CN112557317A - Method for measuring zinc ion content - Google Patents
Method for measuring zinc ion content Download PDFInfo
- Publication number
- CN112557317A CN112557317A CN202011314693.3A CN202011314693A CN112557317A CN 112557317 A CN112557317 A CN 112557317A CN 202011314693 A CN202011314693 A CN 202011314693A CN 112557317 A CN112557317 A CN 112557317A
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- CN
- China
- Prior art keywords
- measuring
- content
- zinc
- zinc ion
- buffer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 17
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims abstract description 14
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 13
- 239000000872 buffer Substances 0.000 claims abstract description 12
- 238000002798 spectrophotometry method Methods 0.000 claims abstract description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 7
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000007864 aqueous solution Substances 0.000 claims abstract description 7
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000004327 boric acid Substances 0.000 claims abstract description 7
- 239000001103 potassium chloride Substances 0.000 claims abstract description 7
- 235000011164 potassium chloride Nutrition 0.000 claims abstract description 7
- 239000011701 zinc Substances 0.000 claims abstract description 7
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 7
- 239000000126 substance Substances 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 5
- 239000012086 standard solution Substances 0.000 claims description 5
- 239000012482 calibration solution Substances 0.000 description 6
- 238000005259 measurement Methods 0.000 description 3
- 239000006172 buffering agent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000010421 standard material Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/1813—Specific cations in water, e.g. heavy metals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/775—Indicator and selective membrane
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pathology (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention provides a method for measuring zinc ion content, which is used for measuring the zinc ion content by a spectrophotometry and comprises the following steps: (1) adding 7mL of standard sample (or water sample) into a 10mL colorimetric tube, adding 2mL of buffer, and uniformly mixing; (2) then adding 1mL of color developing agent, and uniformly mixing; (3) calibrating or measuring by spectrophotometry at the wavelength of 620nm after 10 min; wherein, the buffer agent is that each liter of aqueous solution contains 2g to 10g of boric acid, 1g to 6g of potassium chloride and 0.1g to 5g of sodium hydroxide. The color developing agent is 0.1-5 g of zinc reagent and 250-980 mL of absolute ethyl alcohol in each liter of aqueous solution.
Description
Technical Field
The invention relates to a method for measuring zinc ion content, which is used for measuring the zinc ion content by a spectrophotometry.
Background
The determination of the zinc ion content is important and frequent in many departments of life science field, chemical industry, environmental protection, medical treatment, energy and the like, and has wide requirements.
In the prior art, the spectrophotometry is adopted to measure the content of zinc ions, the method is generally carried out in a laboratory, a plurality of volumetric flasks are adopted to prepare solution and treatment reagent, then the solution developed in the volumetric flasks needs to be transferred to a cuvette, the cuvette is placed on a desk type spectrophotometry instrument, multi-point calibration and measurement are carried out, the steps are complex, and the field rapid measurement is not facilitated.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the defects in the prior art, and realize the rapid determination of the zinc ion content in the field water by using a portable spectrophotometric instrument and accessories (such as a colorimetric tube, an adjustable pipettor, a buffer and a color developing agent).
The technical problem to be solved can be implemented by the following technical scheme.
A method for measuring the content of zinc ions is used for measuring the content of the zinc ions by a spectrophotometry, and is characterized by comprising the following steps:
(1) adding 7mL of standard sample (or water sample) into a 10mL colorimetric tube, adding 2mL of buffer, and uniformly mixing;
(2) then adding 1mL of color developing agent, and uniformly mixing;
(3) calibrating or measuring by spectrophotometry at the wavelength of 620nm after 10 min;
wherein the content of the first and second substances,
the buffer agent is 2g to 10g of boric acid, 1g to 6g of potassium chloride and 0.1g to 5g of sodium hydroxide in each liter of aqueous solution.
The color developing agent is 0.1-5 g of zinc reagent and 250-980 mL of absolute ethyl alcohol in each liter of aqueous solution.
In a preferred embodiment of the present invention, the buffer is prepared by:
5.10g of boric acid, 6.00g of potassium chloride and 1.38g of sodium hydroxide were weighed into a 1L measuring flask and diluted to the marked line with pure water.
As another preferred embodiment of the present invention, the developer is formulated by:
0.8g of zinc reagent is weighed, 920mL of absolute ethyl alcohol is weighed in a 1L volumetric flask, and pure water is added dropwise to the marked line.
As a further improvement of the technical scheme, the selected standard substance for calibration is a standard solution with the zinc ion content of 100mg/L of the national standard substance center.
As a further improvement of the technical scheme, the spectrophotometry measuring time period is provided by performing spectrophotometry measuring within a time period of 10 min-100 min after mixing in the step (2).
The method for measuring the zinc ion content in the technical scheme provides a simple and rapid method for measuring the zinc ion content and a method for preparing a reagent for measuring the zinc ion content; further provides a time period for measuring the zinc ion content by color development. The reagent preparation method and the determination method provided by the invention have the advantages of simple and rapid determination steps when the content of zinc ions is measured.
Detailed Description
The following provides a more detailed description of the present invention with reference to specific examples.
1. Water for experiment
The water (hereinafter referred to as water) used for preparing the reagent is laboratory first-grade water or water with the same purity.
2. Mother liquor rho of the zinc ion content standard solution is 100 mg/L:
100mg/L zinc ion standard solution produced by national center for Standard materials was purchased.
3. 0mg/L zinc ion calibration solution: laboratory first grade water.
4. 5mg/L Zinc ion calibration solution:
5.00mL of 100mg/L zinc ion standard solution produced by national center for standards substance was taken in a 100mL volumetric flask and diluted to the marked line with pure water.
