CN112514890A - Normal-temperature short-term preservation diluent for fresh sheep essence and preparation method thereof - Google Patents
Normal-temperature short-term preservation diluent for fresh sheep essence and preparation method thereof Download PDFInfo
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- CN112514890A CN112514890A CN202011506138.0A CN202011506138A CN112514890A CN 112514890 A CN112514890 A CN 112514890A CN 202011506138 A CN202011506138 A CN 202011506138A CN 112514890 A CN112514890 A CN 112514890A
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- 239000003085 diluting agent Substances 0.000 title claims abstract description 55
- 241001494479 Pecora Species 0.000 title claims abstract description 54
- 238000004321 preservation Methods 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 210000000582 semen Anatomy 0.000 claims abstract description 72
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 33
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 23
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 22
- 239000012154 double-distilled water Substances 0.000 claims abstract description 16
- 150000001875 compounds Chemical class 0.000 claims abstract description 14
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000002156 mixing Methods 0.000 claims abstract description 12
- 239000001509 sodium citrate Substances 0.000 claims abstract description 12
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 12
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229930091371 Fructose Natural products 0.000 claims abstract description 11
- 239000005715 Fructose Substances 0.000 claims abstract description 11
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims abstract description 11
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 11
- 229930003270 Vitamin B Natural products 0.000 claims abstract description 11
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 11
- 239000012895 dilution Substances 0.000 claims abstract description 11
- 238000010790 dilution Methods 0.000 claims abstract description 11
- 239000008103 glucose Substances 0.000 claims abstract description 11
- 239000011780 sodium chloride Substances 0.000 claims abstract description 11
- 235000019156 vitamin B Nutrition 0.000 claims abstract description 11
- 239000011720 vitamin B Substances 0.000 claims abstract description 11
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 11
- 239000011718 vitamin C Substances 0.000 claims abstract description 11
- 239000007983 Tris buffer Substances 0.000 claims abstract 3
- 230000003115 biocidal effect Effects 0.000 claims description 15
- 239000000706 filtrate Substances 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 10
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical group CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 9
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- 239000000047 product Substances 0.000 claims description 5
- 238000012216 screening Methods 0.000 claims description 5
- 235000011083 sodium citrates Nutrition 0.000 claims description 5
- 229960005322 streptomycin Drugs 0.000 claims description 5
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 2
- 230000035899 viability Effects 0.000 claims 6
- 241000283707 Capra Species 0.000 claims 1
- 229930182555 Penicillin Natural products 0.000 claims 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical group N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims 1
- 238000011156 evaluation Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 229940049954 penicillin Drugs 0.000 claims 1
- 230000009027 insemination Effects 0.000 abstract description 10
- 238000000338 in vitro Methods 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000004720 fertilization Effects 0.000 abstract 2
- 239000003242 anti bacterial agent Substances 0.000 abstract 1
- 229940088710 antibiotic agent Drugs 0.000 abstract 1
- 230000004899 motility Effects 0.000 description 20
- 238000009395 breeding Methods 0.000 description 10
- 230000001488 breeding effect Effects 0.000 description 10
- 229960001484 edetic acid Drugs 0.000 description 9
- FYFFGSSZFBZTAH-UHFFFAOYSA-N methylaminomethanetriol Chemical compound CNC(O)(O)O FYFFGSSZFBZTAH-UHFFFAOYSA-N 0.000 description 7
- 239000000243 solution Substances 0.000 description 5
