CN112586738A - Pueraria flavone buccal tablet and preparation method thereof - Google Patents
Pueraria flavone buccal tablet and preparation method thereof Download PDFInfo
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- CN112586738A CN112586738A CN202011473863.2A CN202011473863A CN112586738A CN 112586738 A CN112586738 A CN 112586738A CN 202011473863 A CN202011473863 A CN 202011473863A CN 112586738 A CN112586738 A CN 112586738A
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- pueraria
- flavone
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- 150000002212 flavone derivatives Chemical class 0.000 title claims abstract description 106
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title claims abstract description 106
- 241000219780 Pueraria Species 0.000 title claims abstract description 104
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- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims abstract description 11
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- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 12
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- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 4
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- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims description 2
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- 238000010828 elution Methods 0.000 claims description 2
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 2
- 229960002477 riboflavin Drugs 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 229960003495 thiamine Drugs 0.000 claims description 2
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- 238000005550 wet granulation Methods 0.000 description 4
- RXUWDKBZZLIASQ-UHFFFAOYSA-N Puerarin Natural products OCC1OC(Oc2c(O)cc(O)c3C(=O)C(=COc23)c4ccc(O)cc4)C(O)C(O)C1O RXUWDKBZZLIASQ-UHFFFAOYSA-N 0.000 description 3
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- HKEAFJYKMMKDOR-VPRICQMDSA-N puerarin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=CC(C2=O)=C1OC=C2C1=CC=C(O)C=C1 HKEAFJYKMMKDOR-VPRICQMDSA-N 0.000 description 3
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- 235000013361 beverage Nutrition 0.000 description 2
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- 238000001704 evaporation Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
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- 239000002994 raw material Substances 0.000 description 2
- 208000011580 syndromic disease Diseases 0.000 description 2
- 230000035922 thirst Effects 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 208000007848 Alcoholism Diseases 0.000 description 1
- 206010002383 Angina Pectoris Diseases 0.000 description 1
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- YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical compound [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 description 1
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- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
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- 208000001848 dysentery Diseases 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
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- 150000002213 flavones Chemical class 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/20—Agglomerating; Granulating; Tabletting
- A23P10/28—Tabletting; Making food bars by compression of a dry powdered mixture
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The application provides a pueraria flavone buccal tablet and a preparation method thereof, the buccal tablet comprises 30-50 parts of pueraria flavone extract, 10-15 parts of xylitol, 0.001-0.01 part of stevioside, 2.3-9.8 parts of vitamin, 3-4 parts of zinc gluconate and 10-25 parts of edible starch, the pueraria flavone extract is used as a main material and is combined with stevioside, vitamin and the like to prepare the buccal tablet to prepare a high-content pueraria flavone product, the pueraria flavone extract is combined and applied through ultrasonic countercurrent extraction, resin purification, crystallization and drying processes in sequence to obtain a pueraria extract with high flavone content through extraction, compared with a common pueraria extract extraction process, the prepared pueraria flavone buccal tablet has the advantages that the content of pueraria total flavone reaches more than 95 percent, the process is simple, the production period is short, the color of the pueraria flavone buccal tablet product is light, the prepared pueraria flavone buccal tablet has high nutrient content, and the health-care effect, convenient use, good taste, gray and light color appearance, suitability for long-term eating by middle-aged and elderly people, and good health promotion effect.
Description
Technical Field
The invention belongs to the technical field of pueraria flavone buccal tablets, and particularly relates to a pueraria flavone buccal tablet and a preparation method thereof.
Background
Pueraria lobata (Willd.) Ohwi is dry root of Pueraria lobata Ohwi of Leguminosae, and is called as Pueraria lobata Ohwi. Collected in autumn and winter, and cut into thick slices or small blocks when fresh; and (5) drying. Sweet, pungent and cool. Has the functions of expelling pathogenic factors from muscles, allaying fever, promoting eruption, promoting the production of body fluid to quench thirst, invigorating yang and stopping diarrhea. It is commonly indicated for exterior syndrome with fever, stiffness and pain of neck and back, measles without adequate eruption, thirst due to fever, diabetes due to yin deficiency, dysentery due to heat-purging and diarrhea due to spleen deficiency.
