CN112691710A - Micro-fluidic system - Google Patents
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- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The application discloses a microfluidic system includes: the device comprises a first storage container, a second storage container, a first connecting pipe, a second connecting pipe, an adapter, a third connecting pipe, a microfluidic module, a microscope and an analysis terminal; the first storage container is used for storing a background solution during an experiment; the second storage container is used for storing the experimental solution during the experiment; the first connecting pipe is connected with the first storage container and the adapter; the second connecting pipe is connected with the second storage container and the adapter; the adapter is used for injecting the background solution and/or the experimental solution into the microfluidic module through the third connecting pipe; the microfluidic module is used for providing a flow chamber for observing pollutant deposition in a background solution and/or an experimental solution; the microscope is used for observing pollutant deposition in the microfluidic module; the analysis terminal is used for analyzing the deposition rate of pollutant deposition, and solves the technical problems that the existing microfluidic technology can only qualitatively research the influence of experimental conditions on pollutant deposition, but cannot quantitatively research the pollutant deposition under the experimental conditions.
Description
Technical Field
The application belongs to the technical field of pollutant detection, and particularly relates to a micro-fluidic system.
Background
With the rapid development of economy, the types and amounts of pollutants in the environment are also rapidly increasing. Since the pollutants can directly or indirectly affect the human body, the detection technology of the pollutants is very important.
The micro-fluidic technology has a great deal of application in pollutant detection due to the characteristics of few experimental samples, high efficiency, good separation and enrichment performance and the like. However, the existing microfluidic technology can only qualitatively research the influence of experimental conditions on pollutant deposition, but cannot quantitatively research the pollutant deposition under the experimental conditions.
Disclosure of Invention
In view of this, the present application provides a microfluidic system, which solves the technical problem that the existing microfluidic technology can only qualitatively research the influence of experimental conditions on pollutant deposition, but cannot quantitatively research pollutant deposition under the experimental conditions.
The present application provides a microfluidic system comprising: the device comprises a first storage container, a second storage container, a first connecting pipe, a second connecting pipe, an adapter, a third connecting pipe, a microfluidic module, a microscope and an analysis terminal;
the first storage container is used for storing a background solution during an experiment;
the second storage container is used for storing an experiment solution during an experiment;
one end of the first connecting pipe is connected with the first storage container, and the other end of the first connecting pipe is connected with the adapter;
one end of the second connecting pipe is connected with the second storage container, and the other end of the second connecting pipe is connected with the adaptor;
the adapter is used for injecting the background solution and/or the experimental solution into the microfluidic module through the third connecting pipe;
the microfluidic module is used for providing a flow chamber for observing the deposition of pollutants in the background solution and/or the experimental solution;
the microscope is aligned with the microfluidic module and is used for observing the pollutant deposition in the microfluidic module;
the analysis terminal is connected to the microscope and is configured to analyze a deposition rate of the contaminant deposit.
Alternatively,
the first storage container is a first syringe;
the second storage container is a second syringe.
Alternatively,
further comprising: the system comprises a first unit pump, a second unit pump, a first control terminal and a second control terminal;
the first injector is mounted on the first unit pump, and a unit pump piston of the first unit pump moves axially along the first injector;
the second injector is mounted on the second monomer pump, and a monomer pump piston of the second monomer pump axially moves along the second injector;
the first control terminal is connected with the first unit pump;
and the second control terminal is connected with the second monomer pump.
Alternatively,
the adaptor is a three-way valve.
Alternatively,
the first storage container is a first storage box;
the second storage container is a second storage tank.
Alternatively,
further comprising: a first and a second microfluidic pump;
the first micro-flow pump is connected with the first connecting pipe and used for pumping the background solution from the first storage tank to the first connecting pipe;
and the second micro-flow pump is connected with the second connecting pipe and used for pumping the experimental solution from the second storage tank to the second connecting pipe.
