CN112551692A

CN112551692A - Halomonas with aerobic denitrification and heterotrophic sulfur oxidation functions and application thereof

Info

Publication number: CN112551692A
Application number: CN202011324290.7A
Authority: CN
Inventors: 邵宗泽; 王丽萍; 李建洋
Original assignee: China Ocean Mineral Resources R & D Association (china's Ocean Affairs Administration); Third Institute of Oceanography MNR
Current assignee: China Ocean Mineral Resources R & D Association (china's Ocean Affairs Administration); Third Institute of Oceanography MNR
Priority date: 2020-11-23
Filing date: 2020-11-23
Publication date: 2021-03-26
Anticipated expiration: 2040-11-23
Also published as: CN112551692B

Abstract

Halomonas with aerobic denitrification and heterotrophic sulfur oxidation functions and application thereof, belonging to the technical field of microorganism application. Two Halomonas (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718 with aerobic denitrification and heterotrophic sulfur oxidation functions are provided, and the preservation numbers are as follows: GDMCC No. 60985 and GDMCC No. 60984. The Halomonas (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718 can be applied to the denitrification and sulfur removal, can be suitable for the biological denitrification treatment of various nitrogen-containing wastewater, have high-efficiency denitrification and heterotrophic sulfur oxidation capability under aerobic and anoxic conditions, can quickly and efficiently remove nitrate, nitrite, ammonia nitrogen, sulfide and the like, and have better application prospect in the biological denitrification and sulfur removal of sewage, sludge, sediment or aquaculture water.

Description

Halomonas with aerobic denitrification and heterotrophic sulfur oxidation functions and application thereof

Technical Field

The invention belongs to the technical field of microorganism application, and particularly relates to halomonas with aerobic denitrification and heterotrophic sulfur oxidation functions and application thereof.

Background

The large discharge of industrial waste water and the long-term large application of fertilizers in modern agriculture result in the pollution of underground water and surface water by nitrogen-containing compounds such as nitrate, nitrite and ammonia nitrogen, which directly affects the quality of drinking water of people. With the intensive high-density culture of the aquaculture industry, excessive baits are thrown into culture water, so that the culture environment is rapidly deteriorated, and a large amount of ammonia nitrogen, nitrate, nitrite and the like are accumulated to cause frequent diseases of cultured animals. Hydrogen sulfide is also a toxic waste gas widely present in sewage treatment plants, livestock farming, composting plants, and the like.

Compared to physical and chemical methods, biological methods for removing nitrates and sulfides are considered economically feasible and environmentally friendly. In the biological method, sulfur-oxidizing denitrifying bacteria play a crucial role in removing nitrate and sulfide. Microorganisms with denitrification and sulfur oxidation functions are widely distributed in natural environment, and can utilize nitrate or nitrite and the like as electron acceptors, reduce the nitrate into nitrogen through denitrification, and simultaneously oxidize sulfide, elemental sulfur, thiosulfate and the like as electron donors to generate elemental sulfur or sulfate. These microorganisms include two major types, autotrophic sulfur-oxidizing denitrifying bacteria, and heterotrophic or facultative sulfur-oxidizing denitrifying bacteria. The growth of the former is easily inhibited by organic matters, and is difficult to form larger biomass compared with heterotrophic bacteria which grow slowly; the latter can simultaneously utilize organic matters and reducing sulfur-containing compounds as electron donors to carry out denitrification and can synchronously remove the organic matters, sulfides and nitrates. Therefore, the denitrifying bacteria with heterotrophic sulfur oxidation are separated and identified, and are applied to the removal of carbon, nitrogen and sulfur in industrial wastewater and aquaculture water, so that the method has an important application prospect.

