-temperature storage box for storage after the detection is finished, transmit the information of the control panel to the corresponding block chain link points, store the received information data by the block chain link points, access the information data called from the block chain link points by the work head table in a 5G communication mode, and then doctors can conveniently record and observe the information from the work head table.
Description of the figures and
FIG. 1 is a front view of an intelligent system for detecting tumor mutation load based on 5G and a block chain, which is provided by the invention;
FIG. 2 is a front cross-sectional view of a workbench of the intelligent tumor mutation load detection system based on 5G and a block chain, which is provided by the invention;
FIG. 3 is a top view of a first loading tray of the intelligent tumor mutation load detection system based on 5G and a block chain according to the present invention;
FIG. 4 is a top cross-sectional view of a rectangular groove of the intelligent tumor mutation load detection system based on 5G and a block chain, which is provided by the invention;
FIG. 5 is a front cross-sectional view of a first arc-shaped clamping block of the intelligent tumor sudden change load detection system based on 5G and a block chain, which is provided by the invention;
FIG. 6 is a front cross-sectional view of a rectangular groove of the intelligent tumor mutation load detection system based on 5G and a block chain, which is provided by the invention;
FIG. 7 is an enlarged view of the intelligent system for tumor mutation load detection based on 5G and block chains according to the present invention at A;
FIG. 8 is a side view of the lifting assembly of the intelligent tumor mutation load detection system based on 5G and block chains according to the present invention;
FIG. 9 is a top view of the lifting assembly of the intelligent tumor mutation load detection system based on 5G and block chains according to the present invention;
FIG. 10 is a front cross-sectional view of the detection component of the intelligent tumor mutation load detection system based on 5G and a block chain according to the present invention;
FIG. 11 is a partial top cross-sectional view of a rotating plate of the intelligent tumor sudden change load detection system based on 5G and a block chain according to the present invention;
FIG. 12 is a front cross-sectional view of a sliding sleeve of the intelligent tumor mutation load detection system based on 5G and a block chain according to the present invention;
FIG. 13 is a top view of a disk and an intermittent rotary disk of the intelligent tumor mutation load detection system based on 5G and a block chain;
FIG. 14 is a block diagram of an intelligent system for tumor mutation load detection based on 5G and a block chain according to the present invention;
FIG. 15 is a front view of an intelligent system for tumor mutation load detection based on 5G and block chains in the second embodiment;
fig. 16 is a front sectional view of a second loading tray in the second embodiment;
fig. 17 is a bottom view of the latch and the catch groove in the second embodiment.
In the figure: 1. a work table; 2. a first loading tray; 3. a second loading tray; 4. a first rotating shaft; 5. a second rotating shaft; 6. a drive motor; 7. a disc; 8. a nose bar; 9. a circular ring; 10. an intermittent rotary table; 11. a groove; 12. a belt pulley; 13. a belt; 14. a rectangular groove; 15. a first spring; 16. a slide bar; 17. a connecting rod; 18. a push rod; 19. a push rod; 20. a placement groove; 21. a first arc-shaped clamping block; 22. a gene detection reagent; 23. fixing a column; 24. a connecting plate; 25. rotating the motor; 26. rotating the disc; 27. a bump; 28. a slide bar; 29. a second spring; 30. sliding the round sleeve; 31. a rotating shaft; 32. rotating the rod; 33. a U-shaped plate; 34. a cylinder; 35. a rotating plate; 36. a first rack; 37. a stepping motor; 38. a first gear; 39. an arc-shaped slot; 40. an arc-shaped spring; 41. a stopper; 42. a sliding plate; 43. a second arc-shaped clamping block; 44. a second gear; 45. a second rack; 46. a worm gear; 47. a small-sized motor; 48. a worm; 49. a clamping block; 50. a card slot; 51. an electric telescopic rod; 52. a hydraulic cylinder; 53. a low temperature storage tank; 54. a sponge cushion; 55. detecting the probe; 56. a control panel.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example one
