CN112522101A

CN112522101A - Liquid supplementing device for cell culture

Info

Publication number: CN112522101A
Application number: CN202011429401.0A
Authority: CN
Inventors: 杨慧慧
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-12-07
Filing date: 2020-12-07
Publication date: 2021-03-19

Abstract

The invention discloses a liquid supplementing device for cell culture, which belongs to the technical field of cell culture and comprises a workbench, a bearing frame, an injection device, a fixing device, a stirring device, a closing device, a mounting device, a self-locking device and a culture cylinder, wherein the bearing frame is arranged at the top of the workbench, an opening is formed in the bearing frame, the fixing device is arranged at the top of the workbench and is in sliding fit with the workbench, the injection device is arranged at the top of the workbench, the stirring device is arranged at the top of the bearing frame and is in rotating fit with the bearing frame, the closing device is arranged on the injection device, the mounting device is arranged on the injection device, the culture cylinder is arranged beside the workbench, and the self-locking device is arranged at the top of the culture cylinder. According to the cell culture fluid supplementing device, the culture fluid is stirred uniformly in advance, the mounting device is inserted into the culture cylinder to prevent external bacteria from contacting cells, and then the culture fluid is quantitatively supplemented.

Description

Liquid supplementing device for cell culture

Technical Field

The invention relates to the technical field of cell culture, in particular to a liquid supplementing device for cell culture.

Background

In large scale in vitro cell expansion culture, a large amount of fresh culture medium is often required to be supplemented to meet the nutrient components necessary for cell growth, and the cell supplement amount is low.

The utility model discloses a cell culture fluid infusion device of CN209636248U now, the utility model provides a cell culture fluid infusion device, including hollow cylinder, the hollow cylinder is inside to be equipped with the piston, piston upper end installation cylinder push rod, cylinder push rod annular outer end suit gravity weight, the gravity weight is installed in the piston upper end, hollow cylinder lower extreme intermediate position fixes the drain pipe, install check valve one on the drain pipe, drain pipe lower extreme installation joint just extends to the joint inside, the joint rear end is equipped with the back supporting cloth, back supporting cloth front end fixed front supporting cloth, back supporting cloth upper end installation strap, the strap is fixed at hollow cylinder lower extreme, hollow cylinder lower extreme right part position fixed feed liquor pipe, the last check valve two of installing of feed liquor pipe, this design has reduced the pollution probability, and the utility model is rational in infrastructure, the smoothness of fluid infusion operation is improved, and the operation difficulty is reduced.

Above-mentioned utility model when carrying out the fluid infusion, the degree of consistency of nutrient solution is not high, can disturb cell culture, does not send out at the in-process culture solution that carries out the fluid infusion simultaneously and carries out quantitative transport, and the culture solution is too much and can the direct interference cell culture too little.

Disclosure of Invention

The invention aims to provide a liquid supplementing device for cell culture, so as to solve the technical problem.

In order to solve the technical problems, the invention provides the following technical scheme: the utility model provides a fluid infusion device for cell culture, includes the workstation, bears frame, injection apparatus, fixing device, agitating unit, closing device, installation device, self-lock device and culture section of thick bamboo, bear the top that the frame set up at the workstation, bear and be equipped with the opening on the frame, fixing device set up at the top of workstation and with workstation sliding fit, injection apparatus sets up the top at the workstation, agitating unit set up at the top of bearing the frame and with bear a normal running fit, closing device sets up on injection apparatus, installation device sets up on injection apparatus, the culture section of thick bamboo sets up the side at the workstation, self-lock device sets up the top at the culture section of thick bamboo.

Further, the injection device includes injection electricity jar, injection pole, injection sealed dish and injection tube, the injection tube sets up the top at the workstation, be equipped with the sprue on the injection tube, the injection electricity jar sets up the top at the workstation, the flexible of injection electricity jar is connected to the one end of injection pole and is served, the injection sealed dish sets up in the injection tube, be equipped with one deck rubber sleeve on the injection sealed dish, be equipped with a plurality of airtight lamellas on the injection sealed dish, the injection sealed dish is connected on the other end of injection pole.