- 238000003860 storage Methods 0.000 description 5
- 238000007865 diluting Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 239000011259 mixed solution Substances 0.000 description 4
- 238000013441 quality evaluation Methods 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 230000007547 defect Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
- A01N1/124—Disinfecting agents, e.g. antimicrobials
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明提供了一种羊鲜精常温短期保存稀释液,由以下质量百分比组份构成：三羟甲基氨基甲烷11%—24%，葡萄糖13%—31%、柠檬酸5%—12.3%，果糖3%—7.3%，柠檬酸钠3%—6.5%，维生素C 2.5%—6.1%，维生素B 2.2%—5.5%，EDTA1.2%—2.2%，氯化钠1.9%—2.2%，复合抗生素0.5%—2.8%，余量为双蒸水。其制备方法包括初步混合及调制作业等两个步骤。本发明所涉及的羊精液稀释液及其制备方法，生产成本低，一方面可有效提高种公羊利用效率，增加良种覆盖率，减少人工授精人员配置，提高劳动效率。同时有效提高人工授精受胎率及体外授精效率，便于大规模推广应用。
The invention provides a short-term preservation dilution of sheep fresh essence at room temperature, which is composed of the following components in mass percentage: 11%-24% of tris, 13%-31% of glucose, 5%-12.3% of citric acid, Fructose 3%—7.3%, sodium citrate 3%—6.5%, vitamin C 2.5%—6.1%, vitamin B 2.2%—5.5%, EDTA 1.2%—2.2%, sodium chloride 1.9%—2.2%, compound Antibiotics 0.5%-2.8%, the balance is double distilled water. The preparation method includes two steps: preliminary mixing and preparation. The sheep semen diluent and the preparation method thereof of the present invention have low production cost, on the one hand, can effectively improve the utilization efficiency of rams, increase the coverage rate of good breeds, reduce the allocation of artificial insemination personnel, and improve labor efficiency. At the same time, it can effectively improve the fertilization rate of artificial insemination and the efficiency of in vitro fertilization, which is convenient for large-scale promotion and application.
Description
Technical Field
The invention relates to a normal-temperature short-term preservation diluent of sheep fresh essence and a preparation method thereof, belonging to the technical field of cultivation and artificial propagation.
Background
With the rapid development of the large-scale sheep farm, the breeding technology of sheep gradually becomes a bottleneck for restricting the development and promotion of the sheep farm. When the frozen semen artificial insemination technology is not mature at present, the breeding and fresh semen artificial insemination are still the main breeding mode of the sheep farm. At present, most sheep farms adopt breeding sheep semen of the factory, and the mode used by the breeding sheep of the factory expands the utilization efficiency and the improved variety coverage rate of the breeding sheep. However, because the development of the technical field of preservation of fresh semen is lagged, the traditional diluent is usually prepared and diluted temporarily by adopting 0.9% normal saline, 2.9% sodium citrate or defatted fresh milk and the like, the traditional diluent has the defects of short preservation time, low dilution multiple and serious influence on the utilization efficiency and the improved breed coverage rate of the breeding sheep, thereby causing the problems that both sides of semen collection and hybridization consume a large amount of manpower and material resources, the weight balancing cost is high and the utilization efficiency of the breeding sheep is low.
Therefore, aiming at the problem, a brand-new dilution of the breeding sheep semen is urgently needed to be developed so as to meet the needs of actual breeding work.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a normal-temperature short-term preservation diluent of sheep fresh essence and a preparation method thereof, so as to meet the requirement of practical use.
In order to achieve the purpose, the invention provides the following technical scheme:
a normal-temperature short-term preservation diluent for sheep fresh essence comprises the following components in percentage by mass: 11% -24% of tris (hydroxymethyl) aminomethane, 13% -31% of glucose, 5% -12.3% of citric acid, 3% -7.3% of fructose, 3% -6.5% of sodium citrate, 2.5% -6.1% of vitamin C, 2.2% -5.5% of vitamin B, 1.2% -2.2% of EDTA, 1.9% -2.2% of sodium chloride, 0.5% -2.8% of compound antibiotic and the balance of double distilled water.
Further, the compound antibiotic is 3000-5000 units of streptomycin.
