CN112625898A - High-purity nucleic acid extraction device and working method thereof - Google Patents
High-purity nucleic acid extraction device and working method thereof Download PDFInfo
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- 238000000605 extraction Methods 0.000 title claims abstract description 79
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 50
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 50
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 8
- 230000000694 effects Effects 0.000 claims abstract description 14
- 238000012360 testing method Methods 0.000 claims description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 230000010355 oscillation Effects 0.000 claims description 9
- 125000004122 cyclic group Chemical group 0.000 claims description 6
- 238000005485 electric heating Methods 0.000 claims description 5
- 230000000149 penetrating effect Effects 0.000 claims description 4
- 239000007788 liquid Substances 0.000 abstract description 5
- 238000013461 design Methods 0.000 abstract description 3
- 230000007547 defect Effects 0.000 abstract description 2
- 238000009434 installation Methods 0.000 abstract description 2
- 238000003756 stirring Methods 0.000 description 5
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 102000011931 Nucleoproteins Human genes 0.000 description 1
- 108010061100 Nucleoproteins Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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Abstract
The invention discloses a high-purity nucleic acid extraction device and a working method thereof, and the high-purity nucleic acid extraction device comprises a nucleic acid extraction device body, a bracket and a central disc, wherein the bracket is arranged in the nucleic acid extraction device body, the central disc is arranged at the center of the bracket, an annular extraction plate is arranged on the upper end surface of the bracket through an installation groove, a plurality of clamping and oscillating devices are uniformly arranged in the annular extraction plate in a surrounding manner, a main support column is vertically arranged at the bottom of the central disc, auxiliary support rods are symmetrically and vertically arranged on two sides of the bottom of the bracket, bases are fixedly arranged at the bottoms of the main support column and the auxiliary support rods, and one end of the bracket is horizontally connected. The invention overcomes the defects of the prior art, has reasonable design, utilizes the horizontal movement of the bracket and the self-rotation of the central disc to ensure that the nucleic acid extraction device is continuously vibrated, so that the mixed liquid is quickly and uniformly mixed, the vibration efficiency and effect are improved, and the invention has higher social use value and application prospect.
Description
Technical Field
The invention relates to the technical field of nucleic acid extraction, in particular to a high-purity nucleic acid extraction device and a working method thereof.
Background
Biological macromolecular compounds, in which nucleic acids are polymerized from many nucleotides, are one of the most basic substances of life. Nucleic acid is widely present in all animal and plant cells and microorganisms, and nucleic acid in organisms is often combined with protein to form nucleoprotein. Different nucleic acids differ in their chemical composition, nucleotide arrangement order, and the like.
The current nucleic acid extraction reaction system is generally below 1ml, but in many experiments, free nucleic acid needs to be extracted from a large amount of samples, and experimental samples of large-volume reaction systems are needed. And the stirring of the mixed liquid of sample rocks the nucleic acid extraction appearance through the manual work usually and stirs the mixed liquid of sample, and manual stirring can increase the hand labor power, and the effect of manual stirring is relatively poor, and the efficiency of stirring is lower.
Therefore, the inventor provides a high-purity nucleic acid extraction device and a working method thereof in order to achieve the purpose of more practical value, with the experience of design development and actual manufacturing which is abundant in the related industry for many years, and research and improvement are performed on the existing structure and deficiency.
Disclosure of Invention
In order to solve the problems mentioned in the background art, the present invention provides a high purity nucleic acid extraction apparatus and a method for operating the same.
In order to achieve the purpose, the invention adopts the following technical scheme:
a high-purity nucleic acid extraction device comprises a nucleic acid extraction device body, a bracket and a central disc, wherein the bracket is arranged in the nucleic acid extraction device body, the central disc is arranged at the center of the bracket, an annular extraction plate is arranged on the upper end surface of the bracket through a mounting groove, a plurality of clamping and oscillating devices are uniformly arranged in the annular extraction plate in a surrounding manner, a main support column is vertically arranged at the bottom of the central disc, auxiliary support rods are symmetrically and vertically arranged at the two sides of the bottom of the bracket, bases are fixedly arranged at the bottoms of the main support column and the auxiliary support rods, one end of the bracket is horizontally connected with a first movable rod, a baffle is arranged at the end, penetrating through the side wall of the nucleic acid extraction device body, extending outwards, of the first movable rod, a second elastic part is sleeved on the outer wall of the first movable rod, the other end of the bracket is horizontally connected with a second movable rod, one end of the connecting rod, which is far away from the second movable rod, is rotatably connected with an eccentric shaft, the eccentric shaft is arranged at the eccentric position of the side wall of the circular disc, and the circular disc is connected with a first driving motor through a first rotating shaft.