Further, fixing device includes two fixed subassemblies, two the fixed subassembly symmetry sets up on the workstation and is located the both sides of injection tube, two fixed subassembly all includes fixed motor, fixed gear, fixed pinion rack and mount, the mount setting at the top of workstation and with workstation sliding fit, fixed pinion rack sets up on the mount, fixed gear rotates and sets up on the workstation, fixed gear meshes with fixed pinion rack mutually, fixed motor's main shaft is connected on fixed gear.

Further, the stirring device comprises a worm wheel, a worm, a stirring cylinder, a rotating motor, a bearing plate, a stirring motor, a stirring rotating rod, a stirring connecting rod, a supporting rod and a stirring rod, wherein the worm wheel is rotatably arranged at the top of the bearing frame and positioned at an opening, the worm is rotatably arranged on the bearing frame, the worm is meshed with the worm wheel, the rotating motor is arranged on the bearing frame, a main shaft of the rotating motor is connected to the worm, the stirring cylinder is arranged on the worm wheel, the bearing plate is arranged at the top of the bearing frame, the stirring rotating rod is rotatably arranged on the bearing plate, the stirring motor is arranged on the bearing plate, the main shaft of the stirring motor penetrates through the bearing plate and is connected to the stirring rotating rod, one end of the stirring connecting rod is hinged to the stirring rotating rod, one end of the supporting rod is connected to the bearing plate, the stirring rod is hinged at the other end of the supporting rod.

Furthermore, the closing device comprises a feeding barrel, a bottom barrel, a rotating ring, a rotating gear, a closing motor and a plurality of closing arc plates, the bottom barrel is arranged on the injection barrel, the rotating ring is rotatably arranged on the bottom barrel, the rotating ring is provided with a plurality of tooth blocks, the rotating ring is provided with a plurality of sliding grooves, the closing motor is arranged on the bearing frame, the rotating gear is connected to a main shaft of the closing motor and meshed with the tooth blocks on the rotating ring, the closing arc plates are hinged to the bottom barrel and the sliding grooves, and the feeding barrel is arranged at the top of the rotating ring and below the opening.

Further, the installation device comprises an installation hose, an installation ring and four installation assemblies, one end of the installation hose is connected to the discharge end of the injection tube, the installation ring is arranged at the other end of the installation hose, the installation assemblies are arranged around the installation ring, the installation assemblies comprise a moving block, an installation gear, a sliding arc plate and two installation springs, the moving block is arranged at the top of the installation ring and is in sliding fit with the installation ring, a fixing groove is formed in one end of the moving block, the two ends of the installation springs are connected to the moving block and the installation ring respectively, the installation gear is arranged on the installation ring in a rotating mode, the sliding arc plate is arranged at the bottom of the installation ring in a sliding mode, and tooth grooves are formed in the moving block and the sliding arc plate and are meshed with the installation gear.

Further, self-lock device is including an installation section of thick bamboo, four spacing tracks, four auto-lock pieces and four auto-lock springs, an installation section of thick bamboo sets up the top at the culture section of thick bamboo, be equipped with the bell jar on the installation section of thick bamboo, four spacing tracks all set up on the bell jar, four the auto-lock piece all set up on the installation section of thick bamboo and with installation section of thick bamboo sliding fit, four the both ends of auto-lock spring are connected on an installation section of thick bamboo and four auto-lock pieces.

Compared with the prior art, the invention has the beneficial effects that:

firstly, when the culture cylinder needs to be replenished with liquid, the injection electric cylinder starts to operate to drive the injection rod to start moving, the injection rod moves to drive the injection sealing disc to move in the injection tube, and the injection sealing disc pushes the plurality of airtight flaps inwards to close, so that the culture liquid in the injection tube is pushed into the mounting device to be gradually conveyed, and the culture liquid is quantitatively conveyed to the culture cylinder.

Secondly, before fluid infusion is started, culture fluid is injected into the mixing drum, then the motor is rotated to start to move, the worm is driven to rotate, the worm rotates to drive the worm wheel meshed with the worm to rotate, the worm wheel rotates to drive the mixing drum to start to rotate, meanwhile, the mixing motor starts to rotate to drive the mixing rotating rod to start to rotate, the mixing rotating rod rotates to drive the mixing connecting rod to move, the mixing connecting rod moves to drive the mixing rod to rotate on the supporting rod, the mixing rod is matched with the mixing drum, the culture fluid is uniformly mixed, and the phenomenon that the culture fluid is not uniform and cell culture is not uniform is avoided.

