CN112574910B - Pymetrozine degrading bacterium RJJ-1 and application thereof - Google Patents
Pymetrozine degrading bacterium RJJ-1 and application thereof Download PDFInfo
- Publication number
- CN112574910B CN112574910B CN202011455791.9A CN202011455791A CN112574910B CN 112574910 B CN112574910 B CN 112574910B CN 202011455791 A CN202011455791 A CN 202011455791A CN 112574910 B CN112574910 B CN 112574910B
- Authority
- CN
- China
- Prior art keywords
- pymetrozine
- rjj
- degrading bacteria
- degrading
- degradation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/306—Pesticides
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention discloses a pymetrozine degrading bacterium RJJ-1 and application thereof, belongs to the field of biotechnology, and experiments prove that the pymetrozine degrading bacterium RJJ-1 can survive in an environment with pymetrozine as a unique carbon source and has high pymetrozine degrading activity.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to pymetrozine degrading bacteria RJJ-1 and application thereof.
Background
With the increase of population pressure, the global demand for food is gradually increased, and the pesticide and chemical fertilizer is favored by people as an agricultural means for effectively reducing crop loss and improving the quality of agricultural products, but meanwhile, pesticide residue pollution also brings serious harm to ecological environment and human health. Pymetrozine is a pyridinylmethylamine insecticide, and is widely used for controlling pests such as trialeurodes vaporariorum, leafhopper, cotton aphid and the like in crops due to excellent insecticidal effect. However, unreasonable and overdose use of chemical pesticides in the process of preventing and treating crop diseases and insect pests by people leads to higher and higher pesticide residue in agricultural products. Today, three decades after pymetrozine appears, residual pymetrozine can be detected in living environment, agricultural products and bee bodies, but the residual quantity is greatly different due to individual difference. It has been reported that pymetrozine is highly toxic to aquatic organisms and has both genotoxic and weakly cytotoxic effects on human peripheral blood lymphocytes. The report of the Joint FAO/WHO Meeting on Pesticide Residues for short JMPR of grain and agriculture organization/health organization shows that pymetrozine can be identified as potentially harmful to fetuses, babies and children; pymetrozine has been classified as a possible human carcinogen by the U.S. environmental Protection Agency (EPA for short). Therefore, the environmental behavior and degradation mechanism of pymetrozine are attracting much attention.
The microbial degradation is characterized by high efficiency, low price, safety, good effect on restoring the ecological balance and the productivity of agricultural soil and the like, and becomes an effective measure for treating various organic pollutants. In recent years, the degradation of pesticide residue contamination by microorganisms has attracted much attention. After extensive research, the researchers have isolated a large number of microbial groups capable of degrading or transforming one or more pesticides. At present, many developed countries and large companies invest huge capital to research and develop bacteria capable of degrading pesticide residues efficiently. Therefore, the separation and screening of microorganisms having efficient degradation characteristics from the natural world and the research of their degradation mechanism have become the hot spots of research today.
Disclosure of Invention
The invention aims to provide pymetrozine degrading bacteria RJJ-1 and application thereof in the aspects of pymetrozine degradation in soil and water and the like.
In order to solve the technical problems, the invention provides the following technical scheme: pymetrozine degrading bacteria RJJ-1; wherein the pymetrozine-degrading bacteria RJJ-1 are preserved in the common microorganism center of China Committee for culture Collection of microorganisms 23.12.2019, the preservation unit address is No. 3 of Xilu No. 1 of Beijing market facing Yang area, the preservation number is CGMCC No. 19234, and the classification is named as Pseudomonas aeruginosa (Pseudomonas aeruginosa).
The pymetrozine-degrading bacterium RJJ-1 has a repairing effect on an environment polluted by pymetrozine, and particularly has a repairing effect on a soil environment polluted by pymetrozine and a water body polluted by pymetrozine.
The pymetrozine degrading bacteria RJJ-1 are concerned to degrade the waste pymetrozine in the soil and the pymetrozine in the water, and can be directly applied to the field of purification treatment of farmlands.