Alternatively,
further comprising: a first flow rate measuring unit and a second flow rate measuring unit;
the first flow rate measuring unit is arranged on the first connecting pipe and used for measuring a first flow rate of the background solution in the first connecting pipe;
the second flow rate measuring unit is arranged on the second connecting pipe and used for measuring a second flow rate of the experimental solution in the second connecting pipe.
Alternatively,
the adaptor is a changeover valve.
Alternatively,
the light source of the microscope includes: a white light source and a fluorescent light source.
Alternatively,
the microscope includes a plurality of fluorescent filters for filtering fluorescence provided by a fluorescent light source.
According to the technical scheme, the method has the following advantages:
the present application provides a microfluidic system comprising: the device comprises a first storage container, a second storage container, a first connecting pipe, a second connecting pipe, an adapter, a third connecting pipe, a microfluidic module, a microscope and an analysis terminal; the first storage container is used for storing a background solution during an experiment; the second storage container is used for storing the experimental solution during the experiment; one end of the first connecting pipe is connected with the first storage container, and the other end of the first connecting pipe is connected with the adapter; one end of the second connecting pipe is connected with the second storage container, and the other end of the second connecting pipe is connected with the adapter; the adapter is used for injecting the background solution and/or the experimental solution into the microfluidic module through the third connecting pipe; a microfluidic module for providing a flow cell for observing deposition of contaminants in a background solution and/or an experimental solution; the microscope is aligned with the microfluidic module and is used for observing pollutant deposition in the microfluidic module; the analysis terminal is connected to the microscope and is used to analyze the deposition rate of contaminant deposits.
Through first storage vessel, first connecting pipe, the adaptor, the third connecting pipe injects background solution into micro-fluidic module in this application, through the second storage vessel, the second connecting pipe, the adaptor, the third connecting pipe injects experimental solution into micro-fluidic module, the micro-fluidic module is aimed at to the microscope, can observe the pollutant deposit in micro-fluidic module background solution and the experimental solution through the microscope like this, and analysis terminal connects the microscope, the sedimentary deposition rate of pollutant deposit that observes to the microscope analyzes, it can only qualitatively research the influence of experimental condition to the pollutant deposit to have solved current micro-fluidic technique, can not quantitatively research the sedimentary technical problem of pollutant under the experimental condition.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise.
Fig. 1 is a schematic structural diagram of a microfluidic system according to an embodiment of the present disclosure;
fig. 2 is another schematic structural diagram of a microfluidic system according to an embodiment of the present disclosure;
FIG. 3 is a schematic structural diagram of a microfluidic module according to an embodiment of the present disclosure;
FIG. 4 is a schematic cross-sectional view taken at A-A of FIG. 3;
FIG. 5 is a graph of the number of microspheres deposited versus time in comparative example one;
FIG. 6 is a graph comparing the deposition of microspheres at different salinity in comparative example two;
FIG. 7 is a graph comparing the deposition of microspheres at different salinity in comparative example III;
wherein the reference numbers are as follows:
1. analyzing the terminal; 2. a vacuum pump; 3. a microscope; 31. an object stage; 32. an image pickup unit; 4. a microfluidic module; 41. a liquid inlet pipeline; 42. a liquid outlet pipeline; 43. a vacuum line; 44. a three-way valve; 45. a microfluidic module upper element; 46. a vacuum tank; 47. sealing gaskets; 48. a quartz plate; 49. a tapered flow passage; 5. a changeover valve; 61. a first microfluidic pump; 62. a first flow rate measuring unit; 63. a first storage tank; 71. a second microfluidic pump; 72. a second flow rate measuring unit; 73. a second storage tank; 8. a pneumatic pump; 101. a first syringe; 102. a first syringe pump; 103. a first control terminal; 111. a second syringe; 112. a second syringe pump; 113. and a second control terminal.
Detailed Description
The embodiment of the application provides a microfluidic system, and solves the technical problems that the existing microfluidic technology can only qualitatively research the influence of experimental conditions on pollutant deposition, but cannot quantitatively research the pollutant deposition under the experimental conditions.
The technical solutions of the embodiments of the present application will be described clearly and completely with reference to the accompanying drawings, and it is obvious that the described embodiments are some, but not all, of the embodiments of the present application. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application without any creative effort belong to the protection scope of the embodiments in the present application.