A preparation method of a normal-temperature short-term preservation diluent of sheep fresh essence comprises the following steps:
s1, primarily mixing, namely heating double distilled water to 20-50 ℃ at a constant speed, adding trihydroxymethyl aminomethane, glucose, citric acid, fructose, sodium citrate, vitamin C, vitamin B, EDTA and sodium chloride into the double distilled water, uniformly stirring, filtering the mixed solution through a 0.2-micron filter membrane, collecting the filtrate, and preserving the filtrate at a constant temperature of 20-50 ℃;
s2, preparing, namely adding the compound antibiotic into the filtrate prepared in the step S1, stirring uniformly at constant temperature to obtain a finished product diluent, and then adjusting the temperature of the diluent to 30-38.5 ℃ and storing in a dark place.
Further, the diluent prepared in the step S2 is preserved for 5 to 24 hours.
A use method of a normal-temperature short-term preservation diluent for fresh sheep semen comprises the following steps:
firstly, screening sheep semen, namely firstly, adjusting the temperature of collected fresh ram semen to 37 ℃ for storage, then sampling the fresh ram semen and carrying out quality evaluation on the apparent motility rate, the motility rate and the density of the fresh ram semen through a microscope, wherein the apparent motility rate is set into a quality grade from 0 to 5 according to the fluctuation intensity, then the ram semen with the motility rate of 4 grades, the motility rate of more than 0.7 and the density of more than 20 hundred million/mL is selected as the semen to be diluted to be qualified;
and secondly, diluting, namely adding a diluent into the fresh ram semen in the preservation state selected in the first step, wherein the mixing ratio of the fresh ram semen to the diluent is 1: 5-10, and then transferring the mixed and diluted sheep semen to a semen collection cup for preserving heat for later use.
Furthermore, when the apparent activity rate is divided into quality grades according to the fluctuation intensity, the grade 0 is no fluctuation, and the grade 5 is rapid vortex-shaped fluctuation.
Further, the preservation time of the diluted sheep semen is 14 hours.
The sheep semen diluent and the preparation method thereof have low production cost, and on one hand, the sheep semen diluent can effectively improve the utilization efficiency of stud rams, increase the coverage rate of fine breeds, reduce the configuration of artificial insemination personnel and improve the labor efficiency. Meanwhile, the conception rate of artificial insemination and the efficiency of in vitro insemination are effectively improved, and the method is convenient for large-scale popularization and application.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of the preparation method of the present invention;
FIG. 2 is a flow chart of a method of using the diluent of the present invention;
FIG. 3 is a table comparing the preservation time and dilution ratio of the present invention with the conventional diluent;
figure 4 shows the number of ewes that can be dosed with the diluent of the present invention.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
As shown in fig. 1-4, a normal-temperature short-term preservation diluent of sheep fresh essence comprises the following components in percentage by mass: 11% of trihydroxymethyl aminomethane, 13% of glucose, 5% of citric acid, 3% of fructose, 3% of sodium citrate, 2.5% of vitamin C, 2.2% of vitamin B, 1.2% of EDTA (ethylene diamine tetraacetic acid), 1.9% of sodium chloride, 0.5% of compound antibiotic and the balance of double distilled water.
In this example, the antibiotic cocktail was 3000 units of streptomycin.
A preparation method of a normal-temperature short-term preservation diluent of sheep fresh essence comprises the following steps:
s1, primarily mixing, namely heating double distilled water to 20 ℃ at a constant speed, adding trihydroxymethyl aminomethane, glucose, citric acid, fructose, sodium citrate, vitamin C, vitamin B, EDTA and sodium chloride into the double distilled water, uniformly stirring, filtering the mixed solution through a 0.2 um filter membrane, collecting the filtrate, and preserving the filtrate at a constant temperature of 20 ℃;
s2, preparing, namely adding the compound antibiotic into the filtrate prepared in the step S1, stirring uniformly at constant temperature to obtain a finished product diluent, and then adjusting the temperature of the diluent to 30 ℃ and storing in a dark place.
Meanwhile, the time for storing the diluent prepared in the step S2 is 5 hours.