Chinese patent CN201610562243.3 discloses a facultative autotrophic sulfur-oxidizing denitrifying rhizobium F43b and application thereof. Rhizobia (Rhizobium sp.) F43bT was deposited at the Chinese Collection for Type Culture Collection (CCTCC) at 2016, 3, 30 days, address: the preservation number of Wuhan university in Wuhan City of China is CCTCC NO: m2016158. Rhizobium (Rhizobium sp.) F43bT is a facultative autotrophic Rhizobium with a sulfur oxidation denitrification function, can simultaneously remove COD, nitrate and sulfide in polluted water or sediments, realizes the removal of carbon, nitrogen and sulfur in the polluted water or sediment treatment, and provides strains and microbial preparations for river restoration and carbon, nitrogen and sulfur wastewater treatment; meanwhile, the research of the rhizobium provides theoretical guidance for the application of the rhizobium in riverway restoration and carbon, nitrogen and sulfur removal.

Disclosure of Invention

The invention aims to provide the halomonas with the functions of aerobic denitrification and heterotrophic sulfur oxidation, which can efficiently perform denitrification and sulfur oxidation under aerobic and anoxic conditions, can rapidly and efficiently remove nitrate, nitrite, ammonia nitrogen and sulfide, and performs denitrification and sulfur removal on sewage, sludge, sediments or aquaculture water.

The invention also aims to provide the application of the halomonas with the functions of aerobic denitrification and heterotrophic sulfur oxidation in the denitrification and sulfur removal.

The Halomonas with the aerobic denitrification and heterotrophic sulfur oxidation functions is Halomonas (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718, which are preserved in Guangdong province microorganism strain preservation center in 03-27.2020, and the preservation address is No. 59 building 5 of the large institute of Xielianlu 100 in Guangzhou city; the preservation numbers are respectively: GDMCC No. 60985 and GDMCC No. 60984.

The Halomonas (Halomonasp.) MCCC 1A17488 is obtained by directional enrichment and separation from sulfide in hydrothermal area of Tianhui in southwest Indian province, and is a new species of Halomonas, and is named as Halomonas (Halomonas sp.) BC-M4-4.

The Halomonas (Halomonas sp.) MCCC 1A13718 is obtained by directional enrichment and separation from deep sea sediments in the Pacific ocean, is a new species of Halomonas, and is named as Halomonas (Halomonas sp.) NLG _ F1E.

The aerobic denitrification of the halomonas (Halomonasp.) MCCC 1A17488 and MCCC 1A13718 is characterized in that: the genome of the strain contains membrane-bound nitrateThe nucleotide-binding nitrate reductase (NAR), periplasmic nitrate reductase (NAP), nitrite reductase (NIR), Nitric Oxide Reductase (NOR), and nitrous oxide reductase (NOS) genes. In nitrogen-containing simulated wastewater, the Halomonas (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718 can simulate 15mM NO in wastewater within 24h₃ ^-N is completely removed, no nitrite is accumulated, the high-efficiency denitrification capability is realized, and the gas product of the aerobic denitrification is N₂And a small amount of N₂O。

Heterotrophic sulfur oxidation of halomonas (halomonas sp.) MCCC 1a17488 and MCCC 1a13718 is characterized by: the genome contains the genes of sulfide quinone oxidoreductase (Sqr), flavin cytochrome c sulfide dehydrogenase (FCSD) and thiosulfate dehydrogenase (TsdA). The Halomonas (Halomonas sp) MCCC 1A17488 and MCCC 1A13718 were able to deliver 1mM exogenous Na within 1h in a 50mM HEPES buffer system₂S is completely oxidized, and the main product comprises sodium thiosulfate. Meanwhile, the Halomonas (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718 can convert Na₂S₂O₃Oxidized to tetrathionate.

The Halomonas (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718 with the functions of aerobic denitrification and heterotrophic sulfur oxidation can be applied to denitrification and sulfur removal.

The Halomonas (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718 with the functions of aerobic denitrification and heterotrophic sulfur oxidation can be applied to the removal of nitrate, nitrite, ammonia nitrogen or sulfide in sewage, sludge, sediment or aquaculture water.

