CN112649374A - Verification method of skin penetration efficacy of beauty product and application thereof - Google Patents
Verification method of skin penetration efficacy of beauty product and application thereof Download PDFInfo
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Abstract
The invention discloses a method for verifying the transdermal efficacy of a beauty product and application thereof, and relates to the field of verification of the transdermal efficacy of the beauty product. The verification method is simple, the actual use condition of a user can be simulated without expensive test equipment, and the transdermal effect is easy to present. The verification method can not only verify the transdermal efficacy of the product, but also reversely help research and development departments to adjust the parameters of the beauty product and develop the product with better transdermal efficacy. The parameters affecting the transdermal efficacy of cosmetic products are mainly the following: ultrasonic frequency, service time, temperature and electric field intensity, and the ultrasonic frequency, the service time, the temperature and the electric field intensity when the skin penetration effect is optimal are screened out by adjusting different ultrasonic frequencies, service times, temperatures and electric field intensities.
Description
Technical Field
The invention relates to the field of skin penetration efficacy verification of a beauty product, and particularly relates to a verification method of skin penetration efficacy of a beauty product and application thereof.
Background
In order to achieve better beautifying and skin care effects, a plurality of beautifying products are combined with the beautifying and skin care products for use at the same time, more essential components in the beautifying and skin care products are promoted to permeate into the skin by virtue of the functions of the beautifying products, for example, RF (radio frequency) acts on the skin to heat the skin, EMS (electro mechanical system (micro current) end heating function heats the test skin, electric field intensity generated by RF and EMS ends, ultrasonic frequency of ultrasonic beautifying products and the like all contribute to the permeation of the essential components. In order to verify whether the cosmetic product can promote the penetration and absorption of essence, people try to establish a method for verifying the transdermal efficacy, and only the American and Japanese have the transdermal efficacy verification method at present. The method for verifying the transdermal efficacy in the United states is the same as that in Japan, and the specific operation comprises the following steps: respectively placing test skins using a beauty product and not using the beauty product on a test fixture, soaking one surface of the test skin in essence, soaking the other surface of the test skin in physiological saline simulating body fluid of a human body to permeate the test skins, detecting the content of essence substances in the physiological saline at intervals, supplementing the physiological saline with equal volume, finishing and comparing test data of the two types of test skins after the test is finished, and if the essence ingredients in the physiological saline corresponding to the skins using the beauty product are more than those in the physiological saline corresponding to the skins not using the beauty product, indicating that the beauty product is beneficial to the permeation of the essence ingredients into the skins.
However, the above prior art testing scheme has the following disadvantages:
on one hand, the detection process is complex, the transdermal effect cannot be seen clearly in the existing test scheme, and the transdermal effect is not convenient to show for customers. On the other hand, the detection mode is complex, the detection equipment is expensive, and the working difficulty and the test cost are increased. In addition, the existing verification method is not consistent with the actual use condition of the user. The existing testing method comprises the steps that firstly, a beauty product acts on tested skin for a certain time, then one side of the tested skin is soaked in essence, and the other side of the tested skin is soaked in physiological saline simulating human body fluid for permeation; the actual use condition of a user is that after the skin is smeared with essence substances, the skin care product is used, and the essence is led into the skin by virtue of the skin care product, so that the skin can fully absorb the essence, and the effect of whitening the skin is achieved.
In view of this, the invention is particularly proposed.
Disclosure of Invention
The invention aims to provide a method for verifying the transdermal efficacy of a cosmetic product and application thereof so as to solve the technical problems.
The invention is realized by the following steps:
a method for verifying the transdermal efficacy of a cosmetic product, comprising the following steps:
treating the test skin coated with the color-developing agent with a cosmetic product, then slicing the test skin, and obtaining the transdermal efficacy of the cosmetic product according to the penetration depth of the color-developing agent of the test skin under a microscope;
the test skin was either pigskin or artificial skin.
The invention provides a method for verifying the transdermal efficacy of a beauty product, which is simple, can simulate the actual use condition of a user without expensive test equipment, and has easy presentation of the transdermal effect.