The screening method of the pymetrozine-degrading bacteria RJJ-1 comprises the following steps:
1) Weighing soil from sludge polluted by pymetrozine, adding pymetrozine-degrading bacteria RJJ-1 screening liquid culture medium for enrichment, and culturing in a constant temperature shaking table at 35-40 deg.C and 150-200r/min for 4-10 days;
2) Absorbing the culture medium obtained in the step 1, adding a new pymetrozine degradation bacterium RJJ-1 screening liquid culture medium for passage, and carrying out passage for 3-6 times;
3) Scribing and separating the bacterial liquid obtained in the step (2) on an LB (LB) flat plate to obtain pymetrozine degrading bacteria RJJ-1;
wherein the pymetrozine-degrading bacteria RJJ-1 screening culture medium comprises the following components: each 1L of deionized water contains 100mg/L of pymetrozine pesticide, 0.66g of ammonium chloride and dipotassium hydrogen phosphate (K) 2 HPO 4 ) 1.5g of potassium dihydrogen phosphate (KH) 2 PO 4 ) 0.5g magnesium sulfate (MgSO) 4 ) 0.1g of dipotassium hydrogen phosphate (K) 2 HPO 4 ) 1.5g, 0.35g of sodium chloride, pH7.0-7.2;
the LB solid medium comprises the following components: 10g of peptone, 5g of yeast powder, 10g of sodium chloride and 15g of agar powder, and the volume is made up to 1L by deionized water.
The preferable screening method of the pymetrozine-degrading bacteria RJJ-1 comprises the following steps:
1) Weighing 5g of soil from sludge polluted by pymetrozine, adding 100mL of pymetrozine degrading bacteria RJJ-1 screening liquid culture medium for enrichment, and culturing in a constant temperature shaking table at 37 ℃ and 180r/min for 5-7 days;
2) 3mL of the culture medium obtained in the step 1 is absorbed and added with a new pymetrozine degradation bacterium RJJ-1 screening liquid culture medium for passage, and the passage is carried out for 4 to 5 times;
3) Scribing and separating the bacterial liquid obtained in the step 2 on an LB (Langmuir-Blodgett) plate to obtain pymetrozine degrading bacteria RJJ-1;
the dry powder of the pymetrozine-degrading bacteria RJJ-1 is prepared by drying the pymetrozine-degrading bacteria RJJ-1 subjected to amplification culture by a conventional method.
The pymetrozine degrading bacteria RJJ-1 are obtained by screening according to the following method:
1. soil separation strain is weighed from sludge polluted by pymetrozine in Changzhou Wucheng area of Jiangsu
Weighing 5g of sludge, adding 95mL of pymetrozine degrading bacteria RJJ-1 screening liquid culture medium for enrichment, and culturing for 5-7 days in a constant-temperature shaking table at 37 ℃ and 180 r/min; then 3mL of culture medium is sucked and added with a new pymetrozine degradation bacterium RJJ-1 screening liquid culture medium for passage, the passage is carried out for 4-5 times, streaking separation is carried out on an LB (Langmuir-Blodgett) plate, and a batch of strains which can survive in the culture medium taking pymetrozine as a unique carbon source are finally obtained through multiple separation test screening;
the pymetrozine degrading bacteria RJJ-1 screening culture medium comprises the following components: each 1L of deionized water contains 100mg/L of pymetrozine pesticide, 0.66g of ammonium chloride and dipotassium hydrogen phosphate (K) 2 HPO 4 ) 1.5g, monopotassium phosphate (KH) 2 PO 4 ) 0.5g magnesium sulfate (MgSO) 4 ) 0.1g of dipotassium hydrogen phosphate (K) 2 HPO 4 ) 1.5g, 0.35g of sodium chloride, pH7.0-7.2;
the LB solid medium comprises the following components: 10g of peptone, 5g of yeast powder, 10g of sodium chloride and 15g of agar powder, and deionized water is used for complementing the volume to 1L.
