CN112646749A - Method for producing aureomycin seeds by adjusting pH of culture medium with ammonia water - Google Patents
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- 239000001963 growth medium Substances 0.000 title claims abstract description 33
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 title claims abstract description 21
- 235000011114 ammonium hydroxide Nutrition 0.000 title claims abstract description 21
- CYDMQBQPVICBEU-UHFFFAOYSA-N chlorotetracycline Natural products C1=CC(Cl)=C2C(O)(C)C3CC4C(N(C)C)C(O)=C(C(N)=O)C(=O)C4(O)C(O)=C3C(=O)C2=C1O CYDMQBQPVICBEU-UHFFFAOYSA-N 0.000 title claims abstract description 20
- 229960004475 chlortetracycline Drugs 0.000 title claims abstract description 20
- CYDMQBQPVICBEU-XRNKAMNCSA-N chlortetracycline Chemical compound C1=CC(Cl)=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O CYDMQBQPVICBEU-XRNKAMNCSA-N 0.000 title claims abstract description 20
- 235000019365 chlortetracycline Nutrition 0.000 title claims abstract description 20
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 20
- 238000000855 fermentation Methods 0.000 claims abstract description 37
- 230000004151 fermentation Effects 0.000 claims abstract description 37
- 241000186984 Kitasatospora aureofaciens Species 0.000 claims abstract description 5
- 238000002360 preparation method Methods 0.000 claims description 27
- 239000000843 powder Substances 0.000 claims description 15
- 239000002689 soil Substances 0.000 claims description 11
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 238000011218 seed culture Methods 0.000 claims description 10
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 6
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 5
- 244000105624 Arachis hypogaea Species 0.000 claims description 5
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 5
- 235000018262 Arachis monticola Nutrition 0.000 claims description 5
- 229920002261 Corn starch Polymers 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 5
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 5
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 5
- 239000008120 corn starch Substances 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- 235000020232 peanut Nutrition 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 239000000243 solution Substances 0.000 claims description 5
- 244000068988 Glycine max Species 0.000 claims description 4
- 235000010469 Glycine max Nutrition 0.000 claims description 4
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 4
- 239000012736 aqueous medium Substances 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims description 2
- 239000004099 Chlortetracycline Substances 0.000 claims 2
- 229910021529 ammonia Inorganic materials 0.000 claims 2
- 239000002609 medium Substances 0.000 claims 2
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 244000005700 microbiome Species 0.000 description 9
- 238000012360 testing method Methods 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 230000003139 buffering effect Effects 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 230000003204 osmotic effect Effects 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 239000000645 desinfectant Substances 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000007873 sieving Methods 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 244000000231 Sesamum indicum Species 0.000 description 1
- 235000003434 Sesamum indicum Nutrition 0.000 description 1
- 241000519995 Stachys sylvatica Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000009933 burial Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 239000002274 desiccant Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000011177 media preparation Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P29/00—Preparation of compounds containing a naphthacene ring system, e.g. tetracycline
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical field of aureomycin fermentation, and particularly discloses a method for producing aureomycin seeds by using ammonia water to adjust the pH of a culture medium, which uses streptomyces aureofaciens as a starting strain, and generates aureomycin through pure culture and three-stage fermentation.
Description
Technical Field
The invention belongs to the technical field of aureomycin fermentation, and particularly relates to a method for producing aureomycin seeds by using ammonia water to adjust the pH value of a culture medium.
