CN112680462A - Human papilloma virus 35/HPV 35 type L1/L2 and preparation and application thereof - Google Patents

Abstract

The invention provides a human papilloma virus 35/HPV 35 type L1/L2 and a preparation method and application thereof, and the applicant optimizes encoding genes of L1 protein and L2 protein in pseudovirus, so that the dilution multiple is obviously improved when the pseudovirus is prepared for use, the detection period is short, the sensitivity is high, the repeatability is good, the result interpretation is simple, the detection cost is low, and the method is convenient to apply and popularize.

Description

Human papilloma virus 35/HPV 35 type L1/L2 and preparation and application thereof

Technical Field

The invention relates to the technical field of biology, in particular to human papilloma virus 35/HPV 35 type L1/L2 and preparation and application thereof.

Background

Cervical cancer and pre-cervical lesions are a major problem in women's health worldwide. Clinical, molecular biological, and epidemiological investigations have demonstrated that Human Papillomavirus (HPV) is the major cause of cervical cancer and cervical dysplasia. More than 200 subtypes of HPV are discovered at present, and the high-risk types are classified according to pathogenicity and comprise HPV16, 18, 31, 33, 35, 39, 45 and the like; low risk types include HPV6, 11, etc. HPV is a non-enveloped circular double-stranded DNA virus, the capsid of the HPV is an icosahedral structure consisting of 72 major capsid protein Ll protein pentamers, and 1 virion contains 360 Ll protein monomers. The world health organization international cancer research agency has identified 13 HR-HPVs capable of causing cervical cancer and high-grade premalignant cervical intraepithelial lesions including HPV16, HPV18, HPV31, HPV33, HPV35, HPV39, HPV45, HPV51, HPV52, HPV56, HPV58, HPV59 and HPV 68. Of these, only HPV68 belongs to class 2A in the IARC-induced pain classification, and the remaining 12 belong to class I carcinogens.

Since currently marketed vaccines are up to 9 subtypes, more and more companies are also constantly developing HPV vaccines of more multivalent types (e.g. including HPV 35). According to the guiding principles of the World Health Organization (WHO) on the quality, safety and effectiveness of HPV vaccines and the relevant regulations of the national food and drug administration (CFDA), the key to the success of vaccines is to induce effective humoral immunity and generate neutralizing antibodies with protective effect. Therefore, the determination of the neutralizing activity of the antibody is an important basis for vaccine evaluation and quality control. The development of an effective prophylactic vaccine is of great significance in preventing the transmission of HPV and the occurrence of related diseases. The final aim of HPV vaccine development is to enable the developed vaccine to induce a human body to generate high-titer neutralizing antibodies, so whether the neutralizing antibody detection is an essential and very critical link in the HPV vaccine development process can establish an effective, accurate, sensitive and simple neutralizing antibody evaluation system is very important for the whole HPV vaccine development project. The neutralization assay based on the construction of pseudoviruses is the most straightforward method for detecting antibody activity. The ZsGreen pseudovirus neutralization titer test method can reflect the vaccine efficacy, is a better method, and can detect specific neutralizing antibodies in serum of natural HPV infectors and cervical cancer patients to determine the HPV types infected by the natural HPV infectors and the generated neutralizing antibody level, thereby providing reliable basis for HPV preventive vaccine evaluation and cervical cancer epidemiological research. The existing method for detecting the neutralization titer of the ZsGreen pseudoviruses by adopting microscope observation or flow cytometry has the defects that the sensitivity is low by adopting a microscope method for observation through naked eyes, the operation of the flow cytometer is complex and the inspection equipment is expensive. When the enzyme-linked fluorescent spot analyzer is used for detecting the neutralization titer of the ZsGreen pseudoviruses, the number of fluorescent spots can be accurately counted, and compared with the macroscopic observation of a microscope method, the detection result is more accurate and sensitive. Compared with the flow cytometry detection-block 96-pore plate, the time consumption is about 2-3 hours, the time consumption of the enzyme-linked fluorescence spot analyzer-block 96-pore plate is only a few minutes, the detection time is short, hundreds of 96-pore plates can be counted in one day, and the method has the advantages of high flux and the like.

Disclosure of Invention

In view of the above disadvantages of the prior art, the present invention aims to provide a gene sequence of human papillomavirus type 35L 1/L2 protein, which is used for solving the problems of HPV type 35 pseudovirus preparation and the application of pseudovirus in HPV type 35 samples and antibody titer in the prior art.

The invention conception is as follows: the applicant finds out through experiments that the pseudovirus titer and the experimental sensitivity obtained by optimizing the encoding genes of the human papilloma virus 35 type L1 protein and the L2 protein are higher.

One aspect of the invention provides encoding genes of human papilloma virus 35 type L1 protein and L2 protein, wherein the nucleotide sequence of the encoding gene of the L1 protein is shown as SEQ ID No.4 or SEQ ID No. 5; the nucleotide sequence of the L2 protein coding gene is shown in SEQ ID NO.7 or SEQ ID NO. 8.

In another aspect of the present invention, a recombinant expression vector is provided, wherein the recombinant expression vector contains the above-mentioned coding gene.

In another aspect of the invention, there is provided a host cell comprising or incorporating a recombinant expression vector as described above.

Furthermore, the recombinant expression vector also contains a reporter gene coding sequence.

In another aspect, the present invention provides the use of the genes encoding human papillomavirus type 35L1 protein and L2 protein, recombinant expression vectors, or host cells in the preparation of human papillomavirus type 35 pseudoviruses.

Another aspect of the present invention provides a method for constructing human papillomavirus type 35 pseudovirus, which comprises using the sequence shown as nucleotide sequence SEQ ID NO.4 or SEQ ID NO.5 to encode L1 protein and the sequence shown as nucleotide sequence SEQ ID NO.7 or SEQ ID NO.8 to encode L2 protein.

Knowing the gene encoding L1, one skilled in the art can construct the human papillomavirus type 35 pseudovirus according to the prior knowledge, and the specific operation method comprises the following steps:

1) constructing a recombinant expression vector containing the coding gene of the human papilloma virus 35 type L1 protein and a recombinant expression vector containing the coding gene of the human papilloma virus 35 type L2 protein

2) Culturing and expressing the two expression vector host cells;

3) the cells are lysed and the protein of interest is collected.

Furthermore, the two recombinant expression vectors also contain a reporter protein expression gene.

Furthermore, the reporter protein is a fluorescent protein, such as any one or more of green fluorescent protein, red fluorescent protein, yellow fluorescent protein and blue fluorescent protein.

As described above, the gene sequences of the human papillomavirus type 35L1 protein and the L2 protein of the present invention have the following beneficial effects:

the invention adopts the optimized human papillomavirus 35L1 protein and L2 protein coding genes to prepare the HPV35 pseudovirus, the use dilution multiple of the pseudovirus is obviously improved, the detection period is short, the sensitivity is high, the repeatability is good, the result interpretation is simple, the detection cost is low, and the method is convenient to apply and popularize.

Drawings

FIG. 1 is a graph showing the relationship between the fluorescent point of HPV type 35 pseudovirus prepared from HPV 35L1+ HPV 35L2 expression plasmid before gene sequence optimization and pseudovirus dilution factor

FIG. 2 is a diagram showing the relationship between the fluorescent point of HPV35 pseudovirus and the dilution factor of pseudovirus prepared from HPV 35L1-op1+ HPV 35L2-op1 expression plasmid after gene sequence optimization.

FIG. 3 is a diagram showing the relationship between the fluorescent point of HPV35 pseudovirus and the dilution factor of pseudovirus prepared from HPV 35L1-op2+ HPV 35L2-op2 expression plasmid after gene sequence optimization.

FIG. 4 fluorescent dot count chart of HPV type 35 pseudovirus used in determination of serum neutralizing antibody titer.

Detailed Description

The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention. It is to be understood that the processing equipment or apparatus not specifically identified in the following examples is conventional in the art. Furthermore, it is to be understood that one or more method steps mentioned in the present invention does not exclude that other method steps may also be present before or after the combined steps or that other method steps may also be inserted between these explicitly mentioned steps, unless otherwise indicated; it is also to be understood that a combined connection between one or more devices/apparatus as referred to in the present application does not exclude that further devices/apparatus may be present before or after the combined device/apparatus or that further devices/apparatus may be interposed between two devices/apparatus explicitly referred to, unless otherwise indicated. Moreover, unless otherwise indicated, the numbering of the various method steps is merely a convenient tool for identifying the various method steps, and is not intended to limit the order in which the method steps are arranged or the scope of the invention in which the invention may be practiced, and changes or modifications in the relative relationship may be made without substantially changing the technical content.

The above examples are intended to illustrate the disclosed embodiments of the invention and are not to be construed as limiting the invention. In addition, various modifications of the methods and compositions set forth herein, as well as variations of the methods and compositions of the present invention, will be apparent to those skilled in the art without departing from the scope and spirit of the invention. While the invention has been specifically described in connection with various specific preferred embodiments thereof, it should be understood that the invention should not be unduly limited to such specific embodiments. Indeed, various modifications of the above-described embodiments which are obvious to those skilled in the art to which the invention pertains are intended to be covered by the scope of the present invention.

Example 1 optimization of HPV 35L1 protein and HPV 35L2 protein-encoding Gene

HPV 35L1 protein and HPV 35L2 protein encoding genes are optimized based on multiple dimensions such as codon usage frequency, RNA secondary structure, GC content and the like. The amino acid sequence of the HPV 35L1 protein is shown as SEQ ID NO.1 (GenBank accession number: CAA52566.1), and the amino acid sequence of the HPV 35L2 protein is shown as SEQ ID NO.2 (GenBank accession number: CAA 52565.1). The encoding gene of the HPV 35L1 protein before optimization is SEQ ID NO.3, and the encoding gene of the optimized HPV 35L1 protein is SEQ ID NO.4(HPV 35L1-op1) and SEQ ID NO.5(HPV 35L1-op 2). The encoding gene of the HPV 35L2 protein before optimization is SEQ ID NO.6, and the encoding gene of the optimized HPV 35L2 protein is SEQ ID NO.7(HPV 35L2-op1) and SEQ ID NO.8(HPV 35L2-op 2).

SEQ ID NO.1(HPV 35L1 protein amino acid sequence)

MSLWRSNEATVYLPPVSVSKVVSTDEYVTRTNIYYHAGSSRLLAVGHPYYAIKKQDSNKI AVPKVSGLQYRVFRVKLPDPNKFGFPDTSFYDPASQRLVWACTGVEVGRGQPLGVGISGH PLLNKLDDTENSNKYVGNSGTDNRECISMDYKQTQLCLIGCRPPIGEHWGKGTPCNANQV KAGECPPLELLNTVLQDGDMVDTGFGAMDFTTLQANKSDVPLDICSSICKYPDYLKMVSE PYGDMLFFYLRREQMFVRHLFNRAGTVGETVPADLYIKGTTGTLPSTSYFPTPSGSMVTSD AQIFNKPYWLQRAQGHNNGICWSNQLFVTVVDTTRSTNMSVCSAVSSSDSTYKNDNFKEY LRHGEEYDLQFIFQLCKITLTADVMTYIHSMNPSILEDWNFGLTPPPSGTLEDTYRYVTSQA VTCQKPSAPKPKDDPLKNYTFWEVDLKEKFSADLDQFPLGRKFLLQAGLKARPNFRLGKR AAPASTSKKSSTKRRKVKS

SEQ ID NO.2(HPV 35L2 protein amino acid sequence)

MRHKRSTKRVKRASATQLYRTCKAAGTCPPDVIPKVEGNTVADQILKYGSMAVFFGGLGI GSGSGTGGRSGYVPLGTTPPTAATNIPIRPPVTVESIPLDTIGPLDSSIVSLVEETSFIESGAPV VTPRVPPTTGFTITTSTDTTPAILDVTSISTHDNPTFTDPSVLHPPTPAETSGHFVLSSSSISTH NYEEIPMDTFIVSTDSNNITNSTPIPGSRPTTRLGLYSKGTQQVKVVDPAFMTSPAKLITYDN PAYEGLNPDTTLQFEHEDISLAPDPDFMDIIALHRPALTSRKGTIRYSRVGNKRTMHTRSGK AIGARVHYYQDLSSITEDIELQPLQHVPSSLPHTTVSTSLNDGMFDIYAPIDTEEDIIFSASSN NTLYTTSNTAYVPSNTTIPLSSGYDIPITAGPDIVFNSNTITNTVLPVPTGPIYSIIADGGDFYL HPSYYLLKRRRKRIPYFFADVSVAV

SEQ ID NO.3 (HPV 35L1 protein coding gene before optimization)

ATGTCTCTGTGGAGATCCAACGAGGCCACTGTCTACCTGCCTCCAGTTTCGGTGTCTAAG GTTGTGTCCACGGACGAGTACGTCACTAGAACCAACATCTACTACCACGCAGGTTCCTC TAGACTCCTGGCTGTTGGTCACCCTTACTATGCCATTAAGAAGCAGGACTCGAACAAGA TCGCCGTCCCAAAGGTTTCTGGCTTGCAGTACAGAGTGTTCAGAGTTAAGCTGCCAGAC CCTAACAAGTTCGGATTTCCAGACACCTCCTTCTACGACCCTGCTTCCCAGAGATTGGTT TGGGCATGCACTGGAGTCGAGGTGGGCAGAGGTCAGCCATTGGGAGTTGGTATCTCTGG CCACCCTTTGCTGAACAAGCTCGACGATACCGAGAACTCCAACAAGTACGTTGGCAACT CTGGAACCGACAACAGAGAGTGCATCTCGATGGACTACAAGCAGACCCAGTTGTGTCT CATCGGATGCAGACCACCTATTGGTGAACATTGGGGAAAGGGCACCCCTTGCAACGCCA ACCAGGTCAAGGCCGGAGAGTGTCCTCCATTGGAGCTTCTGAACACTGTTCTCCAAGAT GGTGACATGGTTGACACCGGCTTTGGTGCTATGGACTTCACGACCTTGCAGGCCAACAA GTCCGACGTGCCACTTGACATCTGTTCTTCCATTTGCAAGTACCCTGATTACCTGAAGAT GGTTTCGGAGCCATACGGAGACATGCTCTTCTTTTACCTGAGAAGAGAGCAGATGTTCG TGAGACACTTGTTCAACAGAGCAGGAACTGTTGGTGAAACGGTCCCTGCTGACCTGTA CATCAAGGGCACCACTGGTACGTTGCCATCTACCTCGTACTTCCCTACTCCATCTGGTTC GATGGTCACCTCCGATGCCCAGATCTTCAACAAGCCATACTGGTTGCAGAGAGCCCAGG GACACAACAATGGCATTTGCTGGTCCAACCAGCTGTTCGTGACCGTCGTTGACACTACG AGATCCACCAACATGTCGGTGTGTTCTGCAGTCAGCTCTTCCGACTCGACCTACAAGAA CGACAACTTCAAGGAGTACCTCAGACACGGTGAAGAGTACGACCTGCAATTCATCTTCC AGTTGTGCAAGATCACCCTGACTGCTGACGTTATGACGTACATTCACTCCATGAACCCTT CGATCCTGGAGGACTGGAACTTCGGTCTTACTCCACCTCCATCTGGCACCTTGGAGGAC ACTTACAGATATGTCACCTCCCAAGCTGTTACGTGTCAGAAGCCTTCGGCCCCAAAGCCT AAGGACGATCCACTGAAGAACTACACCTTCTGGGAGGTTGACCTGAAGGAGAAGTTCT CCGCAGACCTCGACCAGTTCCCATTGGGCAGAAAGTTCCTGCTCCAAGCTGGATTGAAG GCCAGACCTAACTTCAGACTTGGCAAGAGAGCCGCTCCAGCATCTACCTCTAAGAAATC GTCCACGAAGCGCAGAAAGGTGAAGTCG