Pueraria flavone has antibacterial, blood circulation promoting, blood stasis removing, coronary artery blood vessel and cerebral vessels dilating, myocardial oxygen consumption reducing, myocardial contraction improving, blood circulation promoting, and immunity enhancing effects.
Pueraria flavonoids are mainly used for treating cardiovascular and cerebrovascular diseases, such as hypertension, hyperlipidemia, migraine, coronary heart disease, myocardial infarction, angina, retinal artery occlusion, retinal vein occlusion, sudden deafness and the like at present, and replace the traditional Chinese medicine Pueraria in Chinese patent medicines. Meanwhile, the health-care food is widely applied to the fields of health-care food (for relieving alcoholism, enlarging breast, delaying female climacteric syndrome, diabetes-induced complications and the like).
The existing pueraria flavonoid products have the conditions of low content of pueraria flavonoid and low content of effective components. The pueraria flavone buccal tablet provided by research is prepared by taking purified pueraria flavone as a raw material, screening auxiliary materials, optimizing a main formula and selecting an optimal formula process, and aims to provide a novel health-care beverage with the functions of reducing blood pressure and blood fat.
Disclosure of Invention
The invention provides a pueraria flavone buccal tablet, which is prepared by combining pueraria flavone extract with stevioside, vitamins and the like, and is presented in the form of the buccal tablet, and the mouth feel and the color are good.
Another object of the present application is to provide a preparation method of pueraria flavone buccal tablets.
A kudzuvine root flavone buccal tablet comprises the following components in percentage by weight: 30-50 parts of pueraria flavone extract, 10-15 parts of xylitol, 0.001-0.01 part of stevioside, 2.3-9.8 parts of vitamin, 3-4 parts of zinc gluconate and 10-25 parts of edible starch.
The application provides a pueraria flavone buccal tablet, which comprises 30-50 parts of pueraria flavone extract, 10-15 parts of xylitol, 0.001-0.01 part of stevioside, 2.3-9.8 parts of vitamin, 3-4 parts of zinc gluconate and 10-25 parts of edible starch, wherein the pueraria flavone extract is used as a main material and is combined with stevioside, vitamin and the like to be made into the buccal tablet, and the buccal tablet is presented in a buccal tablet form to prepare a high-content pueraria flavone product.
Preferably, the pueraria flavone extract contains more than 95% of pueraria flavone. The radix puerariae total flavone with high content is used as a raw material, and the content of the radix puerariae total flavone is measured to be more than 95 percent by adopting an ultraviolet-visible spectrophotometry, so that a novel health-care beverage with the functions of reducing blood pressure and blood fat is provided.
Preferably, the vitamins comprise 0.1-0.6 part of each of vitamin B1, vitamin B2 and vitamin E and 2-8 parts of vitamin C. Can improve the nutritive value of the product and improve the taste and flavor of the buccal tablet.
The preparation method of the pueraria flavone extract comprises the following steps:
s101, ultrasonic continuous countercurrent extraction: crushing a kudzu root medicinal material, adding a 3-5 times volume fraction of 30-60% ethanol solution, performing ultrasonic continuous countercurrent extraction at 50-80 ℃, and discharging for 1-2 hours from feeding;
s102, concentration: concentrating the extracting solution until the liquid medicine has no alcohol smell, and adding 1-3 times of hot water with volume of 60-80 ℃;
s103, resin separation: loading the concentrated solution onto macroporous resin, washing with water by 1-3 times of column volume to remove impurities, eluting with ethanol with the volume fraction of 10-45% at the temperature of 30-50 ℃, and collecting an eluent containing flavone through ultraviolet online monitoring;
s104, crystallization: concentrating the eluent containing flavone until the specific gravity of the liquid medicine is 1.1-1.2, cooling to 2-5 ℃, standing and crystallizing;
s105, drying: and drying the crystal to obtain the crystal.
