CN112608992B - MSH6 mutant gene and application thereof - Google Patents

MSH6 mutant gene and application thereof Download PDF

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CN112608992B
CN112608992B CN202011524004.1A CN202011524004A CN112608992B CN 112608992 B CN112608992 B CN 112608992B CN 202011524004 A CN202011524004 A CN 202011524004A CN 112608992 B CN112608992 B CN 112608992B
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黄志玲
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Abstract

The invention discloses a new pathogenic gene of HR, namely an MSH6 mutant gene with g [7957inAA ] mutation, wherein the sequence of a mutant fragment of the MSH6 mutant gene is shown as SEQ ID NO: 2, respectively. Possesses the amino acid sequence of SEQ ID NO: 2, the diploid homozygous genotype of the MSH6 mutant gene with the sequence shown in the SEQ ID NO: 2 and SEQ ID NO: 1. SEQ ID NO: 3. the amino acid sequence of SEQ ID NO: 4, the diploid heterozygous genotype consisting of any MSH6 mutant gene in the sequence shown in the specification also causes the occurrence of rickets caused by low blood phosphorus in human beings, and the rickets are all in autosomal recessive inheritance. On the basis, the invention provides two mutation detection kits based on PCR capture sequencing and conventional PCR and Sanger sequencing, which have important significance for HR screening, diagnosis and birth guidance, and are particularly helpful for preventing the birth of HR children from the source. In addition, the disclosure of specific mutations in the MSH6 gene also contributes to the exploration of HR pathogenesis and the development of therapeutic drugs and methods.

Description

MSH6 mutant gene and application thereof
Technical Field
The invention relates to the field of medical molecular biology, in particular to a novel rickets low-phosphorus pathogenic gene and a detection kit thereof.
Background
Hypophosphatemic Rickets (HR) is a major type of vitamin D resistant Rickets, and impaired recovery of phosphorus from the renal tubules of HR patients will cause hypophosphatemia, and Rickets or osteomalacia will develop, and hearing and teeth will be affected. Children HR is mainly manifested by growth retardation, short stature, rickets, bone and tooth deformity, osteoporosis, walking weakness, etc.; adult HR is mainly responsible for cartilage disease and osteoarticular deformity. Biochemical examination shows that the blood phosphorus of HR patients is obviously reduced, the alkaline phosphatase is obviously increased, and rickets bone formation is shown under X-ray examination. HR is a monogenic genetic disease, the previously discovered cases mainly comprise X-sex linkage dominant inheritance, and the currently well-defined pathogenic gene is a PHEX mutant gene on the X chromosome, however, the specific pathogenic mechanism of HR is not revealed. Thus, differential diagnosis of HR requires a combination of a defined family history and genetic analysis in addition to clinical signs and biochemical examination. HR has high teratogenesis and disability rate, can seriously affect the physical health of patients, and can cause heavy burden to families and society. At present, the HR treatment mainly supplements vitamin D analogues and phosphorus agents, but has limited treatment effect, so that the basis of HR is still to follow the principle of prevention of genetic diseases, the screening of pathogenic gene carriers, the birth guidance and the prenatal diagnosis are enhanced, and the birth of HR children is avoided. Therefore, the pathogenic gene of the HR is disclosed to have important significance for the screening, diagnosis, fertility consultation and development of therapeutic drugs and methods of the HR.
The inventor collects 6 familial HR cases in advance, carries out target gene region capture sequencing on HR members and related normal members in families, and reveals that specific mutations of MSH6 gene (including 4 mutations including g. [ 7785-; through mutation fragment amplification, Sanger sequencing and family genetic analysis, 4 new MSH6 mutant genes found in HR population and non-HR population are proved to be all pathogenic genes of HR and all have autosomal recessive inheritance patterns. The MSH6 gene coding protein belongs to mismatch repair protein, also called G/T mismatch binding protein (GTBp), has the function of participating in DNA base mismatch repair, can participate in recognition and start repair after binding to mismatch sites, and jointly completes the repair process of the mismatch sites under the action of other mismatch repair related enzymes.
Disclosure of Invention
The invention provides 4 pathogenic mutant gene forms of MSH6 gene, and provides a detection kit of MSH6 mutant gene on the basis.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the object (1) of the present invention is to provide 4 mutant gene forms of MSH6, wherein the 4 mutant genes are formed by g. [ 7785-: 1-SEQ ID NO: 4, the remaining sequences are found in the reference sequence of the MSH6 wild-type gene. Possesses the amino acid sequence of SEQ ID NO: 1-SEQ ID NO: 4 and the diploid homozygous genotype of any one of the MSH6 mutant genes in the sequence set forth in SEQ ID NO: 1-SEQ ID NO: 4, any two diploid heterozygous genotypes consisting of any two MSH6 mutant genes in the sequence shown in the specification can cause the occurrence of human phosphorus-deficient rickets, and the 4 MSH6 pathogenic mutant genes are in autosomal recessive inheritance.
