CN112690333A - Normal-temperature recovery lactic acid milk and preparation method thereof - Google Patents

Normal-temperature recovery lactic acid milk and preparation method thereof Download PDF

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CN112690333A
CN112690333A CN202011550417.7A CN202011550417A CN112690333A CN 112690333 A CN112690333 A CN 112690333A CN 202011550417 A CN202011550417 A CN 202011550417A CN 112690333 A CN112690333 A CN 112690333A
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milk
temperature
lactic acid
reconstituted
normal
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苗君莅
李伟
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Bright Dairy and Food Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A23C9/1238Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt using specific L. bulgaricus or S. thermophilus microorganisms; using entrapped or encapsulated yoghurt bacteria; Physical or chemical treatment of L. bulgaricus or S. thermophilus cultures; Fermentation only with L. bulgaricus or only with S. thermophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/1307Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/123Bulgaricus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/249Thermophilus

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
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Abstract

The invention discloses normal temperature recovery lactic acid milk and a preparation method thereof, wherein the preparation raw materials comprise: 6.0-8.5% of cane sugar, 0-0.4% of lactoprotein powder, 1.6-2.0% of compound stabilizer and 0.002-0.01% of leavening agent, and the balance is the restored milk to be 100%; heating the reconstituted milk to 45-55 ℃, adding other ingredients except the leavening agent, and mixing for 15-30 min; homogenizing the obtained materials, sterilizing, and cooling to obtain fermentation substrate; adding a leaven into the fermentation substrate for fermentation, demulsifying after fermenting for 4.5-6.5 h, pasteurizing for the second time, cooling, and aseptically filling. The invention overcomes the problems of taste, stability and the like caused by producing the normal-temperature yoghourt by the reconstituted milk, successfully solves the problem of shortage of the raw milk of the normal-temperature yoghourt with the largest raw milk demand, helps the dairy enterprises to produce the high-quality normal-temperature yoghourt in the 'unsmooth milk' season, and ensures the market supply.