Referring to fig. 1-14, an intelligent system for detecting tumor sudden-change load based on 5G and a block chain comprises a workbench 1, a first rotating shaft 4 and a second rotating shaft 5 are rotatably connected to the inner wall of the bottom of the workbench 1, the top end of the first rotating shaft 4 extends to the top of the workbench 1 and is fixedly connected with a first loading disc 2, the top end of the second rotating shaft 5 extends to the top of the workbench 1 and is fixedly connected with a second loading disc 3, a plurality of placing grooves 20 are equidistantly arranged on the tops of the first loading disc 2 and the second loading disc 3, a driving motor 6 is fixedly connected to the inner wall of the bottom of the workbench 1, a disc 7 is fixedly connected to an output shaft of the driving motor 6, a convex rod 8 is fixedly connected to one side of the top of the disc 7 deviating from the center of the circle, an intermittent rotating disc 10 is fixedly arranged on the outer wall of the first rotating shaft 4, a plurality of grooves 11 are annularly arranged in the intermittent rotating, protruding pole 8 extends to in the recess 11, the equal fixed cover of outer wall of first pivot 4 and second pivot 5 is equipped with belt pulley 12, the outside transmission of two belt pulleys 12 is connected with same belt 13, all be equipped with gene detection reagent 22 in the standing groove 20 in the first load dish 2, be equipped with the centre gripping subassembly that is used for the centre gripping of gene detection reagent 22 in the first load dish 2, the top fixedly connected with fixed column 23 of workstation 1, one side of fixed column 23 is equipped with the determine module that is used for detecting gene detection reagent 22, the outer wall sliding connection of fixed column 23 has slip round cover 30, the top of workstation 1 is equipped with the slip subassembly that is used for making slip round cover 30 gliding from top to bottom, the top of slip round cover 30 rotates and is connected with rotor plate 35, one side of slip round cover 30 is equipped with the control assembly that is used for making rotor plate 35 rotate and reset, the top of rotor plate 35 is equipped with the extraction component that is used for taking out.
According to the invention, the clamping assembly comprises a rectangular groove 14 arranged in the first loading disc 2, the inner walls of two sides of the rectangular groove 14 are fixedly connected with first springs 15, the sides, close to each other, of the two first springs 15 are fixedly connected with sliding rods 16, the ends, close to each other, of the two sliding rods 16 extend into a placing groove 20 and are fixedly connected with first arc-shaped clamping blocks 21, the sides, close to each other, of the two first arc-shaped clamping blocks 21 are in contact with a gene detection reagent 22, one sides, close to each other, of the two sliding rods 16 are fixedly connected with connecting rods 17, one sides, close to each other, of the two connecting rods 17 are fixedly connected with push rods 18, the top of the first loading disc 2 is slidably connected with a push rod 19, and the bottom end of the push rod 19 extends into the rectangular groove 14.
In the invention, the detection assembly comprises a connecting plate 24 fixedly connected to one side of a fixed column 23, the top of the connecting plate 24 is fixedly connected with a rotating motor 25, an output shaft of the rotating motor 25 is fixedly connected with a rotating disc 26, one side of the rotating disc 26 deviating from the center of a circle is fixedly connected with a bump 27, the top of the connecting plate 24 is slidably connected with a sliding rod 28, the outer wall of the sliding rod 28 is sleeved with a second spring 29, the bottom end of the second spring 29 is fixedly connected with the top of the connecting plate 24, the top end of the second spring 29 is fixedly connected with the outer wall of the sliding rod 28, the bottom end of the sliding rod 28 penetrates through the connecting plate 24 and is fixedly connected with a detection probe 55.
In the invention, the sliding assembly comprises an air cylinder 34 fixedly connected to the top of the workbench 1, a piston rod of the air cylinder 34 is fixedly connected with a U-shaped plate 33, a rotating shaft 31 is rotatably connected to the fixed column 23, two ends of the rotating shaft 31 are fixedly connected with rotating rods 32, the top ends of the two rotating rods 32 are both slidably connected with the bottom end of the sliding round sleeve 30, the bottom ends of the two rotating rods 32 are both slidably connected with the top of the workbench 1, and the bottom ends of the two rotating rods 32 are both contacted with the U-shaped plate 33.