The object (2) of the present invention is to provide a PCR capture sequencing kit capable of simultaneously detecting g. [7785-7788delGTGA ], g. [7957inAA ], g. [12759inTTAAG ] and g. [12907inCAGC ] mutations of MSH6 gene, wherein the kit comprises the nucleotide sequences shown in SEQ ID NO: 5-SEQ ID NO: 32, and a PCR capture primer set of sequences shown in 32; and capturing sequencing products by using second-generation DNA sequencing analysis PCR to obtain mutation information.
The object (3) of the present invention is to provide a PCR kit for amplification detection of g. [7785-7788delGTGA ], g. [7957inAA ], g. [12759inTTAAG ] and g. [12907inCAGC ] mutations of MSH6 gene, wherein the kit comprises the nucleotide sequences shown in SEQ ID NO: 33-SEQ ID NO: 36, and a PCR primer pair having a sequence shown in 36; the method adopts the sequence shown in SEQ ID NO: 33-SEQ ID NO: 34, amplifying g [7785-7788delGTGA ] and g [7957inAA ] mutant fragments of the MSH6 gene; the method adopts the sequence shown in SEQ ID NO: 35-SEQ ID NO: 36 g. [12759inTTAAG ] and g. [12907inCAGC ] mutant fragments of the MSH6 gene are amplified by the sequence primer pair shown in the specification; the PCR amplification products can be directly sequenced by Sanger sequencing.
The g. [7785- ] 7788delGTGA ] mutation, g. [7957inAA ] mutation, g. [12759inTTAAG ] mutation and g. [12907inCAGC ] mutation of the MSH6 gene disclosed by the invention and the detection kit thereof are favorable for HR screening, diagnosis and fertility consultation, and are especially favorable for stopping the birth of HR children from the source; in addition, the disclosure of specific mutations in the MSH6 gene also contributes to the exploration of HR pathogenesis and the development of therapeutic drugs and methods.
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FIG. 1 is a #1 pedigree map in which boxes represent males, circles represent females, Roman letters and combinations of numbers such as I1 represent pedigree members, double horizontal lines between II1 and II2 represent married neighbors, black filled-in represents HR patients, letters AA, AA represent genotypes, wherein A represents the wild-type MSH6 gene, a represents the MSH6 mutant gene with a g. [7785 + 7788delGTGA ] mutation, + + represents the MSH6 mutant gene diploid, + represents a heterozygote, -represents the wild-type MSH6 gene homozygous, and the arrow points to the member HR prefab.
Figure 2 is a #2 pedigree map in which boxes represent males, circles represent females, roman letters and combinations of numbers such as I1 represent pedigree members, black padding represents HR patients, and letters Ac, bb, bc, Ab represent genotypes, where a represents the wild-type MSH6 gene, b represents the MSH6 mutant gene in which a g. [7957inAA ] mutation occurs, c represents the MSH6 mutant gene in which a g. [12759inTTAAG ] mutation occurs, + + represents the MSH6 mutant gene diploid, and, + represents heterozygotes.
Figure 3 is a map of a #3 family in which boxes represent males, circles represent females, roman letters and combinations of numbers such as I1 represent family members, double horizontal lines between I1 and I2 represent inbreds, black filled in represent HR patients, the letter bb represents genotype, b represents the MSH6 mutant gene in which g. [7957inAA ] mutation occurs, and, + + represents the MSH6 mutant gene diploid.
FIG. 4 is a #4 pedigree map in which boxes represent males, circles represent females, Roman letters and combinations of numbers such as I1 represent pedigree members, black padding represents HR patients, and letters Ac, cc represent genotypes, where A represents the wild-type MSH6 gene, c represents the MSH6 mutant gene with a g. [12759inTTAAG ] mutation, + represents the MSH6 mutant gene diploid, and + represents heterozygotes.
Figure 5 is a #5 pedigree map in which boxes represent males, circles represent females, roman letters and combinations of numbers such as I1 represent pedigree members, black padding represents HR patients, letters Ab, Ad, bd, dd represent genotypes, where a represents the wild-type MSH6 gene, b represents the MSH6 mutant gene in which a g. [7957inAA ] mutation occurs, d represents the MSH6 mutant gene in which a g. [12907inCAGC ] mutation occurs, + + represents the MSH6 mutant gene diploid, and + represents heterozygotes.
FIG. 6 is a #6 pedigree map in which boxes represent males, circles represent females, Roman letters and combinations of numbers such as I1 represent pedigree members, black filled-in represents HR patients, and letters AA, dd, cc, Ad, cd, Ac represent genotypes, wherein A represents the wild-type MSH6 gene, c represents the MSH6 mutant gene with a g. [12759inTTAAG ] mutation, d represents the MSH6 mutant gene with a g. [12907inCAGC ] mutation, + + represents the MSH6 mutant gene diploid, + represents heterozygote, -represents the wild-type MSH6 gene homozygote.
FIG. 7 is a Sanger sequencing map of the g. [7785-7788delGTGA ] mutation in the MSH6 mutant gene, with the arrow indicating the start of the mutation and the horizontal line indicating the 4 nucleotides GTGA that was deleted.
