CN112707595B - Hemp water deodorization method - Google Patents

Hemp water deodorization method Download PDF

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Publication number
CN112707595B
CN112707595B CN202011561086.7A CN202011561086A CN112707595B CN 112707595 B CN112707595 B CN 112707595B CN 202011561086 A CN202011561086 A CN 202011561086A CN 112707595 B CN112707595 B CN 112707595B
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hemp
water
stock solution
alkalized
culture
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CN112707595A (en
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陈涛
金刚
彭欣怡
黄显雅
覃旭
吴密
杨祥燕
刘明
陆勇
陈庆南
晏洪钟
崔明勇
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Guangxi Subtropical Crops Research Institute
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Guangxi Subtropical Crops Research Institute
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F9/00—Multistage treatment of water, waste water or sewage
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36—Adaptation or attenuation of cells
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/30—Nature of the water, waste water, sewage or sludge to be treated from the textile industry
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/02—Odour removal or prevention of malodour
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

Abstract

The invention provides a method for deodorizing hemp water, which comprises the following steps: s1, filtering hemp water, and removing hemp residues; s2, alkalizing the hemp water after removing the hemp residues through an alkaline filler to obtain alkalized hemp water; and S3, adding the alkalized hemp water into a mixed microbial inoculum, and fermenting for 5-10 days to obtain the deodorized hemp water. The method can effectively remove the odor in the hemp water, thereby effectively recycling the hemp water.

Description

Hemp water deodorization method
Technical Field
The invention relates to the technical field of hemp water treatment, in particular to a hemp water deodorization method.
Background
Sisal fiber, also known as sisal, has the characteristics of long fiber, pure white color, tough texture, high elasticity, strong tensile force, wear resistance and the like, can be used for manufacturing various sisal products such as slivers, ropes, polishing wheels, carpets, wadding mats, sisal wallpaper, steel wire rope cores, paper currency, artware and the like, and is widely applied to the fields of fishery, navigation, industrial and mining, transportation, oil fields and the like.
Sisal hemp fiber is obtained by scraping hemp, wherein the scraping is to scrape a peel shell (green peel) outside a fresh peel, hemp residue fiber and hemp water can be obtained after the scraping, the common hemp scraping procedure is to recover the hemp residue fiber and discard the hemp water, but the hemp water has stronger acidity and stronger acid odor, and is directly discarded to easily pollute the environment, and in addition, the hemp water contains rich nutrient substances, so that if the hemp water can be effectively recycled, the environmental pollution can be favorably reduced, and the investment of fertilizer can be reduced.
Disclosure of Invention
The invention provides a method for deodorizing hemp water, which can effectively remove the odor in the hemp water, thereby effectively recycling the hemp water.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a method for deodorizing hemp water comprises the following steps:
s1, filtering hemp water, and removing hemp residues;
s2, alkalizing the hemp water after removing the hemp residues through an alkaline filler to obtain alkalized hemp water;
and S3, adding the alkalized hemp water into a mixed microbial inoculum, and fermenting for 5-10 days to obtain the deodorized hemp water.
Further, the preparation method of the alkaline filler comprises the following steps:
A1. soaking sisal fiber in calcium hydroxide solution, adding stabilizer, chelating agent and binder, stirring, and freeze drying; obtaining the alkalized sisal fiber;
A2. placing the ceramsite into a colloidal solution of sodium carboxymethylcellulose, stirring, taking out, placing into a calcium hydroxide saturated solution at normal temperature, slowly heating at the speed of 0.5-1 ℃/min, keeping the temperature for 10-30 min when the temperature is raised to 90-99 ℃, taking out the ceramsite, and drying to obtain the alkalized ceramsite;
A3. taking a silicate aqueous solution with the modulus of 1.5-2.0, mixing the silicate aqueous solution with water according to the mass ratio of 0.5-1.2: 0.5-2, putting the mixture, the alkalized sisal fiber and the alkalized ceramsite into a mold, blowing nitrogen to fill for 5-10 min, and freeze-drying to obtain the alkaline filler.