In the description of the embodiments of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing the embodiments of the present application and simplifying the description, but do not indicate or imply that the referred devices or elements must have specific orientations, be configured in specific orientations, and operate, and thus, should not be construed as limiting the embodiments of the present application. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the embodiments of the present application, it should be noted that the terms "mounted," "connected," and "connected" are used broadly and are defined as, for example, a fixed connection, an exchangeable connection, an integrated connection, a mechanical connection, an electrical connection, a direct connection, an indirect connection through an intermediate medium, and a communication between two elements, unless otherwise explicitly stated or limited. Specific meanings of the above terms in the embodiments of the present application can be understood in specific cases by those of ordinary skill in the art.
The present application provides a first embodiment of a microfluidic system, and in particular, please refer to fig. 1.
The microfluidic system in this embodiment includes: the device comprises a first storage container, a second storage container, a first connecting pipe, a second connecting pipe, an adapter, a third connecting pipe, a microfluidic module 4, a microscope 3 and an analysis terminal 1; the first storage container is used for storing a background solution during an experiment; the second storage container is used for storing the experimental solution during the experiment; one end of the first connecting pipe is connected with the first storage container, and the other end of the first connecting pipe is connected with the adapter; one end of the second connecting pipe is connected with the second storage container, and the other end of the second connecting pipe is connected with the adapter; the adaptor is used for injecting the background solution and/or the experimental solution into the microfluidic module 4 through the third connecting pipe; a microfluidic module 4 for providing a flow chamber for observing the deposition of contaminants in the background solution and/or the experimental solution; the microscope 3 is aligned with the microfluidic module 4, and the microscope 3 is used for observing pollutant deposition in the microfluidic module 4; the analysis terminal 1 is connected to a microscope 3, and the analysis terminal 1 is used to analyze the deposition rate of contaminant deposition.
It should be noted that the adaptor is used for injecting the background solution and/or the experimental solution into the microfluidic module 4 through the third connection tube. Specifically, when the pollutant deposition of the background solution needs to be observed, the background solution is injected into the microfluidic module 4; when the pollutant deposition of the experimental solution needs to be observed, the experimental solution is injected into the microfluidic module 4; typically, the background solution and the experimental solution are reacted to observe the deposition of the contaminant, and both the background solution and the experimental solution are injected into the microfluidic module 4.
It will be appreciated that the analysis terminal 1 may be a computer or other device having data processing and data analysis functions. The analysis terminal 1 takes an image of the microscope 3 by means of the camera unit 32, and the analysis terminal 1 analyzes the taken image by computer vision techniques to obtain the deposition rate of the contaminant deposit.
In this embodiment, a background solution is injected into the microfluidic module 4 through the first storage container, the first connection pipe, the adaptor, and the third connection pipe, an experimental solution is injected into the microfluidic module 4 through the second storage container, the second connection pipe, the adaptor, and the third connection pipe, the microscope 3 is aligned with the microfluidic module 4, so that the deposition of contaminants in the background solution and the experimental solution of the microfluidic module 4 can be observed through the microscope 3, the analysis terminal 1 is connected with the microscope 3, and the deposition rate of the contaminant deposition observed by the microscope 3 is analyzed.
The above is a first embodiment of a microfluidic system provided in the present application, and the following is a second embodiment of a microfluidic system provided in the present application.
The microfluidic system in this embodiment includes: the device comprises a first storage container, a second storage container, a first connecting pipe, a second connecting pipe, an adapter, a third connecting pipe, a microfluidic module 4, a microscope 3 and an analysis terminal 1; the first storage container is used for storing a background solution during an experiment; the second storage container is used for storing the experimental solution during the experiment; one end of the first connecting pipe is connected with the first storage container, and the other end of the first connecting pipe is connected with the adapter; one end of the second connecting pipe is connected with the second storage container, and the other end of the second connecting pipe is connected with the adapter; the adaptor is used for injecting the background solution and/or the experimental solution into the microfluidic module 4 through the third connecting pipe; a microfluidic module 4 for providing a flow chamber for observing the deposition of contaminants in the background solution and/or the experimental solution; the microscope 3 is aligned with the microfluidic module 4, and the microscope 3 is used for observing pollutant deposition in the microfluidic module 4; the analysis terminal 1 is connected to a microscope 3, and the analysis terminal 1 is used to analyze the deposition rate of contaminant deposition.
