CN112674114A - Nano-silver antibacterial composition and preparation method thereof - Google Patents
Nano-silver antibacterial composition and preparation method thereof Download PDFInfo
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- CN112674114A CN112674114A CN202011418896.7A CN202011418896A CN112674114A CN 112674114 A CN112674114 A CN 112674114A CN 202011418896 A CN202011418896 A CN 202011418896A CN 112674114 A CN112674114 A CN 112674114A
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Abstract
The invention relates to the field of materials, and particularly discloses a nano-silver antibacterial composition and a preparation method thereof. The invention optimizes the preparation method of the nano-silver and searches for the minimum inhibitory concentration, thereby reducing the content of silver ions, improving the safety and solving the technical problem of high production cost in the prior art.
Description
Technical Field
The invention relates to the field of materials, in particular to a nano-silver antibacterial composition and a preparation method thereof.
Background
AgNP (nano silver particles) has excellent antimicrobial properties and reacts against a variety of microorganisms including bacteria, fungi and viruses for the purpose of sterilization. In addition, the metallic silver is an effective antibacterial agent and has high antibacterial potential. Meanwhile, silver-based preservatives have a wide range of applications, resulting in a low possibility of the microorganisms developing resistance to the preservatives, and thus silver ions are widely studied.
Silver ions have the ability to inhibit bacterial replication by binding and denaturing bacterial DNA, which reacts with thiol groups of proteins, followed by DNA condensation leading to apoptosis. The nano-form of silver is more reactive with thiol groups of proteins and they cross cell membranes, resulting in deposition of nanoparticles within cells, thereby causing cell dysfunction, and most of the existing studies apply nano-silver to bactericidal products.
The patent CN111603422A discloses a no-clean antibacterial hand sanitizer and a preparation method thereof, 0.01-0.03 part of silver, 0.02-0.08 part of silver nitrate, 1-5 parts of humectant, 10-18 parts of skin conditioner, 0.3-0.8 part of preservative, 0.1-0.5 part of sodium dodecyl benzene sulfonate, 0.8-1.8 parts of ethylhexyl glycerol, 1-5 parts of propylene glycol, 143-6 parts of nonylphenol polyether, 1-6 parts of PEG-50 castor oil, 0.01-0.05 part of essence and 80-120 parts of deionized water; chinese patent CN108142420A adopts zinc nitrate or copper acetate solution to react with precursor solution containing cellulose and organic ligand, and metal ions and the organic ligand are synthesized on the cellulose in situ by an in-situ synthesis method to obtain a cellulose-metal organic framework compound, and the compound has good antibacterial effect; chinese patent CN108837159A adopts a mixed solution of zinc nitrate hexahydrate and antibiotic to react with a 2-methylimidazole solution to prepare an MOF-antibiotic synergistic system, and sodium hyaluronate is added to obtain a nano antibacterial agent; chinese patent CN111955479A provides a silver-containing antibacterial liquid, which comprises: the Ag-MOF/nano silver composite comprises Ag-MOF/nano silver composite and a stabilizer coating the composite, wherein the mass concentration of the Ag-MOF/nano silver composite in the silver-containing antibacterial liquid is 0.01-0.5%, and the mass concentration of the stabilizer in the silver-containing antibacterial liquid is 0.002-0.005%. However, these products have high silver ion content, high production cost, complex components and limited antibacterial effect.
How to safely and conveniently improve nano-silver drugs under the condition of controlling production cost, and search for minimum bacteriostatic concentration while ensuring the bactericidal capacity of the nano-silver drugs, thereby reducing the content of silver ions, and becoming a problem to be solved urgently.
Disclosure of Invention
The invention aims to solve the problems and provides a nano-silver antibacterial product, which reduces the silver content and ensures the antibacterial effect of the product by optimizing the preparation method of the nano-silver.
