CN112626146A - Preparation method of high-purity stachyose - Google Patents
Preparation method of high-purity stachyose Download PDFInfo
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- CN112626146A CN112626146A CN202011559381.9A CN202011559381A CN112626146A CN 112626146 A CN112626146 A CN 112626146A CN 202011559381 A CN202011559381 A CN 202011559381A CN 112626146 A CN112626146 A CN 112626146A
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- stachyose
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
- C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
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Abstract
The invention discloses a preparation method of high-purity stachyose, which comprises the following steps: s1, washing and baking the raw materials to obtain dried raw materials; s2, leaching and juicing the raw material water, and filtering to obtain a sugar solution; s3, adding yeast into the sugar solution, standing and culturing at constant temperature to obtain fermentation liquor; s4, adding calcium hydroxide serving as a clarifying agent into the fermentation liquor, and taking supernate; s5, adding activated carbon into the supernatant, and taking the filtrate; s6, introducing the filtrate into a nanofiltration membrane for purification to obtain a purified solution; s7, introducing the purified solution into ion exchange resin to obtain stachyose solution; s8, concentrating and drying the stachyose solution to obtain the stachyose. The method has the advantages of simple steps, wide material sources, convenient operation, complete technology, easy process control, convenience for large-scale industrial production and creation of higher social benefits. The experimental result shows that the prepared stachyose has high purity and has obvious competitive advantage compared with the similar products.
Description
Technical Field
The invention relates to the technical field related to saccharide production, in particular to a preparation method of high-purity stachyose.
Background
Stachyose is a naturally occurring tetrasaccharide, and can significantly promote the proliferation of beneficial bacteria such as bifidobacterium and the like. The pure product is white powder, has slightly sweet taste, sweetness of 22 percent of that of the cane sugar, pure taste and no bad taste or peculiar smell. The molecular structure is as follows: "galactose-glucose-fructose". It can promote the formation of dominant bacteria status of beneficial bacteria in digestive tract, inhibit the production of putrefying bacteria such as Clostridium acidogenum and the like, produce a large amount of physiological active substances, regulate the pH value of intestinal tract, kill pathogenic bacteria, inhibit the generation of putrefying products, inhibit the generation and absorption of endogenous carcinogens, and decompose and derive multiple immune function factors. However, the purity of the stachyose product obtained by the existing preparation method is not ideal enough, and the purity of the prepared stachyose is not high.
Disclosure of Invention
In order to solve the above-mentioned defects, the present invention aims to provide a method for preparing high-purity stachyose, and the prepared stachyose has the characteristic of high purity.
In order to achieve the purpose, the invention is realized by the following technical scheme: a preparation method of high-purity stachyose comprises the following steps:
s1, placing the raw materials into a cleaning device for cleaning, transferring the cleaned raw materials into a baking device for baking to obtain dried raw materials;
s2, adding water with the temperature of 70-80 ℃ into the raw materials, extracting juice, and filtering to obtain sugar solution;
s3, adding yeast into the sugar solution, performing static culture at a constant temperature of 20-30 ℃ for 20-40h, performing static culture at a constant temperature of 35-42 ℃ for 18-23h, and terminating fermentation to obtain fermentation liquor;
s4, adding calcium hydroxide as a clarifying agent into the fermentation liquor according to the ratio of 8-15g to 1L, and taking supernatant;
s5, adding activated carbon into the supernatant, heating to 70-80 ℃, decoloring for 20-50min, filtering to remove the activated carbon, and taking the filtrate;
s6, introducing the filtrate into a nanofiltration membrane for purification to obtain a purified solution;
s7, introducing the purified solution into ion exchange resin to obtain stachyose solution;
s8, concentrating and drying the stachyose solution to obtain the stachyose.
Further, in step S1, the raw material is selected from one or more of cordyceps sinensis, spirulina, pagoda and momordica grosvenori.
Further, in step S1, the cleaning device is a high-pressure washing device with an ultrasonic cleaning device installed therein.