The radix puerariae flavone extract is combined and applied through ultrasonic countercurrent extraction, resin purification, crystallization and drying processes in sequence, the radix puerariae extract with high flavone content is obtained through extraction, and compared with a common radix puerariae extract extraction process, the radix puerariae total flavone content prepared through the method reaches over 95 percent, and the method is simple in process, short in production period, light in product color, low in production cost, easy to realize large-scale production, and suitable for industrial mass production.
Preferably, in the step S101, the kudzuvine root is wild kudzuvine root, and is crushed to 1-2 mm. The wild kudzu root used in the application contains rich kudzu root total flavonoids, the kudzu root is crushed to 1 to 2mm by adopting a JWFC-16 universal crusher of the mechanical factory of the spring and joy of the Henan province, the kudzu root powder prepared by crushing treatment is fine and smooth, the content of the kudzu root total flavonoids contained in the kudzu root powder is high, the extraction of the high-content kudzu root total flavonoids is facilitated, and the production period is shortened.
Preferably, in step S101, the countercurrent extraction is leaching twice, and the ultrasonic frequency is 20KHz to 40 KHz. The method has the advantages that the ultrasonic countercurrent extraction efficiency is high, the operation is carried out under the whole closed condition, the loss of the pueraria flavonid in the extraction process is favorably reduced, the activity of the pueraria flavonid in the pueraria powder can be furthest kept, and the content of the pueraria flavonid is improved. Preferably, a dynamic continuous countercurrent extractor of pharmaceutical machinery factory of Jiangsu Danyang is adopted.
Preferably, in step S102 and step S104, the concentration is performed under reduced pressure, and the reduced pressure concentration is performed at 60-80 ℃ and 0.07-0.08 MPa. Its advantages are low boiling point of solution, preventing decomposition of effective substance, high evaporating efficiency and short production period.
Preferably, in step S102, the concentration is performed until the liquid medicine has no alcohol smell, specifically until the ethanol concentration is 5% or less.
Preferably, in step S103, the ultraviolet on-line monitoring uses a deuterium lamp ultraviolet detector with a wavelength of 200nm to 400 nm. The method has the advantages of high sensitivity, wide linear range and better selectivity, and is used for extracting target flavone and improving the content of the flavone, preferably with the wavelength of 220 nm.
Preferably, in step S103, the macroporous resin is selected from any one of LSA-21 macroporous resin, D101 macroporous resin, HPD-300 macroporous resin and DM-301 macroporous resin. Used for adsorbing and removing impurities.
Preferably, in step S105, the crystal is dried by spray drying under the following conditions: the air inlet temperature is 110-120 ℃, the air outlet temperature is 60-85 ℃, the feeding temperature is 60 ℃, and the feeding speed is 10 mL/min. The drying process is rapid, and is favorable for obtaining high-content pueraria flavonid.
A preparation method of pueraria flavone buccal tablets comprises the following steps:
s1, preparing the pueraria flavone extract: taking and crushing kudzuvine root, adding 30-60% of ethanol solution by volume fraction, stirring and leaching twice at 50-80 ℃, each time for 1-2 hours, merging leaching solutions, precisely filtering by adopting a plate-and-frame 300-mesh sieve, passing filtrate through macroporous resin, eluting by 10-45% of ethanol solution by volume fraction, concentrating eluent, and performing spray drying to obtain a kudzuvine root flavone extract;
s2, mixing: mixing radix Puerariae flavone extract, xylitol, stevioside, vitamins, zinc gluconate, and edible starch uniformly;
s3, tabletting: the mixture was tabletted and stored under sealed conditions. Preferably, the tabletting specifically comprises wet granulating the mixture to 50-80 meshes, drying the mixture with hot air at the temperature of 80 ℃, and tabletting under the environment with the humidity of 30-50%.
