CN112522076A - Kit for identifying authenticity of ginseng - Google Patents
Kit for identifying authenticity of ginseng Download PDFInfo
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- CN112522076A CN112522076A CN202011441541.XA CN202011441541A CN112522076A CN 112522076 A CN112522076 A CN 112522076A CN 202011441541 A CN202011441541 A CN 202011441541A CN 112522076 A CN112522076 A CN 112522076A
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- extraction tank
- medicament bottle
- connecting pipe
- ginseng
- tank
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- 241000208340 Araliaceae Species 0.000 title claims abstract description 49
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 title claims abstract description 49
- 235000003140 Panax quinquefolius Nutrition 0.000 title claims abstract description 49
- 235000008434 ginseng Nutrition 0.000 title claims abstract description 49
- 238000000605 extraction Methods 0.000 claims abstract description 112
- 239000003814 drug Substances 0.000 claims abstract description 105
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 48
- 239000007788 liquid Substances 0.000 claims abstract description 48
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 24
- 238000010438 heat treatment Methods 0.000 claims abstract description 22
- 238000003860 storage Methods 0.000 claims abstract description 20
- 238000007789 sealing Methods 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 42
- 238000003756 stirring Methods 0.000 claims description 31
- 238000005406 washing Methods 0.000 claims description 24
- 239000000725 suspension Substances 0.000 claims description 14
- 239000007853 buffer solution Substances 0.000 claims description 12
- 239000003480 eluent Substances 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 238000001514 detection method Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- AXTGDCSMTYGJND-UHFFFAOYSA-N 1-dodecylazepan-2-one Chemical compound CCCCCCCCCCCCN1CCCCCC1=O AXTGDCSMTYGJND-UHFFFAOYSA-N 0.000 claims description 4
- PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 claims description 4
- 230000009089 cytolysis Effects 0.000 claims description 4
- 229910052731 fluorine Inorganic materials 0.000 claims description 4
- 239000011737 fluorine Substances 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- CIDGKBCDYFUZNN-UHFFFAOYSA-N sulfinylmethanone Chemical compound O=C=S=O CIDGKBCDYFUZNN-UHFFFAOYSA-N 0.000 claims description 4
- 238000009736 wetting Methods 0.000 claims description 4
- 230000005389 magnetism Effects 0.000 claims description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 238000010030 laminating Methods 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 description 7
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- XNCSCQSQSGDGES-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]propyl-(carboxymethyl)amino]acetic acid Chemical compound OC(=O)CN(CC(O)=O)C(C)CN(CC(O)=O)CC(O)=O XNCSCQSQSGDGES-UHFFFAOYSA-N 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- VYTBPJNGNGMRFH-UHFFFAOYSA-N acetic acid;azane Chemical compound N.N.CC(O)=O.CC(O)=O.CC(O)=O.CC(O)=O VYTBPJNGNGMRFH-UHFFFAOYSA-N 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 229960000789 guanidine hydrochloride Drugs 0.000 description 2
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 2
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- 241000219470 Mirabilis Species 0.000 description 1
- 240000004371 Panax ginseng Species 0.000 description 1
- 240000007643 Phytolacca americana Species 0.000 description 1
- 235000009074 Phytolacca americana Nutrition 0.000 description 1
- 240000001417 Vigna umbellata Species 0.000 description 1
- 235000011453 Vigna umbellata Nutrition 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
Abstract
The invention discloses a kit for identifying the authenticity of ginseng, which comprises a base, wherein a liquid nitrogen storage tank, a first extraction tank, a vacuumizing device and a second extraction tank are arranged on the base; the first extraction tank is a sealing structure, a cover body of the first extraction tank is provided with a first connecting pipe, a second connecting pipe and a third connecting pipe, and the first connecting pipe, the second connecting pipe and the third connecting pipe are communicated with the first extraction tank; the liquid nitrogen storage tank is communicated with the first connecting pipe, and a first valve body is arranged on a pipeline between the liquid nitrogen storage tank and the first connecting pipe; the second extraction tank is communicated with the second connecting pipe, and a second valve body is arranged on a pipeline between the second extraction tank and the second connecting pipe; the vacuumizing device is communicated with the third connecting pipe, and a third valve body is arranged on a pipeline between the vacuumizing device and the third connecting pipe; be equipped with the heating base in the base, the first bottom of drawing the jar with the laminating of heating base. The medicine box structure is beneficial to rapidly detecting the authenticity of the ginseng.