Thirdly, before fluid infusion begins, the mounting ring is inserted into the self-locking device, the four moving blocks are pushed to extrude the mounting springs to retract under the action of the self-locking device, the four moving blocks retract to drive the four mounting gears to rotate, the four mounting gears rotate to drive the four sliding arc plates to move, the mounting ring is opened, then the four moving blocks are fixed by the self-locking device, and the mounting device is inserted into the self-locking device to prevent cells from contacting the outside so as to avoid interference of external factors on cell culture.

Fourthly, before work starts, the mounting ring enters the tapered groove, the four moving blocks move in the four limiting tracks, when the four moving blocks reach the four self-locking block positions, the four moving blocks extrude the four self-locking blocks to retract, then when the four moving blocks move the four fixed grooves to the four self-locking blocks, the self-locking blocks pop up to fix the moving blocks, and the mounting ring is prevented from popping up due to the elasticity of the mounting spring so as to carry out liquid supplementing operation.

Drawings

FIG. 1 is a first perspective view of the present invention;

FIG. 2 is a schematic perspective view of the present invention;

FIG. 3 is a schematic perspective view of the injection device of the present invention;

FIG. 4 is a perspective view of the fixing device of the present invention;

FIG. 5 is a schematic perspective view of a stirring device according to the present invention;

FIG. 6 is a schematic perspective view of a closure device of the present invention;

FIG. 7 is a schematic perspective view of the mounting device of the present invention;

FIG. 8 is a perspective view of the mounting assembly of the present invention;

fig. 9 is a schematic perspective view of the self-locking device of the present invention.

The reference numbers in the figures are:

the device comprises a workbench 1, a bearing frame 11, an injection device 2, a fixing device 3, a stirring device 4, a closing device 5, a mounting device 6, a self-locking device 7, a culture cylinder 12, an injection electric cylinder 22, an injection rod 23, an injection sealing disc 24, an injection tube 25, an air tight flap 26, a fixing component 31, a fixing motor 32, a fixed gear 33, a fixed toothed plate 34, a fixing frame 35, a worm wheel 41, a worm 42, a stirring cylinder 43, a rotating motor 44, a bearing plate 45, a stirring motor 46, a stirring rotating rod 47, a stirring connecting rod 48, a supporting rod 49, a stirring rod 491, a discharging cylinder 51, a bottom cylinder 52, a rotating ring 53, a rotating gear 54, a closing motor 55, a closing arc plate 56, a toothed block 57, a mounting hose 61, a mounting ring 62, a mounting component 63, a moving block 64, a mounting gear 65, a sliding arc plate 66, a mounting spring 67, a limiting rail 71, a self-locking block.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

Referring to fig. 1 to 9, the present invention provides a fluid infusion device for cell culture, including a workbench 1, a carrier 11, an injection device 2, a fixing device 3, a stirring device 4, a closing device 5, a mounting device 6, a self-locking device 7 and a culture cylinder 12, wherein the carrier 11 is disposed on the top of the workbench 1, the carrier 11 is provided with an opening, the fixing device 3 is disposed on the top of the workbench 1 and is in sliding fit with the workbench 1, the injection device 2 is disposed on the top of the workbench 1, the stirring device 4 is disposed on the top of the carrier 11 and is in rotating fit with the carrier 11, the closing device 5 is disposed on the injection device 2, the mounting device 6 is disposed on the injection device 2, the culture cylinder 12 is disposed beside the workbench 1, and the self-locking device 7 is disposed on the top of the culture cylinder 12.