2. Analyzing the degradation efficiency of the different strains obtained by separation on the pymetrozine in the culture medium, and screening to obtain the strain with the best pymetrozine degradation effect.
The specific determination method is as follows:
1. preparation of the Medium
Basic salt culture medium: NH (NH) 4 Cl0.66g，K 2 HPO 4 1.5g，KH 2 PO 4 0.5g，MgSO 4 0.1g，K 2 HPO 4 1.5g, naCl0.35g, pH7.0-7.2; sterilizing at 121 deg.C for 20min.
LB liquid medium: 5g of yeast powder, 10g of sodium chloride, 10g of peptone and 1000mL of deionized water, wherein the components are sterilized at 121 ℃ for 20min.
2. Method for measuring pymetrozine degradation efficiency
2.1 drawing of Standard Curve for pymetrozine
Taking 0.05g of pymetrozine standard substance, dissolving the standard substance by using chromatographic acetonitrile, and carrying out constant volume to a 50mL volumetric flask to prepare 1000mg/L mother liquor. Diluting the mother liquor with chromatographic pure acetonitrile, preparing 10, 20, 40, 50, 60, 80 and 100mg/L series standard solutions step by step, and measuring peak area by using a high performance liquid chromatograph under the condition of a wavelength of 298nm to obtain a concentration-peak area standard curve.
2.2 method for measuring degradation rate of pymetrozine
The culture solution was taken, and eluted by high performance liquid chromatography at 298nm with a mobile phase of a mixture containing methanol/water/formic acid (100, 900, v/v/v) at a flow rate of 1.0 mL/min. The injection volume was 20. Mu.L, the column temperature was kept at 40 ℃ and the peak area was determined. And (4) calculating the pymetrozine concentration by using a linear regression equation established by a pymetrozine standard curve, and further calculating to obtain the pymetrozine degradation rate. Three replicates were set up for all experiments.
2.3 obtaining the optimal strain through the degradation rate of the pymetrozine by pymetrozine degrading bacteria RJJ-1
Culturing pymetrozine-degrading bacteria RJJ-1 strain in LB liquid culture medium, centrifugally collecting thallus, re-suspending with sterile water, and diluting to OD 600 =1, inoculating the selected strain to 1-100.0 mL of a basic liquid inorganic salt culture medium containing 100mg/L pymetrozine, placing the culture medium in a constant temperature shaking table for 180r/min, culturing at 37 ℃, and sampling every 6 hours to determine the degradation rate.
The screened pymetrozine-degrading bacteria RJJ-1 have obvious effect on the degradation of pymetrozine, the strain grows well, and the degradation rate of pymetrozine for 30 hours is 86.34%.
2.4 degradation of waste pymetrozine in soil by pymetrozine degradation bacteria RJJ-1
Inoculating pymetrozine-degrading bacteria RJJ-1 into 100mL of sterilized wastewater containing pymetrozine according to the inoculation amount of 1%, determining the content of pymetrozine in the wastewater to be 55mg/L by a high performance liquid chromatograph, culturing for 30h under the conditions of 37 ℃ and 180r/min, and determining the pymetrozine degradation rate as follows: 79.33 percent.
2.5 degradation of waste pymetrozine in wastewater by pymetrozine degradation bacteria RJJ-1
Inoculating pymetrozine-degrading bacteria RJJ-1 into 100mL of sterilized soil solution according to the inoculation amount of 1%, determining by a high performance liquid chromatograph that the content of pymetrozine in the soil is 60mg/Kg, culturing for 30h under the constant temperature condition of 37 ℃, and determining that the degradation rate of pymetrozine is 75.67%.
Identification of strains
Obtaining genome DNA of pymetrozine degrading bacteria RJJ-1 by adopting an oscillation crushing method, amplifying 16S rRNA genes of the strains by a PCR method, and sending the amplified genes to Shanghai Jieli biological company for sequencing analysis; the upstream primer sequences used to amplify the 16S rRNA gene were: 5 'AGAGAGTTTGATCCTGGCTCAG 3' and the downstream primer sequence is 5'GGTTACCTTGTTACGACTT 3'.