It is to be understood that, as shown in fig. 1, the first storage container in the present embodiment is a first storage box 63; the second storage container is a second storage tank 73.
Further, in order to smoothly inject the background solution in the first storage tank 63 and the experimental solution in the second storage tank 73 into the microfluidic module 4, the microfluidic system in this embodiment further includes: a first and a second micro-flow pump 61, 71; the first micro-flow pump 61 is connected with the first connecting pipe and is used for pumping the background solution from the first storage tank 63 to the first connecting pipe; the second micro-flow pump 71 is connected to the second connection pipe, and is used for pumping the test solution from the second storage tank 73 to the second connection pipe. It should be noted that, because the first and second micro-flow pumps 61 and 71 can achieve a relatively accurate adjustment accuracy, the background solution and the experimental solution can be adjusted more accurately by the first and second micro-flow pumps 61 and 71, which is beneficial for the analysis of pollutant deposition by the personnel. Specifically, in order to control the speed of the microfluidic pump when pumping the solution, the microfluidic system in this embodiment further includes: a first flow rate measuring unit 62 and a second flow rate measuring unit 72; the first flow rate measuring unit 62 is arranged on the first connecting pipe and used for measuring a first flow rate of the background solution in the first connecting pipe; the second flow rate measuring unit 72 is disposed on the second connecting pipe, and is used for measuring a second flow rate of the test solution in the second connecting pipe.
The first flow rate measuring unit 62 and the second flow rate measuring unit 72 are used for accurately measuring the flow rate of the background solution or the experimental solution in the pipeline, so that the pressure in the micro-flow pump can be adjusted conveniently to obtain the required flow rate.
It will be appreciated that the adaptor in this embodiment is a changeover valve 5.
Specifically, the first storage box 63 is made of plastic and is sealed by a cover connected with the first micro-flow pump 61, so that the air tightness in the whole container is ensured, and the first micro-flow pump 61 can work normally. Further, for the extraction of the convenient experimental solution, the bottom of first bin 63 sets up to leak hopper-shaped for experimental solution assembles to the funnel lower part, prevents the condition that the absorption air appears in the experiment.
Similarly, the second storage tank 73 is made of plastic and is sealed by a cover connected with the second micro-flow pump 71, so that the air tightness in the whole container is ensured, and the second micro-flow pump 71 can work normally. Further, in order to facilitate the extraction of the background solution, the bottom of the second storage tank 73 is set to be funnel-shaped, so that the background solution is gathered towards the lower part of the funnel, and the situation of sucking air in the experiment is prevented.
The conversion valve 5 is used for controlling the type of the solution entering the microfluidic module 4, is provided with a button for switching the inflow solution, and is provided with an indicator light for indicating the type of the solution flowing into the microfluidic module 4.
It can be understood that, in order to provide the pressure for controlling the liquid flow by the micro-flow pump, the micro-flow pump is connected with the pneumatic pump 8, a vacuum tube connected with the micro-flow pump is arranged outside the pneumatic pump, and a pressure reducing valve is arranged in the middle of the vacuum tube. The pneumatic pump 8 is used for providing pressure required for transporting the solution for the first micro-flow pump 61 and the second micro-flow pump 71. The pressure reducing valve is connected with an outlet of the pneumatic pump 8, and is connected with the first micro-flow pump 61 and the second micro-flow pump 71, so that the air pressure entering the first micro-flow pump 61 and the second micro-flow pump 71 is controlled, or the redundant pressure is released.
It is to be understood that, as shown in fig. 2, the first storage container in this embodiment is a first syringe 101; the second storage container is a second syringe 111.
