CN112544869A - Cereal thick slurry matrix and preparation method thereof - Google Patents
Cereal thick slurry matrix and preparation method thereof Download PDFInfo
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- CN112544869A CN112544869A CN202011393788.9A CN202011393788A CN112544869A CN 112544869 A CN112544869 A CN 112544869A CN 202011393788 A CN202011393788 A CN 202011393788A CN 112544869 A CN112544869 A CN 112544869A
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- grinding
- slurry
- cereal
- soaking
- pulp
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- 235000013339 cereals Nutrition 0.000 title claims abstract description 68
- 239000002002 slurry Substances 0.000 title claims abstract description 43
- 239000011159 matrix material Substances 0.000 title claims abstract description 17
- 238000002360 preparation method Methods 0.000 title abstract description 8
- 238000000227 grinding Methods 0.000 claims abstract description 45
- 238000002791 soaking Methods 0.000 claims abstract description 38
- 239000004382 Amylase Substances 0.000 claims abstract description 27
- 102000013142 Amylases Human genes 0.000 claims abstract description 27
- 108010065511 Amylases Proteins 0.000 claims abstract description 27
- 102000004190 Enzymes Human genes 0.000 claims abstract description 27
- 108090000790 Enzymes Proteins 0.000 claims abstract description 27
- 235000019418 amylase Nutrition 0.000 claims abstract description 27
- 238000000034 method Methods 0.000 claims abstract description 27
- 230000001954 sterilising effect Effects 0.000 claims abstract description 19
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 19
- 238000010438 heat treatment Methods 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 230000009849 deactivation Effects 0.000 claims abstract description 12
- 238000005507 spraying Methods 0.000 claims abstract description 11
- 238000001238 wet grinding Methods 0.000 claims abstract description 8
- 238000001816 cooling Methods 0.000 claims abstract description 6
- 229940088598 enzyme Drugs 0.000 claims description 24
- 239000000758 substrate Substances 0.000 claims description 9
- 238000003801 milling Methods 0.000 claims description 8
- 108010059892 Cellulase Proteins 0.000 claims description 7
- 108091005804 Peptidases Proteins 0.000 claims description 7
- 239000004365 Protease Substances 0.000 claims description 7
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 7
- 229940106157 cellulase Drugs 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 5
- 239000000084 colloidal system Substances 0.000 claims description 2
- 239000007787 solid Substances 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 229920002498 Beta-glucan Polymers 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- -1 beta-glucan carotene Chemical class 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 235000013325 dietary fiber Nutrition 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 1
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
- 108010073771 Soybean Proteins Proteins 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 235000005473 carotenes Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 229920005862 polyol Polymers 0.000 description 1
- 150000003077 polyols Chemical class 0.000 description 1
- 238000004537 pulping Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000019710 soybean protein Nutrition 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000011345 viscous material Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
- A23L7/107—Addition or treatment with enzymes not combined with fermentation with microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C11/00—Other auxiliary devices or accessories specially adapted for grain mills
Abstract
The invention discloses a preparation method of a cereal thick slurry matrix, which comprises the following steps: soaking: soaking the grains according to a material-water ratio of 1: 2-1: 2.5, controlling the soaking temperature to be 50-60 ℃, adding the combined enzyme after soaking for 0.5-1 h, and continuing soaking for 1.5-2 h; wet dynamic grinding: putting the soaked grains into grinding equipment, adding water to carry out wet grinding, dynamically spraying amylase through an injector in the grinding process, and obtaining grain slurry after grinding is finished; enzymolysis: adding 0.05% of amylase into the slurry, heating the slurry to 55-60 ℃ for enzymolysis, wherein the enzymolysis time is 25-35 min, and heating the slurry to 90-95 ℃ for enzyme deactivation after the enzymolysis is finished; and (3) sterilization: and after enzyme deactivation, heating the pulp to 115-120 ℃ for sterilization for 15-18 min, and cooling the pulp to 5-10 ℃ after sterilization to obtain the cereal thick pulp matrix.
Description
Technical Field
The invention relates to the technical field of food manufacturing, in particular to a thick grain pulp matrix and a preparation method thereof.
Background
At present, in the industry, the product form taking grains as raw materials is mainly grain pulp, and the grain beverage prepared by using high-concentration grain pulp (solid content is 40-50%) as a substrate is used as a terminal consumer product, so that the use cost of the original pulp applied by an enterprise can be reduced, the production water is reduced, and the method is environment-friendly and effective.
The grains are rich in nutrients including dietary fiber, phenols, beta-glucan carotene, plant hormone, soybean protein, phytosterol, polyol, minerals and vitamins. In the preparation process of the grain pulp matrix, the common processes are soaking, high-strength mechanical grinding, enzymolysis, blending and sterilization of grains. However, due to the high cellulose content of the grain cortex, grinding efficiency is often compromised when grinding using conventional pulping processes because of the denser dry slurry state. In addition, the viscous material can damage the grinding equipment, creating high instantaneous temperatures that destroy the nutritional components of the grain.
The invention tries to prepare the grain thick slurry with 40 to 50 percent of higher solid concentration by the processes of enzyme method soaking wall breaking and dynamic amylase spraying, and meanwhile, the loose grain surface cortex structure formed in the enzyme method wall breaking process is beneficial to the dissolution of water-soluble protein, water-soluble dietary fiber and other water-soluble nutrient components in the subsequent processing.
Disclosure of Invention
In order to achieve the technical object, a first aspect of the present invention provides a method for preparing a cereal pulp matrix, which comprises the following steps:
a method of preparing a cereal pulp matrix comprising the steps of:
soaking: performing stirring type soaking on the grains according to a material-water ratio of 1: 2-1: 2.5, controlling the soaking temperature to be 50-60 ℃, adding the combined enzyme after soaking for 0.5-1 h, and continuing soaking for 1.5-2 h;
wet dynamic grinding: putting the soaked grains into grinding equipment, adding water to carry out wet grinding, dynamically spraying amylase through an injector in the grinding process, and obtaining grain slurry after grinding is finished;
enzymolysis: adding 0.05% of amylase into the slurry, heating the slurry to 55-60 ℃ for enzymolysis, wherein the enzymolysis time is 25-35 min, and heating the slurry to 90-95 ℃ for enzyme deactivation after the enzymolysis is finished;
and (3) sterilization: and after enzyme deactivation, heating the pulp to 115-120 ℃ for sterilization for 15-18 min, and cooling the pulp to 5-10 ℃ after sterilization to obtain the cereal thick pulp matrix.
In some embodiments, in the soaking step, the combined enzymes comprise: 0.1 to 0.2 percent of amylase, 0.1 to 0.15 percent of cellulase and 0.05 to 0.1 percent of protease.
In some embodiments, in the wet dynamic grinding step, the grinding temperature is 45-55 ℃.
In some embodiments, the spraying amount of the amylase in the wet dynamic grinding step is controlled to be 10-15 ml/5 min.
In some embodiments, in the wet dynamic grinding step, a high-pressure gas flow is dynamically sprayed through a high-pressure spray head during grinding.
In some embodiments, the milling apparatus is a colloid mill.
In a second aspect, the present invention provides a cereal pulp substrate made by the method of making the cereal pulp substrate of the first aspect of the invention.
The invention has the beneficial effects that:
1. the enzyme combination consisting of amylase, cellulase and protease is added in the soaking process, and the enzyme combination can realize effective release (enzymatic wall breaking) of grain cortex consisting of starch, protein and cellulose on the outer layer of grains, so that the grain cortex is more easily broken in the grinding stage, and the repeated dissolution of nutrients such as protein, beta-glucan and the like in the grains in the subsequent enzymolysis is ensured.
2. In the wet grinding process, amylase is dynamically sprayed by an injector to realize continuous degradation of starch in the grain slurry, so that the fluidity of the grain slurry is ensured under the condition of less water addition, and finally the grain thick slurry matrix with good fluidity and high solid concentration is obtained.
3. In the wet grinding process, high-pressure airflow is dynamically sprayed through a high-pressure nozzle, so that solid particles in the grain slurry are dispersed under the condition of less water adding amount, and the solid particles are prevented from being accumulated in the grinding equipment to damage the grinding equipment.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, preferred embodiments accompanied with figures are described in detail below, so as to clearly and clearly define the scope of the present invention.
Example one
The preparation method of the cereal thick slurry matrix in the embodiment comprises the following steps:
soaking: and (3) performing stirring type soaking on the grains according to the ratio of the material to the water of 1:2, controlling the soaking temperature to be 50 ℃, adding the combined enzyme after soaking for 0.5h, and continuing soaking for 2 h.
Wet dynamic grinding: and putting the soaked grains into grinding equipment, adding water to carry out wet grinding, dynamically spraying amylase through an injector in the grinding process, and obtaining grain slurry after grinding is finished.