Under a microscope, the penetration depth of the color developing agent can be observed, and the skin penetration effect of the beauty product can be judged according to the penetration depth of the color developing agent.
The test skin is selected to be skin having a structure similar to that of human skin, such as pig skin or artificial skin, so that the actual use of the user can be maximally simulated. Preferably, the test skin is pig ears.
The color-developing agent is allura red, carmine, amaranth, sunset yellow, erythrosine, lemon yellow, indigo or brilliant blue. In other embodiments, the color-developing agent may be adaptively adjusted according to actual needs, for example, any one of natural pigments and synthetic pigments. In one embodiment, the developer has a molecular weight of less than 600.
In a preferred embodiment of the present invention, the cosmetic product is an ultrasonic cosmetic product, and the ultrasonic cosmetic product is at least one of the following products: roller type cosmetic instrument, ultrasonic cosmetic instrument, ion guide-in cosmetic instrument, steam type cosmetic instrument, and LED cosmetic instrument.
In other embodiments, the cosmetic product may be a commercially available ultrasonic cosmetic apparatus: for example, a current and electric pulse system, a micro-vibration system, a color light irradiation system, a body temperature raising system, a microwave system, and a vacuum adsorption system are used.
In a preferred embodiment of the invention, the verification method comprises coating the test skin with the developer, then treating the test skin coated with the developer with the cosmetic product, and repeating the coating and cosmetic product treating steps 2-10 times in sequence.
The test skin is dry due to the volatilization of moisture in the developer during the test, so that the developer needs to be coated for multiple times and the corresponding cosmetic product treatment is carried out.
In a preferred embodiment of the present invention, the verification method further comprises placing the test skin after the last cosmetic product treatment in a set temperature environment, then washing the test skin, slicing the test skin, observing and measuring the penetration depth.
In a preferred embodiment of the present invention, the time period of the above-mentioned placing under the set temperature environment is 20-30 minutes. The set ambient temperature was 37. + -. 0.5 ℃. Since the human body temperature is typically 37 ℃, an ambient temperature simulating body temperature is set here.
In a preferred embodiment of the present invention, the above verification method further comprises performing a contrast test, and integrating the results of the contrast test to determine the transdermal efficacy of the cosmetic product, wherein the contrast test comprises directly slicing the test skin coated with the color-developing agent, and performing microscopic examination.
In a preferred embodiment of the invention, the above-mentioned control test consists in coating the test skin with the developer, then leaving the test skin coated with the developer at rest, repeating the coating and leaving steps 2-10 times in sequence, and then carrying out microscopic observation.
The sectioning and microscopic observation steps were the same as those of the cosmetic treatment test group.
In a preferred embodiment of the invention, the rest time is equal to the treatment time of the cosmetic product. This is arranged to ensure that the permeation times are equal.
It should be noted that when a certain color developer has a fixed penetration depth standard for a certain test skin, it is considered that no control test is performed, and only a cosmetic product treatment is performed; in one embodiment, the control test and the cosmetic product treatment test may be performed simultaneously.
In one embodiment, the verification method comprises coating the test skin with the beauty care product having the color-developing agent dissolved therein, treating the test skin coated with the color-developing agent and the beauty care product with the beauty product, slicing the test skin, and obtaining the transdermal efficacy of the beauty product according to the color-developing agent penetration depth of the test skin under a microscope.
Meanwhile, the beauty skin care product and the color developing agent are coated at the same time, so that the effect of the beauty product on promoting the essential components in the beauty skin care product to permeate into the skin is verified.
In other embodiments, the test skin may be coated with the beauty product, then the beauty product is applied to the test skin coated with the beauty product, the skin is sliced, the color developer is applied after the slicing, and the depth of penetration of the beauty product is checked.
The beauty skin care product comprises a product which has the functions of cleaning and caring the skin or a product which has the function of treating the problem skin.