The injection device 2 comprises an injection electric cylinder 22, an injection rod 23, an injection sealing disc 24 and an injection tube 25, wherein the injection tube 25 is arranged at the top of the workbench 1, a feeding port is arranged on the injection tube 25, the injection electric cylinder 22 is arranged at the top of the workbench 1, one end of the injection rod 23 is connected to the telescopic end of the injection electric cylinder 22, the injection sealing disc 24 is arranged in the injection tube 25, a layer of rubber sleeve is arranged on the injection sealing disc 24, a plurality of airtight flaps 26 are arranged on the injection sealing disc 24, and the injection sealing disc 24 is connected to the other end of the injection rod 23; when liquid needs to be replenished into the culture cylinder 12, the injection electric cylinder 22 starts to operate to drive the injection rod 23 to move, the injection rod 23 moves to drive the injection sealing disc 24 to move in the injection tube 25, and the injection sealing disc 24 pushes a plurality of airtight flaps 26 inwards to close, so that the culture liquid in the injection tube 25 is pushed to enter the mounting device 6 to be gradually conveyed, and the culture liquid is quantitatively conveyed to the culture cylinder 12.

The fixing device 3 comprises two fixing assemblies 31, the two fixing assemblies 31 are symmetrically arranged on the workbench 1 and located on two sides of the injection tube 25, each fixing assembly 31 comprises a fixing motor 32, a fixed gear 33, a fixed toothed plate 34 and a fixing frame 35, the fixing frame 35 is arranged at the top of the workbench 1 and is in sliding fit with the workbench 1, the fixed toothed plate 34 is arranged on the fixing frame 35, the fixed gear 33 is rotatably arranged on the workbench 1, the fixed gear 33 is meshed with the fixed toothed plate 34, and a main shaft of the fixing motor 32 is connected to the fixed gear 33; before the work begins, two fixed motor 32 begin to operate, drive two fixed gear 33 and begin to rotate, and two fixed gear 33 rotate and drive two fixed tooth plate 34 and begin to remove, and two fixed tooth plate 34 drive two mount 35 and press from both sides tight fixedly to injection tube 25, prevent at the fluid infusion in-process, injection tube 25 produces the slip, interference device's fluid infusion efficiency.

Agitating unit 4 includes worm wheel 41, worm 42, churn 43, rotates motor 44, loading board 45, agitator motor 46, stirring dwang 47, stirring connecting rod 48, bracing piece 49 and puddler 491, worm wheel 41 rotates and sets up at the top of bearing frame 11 and is located the opening part, worm 42 rotates and sets up on bearing frame 11, worm 42 meshes with worm wheel 41 mutually, it sets up on bearing frame 11 and the main shaft of rotating motor 44 connects on worm 42 to rotate motor 44, churn 43 sets up on worm wheel 41, loading board 45 sets up the top at bearing frame 11, stirring dwang 47 rotates and sets up on loading board 45, agitator motor 46 sets up on loading board 45 and agitator motor 46's main shaft runs through loading board 45 and connects on stirring dwang 47, the one end of stirring connecting rod 48 articulates on stirring dwang 47, one end of the supporting rod 49 is connected to the bearing plate 45, one end of the stirring rod 491 is hinged to the stirring connecting rod 48, and the stirring rod 491 is hinged to the other end of the supporting rod 49; before fluid infusion begins, culture fluid is injected into the stirring cylinder 43, then the rotating motor 44 starts to move, the worm 42 is driven to rotate, the worm 42 rotates to drive the worm wheel 41 meshed with the worm 42 to rotate, the worm wheel 41 rotates to drive the stirring cylinder 43 to rotate, meanwhile, the stirring motor 46 starts to rotate to drive the stirring rotating rod 47 to rotate, the stirring rotating rod 47 rotates to drive the stirring connecting rod 48 to move, the stirring connecting rod 48 moves to drive the stirring rod 491 to rotate on the supporting rod 49, the stirring connecting rod 48 is matched with the stirring cylinder 43 to stir the culture fluid uniformly, and the culture fluid is prevented from being non-uniform, so that the cell culture is not uniform.