Specifically, in order to control the first syringe 101 and the second syringe 111 to automatically inject the background solution and the experimental solution into the microfluidic module 4, the microfluidic system in this embodiment further includes: a first unit pump, a second unit pump, a first control terminal 103 and a second control terminal 113; a first injector 101 is installed on the first monomer pump, and a monomer pump piston of the first monomer pump axially moves along the first injector 101; a second injector 111 is mounted on the second single pump, and a single pump piston of the second single pump axially moves along the second injector 111; the first control terminal 103 is connected with the first monomer pump; the second control terminal 113 is connected to the second unit pump. It will be appreciated that the adaptor in this case is a three-way valve 44.
The background solution, the test solution, and the like may be extracted by a micro-flow pump as shown in fig. 1, or may be extracted by a syringe pump as shown in fig. 2. Specifically, those skilled in the art can set the configuration by themselves, only one of the structures may be selected, or both of the structures may be designed, and details thereof are not described herein.
As shown in fig. 1 and 2, the light source of the microscope 3 in the present embodiment includes: a white light source and a fluorescent light source. The white light source and the fluorescent light source are respectively used for providing white light and fluorescence for the microscope 3, so that direct observation of pollutants in the flow chamber of the microfluidic module 4 is facilitated.
Further, the microscope 3 further includes: a plurality of fluorescent filters; the fluorescence filter is used for filtering fluorescence provided by the fluorescence light source. The fluorescence filter filters the fluorescence provided by the fluorescence light source, only allows the fluorescence with a specific wavelength to pass through, and is beneficial to observing the pollutant deposition condition in the micro-flow control flow chamber.
In this embodiment, the microscope 3 includes the stage 31 capable of accurately controlling the spatial position, the stage 31 can move along the x-axis and y-axis directions of the plane, and the height of the stage in the z-axis direction is fixed, so that the microscope 3 can be prevented from being defocused, and the pollutant deposition at different flow channel positions in the microfluidic module 4 can be observed and analyzed conveniently.
Further, the camera unit 32 in this embodiment is connected to the microscope 3 and the analysis terminal 1, and the camera unit 32 is used for shooting deposition conditions of pollutants at different positions in the flow channel of the microfluidic module 4 at different times, which is helpful for further recording and analyzing the deposition phenomenon of pollutants in the environment simulated by the microfluidic module 4.
Further, the microscope 3 includes a differential interference component, and the differential interference component is used for converting white light provided by white light into polarized light, which is beneficial to observing three-dimensional stereo projection images of microorganisms such as bacteria.
Further, the microfluidic system in this embodiment further includes a rocker device, the rocker device is connected to the microscope 3, the object stage 31, and the analysis terminal 1, and the rocker device is configured to display positions of the object stage 31 along an x-axis, a y-axis, and a z-axis in a space, and can control movement of the object stage 31 in the x-axis and y-axis directions and a position of a lens of the microscope 3 in the z-axis direction, so as to observe and analyze deposition conditions of contaminants at different positions of the flow channel, and facilitate analysis of influences of different factors on deposition phenomena of the contaminants.
Further, the analysis terminal 1 in this embodiment is connected to the microfluidic pumps (the first and second microfluidic pumps 61 and 71), the switching valve 5, and the microscope 3 imaging unit 32, and the analysis terminal 1 integrates the microfluidic control software, the microscope 3, the stage 31 control software, and the image processing software. The analysis terminal 1 can control the flow rate of the micro-fluidic pump in real time, so that a stable flow rate is kept in the micro-fluidic module 4, and the influence of fluid flow rate disturbance on the deposition behavior of pollutants in the fluid is eliminated.
Further, the microscope control software integrated in the analysis terminal 1 can take pictures and record videos in the deposition process of the pollutants in the microfluidic module 4, and the image processing software integrated in the analysis terminal 1 can analyze the deposition proportion of the pollutants in the shot pictures or images, so that the analysis of the deposition condition of the pollutants in the microfluidic module 4 is realized.