A cleaning product: can remove color cosmetics, oil dirt and dirt on the surface layer of the skin; or removing aged cutin on the outer layer of epidermis to achieve deep cleaning effect. For example: cleansing water, facial cleanser, exfoliating cream, scrub cream and facial mask.
Maintenance products: it is characterized by protecting and nourishing skin, protecting skin from or reducing stimulation of nature, preventing erosion of chemical substances, metal ions and the like to skin, preventing loss of excessive moisture of skin, promoting blood circulation and enhancing metabolism of skin. The long-term use can make the skin soft, moist, fine and tense. For example: skin caring cream, lotion, essence, sunscreen cream, mink oil, olive oil, etc.
The treatment and maintenance product comprises: generally contains a certain medicinal component, and is characterized by strong pertinence and capability of improving and treating skin problems. For example: spot-removing cream, acne lotion, sweat-inhibiting cream, deodorant powder, etc.
A method for verifying skin penetration effect of cosmetic product is used for screening electric field intensity, optimal temperature, optimal ultrasonic frequency and optimal service time of skin penetration effect of cosmetic product.
In a preferred embodiment of the present invention, the above-mentioned optimum ultrasonic frequency for screening the optimum transdermal efficacy of the cosmetic product comprises: the number of the test skins is correspondingly set according to the number of the frequency points of the beauty products, each test skin coated with the color developing agent is matched with the corresponding frequency to carry out the treatment of the beauty products, then the test skin is sliced, and the transdermal efficacy of the beauty products is obtained under a microscope according to the penetration depth of the color developing agent of the test skin.
The verification method provided by the invention not only can verify the transdermal efficacy of the product, but also can reversely help research and development departments to adjust the parameters of the beauty product, and develop the product with better transdermal efficacy. The parameters affecting the transdermal efficacy of cosmetic products are mainly the following: ultrasonic frequency, service time, temperature and electric field intensity, and the ultrasonic frequency, the service time, the temperature and the electric field intensity when the skin penetration effect is optimal are screened out by adjusting different ultrasonic frequencies, service times, temperatures and electric field intensities.
The invention has the following beneficial effects:
the invention provides a method for verifying the transdermal efficacy of a beauty product, which is simple, can simulate the actual use condition of a user without expensive test equipment, and has easy presentation of the transdermal effect. The verification method can not only verify the transdermal efficacy of the product, but also reversely help research and development departments to adjust the parameters of the beauty product and develop the product with better transdermal efficacy. The parameters affecting the transdermal efficacy of cosmetic products are mainly the following: ultrasonic frequency, service time, temperature and electric field intensity, and the ultrasonic frequency, the service time, the temperature and the electric field intensity when the skin penetration effect is optimal are screened out by adjusting different ultrasonic frequencies, service times, temperatures and electric field intensities.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 shows the steps of the verification method provided in example 1;
FIG. 2 is a diagram illustrating test results of the verification method provided in example 1;
FIG. 3 is a flow chart of ultrasonic frequency verification provided in example 2;
FIG. 4 is a flowchart of the verification of the optimum usage time provided in example 3;
FIG. 5 is a flow chart of temperature verification provided in example 4;
FIG. 6 is a flowchart of electric field strength verification provided in example 5;
FIG. 7 is a schematic view of a test fixture in a comparative example;
fig. 8 is a flow chart of transdermal efficacy verification in the comparative example.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
The embodiment provides a method for verifying the transdermal efficacy of an RF product, and the verifying steps are shown in FIG. 1 and comprise the following steps which are carried out in sequence:
(1) 2 3cm by 4cm pig skins were obtained from the same fresh pig ear and labeled as number 1 and number 2, respectively.
(2) 0.1mL of allura red solution with the same concentration is coated on the skins of No. 1 and No. 2 at the same time under the normal temperature environment, the mass concentration of the allura red solution in the embodiment is 0.00625%, and the allura red is purchased from Rouca (Shanghai) food additive trade company Limited.
(3) 0.1mL of allura red solution with the same concentration was applied to the skin No. 1 and No. 2 simultaneously in a normal temperature environment.