In the invention, the control assembly comprises a stepping motor 37 fixedly connected to one side of the sliding circular sleeve 30, an output shaft of the stepping motor 37 is fixedly connected with a first gear 38, one side of the rotating plate 35 is fixedly connected with a first rack 36, the first rack 36 is meshed with the first gear 38, an arc-shaped groove 39 is arranged in the rotating plate 35, one side of the sliding circular sleeve 30 is fixedly connected with a stop block 41, one end, far away from the sliding circular sleeve 30, of the stop block 41 extends into the arc-shaped groove 39 and is in sliding connection with the arc-shaped groove 39, and the inner wall of one side of the arc-shaped groove 39 is fixedly connected with an arc.
According to the invention, the extraction assembly comprises a small motor 47 fixedly connected to the top of the rotating plate 35, an output shaft of the small motor 47 is fixedly connected with a worm 48, the top of the rotating plate 35 is rotatably connected with a second gear 44, the top circle center of the second gear 44 is fixedly connected with a worm gear 46, the worm gear 46 is meshed with the worm 48, the top of the rotating plate 35 is slidably connected with two symmetrical sliding plates 42, one sides of the two sliding plates 42 close to each other are fixedly connected with a second arc-shaped clamping block 43, one sides of the two sliding plates 42 close to each other are fixedly connected with a second rack 45, and the two second racks 45 are meshed with the second gear 44.
In the invention, the sponge pads 54 are fixedly connected to the mutually adjacent sides of the two first arc-shaped clamping blocks 21, and the first arc-shaped clamping blocks 21 can be prevented from clamping the gene detection reagent 22 by the sponge pads 54.
In the invention, the outer walls of the gene detection reagents 22 are fixedly sleeved with the rings 9, and the gene detection reagents 22 can be prevented from sliding off the two second arc-shaped clamping blocks 43 through the rings 9.
The detection method of the intelligent tumor mutation load detection system based on 5G and a block chain comprises the following steps:
s1, starting the driving motor 6 to drive the disc 7 to rotate, enabling the convex rod 8 to rotate to enter the groove 11 along with the rotation of the disc 7, enabling the convex rod 8 to drive the circular ring 9 and the intermittent rotary disc 10 to rotate intermittently along with the rotation of the disc 7, and enabling the circular ring 9 and the second rotary shaft 5 to be in transmission connection through the belt 13, so that the first rotary shaft 4 drives the second loading disc 3 and the second rotary shaft 5 to rotate intermittently, and the gene detection reagent 22 is rotated to be right below the detection probe 55;
s2, starting the rotating motor 25 to drive the rotating disc 26 to rotate, the rotating disc 26 driving the projection 27 to rotate, when the projection 27 and the sliding rod 28 touch, the projection 27 pushing the sliding rod 28 and the detection probe 55 downwards, the second spring 29 starting to compress, the detection probe 55 entering the gene detection reagent 22 downwards for detection, after the detection, the projection 27 continuing to rotate, after the projection 27 separating from the touch with the sliding rod 28, the sliding rod 28 and the detection probe 55 sliding upwards under the action of the elastic force of the second spring 29, and further the detection probe 55 resetting;
s3, when the detection sample meets the requirement, starting the air cylinder 34, wherein the piston rod of the air cylinder 34 contracts to drive the U-shaped plate 33 to contract, the rotating rod 32 loses the blockage of the U-shaped plate 33 and cannot support the sliding round sleeve 30, the rotating rod 32 starts to rotate by taking the rotating shaft 31 as a fulcrum, the sliding round sleeve 30, the rotating plate 35 and the sliding plate 42 move downwards under the action of gravity, the rotating plate 35 moves downwards and pushes the pushing rod 19 downwards, the pushing rod 19 slides downwards to push the two push rods 18 to two sides, the first spring 15 starts to compress, so that the two slide rods 16 and the first arc-shaped clamping block 21 can slide to two sides, and the clamping of the first arc-shaped clamping block 21 on the gene detection reagent 22 is released;