Description
Technical Field
The invention relates to a kit for identifying the authenticity of ginseng, belonging to the technical field of ginseng authenticity identification.
Background
Ginseng (p.ginseng), called "king of hundred herbs", has the efficacies of reinforcing vital energy, tonifying spleen and lung, promoting the production of body fluid to quench thirst, soothing the nerves and improving intelligence, is the most widely used famous and precious Chinese medicinal material in China, and is distributed in northeast China, far east Russian areas, Korean and other places. In recent years, with the urgent need of people to return to nature and the internationalization of the Chinese herbal medicine market, the utilization of medicinal plants such as ginseng is not limited to the traditional medicine application for treating diseases, but is widely applied to various aspects of human life such as diet, health care, livestock and poultry industry and the like, so that the resource is over-developed and utilized, a considerable part of precious medicinal plant resources are seriously damaged or endangered to be extinct, meanwhile, pseudo-mixed products on the market are on the spot, and the phenomenon of using wild red bean roots, pokeberry roots, mirabilis roots, coggygria roots and the like to supplement ginseng is rare, so that the benefits of consumers are seriously damaged, and an accurate and rapid ginseng detection technology is urgently required to be established.
Disclosure of Invention
The invention aims to provide a kit for identifying the authenticity of ginseng, and the kit structure is beneficial to rapidly detecting the authenticity of ginseng.
In order to solve the technical problems, the invention adopts the following technical scheme:
a kit for identifying ginseng authenticity comprises a base, wherein a liquid nitrogen storage tank, a first extraction tank, a vacuumizing device and a second extraction tank are arranged on the base; the first extraction tank is a sealing structure, a cover body of the first extraction tank is provided with a first connecting pipe, a second connecting pipe and a third connecting pipe, and the first connecting pipe, the second connecting pipe and the third connecting pipe are communicated with the first extraction tank; the liquid nitrogen storage tank is communicated with the first connecting pipe, and a first valve body is arranged on a pipeline between the liquid nitrogen storage tank and the first connecting pipe; the second extraction tank is communicated with the second connecting pipe, and a second valve body is arranged on a pipeline between the second extraction tank and the second connecting pipe; the vacuumizing device is communicated with the third connecting pipe, and a third valve body is arranged on a pipeline between the vacuumizing device and the third connecting pipe; a heating base is arranged in the base, and the bottom of the first extraction tank is attached to the heating base; a driving device is arranged below a first extraction tank in the base, a stirring rod is arranged in the first extraction tank, and the driving device can drive the stirring rod to rotate in the first extraction tank; this kit structure still includes first medicament bottle, second medicament bottle, third medicament bottle, fourth medicament bottle, fifth medicament bottle and sixth medicament bottle, and first medicament bottle, second medicament bottle, third medicament bottle, fourth medicament bottle, fifth medicament bottle and sixth medicament bottle all draw jar intercommunication with the second.
In the kit for identifying the authenticity of the ginseng, the driving device comprises a driving motor and a magnetic strip, the magnetic strip is installed on an output shaft of the driving motor, the stirring rod is magnetic, and the stirring rod is located at the bottom of the first extraction tank and at a position corresponding to the magnetic strip.
In the kit for identifying the authenticity of the ginseng, the heating base is provided with a cylindrical first groove body, and the bottom of the first extraction tank is positioned in the first groove body; the center position of the bottom of the first groove body is provided with a cylindrical second groove body, and the driving device is positioned in the second groove body.