Compared with the prior art, the invention has the following outstanding effects:

1. the Halomonas (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718 of the invention can carry out rapid growth and high-efficiency denitrification by taking nitrate, nitrite or ammonium salt as a unique nitrogen source, and can be suitable for biological denitrification treatment of various nitrogen-containing wastewater. Denitrification can be carried out under the aerobic and anaerobic conditions, and the final gas products are nitrogen and a small amount of nitrous oxide, so that the method is environment-friendly; the addition of ammonia nitrogen can obviously improve the reduction rate of nitrate and nitrite.

2. The Halomonas (Halomonas sp.) mcc 1a17488 and mcc 1a13718 of the present invention have a very efficient ability to oxidize exogenous hydrogen sulfide. Compared with autotrophic microorganisms, the microorganisms have the characteristics of rapid growth, large biomass and economic and convenient culture. The Halomonas (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718 of the invention also have high hydrogen sulfide tolerance and can oxidize and tolerate 7mM Na and 3mM Na respectively₂S。

3. The Halomonas (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718 of the invention are from marine habitat, the salinity adaptability range is wide, and the characteristic greatly increases the application of the Halomonas (Halomonas sp.) MCCC 1A17488 and the MCCC 1A13718 in the high-salinity wastewater treatment.

Drawings

FIG. 1 shows the effect of Halomonas sp MCCC 1A17488(BC-M4-4) and MCCC 1A13718(NLG _ F1E) and 5 reference strains on the removal of nitrate nitrogen from simulated wastewater under optimum conditions (right ordinate corresponds to NO)₃ ^-Removal rate of-N and cell concentration OD₆₀₀Two values).

FIG. 2 is a graph of sodium sulfide oxidation capacity of Halomonas sp MCCC 1A17488(BC-M4-4) and MCCC 1A13718(NLG _ F1E).

FIG. 3 is a graph of the ability of Halomonas sp MCCC 1A17488(BC-M4-4) and MCCC 1A13718(NLG _ F1E) to oxidize sodium sulfide to produce thiosulfate.

FIG. 4 is a graph of the thiosulfate oxidation potential of Halomonas sp MCCC 1A17488(BC-M4-4) and MCCC 1A13718(NLG _ F1E).

FIG. 5 is a graph of the ability of Halomonas (Halomonassp.) MCCC 1A17488 and MCCC 1A13718 to oxidize thiosulfate to produce tetrathionate.

Detailed Description

The following examples will further illustrate the present invention with reference to the accompanying drawings, but the present invention is not limited thereto. The methods in the following examples are conventional methods unless otherwise specified.

Example 1 isolation and characterization of Halomonas salina (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718

1 isolation of the strains: taking sulfide samples in hydrothermal areas of Tianhu in southwest Indian ocean, adopting a gradient dilution coating plate method, and coating a plate by using a Marine Agar solid culture medium for separation and purification to obtain monocellular Halomonas (Halomonas sp.) MCCC 1A17488 monocultures. The halomonas has been deposited in Guangdong provincial microorganism strain collection center at 03-27.2020, with the respective preservation numbers: GDMCC No. 60985. Taking a sample of the Pacific deep sea sediment, adopting a gradient dilution plating method, and using a Marine Agar solid culture medium for plating, separating and purifying to obtain the monocell MCCC 1A13718 monocell. The halomonas has been deposited in Guangdong provincial microorganism strain collection center at 03-27.2020, with the respective preservation numbers: GDMCC No. 60984.

Physiological and biochemical characteristics of 2 strains

Physiological and biochemical characteristics of Halomonas (Halomonas sp.) MCCC 1a 17488:

morphological characteristics: halomonas sp MCCC 1A17488, gram negative, cultured on Marine Agar solid culture medium at 32 ℃ for 24h, the colony is beige, smooth in surface, slightly convex, regular in edge and opaque, and the diameter of the colony is about 0.6-1 mm.