Background
The fermentation production method of aureomycin uses streptomyces aureofaciens as an initial strain, and aureomycin is generated through pure culture and secondary fermentation, the normal growth of microorganism needs to be maintained under a certain pH value in the fermentation process, and the pH value has great influence on the growth of the microorganism and the generation of metabolites. The requirements of different microorganisms on the pH value are different, each microorganism has the pH value which is optimal and tolerant for growth, and the optimal pH value of most bacteria is 6.5-7.5; the most suitable pH value of the mould is 4.0-5.8, and the most suitable pH value of the microzyme is 3.8-6.0; the optimum pH value of the actinomycetes is 6.5-8.0, and the optimum pH range of some microorganisms in the growth and reproduction stage is inconsistent with that of the product generation stage, which is not only related to the characteristics of strains, but also related to the chemical properties of the product. Particularly in the fermentation production process of aureomycin, the pH value of the seed culture medium after consumption not only affects the germination of spores, but also affects the growth trend of seeds, and particularly affects the yield of antibiotics. The pH of the culture medium of the existing seeds is generally adjusted by using sodium hydroxide, but the sodium hydroxide belongs to strong base, damages nutrient substances of the culture medium, has weak buffering capacity on the pH, and simultaneously, sodium ions increase osmotic pressure of a microorganism growth environment to cause certain influence on the growth of the seeds.
Disclosure of Invention
The invention aims to provide a method for producing aureomycin seeds by using ammonia water to adjust the pH value of a culture medium.
In order to achieve the purpose, the invention adopts the technical scheme that:
a method for producing aureomycin seeds by using ammonia water to adjust the pH of a culture medium comprises the steps of taking streptomyces aureofaciens as a starting strain, carrying out pure culture and secondary fermentation to generate aureomycin, and specifically comprises the steps of a sandy soil spore preparation process, a mother bottle inclined surface spore preparation process, a son bottle inclined surface spore preparation process, a first-level seed preparation process and a fermentation preparation process, wherein the pH value of a seed culture medium in the first-level seed preparation process is adjusted to 6.15 +/-0.05 by ammonia water with the concentration of 22.5 percent, the addition amount of the ammonia water is 0.1 percent of the volume of the seed culture medium, and the osmotic pressure balance of a microorganism growth environment can be promoted and the rapid absorption of a nitrogen source by spores can be promoted due to the buffering and adjustment of the pH microenvironment of the ammonia water on the microorganism, 4L of the ammonia water is added, the concentration is 22.5 percent, the volume of the first-level seed solution is 4000 liters, in the seed preparation process, each liter of culture medium aqueous solution in the seed tank contains 20g of peanut cake powder, 10g of soybean cake powder, 35g of corn starch, 1.5g of sodium chloride, 2.5g of ammonium sulfate, 4g of calcium carbonate, 15g of yeast powder and 0.5g of monopotassium phosphate.
Furthermore, the fermentation production adopts variable temperature control, the culture temperature is 28-34 ℃ after inoculation, the temperature is reduced to 29 +/-3 ℃ after the mycelium grows well, the culture pressure is 0.01-0.04 MPa, the fermentation culture viscosity is 1-35 seconds, and the fermentation period is 90-124 hours.
Further, in the fermentation preparation process, the culture medium aqueous solution in each seed tank was 4000 liters.
The invention has the advantages that: the pH of the seed culture medium is adjusted by ammonia water with the concentration of 22.5 percent, on one hand, the pH can be buffered and adjusted to be 6.15 +/-0.05, which is the optimal pH range for the growth of the aureomycin seeds and promotes the osmotic pressure balance of the growth environment of microorganisms, on the other hand, a quick-acting nitrogen source can be provided for the growth of spores, the nitrogen source is quickly utilized in a better microenvironment to promote the seeds to quickly enter a logarithmic phase and to be transferred into a secondary fermentation tank in advance, the time is saved, and the quality and the price are low.