The PCR reaction conditions were: pre-denaturation at 98 ℃ for 3min, denaturation at 98 ℃ for 15s, annealing at 56 ℃ for 15s, extension at 72 ℃ for 40s, reaction for 31 cycles, and extension at 10 ℃ for 5min. Using the BLAST function of NCBI for sequence alignment in the GeneBank database, the classification of the analyzed strains was: pseudomonas aeruginosa (Pseudomonas aeruginosa)
The gene sequence of the pymetrozine degrading bacteria RJJ-1 1696 rRNA is shown in a sequence table.
The beneficial effects of the invention are as follows:
1. a pymetrozine degrading bacterium RJJ-1 which can survive in an environment with pymetrozine as a unique carbon source is screened out, and degradation analysis shows that the strain can grow well in a solid culture medium and a liquid culture medium containing 100mg/L of pymetrozine, and has high pymetrozine degrading characteristics.
2. The pymetrozine-degrading bacteria RJJ-1 have application prospects in the degradation of pymetrozine in soil, the degradation of pymetrozine in water body pollution, the degradation of waste pymetrozine in soil and the restoration of environmental pollution.
3. The application of the pymetrozine-degrading bacteria RJJ-1 is beneficial to avoiding secondary pollution caused by physical or chemical method degradation of pymetrozine.
Drawings
FIG. 1 is a morphological diagram of a single colony of the pymetrozine-degrading bacterium RJJ-1.
FIG. 2 shows the pymetrozine degradation rate measured by inoculating pymetrozine-degrading bacteria RJJ-1 at an inoculum size of 1% into 100mL of sterile MSM.
FIG. 3 shows the pymetrozine degradation rate measured by inoculating pymetrozine-degrading bacteria RJJ-1 in 100mL of pymetrozine-containing wastewater according to the inoculation amount of 1%.
FIG. 4 shows the pymetrozine degradation rate measured by inoculating 1% of pymetrozine-degrading bacteria RJJ-1 into 100g of sterilized soil.
Detailed Description
Screening process of pymetrozine degrading bacteria RJJ-1
(I) main material
1. Culture medium
Pymetrozine screening liquid medium components: 1L of deionized water contains pymetrozine100mg/L of ketone pesticide, 0.66g of ammonium chloride and dipotassium hydrogen phosphate (K) 2 HPO 4 ) 1.5g of potassium dihydrogen phosphate (KH) 2 PO 4 ) 0.5g magnesium sulfate (MgSO) 4 ) 0.1g of dipotassium hydrogen phosphate (K) 2 HPO 4 ) 1.5g, 0.35g of sodium chloride, pH7.0-7.2;
composition of LB liquid Medium per 1L: 10g of peptone, 5g of yeast powder and 10g of NaCl are respectively added, the pH of the prepared liquid culture medium is adjusted to 7.0-7.2 by HCl or NaOH, and then deionized water is added to make up to 1L.
The corresponding solid culture medium is prepared by adding 15g of agar powder into 1L of liquid culture medium.
2. Instrumentation and equipment
The system comprises a Bio-Rad PCR amplification instrument, a Tanon-3500 gel imaging system, an electrophoresis apparatus of six instruments factory in Beijing, a sterilization pot, an electronic balance, a UV1900 ultraviolet visible spectrophotometer, a constant temperature incubator, an eppendorf high-speed refrigerated centrifuge, a shaking table and a low-temperature refrigerator of Qingdao Haier group.