FIG. 8 is a diagram of family # 7in example 4. In the figure, the boxes represent males, the circles represent females, the roman alphabet and number combinations such as I1 represent family members, the black filling represents HR patients, the letters Ad, Ac, cd represent genotypes, wherein a represents the wild-type MSH6 gene, c represents the MSH6 mutant gene with a g. [12759inTTAAG ] mutation, d represents the MSH6 mutant gene with a g. [12907inCAGC ] mutation, + + represents the MSH6 mutant gene diploid, + represents a heterozygote, and the question mark represents that a febrile II2 disease condition is pending.
Detailed Description
The invention is further illustrated below with reference to specific examples.
The inventor discovers multiple MSH6 gene mutations in whole exome sequencing data of a cancer focus paraffin sample of a colorectal cancer patient in the early period, simultaneously the colorectal patient is an HR patient, HR patients also exist in a family, deep sequencing analysis discovers that HR members of the family all contain specific MSH6 gene mutations, further, the inventor firstly discloses 4 specific MSH6 mutant genes as pathogenic genes of HR in 6 HR families by adopting capture sequencing combined with family genetic analysis, and verifies the genes in a population sample.
Example 1 discovery of specific MSH6 mutant Gene as a causative Gene of HR
FIG. 1 shows the HR pedigree with the earliest mutation in MSH6 gene (pedigree #1), II1 and II2 as close mates (II1 is solitary and the parents have died). Wherein, the brothers III1 and III2 both present the symptoms of short stature and rickets, and have the characteristics of hypophosphatemia, urine phosphorus increase and the like, and are clinically diagnosed as HR. In addition to HR, III1 (proband, marked with arrows) was diagnosed as colorectal cancer stage III at 24 years of 2018, with surgical resection of the foci, however recurrence occurred in the second half of the surgery. Family history survey revealed that both III1 and III2 in family #1 were HR patients, and III1 was confirmed to be colorectal cancer, with certain genetic indications and predisposition to disease. To guide subsequent treatment of colorectal cancer patients III1 and to analyze potential genetic causative factors and assess the risk of developing infants with an in-family regenerative HR (mainly the marriage of son III1 and daughter III2 of II2 and the re-birth of sister II3 of II2), blood samples of II1, II2, III1 and III2 and post-operative cancer paraffin tissue samples of III1 (10 slices of 1cm × 1cm pathological FFPE sections) were first collected with informed consent of the patient's family and sent to jieky genomics limited to perform whole exome sequencing analysis. The basic flow is as follows: genomic DNA was extracted from Blood and paraffin samples using the QIAamp DNA Blood Mini Kit and the QIAamp DNA FFPE Tissue Kit from Qiagen, Germany, respectively, whole Exome capture was performed using the SeqCap EZ Exome Kit V3 Kit from Roche, USA, and sequencing was performed on a HiSeq 4000 second generation sequencer (Illumina, USA) to obtain gene mutation information by comparison with the human reference genome hg 19. The sequencing depth of the blood sample was required to be >50 x and the sequencing depth of the paraffin sample was required to be >250 x.
The final sequencing results showed > 99.5% coverage of the target capture area, 64 x mean sequencing depth for blood samples, 281 x mean sequencing depth for paraffin section samples. For III1, the DNA sequencing information of the excised cancer focus sample was analyzed with the genomic DNA information (i.e. its own genome) measured from the blood sample and the human reference genome hg19 as references, and as a result, ten kinds of major gene mutations (mutation ratio > 1%) were found, including the g. [7785-7788delGTGA ] mutation of the MSH6 gene, which also existed in the genomic DNA (the wild-type MSH6 gene and the g. [7785-7788delGTGA ] mutant MSH6 gene were measured from the blood sample at the same time, and both of them were about 50% and substantially both were alleles). The genome results of II1, II2 and III2 measured by blood samples show that g. [7785-7788delGTGA ] mutation of the MSH6 gene also exists in the genomes of the three genes, the difference is that III1 and III2 only detect g. [7785-7788delGTGA ] mutant MSH6 gene, and II1 and II2 both detect the wild-type MSH6 gene and g. [7785-7788delGTGA ] mutant MSH6 gene (and the wild-type and mutant genes account for about 50% respectively). All samples with MSH6 gene g. [7785-7788delGTGA ] mutation detected by whole exome capture sequencing were subjected to Sanger sequencing to verify the detection result of the g. [7785-7788delGTGA ] mutation. Besides the g. [ 7785-7788-delGTGA ] mutant MSH6 gene shared with III1, no other significant mutations were detected in the II1, II2 and III2 genomes, namely the g. [ 7785-7788-delGTGA ] mutant MSH6 gene exists in all members of the two generations of the HR family, so that the g. [ 7785-7788-delGTGA ] mutant MSH6 gene is presumed to be the pathogenic gene of HR, and the distribution of the gene in the family #1 is further detected.