SEQ ID NO.4 (optimized HPV 35L1 protein coding gene HPV 35L1-op1)

ATGAGCCTGTGGAGAAGCAACGAGGCCACCGTGTACCTGCCCCCGGTGAGCGTGAGCA AGGTGGTGAGCACCGACGAGTACGTGACAAGAACCAACATCTACTACCACGCCGGCAG CAGCAGACTGCTGGCCGTGGGCCACCCCTACTACGCCATCAAGAAGCAAGACAGCAAC AAGATCGCCGTGCCCAAGGTGAGCGGCCTGCAGTACAGAGTGTTCAGAGTGAAGCTGC CCGACCCCAACAAGTTCGGCTTCCCCGACACTAGCTTCTATGACCCGGCTAGCCAAAGA CTGGTGTGGGCGTGTACGGGCGTGGAGGTAGGTAGAGGGCAGCCACTGGGCGTGGGCA TCAGCGGCCACCCCCTGCTGAACAAGCTGGACGACACCGAGAACAGCAACAAGTACGT GGGCAACAGCGGCACCGACAACAGAGAGTGCATCAGCATGGACTACAAGCAGACACA GCTGTGCCTGATCGGCTGCAGACCCCCCATCGGCGAGCACTGGGGCAAAGGCACCCCG TGTAACGCTAACCAAGTCAAGGCGGGAGAGTGCCCCCCCCTGGAGCTGCTGAACACCG TGCTGCAAGACGGCGACATGGTGGACACCGGCTTCGGCGCCATGGACTTCACCACCCT GCAAGCCAACAAGAGCGACGTGCCCCTGGACATCTGCAGCAGCATCTGCAAGTACCCC GACTACCTGAAGATGGTGAGCGAGCCCTACGGCGACATGCTGTTCTTCTACCTGAGAAG AGAGCAGATGTTCGTGAGACACCTGTTCAACAGAGCCGGCACCGTGGGCGAGACCGTG CCCGCCGACCTGTACATCAAGGGCACCACCGGCACCCTGCCTAGCACAAGCTACTTCCC CACGCCTAGCGGCAGCATGGTGACAAGCGACGCTCAGATCTTCAACAAGCCCTACTGGC TGCAGAGAGCCCAAGGCCACAACAACGGCATCTGCTGGAGCAATCAGCTGTTCGTGAC CGTGGTGGACACCACAAGAAGCACCAACATGAGCGTGTGCAGCGCCGTGAGCAGCAG CGACAGCACCTACAAGAACGACAACTTCAAGGAGTACCTGAGACACGGCGAGGAGTAC GACCTGCAGTTCATCTTTCAGCTGTGCAAGATCACCCTGACCGCCGACGTGATGACCTA CATCCACAGCATGAACCCTAGCATCCTGGAGGACTGGAACTTCGGCCTGACCCCCCCTC CTAGCGGCACCCTGGAGGACACCTACAGATACGTGACAAGCCAAGCCGTGACCTGTCA GAAGCCTAGCGCCCCCAAGCCCAAGGACGACCCCCTGAAGAACTACACCTTCTGGGAG GTGGACCTGAAGGAGAAGTTCAGCGCCGACCTGGATCAGTTCCCCCTGGGCAGAAAGT TCCTGCTGCAAGCCGGCCTGAAGGCTAGACCCAACTTCAGACTGGGCAAGAGAGCCGC CCCCGCTAGCACAAGCAAGAAGAGCAGCACCAAGAGAAGAAAGGTGAAGAGC

SEQ ID NO.5 (optimized HPV 35L1 protein coding gene HPV 35L1-op2)

ATGAGCCTGTGGAGAAGCAATGAGGCCACAGTGTACCTGCCCCCTGTGTCTGTGAGCAA GGTGGTGAGCACAGATGAGTATGTGACAAGAACCAACATCTACTACCATGCTGGCAGCA GCAGACTGCTGGCTGTGGGCCACCCCTACTATGCCATCAAGAAGCAAGACAGCAACAA GATTGCTGTGCCCAAGGTGTCTGGCCTGCAGTACAGAGTGTTCAGAGTGAAGCTGCCTG ACCCCAACAAGTTTGGCTTCCCTGACACTAGCTTCTATGACCCTGCTAGCCAAAGACTG GTGTGGGCCTGTACTGGGGTGGAGGTAGGTAGAGGGCAGCCACTGGGGGTGGGCATCT CTGGCCACCCCCTGCTGAACAAGCTGGATGACACAGAGAACAGCAACAAGTATGTGGG CAACTCTGGCACAGACAACAGAGAGTGCATCAGCATGGACTACAAGCAGACACAGCTG TGCCTGATTGGCTGCAGACCCCCCATTGGGGAGCACTGGGGCAAAGGCACCCCCTGTAA TGCCAACCAAGTCAAGGCTGGAGAGTGCCCCCCCCTGGAGCTGCTGAACACAGTGCTG CAAGATGGGGACATGGTGGACACTGGCTTTGGGGCCATGGACTTCACCACCCTGCAAG CCAACAAGTCTGATGTGCCCCTGGACATCTGCAGCAGCATCTGCAAGTACCCTGACTAC CTGAAGATGGTGTCTGAGCCCTATGGGGACATGCTGTTCTTCTACCTGAGAAGAGAGCA GATGTTTGTGAGACACCTGTTCAACAGAGCTGGCACAGTGGGGGAGACAGTGCCTGCT GACCTGTACATCAAGGGCACCACTGGCACCCTGCCTAGCACAAGCTACTTCCCCACCCC CTCTGGCAGCATGGTGACCTCTGATGCTCAGATCTTCAACAAGCCCTACTGGCTGCAGA GAGCCCAAGGCCACAACAATGGCATCTGCTGGAGCAATCAGCTGTTTGTGACAGTGGT GGACACCACAAGAAGCACCAACATGTCTGTGTGCTCTGCTGTGAGCAGCTCTGACAGC ACCTACAAGAATGACAACTTCAAGGAGTACCTGAGACATGGGGAGGAGTATGACCTGC AGTTCATCTTTCAGCTGTGCAAGATCACCCTGACAGCTGATGTGATGACCTACATCCACA GCATGAACCCTAGCATCCTGGAGGACTGGAACTTTGGCCTGACCCCCCCTCCCTCTGGC ACCCTGGAGGACACCTACAGATATGTGACAAGCCAAGCTGTGACCTGTCAGAAGCCCTC TGCCCCCAAGCCCAAGGATGACCCCCTGAAGAACTACACCTTCTGGGAGGTGGACCTG AAGGAGAAGTTCTCTGCTGACCTGGATCAGTTCCCCCTGGGCAGAAAGTTCCTGCTGCA AGCTGGCCTGAAGGCTAGACCCAACTTCAGACTGGGCAAGAGAGCTGCCCCTGCTAGC ACAAGCAAGAAGAGCAGCACCAAGAGAAGAAAGGTGAAGAGC

SEQ ID NO.6 (HPV 35L2 protein coding gene before optimization)

ATGCGCCACAAGCGGTCCACCAAGAGAGTGAAGAGGGCCTCTGCCACACAGCTGTACA GAACCTGCAAGGCAGCAGGAACATGTCCACCTGACGTGATCCCCAAGGTGGAGGGCA ACACCGTGGCCGATCAGATCCTGAAGTACGGCTCTATGGCCGTGTTCTTTGGCGGCCTG GGCATCGGATCTGGAAGCGGAACCGGCGGCCGGAGCGGCTATGTGCCACTGGGAACC ACACCACCAACAGCAGCAACCAATATCCCCATCCGGCCTCCAGTGACAGTGGAGTCCA TCCCCCTGGACACCATCGGCCCTCTGGATAGCTCCATCGTGTCTCTGGTGGAGGAGACA TCCTTCATCGAGTCTGGAGCACCTGTGGTGACCCCAAGGGTGCCACCTACCACAGGCTT TACAATCACCACATCCACCGACACCACACCTGCCATCCTGGATGTGACATCCATCTCTA CCCACGACAACCCAACCTTCACAGATCCATCTGTGCTGCACCCACCAACACCAGCAGA GACCAGCGGCCACTTTGTGCTGTCTAGCTCCTCTATCTCCACCCACAACTACGAGGAGA TCCCTATGGACACATTCATCGTGTCTACCGATAGCAACAATATCACAAATAGCACCCCT ATCCCAGGCTCCAGACCTACCACAAGGCTGGGCCTGTATAGCAAGGGCACCCAGCAGG TGAAGGTGGTGGACCCAGCCTTTATGACATCCCCCGCCAAGCTGATCACCTACGATAA CCCCGCCTATGAGGGCCTGAATCCAGACACCACACTGCAGTTCGAGCACGAGGATATC TCCCTGGCCCCCGACCCTGACTTCATGGACATCATCGCCCTGCACAGACCAGCCCTGAC AAGCAGGAAGGGCACCATCCGCTACTCCCGGGTGGGCAACAAGCGCACAATGCACAC CCGGAGCGGCAAGGCAATCGGAGCAAGGGTGCACTACTATCAGGACCTGAGCTCCATC ACCGAGGATATCGAGCTGCAGCCACTGCAGCACGTGCCCTCTAGCCTGCCTCACACCA CAGTGAGCACATCCCTGAATGACGGCATGTTCGATATCTACGCCCCCATCGACACCGA GGAGGATATCATCTTTTCCGCCTCCTCTAACAATACACTGTACACCACATCTAACACCG CCTATGTGCCTAGCAATACCACAATCCCACTGAGCTCCGGCTATGACATCCCTATCACA GCCGGCCCAGATATCGTGTTCAACAGCAATACCATCACAAATACCGTGCTGCCAGTGC CAACCGGACCAATCTACTCTATCATCGCAGACGGAGGCGACTTCTACCTGCACCCTAG CTACTATCTGCTGAAGCGGAGAAGGAAGCGGATCCCATATTTCTTTGCCGACGTGAGC GTGGCCGTG

SEQ ID NO.7 (optimized HPV 35L2 protein coding gene HPV 35L2-op1)

ATGAGACACAAGAGAAGCACCAAGAGAGTGAAGAGAGCTAGCGCCACACAGCTGTAC AGAACCTGCAAGGCCGCCGGCACCTGCCCCCCCGACGTGATCCCCAAGGTGGAGGGCA ACACCGTGGCCGATCAGATCCTGAAGTATGGCAGCATGGCTGTCTTCTTCGGCGGCCTG GGTATCGGCAGCGGAAGCGGCACCGGCGGCAGAAGTGGGTATGTGCCCCTTGGTACGA CACCCCCCACCGCCGCCACCAACATCCCCATCAGACCCCCCGTGACCGTGGAGAGCAT CCCCCTGGACACCATCGGCCCCCTGGACAGCAGCATCGTGAGCCTGGTGGAGGAGACT AGCTTCATCGAGAGCGGCGCCCCCGTGGTGACCCCTAGAGTGCCCCCCACCACCGGCT TCACCATCACCACAAGCACGGACACGACACCCGCCATCCTGGACGTGACCTCCATCAG CACTCACGATAACCCTACCTTTACCGACCCTAGCGTTCTGCATCCCCCCACGCCCGCCG AGACAAGCGGCCACTTCGTGCTGAGCAGCAGCAGCATCAGCACCCACAACTATGAGGA GATCCCCATGGACACCTTCATCGTGAGCACCGACAGCAACAACATCACAAACTCCACA CCCATTCCCGGCAGCAGACCCACCACAAGACTGGGCCTGTACAGCAAGGGCACACAGC AAGTGAAGGTGGTGGACCCCGCCTTCATGACAAGCCCCGCCAAGCTGATCACCTACGA CAACCCCGCCTACGAGGGCCTGAACCCCGACACAACGCTGCAGTTCGAGCACGAGGAC ATCAGCCTGGCCCCCGACCCCGACTTCATGGACATCATCGCCCTGCACAGACCCGCCCT GACAAGCAGAAAGGGCACCATCAGATACAGCAGAGTGGGCAACAAGAGAACCATGCA CACAAGAAGCGGCAAGGCCATCGGCGCTAGAGTGCACTACTACCAAGACCTGAGCAG CATCACCGAGGACATCGAGCTGCAGCCCCTGCAGCACGTTCCCTCGTCCCTGCCCCACA CCACCGTGAGCACAAGCCTGAACGACGGCATGTTCGACATCTACGCCCCCATCGACAC CGAGGAGGACATCATCTTCAGCGCTAGCAGCAACAACACCCTGTACACCACAAGCAAC ACCGCCTACGTGCCTAGCAACACCACCATCCCCCTGAGCAGCGGCTACGACATCCCCA TAACTGCCGGCCCCGACATCGTGTTCAACAGCAACACCATCACCAACACCGTGCTGCC CGTGCCCACCGGCCCCATCTACAGCATCATCGCCGACGGCGGCGACTTCTACCTGCACC CTAGCTACTACCTGCTGAAGAGAAGAAGAAAGAGAATCCCCTACTTCTTCGCCGACGT GAGCGTGGCCGTG

SEQ ID NO.8 (optimized HPV 35L2 protein coding gene HPV 35L2-op2)