(II) screening process of pymetrozine-degrading bacteria RJJ-1
(1) Weighing an earth separation strain from sludge polluted by pymetrozine in Wuji area of Jiangsu province, weighing 5g of sludge, adding 100mL of pymetrozine degrading bacteria RJJ-1 screening liquid culture medium for enrichment, and culturing for 5-7 days in a constant-temperature shaking table at 37 ℃ and 180 r/min; then 3mL of culture medium is sucked and added with a new pymetrozine degradation bacterium RJJ-1 screening liquid culture medium for passage, the passage is carried out for 4-5 times, streaking separation is carried out on an LB (Langmuir-Blodgett) plate, and a batch of strains which can survive in the culture medium taking pymetrozine as a unique carbon source are finally obtained through multiple separation test screening;
the pymetrozine degrading bacteria RJJ-1 screening culture medium comprises the following components: each 1L of deionized water contains 100mg/L of pymetrozine pesticide, 0.66g of ammonium chloride and dipotassium hydrogen phosphate (K) 2 HPO 4 ) 1.5g, monopotassium phosphate (KH) 2 PO 4 ) 0.5g magnesium sulfate (MgSO) 4 ) 0.1g of dipotassium hydrogenphosphate (K) 2 HPO 4 ) 1.5g, 0.35g of sodium chloride, pH7.0-7.2;
the LB solid medium comprises the following components: 10g of peptone, 5g of yeast powder, 10g of sodium chloride and 15g of agar powder, and deionized water is used for complementing the volume to 1L.
(2) Streaking and separating the culture on an LB plate, and culturing in a constant-temperature incubator at 37 ℃ for 12-26 hours to obtain a morphological map of a single colony of the pymetrozine-degrading strain RJJ-1 (see figure 1).
The following is a specific example section:
example 1
Culturing pymetrozine-degrading bacteria RJJ-1 in LB liquid culture medium, centrifugally collecting bacteria, re-suspending with sterile water, and diluting to OD 600 =1, inoculating 1mL of the selected strain into a basic liquid inorganic salt culture medium containing 100.0mg/L of pymetrozine, placing the strain in a constant temperature shaking table for culturing for 30h at 37 ℃ at 180r/min, wherein pymetrozine degradation bacteria RJJ-1 have obvious growth and higher pymetrozine degradation efficiency.
Example 2
The pymetrozine-degrading bacteria RJJ-1 are inoculated into 100mL of sterile MSM according to the inoculation amount of 1%, pymetrozine is added to the final concentration of 100mg/L, the mixture is cultured for 30h under the conditions of 37 ℃ and 180r/min, and the pymetrozine degradation rate is measured to be 86.34% (see figure 2).
Example 3
Inoculating pymetrozine-degrading bacteria RJJ-1 into 100mL of waste water containing pymetrozine according to the inoculation amount of 1%, determining that the content of pymetrozine in the waste water is 60mg/L, culturing for 30h under the conditions of 37 ℃ and 180r/min, and determining that the pymetrozine degradation rate is as follows: 79.33% (see fig. 3).
Example 4
The pymetrozine-degrading bacteria RJJ-1 are inoculated into 100g of sterilized soil according to the inoculation amount of 1%, the content of pymetrozine in the soil is determined to be 55mg/Kg, the soil is cultured for 30h under the constant temperature condition of 37 ℃, and the pymetrozine degradation rate is determined to be 75.67% (see figure 4).
Example 5
The preparation method comprises the steps of preparing a dry powder of the pymetrozine degradation bacteria RJJ-1, carrying out amplification culture on the pymetrozine degradation bacteria RJJ-1, and drying by a conventional method.
Sequence listing
<110> university of Changzhou
HEIBEI CIXIN ENVIRONMENTAL PROTECTION AND TECHNOLOGY Co.,Ltd.
Jiangsu bio Environmental Protection Technology Co.,Ltd.