s4, starting a small-sized motor 47 to drive a worm 48 to rotate, wherein the worm 48 is meshed with a worm wheel 46, a second gear 44 is meshed with a second rack 45, two sliding plates 42 slide towards the middle along with the rotation of the second gear 44 and the worm wheel 46, and then the gene detection reagent 22 can be clamped, starting an air cylinder 34, and extending a piston rod of the air cylinder 34, so that a rotating rod 32 pushes a sliding round sleeve 30, a rotating plate 35 and the sliding plates 42 upwards, and the gene detection reagent 22 is separated from a placing groove 20;
s5, then the step motor 37 is started to drive the first gear 38 to rotate, the first gear 38 is engaged with the first rack 36, the first gear 38 drives the first rack 36, the rotating plate 35, the arc spring 40 and the sliding plate 42 to rotate 180 degrees, the arc spring 40 contacts with the stop 41, the stop 41 presses the arc spring 40, then, the cylinder 34 is actuated, the piston rod of the cylinder 34, the sliding sleeve 30, the rotating plate 35 and the sliding plate 42 are lowered, the gene assaying reagent 22 is dropped into the placing groove 20 on the second loading tray 3, the small motor 47 is started, the small motor 47 drives the worm 48 to rotate reversely, the clamping of the sliding plate 42 to the gene detection reagent 22 is released, then the sliding plate 42 slides upwards to prevent the sliding plate 42 from touching the gene detection reagent 22 when rotating, and finally the rotating plate 35 rotates reversely under the action of the arc-shaped spring 40 to reset the rotating plate 35;
s6, when the gene testing reagent 22 on the second loading tray 3 is full, the electric telescopic rod 51 is started, the electric telescopic rod 51 pushes the second loading tray 3 upward, the latch 49 is disengaged from the slot 50, the latch 49 releases the brake of the latch 50, and then the hydraulic cylinder 52 is started, and the piston rod of the hydraulic cylinder 52 pushes the second loading tray 3 into the low temperature storage tank 53 for storage.
Example two: as shown in fig. 15-17, the intelligent system for tumor mutation load detection based on 5G and block chains in this embodiment is different from the first embodiment in that: the top fixedly connected with fixture block 49 of second pivot 5, the bottom centre of a circle of second loading dish 3 is equipped with draw-in groove 50, fixture block 49 extends to in the draw-in groove 50, the top of workstation 1 uses second pivot 5 as centre of a circle annular equidistance a plurality of electric telescopic handle 51 of having arranged, the bottom of a plurality of electric telescopic handle 51 all with the bottom sliding connection of second loading dish 3, the top fixedly connected with pneumatic cylinder 52 of workstation 1, the piston rod of pneumatic cylinder 52 touches with second loading dish 3, the top fixedly connected with low temperature storage tank 53 of workstation 1, one side fixed mounting of workstation 1 has control panel 56, control panel 56 and electric telescopic handle 51, driving motor 6, rotating motor 25, small motor 47, pneumatic cylinder 52, cylinder 34 and step motor 37 electric connection.
However, as is well known to those skilled in the art, the working principle and wiring method of the control panel 56, the driving motor 6, the air cylinder 34, the rotating motor 25, the stepping motor 37, the small motor 47, the electric telescopic rod 51 and the hydraulic cylinder 52 are common and are conventional means or common knowledge, and will not be described herein, and those skilled in the art can make any choice according to their needs or convenience.
