CN112553044A - Anti-blocking device for bioreactor cell culture sample inlet - Google Patents
Anti-blocking device for bioreactor cell culture sample inlet Download PDFInfo
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- CN112553044A CN112553044A CN202011435306.1A CN202011435306A CN112553044A CN 112553044 A CN112553044 A CN 112553044A CN 202011435306 A CN202011435306 A CN 202011435306A CN 112553044 A CN112553044 A CN 112553044A
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- fixedly connected
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- sample inlet
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- clogging device
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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Abstract
The invention relates to the technical field of cell culture, and discloses an anti-blocking device for a cell culture sample inlet of a bioreactor. According to the invention, the force application cam is enabled to apply force to the force-bearing circular truncated cone discontinuously by starting the micro motor, the force-bearing circular truncated cone is subjected to discontinuous force, so that the feed hopper is effectively pulled to reciprocate left and right under the action of the return spring, and then an arch structure is prevented from being formed near the sample inlet in the process of feeding material substances or cells through the left and right reciprocating motion of the feed hopper, thereby achieving the effect of effectively improving the inlet efficiency and quality of the sample inlet.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to an anti-blocking device for a cell culture sample inlet of a bioreactor.
Background
Cell culture refers to a method for simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions and the like) in vitro to enable normal cells to survive, grow, metabolize and express. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. The cell culture can be used for various disciplines of cytology, genetics, immunology, experimental medicine, oncology and the like, is a research object of molecular biology and genetic engineering, and is a material source for monoclonal antibody production, genetic engineering and the like of a biological product. However, in the in vitro cell culture process in a bioreactor, especially in a bioreactor with a culture volume of less than or equal to 10L, the chemical reaction between the supplementary material and cell metabolism and other substances in the culture system causes the supplementary material to be separated out and crystallized at the sample inlet and block the sample inlet, which causes culture termination and experiment failure, greatly wastes resources such as manpower and material resources, and the like, and the supplementary material or the cells form an arch structure near the sample inlet in the sample inlet process, thereby greatly affecting the normal sample injection efficiency and quality.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides an anti-blocking device for a cell culture sample inlet of a bioreactor.
In order to achieve the purpose, the invention adopts the following technical scheme:
an anti-blocking device for a cell culture sample inlet of a bioreactor comprises a sample inlet pipe, wherein a protective rubber ring is arranged on the outer surface of the sample inlet pipe, the inner wall of the sample inlet pipe is fixedly connected with a fixing ring, a mounting ring is arranged above the fixing ring, the two sides of the mounting ring are in threaded connection with fixing screws, the bottom ends of the fixing screws are in threaded connection with the upper surface of the fixing ring, the top end of the fixed screw is fixedly connected with a force application knob, the inner surfaces of the left side and the right side of the mounting ring are both fixedly connected with two return springs, one end of each of the two return springs close to each other is fixedly connected with the same feed hopper, the inner surface of the left side of the mounting ring is fixedly connected with a micro motor positioned between two return springs, the output fixedly connected with application of force cam on micro motor right side, the left side fixedly connected with of feeder hopper is located the atress round platform at the application of force cam back.
Preferably, fixedly connected with is located the direction slide rail between two reset spring on the internal surface on installing ring right side, the right side of feeder hopper is passed through guide bar sliding connection and is on the internal surface of direction slide rail.
Preferably, the curved surface of the stressed round table is fixedly connected with a sliding-assistant pad, and the outer surface of the force application cam is fixedly connected with a wear-resistant pad.
Preferably, the return spring has a spring constant of thirty newtons per centimeter.
Preferably, the length of the fixing screw is not less than three centimeters, and the length of the thread on the outer surface of the fixing screw is not less than five-half centimeters.
Preferably, the connection mode between the sampling tube and the protective rubber ring is interference connection.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, the force application cam is enabled to apply force to the force-bearing circular truncated cone discontinuously by starting the micro motor, the force-bearing circular truncated cone is subjected to discontinuous force, so that the feed hopper is effectively pulled to reciprocate left and right under the action of the return spring, and then an arch structure is prevented from being formed near the sample inlet in the process of feeding material substances or cells through the left and right reciprocating motion of the feed hopper, thereby achieving the effect of effectively improving the inlet efficiency and quality of the sample inlet.