Further, in the step a1, the stabilizer is vitamin C or ethylenediaminetetraacetic acid, and the chelating agent is disodium ethylenediaminetetraacetate, sodium citrate, trisodium hydroxyethylethylenediaminetriacetate; the binder is water-soluble cellulose or polyacrylamide.
Furthermore, the mass of the stabilizer is 0.001-0.1% of that of the sisal fiber, and the mass of the chelating agent is 0.1-0.8% of that of the calcium hydroxide.
Furthermore, the sisal fibers, the ceramsite and the silicate water solution are mixed according to the weight part ratio of 16-20: 40-55: 20-30.
Further, in step S3, the method for preparing the mixed bacterial agent includes the following steps:
B1. inoculating streptomyces griseus, bacillus mojavensis, bacillus pumilus and micromonospora in a mass ratio of 1-2: 2-3: 1 into a liquid culture medium, adding 0.1-0.5 wt% of tween into the liquid culture medium, culturing for 48-96 h, and when the number of composite viable bacteria reaches 1 x 10 8 When the concentration is more than CFU/mL, finishing the culture to obtain an activated strain stock solution;
B2. diluting the sesame water by 20-30 times, adding a liquid culture medium with the volume of 2-5 times of the sesame water, and culturing by 48-96 timesh, when the number of bacteria is more than 1 × 10 7 When the concentration is more than CFU/mL, finishing the culture to obtain a magma thallus stock solution;
B3. mixing the magma thallus stock solution and the activated strain stock solution according to the mass ratio of 1: 50-100, carrying out shake culture at 25-28 ℃ for 10-18 h, then carrying out normal-temperature culture for 24-72 h, and when the number of composite viable bacteria reaches 1 x 10 8 When the concentration is more than CFU/mL, obtaining primary culture strain stock solution, continuously mixing with the activated strain stock solution for gradual expansion culture to obtain deodorant strain stock solution, and drying the deodorant strain stock solution at low temperature to obtain the mixed microbial inoculum.
Further, the liquid medium contains the following components: 30-40 g/L glucose, 1.5-3 mg/L ammonium chloride, 300-400 mg/L calcium chloride, 100-200 mg/L inositol, 500-900 mg/L monopotassium phosphate, 200-300 mg/L magnesium sulfate, 15-20 mg/L ferrous sulfate, 5-10 mg/L manganese sulfate, 2-5 mg/L zinc sulfate, 0.2-0.5 mg/L sodium molybdate and 0.02-0.05 mg/L copper sulfate, and regulating the pH to 5-6 by using boric acid and acetic acid.
The invention also provides the application of the deodorized hemp water in liquid fertilizer.
The method for deodorizing the hemp water comprises the steps of removing hemp residues, adjusting the hemp water to weak acidity with pH of about 5.0-6.5 through an alkaline filler, fermenting with a mixed microbial inoculum, further improving the pH, inhibiting the activity of harmful bacteria in the hemp water, and degrading peculiar smell generated by self fermentation of the hemp water, so that the aim of deodorizing the hemp water is fulfilled.
Furthermore, the alkaline filler is adopted to adjust the pH value of the hemp water, the alkaline filler can be filled in processing equipment and can be used repeatedly for a long time without adding an alkaline neutralizer one by one, and the added calcium hydroxide reacts with the hemp water to generate calcium ions which can be used as a fertilizer. According to the method, the sisal fibers are firstly alkalized by calcium hydroxide, so that the toughness of the sisal fibers can be improved, the chelating agent can be used for slowly releasing calcium ions, meanwhile, the calcium hydroxide is loaded in the ceramsite, the aim of slowly releasing the calcium ions is achieved by the adsorption effect of the ceramsite, the calcium hydroxide and the ceramsite are mixed and bonded by a silicate aqueous solution, so that the alkaline filler with a microporous structure is formed, the hemp water penetrates through the filler, the alkaline substance is slowly released, the service cycle of the alkaline filler is longer, and the long-acting and lasting pH adjusting effect on the hemp water is ensured.