The working principle is as follows: firstly, the driving motor 6 is started to drive the disc 7 to rotate, the convex rod 8 rotates to enter the groove 11 along with the rotation of the disc 7, the convex rod 8 rotates along with the rotation of the disc 7 to drive the circular ring 9 and the intermittent rotary disk 10 to rotate intermittently, because the circular ring 9 and the second rotary shaft 5 are in transmission connection through the belt 13, the first rotary shaft 4 drives the second loading disk 3 and the second rotary shaft 5 to rotate intermittently, the gene detection reagent 22 rotates to be right below the detection probe 55, secondly, the rotating motor 25 is started to drive the rotary disk 26 to rotate, the rotary disk 26 drives the lug 27 to rotate, when the lug 27 and the sliding rod 28 touch, the lug 27 pushes the sliding rod 28 and the detection probe 55 downwards, the second spring 29 starts to compress, the detection probe 55 enters the gene detection reagent 22 downwards for detection, after the detection, the lug 27 continues to rotate, and after the lug 27 is separated from the touch of the sliding rod 28, the sliding rod 28 and the detecting probe 55 slide upwards under the action of the elastic force of the second spring 29, and reset, and in the third step, when the detected sample meets the requirement, the air cylinder 34 is started, the piston rod of the air cylinder 34 contracts to drive the U-shaped plate 33 to contract, the rotating rod 32 cannot support the sliding round sleeve 30 after losing the block of the U-shaped plate 33, the rotating rod 32 starts to rotate with the rotating shaft 31 as a fulcrum, the sliding round sleeve 30, the rotating plate 35 and the sliding plate 42 move downwards under the action of gravity, the rotating plate 35 moves downwards and pushes the pushing rod 19 downwards, the pushing rod 19 slides downwards to push the two pushing rods 18 to both sides, the first spring 15 starts to compress, and then the two sliding rods 16 and the first arc-shaped clamping block 21 can slide to both sides to release the clamping of the gene detecting reagent 22 by the first arc-shaped clamping block 21, in the fourth step, the small motor 47 is started to drive the worm 48 to rotate, and the worm 48 is meshed with the, the second gear 44 is engaged with the second rack 45, and the two sliding plates 42 slide toward the middle with the rotation of the second gear 44 and the worm gear 46, so as to clamp the gene detection reagent 22, then the cylinder 34 is started, the piston rod of the cylinder 34 is extended, so that the rotating rod 32 pushes the sliding sleeve 30, the rotating plate 35 and the sliding plates 42 upwards, so that the gene detection reagent 22 is separated from the placing groove 20, the fifth step is started, then the stepping motor 37 is started to drive the first gear 38 to rotate, the first gear 38 is engaged with the first rack 36, the first gear 38 drives the first rack 36, the rotating plate 35, the arc spring 40 and the sliding plates 42 to rotate 180 degrees, the arc spring 40 is contacted with the stopper 41, the stopper 41 presses the arc spring 40, then the cylinder 34 is started, the piston rod of the cylinder 34, the sliding sleeve 30, the rotating plate 35 and the sliding plates 42 descend, so that the gene detection reagent 22 falls into the placing groove 20 on the second loading tray 3, starting the small motor 47, the small motor 47 drives the worm 48 to rotate reversely, the sliding plate 42 is released from clamping the gene detection reagent 22, then the sliding plate 42 slides upwards to prevent the sliding plate 42 from touching the gene detection reagent 22 when rotating, finally the rotating plate 35 rotates reversely under the action of the arc spring 40 to reset the rotating plate 35, the sixth step is that after the gene detection reagent 22 on the second loading disc 3 is full, the electric telescopic rod 51 is started, the electric telescopic rod 51 pushes the second loading disc 3 upwards, the fixture block 49 is separated from the fixture groove 50, the brake of the fixture block 49 on the fixture groove 50 is released, then the hydraulic cylinder 52 is started, the piston rod of the hydraulic cylinder 52 pushes the second loading disc 3 into the low-temperature storage tank 53 for storage, the information of the control panel 56 is transmitted to the corresponding block link point, the block link point stores the received information data, the work head office accesses and retrieves the information data from the block link point by adopting a 5G communication mode, the doctor can record and observe the information data from the work head table conveniently and quickly.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (10)