(4) Skin No. 1 was left at room temperature to allow the allura red solution to freely permeate, and No. 2 used the cosmetic product effect to accelerate the allura red permeation, for a total of 3 minutes.
(5) 0.1mL of allura red solution with the same concentration was applied to the skin No. 1 and No. 2 simultaneously in a normal temperature environment.
(6) Skin No. 1 was left at ambient temperature to allow the allura red solution to freely penetrate, and skin No. 2 was tested for 3 minutes using a cosmetic product to accelerate the permeation of allura red.
(7) Test skin nos. 1 and 2 were placed in the set ambient temperature for 30 minutes (ambient temperature was selected to be 37 ℃).
(8) And (3) cleaning the No. 1 and No. 2 test skins in the step (7), and cleaning the allure red which does not permeate into the skins to avoid interfering with subsequent observation and measurement.
(9) The test skins No. 1 and No. 2 were sliced, respectively, and the depths of penetration of the test skins No. 1 and No. 2 were observed with a high power microscope and measured.
(10) Comparing the penetration depth of the No. 1 and No. 2 test skins, the skin penetration effect of the penetrated skins is good.
(11) And (5) sorting the test data and the pictures, and outputting a test report.
Referring to fig. 2, it can be seen from fig. 2 that the penetration depth of allura red for the skin section tested by the beauty instrument reached 0.4414mm, while the penetration depth of allura red for the skin section tested without the beauty instrument was only 0.1552 mm. Therefore, the beauty instrument can be judged to have good transdermal efficacy.
Example 2
The embodiment provides application of a method for verifying the transdermal efficacy of a cosmetic product in screening ultrasonic frequencies for the optimal transdermal efficacy of the cosmetic product.
Ultrasonic frequency verification flow chart for optimal transdermal efficacy as shown in fig. 3, the verification steps include:
(1) according to the number of frequency points (such as 20kHz, 30kHz, 50kHz and 1MHz) to be tested by the cosmetic product, the test skin to be tested is prepared, and each frequency point corresponds to one test skin.
(2) Each piece of skin was subjected to the corresponding frequency test using skin verification method No. 2 in example 1, and all the tests on the skin were completed in sequence.
(3) And sorting the data and the pictures obtained by testing each piece of skin, and sorting out a test document.
(4) The depth of penetration of all tested skins was compared to obtain the ultrasound frequency for optimal transdermal efficacy.
(5) The ultrasound frequency for optimum transdermal efficacy is applied to cosmetic products.
Example 3
The embodiment provides application of the verification method of the skin penetration efficacy of the cosmetic product in screening the optimal use time of the optimal skin penetration efficacy of the cosmetic product.
Best time to use verification flowchart for best transdermal efficacy as shown in fig. 4, the verification steps include:
(1) the number of test skins to be tested is determined according to the number of times of use of the cosmetic product to be tested, each time of use corresponding to one test skin, and the time points of use of the cosmetic product are generally 10 minutes, 15 minutes, 20 minutes, 25 minutes and 30 minutes.
(2) Each piece of skin was verified using skin verification method No. 2 in example 1 above, except that the time of use was varied, and all the skin tests were performed sequentially.
(3) And sorting the data and the pictures obtained by testing each piece of skin, and sorting out a test document.
(4) The depth of penetration of all tested skins was compared to give the time of use for the best transdermal effect.
(5) The application time for optimum transdermal efficacy is applied to cosmetic products.
Example 4
The embodiment provides application of a method for verifying the transdermal efficacy of a cosmetic product in screening the optimal temperature for the optimal transdermal efficacy of the cosmetic product.
Optimal temperature verification flow chart for optimal transdermal efficacy as shown in fig. 5, the verification steps include:
(1) test skins to be tested are prepared, each temperature point corresponding to a test skin, and the temperatures are generally selected to be 25 ℃, 37 ℃ and 40 ℃.
(2) The skin test method No. 2 in the embodiment 1 is matched with the corresponding temperature test for each piece of skin, and the test of all the tested skins is completed in sequence.