One of the purposes of the invention is to provide a nano-silver antibacterial composition, which reduces the content of silver ions, controls the production cost, improves the safety and simultaneously ensures the antibacterial effect of the product, and the specific technical scheme is as follows:
a nanometer silver antibacterial composition comprises silver nitrate, tannic acid, curcumin, ferric chloride hexahydrate, dimethyl sulfoxide, bovine serum albumin and deionized water; the weight ratio of the silver nitrate, the tannic acid, the curcumin, the ferric trichloride hexahydrate, the dimethyl sulfoxide and the bovine serum albumin is 1: 60: 6: 12: 1200: 120.
the second purpose of the invention is to provide the application of the nano-silver antibacterial composition in the scheme in the preparation of antibacterial products.
Such as in the preparation of antibacterial hand sanitizers, antibacterial dressings, and antibacterial solutions.
The third purpose of the invention is to provide a method for preparing an antibacterial solution by using the nano-silver antibacterial composition in the scheme, which comprises the following specific technical scheme:
a method for preparing an antibacterial solution from a nano-silver antibacterial composition comprises the following steps:
(1) dissolving bovine serum albumin in the composition in deionized water to obtain bovine serum albumin solution;
(2) sequentially adding ferric trichloride hexahydrate solution and silver nitrate solution into bovine serum albumin solution, and stirring for dispersion;
(3) adding tannic acid-curcumin mixed solution to obtain nano silver antibacterial solution.
Further, the concentration of the ferric trichloride hexahydrate solution in the step (2) is 0.5mg/ml, and the concentration of the silver nitrate is 0.01 mol/L.
Further, the time for stirring and dispersing in the step (2) is 30 min.
Further, the concentration ratio of tannic acid to turmeric in the tannic acid-turmeric solution in step (3) is 10: 1.
further, the tannic acid-curcumin mixed solution is formed by mixing a tannic acid solution with the concentration of 50mg/ml and a curcumin solution with the concentration of 5 mg/ml.
Further, the tannic acid solution is prepared by dissolving tannic acid in deionized water, and the curcumin solution is prepared by dissolving curcumin in dimethyl sulfoxide.
The fourth purpose of the invention is to apply for protection of the nano-silver antibacterial solution prepared by any one of the preparation methods.
The fifth purpose of the invention is to apply the nano-silver antibacterial solution prepared by the scheme to kill escherichia coli and/or staphylococcus aureus bacteria, and the concentration ratio of the nano-silver antibacterial solution to the escherichia coli and/or staphylococcus aureus bacteria is more than 0.02.
The invention has the advantages that: the preparation method of the nano-silver antibacterial solution is simple, reduces the silver content, has the minimum bactericidal concentration ratio of 0.02, reduces the production cost, improves the safety and ensures the antibacterial effect of the product.
Drawings
Fig. 1 is a transmission electron microscope image of the nano silver antibacterial solution of the present invention.
Fig. 2 is a DLS result graph of the nano silver antibacterial solution of the present invention.
FIG. 3 is a graph showing the antibacterial effect of the nano-silver antibacterial solution of the present invention on agar plates of Escherichia coli and Staphylococcus aureus at different concentrations.
Fig. 4 is a bacteriostatic concentration-absorbance diagram of the nano-silver antibacterial solution of the present invention.
In the figure, Ct represents a control group, namely, the nano-silver antibacterial solution is not added, and only bacteria are contained; 1 represents a mixture of 0.1ul of nano-silver antibacterial solution and 100ul of bacteria; 2 represents a mixture of 0.3ul of nano-silver antibacterial solution and 100ul of bacteria; 3 represents a mixture of 0.5ul of nano-silver antibacterial solution and 100ul of bacteria; 4 represents a mixture of 1ul of nano-silver antibacterial solution and 100ul of bacteria; 5 represents a mixture of 3ul of nano-silver antibacterial solution and 100ul of bacteria; 6 represents a mixture of 5ul of nano-silver antibacterial solution and 100ul of bacteria; 7 represents a mixture of 10ul of nano-silver antibacterial solution and 100ul of bacteria.
Detailed Description
The present invention will be described in further detail with reference to the following examples and drawings, it being understood that the present invention is not limited to the particular examples described herein, but is capable of modification in various forms and details, and can be modified within the spirit and scope of the invention.