1. Tumor sudden change load detection intelligent system based on 5G and block chain, including workstation (1), its characterized in that, the bottom inner wall of workstation (1) rotates and is connected with first pivot (4) and second pivot (5), the top of first pivot (4) extends to the top of workstation (1) and the first loading dish of fixedly connected with (2), the top of second pivot (5) extends to the top of workstation (1) and fixedly connected with second loading dish (3), a plurality of standing grooves (20) have been arranged to the top of first loading dish (2) and second loading dish (3) equal equidistance, the bottom inner wall fixedly connected with driving motor (6) of workstation (1), the output shaft fixedly connected with disc (7) of driving motor (6), one side fixedly connected with protruding pole (8) of the skew centre of a circle in the top of disc (7), the outer wall of the first rotating shaft (4) is fixedly sleeved with an intermittent rotating disc (10), a plurality of grooves (11) are annularly and equidistantly arranged in the intermittent rotating disc (10) by taking the first rotating shaft (4) as a circle center, the protruding rods (8) extend into the grooves (11), belt pulleys (12) are fixedly sleeved on the outer walls of the first rotating shaft (4) and the second rotating shaft (5), the two belt pulleys (12) are in transmission connection with the same belt (13) at the outer sides, gene detection reagents (22) are arranged in the placing grooves (20) in the first loading disc (2), clamping components for clamping the gene detection reagents (22) are arranged in the first loading disc (2), fixed fixing columns (23) are fixedly connected to the top of the workbench (1), and detection components for detecting the gene detection reagents (22) are arranged on one sides of the fixing columns (23), the outer wall sliding connection of fixed column (23) has slip circle cover (30), the top of workstation (1) is equipped with and is used for making slip circle cover (30) gliding slip subassembly from top to bottom, the top of slip circle cover (30) is rotated and is connected with rotor plate (35), one side of slip circle cover (30) is equipped with and is used for making rotor plate (35) rotate and the control assembly who resets, the top of rotor plate (35) is equipped with the extraction element who is used for taking out gene detection reagent (22).
2. The intelligent tumor sudden change load detection system based on 5G and a block chain as claimed in claim 1, wherein the clamping assembly comprises a rectangular groove (14) arranged in the first loading plate (2), the inner walls of both sides of the rectangular groove (14) are fixedly connected with first springs (15), one side of each of the two first springs (15) close to each other is fixedly connected with a sliding rod (16), one end of each of the two sliding rods (16) close to each other extends into the placing groove (20) and is fixedly connected with a first arc-shaped clamping block (21), one side of each of the two first arc-shaped clamping blocks (21) close to each other is contacted with a gene detection reagent (22), one side of each of the two sliding rods (16) is fixedly connected with a connecting rod (17), one side of each of the two connecting rods (17) close to each other is fixedly connected with a push rod (18), the top of the first loading disc (2) is connected with a pushing rod (19) in a sliding mode, and the bottom end of the pushing rod (19) extends into the rectangular groove (14) and is located above the pushing rod (18).
3. The intelligent tumor sudden change load detection system based on 5G and block chains as claimed in claim 1, wherein the detection assembly comprises a connecting plate (24) fixedly connected to one side of a fixed column (23), a rotating motor (25) is fixedly connected to the top of the connecting plate (24), a rotating disc (26) is fixedly connected to the output shaft of the rotating motor (25), a projection (27) is fixedly connected to one side of the rotating disc (26) deviating from the center of circle, a sliding rod (28) is slidably connected to the top of the connecting plate (24), a second spring (29) is sleeved on the outer wall of the sliding rod (28), the bottom end of the second spring (29) is fixedly connected to the top of the connecting plate (24), the top end of the second spring (29) is fixedly connected to the outer wall of the sliding rod (28), and the bottom end of the sliding rod (28) penetrates through the connecting plate (24) and is fixedly connected to a detection probe (55), the top end of the sliding rod (28) is contacted with the lug (27).