Description

Normal-temperature recovery lactic acid milk and preparation method thereof
Technical Field
The invention relates to the technical field of dairy products, in particular to normal-temperature recovered lactic acid milk and a preparation method thereof.
Background
The yoghourt is a food with good health care function, and has the health care functions of regulating gastrointestinal flora, improving the immune system of the organism, inhibiting tumor, reducing cholesterol level, inhibiting vivotoxin and delaying the aging of the organism. However, because of the wide range of the country, the sale mode and the sale environment of the food are also different, and the problem that the low-temperature yoghourt needs to be kept in cold chain transportation in the whole process is solved, the market of the normal-temperature yoghourt is rapidly developed in recent years, and the annual sale amount of the normal-temperature yoghourt in China reaches hundreds of billions at present. With the increasing favor of people on dairy products, many dairy enterprises can adopt the reconstituted milk to produce the yoghourt particularly in the season of milk source shortage, but the normal-temperature yoghourt produced by the reconstituted milk has rough mouthfeel and unstable system and cannot be stored for a long time at normal temperature due to the special process, storage condition, quality guarantee period and the like of the normal-temperature yoghourt. Therefore, although the usage amount of the raw milk of the normal-temperature yoghourt is huge, the reconstituted milk cannot be used for relieving the problem of lack of milk in the barren period of milk. The invention aims to produce the normal-temperature yoghourt with the largest demand on the raw milk by adopting the reconstituted milk, thereby fundamentally relieving the problem of the ubiquitous shortage of milk sources at present.
Disclosure of Invention
The invention aims to provide normal-temperature recovery lactic acid milk and a preparation method thereof, which overcome the problems of sticky taste, powdery feeling, or excessive low viscosity, water separation, serious layering and precipitation and long fermentation time in normal-temperature storage caused by the production of the normal-temperature yogurt by the recovery milk, and produce the normal-temperature yogurt prepared by the recovery milk.
The invention is realized by the following technical scheme in order to achieve the purpose:
on one hand, the invention provides a preparation method of normal temperature recovery lactic acid milk, which comprises the following raw materials: 6.0-8.5% of cane sugar, 0-0.4% of lactoprotein powder, 1.6-2.0% of compound stabilizer and 0.002-0.01% of leavening agent, and the balance being the restored milk and making up to 100%, wherein the percentages are mass percentages of the total mass of the raw materials; the reconstituted milk is prepared by heating water to 40-50 ℃, adding whole milk powder, stirring and dissolving for 20-30 min, cooling to 4-6 ℃, standing and hydrating for 8-12 hours;
the preparation method of the normal-temperature recovery lactic acid milk comprises the following steps:
(1) heating the reconstituted milk to 45-55 ℃, adding other ingredients except the leavening agent, and mixing for 15-30 min;
(2) homogenizing, sterilizing and cooling the material obtained in the step (1) to obtain a fermentation substrate;
(3) and (3) adding a leaven into the fermentation substrate obtained in the step (2) for fermentation, demulsifying after fermenting for 4.5-6.5 h, performing secondary pasteurization, cooling, performing aseptic filling, and standing at normal temperature.
Further, the reconstituted milk is prepared from 12.4% of whole milk powder and 87.6% of water, and the percentages are percentages of the components in the reconstituted milk raw materials. The used whole milk powder is a conventional raw material and meets the requirement of GB-19644 national food safety standard-milk powder.
Further, the milk protein powder is conventional milk protein powder in the field, preferably whole milk protein powder or whey protein powder with 70-80% of milk protein content, and the percentage is the mass percentage of the milk protein powder.
Further, the compound stabilizer is a colloid stabilizer and modified starch which are compounded according to the proportion of 1: 2-3, and the colloid stabilizer is one or a combination of more of agar, pectin and gellan gum. Modified starch refers to modified starch conventionally used in yogurt products.
Further, the strain of the leaven refers to the leaven 380 which is screened and is suitable for fermenting and restoring the milk base material, and the strain comprises Streptococcus thermophilus (Streptococcus thermophilus) and Lactobacillus bulgaricus (Lactobacillus bulgaricus). The leaven is quick in acid production of fermented and reconstituted milk, and the fermentation time can meet the fermentation time required by large-scale production for 4.5-6.5 h.
Further, the temperature for homogenizing in step (2) is a temperature conventional in the art, and preferably 60-65 ℃. The homogenizing pressure is a conventional pressure in the art, preferably 18 to 25MPa, and more preferably 18 to 20 MPa. The sterilization is the conventional sterilization in the field, preferably the sterilization temperature is 90-135 ℃, and the sterilization time is 5-600 s; the sterilization is preferably pasteurization or UHT, the sterilization temperature is 90-97 ℃, and the sterilization time is 5-10 min.
Further, in the step (3), the fermentation substrate obtained in the step (2) is cooled to 39-43 ℃, and a leavening agent is added for heat preservation and fermentation. Wherein the cooling temperature is more preferably 40-42 ℃. The addition amount of the leavening agent is the conventional addition amount in the field, and the preferable addition amount is 0.002-0.006% (w/w).
Further, the end point of the fermentation in the step (3) is that the titration acidity reaches 70-80 degrees T, preferably 70-75 degrees T.