Further, as shown in fig. 3 and 4, the microfluidic module 4 is formed by combining an upper element 45 of the microfluidic module, a sealing gasket 47 and a quartz plate 48, the upper element 45 of the microfluidic module is provided with a vacuum pipeline 43 and a vacuum groove 46, the sealing gasket 47 is provided with small holes, the vacuum pipeline 43 is connected with the vacuum groove 46, the upper element 45 of the microfluidic module, the sealing gasket 47 and the quartz plate 48 are combined and fixed by applying vacuum to the sealing gasket 47 and the quartz plate 48 which are in contact with the vacuum groove 46, the microfluidic module 4 is provided with a liquid inlet pipeline 41, a liquid outlet pipeline 42 and a rectangular flow chamber, the liquid inlet pipeline 41 and the liquid outlet pipeline 42 are arranged at two sides above the microfluidic module 4, the flowing direction of the liquid in the liquid inlet pipeline 41 and the liquid outlet pipeline 42 is consistent with the flowing direction of the liquid in the flow chamber, the liquid inlet end and the liquid outlet end of the flow chamber of, the tapered flow channel 49 is used for uniformly distributing solution entering and flowing out of the flow chamber to ensure the stability of a flow field in the flow chamber, the narrow end of the tapered flow channel 49 is connected with the liquid inlet pipeline and the liquid outlet pipeline 42, the wide end is connected with the flow chamber, the microfluidic module 4 is provided with a sealing ring and a substrate, the substrate is used for providing different flow chamber simulation environments, the sealing ring is used for providing a closed environment for the flow chamber to prevent air leakage from interfering with the flow chamber, the microfluidic module 4 is provided with two vacuum tubes for ensuring the flow chamber to be closed, the vacuum tubes are arranged at the front part and the rear part of the upper part of the microfluidic module 4 and are connected with the vacuum pump 2, and the vacuum tubes are used.
By adopting the structure of the microfluidic module 4, the stable and uniform flow field in the flow chamber of the microfluidic module 4 is ensured, the disturbance caused by external factors is reduced, and the accuracy and the reliability of the analysis result of the pollutant deposition condition are improved.
In this embodiment, a background solution is injected into the microfluidic module 4 through the first storage container, the first connection pipe, the adaptor, and the third connection pipe, an experimental solution is injected into the microfluidic module 4 through the second storage container, the second connection pipe, the adaptor, and the third connection pipe, the microscope 3 is aligned with the microfluidic module 4, so that the deposition of contaminants in the background solution and the experimental solution of the microfluidic module 4 can be observed through the microscope 3, the analysis terminal 1 is connected with the microscope 3, and the deposition rate of the contaminant deposition observed by the microscope 3 is analyzed.
The analysis effect of the microfluidic system in this example is illustrated below by a specific application comparative example:
comparative example one:
preparing background solution with salinity of 100MMKCl and concentration of 100 MKCl of 5 × 105(one/mL) experimental solutions of fluorescent plastic microspheres (hereinafter referred to as microspheres) are respectively added into a first storage container and a second storage container, and a pneumatic pump 8, a pressure reducing valve, a first micro-flow pump 61, a second micro-flow pump 71 and a switching valve 5 are opened; the background solution in the second storage container sequentially passes through the second flow rate measuring unit 72 and the change-over valve 5, enters from the liquid inlet pipeline 41 at the upper part of the microfluidic module 4, is dispersed through the tapered flow channel 49 and then enters the microfluidic module flow chamber, and flows out from the liquid outlet pipeline 42 through the tapered flow channel after the flow chamber is filled with the background solution; then, the switching valve 5 was switched so that the first storage container and the first flow rate measuring unit 62 were connected to the switching valve 5, and the test solution in the first storage container was supplied at a stable flow rate of 62.5. mu.L/minThe liquid enters the microfluidic module 4 from the liquid inlet pipeline 41 through the first flow velocity measuring unit 62 and the switching valve 5 in sequence, and enters the flow chamber after being dispersed through the conical flow channel 49; and finally, starting the inverted fluorescence microscope 3, the microscope camera 32 and the fluorescence power supply, and adopting a 20-time ocular lens.