The kit for identifying the authenticity of ginseng comprises the following using methods: sequentially filling a buffer solution, a binding solution, a coarse washing solution, a fine washing solution, an eluent and a suspension into the first medicament bottle, the second medicament bottle, the third medicament bottle, the fourth medicament bottle, the fifth medicament bottle and the sixth medicament bottle; the using method comprises the following steps:
s100: slicing Ginseng radix, placing into a first extraction tank, filling liquid nitrogen in a liquid nitrogen storage tank into the first extraction tank, and then crushing Ginseng radix slices by a driving device and a stirring rod;
s200: adding water-wetting solution and lysis solution into a first extraction tank to soak the ginseng slices, keeping the first extraction tank sealed, and vacuumizing the first extraction tank through a vacuumizing device to ensure that the pressure in the first extraction tank is greater than 0.3 atmospheric pressure and less than 0.5 atmospheric pressure; the vacuum-pumping is performed to reduce the boiling point of the liquid in the first extraction tank and boil the liquid at a lower temperature, so that the absorption of the ginseng to the reagent can be accelerated, and the identification speed can be increased. If no vacuum is applied, the time delay of this stage is over 130%, and if the temperature is too high, the detection accuracy is affected.
S300: heating the first extraction tank by the heating base, raising the temperature of liquid in the first extraction tank to 40-50 ℃, and stirring the liquid in the first extraction tank by the driving device and the stirring rod;
s400: extracting the supernatant of the liquid in the first extraction tank, and introducing the supernatant into the second extraction tank;
s500: introducing buffer solution, binding solution, crude washing solution, refined washing solution, eluent and suspension in a first reagent bottle, a second reagent bottle, a third reagent bottle, a fourth reagent bottle, a fifth reagent bottle and a sixth reagent bottle into a second extraction tank, stirring and standing for 10 minutes;
s600: taking the supernatant in the second extraction tank, and carrying out fluorescence PCR detection.
In the kit for identifying the authenticity of the ginseng, the water-soluble liquid is prepared from the following raw materials in parts by weight: 19 parts of ethanol, 30 parts of azone, 21 parts of thiaketone and 25 parts of organic fluorine.
Compared with the prior art, the medicine box structure is beneficial to rapidly detecting the authenticity of the ginseng. Especially suitable for wild dry ginseng, the artificially planted fresh ginseng has low price, the pseudo-fresh ginseng is very little, and the wild dry ginseng and the artificially planted dry ginseng have obvious difference in shape. The conventional method for detecting the wild dry ginseng needs 18-24 hours, and the structure only needs 3-5 hours, so that the method has extremely high accuracy and consumes very short time.
Drawings
Fig. 1 is a schematic structural diagram of an embodiment of the present invention.
Reference numerals: the device comprises a base 1, a liquid nitrogen storage tank 2, a first valve body 3, a first connecting pipe 4, a second connecting pipe 5, a third connecting pipe 6, a third valve body 7, a second valve body 8, a first extraction tank 9, a vacuum extractor 10, a first medicament bottle 11, a second medicament bottle 12, a third medicament bottle 13, a fourth medicament bottle 14, a fifth medicament bottle 15, a sixth medicament bottle 16, a second extraction tank 17, a heating base 18, a driving device 19, a driving motor 20, a magnetic strip 21 and a stirring rod 22.
The invention is further described with reference to the following figures and detailed description.
Detailed Description
Example 1 of the invention: a kit for identifying ginseng authenticity comprises a base 1, wherein a liquid nitrogen storage tank 2, a first extraction tank 9, a vacuumizing device 10 and a second extraction tank 17 are arranged on the base 1; the first extraction tank 9 is a sealing structure, a cover body of the first extraction tank 9 is provided with a first connecting pipe 4, a second connecting pipe 5 and a third connecting pipe 6, and the first connecting pipe 4, the second connecting pipe 5 and the third connecting pipe 6 are communicated with the first extraction tank 9; the liquid nitrogen storage tank 2 is communicated with the first connecting pipe 4, and a first valve body 3 is arranged on a pipeline between the liquid nitrogen storage tank 2 and the first connecting pipe 4; the second extraction tank 17 is communicated with the second connecting pipe 5, and a second valve body 8 is arranged on a pipeline between the second extraction tank 17 and the second connecting pipe 5; the vacuumizing device 10 is communicated with the third connecting pipe 6, and a third valve body 7 is arranged on a pipeline between the vacuumizing device 10 and the third connecting pipe 6; a heating base 18 is arranged in the base 1, and the bottom of the first extraction tank 9 is attached to the heating base 18; a driving device 19 is arranged below the first extraction tank 9 in the base 1, a stirring rod 22 is arranged in the first extraction tank 9, and the driving device 19 can drive the stirring rod 22 to rotate in the first extraction tank 9; this kit structure still includes first medicament bottle 11, second medicament bottle 12, third medicament bottle 13, fourth medicament bottle 14, fifth medicament bottle 15 and sixth medicament bottle 16, and first medicament bottle 11, second medicament bottle 12, third medicament bottle 13, fourth medicament bottle 14, fifth medicament bottle 15 and sixth medicament bottle 16 all draw jar 17 intercommunication with the second.