Further, the secondary pasteurization is carried out at the temperature of 70-80 ℃ for 10-30 s. Sterilizing and cooling to 20-35 ℃.
On the other hand, the invention also provides the normal-temperature recovery lactic acid milk prepared by the preparation method of the normal-temperature recovery lactic acid milk.
On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.
The reagents and starting materials used in the present invention are commercially available.
The positive progress effects of the invention are as follows: the invention provides normal-temperature yoghourt prepared by using reconstituted milk and a preparation method thereof, overcomes the problems of taste, stability and the like caused by producing the normal-temperature yoghourt by using the reconstituted milk, successfully solves the problem of shortage of raw milk of the normal-temperature yoghourt with the largest raw milk demand, helps a dairy enterprise to produce high-quality normal-temperature yoghourt in the 'barren milk' season, and ensures market supply.
Detailed Description
The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention. The experimental methods without specifying specific conditions in the following examples were selected according to the conventional methods and conditions, or according to the commercial instructions.
In the following examples, the sources of the raw materials used were:
whole milk powder: hengyuans commercial (Shanghai) Co., Ltd;
milk protein powder: davilin International trade, Inc. The milk protein powder comprises whole milk protein powder with milk protein content of more than or equal to 70 percent or whey protein powder with milk protein content of more than or equal to 80 percent;
compounding a stabilizer and a leavening agent: danisc (china) investment limited;
fermenting agent 380: kohansen (Beijing) trade company, Inc.
Example 1
A preparation method of normal temperature recovery lactic acid milk comprises the following raw material formula:
Figure BDA0002856974420000041
the preparation method comprises the following steps:
(1) preparing reconstituted milk: heating water to 50 deg.C, adding whole milk powder, stirring for dissolving for 20min, cooling to 6 deg.C, standing for hydrating for 8 hr to obtain reconstituted milk;
(2) heating the reconstituted milk obtained in the step 1 to 45 ℃, adding other ingredients except the leavening agent, and mixing for 30 min;
(3) heating the material obtained in the step (2) to 60 ℃, homogenizing at 60 ℃ and 25Mpa, and sterilizing at 90 ℃ for 600s to obtain a fermentation substrate;
(4) cooling the fermentation substrate obtained in the step (3) to 43 ℃, adding a leavening agent, fermenting for 6.5h under heat preservation until the final acidity is 70 DEG T, killing the bacteria at 70 ℃ for the second time for 30s, cooling to 20 ℃, aseptically filling, and standing at normal temperature. The final product has a protein content of 2.6% and a viscosity of 580 mPa.s.
Example 2
A preparation method of normal temperature recovery lactic acid milk comprises the following raw material formula:
Figure BDA0002856974420000051
the preparation method comprises the following steps:
(1) preparing reconstituted milk: heating water to 40 deg.C, adding whole milk powder, stirring for dissolving for 30min, cooling to 4 deg.C, standing for 12 hr for hydration to obtain reconstituted milk;
(2) heating the reconstituted milk obtained in the step (1) to 55 ℃, adding other ingredients except the leavening agent, and mixing for 15 min;
(3) heating the material obtained in the step (2) to 65 ℃, homogenizing at 65 ℃ and 18Mpa, and sterilizing at 135 ℃ for 5s to obtain a fermentation substrate;
(4) cooling the fermentation substrate obtained in the step (3) to 39 ℃, adding a leavening agent, fermenting for 4.5h under heat preservation until the final acidity is 70 DEG T, pasteurizing for 80 ℃ for the second time and 10s, cooling to 35 ℃, aseptically filling, and standing at normal temperature. The final product had a protein content of 2.9% and a viscosity of 500 mPa.s.
Example 3
A preparation method of normal temperature recovery lactic acid milk comprises the following raw material formula:
composition (I) Dosage per gram
Reconstituted milk 90.594
Milk protein powder 0.1
Sucrose 7.5
Compound stabilizer (colloid: modified starch ═ 1:3) 1.8
Leaven 0.006
The intelligent method comprises the following steps:
(1) preparing reconstituted milk: heating water to 40 deg.C, adding whole milk powder, stirring for dissolving for 30min, cooling to 4 deg.C, standing for 12 hr for hydration to obtain reconstituted milk;
(2) heating the reconstituted milk obtained in the step (1) to 55 ℃, adding other ingredients except the leavening agent, and mixing for 15 min;
(3) heating the material obtained in the step (2) to 65 ℃, homogenizing at 65 ℃ and 20Mpa, and sterilizing at 95 ℃ for 600s to obtain a fermentation substrate;
(4) cooling the fermentation substrate obtained in the step (3) to 42 ℃, adding a leavening agent, fermenting for 5h under the condition of heat preservation until the final acidity is 70 DEG T, pasteurizing for 80 ℃ for 10s for the second time, cooling to 26 ℃, aseptically filling, and standing at normal temperature. The final product had a protein content of 2.7% and a viscosity of 535 mPa.s.
Comparative example 1-Whole milk powder hydration time 20-30 min according to conventional
The raw material formula is as follows:
composition (I) Dosage per gram
Reconstituted milk 91.99
Sucrose 6
Compound stabilizer (colloid: modified starch ═ 1:3) 2
Leaven 0.01
The preparation method comprises the following steps:
(1) preparing reconstituted milk: heating water to 50 deg.C, adding whole milk powder, stirring for dissolving for 20min, standing for hydrating for 20min to obtain reconstituted milk;
(2) heating the reconstituted milk obtained in the step (1) to 45 ℃, adding other ingredients except the leavening agent, and mixing for 30 min;
(3) heating the material obtained in the step (2) to 60 ℃, homogenizing at 60 ℃ and 25Mpa, and sterilizing at 90 ℃ for 600s to obtain a fermentation substrate;