The application provides a preparation method of pueraria flavone buccal tablets, which takes pueraria flavone extracts as main materials, combines with stevioside, vitamins and the like to prepare the buccal tablets, presents in a buccal tablet form to prepare a high-content pueraria flavone product, wherein the pueraria flavone extracts are combined and applied through ultrasonic countercurrent extraction, resin purification, crystallization and drying processes in sequence, and the pueraria extract with high flavone content is obtained through extraction.
Preferably, in step S1, the kudzu root is wild kudzu root, and is pulverized to 1-2 mm. The wild kudzu root used in the application contains rich kudzu root total flavonoids, the kudzu root is crushed to 1 to 2mm by adopting a JWFC-16 universal crusher of the mechanical factory of the spring and joy of the Henan province, the kudzu root powder prepared by crushing treatment is fine and smooth, the content of the kudzu root total flavonoids contained in the kudzu root powder is high, the extraction of the high-content kudzu root total flavonoids is facilitated, and the production period is shortened.
Preferably, in step S1, a 3-5 times volume of 30-60% ethanol solution of pulverized pueraria lobata is added.
Preferably, in step S1, the leaching is ultrasonic continuous countercurrent extraction, and the ultrasonic frequency is 20KHz to 40 KHz. The method has the advantages that the ultrasonic countercurrent extraction efficiency is high, the operation is carried out under the whole closed condition, the loss of the pueraria flavonid in the extraction process is favorably reduced, the activity of the pueraria flavonid in the pueraria powder can be furthest kept, and the content of the pueraria flavonid is improved. Preferably, a dynamic continuous countercurrent extractor of pharmaceutical machinery factory of Jiangsu Danyang is adopted.
Preferably, in step S1, the "elution with an ethanol solution having a volume fraction of 10 to 45%" specifically includes: eluting with ethanol with the volume fraction of 10-45% at the temperature of 30-50 ℃, and collecting eluent containing pueraria flavone by ultraviolet online monitoring.
Preferably, in step S1, the step of "concentrating and then spray-drying the eluate" includes: concentrating the eluent until the specific gravity of the liquid medicine is 1.1-1.2, cooling to 2-5 ℃, standing for crystallization, and performing spray drying on the crystallized crystals. The concentration is carried out under reduced pressure, and the reduced pressure concentration condition is 60-80 ℃ and 0.07-0.08 Mpa. Its advantages are low boiling point of solution, preventing decomposition of effective substance, high evaporating efficiency and short production period.
Compared with the prior art, the invention has the following advantages:
the application provides a pueraria flavone buccal tablet, which comprises 30-50 parts of pueraria flavone extract, 10-15 parts of xylitol, 0.001-0.01 part of stevioside, 2.3-9.8 parts of vitamin, 3-4 parts of zinc gluconate and 10-25 parts of edible starch, wherein the pueraria flavone extract is used as a main material and is combined with stevioside, vitamin and the like to be made into the buccal tablet, and the buccal tablet is presented in a buccal tablet form to prepare a high-content pueraria flavone product.
The application provides a preparation method of pueraria flavone buccal tablets, which takes pueraria flavone extracts as main materials, combines with stevioside, vitamins and the like to prepare the buccal tablets, presents in a buccal tablet form to prepare a high-content pueraria flavone product, wherein the pueraria flavone extracts are combined and applied through ultrasonic countercurrent extraction, resin purification, crystallization and drying processes in sequence, and the pueraria extract with high flavone content is obtained through extraction.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
a preparation method of pueraria flavone buccal tablets comprises the following steps:
s1, preparing the pueraria flavone extract: taking 100kg of kudzu root medicinal material, crushing to 2mm, adding 3 times of 60% ethanol solution in volume fraction, performing ultrasonic continuous countercurrent extraction at 70 ℃, adjusting the speed from feeding to discharging for 1 hour, concentrating the extracting solution until the medicinal solution has no alcohol smell, merging leaching solutions, performing plate-frame 300-mesh precision filtration, adding 2 times of hot water in volume of the concentrated solution at 70 ℃, adding macroporous resin DM-301, washing with water for 2 times of column volume to remove impurities, eluting with 40 ℃ of 35% ethanol in volume fraction, performing ultraviolet online monitoring, collecting eluent containing flavone, concentrating the eluent at 80 ℃ until the specific gravity of the medicinal solution is 1.15, cooling to 3 ℃, standing for crystallization, and drying the crystallized crystals to obtain 6.2kg of kudzu root flavone extract;
s2, mixing: uniformly mixing 50 parts of pueraria flavone extract, 10 parts of xylitol, 0.005 part of stevioside, 3 parts of zinc gluconate, 10 parts of edible starch, 0.3 percent of each of vitamins B1, B2 and E and 5 parts of vitamin C;
s3, tabletting: and uniformly mixing the mixture, performing wet granulation to 50-80 meshes, drying by hot air at the temperature of 80 ℃, tabletting in an environment with the humidity of 30-50%, and sealing for storage.