The use method of the medicine box structure comprises the following steps: sequentially filling a buffer solution, a binding solution, a crude washing solution, a fine washing solution, an eluent and a suspension into a first medicament bottle 11, a second medicament bottle 12, a third medicament bottle 13, a fourth medicament bottle 14, a fifth medicament bottle 15 and a sixth medicament bottle 16; the using method comprises the following steps:
s100: slicing Ginseng radix and placing into a first extraction tank 9, filling liquid nitrogen in a liquid nitrogen storage tank 2 into the first extraction tank 9, and then crushing Ginseng radix slices by a driving device 19 and a stirring rod 22;
s200: adding water wetting solution and lysis solution into a first extraction tank 9 to soak ginseng slices, keeping the first extraction tank 9 sealed, and vacuumizing the first extraction tank 9 through a vacuumizing device 10 to ensure that the pressure in the first extraction tank 9 is greater than 0.3 atmospheric pressure and less than 0.5 atmospheric pressure; the water-moistening liquid consists of the following raw materials in parts by weight: 19 parts of ethanol, 30 parts of azone, 21 parts of thiaketone and 25 parts of organic fluorine; the water-moistening solution can accelerate the absorption of ginseng to various medicaments, and is favorable for quickly extracting the DNA of the ginseng.
S300: heating the first extraction tank 9 by a heating base 18, raising the temperature of the liquid in the first extraction tank 9 to 40-50 ℃, and stirring the liquid in the first extraction tank 9 by a driving device 19 and a stirring rod 22;
s400: extracting the supernatant of the liquid in the first extraction tank 9 and introducing the supernatant into the second extraction tank 17;
s500: introducing the buffer solution, the binding solution, the crude washing solution, the refined washing solution, the eluent and the suspension in the first medicament bottle 11, the second medicament bottle 12, the third medicament bottle 13, the fourth medicament bottle 14, the fifth medicament bottle 15 and the sixth medicament bottle 16 into a second extraction tank 17, stirring and standing for 10 minutes; the buffer solution, the binding solution, the crude washing solution, the refined washing solution, the eluent and the suspension solution adopt the conventional medicaments. Such as: buffer solution: 11 parts of urea, 6 parts of sodium acetate and 13 parts of beta-mercaptoethanol; binding liquid: guanidine hydrochloride 9, diamine tetraacetic acid 13; crude washing liquid: isopropanol 5, guanidinium isothiocyanate 9; washing liquid: glycine 8 and deionized water 19; eluent: 11m tris-HCl buffer 11, MEDTA 17; the suspension is a suspension of magnetic beads.
S600: the supernatant in the second extraction tank 17 was taken and subjected to fluorescent PCR detection.
Example 2: a kit for identifying ginseng authenticity comprises a base 1, wherein a liquid nitrogen storage tank 2, a first extraction tank 9, a vacuumizing device 10 and a second extraction tank 17 are arranged on the base 1; the first extraction tank 9 is a sealing structure, a cover body of the first extraction tank 9 is provided with a first connecting pipe 4, a second connecting pipe 5 and a third connecting pipe 6, and the first connecting pipe 4, the second connecting pipe 5 and the third connecting pipe 6 are communicated with the first extraction tank 9; the liquid nitrogen storage tank 2 is communicated with the first connecting pipe 4, and a first valve body 3 is arranged on a pipeline between the liquid nitrogen storage tank 2 and the first connecting pipe 4; the second extraction tank 17 is communicated with the second connecting pipe 5, and a second valve body 8 is arranged on a pipeline between the second extraction tank 17 and the second connecting pipe 5; the vacuumizing device 10 is communicated with the third connecting pipe 6, and a third valve body 7 is arranged on a pipeline between the vacuumizing device 10 and the third connecting pipe 6; a heating base 18 is arranged in the base 1, and the bottom of the first extraction tank 9 is attached to the heating base 18; a driving device 19 is arranged below the first extraction tank 9 in the base 1, a stirring rod 22 is arranged in the first extraction tank 9, and the driving device 19 can drive the stirring rod 22 to rotate in the first extraction tank 9; this kit structure still includes first medicament bottle 11, second medicament bottle 12, third medicament bottle 13, fourth medicament bottle 14, fifth medicament bottle 15 and sixth medicament bottle 16, and first medicament bottle 11, second medicament bottle 12, third medicament bottle 13, fourth medicament bottle 14, fifth medicament bottle 15 and sixth medicament bottle 16 all draw jar 17 intercommunication with the second.