In addition, the streptomyces griseus, bacillus mojavensis, bacillus pumilus and micromonospora are mixed and fermented to generate enzyme for inhibiting the harmful bacteria in the hemp water, so that the growth of beneficial microorganisms is promoted, the rot of the hemp water is well inhibited, and the aim of deodorization is fulfilled.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
The present invention is further illustrated by the following specific examples, but the scope of the present invention is not limited to the following examples.
Example 1
A method for deodorizing hemp water comprises the following steps:
s1, filtering hemp water, and removing hemp residues;
s2, alkalizing the hemp water after removing the hemp residues through an alkaline filler to obtain alkalized hemp water;
and S3, adding the mixed microbial inoculum into the alkalized hemp liquid, and fermenting for 10 days to obtain the deodorized hemp liquid.
The preparation method of the alkaline filler comprises the following steps:
A1. soaking sisal fibers in a saturated calcium hydroxide solution, adding ethylenediaminetetraacetic acid, disodium ethylenediaminetetraacetate and polyacrylamide, wherein the mass of the ethylenediaminetetraacetic acid is 0.05% of that of the sisal fibers, and the mass of the disodium ethylenediaminetetraacetate is 0.2% of that of the calcium hydroxide, stirring, and freeze-drying to obtain the alkalized sisal fibers; A2. placing the ceramsite into a colloidal solution of sodium carboxymethylcellulose, wherein the mass fraction of the sodium carboxymethylcellulose is 1%, stirring, taking out, placing into a calcium hydroxide saturated solution at normal temperature, slowly heating at a speed of 0.5 ℃/min, keeping the temperature for 20min when the temperature is raised to 95 ℃, taking out the ceramsite, and drying to obtain the alkalized ceramsite; A3. taking a silicate aqueous solution with the modulus of 1.8, mixing the silicate aqueous solution with water according to the mass ratio of 1:1, putting the mixture, the alkalized sisal fiber and the alkalized ceramsite into a mold together, mixing the sisal fiber, the ceramsite and the silicate aqueous solution according to the weight ratio of 18:45:25, blowing nitrogen gas for filling for 5min, and carrying out freeze drying to obtain the alkaline filler.
In step S3, the method for preparing the mixed bacterial agent includes the following steps:
B1. inoculating streptomyces griseus, bacillus mojavensis, bacillus pumilus and micromonospora in a mass ratio of 1:1:2:1 into a liquid culture medium, adding 0.2wt% of tween into the liquid culture medium, culturing for 48-96 h, and when the number of composite viable bacteria reaches 1 × 10 8 When the concentration is more than CFU/mL, finishing the culture to obtain an activated strain stock solution;
B2. diluting the sesame water by 25 times, adding a liquid culture medium with the volume 5 times that of the sesame water, and culturing for 48-96 h when the number of bacteria is more than 1 multiplied by 10 7 When the concentration is more than CFU/mL, finishing the culture to obtain a magma thallus stock solution;
B3. mixing the magma thallus stock solution and the activated strain stock solution according to the mass ratio of 1:90, carrying out shake culture at 26-28 ℃ for 18h, then carrying out normal-temperature culture for 24-72 h, and when the number of the composite viable bacteria reaches 1 multiplied by 10 8 When the concentration is more than CFU/mL, obtaining a primary culture strain stock solution, continuously mixing the primary culture strain stock solution with an activated strain stock solution for gradual expansion culture, wherein the number of stages is 5, the cultured ephedra thallus stock solution and the activated strain stock solution are sequentially 2:90, 4:90, 8:90, 16:90 and 32:90 in mass ratio, obtaining a deodorant strain stock solution, and drying the deodorant strain stock solution at low temperature to obtain the mixed microbial inoculum.