Further, in the step S2, a cloth bag is adopted during filteringFiltering with a cloth bag filtering area of 1-10m2The filtering aperture is 1-100um, the filtering pressure is 0.1-0.5MPa, and the water flow is 50-500T/h.
Further, in the step S3, the amount of yeast added is 0.5 to 1% by mass of the sugar solution.
The invention has the beneficial effects that: the method has the advantages of simple steps, wide material sources, convenient operation, complete technology, easy process control, convenience for large-scale industrial production and creation of higher social benefits. The experimental result shows that the prepared stachyose has high purity and has obvious competitive advantage compared with the similar products.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
A preparation method of high-purity stachyose comprises the following steps:
s1, placing the cordyceps sinensis and the plectranthus chinensis into a high-pressure washing device provided with an ultrasonic cleaning device for cleaning, and transferring the cleaned raw materials into a baking device for baking to obtain dried raw materials;
s2, adding 70 deg.C water into the raw materials, extracting, squeezing, and filtering with cloth bag with filtering area of 1m2Filtering with the aperture of 1um, the filtering pressure of 0.1MPa and the water flow rate of 50T/h to obtain sugar liquid;
s3, adding yeast into the sugar solution, wherein the adding amount of the yeast is 0.5 percent of the mass of the sugar solution, performing static culture at a constant temperature of 20 ℃ for 20 hours, performing static culture at a constant temperature of 35 ℃ for 18 hours, and stopping fermentation to obtain fermentation liquor;
s4, adding calcium hydroxide serving as a clarifying agent into the fermentation liquor according to the ratio of 8g to 1L, and taking supernatant;
s5, adding activated carbon into the supernatant, heating to 70 ℃, decoloring for 20min, filtering to remove the activated carbon, and taking the filtrate;
s6, introducing the filtrate into a nanofiltration membrane for purification to obtain a purified solution;
s7, introducing the purified solution into ion exchange resin to obtain stachyose solution;
s8, concentrating and drying the stachyose solution to obtain the stachyose with the purity of 88%.
Example 2
A preparation method of high-purity stachyose comprises the following steps:
s1, placing the cordyceps sinensis and the plectranthus chinensis into a high-pressure washing device provided with an ultrasonic cleaning device for cleaning, and transferring the cleaned raw materials into a baking device for baking to obtain dried raw materials;
s2, adding 75 deg.C water into the raw materials, extracting, squeezing, and filtering with cloth bag with filtering area of 5m2Filtering with the aperture of 50um, the filtering pressure of 0.3MPa and the water flow rate of 300T/h to obtain sugar liquid;
s3, adding yeast into the sugar solution, wherein the adding amount of the yeast is 0.8 percent of the mass of the sugar solution, performing static culture at a constant temperature of 25 ℃ for 30 hours, performing static culture at a constant temperature of 39 ℃ for 20 hours, and stopping fermentation to obtain fermentation liquor;
s4, adding calcium hydroxide serving as a clarifying agent into the fermentation liquor according to the proportion of 12g to 1L, and taking supernate;
s5, adding activated carbon into the supernatant, heating to 75 ℃, decoloring for 35min, filtering to remove the activated carbon, and taking the filtrate;
s6, introducing the filtrate into a nanofiltration membrane for purification to obtain a purified solution;
s7, introducing the purified solution into ion exchange resin to obtain stachyose solution;
s8, concentrating and drying the stachyose solution to obtain the stachyose with the purity of 90%.