Preferably, the centre gripping vibration device includes the clamping part of two symmetries, the inner wall of bracket is equipped with the thing groove of putting that corresponds with the centre gripping vibration device, and outer wall one side of clamping part is the concave arc face, and the lateral wall of concave arc face is equipped with the one deck slipmat, the clamping part is equipped with spacing branch for one side level of concave arc face, and spacing branch extends to the lateral wall of putting the thing groove, and the lateral wall of putting the thing groove is equipped with the spacing movable groove that corresponds with spacing branch, first elastic component has been cup jointed to the outer wall of spacing branch, the clamping part is connected with the connecting piece between the lateral wall of putting the thing groove.
Preferably, the connecting piece comprises four supporting rods, and adjacent supporting rods are movably connected through a pin shaft.
Preferably, the centre gripping has inserted in the vibration device and draws the test tube, the bottom of bracket is equipped with and is used for drawing the test tube to the water bath of usefulness.
Preferably, the circumferential side wall of the annular extraction plate is provided with a first annular bump, and the mounting groove is internally provided with a first annular groove corresponding to the first annular bump.
Preferably, the inner ring wall of the annular extraction plate is provided with a second annular bump, and the circumferential side wall of the central disk is provided with a second annular groove corresponding to the second annular bump.
Preferably, the inner ring wall of the annular extraction plate is provided with a ring of teeth, and the outer wall of the teeth is in meshing connection with a driving gear.
Preferably, the bottom of the base is equidistantly provided with a plurality of sliding blocks, the bottom of each sliding block is provided with a ball, and the inner bottom of the nucleic acid extraction device body is provided with a sliding groove corresponding to the sliding block.
Preferably, the driving gear is connected with a second driving motor through a second rotating shaft.
Preferably, the method comprises the following steps: vertically placing the extraction test tube in a clamping and vibrating device, and in order to fully realize the check extraction effect, arranging a water bath at the bottom of the extraction test tube, wherein the water bath heats the extraction test tube in an electric heating mode;
the first driving motor drives the circular disc to rotate through the first rotating shaft, the circular disc drives the eccentric shaft to rotate, and the eccentric shaft drives the second movable rod to horizontally reciprocate through the connecting rod, so that the horizontal oscillation of the bracket is realized;
the second driving motor drives the driving gear to rotate, and the driving gear drives the teeth of the inner ring of the annular extracting plate to rotate when rotating, so that the self-rotation of the annular extracting plate is realized, and the oscillation effect is enhanced.
Compared with the prior art, the invention has the beneficial effects that:
1. under the elastic support of the first elastic piece and the connecting piece, the effective clamping of the extraction test tube can be realized, and the clamping device can be suitable for clamping extraction test tubes with different sizes;
2. in order to fully realize the check extraction effect, a water bath is arranged at the bottom of the extraction test tube and heats the extraction test tube in an electric heating mode;
3. the first driving motor drives the circular disc to rotate through the first rotating shaft, the circular disc drives the eccentric shaft to rotate, the eccentric shaft drives the second movable rod to horizontally reciprocate through the connecting rod, the first movable rod can support the bracket at the moment, and meanwhile, the bracket horizontally vibrates under the action of the elastic force of the second elastic piece;
4. second driving motor drive driving gear rotates, and the tooth that drives cyclic annular extraction board inner circle when the driving gear rotates, and then realizes cyclic annular extraction board's rotation of autogyration to further strengthen vibrating the effect, help nucleic acid to draw.
In conclusion, the invention overcomes the defects of the prior art, has reasonable design, utilizes the horizontal motion of the bracket and the self-rotation of the central disc to ensure that the nucleic acid extraction device continuously vibrates, ensures that the mixed liquid is quickly and uniformly mixed, improves the vibration efficiency and effect, and has higher social use value and application prospect.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is an enlarged view of the structure A of the present invention;
FIG. 3 is a schematic structural view of the bracket of the present invention;
FIG. 4 is an enlarged view of the structure B of the present invention;
FIG. 5 is a perspective view of the annular extraction plate of the present invention;
FIG. 6 is a schematic cross-sectional view of a C-C structure according to the present invention.