Enzymolysis: adding 0.05% of amylase into the slurry, heating the slurry to 55 ℃ for enzymolysis, wherein the enzymolysis time is 35min, and heating the slurry to 90 ℃ for enzyme deactivation after the enzymolysis is finished;
and (3) sterilization: and after enzyme deactivation, heating the pulp to 115 ℃ for sterilization for 18min, and cooling the pulp to 5 ℃ after sterilization to obtain the cereal thick pulp matrix.
Wherein: in the soaking step, the combined enzyme comprises: 0.1% of amylase, 0.15% of cellulase and 0.05% of protease. In the wet dynamic grinding step, the grinding temperature is controlled to be 45 ℃, and the spraying amount of amylase is controlled to be 10ml/5 min. In addition, during the milling process, a high pressure jet is used to dynamically inject a high pressure gas stream into the grain slurry.
The cereal pulp substrate obtained in this example had solids content of about 40%.
Example two
The preparation method of the cereal thick slurry matrix in the embodiment comprises the following steps:
soaking: and (3) performing stirring type soaking on the grains according to the ratio of the material to the water of 1:2.5, controlling the soaking temperature to be 60 ℃, adding the combined enzyme after soaking for 1 hour, and continuing soaking for 1.5 hours.
Wet dynamic grinding: and putting the soaked grains into grinding equipment, adding water to carry out wet grinding, dynamically spraying amylase through an injector in the grinding process, and obtaining grain slurry after grinding is finished.
Enzymolysis: adding 0.05% of amylase into the slurry, heating the slurry to 60 ℃ for enzymolysis for 25min, and heating the slurry to 95 ℃ for enzyme deactivation after the enzymolysis is finished;
and (3) sterilization: and after enzyme deactivation, heating the pulp to 120 ℃ for sterilization for 15min, and cooling the pulp to 10 ℃ after sterilization to obtain the cereal thick pulp matrix.
Wherein: in the soaking step, the combined enzyme comprises: 0.2% of amylase, 0.1% of cellulase and 0.1% of protease. In the wet dynamic grinding step, the grinding temperature is controlled to be 55 ℃, and the spraying amount of amylase is controlled to be 15ml/5 min. In addition, during the milling process, a high pressure jet is used to dynamically inject a high pressure gas stream into the grain slurry.
The cereal pulp substrate obtained in this example had solids content of about 50%.
EXAMPLE III
The preparation method of the cereal thick slurry matrix in the embodiment comprises the following steps:
soaking: and (3) performing stirring type soaking on the grains according to the ratio of the material to the water of 1:2.2, controlling the soaking temperature to be 55 ℃, adding the combined enzyme after soaking for 0.8h, and continuing soaking for 1.8 h.
Wet dynamic grinding: and putting the soaked grains into grinding equipment, adding water to carry out wet grinding, dynamically spraying amylase through an injector in the grinding process, and obtaining grain slurry after grinding is finished.
Enzymolysis: adding 0.05% of amylase into the slurry, heating the slurry to 58 ℃ for enzymolysis, wherein the enzymolysis time is 30min, and heating the slurry to 93 ℃ for enzyme deactivation after the enzymolysis is finished;
and (3) sterilization: and after enzyme deactivation, heating the pulp to 118 ℃ for sterilization for 17min, and cooling the pulp to 8 ℃ after sterilization to obtain the cereal thick pulp matrix.
Wherein: in the soaking step, the combined enzyme comprises: 0.15% of amylase, 0.12% of cellulase and 0.08% of protease. In the wet dynamic grinding step, the grinding temperature is controlled at 50 ℃, and the spraying amount of amylase is controlled at 13ml/5 min. In addition, during the milling process, a high pressure jet is used to dynamically inject a high pressure gas stream into the grain slurry.
The cereal pulp substrate obtained in this example had solids content of about 43%.
It should be noted that, in the above three embodiments, 0.1 to 0.2% of amylase, 0.1 to 0.5% of cellulase, 0.05 to 0.1% of protease, and 0.05% of amylase are added during the soaking process, where the percentage values referred to refer to the percentage of the added components in the total mass of the slurry water or the mass of the slurry.
In addition, the cereal pulp substrate obtained in the three examples can be subjected to secondary grinding to obtain separation and other procedures so as to realize further deep processing.