Blood samples of members I1, I2, II3, II4 and III3 of pedigree #1 were collected and tested for mutations in the MSH6 gene. In order to save cost, PCR capture sequencing aiming at the MSH6 gene is adopted for further detection. The sequence in the range of 47783145-47806954 in the DNA fragment of NCBI accession No. NC-000002.12 (position 47783145 is position 1 of MSH6 gene, containing the starting point and the ending point of 23810bp) is the reference sequence of human MSH6 gene (cut-off 2020.11.1 information), and on the basis of the sequencing result of the early whole exome of family #1 member, the PCR capture probes shown in Table 1 are designed to carry out capture sequencing analysis based on multiplex PCR on the MSH6 gene. The target region of the MSH6 gene captured by the PCR capture primer set shown in Table 1 is not limited to the region where the 4 mutations occur, but covers all exon regions and exon-intron junction regions of the MSH6 gene, so as to fully discover other mutations of the MSH6 gene. The multiplex PCR amplification system (reagents of Life tech, USA, refer to the method of the system) and conditions are shown in Table 2, the multiplex PCR amplification products are sent to Shanghai Bioengineering Co., Ltd for second-generation sequencing analysis, and the mutations detected need to be subjected to Sanger sequencing verification (g. [ 7785; 7788. delGTGA ] Sanger sequencing map at the mutation site is shown in FIG. 7). The mutation of MSH6 gene in family #1 is shown in Table 3.
TABLE 1 PCR Capture sequencing primer set for MSH6 Gene
Amplicon numbering Primer upstream sequence (5 '-3') Upstream sequence identification Primer downstream sequence (5 '-3') Downstream sequence identification
Amplicon1 AGATTTCCCGCCAGCAGGAG SEQ ID NO:5 CTGCACTCATTCAAGCCAACTC SEQ ID NO:6
Amplicon2 CTTTTGGAGGGAGGAGACGC SEQ ID NO:7 CTACCTACCGAAGGACCCAG SEQ ID NO:8
Amplicon3 TGTAGGTAACTGCCTTTAAGGA SEQ ID NO:9 GTCTGCCTGTCTGTCTGTTTC SEQ ID NO:10
Amplicon4 CTTGAACTGCTGGGATTACAG SEQ ID NO:11 CAGGGAACTACAGAAGTATGC SEQ ID NO:12
Amplicon5 AGTTGAACTGTCTTACATTATGG SEQ ID NO:13 CTAACGTGGGCTTGGGATTCA SEQ ID NO:14
Amplicon6 TGGAGGTGGTGATGACAGTAG SEQ ID NO:15 TTCTCAGAGGGATCACCTTCCA SEQ ID NO:16
Amplicon7 CTACAGTAAGTATCTTCTTAGCC SEQ ID NO:17 GACTGTGTCAGAATCCAAGGGA SEQ ID NO:18
Amplicon8 AGCACTACAAGATCTGGTGCTA SEQ ID NO:19 ATCAGGAAAACGACCTTCAGGA SEQ ID NO:20
Amplicon9 TTGACTGTAGAATTGAACCGATG SEQ ID NO:21 ACAAGCTTGTTCAAAGTCTTACC SEQ ID NO:22
Amplicon10 ATGAAGCCTCACTTTTACCCTC SEQ ID NO:23 ACTGTGTTTGGAAAATGATCACC SEQ ID NO:24
Amplicon11 GACCTTTTCCTCCCTCATTCAC SEQ ID NO:25 AATTATTGGCCGGGCACGGTT SEQ ID NO:26
Amplicon12 GCTCATGATAGCTATATAACCTA SEQ ID NO:27 TCCAACTATCGGTCTGTGCCA SEQ ID NO:28
Amplicon13 CTTTAACAGGAAGAGGTACTGC SEQ ID NO:29 GTTAGTTAGTTACCGAAATAATCG SEQ ID NO:30
Amplicon14 CTAACTGACCTTAAGTTTCAAAG SEQ ID NO:31 CCACCTTTGTCAGAAGTCAAC SEQ ID NO:32
TABLE 2PCR Capture amplification System and conditions
Figure BDA0002849435890000061
Figure BDA0002849435890000071
TABLE 3 mutation of MSH6 Gene in family #1
Figure BDA0002849435890000072
As can be seen from Table 3, in the #1 family, diseases were caused only in the case of the transgenic line containing the homozygous mutant gene of MSH6 (g. [7785-7788delGTGA ] mutation) (III1 and III2), while the transgenic line carrying both the MSH6 mutant gene and the wild-type MSH6 gene did not cause diseases (I1, II1, II2, II3 and III3), and the transgenic line containing only the wild-type MSH6 gene did not cause diseases (I2 and II4), and the pathogenic mutant genes were both transmitted from parents to daughter, and conformed to the autosomal recessive inheritance pattern (regardless of sex, and the MSH6 gene was located on autosomal chromosome 2), i.e., the MSH6 mutant gene was the recessive pathogenic gene and the MSH6 mutant gene and the MSH6 wild-type gene were allelic to each other. Assuming that the wild-type gene of MSH6 is A and the mutant gene of MSH6 is a, the genotypes of the individual members of pedigree #1 are shown in FIG. 1 and Table 3 according to the gene detection results. It can be seen from the gene transmission rule of the pedigree in FIG. 1 that it is typical autosomal recessive inheritance, the phenotype and genotype are consistent, i.e., the genotype determines the phenotype, and the phenotype co-segregates with the genotype. As a result, the g [7785-7788delGTGA ] type MSH6 mutant gene is a new autosomal recessive pathogenic gene of HR (not shown). On the basis, HR families are further collected for verification.