Drawings
FIG. 1 is a schematic view of the internal structure of the present invention;
FIG. 2 is an enlarged view taken at A in FIG. 1;
fig. 3 is an enlarged view of fig. 1 at B.
In the figure: 1. a sample inlet pipe; 2. a protective rubber ring; 3. a fixing ring; 4. a mounting ring; 5. fixing the screw rod; 6. a force application knob; 7. a return spring; 8. a feed hopper; 9. a micro motor; 10. a force application cam; 11. a stressed round table; 12. a guide slide rail; 13. a guide rod.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Referring to fig. 1-3, an anti-clogging device for a cell culture sample inlet of a bioreactor comprises a sample inlet pipe 1, a protective rubber ring 2 is arranged on the outer surface of the sample inlet pipe 1, the sample inlet pipe 1 and the protective rubber ring 2 are connected in an interference manner, a fixing ring 3 is fixedly connected to the inner wall of the sample inlet pipe 1, a mounting ring 4 is arranged above the fixing ring 3, fixing screws 5 are connected to the two sides of the mounting ring 4 in a threaded manner, the length of each fixing screw 5 is not less than three centimeters, the length of the thread on the outer surface of each fixing screw 5 is not less than five centimeters, the bottom end of each fixing screw 5 is connected to the upper surface of the corresponding fixing ring 3 in a threaded manner, a force application knob 6 is fixedly connected to the top end of each fixing screw 5, two reset springs 7 are fixedly connected to the inner surfaces of the left side and the right side, the elastic coefficient of reset spring 7 is thirty newton per centimeter, both sides the same feeder hopper 8 of one end fixedly connected with that reset spring 7 is close to each other, fixedly connected with is located the micro motor 9 between two reset spring 7 on the left internal surface of collar 4, the output end fixedly connected with application of force cam 10 on micro motor 9 right side, the left side fixedly connected with of feeder hopper 8 is located the atress round platform 11 at the application of force cam 10 back, fixedly connected with helps smooth the pad on the curved surface of atress round platform 11, fixedly connected with wear pad on the surface of application of force cam 10, fixedly connected with is located the direction slide rail 12 between two reset spring 7 on the internal surface on collar 4 right side, 13 sliding connection of guide bar is passed through on the right side of feeder hopper 8 on the internal surface of direction slide rail 12.
In the invention, when a user uses the device, the force application cam 10 is enabled to apply force to the force bearing circular truncated cone 11 discontinuously by starting the micro motor 9, the force bearing circular truncated cone 11 is intermittently stressed to effectively pull the feed hopper 8 to reciprocate left and right under the action of the return spring 7, and then the feed hopper 8 reciprocates left and right to prevent feed supplement substances or cells from forming an arch structure near a sample inlet in the entering process, so that the effect of effectively improving the entering efficiency and quality of the sample inlet is achieved.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Claims (6)
1. The utility model provides a bioreactor cell culture anti-clogging device for inlet, includes into appearance pipe (1), its characterized in that, be provided with protection rubber ring (2) on the surface of appearance pipe (1), fixedly connected with solid fixed ring (3) on the inner wall of appearance pipe (1), the top of solid fixed ring (3) is provided with collar (4), the both sides threaded connection of collar (4) has clamping screw (5), the bottom threaded connection of clamping screw (5) is at the upper surface of solid fixed ring (3), the top fixedly connected with application of force knob (6) of clamping screw (5), equal two reset spring (7) of fixedly connected with on the internal surface of collar (4) left and right sides, both sides the same feeder hopper (8) of one end fixedly connected with that reset spring (7) are close to each other, fixedly connected with is located the micro motor between two reset spring (7) on the left internal surface of collar (4) (9) The output fixedly connected with application of force cam (10) on micro motor (9) right side, the left side fixedly connected with of feeder hopper (8) is located atress round platform (11) at application of force cam (10) back.
2. The anti-clogging device for the cell culture sample inlet of the bioreactor according to claim 1, wherein a guide rail (12) between the two return springs (7) is fixedly connected to the inner surface of the right side of the mounting ring (4), and the right side of the feed hopper (8) is slidably connected to the inner surface of the guide rail (12) through a guide rod (13).
3. The anti-clogging device for the cell culture sample inlet of the bioreactor according to claim 1, wherein a sliding pad is fixedly connected to the curved surface of the force-bearing circular platform (11), and a wear pad is fixedly connected to the outer surface of the force-applying cam (10).