(4) cooling the fermentation substrate obtained in the step (3) to 43 ℃, adding a leavening agent, fermenting for 9h under the condition of heat preservation until the final acidity is 70 DEG T, pasteurizing for 70 ℃ and 30s for the second time, cooling to 20 ℃, aseptically filling, and standing at normal temperature. The protein content of the final product is 2.6%, and the viscosity is 450mPa. The whole milk powder and water are stirred in the preparation process of the reconstituted milk and are not cooled to 4-6 ℃, the finally obtained product still has the powder feeling of normal-temperature reconstituted milk yoghourt, and the water bleeding is serious in the conventional storage life.
Comparative example 2-Compound stabilizer according to the conventional application range of normal temperature yogurt
The raw material formula is as follows:
composition (I) Dosage per gram
Reconstituted milk 92.69
Sucrose 6
Compound stabilizer (colloid: modified starch ═ 1:3) 1.3
Leaven 380 0.01
The preparation method comprises the following steps:
(1) preparing reconstituted milk: heating water to 50 deg.C, adding whole milk powder, stirring for dissolving for 20min, cooling to 6 deg.C, standing for hydrating for 8 hr to obtain reconstituted milk;
(2) heating the reconstituted milk obtained in the step (1) to 45 ℃, adding other ingredients except the leavening agent, and mixing for 30 min;
(3) heating the material obtained in the step (2) to 60 ℃, homogenizing at 60 ℃ and 25Mpa, and sterilizing at 90 ℃ for 600s to obtain a fermentation substrate;
(4) cooling the fermentation substrate obtained in the step (3) to 43 ℃, adding a leavening agent, fermenting for 6h under the condition of heat preservation until the final acidity is 70 DEG T, pasteurizing for 70 ℃ and 30s for the second time, cooling to 20 ℃, aseptically filling, and standing at normal temperature. The final product had a protein content of 2.6% and a viscosity of 170 mPa.s. The added compound stabilizer is lower than the adding amount of the compound stabilizer, so the viscosity is low, and the requirements of normal-temperature yoghourt products and storage requirements can not be met.
Comparative example 3 too much Compound stabilizer
The raw material formula is as follows:
composition (I) Dosage per gram
Reconstituted milk 91.49
Sucrose 6
Compound stabilizer (colloid: modified starch ═ 1:3) 2.5
Leaven 0.01
The preparation method comprises the following steps:
(1) preparing reconstituted milk: heating water to 50 deg.C, adding whole milk powder, stirring for dissolving for 20min, cooling to 6 deg.C, standing for hydrating for 8 hr to obtain reconstituted milk;
(2) heating the reconstituted milk obtained in the step (1) to 45 ℃, adding other ingredients except the leavening agent, and mixing for 30 min;
(3) heating the material obtained in the step (2) to 60 ℃, homogenizing at 60 ℃ and 25Mpa, and sterilizing at 90 ℃ for 600s to obtain a fermentation substrate;
(4) cooling the fermentation substrate obtained in the step (3) to 43 ℃, adding a leavening agent, and preserving heat for fermentation. The fermentation time exceeded 14 hours and the acidity stayed at 55 ° T. The fermentation end point standard can not be reached. The protein content of the final product is 2.6%.
Comparative example 4 too high proportion of colloid in Compound stabilizer
The raw material formula is as follows:
composition (I) Dosage per gram
Reconstituted milk 91.99
Sucrose 6
Compound stabilizer (colloid: modified starch ═ 1:1) 2
Leaven 0.01
The preparation method comprises the following steps:
(1) preparing reconstituted milk: heating water to 50 deg.C, adding whole milk powder, stirring for dissolving for 20min, cooling to 6 deg.C, standing for hydrating for 8 hr to obtain reconstituted milk;
(2) heating the reconstituted milk obtained in the step (1) to 45 ℃, adding other ingredients except the leavening agent, and mixing for 30 min;
(3) heating the material obtained in the step (2) to 60 ℃, homogenizing at 60 ℃ and 25Mpa, and sterilizing at 90 ℃ for 600s to obtain a fermentation substrate;
(4) cooling the fermentation substrate obtained in the step (3) to 43 ℃, adding a leavening agent, and preserving heat for fermentation. The fermentation time exceeded 14 hours and the acidity stayed at 50 ° T. The fermentation end point standard can not be reached. The protein content of the final product is 2.6%.
Comparative example 5 different starter strains
The raw material formula is as follows:
composition (I) Dosage per gram
Reconstituted milk 89.498
Milk protein powder 0.4
Sucrose 8.5
Compound stabilizer (colloid: modified starch ═ 1:2) 1.6
Leaven (common leaven of existing yoghourt) 0.002
The preparation method comprises the following steps:
(1) preparing reconstituted milk: heating water to 40 deg.C, adding whole milk powder, stirring for dissolving for 30min, cooling to 4 deg.C, standing for 12 hr for hydration to obtain reconstituted milk;
(2) heating the reconstituted milk obtained in the step (1) to 55 ℃, adding other ingredients except the leavening agent, and mixing for 15 min;
(3) heating the material obtained in the step (2) to 65 ℃, homogenizing at 65 ℃ and 18Mpa, and sterilizing at 135 ℃ for 5s to obtain a fermentation substrate;
(4) cooling the fermentation substrate obtained in the step (3) to 39 ℃, adding a leavening agent, and preserving heat for fermentation. The fermentation time exceeded 14 hours and the acidity stayed at 65 ° T. The standard of fermentation end point is not reached. This fermentation time does not meet the requirements of mass production in factories. The protein content of the final product is 2.9%.
Effects of the embodiment
The viscosity, mouthfeel and stability of the products of the examples and the comparative examples are compared according to the storage standard of the products at normal temperature, and the results are as follows:
Figure BDA0002856974420000111
the embodiments of the present invention are merely illustrative and not restrictive, and those skilled in the art can modify the embodiments without inventive contribution as required after reading the present specification, but the present invention is protected by patent law within the scope of the appended claims.