(3) And sorting the data and the pictures obtained by testing each piece of skin, and sorting out a test document.
(4) The depth of penetration of all tested skins was compared to obtain the temperature for optimal transdermal efficacy.
(5) The temperature for optimal transdermal efficacy is applied to the cosmetic product.
Example 5
The embodiment provides application of a method for verifying the transdermal efficacy of a cosmetic product in screening the optimal electric field intensity of the optimal transdermal efficacy of the cosmetic product.
Best electric field strength verification flowchart for best transdermal efficacy referring to fig. 6, the verification steps include:
(1) test skins to be tested are prepared, one for each field strength.
(2) The corresponding electric field strength test is carried out on each piece of skin by using the skin test method No. 2 in the embodiment 1, and the test of all the tested skins is completed in sequence.
(3) And sorting the data and the pictures obtained by testing each piece of skin, and sorting out a test document.
(4) The penetration depth of all tested skins was compared to obtain the electric field intensity for optimal transdermal efficacy.
(5) The electric field intensity for optimum transdermal efficacy is applied to cosmetic products.
Example 6
(1) Mixing glycerol (skin care product) and copper hydroxide (color developing agent) at a volume ratio of 100:1, and stirring to obtain purple mixed solution.
(2) Preparing test skin to be tested, smearing 0.1mL of the mixed solution prepared in the step (1) on one skin surface, and then placing for 30 minutes at 25 ℃; applying 0.1mL of the mixed solution prepared in step (1) to another piece of skin, allowing the solution to act on the skin for 10 minutes with an ion beauty instrument, and standing the skin at 25 ℃ for 20 minutes.
(3) And sorting the data and the pictures obtained by testing each piece of skin, and sorting out a test document.
(4) The depth of penetration of the test skin was compared and the depth of the purple color was examined.
Comparative example
The existing American Japanese skin penetration efficacy verification method comprises the following steps (shown in reference to FIG. 8):
(1) two test skins with the same part and the same size are selected, one skin is used for 15 minutes by a beauty instrument, and the other skin is not treated.
(2) The two skins were placed on two test tools, respectively, as shown in fig. 7.
(3) And after the test fixture and the conditions are prepared, the test fixture is operated.
(4) The content of the essence in the normal saline is detected at intervals (such as 1 hour), and the normal saline with equal volume is supplemented.
(5) Each test skin was tested 5 times and the data was collated.
(6) The skin penetration effect of the two pieces of skin is judged by comparing the detection data.
Essence supply port (donor chamber) in fig. 7: essence is placed on the part to provide penetrating essence for skin.
Test skin (membrane): can be made of human skin, pig skin or artificial skin.
Essence receiving end (receiver chamber): the inside of the box is filled with physiological saline simulating human body fluid for receiving essence.
Hot air inlet & outlet: is used for maintaining the temperature of the physiological saline at the essence receiving end and keeping the temperature of the physiological saline at about 37 ℃ of the human body.
Stirring apparatus (stir bar): the solution used for stirring the essence receiving end enables the distribution of the essence in the solution to be balanced, and subsequent sampling is facilitated.
Sampling port (Sampling port): the content of the essence in the normal saline is detected at intervals (such as 1 hour), and the normal saline with equal volume is supplemented.
The verification method for the skin penetration effect of the beauty product provided by the invention greatly simplifies the test process, not only can quickly see the test effect, but also can directly show the skin penetration effect to customers. The verification method does not need expensive test equipment, and reduces the test cost. In addition, the verification method simulates the actual use condition of a user, so that a customer can more easily understand the effect of the beauty product. The verification method provided by the invention can improve the function and performance of the beauty product and improve the transdermal efficacy, thereby promoting the development of the beauty product with better transdermal effect.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A method for verifying the transdermal efficacy of a cosmetic product is characterized by comprising the following steps: treating the test skin coated with the color-developing agent with a cosmetic product, then slicing the test skin, and obtaining the transdermal efficacy of the cosmetic product according to the penetration depth of the color-developing agent of the test skin under a microscope;
the test skin is pig skin or artificial skin.