5. Buffering agent: each liter of the aqueous solution contains 2g to 10g of boric acid, 1g to 6g of potassium chloride and 0.1g to 5g of sodium hydroxide.
The preferred embodiment is: 5.10g of boric acid, 6.00g of potassium chloride and 1.38g of sodium hydroxide were weighed into a 1L measuring flask and diluted to the marked line with pure water.
6. Color developing agent: each liter of the water solution contains 0.1g to 5g of zinc reagent and 250mL to 980mL of absolute ethyl alcohol.
The preferred embodiment is: 0.8g of zinc reagent is weighed, 920mL of absolute ethyl alcohol is weighed in a 1L volumetric flask, and pure water is added dropwise to the marked line.
7. Calibration of 0mg/L zinc ion calibration solution:
adding 7mL of 0mg/L zinc ion calibration solution into the colorimetric tube, then adding 2mL of buffer, and uniformly mixing; then, 1mL of a color developing agent was added, and the mixture was mixed well and subjected to the first calibration after 10 minutes.
8. Calibration of 5mg/L zinc ion calibration solution:
adding 7mL of 5mg/L zinc ion calibration solution into the colorimetric tube, adding 2mL of buffer, and uniformly mixing; and adding 1mL of color developing agent, uniformly mixing, carrying out second point calibration after 10 minutes, then ending calibration, and carrying out measurement.
9. Measuring the zinc ion content of the water sample in the range of (0 mg/L-5 mg/L):
adding 7mL of water sample into the colorimetric tube, adding 2mL of buffer, and uniformly mixing; and adding 1mL of color developing agent, uniformly mixing, and measuring after 10 minutes to obtain the zinc ion content of the water sample.
Claims (5)
1. A method for measuring the content of zinc ions is used for measuring the content of the zinc ions by a spectrophotometry, and is characterized by comprising the following steps:
(1) adding 7mL of standard sample or water sample into a 10mL colorimetric tube, adding 2mL of buffer, and uniformly mixing;
(2) then adding 1mL of color developing agent, and uniformly mixing;
(3) calibrating or measuring by spectrophotometry at the wavelength of 620nm after 10 min;
wherein the content of the first and second substances,
the buffer is a solution containing 2g to 10g of boric acid, 1g to 6g of potassium chloride and 0.1g to 5g of sodium hydroxide per liter of aqueous solution;
the color developing agent is a solution containing 0.1-5 g of zinc reagent and 250-980 mL of absolute ethyl alcohol in each liter of aqueous solution.
2. The method for determining the content of zinc ions according to claim 1, wherein the buffer is prepared by a method comprising:
5.10g of boric acid, 6.00g of potassium chloride and 1.38g of sodium hydroxide were weighed into a 1L measuring flask and diluted to the marked line with pure water.
3. The method for measuring the content of zinc ions according to claim 1, wherein the color developer is prepared by the following method:
0.8g of zinc reagent is weighed, 920mL of absolute ethyl alcohol is weighed in a 1L volumetric flask, and pure water is added dropwise to the marked line.
4. The method according to claim 1, wherein the standard substance is 100mg/L standard solution of zinc ion content of national center for standards substance.
5. The method for measuring the content of zinc ions according to claim 1, wherein the spectrophotometric measurement time period is provided within a time period of 10min to 100min after the mixing in the step (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011314693.3A CN112557317A (en) | 2020-11-20 | 2020-11-20 | Method for measuring zinc ion content |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011314693.3A CN112557317A (en) | 2020-11-20 | 2020-11-20 | Method for measuring zinc ion content |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112557317A true CN112557317A (en) | 2021-03-26 |
Family
ID=75044463
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011314693.3A Pending CN112557317A (en) | 2020-11-20 | 2020-11-20 | Method for measuring zinc ion content |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112557317A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103712980A (en) * | 2013-12-05 | 2014-04-09 | 同济大学 | Preparation and application methods for detection agent for detecting zinc content in water |
| CN103823019A (en) * | 2014-02-28 | 2014-05-28 | 江苏天瑞仪器股份有限公司 | Reagent bag used for detecting zinc ion in water |
| CN103837485A (en) * | 2014-02-28 | 2014-06-04 | 江苏天瑞仪器股份有限公司 | Method for detecting zinc ions in water mass |
| CN104458616A (en) * | 2014-12-04 | 2015-03-25 | 重庆建峰化工股份有限公司 | Determining and analyzing method of content of chelated zinc in chelated potassium and zinc urea product |
| CN206788030U (en) * | 2017-05-25 | 2017-12-22 | 苏州科特环保股份有限公司 | Zinc ion on-Line Monitor Device |
-
2020
- 2020-11-20 CN CN202011314693.3A patent/CN112557317A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103712980A (en) * | 2013-12-05 | 2014-04-09 | 同济大学 | Preparation and application methods for detection agent for detecting zinc content in water |
| CN103823019A (en) * | 2014-02-28 | 2014-05-28 | 江苏天瑞仪器股份有限公司 | Reagent bag used for detecting zinc ion in water |
| CN103837485A (en) * | 2014-02-28 | 2014-06-04 | 江苏天瑞仪器股份有限公司 | Method for detecting zinc ions in water mass |
| CN104458616A (en) * | 2014-12-04 | 2015-03-25 | 重庆建峰化工股份有限公司 | Determining and analyzing method of content of chelated zinc in chelated potassium and zinc urea product |
| CN206788030U (en) * | 2017-05-25 | 2017-12-22 | 苏州科特环保股份有限公司 | Zinc ion on-Line Monitor Device |
Non-Patent Citations (1)
| Title |
|---|
| 罗晓艳等: "循环水中锌离子测定方法的探讨", 《化肥工业》 * |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20210326 |
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