Detailed Description
Examples
A method for producing aureomycin seeds by using ammonia water to adjust the pH value of a culture medium comprises the following steps of taking streptomyces aureofaciens as a starting strain, carrying out pure culture and secondary fermentation to generate aureomycin, and specifically comprises the following process steps of sandy soil spore preparation, mother bottle inclined surface spore preparation, son bottle inclined surface spore preparation, primary seed preparation and fermentation preparation, wherein the pH value of the seed culture medium in the primary seed preparation is adjusted by the ammonia water, and the method specifically comprises the following steps:
(1) sandy soil spore preparation process: washing river sand with 10% hydrochloric acid soaking water, drying, sieving with a 60-mesh sieve, soaking soil with water, drying, sieving with a 80-mesh sieve, and mixing the sand and the soil according to the weight ratio of 2: 1, uniformly mixing in proportion, subpackaging in small test tubes of 12 x 100mm, the loading amount is about 1cm, sealing by using a gauze cotton plug, sterilizing at 126 ℃ for three times in an intermittent way for 1 hour, carrying out drying sterility test inspection, carrying out sterility test for later use, inoculating high-cost-effective oblique spores subjected to separation and screening into a checked sterile sandy soil tube under the aseptic condition, attaching a label, recording the name and the burial date of sandy soil, placing the tube in a container with a drying agent, covering and sealing, and placing the tube in a refrigerator at 0-10 ℃ for later use;
(2) the preparation process of the spores on the inclined surface of the mother bottle comprises the following steps: selecting a proper amount of sandy soil from a sandy soil pipe under the aseptic condition, connecting the sandy soil pipe with a mother bottle slant culture medium, requiring that the bacterial colony distribution is slightly dilute, after marking, placing the mixture in a thermostatic chamber with the temperature of 32 +/-1 ℃ and the humidity of 40-65% for culturing for 3-5 days, and placing the mixture in a refrigerator with the temperature of 0-10 ℃ for later use after the mixture is mature;
(3) the preparation process of the spore on the inclined surface of the seed bottle comprises the following steps: selecting 4-10 single colonies with plump spores and no white spots from a mother bottle, uniformly stirring in about 5ml of sterile water, selecting a spore solution by using a glass shovel, inoculating the spore solution onto a slant culture medium of a son bottle, requiring the dense distribution of the colonies, simultaneously making a dew dish and broth without test examination, identifying the inoculated slant, culturing in a thermostatic chamber with the temperature of 32 +/-1 ℃ and the humidity of 40-65% for 3-5 days, and storing in a refrigerator at the temperature of 0-10 ℃ for later use after maturation;
(4) a first-stage seed preparation procedure: in the seed culture medium preparation procedure, each liter of culture medium aqueous solution in a seed tank contains: 20g of peanut cake powder, 10g of soybean cake powder, 35g of corn starch, 1.5g of sodium chloride, 2.5g of ammonium sulfate, 4g of calcium carbonate, 15g of yeast powder and 0.5g of potassium dihydrogen phosphate, wherein the volume of the culture medium aqueous solution in each seed tank can be 4000 liters or 4m3Calculating, under the aseptic condition, adding 150ml of aseptic distilled water into qualified 4-6 bottle inclined plane spores, inoculating the spores into a first-level seed tank by a flame method, controlling the temperature at 26-33 ℃, the tank pressure at 0.04-0.06 Mpa, and the stirring speed at 110-140rpm for culturing for 20-26 hours, and the second-level seed liquid for 20-26 hours, wherein the seeds with good growth can be transferred into a fermentation tank；
(5) A fermentation preparation process: the fermentation tank contains per liter of culture medium water solution: 20g of peanut cake powder, 10g of soybean cake powder, 35g of corn starch, 1.5g of sodium chloride, 2.5g of ammonium sulfate, 4g of calcium carbonate, 15g of yeast powder and 0.5g of potassium dihydrogen phosphate, wherein the volume of the culture medium aqueous solution in each seed tank can be 4000 liters or 4m3The fermenter contained, calculated as a 30000 liter of aqueous medium: 600Kg of peanut cake powder, 300 Kg of sesame cake powder, 1800 Kg of corn starch, 3 Kg of amylase, 60 Kg of sodium chloride, 220 Kg of ammonium sulfate, 170 Kg of calcium carbonate, 260 Kg of yeast powder, 400 Kg of corn steep liquor, 6.6 Kg of magnesium sulfate, 3 Kg of potassium dihydrogen phosphate and 5 Kg of soybean oil, when the sterilization of basic culture medium is completed and the temperature is reduced to 30-35 ℃, the cultured secondary seeds are transferred into a fermentation tank, the temperature change control is adopted in the fermentation production after the transfer, the culture pressure is controlled to be 0.01-0.04 MPa, the fermentation culture viscosity is controlled to be 1-35 seconds, the fermentation period is 90-124 hours, and the culture medium is sent to an extraction workshop for extraction after the fermentation is completed.