4. The intelligent system for detecting tumor sudden change load based on 5G and a block chain as claimed in claim 1, wherein the sliding assembly comprises a cylinder (34) fixedly connected to the top of the workbench (1), a piston rod of the cylinder (34) is fixedly connected with a U-shaped plate (33), a rotating shaft (31) is rotatably connected to the fixed column (23), two rotating shafts (32) are fixedly connected to two ends of the rotating shaft (31), two top ends of the rotating shafts (32) are slidably connected to the bottom end of the sliding circular sleeve (30), two bottom ends of the rotating shafts (32) are slidably connected to the top of the workbench (1), and two bottom ends of the rotating shafts (32) are in contact with the U-shaped plate (33).
5. The intelligent tumor sudden-change load detection system based on the 5G and block chain as claimed in claim 1, wherein the control assembly comprises a stepping motor (37) fixedly connected to one side of a sliding circular sleeve (30), an output shaft of the stepping motor (37) is fixedly connected with a first gear (38), one side of a rotating plate (35) is fixedly connected with a first rack (36), the first rack (36) is meshed with the first gear (38), an arc-shaped groove (39) is formed in the rotating plate (35), a stop block (41) is fixedly connected to one side of the sliding circular sleeve (30), one end, far away from the sliding circular sleeve (30), of the stop block (41 extends into the arc-shaped groove (39) and is in sliding connection with the arc-shaped groove (39), and an arc-shaped spring (40) is fixedly connected to the inner wall of one side of the arc-shaped groove (39).
6. The intelligent system for tumor mutation load detection based on 5G and block chains according to claim 1, it is characterized in that the extraction component comprises a small motor (47) fixedly connected with the top of the rotating plate (35), an output shaft of the small motor (47) is fixedly connected with a worm (48), the top of the rotating plate (35) is rotatably connected with a second gear (44), the top circle center of the second gear (44) is fixedly connected with a worm wheel (46), the worm wheel (46) is meshed with a worm (48), the top of the rotating plate (35) is connected with two symmetrical sliding plates (42) in a sliding mode, one sides, close to each other, of the two sliding plates (42) are fixedly connected with second arc-shaped clamping blocks (43), one sides, close to each other, of the two sliding plates (42) are fixedly connected with second racks (45), and the two second racks (45) are meshed with a second gear (44).
7. The intelligent tumor sudden change load detection system based on 5G and block chains as claimed in claim 2, wherein a sponge pad (54) is fixedly connected to one side of each of the two first arc-shaped clamping blocks (21) close to each other.
8. The intelligent system for tumor mutation burden detection based on 5G and block chains as claimed in claim 1, wherein the outer walls of a plurality of gene detection reagents (22) are fixedly sleeved with circular rings (9).
9. The intelligent tumor sudden change load detection system based on the 5G and the block chain as claimed in claim 1, wherein a clamping block (49) is fixedly connected to the top of the second rotating shaft (5), a clamping groove (50) is formed in the center of the bottom of the second loading disc (3), the clamping block (49) extends into the clamping groove (50), a plurality of electric telescopic rods (51) are annularly and equidistantly arranged on the top of the workbench (1) by taking the second rotating shaft (5) as the center of the circle, the bottom ends of the electric telescopic rods (51) are slidably connected with the bottom of the second loading disc (3), a hydraulic cylinder (52) is fixedly connected to the top of the workbench (1), a piston rod of the hydraulic cylinder (52) is in contact with the second loading disc (3), a low-temperature storage box (53) is fixedly connected to the top of the workbench (1), and a control panel (56) is fixedly mounted on one side of the workbench (1), the control panel (56) is electrically connected with the electric telescopic rod (51), the driving motor (6), the rotating motor (25), the small motor (47), the hydraulic cylinder (52), the air cylinder (34) and the stepping motor (37).