ATGAGACACAAGAGAAGCACCAAGAGAGTGAAGAGAGCCTCTGCCACACAGCTGTAC AGAACCTGCAAGGCTGCTGGCACCTGCCCCCCTGATGTGATCCCCAAGGTGGAGGGCA ACACAGTGGCTGATCAGATCCTGAAGTATGGCAGCATGGCTGTCTTCTTTGGGGGCCTG GGTATTGGCTCTGGCTCTGGCACTGGGGGCAGAAGTGGGTATGTGCCCCTTGGTACCAC ACCCCCCACAGCTGCCACCAACATCCCCATCAGACCCCCTGTGACAGTGGAGAGCATCC CCCTGGACACCATTGGCCCCCTGGACAGCAGCATTGTGAGCCTGGTGGAGGAGACTAG CTTCATTGAGTCTGGGGCCCCTGTGGTGACCCCTAGAGTGCCCCCCACCACTGGCTTCA CCATCACCACAAGCACAGACACCACACCTGCCATCCTGGATGTGACCTCCATCAGCACT CATGACAACCCTACCTTTACAGACCCCTCTGTGCTGCATCCCCCCACCCCTGCTGAGACC TCTGGCCACTTTGTGCTGAGCAGCAGCAGCATCAGCACCCACAACTATGAGGAGATCCC CATGGACACCTTCATTGTGAGCACAGACAGCAACAACATCACAAACTCCACACCCATTC CTGGCAGCAGACCCACCACAAGACTGGGCCTGTACAGCAAGGGCACACAGCAAGTGA AGGTGGTGGACCCTGCCTTCATGACAAGCCCTGCCAAGCTGATCACCTATGACAACCCT GCCTATGAGGGCCTGAACCCTGACACAACCCTGCAGTTTGAGCATGAGGACATCAGCCT GGCCCCTGACCCTGACTTCATGGACATCATTGCCCTGCACAGACCTGCCCTGACAAGCA GAAAGGGCACCATCAGATACAGCAGAGTGGGCAACAAGAGAACCATGCACACAAGATC TGGCAAGGCCATTGGGGCTAGAGTGCACTACTACCAAGACCTGAGCAGCATCACAGAG GACATTGAGCTGCAGCCCCTGCAGCATGTGCCTAGCTCCCTGCCCCACACCACAGTGAG CACAAGCCTGAATGATGGCATGTTTGACATCTATGCCCCCATTGACACAGAGGAGGACA TCATCTTCTCTGCTAGCAGCAACAACACCCTGTACACCACAAGCAACACAGCCTATGTG CCTAGCAACACCACCATCCCCCTGAGCTCTGGCTATGACATCCCCATAACTGCTGGCCCT GACATTGTGTTCAACAGCAACACCATCACCAACACAGTGCTGCCTGTGCCCACTGGCCC CATCTACAGCATCATTGCTGATGGGGGGGACTTCTACCTGCACCCTAGCTACTACCTGCT GAAGAGAAGAAGAAAGAGAATCCCCTACTTCTTTGCTGATGTGTCTGTGGCTGTG

Example 2 construction and preparation of HPV type 35 pseudovirus

Step 1: HPV 35L1, HPV 35L1-op1, HPV 35L1-op2, HPV 35L2, HPV 35L2-op1, HPV 35L2-op2 and EGFP (fluorescent protein reporter) genes are inserted into pCDNA3.1 plasmids to respectively obtain expression plasmids 35L1-pCDNA3.1(SEQ ID NO.9), 35L1-op1-pCDNA3.1(SEQ ID NO.10), 35L1-op2-pCDNA3.1 (SEQ ID NO.11), 35L2-pCDNA3.1(SEQ ID NO.12), 35L 2-1-pCDNA3.1 (SEQ ID NO.13), 35L2-op2-pCDNA3.1(SEQ ID NO.14) and EGFP-pCDNA3.1(SEQ ID NO. 15).

Expression plasmid 35L1-pCDNA3.1 sequence (containing the pre-optimized gene HPV 35L1), SEQ ID NO. 9:

GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATG CCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCG CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTG CTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACAT TGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATAT GGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACC CCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC ATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGT ATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATT ATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCAT CGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGA CTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACC AAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGC GGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACC CACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTA GCGTTTAAACGGGCCCTCTAGACTCGAGCGGCCGCCACCATGTCTCTGTGGAGATCCAA CGAGGCCACTGTCTACCTGCCTCCAGTTTCGGTGTCTAAGGTTGTGTCCACGGACGAGT ACGTCACTAGAACCAACATCTACTACCACGCAGGTTCCTCTAGACTCCTGGCTGTTGGTC ACCCTTACTATGCCATTAAGAAGCAGGACTCGAACAAGATCGCCGTCCCAAAGGTTTCT GGCTTGCAGTACAGAGTGTTCAGAGTTAAGCTGCCAGACCCTAACAAGTTCGGATTTCC AGACACCTCCTTCTACGACCCTGCTTCCCAGAGATTGGTTTGGGCATGCACTGGAGTCG AGGTGGGCAGAGGTCAGCCATTGGGAGTTGGTATCTCTGGCCACCCTTTGCTGAACAAG CTCGACGATACCGAGAACTCCAACAAGTACGTTGGCAACTCTGGAACCGACAACAGAG AGTGCATCTCGATGGACTACAAGCAGACCCAGTTGTGTCTCATCGGATGCAGACCACCT ATTGGTGAACATTGGGGAAAGGGCACCCCTTGCAACGCCAACCAGGTCAAGGCCGGAG AGTGTCCTCCATTGGAGCTTCTGAACACTGTTCTCCAAGATGGTGACATGGTTGACACC GGCTTTGGTGCTATGGACTTCACGACCTTGCAGGCCAACAAGTCCGACGTGCCACTTGA CATCTGTTCTTCCATTTGCAAGTACCCTGATTACCTGAAGATGGTTTCGGAGCCATACGG AGACATGCTCTTCTTTTACCTGAGAAGAGAGCAGATGTTCGTGAGACACTTGTTCAACA GAGCAGGAACTGTTGGTGAAACGGTCCCTGCTGACCTGTACATCAAGGGCACCACTGG TACGTTGCCATCTACCTCGTACTTCCCTACTCCATCTGGTTCGATGGTCACCTCCGATGCC CAGATCTTCAACAAGCCATACTGGTTGCAGAGAGCCCAGGGACACAACAATGGCATTTG CTGGTCCAACCAGCTGTTCGTGACCGTCGTTGACACTACGAGATCCACCAACATGTCGG TGTGTTCTGCAGTCAGCTCTTCCGACTCGACCTACAAGAACGACAACTTCAAGGAGTAC CTCAGACACGGTGAAGAGTACGACCTGCAATTCATCTTCCAGTTGTGCAAGATCACCCT GACTGCTGACGTTATGACGTACATTCACTCCATGAACCCTTCGATCCTGGAGGACTGGA ACTTCGGTCTTACTCCACCTCCATCTGGCACCTTGGAGGACACTTACAGATATGTCACCT CCCAAGCTGTTACGTGTCAGAAGCCTTCGGCCCCAAAGCCTAAGGACGATCCACTGAA GAACTACACCTTCTGGGAGGTTGACCTGAAGGAGAAGTTCTCCGCAGACCTCGACCAG TTCCCATTGGGCAGAAAGTTCCTGCTCCAAGCTGGATTGAAGGCCAGACCTAACTTCAG ACTTGGCAAGAGAGCCGCTCCAGCATCTACCTCTAAGAAATCGTCCACGAAGCGCAGA AAGGTGAAGTCGTAATAGAAGCTTAAGTTTAAACCGCTGATCAGCCTCGACTGTGCCTT CTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTG CCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGT GTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGA CAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCA GCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGT GTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTT CGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCG GGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGA TTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGAC GTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACC CTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAA AATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAG GGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAAT TAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAA GCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCC TAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGC AGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTG GAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATC AAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCT CCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTG CTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGA CCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCT GGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGG GACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCT GCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCT ACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGA AGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCC GAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCC ATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCG ACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGAT ATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGC CGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGG ACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGA TTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCT GGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTA TTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCAT TTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGT ATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGA AATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGC CTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTT CCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGA GGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTC GTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGA ATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAA CCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCA GGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGG ATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAG GTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTA GGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGT ATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTG ATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGCAAGCAGCAGATTACGC GCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAG TGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCAC CTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACT TGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTC GTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTAC CATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTAT CAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATC CGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAA TAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGG TATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTT GTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCG CAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGT AAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCG GCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAA CTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTA CCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCT TTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAA AGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTG AAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAAT AAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTC

expression plasmid 35L1-op1-pCDNA3.1 sequence (containing optimized gene HPV 35L1-op1), SEQ ID NO. 10:

GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATG CCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCG CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTG CTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACAT TGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATAT GGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACC CCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC ATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGT ATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATT ATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCAT CGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGA CTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACC AAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGC GGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACC CACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTA GCGTTTAAACGGGCCCTCTAGACTCGAGCGGCCGCCACCATGAGCCTGTGGAGAAGCA ACGAGGCCACCGTGTACCTGCCCCCGGTGAGCGTGAGCAAGGTGGTGAGCACCGACGA GTACGTGACAAGAACCAACATCTACTACCACGCCGGCAGCAGCAGACTGCTGGCCGTG GGCCACCCCTACTACGCCATCAAGAAGCAAGACAGCAACAAGATCGCCGTGCCCAAGG TGAGCGGCCTGCAGTACAGAGTGTTCAGAGTGAAGCTGCCCGACCCCAACAAGTTCGG CTTCCCCGACACTAGCTTCTATGACCCGGCTAGCCAAAGACTGGTGTGGGCGTGTACGG GCGTGGAGGTAGGTAGAGGGCAGCCACTGGGCGTGGGCATCAGCGGCCACCCCCTGCT GAACAAGCTGGACGACACCGAGAACAGCAACAAGTACGTGGGCAACAGCGGCACCGA CAACAGAGAGTGCATCAGCATGGACTACAAGCAGACACAGCTGTGCCTGATCGGCTGC AGACCCCCCATCGGCGAGCACTGGGGCAAAGGCACCCCGTGTAACGCTAACCAAGTCA AGGCGGGAGAGTGCCCCCCCCTGGAGCTGCTGAACACCGTGCTGCAAGACGGCGACAT GGTGGACACCGGCTTCGGCGCCATGGACTTCACCACCCTGCAAGCCAACAAGAGCGAC GTGCCCCTGGACATCTGCAGCAGCATCTGCAAGTACCCCGACTACCTGAAGATGGTGAG CGAGCCCTACGGCGACATGCTGTTCTTCTACCTGAGAAGAGAGCAGATGTTCGTGAGAC ACCTGTTCAACAGAGCCGGCACCGTGGGCGAGACCGTGCCCGCCGACCTGTACATCAA GGGCACCACCGGCACCCTGCCTAGCACAAGCTACTTCCCCACGCCTAGCGGCAGCATGG TGACAAGCGACGCTCAGATCTTCAACAAGCCCTACTGGCTGCAGAGAGCCCAAGGCCA CAACAACGGCATCTGCTGGAGCAATCAGCTGTTCGTGACCGTGGTGGACACCACAAGA AGCACCAACATGAGCGTGTGCAGCGCCGTGAGCAGCAGCGACAGCACCTACAAGAACG ACAACTTCAAGGAGTACCTGAGACACGGCGAGGAGTACGACCTGCAGTTCATCTTTCA GCTGTGCAAGATCACCCTGACCGCCGACGTGATGACCTACATCCACAGCATGAACCCTA GCATCCTGGAGGACTGGAACTTCGGCCTGACCCCCCCTCCTAGCGGCACCCTGGAGGAC ACCTACAGATACGTGACAAGCCAAGCCGTGACCTGTCAGAAGCCTAGCGCCCCCAAGC CCAAGGACGACCCCCTGAAGAACTACACCTTCTGGGAGGTGGACCTGAAGGAGAAGTT CAGCGCCGACCTGGATCAGTTCCCCCTGGGCAGAAAGTTCCTGCTGCAAGCCGGCCTG AAGGCTAGACCCAACTTCAGACTGGGCAAGAGAGCCGCCCCCGCTAGCACAAGCAAGA AGAGCAGCACCAAGAGAAGAAAGGTGAAGAGCTAATAGAAGCTTAAGTTTAAACCGCT GATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGC CTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTG CATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGC AAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGG CTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGC GGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAG CGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTT CCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCAC CTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATA GACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCA AACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCG ATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCT GTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGT ATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCC AGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCC TAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCT GACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGA AGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGT ATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAA GATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTG GGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGG CGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGA GGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACG TTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCT CCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCG GCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCG AGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGA GCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGAC GGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAAT GGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGA CATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCT TCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCT TGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCA ACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAA TCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTC TTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCA CAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCAT CAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGG TCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCC GGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGC GTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAAT CGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCA CTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCG GTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCT CCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCG ACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTT CCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTT TCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGC TGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTT GAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGAT TAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG GCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTT TGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTC TACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT TATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTA AAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTAT CTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT ACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACG CTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGA AGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGA GTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTG GTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGA GTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT GTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCT CTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCA TTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAAT ACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCG AAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCAC CCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGA AGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATAC TCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATA TTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG CCACCTGACGTC

expression plasmid 35L1-op2-pCDNA3.1 sequence (containing optimized gene HPV 35L1-op2), SEQ ID NO. 11:

GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATG CCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCG CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTG CTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACAT TGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATAT GGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACC CCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC ATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGT ATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATT ATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCAT CGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGA CTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACC AAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGC GGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACC CACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTA GCGTTTAAACGGGCCCTCTAGACTCGAGCGGCCGCCACCATGAGCCTGTGGAGAAGCA ATGAGGCCACAGTGTACCTGCCCCCTGTGTCTGTGAGCAAGGTGGTGAGCACAGATGA GTATGTGACAAGAACCAACATCTACTACCATGCTGGCAGCAGCAGACTGCTGGCTGTGG GCCACCCCTACTATGCCATCAAGAAGCAAGACAGCAACAAGATTGCTGTGCCCAAGGTG TCTGGCCTGCAGTACAGAGTGTTCAGAGTGAAGCTGCCTGACCCCAACAAGTTTGGCTT CCCTGACACTAGCTTCTATGACCCTGCTAGCCAAAGACTGGTGTGGGCCTGTACTGGGG TGGAGGTAGGTAGAGGGCAGCCACTGGGGGTGGGCATCTCTGGCCACCCCCTGCTGAA CAAGCTGGATGACACAGAGAACAGCAACAAGTATGTGGGCAACTCTGGCACAGACAAC AGAGAGTGCATCAGCATGGACTACAAGCAGACACAGCTGTGCCTGATTGGCTGCAGAC CCCCCATTGGGGAGCACTGGGGCAAAGGCACCCCCTGTAATGCCAACCAAGTCAAGGC TGGAGAGTGCCCCCCCCTGGAGCTGCTGAACACAGTGCTGCAAGATGGGGACATGGTG GACACTGGCTTTGGGGCCATGGACTTCACCACCCTGCAAGCCAACAAGTCTGATGTGCC CCTGGACATCTGCAGCAGCATCTGCAAGTACCCTGACTACCTGAAGATGGTGTCTGAGC CCTATGGGGACATGCTGTTCTTCTACCTGAGAAGAGAGCAGATGTTTGTGAGACACCTG TTCAACAGAGCTGGCACAGTGGGGGAGACAGTGCCTGCTGACCTGTACATCAAGGGCA CCACTGGCACCCTGCCTAGCACAAGCTACTTCCCCACCCCCTCTGGCAGCATGGTGACC TCTGATGCTCAGATCTTCAACAAGCCCTACTGGCTGCAGAGAGCCCAAGGCCACAACAA TGGCATCTGCTGGAGCAATCAGCTGTTTGTGACAGTGGTGGACACCACAAGAAGCACC AACATGTCTGTGTGCTCTGCTGTGAGCAGCTCTGACAGCACCTACAAGAATGACAACTT CAAGGAGTACCTGAGACATGGGGAGGAGTATGACCTGCAGTTCATCTTTCAGCTGTGCA AGATCACCCTGACAGCTGATGTGATGACCTACATCCACAGCATGAACCCTAGCATCCTGG AGGACTGGAACTTTGGCCTGACCCCCCCTCCCTCTGGCACCCTGGAGGACACCTACAGA TATGTGACAAGCCAAGCTGTGACCTGTCAGAAGCCCTCTGCCCCCAAGCCCAAGGATGA CCCCCTGAAGAACTACACCTTCTGGGAGGTGGACCTGAAGGAGAAGTTCTCTGCTGAC CTGGATCAGTTCCCCCTGGGCAGAAAGTTCCTGCTGCAAGCTGGCCTGAAGGCTAGACC CAACTTCAGACTGGGCAAGAGAGCTGCCCCTGCTAGCACAAGCAAGAAGAGCAGCACC AAGAGAAGAAAGGTGAAGAGCTAATAGAAGCTTAAGTTTAAACCGCTGATCAGCCTCG ACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACC CTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGT CTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGG ATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCG GAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAG CGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGC CCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAG CTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCA AAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTC GCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACA ACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTA TTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTG TGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCA TGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAG AAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCC CATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTT TTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGA GGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTC GGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCA CGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGA CAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTT TTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGC TATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAA GCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCA CCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCT TGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTA CTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTC GCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCG TCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTG GATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCT ACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTA CGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTT CTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCAC GAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGG ACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCC AACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAA ATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTAT CATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTC CTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGT GTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGC CCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCG GGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCG CTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGG CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGAC GAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAA CCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG GTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGA AGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGG TAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGCAAGCAGC AGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCT GACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAG GATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATG AGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCT GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGA GGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTC CAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGC AACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTC GCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTC GTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATC CCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTA AGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT GCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATA GTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACA TAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAA GGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTT CAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCC GCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCA ATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTT AGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTC

expression plasmid 35L2-pCDNA3.1 sequence (containing the pre-optimized gene HPV 35L2), SEQ ID NO. 12:

GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATG CCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCG CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTG CTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACAT TGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATAT GGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACC CCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC ATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGT ATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATT ATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCAT CGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGA CTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACC AAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGC GGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACC CACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTA GCGTTTAAACGGGCCCTCTAGACTCGAGCGGCCGCCACCATGCGCCACAAGCGGTCCA CCAAGAGAGTGAAGAGGGCCTCTGCCACACAGCTGTACAGAACCTGCAAGGCAGCAG GAACATGTCCACCTGACGTGATCCCCAAGGTGGAGGGCAACACCGTGGCCGATCAGAT CCTGAAGTACGGCTCTATGGCCGTGTTCTTTGGCGGCCTGGGCATCGGATCTGGAAGCG GAACCGGCGGCCGGAGCGGCTATGTGCCACTGGGAACCACACCACCAACAGCAGCAAC CAATATCCCCATCCGGCCTCCAGTGACAGTGGAGTCCATCCCCCTGGACACCATCGGCCC TCTGGATAGCTCCATCGTGTCTCTGGTGGAGGAGACATCCTTCATCGAGTCTGGAGCACC TGTGGTGACCCCAAGGGTGCCACCTACCACAGGCTTTACAATCACCACATCCACCGACA CCACACCTGCCATCCTGGATGTGACATCCATCTCTACCCACGACAACCCAACCTTCACAG ATCCATCTGTGCTGCACCCACCAACACCAGCAGAGACCAGCGGCCACTTTGTGCTGTCT AGCTCCTCTATCTCCACCCACAACTACGAGGAGATCCCTATGGACACATTCATCGTGTCT ACCGATAGCAACAATATCACAAATAGCACCCCTATCCCAGGCTCCAGACCTACCACAAG GCTGGGCCTGTATAGCAAGGGCACCCAGCAGGTGAAGGTGGTGGACCCAGCCTTTATGA CATCCCCCGCCAAGCTGATCACCTACGATAACCCCGCCTATGAGGGCCTGAATCCAGAC ACCACACTGCAGTTCGAGCACGAGGATATCTCCCTGGCCCCCGACCCTGACTTCATGGA CATCATCGCCCTGCACAGACCAGCCCTGACAAGCAGGAAGGGCACCATCCGCTACTCCC GGGTGGGCAACAAGCGCACAATGCACACCCGGAGCGGCAAGGCAATCGGAGCAAGGG TGCACTACTATCAGGACCTGAGCTCCATCACCGAGGATATCGAGCTGCAGCCACTGCAG CACGTGCCCTCTAGCCTGCCTCACACCACAGTGAGCACATCCCTGAATGACGGCATGTT CGATATCTACGCCCCCATCGACACCGAGGAGGATATCATCTTTTCCGCCTCCTCTAACAAT ACACTGTACACCACATCTAACACCGCCTATGTGCCTAGCAATACCACAATCCCACTGAGC TCCGGCTATGACATCCCTATCACAGCCGGCCCAGATATCGTGTTCAACAGCAATACCATC ACAAATACCGTGCTGCCAGTGCCAACCGGACCAATCTACTCTATCATCGCAGACGGAGG CGACTTCTACCTGCACCCTAGCTACTATCTGCTGAAGCGGAGAAGGAAGCGGATCCCATA TTTCTTTGCCGACGTGAGCGTGGCCGTGTAATAGAAGCTTAAGTTTAAACCGCTGATCAG CCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTT GACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCA TTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGG GAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGA GGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCA TTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCT AGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCG TCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGA CCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGG TTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTG GAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTC GGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGG AATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGC AAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCA GGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACT CCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTA ATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAG TGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATC CATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATG GATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCA CAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCC GGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAG CGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTC ACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGT CATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGC ATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGA GCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCA GGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAG GATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGC TTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGC GTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCG TGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGA GTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGC CATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTT TCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCC CACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATT TCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGT ATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAG CTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGC ATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGC TCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCA ACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACT CGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATA CGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCC CCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGA GCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACAC TAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGCAAG CAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGG GTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA AAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTAT ATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGC GATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATA CGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACC GGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGT CCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGT AGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCA CGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACA TGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGA AGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTG TCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAG AATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGC CACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTC TCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGA TCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAA TGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTT TTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGT ATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGA CGTC

expression plasmid 35L2-op1-pCDNA3.1 sequence (containing optimized gene HPV 35L2-op1), SEQ ID NO. 13:

GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATG CCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCG CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTG CTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACAT TGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATAT GGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACC CCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC ATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGT ATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATT ATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCAT CGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGA CTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACC AAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGC GGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACC CACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTA GCGTTTAAACGGGCCCTCTAGACTCGAGCGGCCGCCACCATGAGACACAAGAGAAGCA CCAAGAGAGTGAAGAGAGCTAGCGCCACACAGCTGTACAGAACCTGCAAGGCCGCCG GCACCTGCCCCCCCGACGTGATCCCCAAGGTGGAGGGCAACACCGTGGCCGATCAGAT CCTGAAGTATGGCAGCATGGCTGTCTTCTTCGGCGGCCTGGGTATCGGCAGCGGAAGCG GCACCGGCGGCAGAAGTGGGTATGTGCCCCTTGGTACGACACCCCCCACCGCCGCCAC CAACATCCCCATCAGACCCCCCGTGACCGTGGAGAGCATCCCCCTGGACACCATCGGCC CCCTGGACAGCAGCATCGTGAGCCTGGTGGAGGAGACTAGCTTCATCGAGAGCGGCGC CCCCGTGGTGACCCCTAGAGTGCCCCCCACCACCGGCTTCACCATCACCACAAGCACGG ACACGACACCCGCCATCCTGGACGTGACCTCCATCAGCACTCACGATAACCCTACCTTTA CCGACCCTAGCGTTCTGCATCCCCCCACGCCCGCCGAGACAAGCGGCCACTTCGTGCTG AGCAGCAGCAGCATCAGCACCCACAACTATGAGGAGATCCCCATGGACACCTTCATCGT GAGCACCGACAGCAACAACATCACAAACTCCACACCCATTCCCGGCAGCAGACCCACC ACAAGACTGGGCCTGTACAGCAAGGGCACACAGCAAGTGAAGGTGGTGGACCCCGCCT TCATGACAAGCCCCGCCAAGCTGATCACCTACGACAACCCCGCCTACGAGGGCCTGAAC CCCGACACAACGCTGCAGTTCGAGCACGAGGACATCAGCCTGGCCCCCGACCCCGACT TCATGGACATCATCGCCCTGCACAGACCCGCCCTGACAAGCAGAAAGGGCACCATCAGA TACAGCAGAGTGGGCAACAAGAGAACCATGCACACAAGAAGCGGCAAGGCCATCGGC GCTAGAGTGCACTACTACCAAGACCTGAGCAGCATCACCGAGGACATCGAGCTGCAGC CCCTGCAGCACGTTCCCTCGTCCCTGCCCCACACCACCGTGAGCACAAGCCTGAACGA CGGCATGTTCGACATCTACGCCCCCATCGACACCGAGGAGGACATCATCTTCAGCGCTA GCAGCAACAACACCCTGTACACCACAAGCAACACCGCCTACGTGCCTAGCAACACCAC CATCCCCCTGAGCAGCGGCTACGACATCCCCATAACTGCCGGCCCCGACATCGTGTTCA ACAGCAACACCATCACCAACACCGTGCTGCCCGTGCCCACCGGCCCCATCTACAGCATC ATCGCCGACGGCGGCGACTTCTACCTGCACCCTAGCTACTACCTGCTGAAGAGAAGAAG AAAGAGAATCCCCTACTTCTTCGCCGACGTGAGCGTGGCCGTGTAATAGAAGCTTAAGT TTAAACCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCC TCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAAT GAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGG GCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTG GGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGC GCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTA CACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGT TCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTG CTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCA TCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGA CTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAG GGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACG CGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAG CAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCC CCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAT AGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCC GCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGA GCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCC CGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCA TGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTC GGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTC AGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAAC TGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGC TGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCG GGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGAT GCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAA ACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATC TGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCG CATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCAT GGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACC GCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGG GCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTC TATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAA GCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTT GGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCA TGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAA GCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTT GTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTG GCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACA ACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTC ACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCT GCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCG CTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCT CACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACAT GTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTT TTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTG CGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGA AGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGC TCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGG TAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGG TGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCA GTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAG CGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATC CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATT TTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGT TTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCA GTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGT CGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACC GCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGG GCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGC CGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCT ACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAA CGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGG TCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGC ACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTAC TCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTC AATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAAC GTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAA CCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGA GCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGT TGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCAT GAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATT TCCCCGAAAAGTGCCACCTGACGTC

expression plasmid 35L2-op2-pCDNA3.1 sequence (containing optimized gene HPV 35L2-op2), SEQ ID NO. 14:

GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATG CCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCG CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTG CTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACAT TGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATAT GGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACC CCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC ATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGT ATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATT ATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCAT CGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGA CTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACC AAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGC GGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACC CACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTA GCGTTTAAACGGGCCCTCTAGACTCGAGCGGCCGCCACCATGAGACACAAGAGAAGCA CCAAGAGAGTGAAGAGAGCCTCTGCCACACAGCTGTACAGAACCTGCAAGGCTGCTGG CACCTGCCCCCCTGATGTGATCCCCAAGGTGGAGGGCAACACAGTGGCTGATCAGATCC TGAAGTATGGCAGCATGGCTGTCTTCTTTGGGGGCCTGGGTATTGGCTCTGGCTCTGGCA CTGGGGGCAGAAGTGGGTATGTGCCCCTTGGTACCACACCCCCCACAGCTGCCACCAAC ATCCCCATCAGACCCCCTGTGACAGTGGAGAGCATCCCCCTGGACACCATTGGCCCCCT GGACAGCAGCATTGTGAGCCTGGTGGAGGAGACTAGCTTCATTGAGTCTGGGGCCCCTG TGGTGACCCCTAGAGTGCCCCCCACCACTGGCTTCACCATCACCACAAGCACAGACACC ACACCTGCCATCCTGGATGTGACCTCCATCAGCACTCATGACAACCCTACCTTTACAGAC CCCTCTGTGCTGCATCCCCCCACCCCTGCTGAGACCTCTGGCCACTTTGTGCTGAGCAG CAGCAGCATCAGCACCCACAACTATGAGGAGATCCCCATGGACACCTTCATTGTGAGCA CAGACAGCAACAACATCACAAACTCCACACCCATTCCTGGCAGCAGACCCACCACAAG ACTGGGCCTGTACAGCAAGGGCACACAGCAAGTGAAGGTGGTGGACCCTGCCTTCATG ACAAGCCCTGCCAAGCTGATCACCTATGACAACCCTGCCTATGAGGGCCTGAACCCTGA CACAACCCTGCAGTTTGAGCATGAGGACATCAGCCTGGCCCCTGACCCTGACTTCATGG ACATCATTGCCCTGCACAGACCTGCCCTGACAAGCAGAAAGGGCACCATCAGATACAGC AGAGTGGGCAACAAGAGAACCATGCACACAAGATCTGGCAAGGCCATTGGGGCTAGAG TGCACTACTACCAAGACCTGAGCAGCATCACAGAGGACATTGAGCTGCAGCCCCTGCA GCATGTGCCTAGCTCCCTGCCCCACACCACAGTGAGCACAAGCCTGAATGATGGCATGT TTGACATCTATGCCCCCATTGACACAGAGGAGGACATCATCTTCTCTGCTAGCAGCAACA ACACCCTGTACACCACAAGCAACACAGCCTATGTGCCTAGCAACACCACCATCCCCCTG AGCTCTGGCTATGACATCCCCATAACTGCTGGCCCTGACATTGTGTTCAACAGCAACACC ATCACCAACACAGTGCTGCCTGTGCCCACTGGCCCCATCTACAGCATCATTGCTGATGGG GGGGACTTCTACCTGCACCCTAGCTACTACCTGCTGAAGAGAAGAAGAAAGAGAATCC CCTACTTCTTTGCTGATGTGTCTGTGGCTGTGTAATAGAAGCTTAAGTTTAAACCGCTGAT CAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTT CCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCAT CGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAG GGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTC TGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGC GCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGC CCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCC CGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTC GACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGAC GGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAAC TGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATT TCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGT GGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTAT GCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAG CAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTA ACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGA CTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGT AGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATA TCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGAT GGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGC ACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGC CCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGC AGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTG TCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCT GTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCT GCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGC GAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCAT CAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCG AGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCC GCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATA GCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCT CGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGAC GAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCT GCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGT TTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCG CCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAA ATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAA TGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCAT AGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAA GCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGC GCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGC CAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGA CTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAA TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCA GCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGC CCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGC AGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTA CACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGC AAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTAC GGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT CAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAA GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTC AGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACG ATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTC ACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGT GGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTA AGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTG TCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTT ACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTC AGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTT ACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTC TGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACC GCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAA ACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAA CTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGC AAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTC CTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGA ATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCAC CTGACGTC

expression plasmid EGFP-pCDNA3.1 sequence (containing fluorescent protein gene EGFP), SEQ ID No. 15:

GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATG CCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCG CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTG CTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACAT TGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATAT GGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACC CCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC ATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGT ATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATT ATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCAT CGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGA CTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACC AAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGC GGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACC CACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTA GCGTTTAAACGGGCCCTCTAGACTCGAGCGGCCGCCACCATGGTGAGCAAGGGCGAGG AGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCA CAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTG AAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCT GACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCT TCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGAC GGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCA TCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGA GTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCA AGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCA CTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACC TGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCT GCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAT AGAAGCTTAAGTTTAAACCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCT GTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTT TCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGG GGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCT GGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGG GGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGC AGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCC TTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGG TTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCA CGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTC TTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCT TTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAAC AAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCC CAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGG TGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTA GTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTC CGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCC TCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGC AAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGG ATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGG AGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTG TTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGC CCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTT CCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGG CGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCA TCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACC ACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGAT CAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGC TCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTG CCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGT GTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGG CGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCG CATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATG ACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTA TGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCG GGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTA CAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAG TTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCT AGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAT TCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGA GCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGT GCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGC TCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTA TCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAA GAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTC CCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCC TTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGT CGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTG AAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTG AAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG CTGGTAGCGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAA GAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTA AGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAA ATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGC TTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGAC TCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAA TGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCC GGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAAT TGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGC CATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGG TTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCT CCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTA TGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGG TGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCC GGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGG AAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGA TGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTG GGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGA AATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGT CTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGC ACATTTCCCCGAAAAGTGCCACCTGACGTC

step 2: 293FT cells were plated at 8.0X 10⁶Density of cells/dish in 10cm Petri dish, 12ml/dish complete Medium, 37 ℃, 5% CO₂And (5) culturing in an incubator, and paving for 16-24 hours.