Example 3
A preparation method of high-purity stachyose comprises the following steps:
s1, placing the cordyceps sinensis and the plectranthus chinensis into a high-pressure washing device provided with an ultrasonic cleaning device for cleaning, and transferring the cleaned raw materials into a baking device for baking to obtain dried raw materials;
s2, adding water of 80 ℃ into the raw materials, extracting and juicing, filtering by a cloth bag, wherein the filtering area of the cloth bag is 10m2Filtering with aperture of 100um, filtering pressure of 0.5MPa and water flow rate of 500T/h to obtain sugar solution;
s3, adding yeast into the sugar solution, wherein the adding amount of the yeast is 1% of the mass of the sugar solution, performing constant-temperature static culture at 30 ℃ for 40h, performing constant-temperature static culture at 42 ℃ for 23h, and stopping fermentation to obtain fermentation liquor;
s4, adding calcium hydroxide serving as a clarifying agent into the fermentation liquor according to the proportion of 15g to 1L, and taking supernate;
s5, adding activated carbon into the supernatant, heating to 80 ℃, decoloring for 50min, filtering to remove the activated carbon, and taking the filtrate;
s6, introducing the filtrate into a nanofiltration membrane for purification to obtain a purified solution;
s7, introducing the purified solution into ion exchange resin to obtain stachyose solution;
s8, concentrating and drying the stachyose solution to obtain the stachyose with the purity of 93%.
In conclusion, the method has the advantages of simple steps, wide material sources, convenient operation, complete technology, easy process control, convenience for large-scale industrial production and creation of higher social benefits. The experimental result shows that the prepared stachyose has high purity and has obvious competitive advantage compared with the similar products.
The embodiments of the present invention have been described in detail, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in the embodiments without departing from the principles and spirit of the invention, and these embodiments are within the scope of the invention.
Claims (5)
1. The preparation method of the high-purity stachyose is characterized by comprising the following steps:
s1, placing the raw materials into a cleaning device for cleaning, transferring the cleaned raw materials into a baking device for baking to obtain dried raw materials;
s2, adding water with the temperature of 70-80 ℃ into the raw materials, extracting juice, and filtering to obtain sugar solution;
s3, adding yeast into the sugar solution, performing static culture at a constant temperature of 20-30 ℃ for 20-40h, performing static culture at a constant temperature of 35-42 ℃ for 18-23h, and terminating fermentation to obtain fermentation liquor;
s4, adding calcium hydroxide as a clarifying agent into the fermentation liquor according to the ratio of 8-15g to 1L, and taking supernatant;
s5, adding activated carbon into the supernatant, heating to 70-80 ℃, decoloring for 20-50min, filtering to remove the activated carbon, and taking the filtrate;
s6, introducing the filtrate into a nanofiltration membrane for purification to obtain a purified solution;
s7, introducing the purified solution into ion exchange resin to obtain stachyose solution;
s8, concentrating and drying the stachyose solution to obtain the stachyose.
2. The method for preparing high purity stachyose according to claim 1, wherein: in the step S1, the raw materials are selected from one or more of Cordyceps sinensis, Scrophularia ningpoensis, Potentilla chinensis and Potentilla chinensis.
3. The method for preparing high purity stachyose according to claim 1, wherein: in step S1, the cleaning device is a high-pressure washing device equipped with an ultrasonic cleaning device.
4. The method for preparing high purity stachyose according to claim 1, wherein: in the step S2, a cloth bag is adopted for filtration, and the filtration area of the cloth bag is 1-10m2The filtering aperture is 1-100um, the filtering pressure is 0.1-0.5MPa, and the water flow is 50-500T/h.
5. The method for preparing high purity stachyose according to claim 1, wherein: in the step S3, the adding amount of the yeast is 0.5-1% of the mass of the sugar liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011559381.9A CN112626146A (en) | 2020-12-25 | 2020-12-25 | Preparation method of high-purity stachyose |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011559381.9A CN112626146A (en) | 2020-12-25 | 2020-12-25 | Preparation method of high-purity stachyose |
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| CN112626146A true CN112626146A (en) | 2021-04-09 |
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| CN202011559381.9A Withdrawn CN112626146A (en) | 2020-12-25 | 2020-12-25 | Preparation method of high-purity stachyose |
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2020
- 2020-12-25 CN CN202011559381.9A patent/CN112626146A/en not_active Withdrawn
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Application publication date: 20210409 |
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