4. The anti-clogging device for the cell culture inlet of the bioreactor according to claim 1, wherein the elastic coefficient of the return spring (7) is thirty newtons per centimeter.
5. The anti-clogging device for the cell culture sample inlet of the bioreactor according to claim 1, wherein the length of the fixing screw (5) is not less than three centimeters, and the length of the thread on the outer surface of the fixing screw (5) is not less than five-half centimeters.
6. The anti-clogging device for the cell culture sample inlet of the bioreactor according to claim 1, wherein the connection mode between the sample inlet tube (1) and the protective rubber ring (2) is interference connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011435306.1A CN112553044A (en) | 2020-12-10 | 2020-12-10 | Anti-blocking device for bioreactor cell culture sample inlet |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011435306.1A CN112553044A (en) | 2020-12-10 | 2020-12-10 | Anti-blocking device for bioreactor cell culture sample inlet |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112553044A true CN112553044A (en) | 2021-03-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011435306.1A Withdrawn CN112553044A (en) | 2020-12-10 | 2020-12-10 | Anti-blocking device for bioreactor cell culture sample inlet |
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| CN (1) | CN112553044A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1175821A (en) * | 1997-09-01 | 1999-03-23 | Res Dev Corp Of Japan | Biochemical reaction apparatus for culturing several series of cell and biochemical reaction |
| JP2017029162A (en) * | 2011-09-16 | 2017-02-09 | アムジエン・インコーポレーテツド | Pre-programmed non-feedback controlled continuous feeding of cell cultures |
| CN207435421U (en) * | 2017-10-24 | 2018-06-01 | 江西诚志生物工程有限公司 | A kind of D-ribose production antifouling type installation for fermenting |
| CN207827206U (en) * | 2018-01-25 | 2018-09-07 | 韶关市龙凤胎饲料有限公司 | A kind of variable frequency vibrating-type feeding mechanism of feeding stuff cuber |
| CN210251465U (en) * | 2020-01-09 | 2020-04-07 | 广州加泰医药科技有限公司 | A novel filter equipment for medicine research and development |
| CN210496783U (en) * | 2019-08-12 | 2020-05-12 | 合肥中都机械有限公司 | Feeding pretreatment device for roller press |
| CN210791658U (en) * | 2019-07-02 | 2020-06-19 | 荆州市荆洪塑料制品有限公司 | Plastic products raw materials for production is collected and is used extrusion device |
| CN211544619U (en) * | 2020-01-16 | 2020-09-22 | 江西省欧陶科技有限公司 | Arch breaking device of feeding machine |
-
2020
- 2020-12-10 CN CN202011435306.1A patent/CN112553044A/en not_active Withdrawn
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1175821A (en) * | 1997-09-01 | 1999-03-23 | Res Dev Corp Of Japan | Biochemical reaction apparatus for culturing several series of cell and biochemical reaction |
| JP2017029162A (en) * | 2011-09-16 | 2017-02-09 | アムジエン・インコーポレーテツド | Pre-programmed non-feedback controlled continuous feeding of cell cultures |
| CN207435421U (en) * | 2017-10-24 | 2018-06-01 | 江西诚志生物工程有限公司 | A kind of D-ribose production antifouling type installation for fermenting |
| CN207827206U (en) * | 2018-01-25 | 2018-09-07 | 韶关市龙凤胎饲料有限公司 | A kind of variable frequency vibrating-type feeding mechanism of feeding stuff cuber |
| CN210791658U (en) * | 2019-07-02 | 2020-06-19 | 荆州市荆洪塑料制品有限公司 | Plastic products raw materials for production is collected and is used extrusion device |
| CN210496783U (en) * | 2019-08-12 | 2020-05-12 | 合肥中都机械有限公司 | Feeding pretreatment device for roller press |
| CN210251465U (en) * | 2020-01-09 | 2020-04-07 | 广州加泰医药科技有限公司 | A novel filter equipment for medicine research and development |
| CN211544619U (en) * | 2020-01-16 | 2020-09-22 | 江西省欧陶科技有限公司 | Arch breaking device of feeding machine |
Non-Patent Citations (1)
| Title |
|---|
| 杨晓伟 等: "进料量及频率对振动给料机物料消耗功率的影响", 《机械工程师》 * |
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Application publication date: 20210326 |