And step 3: and after the cell density is 80-90%, performing transfection. 1h before transfection, cell culture medium was aspirated and 12ml of 2% FBS transfection medium was added.

And 4, step 4: dissolving the structural gene expression plasmid and the fluorescent protein report plasmid in a certain mass ratio (the mass ratio of the structural gene expression plasmids 35L1 and 35L2 to the fluorescent protein report plasmid EGFP is 1:0.3:0.5) in 1.0ml of a culture medium without resistance and bovine serum to obtain a plasmid mixture;

and 5: adding PEI, mixing, incubating at room temperature for 15min to form PEI-DNA complex, adding into a plate, placing at 37 deg.C and 5% CO₂Culturing in an incubator.

Step 6: after 6h, the culture medium is aspirated, 15ml to 20ml of fresh DMEM complete medium is added, the temperature is 37 ℃, and 5% CO is added₂Culturing for 48-96 h in an incubator.

And 7: the cell culture was decanted, 3ml PBS was added to the dish, the cells were collected and centrifuged at 1500rpm for 10min, and the supernatant was discarded.

And 8: the cell pellet was washed again with 5ml PBS at 1500rpm/min, centrifuged for 10min, and the supernatant was discarded.

And step 9: adding a lysis solution which is equal in volume to the cell sediment and contains nuclease into the cell sediment, uniformly mixing once within 3-4 h, and cracking for 16-24 h at 37 ℃.

Step 10: taking out the lysate, carrying out ice bath for 10min, centrifuging for 10min at 4 ℃ and 6000g/min, collecting supernatant, namely the prepared pseudovirus, and storing at-65 ℃ to-85 ℃.

Example 3 detection of HPV type 35 pseudovirus Using enzyme-linked fluorescent Spot Analyzer

Step 1: 293FT cells in logarithmic growth phase were collected, counted on a hemocytometer and diluted to a density of 1.5X 10 with DMEM complete medium⁵One per ml.

Step 2: in a 96-well plate, 150. mu.l of 293FT cells per well were added and the mixture was incubated at 37 ℃ with 5% CO₂The culture is carried out for 16-24 h in the incubator.

And step 3: taking pseudovirus solution, diluting by 200 times or proper times by using a DMEM complete culture medium, then diluting according to a 2-time-by-time ratio, and continuously diluting for 4-8 dilutions.

And 4, step 4: adding 50 mul of pseudovirus diluent into each well, setting 8 wells for each dilution, setting a blank control which is 50 mul of DMEM complete culture medium, and detecting the number of green fluorescent dots in each well by using an enzyme-linked fluorescent spot analyzer after culturing for 72 hours.

And 5: taking the logarithm value (taking 10 as the base number) of the dilution factor as an X axis, taking the average value of the fluorescence points as a Y axis, performing linear fitting, and calculating the dilution factor when the fluorescence points are 500 fluorescence points according to a linear fitting curve, namely the dilution factor used by the pseudovirus, wherein the results are shown in tables 1-3 and figures 1-3.

TABLE 1 summary of the results before optimization of HPV35 type pseudovirus plasmid gene sequences

TABLE 2 summary of op1 results after optimization of HPV type 35 pseudovirus plasmid gene sequences

TABLE 3 summary of op2 results after optimization of HPV type 35 pseudovirus plasmid gene sequences

The results show that: before the gene sequence of the HPV35 type pseudovirus plasmid is optimized, the number of fluorescent points required for detecting the titer of HPV35 type neutralizing antibodies cannot be reached even if the HPV35 type pseudovirus is multiplied by a prime number, the HPV35 type pseudovirus prepared by adopting the HPV 35L1-op1+ HPV 35L2-op1 expression plasmid is diluted by 839 times, and although the neutralization titer detection can be carried out, the usage amount of the pseudovirus is large, and the detection cost is high. The dilution factor for preparing the HPV35 type pseudovirus by adopting the HPV 35L1-op2+ HPV 35L2-op2 expression plasmid is 4046 times, and in conclusion, the HPV35 type pseudovirus plasmid cannot be used for detecting the titer of the neutralizing antibody before the gene sequence is optimized. After the HPV 35L1-op2+ HPV 35L2-op2 expression plasmid is adopted to prepare the HPV35 type pseudovirion, the good linear relation is formed between the mean value of the fluorescence points and the logarithm value of the dilution multiple, and the dilution multiple of the pseudovirion is 4046, so that the detection of the titer of the HPV35 type neutralizing antibody is free from cytotoxicity, the detection accuracy of the titer of the neutralizing antibody is good, and more samples for detecting the titer of the neutralizing antibody can be prepared by preparing a batch of pseudovirion, so that the detection cost is greatly reduced.

Example 4 detection of neutralizing antibody Titers of HPV type 35 sera Using pseudoviruses

Step 1: 293FT cells at 1.5X 10⁴The density of/well was pre-plated in 96 well cell culture plates in 100. mu.l per well, 37 ℃ with 5% CO₂Culturing in an incubator until the cells adhere to the wall.

Step 2: and (3) diluting the serum sample to 320 times by using DMEM complete culture medium respectively, and carrying out serial multiple ratio dilution by proper dilution times, wherein the serum sample is a double-hole.

And step 3: and (3) taking the pseudovirus solution, diluting the pseudovirus solution by using a DMEM complete culture medium until the number of fluorescence spots in each well is 200-1000, uniformly mixing, and simultaneously setting 8 positive control (pseudovirus control) wells and 8 blank control (culture medium control) wells.

And 4, step 4: adding 60 mul pseudovirus diluent into 60 mul serum diluent, shaking and mixing uniformly for 1 minute, and then placing in a constant temperature incubator at 25 +/-2 ℃ for incubation for 60 +/-2 minutes.

And 5: accurately aspirate 100. mu.l of serum-pseudovirus mix and slowly and carefully add to the 96-well pre-plated cell plate.

Step 6: at 37 ℃ with 5% CO₂And (5) after culturing for 68-76 hours in an incubator, observing the result by using an enzyme-linked fluorescent spot analyzer.

And 7: the Cut-off value is calculated according to the following formula: cut-off value-mean value of fluorescent spot number of pseudovirus control ÷ 2

And 8: the maximum dilution multiple corresponding to the average fluorescence point number of the serum sample being less than or equal to the Cut-off value is the neutralizing antibody titer of the sample.

Taking 4HPV 35 serum samples, and performing the steps, wherein the Cut-off value is 328, and the neutralizing antibody titers of the samples 1-4 are 1280, 640, 320 and 1280 respectively. The results are shown in Table 4 and FIG. 4.

TABLE 4 summary of the results of HPV type 35 pseudovirus neutralization antibody titer test

The results show that: when the pseudovirus prepared after the HPV35 pseudovirus plasmid gene sequence is diluted by 839 times and used for neutralizing antibody titer detection, the average value of the number of the pseudovirus control fluorescence points is 656, the number of the pseudovirus control fluorescence points is 200-1000, and the method is suitable for neutralizing antibody titer detection of a serum sample. The method has the advantages of low detection cost, good detection result accuracy, good repeatability and the like.

Sequence listing

<110> Shanghai Bowei Biotechnology Ltd

<120> human papilloma virus 35/HPV 35 type L1, and preparation and application thereof

<160> 15

<170> SIPOSequenceListing 1.0

<210> 1

<211> 502

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<400> 1

Met Ser Leu Trp Arg Ser Asn Glu Ala Thr Val Tyr Leu Pro Pro Val

1 5 10 15

Ser Val Ser Lys Val Val Ser Thr Asp Glu Tyr Val Thr Arg Thr Asn

20 25 30

Ile Tyr Tyr His Ala Gly Ser Ser Arg Leu Leu Ala Val Gly His Pro

35 40 45

Tyr Tyr Ala Ile Lys Lys Gln Asp Ser Asn Lys Ile Ala Val Pro Lys

50 55 60

Val Ser Gly Leu Gln Tyr Arg Val Phe Arg Val Lys Leu Pro Asp Pro

65 70 75 80

Asn Lys Phe Gly Phe Pro Asp Thr Ser Phe Tyr Asp Pro Ala Ser Gln

85 90 95

Arg Leu Val Trp Ala Cys Thr Gly Val Glu Val Gly Arg Gly Gln Pro

100 105 110

Leu Gly Val Gly Ile Ser Gly His Pro Leu Leu Asn Lys Leu Asp Asp

115 120 125

Thr Glu Asn Ser Asn Lys Tyr Val Gly Asn Ser Gly Thr Asp Asn Arg

130 135 140

Glu Cys Ile Ser Met Asp Tyr Lys Gln Thr Gln Leu Cys Leu Ile Gly

145 150 155 160

Cys Arg Pro Pro Ile Gly Glu His Trp Gly Lys Gly Thr Pro Cys Asn

165 170 175

Ala Asn Gln Val Lys Ala Gly Glu Cys Pro Pro Leu Glu Leu Leu Asn

180 185 190

Thr Val Leu Gln Asp Gly Asp Met Val Asp Thr Gly Phe Gly Ala Met

195 200 205

Asp Phe Thr Thr Leu Gln Ala Asn Lys Ser Asp Val Pro Leu Asp Ile

210 215 220

Cys Ser Ser Ile Cys Lys Tyr Pro Asp Tyr Leu Lys Met Val Ser Glu

225 230 235 240

Pro Tyr Gly Asp Met Leu Phe Phe Tyr Leu Arg Arg Glu Gln Met Phe

245 250 255

Val Arg His Leu Phe Asn Arg Ala Gly Thr Val Gly Glu Thr Val Pro

260 265 270

Ala Asp Leu Tyr Ile Lys Gly Thr Thr Gly Thr Leu Pro Ser Thr Ser

275 280 285

Tyr Phe Pro Thr Pro Ser Gly Ser Met Val Thr Ser Asp Ala Gln Ile

290 295 300

Phe Asn Lys Pro Tyr Trp Leu Gln Arg Ala Gln Gly His Asn Asn Gly

305 310 315 320

Ile Cys Trp Ser Asn Gln Leu Phe Val Thr Val Val Asp Thr Thr Arg

325 330 335

Ser Thr Asn Met Ser Val Cys Ser Ala Val Ser Ser Ser Asp Ser Thr

340 345 350

Tyr Lys Asn Asp Asn Phe Lys Glu Tyr Leu Arg His Gly Glu Glu Tyr

355 360 365

Asp Leu Gln Phe Ile Phe Gln Leu Cys Lys Ile Thr Leu Thr Ala Asp

370 375 380

Val Met Thr Tyr Ile His Ser Met Asn Pro Ser Ile Leu Glu Asp Trp

385 390 395 400

Asn Phe Gly Leu Thr Pro Pro Pro Ser Gly Thr Leu Glu Asp Thr Tyr

405 410 415

Arg Tyr Val Thr Ser Gln Ala Val Thr Cys Gln Lys Pro Ser Ala Pro

420 425 430

Lys Pro Lys Asp Asp Pro Leu Lys Asn Tyr Thr Phe Trp Glu Val Asp

435 440 445

Leu Lys Glu Lys Phe Ser Ala Asp Leu Asp Gln Phe Pro Leu Gly Arg

450 455 460

Lys Phe Leu Leu Gln Ala Gly Leu Lys Ala Arg Pro Asn Phe Arg Leu

465 470 475 480

Gly Lys Arg Ala Ala Pro Ala Ser Thr Ser Lys Lys Ser Ser Thr Lys

485 490 495

Arg Arg Lys Val Lys Ser

500

<210> 2

<211> 469

<212> PRT

<213> Artificial Sequence (Artificial Sequence)