The driving device 19 comprises a driving motor 20 and a magnetic strip 21, the magnetic strip 21 is installed on an output shaft of the driving motor 20, the stirring rod 22 has magnetism, and the stirring rod 22 is located at the bottom of the first extraction tank 9 and at a position corresponding to the magnetic strip 21. The heating base 18 is provided with a cylindrical first groove body, and the bottom of the first extraction tank 9 is positioned in the first groove body; the center position of the bottom of the first groove body is provided with a cylindrical second groove body, and the driving device 19 is positioned in the second groove body.
The use method of the medicine box structure comprises the following steps: sequentially filling a buffer solution, a binding solution, a crude washing solution, a fine washing solution, an eluent and a suspension into a first medicament bottle 11, a second medicament bottle 12, a third medicament bottle 13, a fourth medicament bottle 14, a fifth medicament bottle 15 and a sixth medicament bottle 16; the using method comprises the following steps:
s100: slicing Ginseng radix and placing into a first extraction tank 9, filling liquid nitrogen in a liquid nitrogen storage tank 2 into the first extraction tank 9, and then crushing Ginseng radix slices by a driving device 19 and a stirring rod 22;
s200: adding water wetting solution and lysis solution into a first extraction tank 9 to soak ginseng slices, keeping the first extraction tank 9 sealed, and vacuumizing the first extraction tank 9 through a vacuumizing device 10 to ensure that the pressure in the first extraction tank 9 is greater than 0.3 atmospheric pressure and less than 0.5 atmospheric pressure; the water-moistening liquid consists of the following raw materials in parts by weight: 19 parts of ethanol, 30 parts of azone, 21 parts of thiaketone and 25 parts of organic fluorine; the water-moistening solution can accelerate the absorption of ginseng to various medicaments, and is favorable for quickly extracting the DNA of the ginseng.
S300: heating the first extraction tank 9 by a heating base 18, raising the temperature of the liquid in the first extraction tank 9 to 40-50 ℃, and stirring the liquid in the first extraction tank 9 by a driving device 19 and a stirring rod 22;
s400: extracting the supernatant of the liquid in the first extraction tank 9 and introducing the supernatant into the second extraction tank 17;
s500: introducing the buffer solution, the binding solution, the crude washing solution, the refined washing solution, the eluent and the suspension in the first medicament bottle 11, the second medicament bottle 12, the third medicament bottle 13, the fourth medicament bottle 14, the fifth medicament bottle 15 and the sixth medicament bottle 16 into a second extraction tank 17, stirring and standing for 10 minutes; the buffer solution, the binding solution, the crude washing solution, the refined washing solution, the eluent and the suspension solution adopt the conventional medicaments. Such as: buffer solution: 11 parts of urea, 6 parts of sodium acetate and 13 parts of beta-mercaptoethanol; binding liquid: guanidine hydrochloride 9, diamine tetraacetic acid 13; crude washing liquid: isopropanol 5, guanidinium isothiocyanate 9; washing liquid: glycine 8 and deionized water 19; eluent: 11m tris-HCl buffer 11, MEDTA 17; the suspension is a suspension of magnetic beads.
S600: the supernatant in the second extraction tank 17 was taken and subjected to fluorescent PCR detection.