The closing device 5 comprises a feeding barrel 51, a bottom barrel 52, a rotating ring 53, a rotating gear 54, a closing motor 55 and a plurality of closing arc plates 56, the bottom barrel 52 is arranged on the injection cylinder 25, the rotating ring 53 is rotatably arranged on the bottom barrel 52, the rotating ring 53 is provided with a plurality of tooth blocks 57, the rotating ring 53 is provided with a plurality of sliding grooves, the closing motor 55 is arranged on the bearing frame 11, the rotating gear 54 is connected on a main shaft of the closing motor 55 and meshed with the tooth blocks 57 on the rotating ring 53, the plurality of closing arc plates 56 are hinged on the bottom barrel 52 and the plurality of sliding grooves, and the feeding barrel 51 is arranged at the top of the rotating ring 53 and is positioned below the opening; when need inject the culture solution into injection tube 25, closing motor 55 begins to operate, drives rotating gear 54 and begins to rotate, and rotating gear 54 drives swivel 53 and begins to rotate to drive a plurality of closed arc boards 56 and rotate through removing in a plurality of sliding grooves, then the culture solution gets into end section of thick bamboo 52 through a plurality of closed arc boards 56 from feed cylinder 51, thereby in injecting injection tube 25, cooperate with injection device 2 and carry out quantitative fluid infusion to the culture solution.

The mounting device 6 comprises a mounting hose 61, a mounting ring 62 and four mounting assemblies 63, one end of the mounting hose 61 is connected with the discharge end of the injection cylinder 25, the mounting ring 62 is arranged at the other end of the mounting hose 61, four mounting assemblies 63 are arranged around the mounting ring 62, each of the four mounting assemblies 63 comprises a moving block 64, a mounting gear 65, a sliding arc plate 66 and two mounting springs 67, the moving block 64 is arranged on the top of the mounting ring 62 and is in sliding fit with the mounting ring 62, one end of the moving block 64 is provided with a fixing groove, two ends of the two mounting springs 67 are respectively connected to the moving block 64 and the mounting ring 62, the mounting gear 65 is rotatably arranged on the mounting ring 62, the sliding arc plate 66 is slidably arranged at the bottom of the mounting ring 62, tooth grooves are formed in the moving block 64 and the sliding arc plate 66 and are meshed with the mounting gear 65; before fluid infusion begins, the mounting ring 62 is inserted into the self-locking device 7, under the action of the self-locking device 7, the four moving blocks 64 are pushed to extrude the mounting springs 67 to retract, the four moving blocks 64 retract to drive the four mounting gears 65 to rotate, the four mounting gears 65 rotate to drive the four sliding arc plates 66 to move, the mounting ring 62 is opened, then the four moving blocks 64 are fixed by the self-locking device 7, the mounting device 6 is inserted into the self-locking device 7, cell contact is prevented from the outside, and the cell culture is prevented from being interfered by external factors.

The self-locking device 7 comprises an installation cylinder 74, four limiting rails 71, four self-locking blocks 72 and four self-locking springs 73, wherein the installation cylinder 74 is arranged at the top of the culture cylinder 12, a tapered groove is formed in the installation cylinder 74, the four limiting rails 71 are arranged on the tapered groove, the four self-locking blocks 72 are arranged on the installation cylinder 74 and are in sliding fit with the installation cylinder 74, and two ends of the four self-locking springs 73 are connected to the installation cylinder 74 and the four self-locking blocks 72; before work begins, the mounting ring 62 enters the tapered groove, the four moving blocks 64 move in the four limiting tracks 71, when the four moving blocks 64 reach the positions of the four self-locking blocks 72, the four moving blocks 64 extrude the four self-locking blocks 72 to retract, then when the four moving blocks 64 move the four fixing grooves to the positions of the four self-locking blocks 72, the self-locking blocks 72 pop up to fix the moving blocks 64, and the situation that the mounting ring 62 pops up due to the elasticity of the mounting springs 67 is prevented so that liquid supplementing operation can be conducted.