Physiological and biochemical characteristics: the growth salinity of the strain is 0-18%, more than 18% of the strain does not grow, and the most suitable is 2-6%; the growth pH is 6-10, most preferably 7-8. The growth temperature is 4-50 ℃, and the optimal growth temperature is 37-40 ℃. Oxidase (+), catalase (+), nitrate reduction (+), indole test (-), and can ferment D-glucose to produce acid, gelatin liquefaction (+), and urease (-), and can utilize D-glucose, L-arabinose, D-mannitol, maltose, adipic acid, malic acid, sodium citrate and the like. Has enzyme activities of alkaline phosphatase, esterase, lipoid esterase, lipase, arylamine leucine, aminopeptidase valine, arylamine cystine, trypsin, acid phosphatase, alpha-glucosidase, etc.

Physiological and biochemical characteristics of Halomonas sp MCCC 1A 13718:

morphological characteristics: halomonas sp MCCC 1A13718 which is gram negative is cultured on a Marine Agar solid culture medium for 24 hours at 32 ℃, the colony is beige, the surface is smooth, the edge is regular and opaque, and the diameter of the colony is about 0.5-0.8 mm.

Physiological and biochemical characteristics: the growth salinity of the strain is 0-20%, most preferably 2-8%; the growth pH is 6-10, most preferably 7-8. The growth temperature is 4-55 ℃, and the optimal growth temperature is 37-40 ℃. Oxidase (+), catalase (+), nitrate reduction (+), indole test (-), and D-glucose can be fermented to produce acid, gelatin liquefaction (-), urease (-), and D-glucose, L-arabinose, D-mannitol, maltose, adipic acid, malic acid, sodium citrate and the like can be utilized. Has alkaline phosphatase, esterase, lipoid esterase, lipase, leucine arylamine enzyme, valine amino peptide

Molecular characterization of the strains

Genomic DNA of Halomonas salina (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718 was extracted according to a conventional method, and 16S rRNA gene sequence and genomic sequence were determined. The 16S rRNA gene of Halomonas salina (Halomonas sp.) MCCC 1A17488 was compared with Halomonas lactosuvorans KCTC 52281 by BLAST^TThe similarity is highest and is 99.4%; 16S rRNA gene of Halomonas (Halomonas sp.) MCCC 1A13718 and Halomonas saliphila LCB169^TThe highest similarity was 98.8%. Halomonas (Halomonas sp.) MCCC 1a17488 and MCCC 1a13718 had an average nucleotide similarity ANI value of less than 95% with the most recent model species and a digital DNA-DNA correlation of less than 70% with the most recent model species.

By integrating the morphological characteristics, physiological and biochemical characteristics, 16S rRNA gene sequence analysis and genome analysis results of the strain, the Halomonas (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718 belong to the genus Halomonas, are two potential new species of the genus, and specifically are the Halomonas (Halomonas sp.) MCCC 1A17488 and MCCC 1A 13718. The 16S rRNA gene sequences of the halomonas MCCC 1A17488 and MCCC 1A13718 are shown as SEQ ID NO.1 and SEQ ID NO. 2.

Example 2 Halomonas salina (Halomonasp.) MCCC 1A17488 and MCCC 1A13718 were optimized for denitrification conditions in simulated wastewater

Scraping fresh thallus from MA plate, re-suspending with sterile seawater, and adjusting the concentration of the thallus to 10⁹cfu/mL, inoculated with an inoculum size of 0.2% to 15mM NO₃ ^--N or NO₂ ^-And (4) in the simulated wastewater of the-N, measuring the denitrification conditions of the strains under different carbon sources, pH values, temperatures, salinity and C/N ratios, and optimizing denitrification conditions. Simulating the components of wastewater: KH (Perkin Elmer)₂PO₄ 2g/L，Na₂HPO₄ 3g/L，MgSO₄·7H₂O 0.05g/L、FeSO₄·7H₂O 0.02g/L、CaCL₂0.02g/L, 1000mL of distilled water, and sterilizing at 121 ℃ for 20 min. The nitrate nitrogen is measured by a GB T12763 zinc-cadmium reduction method; the nitrite nitrogen determination adopts GB7493-87 naphthyl ethylenediamine hydrochloride spectrophotometry.