Example 2 extended family verification
Further, 5HR families (nos. #2- #6) were collected, satisfying that there were more than 2 HR members in the families, and the pedigree map shows the possibility of having autosomal recessive inheritance. The MSH6 gene mutation assay was performed on blood samples of HR patients and related members in the pedigree according to the PCR capture sequencing method of example 1 (tables 1 and 2) and the results are shown in Table 4 (pedigree #1 results from example 1 are also included for convenience of summary analysis).
TABLE 46 mutation profiles of the HR pedigree MSH6 Gene
Figure BDA0002849435890000081
From the MSH6 gene detection of families #2- #6, 3 new mutation types were found, which are g. [7957inAA ], g. [12759inTTAAG ] and g. [12907inCAGC ] (verified by Sanger sequencing), and the 3 MSH6 mutant genes were analyzed to be pathogenic genes of HR (see Table 4 and FIG. 2-FIG. 6). In combination with the family #1 marker of example 1, the wild-type MSH6 gene is marked with A, the g. [ 7785-plus 7788delGTGA ] mutant MSH6 gene is marked with a, and the g. [7957inaA ], g. [ 12759-INTTAAG ] and g. [ 12907-inCAGC ] mutant MSH6 genes, the wild-type MSH6 gene and g. [ 7785-plus 7788delGTGA ], g. [ 7957-inaA ], g. [ 12759-inTTAAG ] and g. [ 12907-inCAGC ] are alleles of each other among the 4 mutant MSH6 genes. The specific analysis is as follows:
for family #2, HR patient II1 simultaneously detected g. [7957inAA ] and g. [12759inTTAAG ] mutations of the MSH6 gene, belonging to a compound heterozygous mutant genotype (bc), inherited from its father I1 (g. [12759inTTAAG ] mutation of the MSH6 gene, genotype Ac) and mother II2 (g. [7957inAA ] mutation of the MSH6 gene, genotype bb), respectively, whose mother II2 was the homozygous mutant genotype (bb), was also an HR patient. Therefore, the HR of the family #2 conforms to the genotype to determine the phenotype, the phenotype is co-segregated along with the genotype, and the inheritance pattern of the HR virulence gene is autosomal recessive inheritance. From this, it is known that the g. [7957inAA ] and g. [12759inTTAAG ] mutant MSH6 genes are both autosomal recessive pathogenic genes of HR.
Family #3 was analyzed similarly to family #2, I1 and I2 in family #3 were married closely, I1, I2 and II1 all detected g. [7957inAA ] mutation containing MSH6 gene, these 3 were all homozygous mutant genotypes (genotype bb), and father I1 and mother I2 of HR patient II1 each inherited a disease-causing gene mutation to II1 to cause disease. Therefore, the HR of the family #3 accords with the genotype to determine the phenotype, the phenotype is co-segregated along with the genotype, and the genetic pattern of the HR virulence gene is autosomal recessive inheritance. From this, it was found that the g. [7957inAA ] mutant MSH6 gene is an autosomal recessive pathogenic gene of HR.
Family #4, similar to family #2 analysis, HR patients II1 and II2 both detected g. [12759inTTAAG ] homozygous mutation (genotype cc) in the MSH6 gene, and the disease-causing gene mutation was inherited from their father (genotype Ac) and mother (genotype Ac), respectively. Therefore, the HR of the family #4 accords with the genotype to determine the phenotype, the phenotype is co-segregated along with the genotype, and the genetic pattern of the HR virulence gene is autosomal recessive inheritance. From this, the g. [12759inTTAAG ] mutant MSH6 gene was found to be an autosomal recessive pathogenic gene of HR.
Pedigree #5 is similar to pedigree #2 analysis in that HR patients II1 and II2 both detected g. [7957inAA ] and g. [12907inCAGC ] mutations in the MSH6 gene, belonging to a complex heterozygous mutant genotype (bd), inherited from their father I1 (g. [12907inCAGC ] mutation in the MSH6 gene, genotype dd) and mother II2 (g. [7957inAA ] mutation in the MSH6 gene, genotype Ab), respectively, whose father I1 was the homozygous mutant genotype (dd), and were also HR patients. Therefore, the HR of the family #5 accords with the genotype to determine the phenotype, the phenotype is co-segregated along with the genotype, and the genetic pattern of the HR virulence gene is autosomal recessive inheritance. From this, it was found that the g. [7957inAA ] and g. [12907inCAGC ] mutant MSH6 genes are all causative genes of HR.
Pedigree #6 is similar to pedigree #2 in analysis, and HR patient III1 detected g. [12759inTTAAG ] and g. [12907inCAGC ] mutations containing both MSH6 genes, belonging to a complex heterozygous mutant genotype (cd), inherited from her mother II1 (g. [12759inTTAAG ] mutation of MSH6 gene, genotype cc, HR patient) and father II2 (g. [12907inCAGC ] mutation of MSH6 gene, genotype Ad), respectively; furthermore, the disease-causing gene mutation in father II2 of III1 (g. [12907inCAGC ] mutation in MSH6 gene) was inherited from I2 (genotype dd, HR patients). Therefore, the HR of the family #6 accords with the genotype to determine the phenotype, the phenotype is co-segregated along with the genotype, and the genetic pattern of the HR virulence gene is autosomal recessive inheritance. From this, it was found that the g. [12759inTTAAG ] and g. [12907inCAGC ] mutant MSH6 genes were all causative genes of HR.