The liquid medium contained the following components: 35g/L glucose, 2mg/L ammonium chloride, 350mg/L calcium chloride, 150mg/L inositol, 800mg/L monopotassium phosphate, 250 mg/L magnesium sulfate, 18mg/L ferrous sulfate, 8mg/L manganese sulfate, 3mg/L zinc sulfate, 0.3mg/L sodium molybdate and 0.03mg/L copper sulfate, and adjusting the pH to 5.5 by using boric acid and acetic acid.
Example 2
A method for deodorizing hemp water comprises the following steps:
s1, filtering hemp water, and removing hemp residues;
s2, alkalizing the hemp water after removing the hemp residues through an alkaline filler to obtain alkalized hemp water;
and S3, adding the mixed microbial inoculum into the alkalized hemp liquid, and fermenting for 10 days to obtain the deodorized hemp liquid.
The preparation method of the alkaline filler comprises the following steps:
A1. soaking sisal fibers in a saturated calcium hydroxide solution, adding vitamin C, disodium ethylene diamine tetraacetate and hydroxyethyl cellulose, stirring, and freeze-drying to obtain alkalized sisal fibers, wherein the mass of the vitamin C is 0.001% of that of the sisal fibers, and the mass of the disodium ethylene diamine tetraacetate is 0.15% of that of the calcium hydroxide; A2. placing the ceramsite into a colloidal solution of sodium carboxymethylcellulose, wherein the mass fraction of the sodium carboxymethylcellulose is 1.5%, stirring, taking out, placing into a calcium hydroxide saturated solution at normal temperature, slowly heating at a speed of 1 ℃/min, keeping the temperature for 30min when the temperature is raised to 95 ℃, taking out the ceramsite, and drying to obtain the alkalized ceramsite; A3. taking a silicate aqueous solution with the modulus of 2.0, mixing the silicate aqueous solution with water according to the mass ratio of 1:1.2, mixing the sisal fibers, the ceramsite and the silicate aqueous solution according to the weight ratio of 18:45:25, putting the mixture, the alkalized sisal fibers and the alkalized ceramsite into a mold together, mixing the sisal fibers, the ceramsite and the silicate aqueous solution according to the weight ratio of 20:45:22, filling the mixture for 6min by blowing nitrogen, and carrying out freeze drying to obtain the alkaline filler.
In step S3, the method for preparing the mixed bacterial agent includes the following steps:
B1. inoculating streptomyces griseus, bacillus mojavensis, bacillus pumilus and micromonospora in a mass ratio of 1:1:2:1 into a liquid culture medium, adding 0.2wt% of tween into the liquid culture medium, culturing for 48-96 h, and when the number of composite viable bacteria reaches 1 × 10 8 When the concentration is more than CFU/mL, finishing the culture to obtain an activated strain stock solution;
B2. diluting the sesame water by 25 times, adding a liquid culture medium with the volume 5 times that of the sesame water, and culturing for 48-96 h when the number of bacteria is more than 1 multiplied by 10 7 When the concentration is more than CFU/mL, finishing the culture to obtain a magma thallus stock solution;
B3. mixing the magma thallus stock solution and the activated strain stock solution according to the mass ratio of 1:90, carrying out shake culture at 26-28 ℃ for 18h, then carrying out normal-temperature culture for 24-72 h, and when the number of the composite viable bacteria reaches 1 multiplied by 10 8 And when the concentration is more than CFU/mL, obtaining a primary culture strain stock solution, continuously mixing the primary culture strain stock solution with the activated strain stock solution for gradual expansion culture, wherein the number of stages is 5, the cultured ephedra thallus stock solution and the activated strain stock solution are sequentially in a mass ratio of 2:90, 4:90, 8:90, 16:90 and 32:90 to obtain a deodorant strain stock solution, and drying the deodorant strain stock solution at a low temperature to obtain the mixed microbial inoculum.