<400> 2

Met Arg His Lys Arg Ser Thr Lys Arg Val Lys Arg Ala Ser Ala Thr

1 5 10 15

Gln Leu Tyr Arg Thr Cys Lys Ala Ala Gly Thr Cys Pro Pro Asp Val

20 25 30

Ile Pro Lys Val Glu Gly Asn Thr Val Ala Asp Gln Ile Leu Lys Tyr

35 40 45

Gly Ser Met Ala Val Phe Phe Gly Gly Leu Gly Ile Gly Ser Gly Ser

50 55 60

Gly Thr Gly Gly Arg Ser Gly Tyr Val Pro Leu Gly Thr Thr Pro Pro

65 70 75 80

Thr Ala Ala Thr Asn Ile Pro Ile Arg Pro Pro Val Thr Val Glu Ser

85 90 95

Ile Pro Leu Asp Thr Ile Gly Pro Leu Asp Ser Ser Ile Val Ser Leu

100 105 110

Val Glu Glu Thr Ser Phe Ile Glu Ser Gly Ala Pro Val Val Thr Pro

115 120 125

Arg Val Pro Pro Thr Thr Gly Phe Thr Ile Thr Thr Ser Thr Asp Thr

130 135 140

Thr Pro Ala Ile Leu Asp Val Thr Ser Ile Ser Thr His Asp Asn Pro

145 150 155 160

Thr Phe Thr Asp Pro Ser Val Leu His Pro Pro Thr Pro Ala Glu Thr

165 170 175

Ser Gly His Phe Val Leu Ser Ser Ser Ser Ile Ser Thr His Asn Tyr

180 185 190

Glu Glu Ile Pro Met Asp Thr Phe Ile Val Ser Thr Asp Ser Asn Asn

195 200 205

Ile Thr Asn Ser Thr Pro Ile Pro Gly Ser Arg Pro Thr Thr Arg Leu

210 215 220

Gly Leu Tyr Ser Lys Gly Thr Gln Gln Val Lys Val Val Asp Pro Ala

225 230 235 240

Phe Met Thr Ser Pro Ala Lys Leu Ile Thr Tyr Asp Asn Pro Ala Tyr

245 250 255

Glu Gly Leu Asn Pro Asp Thr Thr Leu Gln Phe Glu His Glu Asp Ile

260 265 270

Ser Leu Ala Pro Asp Pro Asp Phe Met Asp Ile Ile Ala Leu His Arg

275 280 285

Pro Ala Leu Thr Ser Arg Lys Gly Thr Ile Arg Tyr Ser Arg Val Gly

290 295 300

Asn Lys Arg Thr Met His Thr Arg Ser Gly Lys Ala Ile Gly Ala Arg

305 310 315 320

Val His Tyr Tyr Gln Asp Leu Ser Ser Ile Thr Glu Asp Ile Glu Leu

325 330 335

Gln Pro Leu Gln His Val Pro Ser Ser Leu Pro His Thr Thr Val Ser

340 345 350

Thr Ser Leu Asn Asp Gly Met Phe Asp Ile Tyr Ala Pro Ile Asp Thr

355 360 365

Glu Glu Asp Ile Ile Phe Ser Ala Ser Ser Asn Asn Thr Leu Tyr Thr

370 375 380

Thr Ser Asn Thr Ala Tyr Val Pro Ser Asn Thr Thr Ile Pro Leu Ser

385 390 395 400

Ser Gly Tyr Asp Ile Pro Ile Thr Ala Gly Pro Asp Ile Val Phe Asn

405 410 415

Ser Asn Thr Ile Thr Asn Thr Val Leu Pro Val Pro Thr Gly Pro Ile

420 425 430

Tyr Ser Ile Ile Ala Asp Gly Gly Asp Phe Tyr Leu His Pro Ser Tyr

435 440 445

Tyr Leu Leu Lys Arg Arg Arg Lys Arg Ile Pro Tyr Phe Phe Ala Asp

450 455 460

Val Ser Val Ala Val

465

<210> 3

<211> 1506

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 3

atgtctctgt ggagatccaa cgaggccact gtctacctgc ctccagtttc ggtgtctaag 60

gttgtgtcca cggacgagta cgtcactaga accaacatct actaccacgc aggttcctct 120

agactcctgg ctgttggtca cccttactat gccattaaga agcaggactc gaacaagatc 180

gccgtcccaa aggtttctgg cttgcagtac agagtgttca gagttaagct gccagaccct 240

aacaagttcg gatttccaga cacctccttc tacgaccctg cttcccagag attggtttgg 300

gcatgcactg gagtcgaggt gggcagaggt cagccattgg gagttggtat ctctggccac 360

cctttgctga acaagctcga cgataccgag aactccaaca agtacgttgg caactctgga 420

accgacaaca gagagtgcat ctcgatggac tacaagcaga cccagttgtg tctcatcgga 480

tgcagaccac ctattggtga acattgggga aagggcaccc cttgcaacgc caaccaggtc 540

aaggccggag agtgtcctcc attggagctt ctgaacactg ttctccaaga tggtgacatg 600

gttgacaccg gctttggtgc tatggacttc acgaccttgc aggccaacaa gtccgacgtg 660

ccacttgaca tctgttcttc catttgcaag taccctgatt acctgaagat ggtttcggag 720

ccatacggag acatgctctt cttttacctg agaagagagc agatgttcgt gagacacttg 780

ttcaacagag caggaactgt tggtgaaacg gtccctgctg acctgtacat caagggcacc 840

actggtacgt tgccatctac ctcgtacttc cctactccat ctggttcgat ggtcacctcc 900

gatgcccaga tcttcaacaa gccatactgg ttgcagagag cccagggaca caacaatggc 960

atttgctggt ccaaccagct gttcgtgacc gtcgttgaca ctacgagatc caccaacatg 1020

tcggtgtgtt ctgcagtcag ctcttccgac tcgacctaca agaacgacaa cttcaaggag 1080

tacctcagac acggtgaaga gtacgacctg caattcatct tccagttgtg caagatcacc 1140

ctgactgctg acgttatgac gtacattcac tccatgaacc cttcgatcct ggaggactgg 1200

aacttcggtc ttactccacc tccatctggc accttggagg acacttacag atatgtcacc 1260

tcccaagctg ttacgtgtca gaagccttcg gccccaaagc ctaaggacga tccactgaag 1320

aactacacct tctgggaggt tgacctgaag gagaagttct ccgcagacct cgaccagttc 1380

ccattgggca gaaagttcct gctccaagct ggattgaagg ccagacctaa cttcagactt 1440

ggcaagagag ccgctccagc atctacctct aagaaatcgt ccacgaagcg cagaaaggtg 1500

aagtcg 1506

<210> 4

<211> 1506

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 4

atgagcctgt ggagaagcaa cgaggccacc gtgtacctgc ccccggtgag cgtgagcaag 60

gtggtgagca ccgacgagta cgtgacaaga accaacatct actaccacgc cggcagcagc 120

agactgctgg ccgtgggcca cccctactac gccatcaaga agcaagacag caacaagatc 180

gccgtgccca aggtgagcgg cctgcagtac agagtgttca gagtgaagct gcccgacccc 240

aacaagttcg gcttccccga cactagcttc tatgacccgg ctagccaaag actggtgtgg 300

gcgtgtacgg gcgtggaggt aggtagaggg cagccactgg gcgtgggcat cagcggccac 360

cccctgctga acaagctgga cgacaccgag aacagcaaca agtacgtggg caacagcggc 420

accgacaaca gagagtgcat cagcatggac tacaagcaga cacagctgtg cctgatcggc 480

tgcagacccc ccatcggcga gcactggggc aaaggcaccc cgtgtaacgc taaccaagtc 540

aaggcgggag agtgcccccc cctggagctg ctgaacaccg tgctgcaaga cggcgacatg 600

gtggacaccg gcttcggcgc catggacttc accaccctgc aagccaacaa gagcgacgtg 660

cccctggaca tctgcagcag catctgcaag taccccgact acctgaagat ggtgagcgag 720

ccctacggcg acatgctgtt cttctacctg agaagagagc agatgttcgt gagacacctg 780

ttcaacagag ccggcaccgt gggcgagacc gtgcccgccg acctgtacat caagggcacc 840

accggcaccc tgcctagcac aagctacttc cccacgccta gcggcagcat ggtgacaagc 900

gacgctcaga tcttcaacaa gccctactgg ctgcagagag cccaaggcca caacaacggc 960

atctgctgga gcaatcagct gttcgtgacc gtggtggaca ccacaagaag caccaacatg 1020

agcgtgtgca gcgccgtgag cagcagcgac agcacctaca agaacgacaa cttcaaggag 1080

tacctgagac acggcgagga gtacgacctg cagttcatct ttcagctgtg caagatcacc 1140

ctgaccgccg acgtgatgac ctacatccac agcatgaacc ctagcatcct ggaggactgg 1200

aacttcggcc tgaccccccc tcctagcggc accctggagg acacctacag atacgtgaca 1260

agccaagccg tgacctgtca gaagcctagc gcccccaagc ccaaggacga ccccctgaag 1320

aactacacct tctgggaggt ggacctgaag gagaagttca gcgccgacct ggatcagttc 1380

cccctgggca gaaagttcct gctgcaagcc ggcctgaagg ctagacccaa cttcagactg 1440

ggcaagagag ccgcccccgc tagcacaagc aagaagagca gcaccaagag aagaaaggtg 1500

aagagc 1506

<210> 5

<211> 1506

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 5

atgagcctgt ggagaagcaa tgaggccaca gtgtacctgc cccctgtgtc tgtgagcaag 60

gtggtgagca cagatgagta tgtgacaaga accaacatct actaccatgc tggcagcagc 120

agactgctgg ctgtgggcca cccctactat gccatcaaga agcaagacag caacaagatt 180

gctgtgccca aggtgtctgg cctgcagtac agagtgttca gagtgaagct gcctgacccc 240

aacaagtttg gcttccctga cactagcttc tatgaccctg ctagccaaag actggtgtgg 300

gcctgtactg gggtggaggt aggtagaggg cagccactgg gggtgggcat ctctggccac 360

cccctgctga acaagctgga tgacacagag aacagcaaca agtatgtggg caactctggc 420

acagacaaca gagagtgcat cagcatggac tacaagcaga cacagctgtg cctgattggc 480

tgcagacccc ccattgggga gcactggggc aaaggcaccc cctgtaatgc caaccaagtc 540

aaggctggag agtgcccccc cctggagctg ctgaacacag tgctgcaaga tggggacatg 600

gtggacactg gctttggggc catggacttc accaccctgc aagccaacaa gtctgatgtg 660

cccctggaca tctgcagcag catctgcaag taccctgact acctgaagat ggtgtctgag 720

ccctatgggg acatgctgtt cttctacctg agaagagagc agatgtttgt gagacacctg 780

ttcaacagag ctggcacagt gggggagaca gtgcctgctg acctgtacat caagggcacc 840

actggcaccc tgcctagcac aagctacttc cccaccccct ctggcagcat ggtgacctct 900

gatgctcaga tcttcaacaa gccctactgg ctgcagagag cccaaggcca caacaatggc 960

atctgctgga gcaatcagct gtttgtgaca gtggtggaca ccacaagaag caccaacatg 1020

tctgtgtgct ctgctgtgag cagctctgac agcacctaca agaatgacaa cttcaaggag 1080

tacctgagac atggggagga gtatgacctg cagttcatct ttcagctgtg caagatcacc 1140

ctgacagctg atgtgatgac ctacatccac agcatgaacc ctagcatcct ggaggactgg 1200

aactttggcc tgaccccccc tccctctggc accctggagg acacctacag atatgtgaca 1260

agccaagctg tgacctgtca gaagccctct gcccccaagc ccaaggatga ccccctgaag 1320

aactacacct tctgggaggt ggacctgaag gagaagttct ctgctgacct ggatcagttc 1380

cccctgggca gaaagttcct gctgcaagct ggcctgaagg ctagacccaa cttcagactg 1440

ggcaagagag ctgcccctgc tagcacaagc aagaagagca gcaccaagag aagaaaggtg 1500

aagagc 1506

<210> 6

<211> 1407

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 6

atgcgccaca agcggtccac caagagagtg aagagggcct ctgccacaca gctgtacaga 60

acctgcaagg cagcaggaac atgtccacct gacgtgatcc ccaaggtgga gggcaacacc 120

gtggccgatc agatcctgaa gtacggctct atggccgtgt tctttggcgg cctgggcatc 180

ggatctggaa gcggaaccgg cggccggagc ggctatgtgc cactgggaac cacaccacca 240

acagcagcaa ccaatatccc catccggcct ccagtgacag tggagtccat ccccctggac 300

accatcggcc ctctggatag ctccatcgtg tctctggtgg aggagacatc cttcatcgag 360

tctggagcac ctgtggtgac cccaagggtg ccacctacca caggctttac aatcaccaca 420

tccaccgaca ccacacctgc catcctggat gtgacatcca tctctaccca cgacaaccca 480

accttcacag atccatctgt gctgcaccca ccaacaccag cagagaccag cggccacttt 540

gtgctgtcta gctcctctat ctccacccac aactacgagg agatccctat ggacacattc 600

atcgtgtcta ccgatagcaa caatatcaca aatagcaccc ctatcccagg ctccagacct 660

accacaaggc tgggcctgta tagcaagggc acccagcagg tgaaggtggt ggacccagcc 720

tttatgacat cccccgccaa gctgatcacc tacgataacc ccgcctatga gggcctgaat 780

ccagacacca cactgcagtt cgagcacgag gatatctccc tggcccccga ccctgacttc 840

atggacatca tcgccctgca cagaccagcc ctgacaagca ggaagggcac catccgctac 900

tcccgggtgg gcaacaagcg cacaatgcac acccggagcg gcaaggcaat cggagcaagg 960

gtgcactact atcaggacct gagctccatc accgaggata tcgagctgca gccactgcag 1020

cacgtgccct ctagcctgcc tcacaccaca gtgagcacat ccctgaatga cggcatgttc 1080

gatatctacg cccccatcga caccgaggag gatatcatct tttccgcctc ctctaacaat 1140

acactgtaca ccacatctaa caccgcctat gtgcctagca ataccacaat cccactgagc 1200

tccggctatg acatccctat cacagccggc ccagatatcg tgttcaacag caataccatc 1260

acaaataccg tgctgccagt gccaaccgga ccaatctact ctatcatcgc agacggaggc 1320

gacttctacc tgcaccctag ctactatctg ctgaagcgga gaaggaagcg gatcccatat 1380

ttctttgccg acgtgagcgt ggccgtg 1407

<210> 7

<211> 1407

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 7

atgagacaca agagaagcac caagagagtg aagagagcta gcgccacaca gctgtacaga 60

acctgcaagg ccgccggcac ctgccccccc gacgtgatcc ccaaggtgga gggcaacacc 120

gtggccgatc agatcctgaa gtatggcagc atggctgtct tcttcggcgg cctgggtatc 180

ggcagcggaa gcggcaccgg cggcagaagt gggtatgtgc cccttggtac gacacccccc 240

accgccgcca ccaacatccc catcagaccc cccgtgaccg tggagagcat ccccctggac 300

accatcggcc ccctggacag cagcatcgtg agcctggtgg aggagactag cttcatcgag 360

agcggcgccc ccgtggtgac ccctagagtg ccccccacca ccggcttcac catcaccaca 420

agcacggaca cgacacccgc catcctggac gtgacctcca tcagcactca cgataaccct 480

acctttaccg accctagcgt tctgcatccc cccacgcccg ccgagacaag cggccacttc 540

gtgctgagca gcagcagcat cagcacccac aactatgagg agatccccat ggacaccttc 600

atcgtgagca ccgacagcaa caacatcaca aactccacac ccattcccgg cagcagaccc 660

accacaagac tgggcctgta cagcaagggc acacagcaag tgaaggtggt ggaccccgcc 720

ttcatgacaa gccccgccaa gctgatcacc tacgacaacc ccgcctacga gggcctgaac 780

cccgacacaa cgctgcagtt cgagcacgag gacatcagcc tggcccccga ccccgacttc 840

atggacatca tcgccctgca cagacccgcc ctgacaagca gaaagggcac catcagatac 900

agcagagtgg gcaacaagag aaccatgcac acaagaagcg gcaaggccat cggcgctaga 960

gtgcactact accaagacct gagcagcatc accgaggaca tcgagctgca gcccctgcag 1020

cacgttccct cgtccctgcc ccacaccacc gtgagcacaa gcctgaacga cggcatgttc 1080

gacatctacg cccccatcga caccgaggag gacatcatct tcagcgctag cagcaacaac 1140

accctgtaca ccacaagcaa caccgcctac gtgcctagca acaccaccat ccccctgagc 1200

agcggctacg acatccccat aactgccggc cccgacatcg tgttcaacag caacaccatc 1260

accaacaccg tgctgcccgt gcccaccggc cccatctaca gcatcatcgc cgacggcggc 1320

gacttctacc tgcaccctag ctactacctg ctgaagagaa gaagaaagag aatcccctac 1380

ttcttcgccg acgtgagcgt ggccgtg 1407

<210> 8

<211> 1407

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 8

atgagacaca agagaagcac caagagagtg aagagagcct ctgccacaca gctgtacaga 60

acctgcaagg ctgctggcac ctgcccccct gatgtgatcc ccaaggtgga gggcaacaca 120

gtggctgatc agatcctgaa gtatggcagc atggctgtct tctttggggg cctgggtatt 180

ggctctggct ctggcactgg gggcagaagt gggtatgtgc cccttggtac cacacccccc 240

acagctgcca ccaacatccc catcagaccc cctgtgacag tggagagcat ccccctggac 300

accattggcc ccctggacag cagcattgtg agcctggtgg aggagactag cttcattgag 360

tctggggccc ctgtggtgac ccctagagtg ccccccacca ctggcttcac catcaccaca 420

agcacagaca ccacacctgc catcctggat gtgacctcca tcagcactca tgacaaccct 480

acctttacag acccctctgt gctgcatccc cccacccctg ctgagacctc tggccacttt 540

gtgctgagca gcagcagcat cagcacccac aactatgagg agatccccat ggacaccttc 600

attgtgagca cagacagcaa caacatcaca aactccacac ccattcctgg cagcagaccc 660

accacaagac tgggcctgta cagcaagggc acacagcaag tgaaggtggt ggaccctgcc 720

ttcatgacaa gccctgccaa gctgatcacc tatgacaacc ctgcctatga gggcctgaac 780

cctgacacaa ccctgcagtt tgagcatgag gacatcagcc tggcccctga ccctgacttc 840

atggacatca ttgccctgca cagacctgcc ctgacaagca gaaagggcac catcagatac 900

agcagagtgg gcaacaagag aaccatgcac acaagatctg gcaaggccat tggggctaga 960

gtgcactact accaagacct gagcagcatc acagaggaca ttgagctgca gcccctgcag 1020

catgtgccta gctccctgcc ccacaccaca gtgagcacaa gcctgaatga tggcatgttt 1080

gacatctatg cccccattga cacagaggag gacatcatct tctctgctag cagcaacaac 1140

accctgtaca ccacaagcaa cacagcctat gtgcctagca acaccaccat ccccctgagc 1200

tctggctatg acatccccat aactgctggc cctgacattg tgttcaacag caacaccatc 1260

accaacacag tgctgcctgt gcccactggc cccatctaca gcatcattgc tgatgggggg 1320

gacttctacc tgcaccctag ctactacctg ctgaagagaa gaagaaagag aatcccctac 1380

ttctttgctg atgtgtctgt ggctgtg 1407

<210> 9

<211> 6882

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 9

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

gtttaaacgg gccctctaga ctcgagcggc cgccaccatg tctctgtgga gatccaacga 960

ggccactgtc tacctgcctc cagtttcggt gtctaaggtt gtgtccacgg acgagtacgt 1020

cactagaacc aacatctact accacgcagg ttcctctaga ctcctggctg ttggtcaccc 1080

ttactatgcc attaagaagc aggactcgaa caagatcgcc gtcccaaagg tttctggctt 1140

gcagtacaga gtgttcagag ttaagctgcc agaccctaac aagttcggat ttccagacac 1200

ctccttctac gaccctgctt cccagagatt ggtttgggca tgcactggag tcgaggtggg 1260

cagaggtcag ccattgggag ttggtatctc tggccaccct ttgctgaaca agctcgacga 1320

taccgagaac tccaacaagt acgttggcaa ctctggaacc gacaacagag agtgcatctc 1380

gatggactac aagcagaccc agttgtgtct catcggatgc agaccaccta ttggtgaaca 1440

ttggggaaag ggcacccctt gcaacgccaa ccaggtcaag gccggagagt gtcctccatt 1500

ggagcttctg aacactgttc tccaagatgg tgacatggtt gacaccggct ttggtgctat 1560

ggacttcacg accttgcagg ccaacaagtc cgacgtgcca cttgacatct gttcttccat 1620

ttgcaagtac cctgattacc tgaagatggt ttcggagcca tacggagaca tgctcttctt 1680

ttacctgaga agagagcaga tgttcgtgag acacttgttc aacagagcag gaactgttgg 1740

tgaaacggtc cctgctgacc tgtacatcaa gggcaccact ggtacgttgc catctacctc 1800

gtacttccct actccatctg gttcgatggt cacctccgat gcccagatct tcaacaagcc 1860

atactggttg cagagagccc agggacacaa caatggcatt tgctggtcca accagctgtt 1920

cgtgaccgtc gttgacacta cgagatccac caacatgtcg gtgtgttctg cagtcagctc 1980

ttccgactcg acctacaaga acgacaactt caaggagtac ctcagacacg gtgaagagta 2040

cgacctgcaa ttcatcttcc agttgtgcaa gatcaccctg actgctgacg ttatgacgta 2100

cattcactcc atgaaccctt cgatcctgga ggactggaac ttcggtctta ctccacctcc 2160

atctggcacc ttggaggaca cttacagata tgtcacctcc caagctgtta cgtgtcagaa 2220

gccttcggcc ccaaagccta aggacgatcc actgaagaac tacaccttct gggaggttga 2280

cctgaaggag aagttctccg cagacctcga ccagttccca ttgggcagaa agttcctgct 2340

ccaagctgga ttgaaggcca gacctaactt cagacttggc aagagagccg ctccagcatc 2400

tacctctaag aaatcgtcca cgaagcgcag aaaggtgaag tcgtaataga agcttaagtt 2460

taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 2520

cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 2580

aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 2640

aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 2700

ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 2760

gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 2820

ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 2880

gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 2940

ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 3000

gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 3060

tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 3120

tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 3180

aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 3240

aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 3300

cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 3360

cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 3420

ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 3480

gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 3540

tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 3600

agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 3660

ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 3720

cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 3780

agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 3840

cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 3900

atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 3960

tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 4020

acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 4080

gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 4140

cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 4200

tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 4260

tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 4320

cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 4380

ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 4440

gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 4500

gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 4560

ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 4620

aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 4680

catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 4740

cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 4800

tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 4860

cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 4920

gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 4980

tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 5040

acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 5100

aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 5160

cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 5220

gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 5280

tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 5340

tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 5400

cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 5460

gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 5520

ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 5580

ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 5640

ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga 5700

aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 5760

gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 5820

cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 5880

gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 5940

tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 6000

ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 6060

ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 6120

atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 6180

cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 6240

tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 6300

aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 6360

tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 6420

ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 6480

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 6540

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 6600

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 6660

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 6720

gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 6780

cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 6840

ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc 6882

<210> 10

<211> 6882

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 10

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

