CN112595685A - A product containing cecropin and method for detecting cecropin content in the product - Google Patents
A product containing cecropin and method for detecting cecropin content in the product Download PDFInfo
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- CN112595685A CN112595685A CN202011597906.8A CN202011597906A CN112595685A CN 112595685 A CN112595685 A CN 112595685A CN 202011597906 A CN202011597906 A CN 202011597906A CN 112595685 A CN112595685 A CN 112595685A
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- 108050004290 Cecropin Proteins 0.000 title claims abstract description 84
- 238000000034 method Methods 0.000 title claims abstract description 29
- 239000000843 powder Substances 0.000 claims abstract description 13
- 239000000126 substance Substances 0.000 claims abstract description 11
- 238000001914 filtration Methods 0.000 claims abstract description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 34
- 239000000243 solution Substances 0.000 claims description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- 239000011259 mixed solution Substances 0.000 claims description 15
- 239000000654 additive Substances 0.000 claims description 7
- 230000010355 oscillation Effects 0.000 claims description 7
- 238000009210 therapy by ultrasound Methods 0.000 claims description 7
- 235000007164 Oryza sativa Nutrition 0.000 claims description 6
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 claims description 6
- 230000000996 additive effect Effects 0.000 claims description 6
- 235000009566 rice Nutrition 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 4
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 3
- 229920000053 polysorbate 80 Polymers 0.000 claims description 3
- 238000002798 spectrophotometry method Methods 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims 1
- 241001465754 Metazoa Species 0.000 abstract description 11
- 239000003674 animal food additive Substances 0.000 abstract description 4
- 238000011161 development Methods 0.000 abstract description 4
- 230000001737 promoting effect Effects 0.000 abstract 1
- 239000000047 product Substances 0.000 description 40
- 238000001514 detection method Methods 0.000 description 7
- 241000209094 Oryza Species 0.000 description 5
- 229940126680 traditional chinese medicines Drugs 0.000 description 4
- 239000003814 drug Substances 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000010903 husk Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/14—Preparation by elimination of some components
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
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- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The invention provides a product containing cecropin and a method for detecting the content of the cecropin in the product, and the product containing the cecropin comprises the cecropin and a water-insoluble substance, wherein the water-insoluble substance comprises corncob powder. The method for detecting the content of cecropin in the product comprises the following steps of (1) dissolving cecropin in the product by using a dissolving solution; (2) filtering or centrifuging to obtain solution containing cecropin; (3) and (5) measuring the content of cecropin in the solution. The product containing cecropin can be used as feed additive for improving animal resistance and promoting animal growth and development. By the method for detecting the content of the cecropin in the product, whether the addition amount of the cecropin in the product is qualified or not can be quickly detected, and delivery of unqualified products is reduced.
Description
Technical Field
The invention relates to cecropin, in particular to a product containing cecropin and a method for detecting the content of the cecropin in the product.
Background
In the prior art, animals, particularly poultry and livestock are prevented from diseases by using antibiotics, but the antibiotics can improve the drug resistance, and along with the requirement of people on antibiotic-free products, people start to prevent diseases of the animals by using traditional Chinese medicine additives, but because the planting amount of traditional Chinese medicines is small, the traditional Chinese medicines are different in different planting conditions and different in drug properties of the traditional Chinese medicines in different years, the difference of the drug properties of the traditional Chinese medicines is large, and the disease prevention and the prevention of the animals are influenced. The application provides a product containing cecropin, which can be used as a feed additive to improve the resistance of animals and promote the growth and development of the animals. However, when the product is subjected to quality detection and quantitative detection, the product is dissolved by water, and then the supernatant is centrifuged to be detected, so that the detection result is far lower than the theoretical content, only 40% -60% of the theoretical content can be detected, the daily quantitative detection requirement on the product cannot be met, and the experimenters continuously improve the method, so that the content detection accuracy cannot be improved all the time. Finally, through long-time observation and research, a method for detecting the content of cecropin in the product is provided, and the detection requirement can be met.
Disclosure of Invention
In order to solve the problems, the invention provides a product containing cecropin and a method for detecting the content of the cecropin in the product, and the product containing the cecropin can be used as a feed additive to improve the resistance of animals and promote the growth and development of the animals. By the method for detecting the content of the cecropin in the product, whether the addition amount of the cecropin in the product is qualified or not can be quickly detected, and delivery of unqualified products is reduced.
The object of the invention is achieved in the following way: a product containing cecropin comprises cecropin and water insoluble substance, wherein the water insoluble substance comprises corncob powder.
The water-insoluble substance also comprises at least one of Maifanitum and rice hull powder.
The method for detecting the content of cecropin in the product comprises the following steps of (1) dissolving cecropin in the product by using a dissolving solution; (2) filtering or centrifuging to obtain solution containing cecropin; (3) and (5) measuring the content of cecropin in the solution.
In the step (3), the content of cecropin in the solution is determined by using an ultraviolet spectrophotometry.
In the step (3), the content of cecropin in the solution is measured by using a high performance liquid chromatograph.
The dissolving solution is a mixed solution of water and an additive for reducing the tension of the water.
The additive for reducing water tension is at least one of ethanol, trifluoroacetic acid and tween 80.
The dissolving solution is a mixed solution of water and ethanol, and the volume fraction of the ethanol in the mixed solution is 30-60%.
The dissolving process of the step (1) also comprises vortex oscillation and ultrasonic treatment.
Vortex and shake for 30min, and sonicate for 30 min.
Compared with the prior art, the product containing cecropin and the method for detecting the content of the cecropin in the product can be used as a feed additive to improve the resistance of animals and promote the growth and development of the animals. By the method for detecting the content of the cecropin in the product, whether the addition amount of the cecropin in the product is qualified or not can be quickly detected, and delivery of unqualified products is reduced.