Through PCR capture sequencing targeted to the MSH6 gene, 3 new mutations, namely g. [7957inAA ], g. [12759inTTAAG ] and g. [12907inCAGC ] were found, and the HR of the families #2 to #6 can be genetically analyzed. For the sake of completeness, all HR patients in these 5 families were selected for whole exome sequencing validation and the type and proportion of mutant genes were found to be consistent with the results of PCR capture sequencing. Taking HR patient II1 of family #2 as an example, the whole exome sequencing also found only g. [7957inAA ] and g. [12759inTTAAG ] mutations of MSH6 gene (except that no other obvious gene mutations were found), and both accounted for about 50% each, which is consistent with the results of PCR capture sequencing for MSH6 gene. Thus, it was confirmed that the MSH6 gene of a specific mutation type is a causative gene of HR.
In conclusion, the MSH6 mutant genes with g. [7785-7788delGTGA ], g. [7957inAA ], g. [12759inTTAAG ] and g. [12907inCAGC ] mutations are all pathogenic genes of HR, and any homozygous mutation or any compound heterozygous mutant genotype can cause the generation of HR and all are autosomal recessive inheritance. According to the literature, the MSH6 gene codes mismatch repair protein, has the function of participating in DNA base mismatch repair, can participate in recognition and starts repair after being combined to mismatch sites, and then completes the repair process of the mismatch sites together under the action of other mismatch repair related enzymes. The MSH6 gene has been shown to play a role in the development of tumor, however, there is no research report showing that the gene is related to HR, and there is no research report that the gene is related to the absorption and metabolism of phosphorus in vivo. Notably, the overall incidence of tumors was significantly higher than the average in the normal population in HR families and populations collected by the inventors containing mutations in the MSH6 gene.
Example 3HR population sample validation
On the basis of examples 1 and 2, the mutation of the MSH6 gene in the HR population is further detected. 27 clinically confirmed HR patients (accession numbers S1-S27), 16 male patients, 11 female patients, aged 26.3 years (4-57 years) were collected. Following the PCR capture sequencing method of example 1 (tables 1 and 2) with sufficient informed consent of these HR patients, blood samples (peripheral venous anticoagulation 2ml) were taken for MSH6 gene mutation testing, with the results shown in table 5. Of these, only the HR patients from S1 to S17 detected specific mutations containing the MSH6 gene, while none of the HR patients from S18 to S27 detected any mutation containing the MSH6 gene, indicating that there are other known or unknown mutations of the gene that could lead to the development of HR (e.g., PHEX mutant gene on the X chromosome). Only g. [7785-7788delGTGA ], g. [7957inAA ], g. [12759inTTAAG ] and g. [12907inCAGC ] mutations of the MSH6 gene were detected in HR patients from S1 to S17, and mutations at other sites of the MSH6 gene were not detected. Wherein 5HR patients contained homozygous mutations in 3 MSH6 genes (S1, S9, S13-S15), involving genotypes aa, bb and cc; the remaining 12 HR patients contained a composite heterozygous mutant combination of all 6 MSH6 genes, involving genotypes including ab, ac, ad, bc, bd and cd, see in particular table 5.
TABLE 5 mutation of MSH6 Gene in HR patient population
Figure BDA0002849435890000111
Note: the + number in Table 5 indicates that the corresponding mutation was detected, and the null or-indicates that no mutation was detected.
PCR primers were designed to amplify the mutated gene fragments for the 4 specific mutations of the MSH6 genes detected in the HR patients from S1-S17 in Table 5, and the amplification primers and conditions were as shown in Table 6 (the amplification system was referred to as a conventional PCR system), wherein the g. [ 7785-. And (3) verifying the PCR amplification product of the mutant gene fragment by agarose gel electrophoresis, and then sending the PCR amplification product to Shanghai biological engineering Co., Ltd for Sanger sequencing verification. (Sanger sequencing validation of mutation results by Whole exome capture sequencing and PCR capture sequencing, PCR amplification and detection methods as described in examples 1 and 2). Blood samples of 250 normal populations (non-HR patients) were collected as reference samples, 2ml of anticoagulated peripheral vein was collected with full informed consent, and amplification of specific gene fragment of MSH6 and Sanger sequencing were performed using the PCR system and conditions shown in Table 6. Sanger sequencing results show that 4 specific mutations of the MSH6 gene were detected in HR patients from S1 to S17 and completely consistent with PCR capture sequencing results (including completely consistent mutation types and proportions, Table 5), while 4 specific mutations of the MSH6 gene were not found in 250 normal populations. It follows that the 4 specific mutations of the MSH6 gene are only present in HR patients and their related family members, and not in normal populations other than HR.