<120> pymetrozine degrading bacterium RJJ-1 and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1418
<212> DNA
<213> Pseudomonas aeruginosa (Pseudomonas aeruginosa)
<400> 1
cgcggctacc atgcagtcga gcggatgaag ggagcttgct cctggattca gcggcggacg 60
ggtgagtaat gcctaggaat ctgcctggta gtgggggata acgtccggaa acgggcgcta 120
ataccgcata cgtcctgagg gagaaagtgg gggatcttcg gacctcacgc tatcagatga 180
gcctaggtcg gattagctag ttggtggggt aaaggcctac caaggcgacg atccgtaact 240
ggtctgagag gatgatcagt cacactggaa ctgagacacg gtccagactc ctacgggagg 300
cagcagtggg gaatattgga caatgggcga aagcctgatc cagccatgcc gcgtgtgtga 360
agaaggtctt cggattgtaa agcactttaa gttgggagga agggcagtaa gttaatacct 420
tgctgttttg acgttaccaa cagaataagc accggctaac ttcgtgccag cagccgcggt 480
aatacgaagg gtgcaagcgt taatcggaat tactgggcgt aaagcgcgcg taggtggttc 540
agcaagttgg atgtgaaatc cccgggctca acctgggaac tgcatccaaa actactgagc 600
tagagtacgg tagagggtgg tggaatttcc tgtgtagcgg tgaaatgcgt agatatagga 660
aggaacacca gtggcgaagg cgaccacctg gactgatact gacactgagg tgcgaaagcg 720
tggggagcaa acaggattag ataccctggt agtccacgcc gtaaacgatg tcgactagcc 780
gttgggatcc ttgagatctt agtggcgcag ctaacgcgat aagtcgaccg cctggggagt 840
acggccgcaa ggttaaaact caaatgaatt gacgggggcc cgcacaagcg gtggagcatg 900
tggtttaatt cgaagcaacg cgaagaacct tacctggcct tgacatgctg agaactttcc 960
agagatggat tggtgccttc gggaactcag acacaggtgc tgcatggctg tcgtcagctc 1020
gtgtcgtgag atgttgggtt aagtcccgta acgagcgcaa cccttgtcct tagttaccag 1080
cacctcgggt gggcactcta aggagactgc cggtgacaaa ccggaggaag gtggggatga 1140
cgtcaagtca tcatggccct tacggccagg gctacacacg tgctacaatg gtcggtacaa 1200
agggttgcca agccgcgagg tggagctaat cccataaaac cgatcgtagt ccggatcgca 1260
gtctgcaact cgactgcgtg aagtcggaat cgctagtaat cgtgaatcag aatgtcacgg 1320
tgaatacgtt cccgggcctt gtacacaccg cccgtcacac catgggagtg ggttgctcca 1380
gaagtagcta gtctaaccgc aagggggacg gtaccacg 1418
Claims (5)
1. The application of the pymetrozine-degrading bacteria RJJ-1 is characterized in that the pymetrozine-degrading bacteria RJJ-1 is classified and named as pseudomonas aeruginosa (Pseudomonas aeruginosa)Pseudomonas aeruginosa) The pymetrozine-degrading bacterium RJJ-1 is preserved in the China general microbiological culture Collection center of China Committee for culture Collection of microorganisms in 2019, 12 and 23 months, and the preservation number is CGMCC No. 19234;
the pymetrozine-degrading bacteria RJJ-1 have a repairing effect on the environment polluted by pymetrozine.
2. The application of the pymetrozine-degrading bacteria RJJ-1 as claimed in claim 1, wherein said pymetrozine-degrading bacteria RJJ-1 has a repairing effect on environment polluted by waste pymetrozine in soil.
3. The application of the pymetrozine-degrading bacteria RJJ-1 as claimed in claim 1, wherein the pymetrozine-degrading bacteria RJJ-1 has a repairing effect on water environment polluted by pymetrozine in water body.
4. The use of pymetrozine-degrading bacteria RJJ-1 as claimed in claim 1, wherein said pymetrozine-degrading bacteria RJJ-1 has a degrading effect on waste pymetrozine in soil.