The liquid medium contained the following components: 35g/L glucose, 2mg/L ammonium chloride, 350mg/L calcium chloride, 150mg/L inositol, 800mg/L monopotassium phosphate, 250 mg/L magnesium sulfate, 18mg/L ferrous sulfate, 8mg/L manganese sulfate, 3mg/L zinc sulfate, 0.3mg/L sodium molybdate and 0.03mg/L copper sulfate, and the pH is adjusted to 5.5 by boric acid and acetic acid.
Example 3
A method for deodorizing hemp water comprises the following steps:
s1, filtering hemp water, and removing hemp residues;
s2, alkalizing the hemp water after removing the hemp residues through an alkaline filler to obtain alkalized hemp water;
and S3, adding the mixed microbial inoculum into the alkalized hemp liquid, and fermenting for 10 days to obtain the deodorized hemp liquid.
The preparation method of the alkaline filler comprises the following steps:
A1. soaking sisal fibers in a saturated calcium hydroxide solution, adding ethylenediamine tetraacetic acid, sodium citrate and polyacrylamide, stirring, and freeze-drying to obtain alkalized sisal fibers, wherein the mass of the ethylenediamine tetraacetic acid is 0.05% of that of the sisal fibers, and the mass of the sodium citrate is 0.2% of that of the calcium hydroxide; A2. placing the ceramsite into a colloidal solution of sodium carboxymethylcellulose, wherein the mass fraction of the sodium carboxymethylcellulose is 1%, stirring, taking out, placing into a calcium hydroxide saturated solution at normal temperature, slowly heating at a speed of 0.5 ℃/min, keeping the temperature for 20min when the temperature is raised to 95 ℃, taking out the ceramsite, and drying to obtain the alkalized ceramsite; A3. taking a silicate aqueous solution with the modulus of 1.8, mixing the silicate aqueous solution with water according to the mass ratio of 1:1, putting the mixture, the alkalized sisal fiber and the alkalized ceramsite into a mold together, mixing the sisal fiber, the ceramsite and the silicate aqueous solution according to the weight ratio of 18:45:25, blowing nitrogen gas for filling for 5min, and carrying out freeze drying to obtain the alkaline filler.
In step S3, the method for preparing the mixed bacterial agent includes the following steps:
B1. streptomyces griseus, Bacillus mojavensis, Bacillus pumilus and Micromonospora according to the mass ratio of 2:2:3:1, inoculating into a liquid culture medium, adding 0.2wt% of Tween into the liquid culture medium,culturing for 48-96 h, when the number of the composite viable bacteria reaches 1 multiplied by 10 8 When the concentration is more than CFU/mL, finishing the culture to obtain an activated strain stock solution;
B2. diluting the sesame water by 30 times, adding a liquid culture medium with the volume of 4 times that of the sesame water, and culturing for 48-96 h when the number of bacteria is more than 1 multiplied by 10 7 When the concentration is more than CFU/mL, finishing the culture to obtain a magma thallus stock solution;
B3. mixing the stock solution of the leprosy thallus with the stock solution of the activated strain according to the mass ratio of 1:95, carrying out shake culture at 26-28 ℃ for 18h, and then carrying out normal-temperature culture for 24-72 h until the number of the composite viable bacteria reaches 1 multiplied by 10 8 And when the ratio of the strains to the strains is more than CFU/mL, obtaining a primary culture strain stock solution, continuously mixing the primary culture strain stock solution with the activated strain stock solution for gradual expansion culture, wherein the number of the stages is 5, the cultured ephedra thallus stock solution and the activated strain stock solution are sequentially in a mass ratio of 2:95, 4:95, 8:95, 16:95 and 32:95, obtaining a deodorant strain stock solution, and drying the deodorant strain stock solution at a low temperature to obtain the mixed microbial inoculum.
The liquid medium contained the following components: 40g/L glucose, 3mg/L ammonium chloride, 400mg/L calcium chloride, 100mg/L inositol, 900mg/L potassium dihydrogen phosphate, 200mg/L magnesium sulfate, 20mg/L ferrous sulfate, 10mg/L manganese sulfate, 5mg/L zinc sulfate, 0.5mg/L sodium molybdate and 0.05mg/L copper sulfate, and adjusting the pH value to 6 by using boric acid and acetic acid.