In the figure: the nucleic acid extraction device comprises a nucleic acid extraction device body 1, a bracket 2, a central disc 3, an annular extraction plate 4, a first annular bump 41, a first annular groove 42, a second annular bump 43, teeth 44, a clamping oscillation device 5, a clamping part 51, a non-slip mat 52, a storage groove 53, a limiting support rod 54, a first elastic piece 55, a limiting movable groove 56, a connecting piece 57, an extraction test tube 6, a water bath 7, a main support column 8, an auxiliary support rod 9, a base 10, a sliding block 11, a ball 12, a sliding groove 13, a first movable rod 14, a second elastic piece 15, a baffle 16, a second movable rod 17, a connecting rod 18, an eccentric shaft 19, a circular disc 20, a first driving motor 21 and a driving gear 22.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to FIGS. 1 to 6, a high purity nucleic acid extraction device comprises a nucleic acid extraction device body 1, a bracket 2 and a central disc 3, wherein the bracket 2 is arranged in the nucleic acid extraction device body 1, the central disc 3 is arranged at the center of the bracket 2, an annular extraction plate 4 is arranged on the upper end surface of the bracket 2 through an installation groove, a plurality of clamping and oscillating devices 5 are uniformly arranged in the annular extraction plate 4 in a surrounding manner, a main support column 8 is vertically arranged at the bottom of the central disc 3, auxiliary support rods 9 are symmetrically and vertically arranged at two sides of the bottom of the bracket 2, a base 10 is fixedly arranged at the bottoms of the main support column 8 and the auxiliary support rods 9, a first movable rod 14 is horizontally connected with one end of the bracket 2, a baffle 16 is arranged at the outward extending end of the first movable rod 14 penetrating through the side wall of the nucleic acid extraction device body 1, a second elastic member 15 is sleeved on the outer wall of the first movable, the second movable rod 17 penetrates through the side wall of the nucleic acid extracting device body 1, the end extending outwards is rotatably connected with a connecting rod 18, one end of the connecting rod 18, which is far away from the second movable rod 17, is rotatably connected with an eccentric shaft 19, the eccentric shaft 19 is arranged at the eccentric position of the side wall of a circular disc 20, and the circular disc 20 is connected with a first driving motor 21 through a first rotating shaft.
Wherein, centre gripping oscillation device 5 includes the clamping part 51 of two symmetries, the inner wall of bracket 2 is equipped with puts the thing groove 53 with what centre gripping oscillation device 5 corresponded, outer wall one side of clamping part 51 is the concave arc face, the lateral wall of concave arc face is equipped with one deck slipmat 52, clamping part 51 is equipped with spacing branch 54 for one side level of concave arc face, spacing branch 54 extends to the lateral wall of putting thing groove 53, the lateral wall of putting thing groove 53 is equipped with the spacing movable groove 56 that corresponds with spacing branch 54, first elastic component 55 has been cup jointed to the outer wall of spacing branch 54, be connected with connecting piece 57 between clamping part 51 and the lateral wall of putting thing groove 53.
Wherein, connecting piece 57 comprises four branch poles, and through round pin axle swing joint between the adjacent branch pole, connecting piece 57 plays certain supporting role.
Wherein, the interpolation of centre gripping vibrating device 5 is connected with and draws test tube 6, and the bottom of bracket 2 is equipped with and is used for drawing test tube 6 to the water bath 7 of usefulness, heats through water bath 7 and draws test tube 6 to help nucleic acid to draw.
Wherein, the circumferential side wall of the annular extraction plate 4 is provided with a first annular bump 41, a first annular groove 42 corresponding to the first annular bump 41 is arranged in the mounting groove, and the first annular groove 42 carries out limiting support on the first annular bump 41.
Wherein, the inner ring wall of the annular extracting plate 4 is provided with a second annular bump 43, the circumferential side wall of the central disc 3 is provided with a second annular groove corresponding to the second annular bump 43, and the second annular groove carries out spacing support on the second annular bump 43.
Wherein, the inner circle wall of cyclic annular extraction plate 4 is equipped with a circle tooth 44, and the outer wall meshing of tooth 44 is connected with driving gear 22, and driving gear 22 is connected with second driving motor through the second pivot, and second driving motor drive driving gear 22 rotates, and driving gear 22 drives the tooth 44 rotation of cyclic annular extraction plate 4 inner circle when rotating, and then realizes cyclic annular extraction plate 4's self-rotation to strengthen vibrating the effect.