gtttaaacgg gccctctaga ctcgagcggc cgccaccatg agcctgtgga gaagcaacga 960

ggccaccgtg tacctgcccc cggtgagcgt gagcaaggtg gtgagcaccg acgagtacgt 1020

gacaagaacc aacatctact accacgccgg cagcagcaga ctgctggccg tgggccaccc 1080

ctactacgcc atcaagaagc aagacagcaa caagatcgcc gtgcccaagg tgagcggcct 1140

gcagtacaga gtgttcagag tgaagctgcc cgaccccaac aagttcggct tccccgacac 1200

tagcttctat gacccggcta gccaaagact ggtgtgggcg tgtacgggcg tggaggtagg 1260

tagagggcag ccactgggcg tgggcatcag cggccacccc ctgctgaaca agctggacga 1320

caccgagaac agcaacaagt acgtgggcaa cagcggcacc gacaacagag agtgcatcag 1380

catggactac aagcagacac agctgtgcct gatcggctgc agacccccca tcggcgagca 1440

ctggggcaaa ggcaccccgt gtaacgctaa ccaagtcaag gcgggagagt gcccccccct 1500

ggagctgctg aacaccgtgc tgcaagacgg cgacatggtg gacaccggct tcggcgccat 1560

ggacttcacc accctgcaag ccaacaagag cgacgtgccc ctggacatct gcagcagcat 1620

ctgcaagtac cccgactacc tgaagatggt gagcgagccc tacggcgaca tgctgttctt 1680

ctacctgaga agagagcaga tgttcgtgag acacctgttc aacagagccg gcaccgtggg 1740

cgagaccgtg cccgccgacc tgtacatcaa gggcaccacc ggcaccctgc ctagcacaag 1800

ctacttcccc acgcctagcg gcagcatggt gacaagcgac gctcagatct tcaacaagcc 1860

ctactggctg cagagagccc aaggccacaa caacggcatc tgctggagca atcagctgtt 1920

cgtgaccgtg gtggacacca caagaagcac caacatgagc gtgtgcagcg ccgtgagcag 1980

cagcgacagc acctacaaga acgacaactt caaggagtac ctgagacacg gcgaggagta 2040

cgacctgcag ttcatctttc agctgtgcaa gatcaccctg accgccgacg tgatgaccta 2100

catccacagc atgaacccta gcatcctgga ggactggaac ttcggcctga ccccccctcc 2160

tagcggcacc ctggaggaca cctacagata cgtgacaagc caagccgtga cctgtcagaa 2220

gcctagcgcc cccaagccca aggacgaccc cctgaagaac tacaccttct gggaggtgga 2280

cctgaaggag aagttcagcg ccgacctgga tcagttcccc ctgggcagaa agttcctgct 2340

gcaagccggc ctgaaggcta gacccaactt cagactgggc aagagagccg cccccgctag 2400

cacaagcaag aagagcagca ccaagagaag aaaggtgaag agctaataga agcttaagtt 2460

taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 2520

cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 2580

aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 2640

aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 2700

ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 2760

gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 2820

ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 2880

gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 2940

ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 3000

gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 3060

tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 3120

tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 3180

aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 3240

aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 3300

cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 3360

cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 3420

ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 3480

gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 3540

tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 3600

agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 3660

ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 3720

cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 3780

agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 3840

cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 3900

atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 3960

tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 4020

acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 4080

gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 4140

cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 4200

tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 4260

tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 4320

cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 4380

ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 4440

gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 4500

gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 4560

ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 4620

aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 4680

catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 4740

cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 4800

tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 4860

cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 4920

gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 4980

tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 5040

acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 5100

aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 5160

cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 5220

gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 5280

tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 5340

tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 5400

cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 5460

gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 5520

ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 5580

ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 5640

ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga 5700

aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 5760

gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 5820

cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 5880

gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 5940

tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 6000

ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 6060

ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 6120

atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 6180

cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 6240

tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 6300

aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 6360

tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 6420

ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 6480

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 6540

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 6600

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 6660

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 6720

gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 6780

cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 6840

ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc 6882

<210> 11

<211> 6882

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 11

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

gtttaaacgg gccctctaga ctcgagcggc cgccaccatg agcctgtgga gaagcaatga 960

ggccacagtg tacctgcccc ctgtgtctgt gagcaaggtg gtgagcacag atgagtatgt 1020

gacaagaacc aacatctact accatgctgg cagcagcaga ctgctggctg tgggccaccc 1080

ctactatgcc atcaagaagc aagacagcaa caagattgct gtgcccaagg tgtctggcct 1140

gcagtacaga gtgttcagag tgaagctgcc tgaccccaac aagtttggct tccctgacac 1200

tagcttctat gaccctgcta gccaaagact ggtgtgggcc tgtactgggg tggaggtagg 1260

tagagggcag ccactggggg tgggcatctc tggccacccc ctgctgaaca agctggatga 1320

cacagagaac agcaacaagt atgtgggcaa ctctggcaca gacaacagag agtgcatcag 1380

catggactac aagcagacac agctgtgcct gattggctgc agacccccca ttggggagca 1440

ctggggcaaa ggcaccccct gtaatgccaa ccaagtcaag gctggagagt gcccccccct 1500

ggagctgctg aacacagtgc tgcaagatgg ggacatggtg gacactggct ttggggccat 1560

ggacttcacc accctgcaag ccaacaagtc tgatgtgccc ctggacatct gcagcagcat 1620

ctgcaagtac cctgactacc tgaagatggt gtctgagccc tatggggaca tgctgttctt 1680

ctacctgaga agagagcaga tgtttgtgag acacctgttc aacagagctg gcacagtggg 1740

ggagacagtg cctgctgacc tgtacatcaa gggcaccact ggcaccctgc ctagcacaag 1800

ctacttcccc accccctctg gcagcatggt gacctctgat gctcagatct tcaacaagcc 1860

ctactggctg cagagagccc aaggccacaa caatggcatc tgctggagca atcagctgtt 1920

tgtgacagtg gtggacacca caagaagcac caacatgtct gtgtgctctg ctgtgagcag 1980

ctctgacagc acctacaaga atgacaactt caaggagtac ctgagacatg gggaggagta 2040

tgacctgcag ttcatctttc agctgtgcaa gatcaccctg acagctgatg tgatgaccta 2100

catccacagc atgaacccta gcatcctgga ggactggaac tttggcctga ccccccctcc 2160

ctctggcacc ctggaggaca cctacagata tgtgacaagc caagctgtga cctgtcagaa 2220

gccctctgcc cccaagccca aggatgaccc cctgaagaac tacaccttct gggaggtgga 2280

cctgaaggag aagttctctg ctgacctgga tcagttcccc ctgggcagaa agttcctgct 2340

gcaagctggc ctgaaggcta gacccaactt cagactgggc aagagagctg cccctgctag 2400

cacaagcaag aagagcagca ccaagagaag aaaggtgaag agctaataga agcttaagtt 2460

taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 2520

cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 2580

aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 2640

aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 2700

ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 2760

gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 2820

ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 2880

gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 2940

ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 3000

gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 3060

tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 3120

tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 3180

aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 3240

aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 3300

cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 3360

cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 3420

ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 3480

gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 3540

tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 3600

agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 3660

ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 3720

cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 3780

agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 3840

cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 3900

atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 3960

tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 4020

acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 4080

gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 4140

cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 4200

tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 4260

tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 4320

cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 4380

ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 4440

gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 4500

gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 4560

ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 4620

aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 4680

catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 4740

cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 4800

tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 4860

cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 4920

gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 4980

tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 5040

acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 5100

aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 5160

cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 5220

gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 5280

tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 5340

tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 5400

cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 5460

gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 5520

ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 5580

ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 5640

ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga 5700

aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 5760

gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 5820

cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 5880

gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 5940

tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 6000

ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 6060

ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 6120

atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 6180

cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 6240

tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 6300

aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 6360

tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 6420

ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 6480

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 6540

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 6600

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 6660

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 6720

gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 6780

cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 6840

ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc 6882

<210> 12

<211> 6783

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 12

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

gtttaaacgg gccctctaga ctcgagcggc cgccaccatg cgccacaagc ggtccaccaa 960

gagagtgaag agggcctctg ccacacagct gtacagaacc tgcaaggcag caggaacatg 1020

tccacctgac gtgatcccca aggtggaggg caacaccgtg gccgatcaga tcctgaagta 1080

cggctctatg gccgtgttct ttggcggcct gggcatcgga tctggaagcg gaaccggcgg 1140

ccggagcggc tatgtgccac tgggaaccac accaccaaca gcagcaacca atatccccat 1200

ccggcctcca gtgacagtgg agtccatccc cctggacacc atcggccctc tggatagctc 1260

catcgtgtct ctggtggagg agacatcctt catcgagtct ggagcacctg tggtgacccc 1320

aagggtgcca cctaccacag gctttacaat caccacatcc accgacacca cacctgccat 1380

cctggatgtg acatccatct ctacccacga caacccaacc ttcacagatc catctgtgct 1440

gcacccacca acaccagcag agaccagcgg ccactttgtg ctgtctagct cctctatctc 1500

cacccacaac tacgaggaga tccctatgga cacattcatc gtgtctaccg atagcaacaa 1560

tatcacaaat agcaccccta tcccaggctc cagacctacc acaaggctgg gcctgtatag 1620

caagggcacc cagcaggtga aggtggtgga cccagccttt atgacatccc ccgccaagct 1680

gatcacctac gataaccccg cctatgaggg cctgaatcca gacaccacac tgcagttcga 1740

gcacgaggat atctccctgg cccccgaccc tgacttcatg gacatcatcg ccctgcacag 1800

accagccctg acaagcagga agggcaccat ccgctactcc cgggtgggca acaagcgcac 1860

aatgcacacc cggagcggca aggcaatcgg agcaagggtg cactactatc aggacctgag 1920

ctccatcacc gaggatatcg agctgcagcc actgcagcac gtgccctcta gcctgcctca 1980

caccacagtg agcacatccc tgaatgacgg catgttcgat atctacgccc ccatcgacac 2040

cgaggaggat atcatctttt ccgcctcctc taacaataca ctgtacacca catctaacac 2100

cgcctatgtg cctagcaata ccacaatccc actgagctcc ggctatgaca tccctatcac 2160

agccggccca gatatcgtgt tcaacagcaa taccatcaca aataccgtgc tgccagtgcc 2220

aaccggacca atctactcta tcatcgcaga cggaggcgac ttctacctgc accctagcta 2280

ctatctgctg aagcggagaa ggaagcggat cccatatttc tttgccgacg tgagcgtggc 2340

cgtgtaatag aagcttaagt ttaaaccgct gatcagcctc gactgtgcct tctagttgcc 2400

agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca 2460

ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta 2520

ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc 2580

atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta 2640

gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 2700

gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 2760

cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 2820

ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 2880

cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 2940

tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 3000

cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 3060

aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc 3120

cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 3180

gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 3240

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 3300

cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 3360

ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 3420

caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg 3480

atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 3540

gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 3600

ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 3660

gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 3720

cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 3780

gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 3840

tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 3900

gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 3960

tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 4020

catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 4080

ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 4140

ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 4200

tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 4260

tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 4320

acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 4380

ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 4440

gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat 4500

agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 4560

aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg 4620

taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 4680

atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 4740

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 4800

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 4860

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 4920

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 4980

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 5040

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 5100

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 5160

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 5220

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 5280

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 5340

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 5400

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 5460

tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 5520

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc 5580

aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 5640

gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 5700

aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 5760

atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 5820

gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 5880

atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 5940

ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 6000

cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 6060

agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 6120

cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 6180

tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 6240

agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 6300

gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 6360

gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 6420

ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 6480

tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 6540

tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 6600

gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 6660

caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 6720

atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 6780

gtc 6783

<210> 13

<211> 6783

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 13

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

gtttaaacgg gccctctaga ctcgagcggc cgccaccatg agacacaaga gaagcaccaa 960

gagagtgaag agagctagcg ccacacagct gtacagaacc tgcaaggccg ccggcacctg 1020

cccccccgac gtgatcccca aggtggaggg caacaccgtg gccgatcaga tcctgaagta 1080

tggcagcatg gctgtcttct tcggcggcct gggtatcggc agcggaagcg gcaccggcgg 1140

cagaagtggg tatgtgcccc ttggtacgac accccccacc gccgccacca acatccccat 1200

cagacccccc gtgaccgtgg agagcatccc cctggacacc atcggccccc tggacagcag 1260