Comparative example 1
In contrast to example 1, only steps S1 and S2 were performed, and step S3 was not performed.
Comparative example 2
Compared to example 1, steps S1 and S3 were performed, and step S2 was not performed.
Comparative example 3
A method for deodorizing hemp water comprises the following steps:
s1, filtering hemp water, and removing hemp residues;
s2, alkalizing the hemp water after removing the hemp residues through an alkaline filler to obtain alkalized hemp water;
and S3, adding the mixed microbial inoculum into the alkalized hemp liquid, and fermenting for 10 days to obtain the deodorized hemp liquid.
The preparation method of the alkaline filler comprises the following steps:
A1. soaking sisal fibers in a saturated calcium hydroxide solution, adding ethylenediaminetetraacetic acid, disodium ethylenediaminetetraacetate and polyacrylamide, wherein the mass of the ethylenediaminetetraacetic acid is 0.05% of that of the sisal fibers, and the mass of the disodium ethylenediaminetetraacetate is 0.2% of that of the calcium hydroxide, stirring, and freeze-drying to obtain the alkalized sisal fibers; and A2, taking a silicate aqueous solution with the modulus of 1.8, mixing the silicate aqueous solution with water according to the mass ratio of 1:1, putting the mixture, the alkalized sisal fibers and the alkalized ceramsite into a mold together, mixing the sisal fibers and the silicate aqueous solution according to the weight part of 63:25, blowing nitrogen gas for filling for 5min, and freeze-drying to obtain the alkaline filler.
In step S3, the method of preparing the mixed bacterial agent is the same as in example 1.
Comparative example 4
A method for deodorizing hemp water comprises the following steps:
s1, filtering hemp water, and removing hemp residues;
s2, alkalizing the hemp water after removing the hemp residues through an alkaline filler to obtain alkalized hemp water;
and S3, adding the mixed microbial inoculum into the alkalized hemp liquid, and fermenting for 10 days to obtain the deodorized hemp liquid.
The preparation method of the alkaline filler comprises the following steps:
A1. placing the ceramsite into a colloidal solution of sodium carboxymethylcellulose, wherein the mass fraction of the sodium carboxymethylcellulose is 1%, stirring, taking out, placing into a saturated solution of calcium hydroxide at normal temperature, slowly heating to 95 ℃ at a speed of 0.5 ℃/min, keeping the temperature for 20min, taking out the ceramsite, and drying to obtain the alkalized ceramsite; and A2, taking a silicate aqueous solution with the modulus of 1.8, mixing the silicate aqueous solution with water according to the mass ratio of 1:1, putting the mixture, the alkalized ceramsite and the alkalized ceramsite into a mold together, mixing sisal fiber and the silicate aqueous solution according to the weight ratio of 63:25, blowing nitrogen gas for filling for 5min, and freeze-drying to obtain the alkaline filler.
In step S3, the method of preparing the mixed bacterial agent is the same as in example 1.
Comparative example 5
A method for deodorizing hemp water comprises the following steps:
s1, filtering hemp water, and removing hemp residues;
s2, alkalizing the hemp water after removing the hemp residues through an alkaline filler to obtain alkalized hemp water;
and S3, adding the mixed microbial inoculum into the alkalized hemp liquid, and fermenting for 10 days to obtain the deodorized hemp liquid.
The basic filler was prepared in the same manner as in example 1.