Taking fluorescence as a microscope light source on the bottom surface of a flow chamber of a microscope focusing microfluidic control module, setting the exposure time to be 800ms, and selecting five points at different positions with the distance x of 1.5cm away from an inflow position; five points at different positions at a distance x of 2.0cm away from the inflow position; five points at different positions at a distance x of 2.5cm away from the inflow position; and taking 20 points of five points at different positions at a distance x of 3.0cm away from the inflow position as the points of microscope shooting, setting that every 1min of each point is shot for 14min, and starting shooting.
And (3) processing of shot pictures:
after the photo is shot, the static microspheres and the moving microspheres are distinguished by using image recognition software through different fluorescence intensities caused by long exposure time, the static microspheres are counted, deposition rates of the microspheres in the same time under different conditions are compared, deposition behaviors of the microspheres under different conditions can be directly researched, and the influence of other forces such as other hydrodynamic force and rotating force on the deposition of the microspheres is favorably eliminated.
And after counting is finished, recording counting results at different time points for each shooting position, wherein the counting results correspond to the experiment starting time when the picture is shot one by one. Table 1 shows the deposition of 4.5 μm microspheres in the microfluidic module at a distance of 1.5cm from the inflow in the microfluidic module, and the counting results are as follows:
TABLE 1
Table 2 shows the deposition of 4.5 μm microspheres in the microfluidic module at a distance of 2.0cm from the inlet flow, and the counting results are as follows:
TABLE 2
Table 3 shows the deposition of 4.5 μm microspheres in the microfluidic module at a distance of 2.5cm from the inlet flow, and the counting results are as follows:
TABLE 3
Table 4 shows the deposition of 4.5 μm microspheres in the microfluidic module at a distance of 3.0cm from the inflow, and the counting results are as follows:
TABLE 4
And (3) making a scatter diagram with the time point as the abscissa and the deposited colloidal pollutant quantity as the ordinate, fitting the scatter diagram formed by 15 points to obtain the slope of the deposited colloidal pollutant quantity to time after fitting, and taking the obtained slope as the deposition rate of the colloidal pollutant on the shooting position.
The graph of the deposition amount of 4.5 μm microspheres in the microfluidic module at a point 3.0cm from the inflow versus time in the microfluidic module is shown in fig. 5.
For four different distances from the inflow, the deposition rates of five points at different positions on the same inflow distance are averaged to obtain the deposition rate of the colloidal pollutants at the inflow distance.
Comparative example two:
in this comparative example, the test solution had a concentration of 1.88X 106The salinity (per mL) is respectively 1 MKCl and 100 MKCl, and the background solution is 1 MKCl and 100 MKCl salt solution. The microscope objective adopts a 10-fold objective.
The experimental method and the counting means are the same as the comparative example one.
Table 5 shows the deposition of 4.5 μm microspheres in the microfluidic module in the flow chamber, and the test results are as follows:
TABLE 5
A comparison of microsphere deposition at different salinity levels within a microfluidic module is shown in FIG. 6.
And (4) conclusion: the microsphere deposition rate under high salinity measured by the method is far higher than that under low salinity, and is similar to the result of a macro-column experiment.
Comparative example three:
a structure of a micro-fluidic device is shown in fig. 2, a microscope in the comparative example adopts a 20-time objective lens, a polarized light component is switched into the microscope under a white light source, the bottom surface of a flow chamber of a micro-fluidic module is focused, and after exposure time is set to be 800ms, five points at different positions, at which the distance x from an inflow position is 1.5cm, are selected; five points at different positions at a distance x of 2.0cm away from the inflow position; five points at different positions at a distance x of 2.5cm away from the inflow position; and taking 20 points of five points at different positions at a distance x of 3.0cm away from the inflow position as the points of microscope shooting, setting each point to take a picture every 1min, and starting shooting.
And (3) processing of shot pictures: after the photo is shot, static microspheres and moving microspheres are distinguished by different RGB colors caused by long exposure time through image recognition software, the static microspheres are counted, and the deposition behaviors of the microspheres under different conditions are researched.