A use method of a normal-temperature short-term preservation diluent for fresh sheep semen comprises the following steps:
firstly, screening sheep semen, namely firstly, adjusting the temperature of collected fresh ram semen to 37 ℃ for storage, then sampling the fresh ram semen and carrying out quality evaluation on the apparent motility rate, the motility rate and the density of the fresh ram semen through a microscope, wherein the apparent motility rate is set into a quality grade from 0 to 5 according to the fluctuation intensity, then the ram semen with the motility rate of 4 grades, the motility rate of more than 0.7 and the density of more than 20 hundred million/mL is selected as the semen to be diluted to be qualified;
and secondly, diluting, namely adding a diluent into the fresh ram semen in the preservation state selected in the first step, wherein the mixing ratio of the fresh ram semen to the diluent is 1:5, and then transferring the mixed and diluted ram semen into a semen collection cup for preserving heat for later use.
It should be noted that when the apparent activity is classified into quality grades according to the fluctuation intensity, the grade 0 is no fluctuation, and the grade 5 is rapid vortex-like fluctuation.
In addition, the diluted sheep semen solution is preserved for 1 hour.
Example 2
As shown in fig. 1-4, a normal-temperature short-term preservation diluent of sheep fresh essence comprises the following components in percentage by mass: 24% of trihydroxymethyl aminomethane, 31% of glucose, 12.3% of citric acid, 7.3% of fructose, 6.5% of sodium citrate, 6.1% of vitamin C, 5.5% of vitamin B, 2.2% of EDTA, 2.2% of sodium chloride, 2.8% of compound antibiotic and the balance of double distilled water.
Further optimized, the compound antibiotic is 3000-5000 units of streptomycin.
A preparation method of a normal-temperature short-term preservation diluent of sheep fresh essence comprises the following steps:
s1, primarily mixing, namely heating double distilled water to 50 ℃ at a constant speed, adding trihydroxymethyl aminomethane, glucose, citric acid, fructose, sodium citrate, vitamin C, vitamin B, EDTA and sodium chloride into the double distilled water, uniformly stirring, filtering the mixed solution through a 0.2 um filter membrane, collecting the filtrate, and preserving at a constant temperature of 50 ℃;
s2, preparing, namely adding the compound antibiotic into the filtrate prepared in the step S1, stirring uniformly at constant temperature to obtain a finished product diluent, and then adjusting the temperature of the diluent to 38.5 ℃ and storing in dark place.
It should be noted that the diluent prepared in step S2 has a storage time of 24 hours.
A use method of a normal-temperature short-term preservation diluent for fresh sheep semen comprises the following steps:
firstly, screening sheep semen, namely firstly, adjusting the temperature of collected fresh ram semen to 37 ℃ for storage, then sampling the fresh ram semen and carrying out quality evaluation on the apparent motility rate, the motility rate and the density of the fresh ram semen through a microscope, wherein the apparent motility rate is set into a quality grade from 0 to 5 according to the fluctuation intensity, then the ram semen with the motility rate of 4 grades, the motility rate of more than 0.7 and the density of more than 20 hundred million/mL is selected as the semen to be diluted to be qualified;
and secondly, diluting, namely adding a diluent into the fresh ram semen in the preservation state selected in the first step, wherein the mixing ratio of the fresh ram semen to the diluent is 1: 10, and then transferring the mixed and diluted ram semen into a semen collection cup for preserving heat for later use.
It is important to note that when the apparent activity is classified into quality grades according to the fluctuation intensity, the 0 grade is no fluctuation, and the 5 grade is rapid vortex-like fluctuation.
In addition, the diluted sheep semen solution is preserved for 24 hours.
Example 3
As shown in fig. 1-4, a normal-temperature short-term preservation diluent of sheep fresh essence comprises the following components in percentage by mass: 18% of trihydroxymethyl aminomethane, 25% of glucose, 9.5% of citric acid, 4.3% of fructose, 4.8% of sodium citrate, 5.5% of vitamin C, 3.8% of vitamin B, 1.8% of EDTA, 2.0% of sodium chloride, 1.7% of compound antibiotic and the balance of double distilled water.