2. The verification method according to claim 1, wherein the cosmetic product is an ultrasonic cosmetic product, the ultrasonic cosmetic product being at least one of: roller-type beauty treatment instrument, ultrasonic beauty treatment instrument, ion leading-out and leading-in beauty treatment instrument, steam-type beauty treatment instrument and LED beauty treatment instrument.
3. The validation method according to claim 1, wherein the color developer is allura red, carmine, amaranth, sunset yellow, erythrosine, tartrazine, indigo, or brilliant blue.
4. The verification method according to claim 1, comprising coating the test skin with the developer, and then treating the test skin coated with the developer with the cosmetic product, and repeating the coating and cosmetic product treating steps 2-10 times in this order;
preferably, the test skin is pig ear.
5. The verification method according to claim 4, further comprising placing the test skin after the last cosmetic product treatment in a set temperature environment, then washing the test skin, slicing the test skin to observe and measure the penetration depth;
preferably, the set temperature environment is placed for 20-30 minutes, and the set environment temperature is 37 +/-0.5 ℃.
6. The verification method according to claim 4, further comprising performing a control test for judging the transdermal efficacy of the cosmetic product by combining the results of the control test, wherein the control test comprises directly slicing the test skin coated with the color developer, and performing microscopic examination.
7. The validation method according to claim 6, wherein the control test comprises coating the test skin with the developer, then leaving the test skin coated with the developer at rest, repeating the coating and leaving steps 2-10 times in sequence, and then performing microscopic observation.
8. The verification method according to claim 1, wherein the verification method comprises coating the test skin with the beauty skin care product dissolved with the color developer, treating the test skin coated with the color developer and the beauty skin care product with the beauty product, slicing the test skin, and obtaining the transdermal efficacy of the beauty product according to the color developer penetration depth of the test skin under a microscope.
9. Use of the method of any one of claims 1-8 for screening cosmetic products for optimal transdermal efficacy of electric field intensity, optimal temperature, optimal ultrasound frequency, and optimal time of use.
10. The use according to claim 9, wherein said screening for optimal ultrasound frequencies for optimal transdermal efficacy of cosmetic products comprises: the number of the test skins is correspondingly set according to the number of the frequency points of the beauty products, each test skin coated with the color developing agent is matched with the corresponding frequency to carry out the treatment of the beauty products, then the test skin is sliced, and the transdermal efficacy of the beauty products is obtained under a microscope according to the penetration depth of the color developing agent of the test skin.
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| JP2013059556A (en) * | 2011-09-14 | 2013-04-04 | Hitachi Maxell Ltd | Beauty apparatus |
| CN105658200A (en) * | 2013-09-27 | 2016-06-08 | Ep科技有限公司 | Ethods and apparatus for delivery of molecules across layers of tissue |
| CN107441628A (en) * | 2010-08-27 | 2017-12-08 | 塞安纳生物制药公司 | For targetting the composition and method of thermal conditioning |
| WO2019049150A1 (en) * | 2017-09-07 | 2019-03-14 | Technion Research & Development Foundation Limited | Topical kits and compositions and use thereof |
| JP2020033335A (en) * | 2018-08-23 | 2020-03-05 | ポーラ化成工業株式会社 | Vesicle-containing composition |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107441628A (en) * | 2010-08-27 | 2017-12-08 | 塞安纳生物制药公司 | For targetting the composition and method of thermal conditioning |
| JP2013059556A (en) * | 2011-09-14 | 2013-04-04 | Hitachi Maxell Ltd | Beauty apparatus |
| CN105658200A (en) * | 2013-09-27 | 2016-06-08 | Ep科技有限公司 | Ethods and apparatus for delivery of molecules across layers of tissue |
| WO2019049150A1 (en) * | 2017-09-07 | 2019-03-14 | Technion Research & Development Foundation Limited | Topical kits and compositions and use thereof |
| JP2020033335A (en) * | 2018-08-23 | 2020-03-05 | ポーラ化成工業株式会社 | Vesicle-containing composition |
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