Example 1 Nano silver antimicrobial product
A nanometer silver antibacterial product comprises silver nitrate, tannic acid, curcumin, ferric chloride hexahydrate, dimethyl sulfoxide, bovine serum albumin, and deionized water; wherein the weight ratio of silver nitrate, tannic acid, curcumin, ferric trichloride hexahydrate, dimethyl sulfoxide and bovine serum albumin is 1: 60: 6: 12: 1200: 120.
example 2 preparation method of nano silver antibacterial solution
(1) Preparation of related solutions
Weighing 5mg ferric trichloride hexahydrate powder, and dissolving the ferric trichloride hexahydrate powder in 10ml deionized water to prepare a ferric trichloride hexahydrate solution with the concentration of 0.5 mg/ml;
weighing 1.6987g of silver nitrate, and dissolving the silver nitrate in 1L of deionized water to prepare 0.01mol/L of silver nitrate solution;
500mg of tannic acid powder is weighed and dissolved in 10ml of deionized water to prepare a tannic acid solution with the concentration of 50 mg/ml:
50mg of curcumin powder was weighed and dissolved in 10ml of dimethyl sulfoxide to prepare a 5mg/ml curcumin solution.
(2) Preparation of nano silver antibacterial solution
Weighing 8.75mg of bovine serum albumin, dissolving the bovine serum albumin in 17.5ml of deionized water, uniformly stirring, sequentially adding 2ml of ferric trichloride hexahydrate solution with the concentration of 0.5mg/ml and 0.5ml of silver nitrate solution with the concentration of 0.01mol/l, stirring for 30min to be uniformly dispersed, dropwise adding a mixture of 100ul of tannic acid with the concentration of 50mg/ml and 100ul of curcumin solution with the concentration of 5mg/ml, continuously performing magnetic stirring until all the tannic acid-curcumin mixed solution is dropwise added, and then closing a magnetic stirrer to obtain the nano-silver antibacterial solution, wherein a transmission electron microscope picture of the nano-silver antibacterial solution is shown in figure 1, the morphology and the size of the nano-silver are represented, and the size of nano-silver particles in a projection electron microscope is about 40 nm. DLS in FIG. 2 shows a hydrodynamic diameter of 70.28, which is normal for results larger than transmission electron microscopy. The smaller the nano-particles, the larger the specific surface area, the larger the contact range with bacteria and the stronger killing effect on the bacteria.
(2) Comparison of the bactericidal effects of different concentrations of the nano-silver antibacterial solutions
| Numbering | Volume of germicidal solution (same concentration) |
| |
0 |
| 1 | 0.1 |
| 2 | 0.3 |
| 3 | 0.5ul |
| 4 | 1ul |
| 5 | |
| 6 | 5ul |
| 7 | 10ul |
Ct is experimental control group; wherein the mixture of bacteria is a mixture solution with the same concentration and the volume of 100 ul; the bacterial species are escherichia coli and/or staphylococcus aureus.
Fig. 3 shows the antibacterial effect of the product on agar plates of escherichia coli and staphylococcus aureus under different concentrations, and when the product is combined with fig. 4, it can be seen that the bacteria of the experimental groups with numbers 5-7 are completely killed, namely the volume of the nano-silver antibacterial solution for completely killing the bacteria at the lowest is 3ul, and the complete sterilization effect is achieved when 3ul of the nano-silver antibacterial solution is mixed with 100ul of escherichia coli or staphylococcus aureus. 3ul of nano silver solution comprises the following components: the silver nitrate is 1.274025 × 10-8g, bovine serum albumin is 1.5 × 10-6g, ferric trichloride hexahydrate of 1.5X 10-7g, tannic acid 7.5X 10-7g, curcumin is 7.5 × 10-8g, dimethyl sulfoxide is 1.5X 10-5g, and the balance of deionized water.