The denitrification condition optimization result shows that denitrification electron donors of Halomonas (Halomonas sp.) MCCC 1A17488 comprise sodium acetate, sodium propionate, sodium citrate, sodium succinate, glucose, sucrose, sodium lactate, maltose, glycerol, mannitol and the like, and optimally sodium citrate and glucose; C/N is 2-18, preferably 4-18; the pH value is 7-9, and the preferable pH value is 8; the salinity is 0-80 g/L, preferably 30-40 g/L; the temperature is 15-45 ℃, and preferably 37 ℃.

The denitrification condition optimization result shows that denitrification electron donors of Halomonas (Halomonas sp.) MCCC 1A13718 comprise sodium acetate, sodium propionate, sodium citrate, sodium succinate, glucose, sodium lactate, maltose, ethanol, glycerol, mannitol and the like, and the optimal is the sodium citrate; C/N is 4-18, preferably 5-18; the pH value is 7-9, and the preferable pH value is 8; the salinity is 0-100 g/L, preferably 30-40 g/L; the temperature is 15-45 ℃, and preferably 37 ℃.

At 15mM NO₃ ^-N is a unique nitrogen source, sodium citrate is a unique carbon source, C/N is 8, pH is 7.6, 37 ℃ and is cultured under the condition of shaking at 160r/min, and both halomonas (halomonas sp.) MCCC 1A17488 and MCCC 1A13718 can completely remove nitrate within 24h without nitrite residues (figure 1).

EXAMPLE 3 measurement of sodium sulfide Oxidation Rate of Halomonas (Halomonasp.) MCCC 1A17488 and MCCC 1A13718

Culturing Halomonas (Halomonasp) MCCC 1A17488 and MCCC 1A13718 in MB liquid culture medium for 24 hr under shaking, centrifuging to collect thallus, washing with normal saline twice, and suspending in HEPES buffer solution of pH7.4 to obtain bacterial suspension with final bacterial turbidity OD ₆₀₀2. 10mL of the bacterial suspension is taken, freshly prepared sodium sulfide is added to start the reaction (the final concentration is 1mM), and samples are taken at five time points of 0h, 0.5h, 1h, 1h and 2h respectively to determine the residual concentration of the sodium sulfide in the system. And after the reaction is finished, centrifuging again to collect thalli, cleaning twice in ultrapure water, transferring the thalli to a freeze dryer for freeze drying for 24 hours, and measuring the dry weight of cells. From the change in sodium sulfide concentration and the dry cell weight, the rate of oxidation of sodium sulfide per bacterial cell dry weight per unit time was calculated. Meanwhile, the centrifuged supernatant was collected, and the product of sulfide oxidation was measured by a liquid chromatograph.

The results show that Halomonas (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718 both have high sodium sulfide oxidation capacity and can oxidize 1mM exogenous Na within 1h₂S is completely oxidized and the oxidation products mainly include sodium thiosulfate (fig. 2 and 3). The sodium sulfide oxidation rates of Halomonas sp MCCC 1A17488 and MCCC 1A13718 were 50 and 54. mu. mol min, respectively^-1g^-1(cell dry weight).

Example 4 measurement of the oxidizing Capacity of Halomonas sp MCCC 1A17488 and MCCC 1A13718 sodium thiosulfate

Culturing Halomonas (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718 in MB liquid culture medium for 24 hr under shaking, centrifuging to collect thallus, washing with normal saline twice, and suspending in HEPES buffer solution of pH7.4 to obtain bacterial suspension with final bacterial turbidity of OD ₆₀₀2. 10mL of the bacterial suspension is added with a freshly prepared sodium thiosulfate solution (the final concentration is 1.5mM), and the residual concentration of the sodium thiosulfate in the system and the concentration of the product tetrathionate are respectively determined by sampling at four time points of 0h, 1h, 2h and 3 h. The results show that Halomonas sp MCCC 1A1748Both MCCC 1a13718 and MCCC 1a13718 have high sodium thiosulfate oxidation capacity, and the product is tetrathionate (fig. 4 and 5).