The working principle of the invention is as follows: before the work begins, the two fixed motors 32 start to operate to drive the two fixed gears 33 to start rotating, the two fixed gears 33 rotate to drive the two fixed toothed plates 34 to start moving, the two fixed toothed plates 34 drive the two fixed frames 35 to clamp and fix the injection tube 25, so that the injection tube 25 is prevented from sliding in the fluid infusion process, the fluid infusion efficiency of the device is prevented from being interfered, before fluid infusion begins, the mounting ring 62 is inserted into the self-locking device 7, under the action of the self-locking device 7, the four moving blocks 64 are pushed to extrude the mounting springs 67 to retract, the four moving blocks 64 retract to drive the four mounting gears 65 to rotate, the four mounting gears 65 rotate to drive the four sliding arc plates 66 to move, the mounting ring 62 is opened, then the self-locking device 7 fixes the four moving blocks 64, the mounting device 6 is inserted into the self-locking device 7, cells are prevented from contacting the outside, and the culture of, before work begins, the mounting ring 62 enters the conical groove, the four moving blocks 64 move in the four limiting tracks 71, when the four moving blocks 64 reach the four self-locking blocks 72, the four moving blocks 64 press the four self-locking blocks 72 to retract, then when the four moving blocks 64 move the four fixing grooves to the four self-locking blocks 72, the four self-locking blocks 72 pop out to fix the moving blocks 64, so that the situation that the mounting ring 62 pops out due to the elasticity of the mounting spring 67 is prevented to facilitate fluid infusion operation, before fluid infusion begins, culture fluid is injected into the stirring barrel 43, then the rotating motor 44 begins to move to drive the worm 42 to rotate, the worm 42 rotates to drive the worm wheel 41 which is meshed with the worm wheel to rotate, the worm wheel 41 rotates to drive the stirring barrel 43 to rotate, the stirring motor 46 simultaneously rotates to drive the stirring rotating rod 47 to rotate, the stirring rotating rod 47 rotates to drive the stirring connecting rod 48 to move, the stirring connecting rod 48 moves to drive the stirring rod 491 to rotate on the supporting rod 49 and cooperate with the stirring cylinder 43 to stir the culture fluid uniformly, so as to prevent the culture fluid from being non-uniform and cause the cell culture to be non-uniform, when the culture fluid is required to be injected into the injection cylinder 25, the closed motor 55 starts to operate to drive the rotating gear 54 to rotate, the rotating gear 54 drives the rotating ring 53 to rotate, thereby driving the plurality of closed arc plates 56 to rotate by moving in the plurality of sliding grooves, then the culture fluid enters the injection cylinder bottom 52 from the feed cylinder 51 through the plurality of closed arc plates 56 to be injected into the injection cylinder 25 and cooperate with the injection device 2 to carry out quantitative fluid infusion on the culture fluid, when the fluid infusion needs to be carried out into the culture cylinder 12, the injection electric cylinder 22 starts to operate to drive the injection rod 23 to move, the injection rod 23 moves to drive the injection sealing disc 24 to move in the injection, the injection sealing disk 24 is pushed inward to press the airtight flaps 26 closed, thereby pushing the culture solution in the injection tube 25 into the mounting device 6 for stepwise delivery and quantitative delivery of the culture solution to the culture container 12.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A fluid infusion device for cell culture is characterized in that: comprises a workbench (1), a bearing frame (11), an injection device (2), a fixing device (3), a stirring device (4), a closing device (5), a mounting device (6), a self-locking device (7) and a culture cylinder (12), wherein the bearing frame (11) is arranged at the top of the workbench (1), the bearing frame (11) is provided with an opening, the fixing device (3) is arranged at the top of the workbench (1) and is in sliding fit with the workbench (1), the injection device (2) is arranged at the top of the workbench (1), the stirring device (4) is arranged at the top of the bearing frame (11) and is in rotating fit with the bearing frame (11), the closing device (5) is arranged on the injection device (2), the mounting device (6) is arranged on the injection device (2), the culture cylinder (12) is arranged beside the workbench (1), the self-locking device (7) is arranged at the top of the culture cylinder (12).

2. The fluid infusion device for cell culture according to claim 1, wherein: injection device (2) is including injection electricity jar (22), injection pole (23), injection sealed dish (24) and injection tube (25), injection tube (25) set up the top in workstation (1), be equipped with the sprue on injection tube (25), injection electricity jar (22) set up the top at workstation (1), the one end of injection pole (23) is connected and is served in the flexible of injection electricity jar (22), injection sealed dish (24) set up in injection tube (25), be equipped with one deck rubber sleeve on injection sealed dish (24), be equipped with a plurality of airtight lamella (26) on injection sealed dish (24), injection sealed dish (24) are connected on the other end of injection pole (23).