After counting is finished, a scatter diagram with the time point as the abscissa and the deposited microsphere number as the ordinate is made for each shooting position, the slope of the deposited microsphere number to the time is used as the deposition rate of the colloidal pollutants, and the average value of the deposition rates of five points in the same inflow distance is used as the deposition rate of the microspheres in the inflow distance.
Table 6 shows the deposition of 1.0 μm microspheres in the microfluidic module in the flow chamber, and the test results are as follows:
TABLE 6
A comparison of microsphere deposition at different salinity levels within a microfluidic module is shown in FIG. 7.
And (4) conclusion: the microsphere deposition rate under high salinity measured by the method is far higher than that under low salinity, and is similar to the result of a macro-column experiment.
Compared with the microfluidic device in the first comparative example, the microfluidic device in the second comparative example has the advantages that pipelines are on the same horizontal plane and are short, the influence of gravity on pollutant deposition behaviors is reduced, and the accuracy and the stability of flow rate are influenced by a microfluidic injection pump, an injector and the like in many aspects.
The above embodiments are only used for illustrating the technical solutions of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions in the embodiments of the present application.
Claims (10)
1. A microfluidic system, comprising: the device comprises a first storage container, a second storage container, a first connecting pipe, a second connecting pipe, an adapter, a third connecting pipe, a microfluidic module, a microscope and an analysis terminal;
the first storage container is used for storing a background solution during an experiment;
the second storage container is used for storing an experiment solution during an experiment;
one end of the first connecting pipe is connected with the first storage container, and the other end of the first connecting pipe is connected with the adapter;
one end of the second connecting pipe is connected with the second storage container, and the other end of the second connecting pipe is connected with the adaptor;
the adapter is used for injecting the background solution and/or the experimental solution into the microfluidic module through the third connecting pipe;
the microfluidic module is used for providing a flow chamber for observing the deposition of pollutants in the background solution and/or the experimental solution;
the microscope is aligned with the microfluidic module and is used for observing the pollutant deposition in the microfluidic module;
the analysis terminal is connected to the microscope and is configured to analyze a deposition rate of the contaminant deposit.
2. The microfluidic system of claim 1, wherein the first storage container is a first syringe;
the second storage container is a second syringe.
3. The microfluidic system of claim 2, further comprising: the system comprises a first unit pump, a second unit pump, a first control terminal and a second control terminal;
the first injector is mounted on the first unit pump, and a unit pump piston of the first unit pump moves axially along the first injector;
the second injector is mounted on the second monomer pump, and a monomer pump piston of the second monomer pump axially moves along the second injector;
the first control terminal is connected with the first unit pump;
and the second control terminal is connected with the second monomer pump.
4. The microfluidic system of claim 3, wherein the adapter is a three-way valve.
5. The microfluidic system of claim 1, wherein the first storage container is a first storage tank;
the second storage container is a second storage tank.
6. The microfluidic system of claim 5, further comprising: a first and a second microfluidic pump;
the first micro-flow pump is connected with the first connecting pipe and used for pumping the background solution from the first storage tank to the first connecting pipe;
and the second micro-flow pump is connected with the second connecting pipe and used for pumping the experimental solution from the second storage tank to the second connecting pipe.
7. The microfluidic system of claim 6, further comprising: a first flow rate measuring unit and a second flow rate measuring unit;
the first flow rate measuring unit is arranged on the first connecting pipe and used for measuring a first flow rate of the background solution in the first connecting pipe;
the second flow rate measuring unit is arranged on the second connecting pipe and used for measuring a second flow rate of the experimental solution in the second connecting pipe.
8. The microfluidic system of claim 7, wherein the adapter is a switching valve.
9. The microfluidic system of claim 1, wherein the light source of the microscope comprises: a white light source and a fluorescent light source.
10. The microfluidic system of claim 9, wherein the microscope further comprises: a plurality of fluorescent filters;
the fluorescence filter is used for filtering fluorescence provided by the fluorescence light source.
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