10. The detection method of the intelligent tumor mutation load detection system based on 5G and the block chain according to any one of claims 1 to 9, which is characterized in that: it comprises the following steps:
s1, starting a driving motor (6) to drive a disc (7) to rotate, rotating a convex rod (8) along with the rotation of the disc (7) to enter a groove (11), and driving a ring (9) and an intermittent rotary disc (10) to rotate intermittently along with the rotation of the disc (7), wherein the ring (9) is in transmission connection with a second rotary shaft (5) through a belt (13), so that the first rotary shaft (4) drives a second loading disc (3) and the second rotary shaft (5) to rotate intermittently, and a gene detection reagent (22) is rotated to be under a detection probe (55);
s2, starting a rotating motor (25) to drive a rotating disc (26) to rotate, wherein the rotating disc (26) drives a lug (27) to rotate, when the lug (27) and a sliding rod (28) touch, the sliding rod (28) and a detection probe (55) are pushed downwards by the lug (27), a second spring (29) starts to compress, the detection probe (55) enters a gene detection reagent (22) downwards for detection, after the detection is finished, the lug (27) continues to rotate, and after the lug (27) is separated from the touch with the sliding rod (28), the sliding rod (28) and the detection probe (55) slide upwards under the elastic force action of the second spring (29), so that the detection probe (55) is reset;
s3, when a detection sample meets the requirement, starting an air cylinder (34), wherein a piston rod of the air cylinder (34) contracts to drive a U-shaped plate (33) to contract, a rotating rod (32) cannot support a sliding round sleeve (30) after being blocked by the U-shaped plate (33), the rotating rod (32) starts to rotate by taking a rotating shaft (31) as a fulcrum, the sliding round sleeve (30), the rotating plate (35) and a sliding plate (42) move downwards under the action of gravity, the rotating plate (35) moves downwards and pushes a push rod (19) downwards, the push rod (19) slides downwards to push two push rods (18) to two sides, a first spring (15) starts to compress, and then the two sliding rods (16) and a first arc-shaped clamping block (21) can slide to two sides, so that the first arc-shaped clamping block (21) can release the clamping of the gene detection reagent (22);
s4, starting a small motor (47) to drive a worm (48) to rotate, wherein the worm (48) is meshed with a worm wheel (46), a second gear (44) is meshed with a second rack (45), two sliding plates (42) slide towards the middle along with the rotation of the second gear (44) and the worm wheel (46), and further the gene detection reagent (22) can be clamped, then starting an air cylinder (34), and extending a piston rod of the air cylinder (34), so that a rotating rod (32) pushes a sliding round sleeve (30), a rotating plate (35) and the sliding plates (42) upwards, and the gene detection reagent (22) is separated from a placing groove (20);
s5, then, a stepping motor (37) is started to drive a first gear (38) to rotate, the first gear (38) is meshed with a first rack (36), the first gear (38) drives the first rack (36), a rotating plate (35), an arc-shaped spring (40) and a sliding plate (42) to rotate 180 degrees, the arc-shaped spring (40) is contacted with a stop block (41), the stop block (41) extrudes the arc-shaped spring (40), then, an air cylinder (34) is started, a piston rod of the air cylinder (34) is started, a sliding round sleeve (30), the rotating plate (35) and the sliding plate (42) descend to enable a gene detection reagent (22) to fall in a placing groove (20) on a second loading disc (3), a small motor (47) is started, a worm (48) is driven by the small motor (47) to rotate reversely, clamping of the sliding plate (42) on the gene detection reagent (22) is released, then, the sliding plate (42) slides upwards, the sliding plate (42) is prevented from touching the gene detection reagent (22) when rotating, and finally the rotating plate (35) rotates reversely under the action of the arc-shaped spring (40) to reset the rotating plate (35);
s6, when the gene detection reagent (22) on the second loading disc (3) is full, starting the electric telescopic rod (51), the electric telescopic rod (51) pushes the second loading disc (3) upwards, the clamping block (49) is separated from the clamping groove (50), braking of the clamping block (49) on the clamping groove (50) is relieved, then starting the hydraulic cylinder (52), and the piston rod of the hydraulic cylinder (52) pushes the second loading disc (3) into the low-temperature storage tank (53) for storage.
CN202110096742.9A 2021-01-25 2021-01-25 Intelligent tumor mutation load detection system and method based on 5G and block chain Active CN112708548B (en)

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