Example 2:
a preparation method of pueraria flavone buccal tablets comprises the following steps:
s1, preparing the pueraria flavone extract: ultrasonic continuous countercurrent extraction: taking 100kg of kudzu root medicinal material, crushing to 1.5mm, adding 3 times of 60% ethanol solution in volume fraction, performing ultrasonic continuous countercurrent extraction at 80 ℃, adjusting the speed from feeding to discharging for 1.5 hours, concentrating the extracting solution until the medicinal solution has no alcohol smell, merging leaching solutions, performing plate-frame 300-mesh precision filtration, adding 2 times of hot water in volume of concentrated solution at 70 ℃, adding macroporous resin LSA-21 into the concentrated solution, washing 1 time of column volume with water to remove impurities, eluting with 30 ℃ of ethanol with volume fraction of 40%, collecting eluent containing flavone through ultraviolet online monitoring, concentrating the eluent containing flavone to specific gravity of the medicinal solution of 1.18, cooling to 4 ℃, standing for crystallization, and drying crystallized crystals to obtain 6.5kg of kudzu root flavone extract;
s2, mixing: uniformly mixing 40 parts of pueraria flavone extract, 15 parts of xylitol, 0.003 part of stevioside, 4 parts of zinc gluconate, 15 parts of edible starch, 0.4% of each of vitamins B1, B2 and E and 8 parts of vitamin C;
s3, tabletting: and uniformly mixing the mixture, performing wet granulation to 50-80 meshes, drying by hot air at the temperature of 80 ℃, tabletting in an environment with the humidity of 30-50%, and sealing for storage.
Example 3:
a preparation method of pueraria flavone buccal tablets comprises the following steps:
s1, preparing the pueraria flavone extract: ultrasonic continuous countercurrent extraction: taking 100kg of kudzu root medicinal material, crushing to 2mm, adding 5 times of 30% ethanol solution by volume fraction, carrying out ultrasonic continuous countercurrent extraction at 60 ℃, adjusting the speed from feeding to discharging for 1 hour, concentrating the extracting solution until the medicinal solution has no alcohol smell, merging leaching solutions, carrying out plate-frame 300-mesh precision filtration, adding 1 time of 80 ℃ hot water by volume of the concentrated solution, loading the concentrated solution onto macroporous resin HPD-300, washing 1 time of column volume to remove impurities, eluting with 50 ℃ of 45% ethanol by volume fraction, carrying out ultraviolet online monitoring, collecting an eluent containing flavone, concentrating the eluent containing the flavone to 1.1 of the specific gravity of the medicinal solution, cooling to 2 ℃, standing for crystallization, and drying the crystallized crystal to obtain 6.1kg of kudzu root flavone extract;
s2, mixing: uniformly mixing 30 parts of pueraria flavone extract, 13 parts of xylitol, 0.001 part of stevioside, 3.4 parts of zinc gluconate, 20 parts of edible starch, 0.1 percent of each of vitamins B1, B2 and E and 8 parts of vitamin C;
s3, tabletting: and uniformly mixing the mixture, performing wet granulation to 50-80 meshes, drying by hot air at the temperature of 80 ℃, tabletting in an environment with the humidity of 30-50%, and sealing for storage.