Example 5 Sulfur Oxidation function of Halomonas salina (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718 under aerobic Denitrification conditions

Culturing Halomonas (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718 in MB liquid culture medium with shaking for 24 hr, centrifuging to collect thallus, washing with normal saline twice, and inoculating to denitrifying culture medium (KH)₂PO₄2g/L、Na₂HPO₄ 3g/L、MgSO₄·7H₂O 0.05g/L、FeSO₄·7H₂O 0.02g/L、CaCL₂0.02g/L, 1000mL of distilled water, 15mM NO₃ ^--N as sole nitrogen source, sodium citrate as sole carbon source, C/N8, pH 7.6), freshly prepared Na was added₂S, the medium was allowed to reach a final concentration of 1mM, 3mM, 5mM, 7mM, 9mM and 12mM, and shaking-cultured at 37 ℃ and 160r/min, and samples were taken every 24 hours to determine NO₃ ^--N、NO₂ ^--N、Na₂S concentration and OD₆₀₀. The results show that Halomonas (Halomonasp.) MCCC 1A17488 and MCCC 1A13718 can completely remove nitrate within 24h without nitrite accumulation, and they can oxidize Na within 48h₂The highest S concentrations were 7mM and 3mM, respectively.

The halomonas screened by the method can efficiently perform denitrification and sulfur oxidation under aerobic and anoxic conditions, can rapidly and efficiently remove nitrate, nitrite, ammonia nitrogen and sulfide, and has strong application value in denitrification and sulfur removal of sewage, sludge, sediments or aquaculture water.

Sequence listing

<110> third oceanographic institute of natural resources department; china ocean mineral resources research and development association (China ocean affairs administration)

<120> halomonas with aerobic denitrification and heterotrophic sulfur oxidation functions and application thereof

<160> 2

<170> SIPOSequenceListing 1.0

<210> 2

<211> 1395

<212> DNA

<213> Halomonas MCCC 1A13718(Halomonas sp. MCCC 1A13718)