TABLE 6PCR primers and amplification conditions
Figure BDA0002849435890000121
Example 4 use of specific MSH6 mutant genes
As shown in FIG. 8, in the family (family #7), both couples and marrows were normal, and a child with HR was born and then a reproduction was planned for a birth heredity consultation. Collecting blood samples of a couple and an HR infant, carrying out whole exome capture sequencing, and finding that father I1 has g. [12907inCAGC ] mutation (heterozygote, genotype is Ad) of an MSH6 gene, mother I2 has g. [12759inTTAAG ] mutation (heterozygote, genotype is Ac) of an MSH6 gene, and HR infant II1 has g. [12759inTTAAG ] mutation and g. [12907inCAGC ] mutation (compound heterozygote, genotype is cd) of an MSH6 gene, wherein the two MSH6 gene mutations are respectively inherited from parent and father; no other obvious genetic mutations were detected in members of this family, except for the 2 specific mutations in the MSH6 gene. The pedigree accords with the genotype-determining phenotype and the phenotype realizes the cosegregation along with the genotype, and the results of combining the examples 1-3 show that the MSH6 mutant gene is the pathogenic gene of HR and is in autosomal recessive inheritance.
According to the law of gene isolation and free combination, the probability of the child suffering from HR is 1/4 (genotype cd), the probability of the child bearing carrier is 1/2 (genotype Ac or Ad, no expression of HR phenotype), and the probability of the child bearing healthy person is 1/4 (genotype AA). Thus, the couple decided to give birth again, and the mother collected peripheral blood (10ml) at 14 weeks gestation in second fetus, extracted extracellular free DNA (QIAGEN, Germany), and examined by whole exon capture sequencing according to example 1, and did not find any mutation in the MSH6 gene (only the wild-type MSH6 gene was detected), so the pregnancy continued until live birth, and finally a female infant was biopsied. The infant heel blood was collected, and the female infant was tested for MSH6 gene mutation by PCR capture sequencing (see example 1), resulting in no detection of any MSH6 gene mutation. No HR symptoms were observed at approximately 2 years of age with follow-up. Therefore, the MSH6 gene as a newly disclosed HR pathogenic gene has great significance for HR screening and diagnosis and HR family fertility guidance.
In conclusion, the 4 MSH6 mutant genes with specific mutations of g. [7785-7788delGTGA ], g. [7957inAA ], g. [12759inTTAAG ] and g. [12907inCAGC ] disclosed by the invention are all novel pathogenic genes of HR, wherein any one homozygous mutant genotype or any one compound heterozygous mutant genotype can cause the generation of HR and are all in autosomal recessive inheritance; on the basis, two mutation detection kits based on PCR capture sequencing and conventional PCR and Sanger sequencing are provided, have important significance on HR screening, diagnosis and birth guidance, and are particularly helpful for stopping the birth of HR children from the source. In addition, the disclosure of specific mutations in the MSH6 gene also contributes to the exploration of HR pathogenesis and the development of therapeutic drugs and methods.
Sequence listing
<110> Dingzhiling
<120> MSH6 mutant gene and application thereof
<160> 36
<170> SIPOSequenceListing 1.0
<210> 1
<211> 244
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 1
taactaagtt atgtatttcc ttttggcaac agttcttctc accaggagat ttggtttggg 60
ccaagatgga gggttacccc tggtggcctt gtctggttta caaccacccc tttgatggaa 120
cattcatccg cgagaaaggg aaatcagtcc gtgttcatgt acagtttttt gatgacagcc 180
caacaagggg ctgggttagc aaaaggcttt taaagccata tacaggtaag agtcactact 240
gcca 244
<210> 2
<211> 250
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 2
taactaagtt atgtatttcc ttttggcaac agttgtgact tctcaccagg agatttggtt 60
tgggccaaga tggagggtta cccctggtgg ccttgtctgg tttacaacca cccctttgat 120
ggaacattca tccgcgagaa agggaaatca gtccgtgttc atgtacagtt ttttgatgac 180
agcccaacaa ggggctgggt tagcaaaaaa ggcttttaaa gccatataca ggtaagagtc 240
actactgcca 250
<210> 3
<211> 267
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 3
attacaggcg tgagcctctg cacccggccc ttattgttta taaatacatt tctttctagg 60
ttcaaaattt aagcaaagga agcccagaag ggaggtcatt tttacagtgc aaagcctgaa 120
atactgagag caatgcaacg tgcagatgaa gccttaaata aagacaagat taagaggctt 180
gaattggcag tttgtgatga gccctcagag ccagaagagg aagaagagat ggaggtggga 240
cacggcaagc attcagttgt tatttat 267
<210> 4
<211> 266
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 4
attacaggcg tgagcctctg cacccggccc ttattgttta taaatacatt tctttctagg 60
ttcaaaatca aaggaagccc agaagggagg tcatttttac agtgcaaagc ctgaaatact 120
gagagcaatg caacgtgcag atgaagcctt aaataaagac aagattaaga ggcttgaatt 180
ggcagtttgt gatgagccct cagagccaga agaggaacag cgaagagatg gaggtgggac 240
acggcaagca ttcagttgtt atttat 266
<210> 5
<211> 20
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 5
agatttcccg ccagcaggag 20
<210> 6
<211> 22
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 6
ctgcactcat tcaagccaac tc 22
<210> 7
<211> 20
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 7
cttttggagg gaggagacgc 20
<210> 8
<211> 20
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 8