Example 4:
a preparation method of pueraria flavone buccal tablets comprises the following steps:
s1, preparing the pueraria flavone extract: ultrasonic continuous countercurrent extraction: taking 100kg of kudzu root medicinal material, crushing to 1mm, adding 4 times of 40% ethanol solution in volume fraction, performing ultrasonic continuous countercurrent extraction at 50 ℃, adjusting the speed from feeding to discharging for 2 hours, concentrating the extracting solution until the medicinal solution has no alcohol smell, merging leaching solutions, performing plate-frame 300-mesh precision filtration, adding 3 times of hot water at 60 ℃ in volume of the concentrated solution, passing the concentrated solution through macroporous resin DM-301, washing 1 time of column volume to remove impurities, eluting with 30 ℃ of 10% ethanol in volume fraction, performing ultraviolet online monitoring, collecting eluent containing flavone, concentrating the eluent containing flavone to 1.2 of specific gravity of the medicinal solution, cooling to 5 ℃ and standing for crystallization, and drying the crystallized crystal to obtain 6.0kg of kudzu root flavone extract;
s2, mixing: uniformly mixing 45 parts of pueraria flavone extract, 12 parts of xylitol, 0.01 part of stevioside, 3.6 parts of zinc gluconate, 25 parts of edible starch, 0.6 percent of each of vitamins B1, B2 and E and 2 parts of vitamin C;
s3, tabletting: and uniformly mixing the mixture, performing wet granulation to 50-80 meshes, drying by hot air at the temperature of 80 ℃, tabletting in an environment with the humidity of 30-50%, and sealing for storage.
The pueraria flavone extract prepared in the implementation steps 1-4 is subjected to pueraria flavone content determination, the determination method adopts an ultraviolet-visible spectrophotometry, and the determination steps are as follows:
1. accurately weighing 10mg of puerarin standard substance which is dried to constant mass, adding a proper amount of 50% ethanol with volume fraction for full dissolution, transferring to a 50m L volumetric flask, adding 50% ethanol with volume fraction for constant volume, and fully shaking up to obtain puerarin standard solution with certain concentration. And sequentially taking 5 parts of standard solutions 2, 4, 6, 8 and 10m L with equal interval volumes from the standard solutions, respectively placing the standard solutions into 5 identical 10m L volumetric flasks, and adding ethanol with the volume fraction of 50% to dilute the standard solutions to a constant volume. At the same time, the volume fraction of 50% ethanol, 1.0m L, was diluted to 10m L with distilled water as a blank, and the absorbance value was measured at 250nm with an ultraviolet-visible spectrophotometer. And drawing a standard curve by taking the concentration of the puerarin as an abscissa and the absorbance as an ordinate.
2. Weighing 50mg radix Puerariae product sample in 10m L small beaker, dissolving with 50% ethanol solution, filtering, transferring filtrate into 50ml volumetric flask, diluting with 50% ethanol to 50ml, measuring absorbance value with ultraviolet-visible spectrophotometer at 250nm, and calculating radix Puerariae flavone content according to standard curve.
The test results are shown in table 1:
TABLE 1 content test results of pueraria flavones
| Examples | Example 1 | Example 2 | Example 3 | Example 4 |
| Content of pueraria flavone | 96.3％ | 95.8％ | 96.1％ | 95.9％ |
Sensory measurement is carried out on the pueraria flavone buccal tablets prepared by implementing 1-4, and test results show that the pueraria flavone buccal tablets prepared by the method are delicious in taste, grey and light in color in appearance, suitable for long-term eating by middle-aged and elderly people, and good in health care effect.