<400> 2

tcgagcggca gcacggggag cttgctccct ggtggcgagc ggcggacggg tgagtaatgc 60

ataggaatct gcccggtagt gggggataac ctggggaaac ccaggctaat accgcatacg 120

tcctacggga gaaagcaggg gctcttcgga ccttgcgcta tcggatgagc ctatgtcgga 180

ttagctggtt ggtgaggtaa tggctcacca aggcgacgat ccgtagctgg tctgagagga 240

tgatcagcca catcgggact gagacacggc ccgaactcct acgggaggca gcagtgggga 300

atattggaca atgggggcaa ccctgatcca gccatgccgc gtgtgtgaag aaggccctcg 360

ggttgtaaag cactttcagt ggggaagaac gcctgacggt taatacccgt cagggaagac 420

atcacccaca gaagaagcac cggctaactc cgtgccagca gccgcggtaa tacggagggt 480

gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggcttga taagccggtt 540

gtgaaagccc cgggctcaac ctgggaacgg catccggaac tgtcaggcta gagtgcagga 600

gaggaaggta gaattcccgg tgtagcggtg aaatgcgtag agatcgggag gaataccagt 660

ggcgaaggcg gccttctgga ctgacactga cgctgaggtg cgaaagcgtg ggtagcaaac 720

aggattagat accctggtag tccacgccgt aaacgatgtc gactagccgt tgggtccttc 780

gcggactttg tggcgcagtt aacgcgataa gtcgaccgcc tggggagtac ggccgcaagg 840

ttaaaactca aatgaattga cgggggcccg cacaagcggt ggagcatgtg gtttaattcg 900

atgcaacgcg aagaacctta cctacccttg acatcctgcg aacccttcgg agacgaaggg 960

gtgccttcgg gaacgcagag acaggtgctg catggctgtc gtcagctcgt gttgtgaaat 1020

gttgggttaa gtcccgtaac gagcgcaacc cttgtcccta tttgccagcg attcggtcgg 1080

gaactctagg gagactgccg gtgacaaacc ggaggaaggt ggggacgacg tcaagtcatc 1140

atggccctta cgggtagggc tacacacgtg ctacaatggt cggtacaaag ggttgcgagc 1200

tcgcgagggc aagccaatcc cagaaagccg atctcagtcc ggatcggagt ctgcaactcg 1260

actccgtgaa gtcggaatcg ctagtaatcg tgaatcagaa tgtcacggtg aatacgttcc 1320

cgggccttgt acacaccgcc cgtcacacca tgggagtgga ctgcaccaga agtggttagc 1380

ctaaccttcg ggagg 1395

<210> 2

<211> 1368

<212> DNA

<213> Halomonas MCCC 17488(Halomonas sp. MCCC 1A17488)

<400> 2

agcttgctcc ctggtggcga gcggcggacg ggtgagtaat gcataggaat ctgcccggta 60

gtgggggata acctggggaa acccaggcta ataccgcata cgtcctacgg gagaaagcag 120

gggaccttcg ggccttgcgc tatcggatga gcctatgtcg gattagctgg ttggtgaggt 180

aacggctcac caaggcgacg atccgtagct ggtctgagag gatgatcagc cacatcggga 240

ctgagacacg gcccgaactc ctacgggagg cagcagtggg gaatattgga caatgggcgc 300

aagcctgatc cagccatgcc gcgtgtgtga agaaggccct cgggttgtaa agcactttca 360

gtggggaaga acgccttccg gctaataccc ggaaggaaag acatcaccca cagaagaagc 420

accggctaac tccgtgccag cagccgcggt aatacggagg gtgcgagcgt taatcggaat 480

tactgggcgt aaagcgcgcg taggcggctt gataagccgg ttgtgaaagc cccgggctca 540

acctgggaac ggcatccgga actgtcaggc tagagtgcag gagaggaagg tagaattccc 600

ggtgtagcgg tgaaatgcgt agagatcggg aggaatacca gtggcgaagg cggccttctg 660

gactgacact gacgctgagg tgcgaaagcg tgggtagcaa acaggattag ataccctggt 720

agtccacgcc gtaaacgatg tcgactagcc gttgggtcct tcgcggactt tgtggcgcag 780

ttaacgcgat aagtcgaccg cctggggagt acggccgcaa ggttaaaact caaatgaatt 840

gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgatgcaacg cgaagaacct 900

tacctaccct tgacatcctg cgaacccttc ggagacgaag gggtgccttc gggaacgcag 960

agacaggtgc tgcatggctg tcgtcagctc gtgttgtgaa atgttgggtt aagtcccgta 1020

acgagcgcaa cccttgtccc tatttgccag cgattcggtc gggaactcta gggagactgc 1080

cggtgacaaa ccggaggaag gtggggacga cgtcaagtca tcatggccct tacgggtagg 1140

gctacacacg tgctacaatg gtcagtacaa agggttgcga acttgcgaga gtgagccaat 1200

cccagaaagc tgatctcagt ccggatcgga gtctgcaact cgactccgtg aagtcggaat 1260

cgctagtaat cgtgaatcag aatgtcacgg tgaatacgtt cccgggcctt gtacacaccg 1320

cccgtcacac catgggagtg gactgcacca gaagtaggtt agcctaac 1368

Claims

1. The Halomonas with the functions of aerobic denitrification and heterotrophic sulfur oxidation is characterized in that the Halomonas is Halomonas (Halomonas sp.) MCCC 1A17488 and MCCC 1A13718, which is preserved in Guangdong provincial microorganism strain preservation center at 03-27.2020, and the preservation numbers are respectively: GDMCC No. 60985 and GDMCC No. 60984.

2. The use of the halomonas having aerobic denitrification and heterotrophic sulfur oxidation functions as claimed in claim 1 in denitrification and sulfur removal.

3. The use of the halomonas having aerobic denitrification and heterotrophic sulfur oxidation functions as claimed in claim 1 for removing nitrate, nitrite, ammonia nitrogen or sulfides from sewage, sludge, sediment or aquaculture water.