5. The use of the pymetrozine-degrading bacteria RJJ-1 as claimed in claim 1, wherein said pymetrozine-degrading bacteria RJJ-1 has a degrading effect on pymetrozine contained in a water body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011455791.9A CN112574910B (en) | 2020-12-10 | 2020-12-10 | Pymetrozine degrading bacterium RJJ-1 and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011455791.9A CN112574910B (en) | 2020-12-10 | 2020-12-10 | Pymetrozine degrading bacterium RJJ-1 and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112574910A CN112574910A (en) | 2021-03-30 |
| CN112574910B true CN112574910B (en) | 2023-03-31 |
Family
ID=75131445
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011455791.9A Active CN112574910B (en) | 2020-12-10 | 2020-12-10 | Pymetrozine degrading bacterium RJJ-1 and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112574910B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101096644A (en) * | 2006-06-26 | 2008-01-02 | 谢明 | Pseudomonas stutzeri JSD-008 and its degradation function for organophosphorus pesticide |
| CN111893052A (en) * | 2020-05-25 | 2020-11-06 | 常州大学 | Erythromycin-degrading bacterium pseudomonas aeruginosa RJJ-1 and application thereof |
-
2020
- 2020-12-10 CN CN202011455791.9A patent/CN112574910B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101096644A (en) * | 2006-06-26 | 2008-01-02 | 谢明 | Pseudomonas stutzeri JSD-008 and its degradation function for organophosphorus pesticide |
| CN111893052A (en) * | 2020-05-25 | 2020-11-06 | 常州大学 | Erythromycin-degrading bacterium pseudomonas aeruginosa RJJ-1 and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 特丁噻草隆降解菌的分离鉴定及降解特性;任建军等;《江苏农业学报》;20200430(第02期);第276-278页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112574910A (en) | 2021-03-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102757915B (en) | Chloro acetamide herbicide degrading bacteria as well as bactericide prepared thereby and application thereof | |
| CN109468243B (en) | Bacillus siamensis and application thereof | |
| CN111154688A (en) | Biocontrol bacillus beleisi SF259 and application thereof | |
| CN101338284B (en) | Degradation strain capable of high-efficiency degrading bactericide chlorothalonil and use thereof | |
| CN105602872B (en) | The bacterium of one high-efficiency degradation glyphosate | |
| CN112481170B (en) | Pymetrozine degrading bacterium IURM F18 and application thereof | |
| CN110283759B (en) | Separation and application of grease degrading bacterium Kosakonia cowaniiuMR B67 | |
| CN110283757B (en) | Grease degrading bacterium IUMR B53 and application thereof | |
| CN112574910B (en) | Pymetrozine degrading bacterium RJJ-1 and application thereof | |
| CN111334457A (en) | Bacillus acquired immaturus with soil heavy metal restoration effect and application thereof | |
| CN1302865A (en) | Bacterium for degradating residual agricultural organophosphorus chemical and its bacterial preparation | |
| CN103160447A (en) | Degrading bacteria capable of efficiently degrading pesticide chlorothalonil, and application thereof | |
| CN110157637A (en) | Enterobacteria Z1 and klebsiella Z2 composite bacteria agent removal high nitrogen pollutant effluents and application | |
| CN112391312B (en) | Streptomyces and application thereof in prevention and treatment of plant oomycete diseases | |
| CN112375718B (en) | Pymetrozine degrading bacterium IURM F17 and application thereof | |
| CN112501067B (en) | Pymetrozine degrading bacterium IURM B56 and application thereof | |
| CN112592848B (en) | Pymetrozine degrading bacterium IUMR B53 and application thereof | |
| CN102827799B (en) | Bacteria for degrading herbicide cyhalofop-butyl and application thereof | |
| CN112877243B (en) | Darfeld lakeda bacterium for degrading acephate and application thereof | |
| CN114717144B (en) | Achromobacter strain, application thereof and soil improvement microbial inoculum containing same | |
| CN114058557B (en) | Multifunctional bio-control degradation strain of bacillus megatherium and application thereof | |
| CN114437998B (en) | Microbacterium rubrum for promoting growth of rice and improving cold resistance of seedlings and application thereof | |
| CN110577908B (en) | Degradation strain of pyrethroid insecticide and application thereof | |
| Dilys | Isolation, Identification and Characterization of Bacteria Capable of Degrading Chlorpyrifos from Agricultural Soil at Amansea, Anambra State Nigeria. Emeribe, Chiemeka Elochi, Onuorah, Samuel Chinedu and Chukwukelo | |
| CN116656560A (en) | Micrococcus yunnanensis HY001 strain and application thereof in degradation of paclobutrazol |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |