CN112538426B - Acoustic wave driven bubble resonance and micro-flow field excitation device and method - Google Patents
Acoustic wave driven bubble resonance and micro-flow field excitation device and method Download PDFInfo
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Abstract
The invention discloses a device and a method for exciting bubble resonance and a micro-flow field driven by sound waves, wherein the sound waves are generated by a piezoelectric transducer to excite the bubbles in a liquid pipe to oscillate, and the micro-flow field is generated by the micro-flow near the bubbles under the excitation of the oscillating bubbles so as to realize the purposes of liquid mixing, cell analysis and the like; compared with the existing micro flow field generated by excitation in the whole area of the liquid, the method avoids the blindness of operation and has stronger pertinence; compared with the existing method for generating the expected micro-flow field by utilizing the micro-fluidic chip through the directional flow formed by the liquid in the micro-flow chip, the method has higher flexibility; compared with the existing micro-column body excited by high-frequency vibration to generate a micro-flow field, the micro-flow field excitation device has stronger safety and relevance; in conclusion, the device and the method for the acoustic wave driven bubble resonance and micro-fluid field excitation effectively provided by the invention have great superiority and application potential in various kinds of micro-operations.
Description
Technical Field
The invention belongs to the technical field of micro-nano operation, and particularly relates to a device and a method for bubble resonance and micro-flow field excitation driven by sound waves.
Background
Along with the rapid development of the micro-nano technology, the progress of the micro-operation technology provides a very important tool for the fields of biomedicine, industrial production and the like. Among them, micro-fluid field excitation is becoming an important method of micromanipulation due to its characteristics of precision, high efficiency and no damage.
To date, the effective ways to achieve microfluidic field excitation in the field of micromanipulation are mainly divided into three types: firstly, the whole-region micro-flow field of the liquid environment of the operation object generates cavitation effect in the liquid by using ultrasonic waves, and generates a relatively violent micro-flow field near cavitation bubbles. The method has the characteristics that the local flow velocity of the generated micro-flow field is extremely high, the temperature can be raised to a certain degree, the action range comprises the whole operating liquid environment, no pertinence exists, the method is mainly suitable for some specific fields such as cell lysis and drug permeation, and the method has no universality. And secondly, the micro-fluidic chip is used for generating an expected micro-fluidic field by means of directional flow formed by liquid in the micro-fluidic chip, and the method can generate the expected and well-set micro-fluidic field, has feasibility of batch operation and is harmless to a target object. However, due to its closed structure, only specific operation tasks can be completed at a time, and the flexibility is poor, and it is difficult to adapt to some complex operations. The third way is to excite the micro-cylinders to vibrate at high frequency in specific positions and directions, and generate micro-flow fields by stirring the surrounding micro-fluid. The mode overcomes the problems of single function, poor adaptability and the like of the former two methods, but the high-frequency vibrating operation end has the possibility of mistakenly touching the target object, so the target object is damaged, and the whole vibration of the operation end often influences a non-operation object to bring inconvenience.
In summary, the existing method for micro-flow field excitation is increasingly difficult to meet the requirements of micro-nano operation technology.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a device and a method for resonance and micro-fluid field excitation of bubbles driven by sound waves. Multifunctional micro-operations such as liquid mixing, cell analysis and the like are realized by emitting sound waves into the liquid environment of the operation object, exciting micro-vortex fields generated around bubbles in a tube to generate resonance and generate micro-vortex fields around the bubbles. The problems of single function, poor flexibility, target object damage and the like in the micro-operation method at the present stage are effectively solved.
A device for bubble resonance and micro-flow field excitation driven by sound waves comprises a three-axis moving platform (1), a fine tuning platform (2), a micro glass tube (3), a PDMS condensate (4), an operating dish (6) and a piezoelectric transducer (8);
the fine adjustment platform (2) is fixed on the three-axis moving platform (1), and the three-axis moving platform (1) is used for adjusting the position of the fine adjustment platform (2);
the upper end of the micro glass tube (3) contains a PDMS condensate (4) with a set volume, the lower end of the micro glass tube is immersed in a liquid environment (7) of an operating dish (6), and bubbles (5) are generated at the lower end of the micro glass tube (3) under the action of surface tension;
the upper end of the micro glass tube (3) is fixed on the fine adjustment platform (2);
the piezoelectric transducer (8) is used for generating sound waves with set frequency and amplitude, so that the bubbles (7) are excited by the sound waves to periodically oscillate, and finally generate a uniform flow field and secondary radiation force around the bubbles.
Preferably, after the bubbles (5) are generated at the lower end of the micro glass tube (3) under the action of surface tension, part of gas of the bubbles (5) is extracted, and the gas-liquid interface of the bubbles is placed in the micro glass tube (3).
Preferably, the size of the bubbles (5) is adjusted by changing the volume of the PDMS condensate (4) and/or the inner diameter of the micro glass tube (3).
Preferably, the piezoelectric transducer (8) comprises a piezoelectric transducer sheet and a piezoelectric driver; the piezoelectric driver is used for inputting sinusoidal signals to the piezoelectric transduction piece, so that the piezoelectric transduction piece generates sound waves.
Preferably, the piezoelectric transducer (8) is bonded to the bottom of the operating dish (6).
An operation method of the device comprises the following operation steps:
sequentially dripping culture solutions with different components into a plurality of micro culture tanks on the culture dish (6), wherein the concentrations of the culture solutions with different components in each micro culture tank are also different;
adjusting a fine adjustment platform (2) of a triaxial movement platform (1), slowly immersing the micro glass tube (3) in liquid of an operating dish (6), and pumping out partial gas in the bubbles (5) by using a micro injector to enable a gas-liquid interface to be contracted in the micro glass tube (3);
opening a switch of the piezoelectric transducer (8), adjusting the frequency and amplitude of the output sound wave of the piezoelectric transducer, and when the frequency of the sound wave is close to the resonance frequency of the bubble (5), the bubble (5) obviously oscillates, so that a uniform micro eddy current field appears at the port of the micro glass tube (3);
under the excitation of sound waves emitted by the piezoelectric transducer (8), the bubbles (5) resonate, and a violent micro-vortex field is formed in the period of the bubbles, so that the culture solution is mixed in the micro-culture tank.
Preferably, the action time or oscillation intensity of the bubbles (5) in each micro-culture tank is controlled to produce different mixing effects.
An operation method of the device comprises the following operation steps:
placing single cells (12) in a plurality of cells in sequence in a cell support platform (11);
opening a switch of the piezoelectric transducer (8), adjusting the frequency and amplitude of the output sound wave of the piezoelectric transducer, and oscillating the bubble (5) when the frequency of the sound wave is close to the resonance frequency of the bubble (5), so that a uniform micro-vortex field appears at the port of the micro-glass tube (3);
the micro glass tube (3) is close to the central axis of each cell (12) in the direction vertical to the cell support platform (11), and the cell (12) deforms under the action of the flow field force; the input voltage of the piezoelectric transducer (8) is adjusted, the deformation condition of the cell (12) under different voltages is recorded, and the mechanical property of the cell (12) is calculated.
The invention has the following beneficial effects:
the invention provides a device and a method for exciting bubble resonance and a micro-flow field driven by sound waves, wherein the sound waves are generated by a piezoelectric transducer to excite the bubbles in a liquid pipe to oscillate, and the micro-flow field is generated by the micro-flow near the bubbles under the excitation of the oscillating bubbles so as to realize the purposes of liquid mixing, cell analysis and the like; compared with the existing micro flow field generated by excitation in the whole area of the liquid, the method avoids the blindness of operation and has stronger pertinence; compared with the existing method for generating the expected micro-flow field by utilizing the micro-fluidic chip through the directional flow formed by the liquid in the micro-flow chip, the method has higher flexibility; compared with the existing micro-flow field generated by exciting the micro-column body to vibrate at high frequency, the micro-flow field generator has stronger safety and relevance.
In conclusion, the device and the method for the acoustic wave driven bubble resonance and micro-fluid field excitation effectively provided by the invention have great superiority and application potential in various kinds of micro-operations.
Drawings
FIG. 1 is a schematic structural diagram of an acoustic wave driven bubble resonance and micro-fluid field excitation device according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of the micro-eddy current field distribution at the operation end of an acoustically-driven bubble resonance and micro-fluid field excitation device according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of an acoustically driven bubble resonance and micro-fluidic field excitation device for multiple concentration gradient liquid mixing, according to an embodiment of the present invention;
fig. 4 is a schematic diagram of an acoustic wave-driven bubble resonance and micro-fluid field excitation device for single cell mechanical analysis according to an embodiment of the present invention.
Detailed Description
In order to make the technical solutions better understood by those skilled in the art, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application.
Example one
The rapid and efficient achievement of uniform mixing of high viscosity liquids is very important in the fields of clinical diagnostics, chemical kinetics and material synthesis. Among them, in biomedicine, it is often necessary to adjust the chemical microenvironment around a single cell to study the response of the cell under the action of different concentrations. Taking this as a background, the present embodiment illustrates that the present invention can efficiently and flexibly mix a plurality of sets of liquids such as chemical reagents in a microfluidic environment by rapidly mixing multi-component liquids having different ratios.
Referring to fig. 1, there is shown a schematic diagram of an acoustic wave driven bubble resonance and micro-flow field excitation device for multi-concentration gradient liquid mixing according to an embodiment of the present invention; FIG. 3 is a schematic diagram of an acoustic wave driven bubble resonance and micro-fluid field excitation device for multi-concentration gradient liquid mixing according to an embodiment of the present invention.
A device for mixing multi-concentration gradient liquid comprises a three-axis moving platform 1, a fine adjustment platform 2, a micro glass tube 3, a PDMS condensate 4, a piezoelectric transducer 8 and a multi-groove culture dish 9; the fine adjustment platform 2 is fixed on the three-axis moving platform 1, wherein the three-axis moving platform 1 is used for adjusting the position of the fine adjustment platform 2; the micro glass tube 3 contains a PDMS condensate 4 with a certain length, and the other end of the micro glass tube is provided with air bubbles 5 and is placed in a multi-component culture solution 10 in a multi-groove culture dish 9; the piezoelectric transducer 8 is glued to the bottom of the multi-groove culture dish 9 by epoxy glue.
It should be noted that the three-axis mobile platform 1 may be fixed on a horizontal plane. Wherein the three-axis moving platform supports and roughly adjusts the working position of the whole operating device, for example, the air bubble 5 can be adjusted in the visual field of the microscope. The micro glass tube 3 is fixed on the fine adjustment platform 2, so that the fine adjustment platform 2 can be adjusted to realize the adjustment of the position and the pose of the bubbles 5 in the micro glass tube 3, for example, the micro glass tube can rotate within 360 degrees along the installation shaft of the micro glass tube on the three-axis movement platform 1, so as to adjust the distance and the angle between the bubbles 5 and the multi-groove culture dish 9 and the multi-component culture solution 10.
The micro glass tube 3 contains a PDMS condensate 4 with a certain length, one end which does not contain the PDMS condensate 4 is slowly immersed in the liquid of the multi-groove culture dish 9, air bubbles 5 can be generated at the port of the micro glass tube 3 under the action of surface tension, the size of the air bubbles 5 can be adjusted by properly changing the capacity of the PDMS condensate 4 and the inner diameter of the micro glass tube 3, and one end of the micro glass tube 3 containing the PDMS condensate 4 is fixed on the fine adjustment platform 2.
The piezoelectric transducer 8 comprises a piezoelectric transducer piece and a piezoelectric driver; the piezoelectric driver is used for inputting sinusoidal signals to the piezoelectric transduction piece, so that the piezoelectric transduction piece generates sound waves with certain frequency and amplitude.
It should be noted that the bubbles 5 periodically oscillate under the excitation of the sound waves generated by the piezoelectric transducer 8, so that a uniform micro-vortex field is generated around the bubbles to achieve the mixing of the multi-component culture solution 10.
It should be noted that the installation positions of the piezoelectric transducer and the piezoelectric actuator may be independent from each other, and the installation position of the piezoelectric actuator in the entire device is not limited in the embodiments of the present invention, as long as the piezoelectric actuator can provide a sinusoidal signal to the piezoelectric transducer, so that the piezoelectric transducer generates a sound wave with a certain frequency and amplitude.
Optionally, the inner diameter and the outer diameter of the micro glass tube 3 adopted in the embodiment of the invention are respectively 150 μm and 200 μm, the diameter of the bubble 5 is 150 μm, the length is 250 μm, and the distance between the gas-liquid interface and the port of the micro glass tube 3 is 40 μm; in other embodiments, the positions of the micro glass tube 3, the bubble 5 and the bubble 5 relative to the micro glass tube 3 may also adopt other sizes, and the description of this embodiment is omitted.
The working principle of the device for mixing the multi-concentration gradient liquid provided by the embodiment of the invention is as follows:
when a sinusoidal signal with certain frequency and amplitude is input into the piezoelectric transduction piece, the piezoelectric transduction piece emits sound waves with corresponding frequency and intensity. When the frequency of the sound wave is close to the resonance frequency of the bubble 5 in the micro glass tube 3, the bubble 5 can obviously oscillate under the excitation of the bubble, and the high-speed periodic oscillation can drive the liquid nearby the bubble to generate a time-averaged micro vortex field. Referring to fig. 2, which is a schematic diagram of the micro-eddy current field distribution at the operation end of an acoustic wave driven bubble resonance and micro-fluid field excitation device according to an embodiment of the present invention, two symmetrically distributed eddy currents are formed at the port of the micro glass tube 3.
Referring to fig. 3, a schematic diagram of an acoustic wave driven bubble resonance and micro-flow field excitation device for multi-concentration gradient liquid mixing according to an embodiment of the present invention is shown. A plurality of micro culture vessels are provided on the multi-well culture dish 9, and culture liquids of different compositions are dropped into the micro culture vessels in sequence, and the concentrations of the culture liquids of the compositions in each vessel are also different, thereby finally forming a multi-composition culture liquid 10. Under the excitation of the sound wave emitted by the piezoelectric transducer 8, the bubbles 5 will resonate, and form a violent micro-vortex field in the period to realize the mixing of the multi-component culture solution 10, and the action time or oscillation intensity of the bubbles 5 in each groove can be controlled to generate different mixing effects. The mixing of all the multi-component culture solution 10 in the multi-well culture dish 9 is completed in sequence, and then the cells are placed in each micro-culture tank to complete the relevant experiment.
Therefore, the device provided by the embodiment of the invention is composed of the three-axis moving platform 1, the fine adjustment platform 2, the micro glass tube 3, the PDMS condensate 4, the piezoelectric transducer 8 and the multi-groove culture dish 9, is simple in structure, can realize mixing of the multi-component culture solution 10 in different concentrations and different degrees only by providing power by the piezoelectric transducer 8, greatly improves the experimental efficiency of related operations, and avoids waste.
Example two
The method can quickly and effectively realize the mechanical property analysis of the single cell in an open environment, and has important application in the fields of cell research, tumor screening and the like. The present embodiment further illustrates the application of the acoustic wave-driven bubble resonance and micro-flow field excitation device and method in single cell analysis by detecting the mechanical properties of single cells.
Referring to fig. 1, there is shown a schematic diagram of an acoustically driven bubble resonance and micro-fluidic field excitation device for multiple concentration gradient liquid mixing, according to an embodiment of the present invention; fig. 4 is a schematic diagram of an acoustic wave driven bubble resonance and micro-flow field excitation device for single cell mechanical analysis according to an embodiment of the present invention.
A device for single cell mechanical analysis comprises a three-axis moving platform 1, a fine tuning platform 2, a micro glass tube 3, a PDMS condensate 4, a piezoelectric transducer 8 and a cell support platform 11; the fine adjustment platform 2 is fixed on the three-axis moving platform 1, wherein the three-axis moving platform 1 is used for adjusting the position of the fine adjustment platform 2; the micro glass tube 3 contains a PDMS condensate 4 with a certain length, and the other end of the micro glass tube is provided with air bubbles 5 and is placed in a culture solution in the cell support platform 11; the piezoelectric transducer 8 is glued to the bottom of the cell support platform 11 with epoxy glue.
It should be noted that the three-axis mobile platform 1 can be fixed on a horizontal plane. Wherein the three-axis moving platform supports and roughly adjusts the working position of the whole operating device, for example, the air bubble 5 can be adjusted in the visual field of the microscope. The micro glass tube 3 is fixed on the fine adjustment platform 2, so that the fine adjustment platform 2 can be adjusted to adjust the position and the pose of the bubble 5 in the micro glass tube 3, for example, the bubble 5 can rotate within 360 degrees along the installation shaft of the micro glass tube on the three-axis movement platform 1 to adjust the distance and the angle between the bubble 5 and the cell support platform 11 and the cell 12.
The micro glass tube 3 contains a PDMS condensate 4 with a certain length, one end without the PDMS condensate 4 is slowly immersed in the liquid of the cell support platform 11, bubbles 5 can be generated at the port of the micro glass tube 3 under the action of surface tension, the size of the bubbles 5 can be adjusted by properly changing the capacity of the PDMS condensate 4 and the inner diameter of the micro glass tube 3, and one end of the micro glass tube 3 containing the PDMS condensate 4 is fixed on the fine adjustment platform 2.
The piezoelectric transducer 8 comprises a piezoelectric transducer piece and a piezoelectric driver; the piezoelectric driver is used for inputting sinusoidal signals to the piezoelectric transduction piece, so that the piezoelectric transduction piece generates sound waves with certain frequency and amplitude.
It should be noted that the bubbles 5 periodically oscillate under the excitation of the sound wave generated by the piezoelectric transducer 8, so that when they are generated around them, they generate a uniform microflow field, and the directional force generated by this microflow field is applied to the direction of the symmetry axis of the cell 12, so as to implement the single cell mechanical analysis according to the deformation of the cell 12.
It should be noted that the installation positions of the piezoelectric transducer and the piezoelectric actuator may be independent from each other, and the installation position of the piezoelectric actuator in the entire device is not limited in the embodiments of the present invention, as long as the piezoelectric actuator can provide a sinusoidal signal to the piezoelectric transducer, so that the piezoelectric transducer generates a sound wave with a certain frequency and amplitude.
Optionally, the inner diameter and the outer diameter of the micro glass tube 3 adopted in the embodiment of the invention are respectively 50 μm and 100 μm, the diameter of the bubble 5 is 50 μm, the length is 150 μm, and the distance between the gas-liquid interface and the port of the micro glass tube 3 is 20 μm; in other embodiments, the positions of the micro glass tube 3, the bubble 5 and the bubble 5 relative to the micro glass tube 3 may also adopt other sizes, and the description of this embodiment is omitted.
The working principle of the device for mixing the multi-concentration gradient liquid provided by the embodiment of the invention is as follows:
when a sinusoidal signal with certain frequency and amplitude is input into the piezoelectric transduction piece, the piezoelectric transduction piece emits sound waves with corresponding frequency and intensity. When the frequency of the sound wave is close to the resonance frequency of the bubble 5 in the micro glass tube 3, the bubble 5 can obviously oscillate under the excitation of the bubble, and the high-speed periodic oscillation can drive the liquid nearby the bubble to generate a time-averaged microflow field. Referring to fig. 2, which is a schematic diagram of the micro-eddy current field distribution at the operation end of an acoustic wave driven bubble resonance and micro-fluid field excitation device according to an embodiment of the present invention, two symmetrically distributed eddy currents and a linear flow along the outside of the micro-glass tube 3 are formed at the port.
Referring to fig. 4, it is a schematic diagram of an acoustic wave driven bubble resonance and micro-flow field excitation device for single cell mechanical analysis according to an embodiment of the present invention. A plurality of linearly arranged tapered grooves are provided in the cell support platform 11, and the cells 12 are sequentially placed therein, and the kinds and sizes of the cells 12 may be different. Under the excitation of the sound wave emitted by the piezoelectric transducer 8, the bubbles 5 will resonate, and form a violent micro-flow field in the period, so that the micro-glass 3 approaches the central axis of each cell in the direction perpendicular to the cell support platform 11, and the cells will be immediately deformed under the action of the flow field force. The input voltage of the piezoelectric transducer 8 is adjusted, the deformation condition of the cell 12 under different voltages is recorded, and the mechanical property of the subcellular can be calculated through conversion.
Therefore, the device provided by the embodiment of the invention is composed of the three-axis moving platform 1, the fine adjustment platform 2, the micro glass tube 3, the PDMS condensate 4, the piezoelectric transducer 8 and the cell support platform 11, the structure is simple, the mechanical property tests of cells of different types and sizes in the cell support platform 11 can be realized only by providing power by the piezoelectric transducer 8, the experimental efficiency of related operations is greatly improved, and meanwhile, as the whole process is not in contact with the cells, the damage to the cells is reduced to the maximum extent, and the mechanical property of the cells is reflected more truly.
Although the embodiments of the present invention have been described in conjunction with the accompanying drawings, it will be apparent to those skilled in the art that various changes and modifications may be made therein without departing from the principles of the invention, and these are to be considered as falling within the scope of the invention.