According to test results, the content of pueraria flavone in the product prepared by the preparation method is more than 95%, and high-content pueraria flavone is obtained, the application provides a pueraria flavone buccal tablet and a preparation method thereof, pueraria flavone extract is used as a main material, combined with stevioside, vitamins and the like to prepare the buccal tablet, and presented in the form of the buccal tablet to prepare a high-content pueraria flavone product, wherein the pueraria flavone extract is combined and applied through ultrasonic countercurrent extraction, resin purification, crystallization and drying processes in sequence to extract the pueraria extract with high flavone content, compared with the common pueraria extract extraction process, the prepared pueraria flavone buccal tablet has the advantages that the content of pueraria flavone reaches more than 95%, the process is simple, the production period is short, the color of the pueraria flavone buccal tablet product is light, the prepared pueraria flavone buccal tablet has high nutrient content, and the health-care effect of pueraria, convenient use, good taste, gray and light color appearance, suitability for long-term eating by middle-aged and elderly people, and good health promotion effect.
The above description is only exemplary of the present application and should not be taken as limiting the present application, as any modification, equivalent replacement, or improvement made within the spirit and principle of the present application should be included in the protection scope of the present application.
Claims (10)
1. A kudzuvine root flavone buccal tablet is characterized in that: comprises the following components in percentage by weight: 30-50 parts of pueraria flavone extract, 10-15 parts of xylitol, 0.001-0.01 part of stevioside, 2.3-9.8 parts of vitamin, 3-4 parts of zinc gluconate and 10-25 parts of edible starch.
2. The pueraria flavonid buccal tablet according to claim 1, which is characterized in that: the content of pueraria flavone in the pueraria flavone extract is more than 95 percent.
3. The pueraria flavonid buccal tablet according to claim 1, which is characterized in that: the vitamins comprise vitamin B1, vitamin B2, 0.1-0.6 part of vitamin E and 2-8 parts of vitamin C.
4. The preparation method of the pueraria flavone buccal tablet is characterized by comprising the following steps:
s1, preparing the pueraria flavone extract: taking and crushing kudzuvine root, adding 30-60% of ethanol solution by volume fraction, stirring and leaching twice at 50-80 ℃, each time for 1-2 hours, merging leaching solutions, precisely filtering by adopting a plate-and-frame 300-mesh sieve, passing filtrate through macroporous resin, eluting by 10-45% of ethanol solution by volume fraction, concentrating eluent, and performing spray drying to obtain a kudzuvine root flavone extract;
s2, mixing: mixing radix Puerariae flavone extract, xylitol, stevioside, vitamins, zinc gluconate, and edible starch uniformly;
s3, tabletting: the mixture was tabletted and stored under sealed conditions.
5. The preparation method of pueraria flavonid buccal tablets according to claim 4, which is characterized by comprising the following steps of: in the step S3, the tabletting specifically comprises wet granulating the mixture to 50-80 mesh, drying with hot air at 80 ℃, and tabletting under an environment with humidity of 30-50%.
6. The preparation method of pueraria flavonid buccal tablets according to claim 4, which is characterized by comprising the following steps of: in step S1, the kudzu root is wild kudzu root, and is crushed to 1-2 mm.
7. The preparation method of pueraria flavonid buccal tablets according to claim 4, which is characterized by comprising the following steps of: in step S1, a 30-60% ethanol solution with a volume fraction of 3-5 times that of the pulverized radix puerariae is added.
8. The preparation method of pueraria flavonid buccal tablets according to claim 4, which is characterized by comprising the following steps of: in step S1, the leaching is ultrasonic continuous countercurrent extraction, and the ultrasonic frequency is 20 KHz-40 KHz.
9. The preparation method of pueraria flavonid buccal tablets according to claim 4, which is characterized by comprising the following steps of: in step S1, the "elution with 10-45% by volume of ethanol solution" specifically includes: eluting with ethanol with the volume fraction of 10-45% at the temperature of 30-50 ℃, and collecting eluent containing pueraria flavone by ultraviolet online monitoring.
10. The preparation method of pueraria flavonid buccal tablets according to claim 4, which is characterized by comprising the following steps of: in step S1, the step of concentrating the eluate and then spray-drying includes: concentrating the eluent until the specific gravity of the liquid medicine is 1.1-1.2, cooling to 2-5 ℃, standing for crystallization, and performing spray drying on the crystallized crystals.
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