CN112574931B - Biocontrol microbial inoculum for preventing and treating garlic diseases as well as preparation method and application thereof - Google Patents
Biocontrol microbial inoculum for preventing and treating garlic diseases as well as preparation method and application thereof Download PDFInfo
- Publication number
- CN112574931B CN112574931B CN202110078784.XA CN202110078784A CN112574931B CN 112574931 B CN112574931 B CN 112574931B CN 202110078784 A CN202110078784 A CN 202110078784A CN 112574931 B CN112574931 B CN 112574931B
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- China
- Prior art keywords
- microbial inoculum
- garlic
- pseudomonas aeruginosa
- biocontrol microbial
- biocontrol
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 235000004611 garlic Nutrition 0.000 title claims abstract description 64
- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 49
- 230000000443 biocontrol Effects 0.000 title claims abstract description 40
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- 201000010099 disease Diseases 0.000 title abstract description 21
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title abstract description 21
- 244000245420 ail Species 0.000 title 1
- 240000002234 Allium sativum Species 0.000 claims abstract description 63
- 238000000855 fermentation Methods 0.000 claims abstract description 35
- 230000004151 fermentation Effects 0.000 claims abstract description 35
- 244000052616 bacterial pathogen Species 0.000 claims abstract description 29
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims abstract description 23
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 20
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 20
- 244000053095 fungal pathogen Species 0.000 claims abstract description 13
- 239000002689 soil Substances 0.000 claims abstract description 13
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- 239000002131 composite material Substances 0.000 claims abstract description 3
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- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 6
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
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- 229960004793 sucrose Drugs 0.000 claims description 3
- 239000004480 active ingredient Substances 0.000 claims description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- 230000002401 inhibitory effect Effects 0.000 claims description 2
- 235000013379 molasses Nutrition 0.000 claims description 2
- 239000003755 preservative agent Substances 0.000 claims description 2
- 230000002335 preservative effect Effects 0.000 claims description 2
- 239000012681 biocontrol agent Substances 0.000 claims 2
- 230000012010 growth Effects 0.000 abstract description 5
- 230000003385 bacteriostatic effect Effects 0.000 description 8
- 230000000844 anti-bacterial effect Effects 0.000 description 7
- 230000005764 inhibitory process Effects 0.000 description 6
- 238000012258 culturing Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 230000002265 prevention Effects 0.000 description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 4
- 229910021641 deionized water Inorganic materials 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 244000223014 Syzygium aromaticum Species 0.000 description 3
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- 244000000005 bacterial plant pathogen Species 0.000 description 3
- 239000003899 bactericide agent Substances 0.000 description 3
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- 238000007865 diluting Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000001965 potato dextrose agar Substances 0.000 description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 239000007836 KH2PO4 Substances 0.000 description 2
- 239000012880 LB liquid culture medium Substances 0.000 description 2
- 239000006142 Luria-Bertani Agar Substances 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 2
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000007790 scraping Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 239000004278 EU approved seasoning Substances 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000233614 Phytophthora Species 0.000 description 1
- 206010037549 Purpura Diseases 0.000 description 1
- 241001672981 Purpura Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000221662 Sclerotinia Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
- 239000003876 biosurfactant Substances 0.000 description 1
- 238000003967 crop rotation Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 244000000004 fungal plant pathogen Species 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005360 mashing Methods 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002881 soil fertilizer Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/27—Pseudomonas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Environmental Sciences (AREA)
- Virology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Soil Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202110078784.XA CN112574931B (en) | 2021-01-21 | 2021-01-21 | Biocontrol microbial inoculum for preventing and treating garlic diseases as well as preparation method and application thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN202110078784.XA CN112574931B (en) | 2021-01-21 | 2021-01-21 | Biocontrol microbial inoculum for preventing and treating garlic diseases as well as preparation method and application thereof |
Publications (2)
Publication Number | Publication Date |
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CN112574931A CN112574931A (en) | 2021-03-30 |
CN112574931B true CN112574931B (en) | 2022-04-22 |
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CN202110078784.XA Active CN112574931B (en) | 2021-01-21 | 2021-01-21 | Biocontrol microbial inoculum for preventing and treating garlic diseases as well as preparation method and application thereof |
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CN (1) | CN112574931B (en) |
Families Citing this family (1)
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CN115369138A (en) * | 2022-09-07 | 2022-11-22 | 曲阜师范大学 | Method for preparing rhamnolipid by solid-state fermentation of peanut cakes |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102154186B (en) * | 2011-04-14 | 2012-09-05 | 中国农业科学院烟草研究所 | Bacillus subtilis and use thereof in prevention and control of fungus disease |
CN103952328B (en) * | 2013-11-13 | 2016-08-17 | 陕西省微生物研究所 | A kind of bacillus subtilis strain A33, microbial inoculum and preparation method and application |
CN111575199B (en) * | 2020-04-21 | 2022-02-11 | 华南农业大学 | Pseudomonas aeruginosa JT86 and application thereof in preventing and treating sclerotinia rot |
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2021
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Effective date of registration: 20240909 Address after: 750205, 300 meters northeast of the intersection of Changtai Road and Hongyun East Road in Hongguang Town, Helan Industrial Park, Helan County, Yinchuan City, Ningxia Hui Autonomous Region Patentee after: Ningxia Wodidi Water and Fertilizer Technology Co.,Ltd. Country or region after: China Address before: No. 1104, 11th Floor, Unit 1, Building 1, Eurasia Scenic Area, Xi'an International Port Zone, Shaanxi Province, 710026 Patentee before: Xi'an Huaqi Zhongxin Technology Development Co.,Ltd. Country or region before: China Effective date of registration: 20240906 Address after: No. 1104, 11th Floor, Unit 1, Building 1, Eurasia Scenic Area, Xi'an International Port Zone, Shaanxi Province, 710026 Patentee after: Xi'an Huaqi Zhongxin Technology Development Co.,Ltd. Country or region after: China Address before: 273165 Jingxuan West Road, Qufu City, Jining, Shandong Province, No. 57 Patentee before: QUFU NORMAL University Country or region before: China |
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TR01 | Transfer of patent right |