Claims (5)
1. A kit for identifying the authenticity of ginseng is characterized by comprising a base (1), wherein a liquid nitrogen storage tank (2), a first extraction tank (9), a vacuumizing device (10) and a second extraction tank (17) are arranged on the base (1); the first extraction tank (9) is of a sealing structure, a cover body of the first extraction tank (9) is provided with a first connecting pipe (4), a second connecting pipe (5) and a third connecting pipe (6), and the first connecting pipe (4), the second connecting pipe (5) and the third connecting pipe (6) are communicated with the first extraction tank (9); the liquid nitrogen storage tank (2) is communicated with the first connecting pipe (4), and a first valve body (3) is arranged on a pipeline between the liquid nitrogen storage tank (2) and the first connecting pipe (4); the second extraction tank (17) is communicated with the second connecting pipe (5), and a second valve body (8) is arranged on a pipeline between the second extraction tank (17) and the second connecting pipe (5); the vacuumizing device (10) is communicated with the third connecting pipe (6), and a third valve body (7) is arranged on a pipeline between the vacuumizing device (10) and the third connecting pipe (6); a heating base (18) is arranged in the base (1), and the bottom of the first extraction tank (9) is attached to the heating base (18); a driving device (19) is arranged below a first extraction tank (9) in the base (1), a stirring rod (22) is arranged in the first extraction tank (9), and the driving device (19) can drive the stirring rod (22) to rotate in the first extraction tank (9); this kit structure still includes first medicament bottle (11), second medicament bottle (12), third medicament bottle (13), fourth medicament bottle (14), fifth medicament bottle (15) and sixth medicament bottle (16), and first medicament bottle (11), second medicament bottle (12), third medicament bottle (13), fourth medicament bottle (14), fifth medicament bottle (15) and sixth medicament bottle (16) all draw jar (17) intercommunication with the second.
2. The kit for authenticating ginseng according to claim 1, wherein the driving means (19) comprises a driving motor (20) and a magnetic strip (21), the magnetic strip (21) is mounted on an output shaft of the driving motor (20), the stirring rod (22) has magnetism, and the stirring rod (22) is positioned at a position corresponding to the magnetic strip (21) at the bottom of the first extraction pot (9).
3. The kit for verifying ginseng authenticity according to claim 2, wherein the heating base (18) has a first groove body having a cylindrical shape, and the bottom of the first extraction tank (9) is located in the first groove body; the center position of the bottom of the first groove body is provided with a cylindrical second groove body, and the driving device (19) is positioned in the second groove body.
4. The kit for verifying the authenticity of ginseng according to claim 3, wherein the following methods are used: sequentially filling a buffer solution, a binding solution, a coarse washing solution, a fine washing solution, an eluent and a suspension into a first medicament bottle (11), a second medicament bottle (12), a third medicament bottle (13), a fourth medicament bottle (14), a fifth medicament bottle (15) and a sixth medicament bottle (16); the using method comprises the following steps:
s100: slicing ginseng and placing the sliced ginseng into a first extraction tank (9), filling liquid nitrogen in a liquid nitrogen storage tank (2) into the first extraction tank (9), and then smashing the sliced ginseng through a driving device (19) and a stirring rod (22);
s200: adding a water wetting solution and a lysis solution into a first extraction tank (9) to soak ginseng slices, keeping the first extraction tank (9) sealed, and vacuumizing the first extraction tank (9) through a vacuumizing device (10) to ensure that the pressure in the first extraction tank (9) is greater than 0.3 atmospheric pressure and less than 0.5 atmospheric pressure;
s300: heating the first extraction tank (9) by a heating base (18), raising the temperature of liquid in the first extraction tank (9) to 40-50 ℃, and stirring the liquid in the first extraction tank (9) by a driving device (19) and a stirring rod (22);
s400: extracting the supernatant of the liquid in the first extraction tank (9) and introducing the supernatant into the second extraction tank (17);
s500: introducing buffer solution, binding solution, crude washing solution, refined washing solution, eluent and suspension in a first medicament bottle (11), a second medicament bottle (12), a third medicament bottle (13), a fourth medicament bottle (14), a fifth medicament bottle (15) and a sixth medicament bottle (16) into a second extraction tank (17), stirring and standing for 10 minutes;
s600: taking the supernatant in the second extraction tank (17), and carrying out fluorescence PCR detection.
5. The kit for identifying the authenticity of the ginseng according to claim 4, wherein the water-based moistening liquid is prepared from the following raw materials in parts by weight: 19 parts of ethanol, 30 parts of azone, 21 parts of thiaketone and 25 parts of organic fluorine.
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