3. The fluid infusion device for cell culture according to claim 2, wherein: fixing device (3) include two fixed subassembly (31), two fixed subassembly (31) symmetry sets up on workstation (1) and is located the both sides of injection tube (25), two fixed subassembly (31) all include fixed motor (32), fixed gear (33), fixed pinion rack (34) and mount (35), mount (35) set up at the top of workstation (1) and with workstation (1) sliding fit, fixed pinion rack (34) set up on mount (35), fixed gear (33) rotate and set up on workstation (1), fixed gear (33) mesh with fixed pinion rack (34), the main shaft of fixed motor (32) is connected on fixed gear (33).

4. The fluid infusion device for cell culture according to claim 1, wherein: agitating unit (4) include worm wheel (41), worm (42), churn (43), rotate motor (44), loading board (45), agitator motor (46), stirring dwang (47), stirring connecting rod (48), bracing piece (49) and puddler (491), worm wheel (41) rotate to set up and just be located the opening part at the top of bearing frame (11), worm (42) rotate to set up on bearing frame (11), worm (42) mesh with worm wheel (41) mutually, rotate motor (44) and set up on bearing frame (11) and the main shaft of rotating motor (44) is connected on worm (42), churn (43) set up on worm wheel (41), loading board (45) set up the top of bearing frame (11), stirring dwang (47) rotate to set up on loading board (45), agitator motor (46) set up on loading board (45) and agitator motor (46)'s main shaft runs through loading board (45) ) And connect on stirring dwang (47), the one end of stirring connecting rod (48) articulates on stirring dwang (47), on loading board (45) is connected to the one end of bracing piece (49), the one end of puddler (491) articulates on stirring connecting rod (48), puddler (491) articulates the other end at bracing piece (49).

5. The fluid infusion device for cell culture according to claim 2, wherein: the closing device (5) comprises a feeding barrel (51), a bottom barrel (52), a rotating ring (53), a rotating gear (54), a closing motor (55) and a plurality of closing arc plates (56), the bottom barrel (52) is arranged on the injection barrel (25), the rotating ring (53) is rotatably arranged on the bottom barrel (52), a plurality of tooth blocks (57) are arranged on the rotating ring (53), a plurality of sliding grooves are formed in the rotating ring (53), the closing motor (55) is arranged on the bearing frame (11), the rotating gear (54) is connected to a main shaft of the closing motor (55) and meshed with the tooth blocks (57) on the rotating ring (53), the closing arc plates (56) are hinged to the bottom barrel (52) and the sliding grooves, and the feeding barrel (51) is arranged at the top of the rotating ring (53) and is located below the opening.

6. The fluid infusion device for cell culture according to claim 2, wherein: the mounting device (6) comprises a mounting hose (61), a mounting ring (62) and four mounting assemblies (63), one end of the mounting hose (61) is connected to the discharge end of the injection cylinder (25), the mounting ring (62) is arranged at the other end of the mounting hose (61), the four mounting assemblies (63) are arranged around the mounting ring (62), each of the four mounting assemblies (63) comprises a moving block (64), a mounting gear (65), a sliding arc plate (66) and two mounting springs (67), the moving block (64) is arranged at the top of the mounting ring (62) and is in sliding fit with the mounting ring (62), one end of the moving block (64) is provided with a fixing groove, two ends of the two mounting springs (67) are respectively connected to the moving block (64) and the mounting ring (62), the mounting gear (65) is rotatably arranged on the mounting ring (62), the sliding arc plate (66) is arranged at the bottom of the mounting ring (62) in a sliding mode, and the moving block (64) and the sliding arc plate (66) are both provided with tooth grooves and are both meshed with the mounting gear (65).

7. The fluid infusion device for cell culture according to claim 1, wherein: self-lock device (7) are including installation section of thick bamboo (74), four spacing track (71), four from locking piece (72) and four auto-lock spring (73), installation section of thick bamboo (74) set up the top at culture section of thick bamboo (12), be equipped with the bell jar on installation section of thick bamboo (74), four spacing track (71) all set up on the bell jar, four from locking piece (72) all set up on installation section of thick bamboo (74) and with installation section of thick bamboo (74) sliding fit, four the both ends of auto-lock spring (73) are connected on installation section of thick bamboo (74) and four auto-lock piece (72).