It can be seen from (c) and (d) of fig. 4 that the nano-silver antibacterial solution has the minimum inhibitory concentration when the volume of the nano-silver antibacterial solution is 2 ul. The content of each component of the nano silver solution when the silver content is 2ul is as follows: the silver nitrate is 8.4935 × 10-9g, bovine serum albumin is 1.0 × 10-6g, ferric trichloride hexahydrate of 1.0X 10-7g, tannic acid 5.0 × 10-7g, curcumin is 5.0 × 10-8g, dimethyl sulfoxide is 1.0X 10-5g, and the balance of deionized water.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. A nano-silver antibacterial composition is characterized by comprising silver nitrate, tannic acid, curcumin, ferric trichloride hexahydrate, dimethyl sulfoxide, bovine serum albumin and deionized water; the weight ratio of the silver nitrate, the tannic acid, the curcumin, the ferric trichloride hexahydrate, the dimethyl sulfoxide and the bovine serum albumin is 1: 60: 6: 12: 1200: 120.
2. the use of the nano-silver antibacterial composition of claim 1 in the preparation of antibacterial products.
3. A method for preparing a nano silver antibacterial solution using the nano silver antibacterial composition of claim 1, comprising the steps of:
(1) dissolving bovine serum albumin in the composition in deionized water to obtain bovine serum albumin solution;
(2) sequentially adding ferric trichloride hexahydrate solution and silver nitrate solution into bovine serum albumin solution, and stirring for dispersion;
(3) adding tannic acid-curcumin mixed solution to obtain nano silver antibacterial solution.
4. The method according to claim 3, wherein the concentration of the ferric trichloride hexahydrate solution in the step (2) is 0.5mg/ml, and the concentration of the silver nitrate is 0.01 mol/l.
5. The method according to claim 3, wherein the stirring dispersion time in the step (2) is 30 min.
6. The method of claim 3, wherein the concentration ratio of tannic acid to turmeric in the tannic-turmeric solution of step (3) is from 10: 1.
7. the method according to claim 3, wherein the tannin-curcumin mixed solution is prepared by mixing a tannin solution with a concentration of 50mg/ml and a curcumin solution with a concentration of 5 mg/ml.
8. The method of claim 7, wherein the tannin solution is prepared by dissolving tannin in deionized water, and the curcumin solution is prepared by dissolving curcumin in dimethyl sulfoxide.
9. The nano-silver antibacterial solution prepared by the method of any one of claims 3 to 8.
10. The use of the nano-silver antibacterial solution of claim 9 for killing escherichia coli and/or staphylococcus aureus, wherein the concentration ratio of the nano-silver antibacterial solution to the escherichia coli and/or staphylococcus aureus is more than 0.02.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1876293A (en) * | 2006-04-03 | 2006-12-13 | 庄平 | Nano-class silver colloidal solution and preparation method thereof |
| CN101147977A (en) * | 2007-10-17 | 2008-03-26 | 楚雄师范学院 | Method for preparing high activity and long service negative charged colloidal nanometer silver |
| CN109673636A (en) * | 2019-01-15 | 2019-04-26 | 南京工业大学 | A method of nuclear shell structure nano silver anti-biotic material is prepared based on plant polyphenol |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1876293A (en) * | 2006-04-03 | 2006-12-13 | 庄平 | Nano-class silver colloidal solution and preparation method thereof |
| CN101147977A (en) * | 2007-10-17 | 2008-03-26 | 楚雄师范学院 | Method for preparing high activity and long service negative charged colloidal nanometer silver |
| CN109673636A (en) * | 2019-01-15 | 2019-04-26 | 南京工业大学 | A method of nuclear shell structure nano silver anti-biotic material is prepared based on plant polyphenol |
Non-Patent Citations (2)
| Title |
|---|
| 杨晓溪: "姜黄素功能化无机纳米材料的合成及其抗病原微生物活性分析", 《中国博士学位论文全文数据库 医药卫生科技辑》 * |
| 费成刚 等: "银—牛血清白蛋白纳米粒子的制备及抗菌性能", 《武汉理工大学学报》 * |
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Application publication date: 20210420 |