Examples of the experiments
In order to reduce the production cost and improve the efficiency, the invention uses ammonia water with relatively low price to completely replace sodium hydroxide as a raw material, adjusts the pH value of a seed culture medium after the elimination, carries out a process improvement test, transfers the cultured first-grade seed into a fermentation culture medium, then carries out the culture, the pH adjustment of the seed culture medium in the test selects to replace the sodium hydroxide by the ammonia water, transfers the good seed into the fermentation culture medium, the fermentation is better, and the specific seed transplanting index and the fermentation productivity of the test are shown in tables 1 and 2:
TABLE 1 seed transplanting index
And (3) knotting: because ammonia water has a buffering effect, the pH fluctuation of the aqueous ammonia disinfectant is small and the aqueous ammonia disinfectant is more stable; in the aspect of seed transplanting time, compared with the original production, the invention shortens 1 hour; in the aspect of seed transferring pH, the invention is slightly lower than the original invention by 0.04 on average; the concentration of the bacteria is improved by 7 percent, which shows that the hypha grows faster and the seeds grow well, and lays a foundation for better subsequent fermentation performance.
TABLE 2 fermentation Capacity improvement
| Project (yield) | 15%CTC（kg） |
| Mean of the invention (20 batches) | 8586±48 |
| Average original production (20 batches) | 8256±99 |
And (3) knotting: because the seeds grow well and the subsequent fermentation is more stable, the yield of the method is improved by 330kg compared with the original production, the production fluctuation is small, and the effect is obvious.
Claims (3)
1. A method for producing aureomycin seeds by using ammonia water to adjust the pH of a culture medium comprises the following steps of taking streptomyces aureofaciens as a starting strain, carrying out pure culture and secondary fermentation to generate aureomycin, and specifically comprises the following process steps of sandy soil spore preparation, mother bottle inclined surface spore preparation, son bottle inclined surface spore preparation, primary seed preparation and fermentation preparation, and is characterized in that: in the first-stage seed preparation procedure, the pH value of a seed culture medium is adjusted to 6.15 +/-0.05 by ammonia water with the concentration of 22.5%, the addition amount of the ammonia water is 0.1% of the volume of the seed culture medium, and in the seed preparation procedure, each liter of culture medium aqueous solution in a seed tank contains 20g of peanut cake powder, 10g of soybean cake powder, 35g of corn starch, 1.5g of sodium chloride, 2.5g of ammonium sulfate, 4g of calcium carbonate, 15g of yeast powder and 0.5g of potassium dihydrogen phosphate.
2. The method for producing a chlortetracycline seed according to claim 1 using ammonia to adjust the pH of the medium, wherein: the fermentation production adopts variable temperature control, the culture temperature after inoculation is 28-34 ℃, the temperature is reduced to 29 +/-3 ℃ after the mycelium grows well, the culture pressure is 0.01-0.04 Mpa, the viscosity of the fermentation culture is 1-35 seconds, and the fermentation period is 90-124 hours.
3. The method for producing a chlortetracycline seed according to claim 2 using ammonia to adjust the pH of the medium, wherein: in the fermentation preparation process, the aqueous medium solution in each seed tank was 4000 liters.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103614445A (en) * | 2013-03-29 | 2014-03-05 | 驻马店华中正大有限公司 | A fermentation production method for aureomycin by utilizing mycoprotein in place of a portion of yeast powder |
| WO2018120574A1 (en) * | 2016-12-30 | 2018-07-05 | 内蒙古金达威药业有限公司 | Method for producing dha by microbial fermentation |
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2021
- 2021-01-15 CN CN202110057019.XA patent/CN112646749A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103614445A (en) * | 2013-03-29 | 2014-03-05 | 驻马店华中正大有限公司 | A fermentation production method for aureomycin by utilizing mycoprotein in place of a portion of yeast powder |
| WO2018120574A1 (en) * | 2016-12-30 | 2018-07-05 | 内蒙古金达威药业有限公司 | Method for producing dha by microbial fermentation |
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