Claims (8)
1. A device for bubble resonance and micro-flow field excitation driven by sound waves is characterized by comprising a three-axis moving platform (1), a fine tuning platform (2), a micro glass tube (3), a PDMS condensate (4), a multi-groove culture dish (9) and a piezoelectric transducer (8);
the fine adjustment platform (2) is fixed on the three-axis moving platform (1), and the three-axis moving platform (1) is used for adjusting the position of the fine adjustment platform (2);
the upper end of the micro glass tube (3) contains PDMS condensate (4) with a set volume, the lower end of the micro glass tube is immersed in a liquid environment (7) of a multi-groove culture dish (9), bubbles (5) are generated at the lower end of the micro glass tube (3) under the action of surface tension, and partial gas of the bubbles (5) is extracted to enable a gas-liquid interface of the bubbles to be arranged in the micro glass tube (3);
the upper end of the micro glass tube (3) is fixed on the fine adjustment platform (2);
the piezoelectric transducer (8) is used for generating sound waves with set frequency and amplitude, so that the bubbles (5) are excited by the sound waves to periodically oscillate, and finally generate a uniform flow field and secondary radiation force around the bubbles.
2. An acoustically driven bubble resonance and microflow field excitation device as claimed in claim 1, wherein after the bubble (5) is generated at the lower end of the micro glass tube (3) by surface tension, part of the gas of the bubble (5) is evacuated so that its gas-liquid interface is located inside the micro glass tube (3).
3. An acoustically driven bubble resonance and microfluidic field excitation device as claimed in claim 1, wherein the size of the bubbles (5) is adjusted by changing the volume of the PDMS solid (4) and/or the internal diameter of the microglass tube (3).
4. An acoustically driven bubble resonance and micro-fluidic field excitation device as claimed in claim 1, wherein said piezoelectric transducer (8) comprises a piezoelectric transducer plate and a piezoelectric actuator; the piezoelectric driver is used for inputting sinusoidal signals to the piezoelectric transduction piece, so that the piezoelectric transduction piece generates sound waves.
5. An acoustically driven bubble resonance and microflow field excitation device as claimed in claim 1, wherein said piezoelectric transducer (8) is bonded to the bottom of a multiwell dish (9).
6. An operation method based on the device of claim 1, characterized by comprising the following operation steps:
the culture solution with different components is sequentially dripped into a plurality of micro culture tanks on a multi-tank culture dish (9), and the concentration of the culture solution with different components in each micro culture tank is also different;
adjusting the three-axis moving platform (1) and the fine adjustment platform (2), slowly immersing the micro glass tube (3) in the liquid of the multi-tank culture dish (9), and pumping out part of gas in the bubbles (5) by using a micro injector to enable a gas-liquid interface to be contracted in the micro glass tube (3);
opening a switch of the piezoelectric transducer (8), adjusting the frequency and amplitude of the output sound wave of the piezoelectric transducer, and when the frequency of the sound wave is close to the resonance frequency of the bubble (5), the bubble (5) obviously oscillates, so that a uniform micro eddy current field appears at the port of the micro glass tube (3);
under the excitation of sound waves emitted by the piezoelectric transducer (8), the bubbles (5) resonate, and a violent micro-vortex field is formed in the period of the bubbles, so that the culture solution is mixed in the micro-culture tank.
7. Operating method according to claim 6, characterized in that the action time or the intensity of the oscillation of the bubbles (5) in each micro-culture tank is controlled to produce different mixing effects.
8. An operation method based on the device of claim 1, characterized by comprising the following operation steps:
placing single cells (12) in a plurality of cells in sequence in a cell support platform (11);
opening a switch of the piezoelectric transducer (8), adjusting the frequency and amplitude of the output sound wave of the piezoelectric transducer, and oscillating the bubble (5) when the frequency of the sound wave is close to the resonance frequency of the bubble (5), so that a uniform micro-vortex field appears at the port of the micro-glass tube (3);
the micro glass tube (3) is close to the central axis of each cell (12) in the direction vertical to the cell support platform (11), and the cell (12) deforms under the action of the flow field force; the input voltage of the piezoelectric transducer (8) is adjusted, the deformation condition of the cell (12) under different voltages is recorded, and the mechanical property of the cell (12) is calculated.
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