ctacctaccg aaggacccag 20
<210> 9
<211> 22
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 9
tgtaggtaac tgcctttaag ga 22
<210> 10
<211> 21
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 10
gtctgcctgt ctgtctgttt c 21
<210> 11
<211> 21
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 11
cttgaactgc tgggattaca g 21
<210> 12
<211> 21
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 12
cagggaacta cagaagtatg c 21
<210> 13
<211> 23
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 13
agttgaactg tcttacatta tgg 23
<210> 14
<211> 21
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 14
ctaacgtggg cttgggattc a 21
<210> 15
<211> 21
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 15
tggaggtggt gatgacagta g 21
<210> 16
<211> 22
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 16
ttctcagagg gatcaccttc ca 22
<210> 17
<211> 23
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 17
ctacagtaag tatcttctta gcc 23
<210> 18
<211> 22
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 18
gactgtgtca gaatccaagg ga 22
<210> 19
<211> 22
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 19
agcactacaa gatctggtgc ta 22
<210> 20
<211> 22
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 20
atcaggaaaa cgaccttcag ga 22
<210> 21
<211> 23
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 21
ttgactgtag aattgaaccg atg 23
<210> 22
<211> 23
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 22
acaagcttgt tcaaagtctt acc 23
<210> 23
<211> 22
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 23
atgaagcctc acttttaccc tc 22
<210> 24
<211> 23
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 24
actgtgtttg gaaaatgatc acc 23
<210> 25
<211> 22
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 25
gaccttttcc tccctcattc ac 22
<210> 26
<211> 21
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 26
aattattggc cgggcacggt t 21
<210> 27
<211> 23
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 27
gctcatgata gctatataac cta 23
<210> 28
<211> 21
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 28
tccaactatc ggtctgtgcc a 21
<210> 29
<211> 22
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 29
ctttaacagg aagaggtact gc 22
<210> 30
<211> 24
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 30
gttagttagt taccgaaata atcg 24
<210> 31
<211> 23
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 31
ctaactgacc ttaagtttca aag 23
<210> 32
<211> 21
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 32
ccacctttgt cagaagtcaa c 21
<210> 33
<211> 20
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 33
taactaagtt atgtatttcc 20
<210> 34
<211> 18
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 34
tggcagtagt gactctta 18
<210> 35
<211> 18
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 35
attacaggcg tgagcctc 18
<210> 36
<211> 21
<212> DNA
<213> Intelligent (Homo sapiens)
<400> 36
ataaataaca actgaatgct t 21

Claims (1)

1. An MSH6 mutant gene, characterized in that the MSH6 mutant gene is a pathogenic gene of low blood phosphorus rickets, and the sequence of a mutant fragment of the MSH6 mutant gene is shown as SEQ ID NO: 2, it is formed by g. [7957inAA ] mutation of wild-type MSH6 gene.
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19909878A1 (en) * 1999-03-06 2000-09-07 Univ Dresden Tech Novel variants of the human mismatch repair gene, MSH6, useful e.g. for determining predisposition to cancer and for development of drugs
WO2016183486A1 (en) * 2015-05-13 2016-11-17 Agenus Inc. Vaccines for treatment and prevention of cancer
CN106282351A (en) * 2016-08-22 2017-01-04 南京医科大学附属南京儿童医院 X chain hypophosphatemic rickets Disease-causing gene abrupt climatic change primer and application thereof
CN107236037A (en) * 2016-03-29 2017-10-10 博奥颐和健康科学技术(北京)有限公司 The MSH6 albumen and its encoding gene of a kind of mutation, application

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19909878A1 (en) * 1999-03-06 2000-09-07 Univ Dresden Tech Novel variants of the human mismatch repair gene, MSH6, useful e.g. for determining predisposition to cancer and for development of drugs
WO2016183486A1 (en) * 2015-05-13 2016-11-17 Agenus Inc. Vaccines for treatment and prevention of cancer
CN107847572A (en) * 2015-05-13 2018-03-27 艾吉纳斯公司 Vaccine for treatment of cancer and prevention
CN107236037A (en) * 2016-03-29 2017-10-10 博奥颐和健康科学技术(北京)有限公司 The MSH6 albumen and its encoding gene of a kind of mutation, application
CN106282351A (en) * 2016-08-22 2017-01-04 南京医科大学附属南京儿童医院 X chain hypophosphatemic rickets Disease-causing gene abrupt climatic change primer and application thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
"A novel c.2179T > C mutation blocked the intracellular transport of PHEX protein and caused X-linked hypophosphatemic rickets in a Chinese family";Li等;《MOLECULAR GENETICS & GENOMIC MEDICINE》;20200617;第8卷(第8期);全文 *
"Biochemical basis for dominant mutations in the Saccharomyces cerevisiae MSH6 gene";Hess等;《PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA》;20060117;第103卷(第3期);全文 *
中国人遗传性非息肉病性结直肠癌MSH6基因胚系突变的测序研究;颜士岩等;《中华医学遗传学杂志》;20071210(第06期);全文 *
家族性低磷血症性佝偻病家系X染色体内肽酶同源性的磷酸调节基因新突变一例报道;李媛等;《中国全科医学》;20180820(第24期);全文 *

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