Claims (10)

1. The preparation method of normal-temperature recovery lactic acid milk is characterized by comprising the following preparation raw materials: 6.0-8.5% of cane sugar, 0-0.4% of lactoprotein powder, 1.6-2.0% of compound stabilizer and 0.002-0.01% of leavening agent, and the balance being the restored milk and making up to 100%, wherein the percentages are mass percentages of the total mass of the raw materials; the reconstituted milk is prepared by heating water to 40-50 ℃, adding whole milk powder, stirring and dissolving for 20-30 min, cooling to 4-6 ℃, standing and hydrating for 8-12 hours;
the preparation method of the normal-temperature recovery lactic acid milk comprises the following steps:
(1) heating the reconstituted milk to 45-55 ℃, adding other ingredients except the leavening agent, and mixing for 15-30 min;
(2) homogenizing, sterilizing and cooling the material obtained in the step (1) to obtain a fermentation substrate;
(3) and (3) adding a leaven into the fermentation substrate obtained in the step (2) for fermentation, demulsifying after fermenting for 4.5-6.5 h, performing secondary pasteurization, cooling, and performing aseptic filling.
2. The method for preparing room-temperature reconstituted lactic acid milk according to claim 1, wherein the reconstituted milk is prepared from 12.4% of whole milk powder and 87.6% of water, and the percentages are percentages of the components in the reconstituted milk raw material.
3. The preparation method of the normal-temperature reconstituted lactic acid milk according to claim 1, wherein the milk protein powder is whole milk protein powder or whey protein powder with 70-80% of milk protein content, and the percentage is the mass percentage of the milk protein powder.
4. The preparation method of the normal-temperature recovery lactic acid milk according to claim 1, wherein the compound stabilizer is a colloid stabilizer and modified starch which are compounded according to a ratio of 1: 2-3, and the colloid stabilizer is one or a combination of agar, pectin and gellan gum.
5. The method for preparing room-temperature reconstituted lactic acid milk according to claim 1, wherein the species of the starter culture consists of Streptococcus thermophilus (Streptococcus thermophilus) and Lactobacillus bulgaricus (Lactobacillus bulgaricus).
6. The method for preparing room-temperature-reconstituted lactic acid milk according to claim 1, wherein the homogenization temperature in step (2) is 60-65 ℃, and the homogenization pressure is 18-25 MPa, preferably 18-20 MPa.
7. The preparation method of room-temperature-recovery lactic acid milk according to claim 1, wherein the sterilization in the step (2) is pasteurization or UHT, the sterilization temperature is 90-97 ℃, and the sterilization time is 5-10 min.
8. The preparation method of room-temperature-recovery lactic acid milk according to claim 1, wherein the addition amount of the starter in step (3) is 0.002-0.006%, and the percentage is mass percent of the total mass of the raw materials; and/or the end point of the fermentation is that the titration acidity reaches 70-80 DEG T.
9. The preparation method of normal-temperature reconstituted lactic acid milk according to claim 1, wherein the secondary pasteurization is carried out at a sterilization temperature of 70-80 ℃ for 10-30 s; and/or, cooling to 20-35 ℃ after sterilization.
10. Room temperature reconstituted lactic acid milk obtained by the method for producing room temperature reconstituted lactic acid milk according to any one of claims 1 to 9.
CN202011550417.7A 2020-12-24 2020-12-24 Normal-temperature recovery lactic acid milk and preparation method thereof Pending CN112690333A (en)

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Application publication date: 20210423