In step S3, the method for preparing the mixed bacterial agent includes the following steps:
B1. inoculating the bacillus mojavensis and the bacillus pumilus into a liquid culture medium according to the mass ratio of 1:2, adding 0.2wt% of tween into the liquid culture medium, culturing for 48-96 h, and when the number of the composite viable bacteria reaches 1 multiplied by 10 8 When the concentration is more than CFU/mL, finishing the culture to obtain an activated strain stock solution;
B2. diluting the sesame water by 25 times, adding a liquid culture medium with the volume 5 times that of the sesame water, and culturing for 48-96 h when the number of bacteria is more than 1 multiplied by 10 7 When the concentration is more than CFU/mL, finishing the culture to obtain a magma thallus stock solution;
B3. mixing the magma thallus stock solution and the activated strain stock solution according to the mass ratio of 1:90, carrying out shake culture at 26-28 ℃ for 18h, then carrying out normal-temperature culture for 24-72 h, and when the number of the composite viable bacteria reaches 1 multiplied by 10 8 And when the concentration is more than CFU/mL, obtaining a primary culture strain stock solution, continuously mixing the primary culture strain stock solution with the activated strain stock solution for gradual expansion culture, wherein the number of stages is 5, the cultured ephedra thallus stock solution and the activated strain stock solution are sequentially in a mass ratio of 2:90, 4:90, 8:90, 16:90 and 32:90 to obtain a deodorant strain stock solution, and drying the deodorant strain stock solution at a low temperature to obtain the mixed microbial inoculum.
The liquid medium contained the following components: 35g/L glucose, 2mg/L ammonium chloride, 350mg/L calcium chloride, 150mg/L inositol, 800mg/L monopotassium phosphate, 250 mg/L magnesium sulfate, 18mg/L ferrous sulfate, 8mg/L manganese sulfate, 3mg/L zinc sulfate, 0.3mg/L sodium molybdate and 0.03mg/L copper sulfate, and adjusting the pH to 5.5 by using boric acid and acetic acid.
Odor test examples
The Japanese class 6 odor intensity scale representation of this example characterizes odor as no odor at level 0, barely perceptible mild odor at level 1 (sensory threshold), easily perceptible weak odor at level 2 (recognition threshold), clearly perceptible odor at level 3, strong odor at level 4, and intolerable strong odor at level 5.
The untreated hemp water and the hemp water treated in the examples 1 to 3 and the comparative examples 1 to 5 are naturally placed for 5 days in an environment of 30 ℃, and the odor of the hemp water is characterized by adopting the method.
Table 1 odor rating table
Figure DEST_PATH_IMAGE001
As shown in the table 1, the combination of the alkaline filler prepared by mixing the sisal fibers and the ceramsite and the biological agent can obviously remove the odor of the hemp water.
Diluting the hemp water treated in the embodiment 1-3 by 100 times, using the hemp water as a liquid fertilizer for planting Chinese cabbages, and treating the Chinese cabbages on a Chinese cabbage planting field for 3 times: 1. no fertilization blank treatment (CK group); 2. only applying 50 kg/mu of farmyard manure base fertilizer; 3. the method comprises the steps of taking the hemp water obtained in the embodiments 1-3, applying 50 kg/mu of farmyard manure base fertilizer and 5 kg/mu of hemp liquid fertilizer core covering period, diluting the hemp water treated in the embodiments 1-3 by 100 times when the hemp water is used, uniformly mixing the hemp water and water according to the weight ratio of 1:100, and uniformly spraying the mixture on a Chinese cabbage planting land. The spraying areas of the CK group, the base fertilizer group and the liquid fertilizer for the sesame seeds in the embodiments 1 to 3 are respectively 3 mu, and the yield is increased as shown in a table 2:
TABLE 2
Figure DEST_PATH_IMAGE002
The treated hemp water is used as a liquid fertilizer, so that the yield is remarkably increased, and compared with a base fertilizer group, the yield is increased by 20.37-27.66%.