The invention has been described above with a certain degree of particularity. It will be understood by those of ordinary skill in the art that the description of the embodiments is merely exemplary and that all changes that come within the true spirit and scope of the invention are desired to be protected. The scope of the invention is defined by the appended claims rather than by the foregoing description of the embodiments.
Claims (7)
1. A method of preparing a cereal pulp substrate, comprising the steps of:
soaking: performing stirring type soaking on the grains according to a material-water ratio of 1: 2-1: 2.5, controlling the soaking temperature to be 50-60 ℃, adding the combined enzyme after soaking for 0.5-1 h, and continuing soaking for 1.5-2 h;
wet dynamic grinding: putting the soaked grains into grinding equipment, adding water to carry out wet grinding, dynamically spraying amylase through an injector in the grinding process, and obtaining grain slurry after grinding is finished;
enzymolysis: adding 0.05% of amylase into the slurry, heating the slurry to 55-60 ℃ for enzymolysis, wherein the enzymolysis time is 25-35 min, and heating the slurry to 90-95 ℃ for enzyme deactivation after the enzymolysis is finished;
and (3) sterilization: and after enzyme deactivation, heating the pulp to 115-120 ℃ for sterilization for 15-18 min, and cooling the pulp to 5-10 ℃ after sterilization to obtain the cereal thick pulp matrix.
2. The method of preparing a cereal mash of claim 1 wherein in the steeping step, the combined enzymes comprise: 0.1 to 0.2 percent of amylase, 0.1 to 0.15 percent of cellulase and 0.05 to 0.1 percent of protease.
3. The method for preparing cereal thick slurry according to claim 1, wherein in the wet dynamic grinding step, the grinding temperature is 45-55 ℃.
4. The method for preparing cereal mash according to claim 1, wherein in the wet dynamic milling step, the amount of amylase sprayed is controlled to 10-15 ml/5 min.
5. The method for preparing a cereal mash according to claim 1 wherein in the wet dynamic milling step, a high pressure gas stream is dynamically injected through a high pressure nozzle during milling.
6. The method of preparing a cereal slurry of claim 1 wherein the milling device is a colloid mill.
7. A cereal pulp substrate prepared by the method of any of claims 1 to 6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011393788.9A CN112544869A (en) | 2020-12-03 | 2020-12-03 | Cereal thick slurry matrix and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011393788.9A CN112544869A (en) | 2020-12-03 | 2020-12-03 | Cereal thick slurry matrix and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
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| CN112544869A true CN112544869A (en) | 2021-03-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN202011393788.9A Pending CN112544869A (en) | 2020-12-03 | 2020-12-03 | Cereal thick slurry matrix and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114642244A (en) * | 2021-06-18 | 2022-06-21 | 内蒙古伊利实业集团股份有限公司 | Thick grain pulp and production method thereof |
| CN115486504A (en) * | 2021-06-18 | 2022-12-20 | 内蒙古伊利实业集团股份有限公司 | Oat grain thick pulp product and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5066218A (en) * | 1987-05-13 | 1991-11-19 | Genencor International, Inc. | Composition of a steeped starched-containing grain and a cellulase enzyme |
| CN107619842A (en) * | 2017-09-25 | 2018-01-23 | 山东神州翔宇科技集团有限公司 | A kind of method of Production by Enzymes cornstarch |
| CN111418760A (en) * | 2020-04-27 | 2020-07-17 | 安徽顺鑫盛源生物食品有限公司 | Preparation method of instant oat rice milk powder |
-
2020
- 2020-12-03 CN CN202011393788.9A patent/CN112544869A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5066218A (en) * | 1987-05-13 | 1991-11-19 | Genencor International, Inc. | Composition of a steeped starched-containing grain and a cellulase enzyme |
| CN107619842A (en) * | 2017-09-25 | 2018-01-23 | 山东神州翔宇科技集团有限公司 | A kind of method of Production by Enzymes cornstarch |
| CN111418760A (en) * | 2020-04-27 | 2020-07-17 | 安徽顺鑫盛源生物食品有限公司 | Preparation method of instant oat rice milk powder |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114642244A (en) * | 2021-06-18 | 2022-06-21 | 内蒙古伊利实业集团股份有限公司 | Thick grain pulp and production method thereof |
| CN115486504A (en) * | 2021-06-18 | 2022-12-20 | 内蒙古伊利实业集团股份有限公司 | Oat grain thick pulp product and preparation method thereof |
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Application publication date: 20210326 |