Wherein, the bottom of the base 10 is equidistantly provided with a plurality of slide blocks 11, the bottom of the slide block 11 is provided with a ball 12, the inner bottom of the nucleic acid extracting device body 1 is provided with a slide groove 13 corresponding to the slide block 11, the slide block 11 slides in the slide groove 13, and the slide block 11 is limited by the slide groove 13.
Wherein, the extraction test tube 6 is vertically placed in the clamping and oscillating device 5, in order to fully realize the check extraction effect, a water bath 7 is arranged at the bottom of the extraction test tube 6, and the water bath 7 heats the extraction test tube 6 in an electric heating mode;
the first driving motor 21 drives the circular disc 20 to rotate through the first rotating shaft, the circular disc 20 drives the eccentric shaft 19 to rotate, and the eccentric shaft 19 drives the second movable rod 17 to horizontally reciprocate through the connecting rod 18, so that the horizontal oscillation of the bracket 2 is realized.
The working principle is as follows: in the invention, when in use, the extraction test tube 6 filled with the sample mixed liquid is vertically placed in the clamping and oscillating device 5, the clamping parts 51 at two sides can clamp the extraction test tube 6, and have certain anti-skid effect under the action of the anti-skid pad 52, and meanwhile, under the elastic support of the first elastic piece 55 and the connecting piece 57, the effective clamping of the extraction test tube 6 can be realized, and the clamping device can be suitable for clamping extraction test tubes 6 with different sizes;
in order to fully realize the check extraction effect, a water bath 7 is arranged at the bottom of the extraction test tube 6, and the water bath 7 heats the extraction test tube 6 in an electric heating mode;
starting a first driving motor 21, wherein the first driving motor 21 drives a circular disc 20 to rotate through a first rotating shaft, the circular disc 20 drives an eccentric shaft 19 to rotate, the eccentric shaft 19 drives a second movable rod 17 to horizontally reciprocate through a connecting rod 18, and at the moment, the first movable rod 14 can play a supporting role for the bracket 2, and meanwhile, the bracket 2 horizontally vibrates under the action of elastic force of a second elastic piece 15;
meanwhile, the sliding block 11 at the bottom of the base 10 horizontally slides in the sliding groove 13, and the sliding block 11 is limited through the sliding groove 13, so that the bracket 2 can stably and horizontally vibrate;
and start the second driving motor, second driving motor drive driving gear 22 rotates, drives the tooth 44 rotation of annular extraction plate 4 inner circle when driving gear 22 rotates, and then realizes the rotation of annular extraction plate 4 to further strengthen and vibrate the effect, help nucleic acid to draw.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting of the invention.
In the present invention, unless otherwise expressly stated or limited, the terms "disposed," "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; they may be mechanically coupled, directly coupled, or indirectly coupled through intervening agents, both internally and/or in any other manner known to those skilled in the art. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (10)
1. A high-purity nucleic acid extraction device comprises a nucleic acid extraction device body (1), a bracket (2) and a central disk (3), wherein the bracket (2) is arranged in the nucleic acid extraction device body (1), and the central disk (3) is arranged at the center of the bracket (2), and is characterized in that: the upper end face of the bracket (2) is provided with an annular extraction plate (4) through a mounting groove, a plurality of clamping oscillation devices (5) are uniformly arranged in the annular extraction plate (4) in a surrounding manner, a main support column (8) is vertically arranged at the bottom of the central disc (3), auxiliary support rods (9) are symmetrically and vertically arranged at two sides of the bottom of the bracket (2), bases (10) are fixedly arranged at the bottoms of the main support column (8) and the auxiliary support rods (9), one end of the bracket (2) is horizontally connected with a first movable rod (14), a baffle (16) is arranged at the outward extending end of the first movable rod (14) penetrating through the side wall of the nucleic acid extraction device body (1), a second elastic part (15) is sleeved on the outer wall of the first movable rod (14), a second movable rod (17) is horizontally connected at the other end of the bracket (2), and the outward extending end of the second movable rod (17) penetrating through the side wall of the nucleic acid extraction, one end, far away from the second movable rod (17), of the connecting rod (18) is rotatably connected with an eccentric shaft (19), the eccentric shaft (19) is arranged at the eccentric position of the side wall of the circular disc (20), and the circular disc (20) is connected with a first driving motor (21) through a first rotating shaft.