Claims (7)

1. A method for deodorizing hemp water is characterized by comprising the following steps:
s1, filtering hemp water, and removing hemp residues;
s2, alkalizing the hemp water after removing the hemp residues through an alkaline filler to obtain alkalized hemp water;
s3, adding the alkalized hemp water into a mixed microbial inoculum, and fermenting for 5-10 days to obtain deodorized hemp water;
the preparation method of the alkaline filler comprises the following steps:
A1. soaking sisal fiber in calcium hydroxide solution, adding stabilizer, chelating agent and binder, stirring, and freeze drying; obtaining the alkalized sisal fiber;
A2. placing the ceramsite into a colloidal solution of sodium carboxymethylcellulose, stirring, taking out, placing into a calcium hydroxide saturated solution at normal temperature, slowly heating at the speed of 0.5-1 ℃/min, keeping the temperature for 10-30 min when the temperature is raised to 90-99 ℃, taking out the ceramsite, and drying to obtain the alkalized ceramsite;
A3. taking a silicate aqueous solution with the modulus of 1.5-2.0, mixing the silicate aqueous solution with water according to the mass ratio of 0.5-1.2: 0.5-2, putting the mixture, the alkalized sisal fiber and the alkalized ceramsite into a mold, blowing nitrogen to fill for 5-10 min, and freeze-drying to obtain the alkaline filler.
2. The method of deodorizing hemp water according to claim 1, characterized in that:
in the step A1, the stabilizer is vitamin C or ethylenediaminetetraacetic acid, and the chelating agent is disodium ethylenediaminetetraacetate, sodium citrate, and hydroxyethyl ethylenediaminetriacetic acid trisodium salt; the binder is water-soluble cellulose or polyacrylamide.
3. The method of deodorizing hemp water according to claim 1, characterized in that:
the mass of the stabilizer is 0.001-0.1% of that of the sisal fiber, and the mass of the chelating agent is 0.1-0.8% of that of the calcium hydroxide.
4. The method of deodorizing hemp water according to claim 1, characterized in that:
the sisal fibers, the ceramsite and the silicate water solution are mixed according to the weight part ratio of 16-20: 40-55: 20-30.
5. The method of deodorizing hemp water according to claim 1, characterized in that:
in the step S3, the preparation method of the mixed bacterial agent includes the following steps:
B1. inoculating streptomyces griseus, bacillus mojavensis, bacillus pumilus and micromonospora in a mass ratio of 1-2: 2-3: 1 into a liquid culture medium, adding 0.1-0.5 wt% of tween into the liquid culture medium, culturing for 48-96 h, and when the number of composite viable bacteria reaches 1 x 10 8 When the concentration is more than CFU/mL, finishing the culture to obtain an activated strain stock solution;
B2. diluting the sesame water by 20-30 times, adding a liquid culture medium with the volume of 2-5 times of the sesame water, and culturing for 48-96 hours when the number of bacteria is more than 1 multiplied by 10 7 When the concentration is more than CFU/mL, finishing the culture to obtain a magma thallus stock solution;
B3. mixing the magma thallus stock solution and the activated strain stock solution according to the mass ratio of 1: 50-100, carrying out shake culture at 25-28 ℃ for 10-18 h, then carrying out normal-temperature culture for 24-72 h, and when the number of composite viable bacteria reaches 1 x 10 8 When the concentration is more than CFU/mL, obtaining primary culture strain stock solution, continuously mixing with the activated strain stock solution for gradual expansion culture to obtain deodorant strain stock solution, and drying the deodorant strain stock solution at low temperature to obtain the mixed microbial inoculum.
6. The method of deodorizing hemp water according to claim 5, characterized in that:
the liquid medium contained the following components: 30-40 g/L glucose, 1.5-3 mg/L ammonium chloride, 300-400 mg/L calcium chloride, 100-200 mg/L inositol, 500-900 mg/L monopotassium phosphate, 200-300 mg/L magnesium sulfate, 15-20 mg/L ferrous sulfate, 5-10 mg/L manganese sulfate, 2-5 mg/L zinc sulfate, 0.2-0.5 mg/L sodium molybdate and 0.02-0.05 mg/L copper sulfate, and regulating the pH to 5-6 by using boric acid and acetic acid.
7. The application of the deodorized hemp water obtained by the hemp water deodorization method according to any one of claims 1 to 6 in liquid fertilizer.
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