catcgtgagc ctggtggagg agactagctt catcgagagc ggcgcccccg tggtgacccc 1320

tagagtgccc cccaccaccg gcttcaccat caccacaagc acggacacga cacccgccat 1380

cctggacgtg acctccatca gcactcacga taaccctacc tttaccgacc ctagcgttct 1440

gcatcccccc acgcccgccg agacaagcgg ccacttcgtg ctgagcagca gcagcatcag 1500

cacccacaac tatgaggaga tccccatgga caccttcatc gtgagcaccg acagcaacaa 1560

catcacaaac tccacaccca ttcccggcag cagacccacc acaagactgg gcctgtacag 1620

caagggcaca cagcaagtga aggtggtgga ccccgccttc atgacaagcc ccgccaagct 1680

gatcacctac gacaaccccg cctacgaggg cctgaacccc gacacaacgc tgcagttcga 1740

gcacgaggac atcagcctgg cccccgaccc cgacttcatg gacatcatcg ccctgcacag 1800

acccgccctg acaagcagaa agggcaccat cagatacagc agagtgggca acaagagaac 1860

catgcacaca agaagcggca aggccatcgg cgctagagtg cactactacc aagacctgag 1920

cagcatcacc gaggacatcg agctgcagcc cctgcagcac gttccctcgt ccctgcccca 1980

caccaccgtg agcacaagcc tgaacgacgg catgttcgac atctacgccc ccatcgacac 2040

cgaggaggac atcatcttca gcgctagcag caacaacacc ctgtacacca caagcaacac 2100

cgcctacgtg cctagcaaca ccaccatccc cctgagcagc ggctacgaca tccccataac 2160

tgccggcccc gacatcgtgt tcaacagcaa caccatcacc aacaccgtgc tgcccgtgcc 2220

caccggcccc atctacagca tcatcgccga cggcggcgac ttctacctgc accctagcta 2280

ctacctgctg aagagaagaa gaaagagaat cccctacttc ttcgccgacg tgagcgtggc 2340

cgtgtaatag aagcttaagt ttaaaccgct gatcagcctc gactgtgcct tctagttgcc 2400

agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca 2460

ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta 2520

ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc 2580

atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta 2640

gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 2700

gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 2760

cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 2820

ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 2880

cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 2940

tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 3000

cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 3060

aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc 3120

cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 3180

gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 3240

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 3300

cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 3360

ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 3420

caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg 3480

atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 3540

gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 3600

ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 3660

gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 3720

cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 3780

gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 3840

tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 3900

gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 3960

tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 4020

catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 4080

ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 4140

ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 4200

tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 4260

tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 4320

acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 4380

ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 4440

gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat 4500

agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 4560

aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg 4620

taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 4680

atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 4740

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 4800

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 4860

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 4920

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 4980

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 5040

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 5100

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 5160

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 5220

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 5280

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 5340

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 5400

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 5460

tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 5520

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc 5580

aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 5640

gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 5700

aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 5760

atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 5820

gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 5880

atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 5940

ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 6000

cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 6060

agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 6120

cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 6180

tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 6240

agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 6300

gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 6360

gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 6420

ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 6480

tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 6540

tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 6600

gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 6660

caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 6720

atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 6780

gtc 6783

<210> 14

<211> 6783

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 14

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

gtttaaacgg gccctctaga ctcgagcggc cgccaccatg agacacaaga gaagcaccaa 960

gagagtgaag agagcctctg ccacacagct gtacagaacc tgcaaggctg ctggcacctg 1020

cccccctgat gtgatcccca aggtggaggg caacacagtg gctgatcaga tcctgaagta 1080

tggcagcatg gctgtcttct ttgggggcct gggtattggc tctggctctg gcactggggg 1140

cagaagtggg tatgtgcccc ttggtaccac accccccaca gctgccacca acatccccat 1200

cagaccccct gtgacagtgg agagcatccc cctggacacc attggccccc tggacagcag 1260

cattgtgagc ctggtggagg agactagctt cattgagtct ggggcccctg tggtgacccc 1320

tagagtgccc cccaccactg gcttcaccat caccacaagc acagacacca cacctgccat 1380

cctggatgtg acctccatca gcactcatga caaccctacc tttacagacc cctctgtgct 1440

gcatcccccc acccctgctg agacctctgg ccactttgtg ctgagcagca gcagcatcag 1500

cacccacaac tatgaggaga tccccatgga caccttcatt gtgagcacag acagcaacaa 1560

catcacaaac tccacaccca ttcctggcag cagacccacc acaagactgg gcctgtacag 1620

caagggcaca cagcaagtga aggtggtgga ccctgccttc atgacaagcc ctgccaagct 1680

gatcacctat gacaaccctg cctatgaggg cctgaaccct gacacaaccc tgcagtttga 1740

gcatgaggac atcagcctgg cccctgaccc tgacttcatg gacatcattg ccctgcacag 1800

acctgccctg acaagcagaa agggcaccat cagatacagc agagtgggca acaagagaac 1860

catgcacaca agatctggca aggccattgg ggctagagtg cactactacc aagacctgag 1920

cagcatcaca gaggacattg agctgcagcc cctgcagcat gtgcctagct ccctgcccca 1980

caccacagtg agcacaagcc tgaatgatgg catgtttgac atctatgccc ccattgacac 2040

agaggaggac atcatcttct ctgctagcag caacaacacc ctgtacacca caagcaacac 2100

agcctatgtg cctagcaaca ccaccatccc cctgagctct ggctatgaca tccccataac 2160

tgctggccct gacattgtgt tcaacagcaa caccatcacc aacacagtgc tgcctgtgcc 2220

cactggcccc atctacagca tcattgctga tgggggggac ttctacctgc accctagcta 2280

ctacctgctg aagagaagaa gaaagagaat cccctacttc tttgctgatg tgtctgtggc 2340

tgtgtaatag aagcttaagt ttaaaccgct gatcagcctc gactgtgcct tctagttgcc 2400

agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca 2460

ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta 2520

ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc 2580

atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta 2640

gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 2700

gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 2760

cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 2820

ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 2880

cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 2940

tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 3000

cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 3060

aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc 3120

cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 3180

gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 3240

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 3300

cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 3360

ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 3420

caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg 3480

atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 3540

gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 3600

ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 3660

gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 3720

cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 3780

gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 3840

tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 3900

gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 3960

tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 4020

catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 4080

ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 4140

ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 4200

tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 4260

tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 4320

acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 4380

ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 4440

gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat 4500

agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 4560

aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg 4620

taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 4680

atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 4740

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 4800

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 4860

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 4920

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 4980

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 5040

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 5100

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 5160

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 5220

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 5280

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 5340

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 5400

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 5460

tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 5520

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc 5580

aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 5640

gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 5700

aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 5760

atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 5820

gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 5880

atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 5940

ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 6000

cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 6060

agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 6120

cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 6180

tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 6240

agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 6300

gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 6360

gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 6420

ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 6480

tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 6540

tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 6600

gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 6660

caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 6720

atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 6780

gtc 6783

<210> 15

<211> 6093

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 15

gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

gtttaaacgg gccctctaga ctcgagcggc cgccaccatg gtgagcaagg gcgaggagct 960

gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt 1020

cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 1080

ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg 1140

cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 1200

catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 1260

gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 1320

catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag 1380

ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat 1440

ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 1500

catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 1560

gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 1620

cgggatcact ctcggcatgg acgagctgta caagtaatag aagcttaagt ttaaaccgct 1680

gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 1740

cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 1800

catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 1860

agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt 1920

ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg 1980

cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 2040

tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 2100

gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 2160

accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 2220

tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 2280

gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 2340

cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg 2400

gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 2460

aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 2520

cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 2580

gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 2640

tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg 2700

aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat 2760

tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 2820

gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 2880

gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 2940

ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 3000

atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 3060

gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 3120

tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 3180

tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 3240

gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 3300

agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 3360

ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 3420

cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 3480

tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 3540

cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 3600

actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 3660

tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 3720

atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt 3780

gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 3840

ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt 3900

ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 3960

aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 4020

tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 4080

cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 4140

ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 4200

cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 4260

ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 4320

aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 4380

cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 4440

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 4500

gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 4560

tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 4620

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 4680

ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 4740

gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 4800

gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 4860

accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 4920

tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 4980

cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 5040

taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 5100

caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 5160

gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 5220

gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 5280

ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 5340

attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 5400

gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 5460

tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 5520

agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 5580

gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 5640

actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 5700

tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 5760

attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 5820

tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 5880

tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 5940

aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 6000

tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 6060

cgcacatttc cccgaaaagt gccacctgac gtc 6093

Claims (10)

1. The coding genes of human papillomavirus type 35L1 protein and L2 protein are characterized in that the nucleotide sequence of the coding gene of the L1 protein is shown as SEQ ID NO.4 or SEQ ID NO. 5; the nucleotide sequence of the L2 protein coding gene is shown in SEQ ID NO.7 or SEQ ID NO. 8.

2. A recombinant expression vector comprising the coding gene of claim 1.

3. The recombinant expression vector of claim 2, further comprising a reporter gene coding sequence.

4. A host cell comprising or incorporating the recombinant expression vector of claim 2.

5. Use of the genes encoding human papillomavirus type 35L1 protein and L2 protein according to claim 1, the recombinant expression vector according to claim 2, or the host cell according to claim 4 for the preparation of human papillomavirus type 35 pseudoviruses.

6. A construction method of human papilloma virus type 35 pseudovirus comprises the steps of using a sequence shown in a nucleotide sequence SEQ ID NO.4 or SEQ ID NO.5 to code L1 protein and a sequence shown in a nucleotide sequence SEQ ID NO.7 or SEQ ID NO.8 to code L2 protein.

7. The method according to claim 6, characterized in that it comprises the steps of:

1) constructing a recombinant expression vector of a coding gene of human papillomavirus type 35L1 protein containing a sequence shown in a nucleotide sequence SEQ ID NO.4 or SEQ ID NO.5 and a recombinant expression vector of a coding gene of human papillomavirus type 35L2 protein containing a sequence shown in a nucleotide sequence SEQ ID NO.7 or SEQ ID NO. 8;

2) culturing and expressing the two expression vectors in a host cell;

3) the host cells are lysed and the protein of interest is collected.

8. The method of claim 7, wherein both of said recombinant expression vectors further comprise a reporter gene.

9. The method of claim 8, wherein the reporter protein is a fluorescent protein.

10. The method according to claim 9, wherein the fluorescent protein is selected from any one or more of green fluorescent protein, red fluorescent protein, yellow fluorescent protein and blue fluorescent protein.

Images