Detailed Description
A product containing cecropin comprises cecropin and water insoluble substance, wherein the water insoluble substance comprises corncob powder.
The water-insoluble substance also comprises at least one of Maifanitum and rice hull powder. The method for detecting the content of cecropin in the product comprises the following steps of (1) dissolving cecropin in the product by using a dissolving solution; (2) filtering or centrifuging to obtain solution containing cecropin; (3) and (5) measuring the content of cecropin in the solution.
In the step (3), the content of cecropin in the solution is determined by using an ultraviolet spectrophotometry.
In the step (3), the content of cecropin in the solution is measured by using a high performance liquid chromatograph.
The dissolving solution is a mixed solution of water and an additive for reducing the tension of the water.
The additive for reducing water tension is at least one of ethanol, trifluoroacetic acid and tween 80.
The dissolving solution is a mixed solution of water and ethanol, and the volume fraction of the ethanol in the mixed solution is 30-60%.
The dissolving process of the step (1) also comprises vortex oscillation and ultrasonic treatment.
Vortex and shake for 30min, and sonicate for 30 min.
The present invention is described in detail below with reference to specific embodiments, it should be noted that the embodiments are only used for further illustration of the present invention, and should not be construed as limiting the scope of the present invention, and those skilled in the art can make modifications and adaptations of the present invention based on the above-mentioned disclosure.
The following examples will be given by examining a product containing cecropin, consisting of cecropin, maifanite, and rice hull powder. Uniformly stirring and mixing cecropin raw powder with known content in a certain weight with corn cob powder, medical stone and rice husk powder (the ratio of the corn cob powder to the medical stone to the rice husk powder is 1: 1: 1) to obtain a product containing cecropin, wherein the content of the cecropin in the product containing the cecropin is 2000 ug/g by calculation, namely the theoretical content of the cecropin in the product containing the cecropin is 2000 ug/g.
Randomly selecting 5 batches of products, dissolving by adopting the following different dissolving solutions, centrifuging, taking supernate, and detecting the cecropin content in the products by using a high performance liquid chromatograph (Agilent 1260).
(1) Dissolving the product containing cecropin by using a mixed solution of water and ethanol, performing vortex oscillation for 30min and ultrasonic treatment for 30min in the dissolving process, wherein the content measurement results of the cecropin in the product containing cecropin are shown in the following tables 1 and 2 under the condition that the ethanol in the mixed solution has different volume percentage contents.
TABLE 2
From the results of tables 1 and 2, 30% ethanol was found to be the most preferable solvent. The solvent has good dissolving effect, the recovery rate can reach 100%, and the cost of 30% ethanol is the lowest compared with other solvents with the recovery rate of 100%.
(2) Dissolving the product containing cecropin by using a mixed solution of water and ethanol, wherein the volume percentage of the ethanol in the mixed solution is 30%, performing vortex oscillation for 30min and ultrasonic treatment for 30min during the dissolving process, and the measurement result of the content of the cecropin in the product containing the cecropin is shown in the following table 3.
TABLE 3
The results are given in table 3: vortex oscillation for 30min, and ultrasonic treatment for 30min are very necessary in the dissolving step, and the cecropin can be fully dissolved only by treating the two steps in place.
(3) The original dissolving method adopts water as dissolving solution; in the application, a product containing cecropin is dissolved by using a mixed solution of water and ethanol, wherein the volume percentage of the ethanol in the mixed solution is 30%, and whether vortex oscillation and ultrasonic treatment are performed for 30min or not in the dissolving process is performed for 30 min; the results of measuring the content of cecropin in the cecropin-containing product are shown in table 4 below.
TABLE 4
The results in table 4 show that the detection method greatly improves the dissolution rate of cecropin, so that the cecropin in the cecropin-containing product can be quickly and accurately detected.
While the preferred embodiments of the present invention have been described, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.
Claims (10)
1. A cecropin-containing product characterized by: a product containing cecropin comprises cecropin and water insoluble substance, wherein the water insoluble substance comprises corncob powder.
2. The cecropin-containing product according to claim 1, characterized in that: the water-insoluble substance also comprises at least one of Maifanitum and rice hull powder.
3. A method for detecting the content of cecropin in the product of claim 1 or 2, which is characterized by comprising the following steps: comprises the following steps of (1) dissolving cecropin in a product by using a dissolving solution; (2) filtering or centrifuging to obtain solution containing cecropin; (3) and (5) measuring the content of cecropin in the solution.
4. The method for detecting cecropin content in a product according to claim 3, wherein: in the step (3), the content of cecropin in the solution is determined by using an ultraviolet spectrophotometry.
5. The method for detecting cecropin content in a product according to claim 3, wherein: in the step (3), the content of cecropin in the solution is measured by using a high performance liquid chromatograph.
6. The method for detecting cecropin content in a product according to claim 3, wherein: the dissolving solution is a mixed solution of water and an additive for reducing the tension of the water.
7. The method for detecting cecropin content in a product according to claim 6, wherein: the additive for reducing water tension is at least one of ethanol, trifluoroacetic acid and tween 80.
8. The method for detecting cecropin content in a product according to claim 7, wherein: the dissolving solution is a mixed solution of water and ethanol, and the volume fraction of the ethanol in the mixed solution is 30-60%.
9. The method for detecting cecropin content in a product according to claim 3, wherein: the dissolving process of the step (1) also comprises vortex oscillation and ultrasonic treatment.
10. The method for detecting cecropin content in a product according to claim 9, wherein: vortex and shake for 30min, and sonicate for 30 min.
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| CN202011597906.8A CN112595685A (en) | 2020-12-29 | 2020-12-29 | A product containing cecropin and method for detecting cecropin content in the product |
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