2. The apparatus for extracting high purity nucleic acid according to claim 1, wherein: the utility model discloses a clamping vibration device, including the clamping part (51) of two symmetries, the inner wall of bracket (2) is equipped with puts thing groove (53) that corresponds with clamping vibration device (5), and outer wall one side of clamping part (51) is the concave arc face, and the lateral wall of concave arc face is equipped with one deck slipmat (52), clamping part (51) are equipped with spacing branch (54) for one side level of concave arc face, and spacing branch (54) extend to the lateral wall of putting thing groove (53), and the lateral wall of putting thing groove (53) is equipped with spacing movable groove (56) that correspond with spacing branch (54), first elastic component (55) have been cup jointed to the outer wall of spacing branch (54), clamping part (51) and put and be connected with connecting piece (57) between the lateral wall of thing groove (53).
3. The apparatus for extracting high purity nucleic acid according to claim 2, wherein: the connecting piece (57) is composed of four supporting rods, and the adjacent supporting rods are movably connected through pin shafts.
4. The apparatus for extracting high purity nucleic acid according to claim 2, wherein: the interpolation of centre gripping vibration device (5) has the test tube (6) of drawing, the bottom of bracket (2) is equipped with and is used for drawing test tube (6) water bath (7) to the usefulness.
5. The apparatus for extracting high purity nucleic acid according to claim 1, wherein: the circumference lateral wall of cyclic annular board (4) of drawing is equipped with first annular lug (41), be equipped with in the mounting groove with first annular lug (41) corresponding first annular groove (42).
6. The apparatus for extracting high purity nucleic acid according to claim 1, wherein: the inner ring wall of the annular extracting plate (4) is provided with a second annular bump (43), and the circumferential side wall of the central disc (3) is provided with a second annular groove corresponding to the second annular bump (43).
7. The apparatus for extracting high purity nucleic acid according to claim 6, wherein: the inner ring wall of the annular extraction plate (4) is provided with a ring of teeth (44), and the outer wall of the teeth (44) is engaged and connected with a driving gear (22).
8. The apparatus for extracting high purity nucleic acid according to claim 1, wherein: the bottom of the base (10) is equidistantly provided with a plurality of sliding blocks (11), the bottom of each sliding block (11) is provided with a ball (12), and the inner bottom of the nucleic acid extraction device body (1) is provided with a sliding groove (13) corresponding to each sliding block (11).
9. The apparatus for extracting high purity nucleic acid according to claim 7, wherein: the driving gear (22) is connected with a second driving motor through a second rotating shaft.
10. A working method of a high-purity nucleic acid extraction device is characterized by comprising the following steps: vertically placing an extraction test tube (6) in a clamping and vibrating device (5), and in order to fully realize the check extraction effect, arranging a water bath (7) at the bottom of the extraction test tube (6), wherein the water bath (7) heats the extraction test tube (6) in an electric heating mode;
the first driving motor (21) drives the circular disc (20) to rotate through the first rotating shaft, the circular disc (20) drives the eccentric shaft (19) to rotate, and the eccentric shaft (19) drives the second movable rod (17) to horizontally reciprocate through the connecting rod (18), so that the horizontal oscillation of the bracket (2) is realized;
the second driving motor drives the driving gear (22) to rotate, and the driving gear (22) drives the teeth (44) of the inner ring of the annular extracting plate (4) to rotate, so that the self-rotation of the annular extracting plate (4) is realized, and the oscillation effect is enhanced.
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| CN113528298A (en) * | 2021-07-16 | 2021-10-22 | 苏州江镨健康科技有限公司 | Nucleic acid extraction device and method for improving nucleic acid extraction precision |
| CN113621489A (en) * | 2021-08-10 | 2021-11-09 | 安徽惠恩生物科技股份有限公司 | Culture method of Chongzhi stem cell factor essence and oscillation device thereof |
| CN113846001A (en) * | 2021-10-08 | 2021-12-28 | 安徽理工大学 | Automatic detection equipment for mycobacterium tuberculosis |
| CN113846001B (en) * | 2021-10-08 | 2023-11-14 | 安徽理工大学 | Oscillator for automatic mycobacterium tuberculosis detection equipment |
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