In this example, the antibiotic complex is 4000 units of streptomycin.
A preparation method of a normal-temperature short-term preservation diluent of sheep fresh essence comprises the following steps:
s1, primarily mixing, namely heating double distilled water to 30 ℃ at a constant speed, adding trihydroxymethyl aminomethane, glucose, citric acid, fructose, sodium citrate, vitamin C, vitamin B, EDTA and sodium chloride into the double distilled water, uniformly stirring, filtering the mixed solution through a 0.2 um filter membrane, collecting the filtrate, and preserving the filtrate at a constant temperature of 40 ℃;
s2, preparing, namely adding the compound antibiotic into the filtrate prepared in the step S1, stirring uniformly at constant temperature to obtain a finished product diluent, and then adjusting the temperature of the diluent to 37 ℃ and storing in a dark place.
In this example, the time for which the diluent prepared in step S2 was stored was 12 hours.
A use method of a normal-temperature short-term preservation diluent for fresh sheep semen comprises the following steps:
firstly, screening sheep semen, namely firstly, adjusting the temperature of collected fresh ram semen to 37 ℃ for storage, then sampling the fresh ram semen and carrying out quality evaluation on the apparent motility rate, the motility rate and the density of the fresh ram semen through a microscope, wherein the apparent motility rate is set into a quality grade from 0 to 5 according to the fluctuation intensity, then the ram semen with the motility rate of 4 grades, the motility rate of more than 0.7 and the density of more than 20 hundred million/mL is selected as the semen to be diluted to be qualified;
and secondly, diluting, namely adding a diluent into the fresh ram semen in the preservation state selected in the first step, wherein the mixing ratio of the fresh ram semen to the diluent is 1:6, and then transferring the mixed and diluted ram semen into a semen collection cup for preserving heat for later use.
Meanwhile, when the apparent activity rate is divided into quality grades according to the fluctuation intensity, the grade 0 is no fluctuation, and the grade 5 is rapid vortex-shaped fluctuation.
In addition, the diluted sheep semen solution is preserved for 14 hours.
The sheep semen diluent and the preparation method thereof have low production cost, and on one hand, the sheep semen diluent can effectively improve the utilization efficiency of stud rams, increase the coverage rate of fine breeds, reduce the configuration of artificial insemination personnel and improve the labor efficiency. Meanwhile, the conception rate of artificial insemination and the efficiency of in vitro insemination are effectively improved, and the method is convenient for large-scale popularization and application.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (7)
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3973003A (en) * | 1973-12-18 | 1976-08-03 | Institut National De La Recherche Agronomique | Process for the preservation of ram semen by freezing |
| CN103518706A (en) * | 2013-10-21 | 2014-01-22 | 西北农林科技大学 | Diluent of frozen semen of milk goat, and preparation method and diluting method thereof as well as preparation method of frozen semen of milk goat in thin pipe |
| CN103548812A (en) * | 2013-10-30 | 2014-02-05 | 西北农林科技大学 | Normal-temperature and low-temperature preservation diluents of milk goat seminal fluid |
| CN104068013A (en) * | 2014-07-17 | 2014-10-01 | 赤峰市农牧科学研究院 | Sheep semen dilution preserving fluid and preparation method thereof |
| CN109006802A (en) * | 2018-07-08 | 2018-12-18 | 乌鲁木齐九品芝麻信息科技有限公司 | Store method when a kind of fresh essence of sheep is long |
-
2020
- 2020-12-18 CN CN202011506138.0A patent/CN112514890A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3973003A (en) * | 1973-12-18 | 1976-08-03 | Institut National De La Recherche Agronomique | Process for the preservation of ram semen by freezing |
| CN103518706A (en) * | 2013-10-21 | 2014-01-22 | 西北农林科技大学 | Diluent of frozen semen of milk goat, and preparation method and diluting method thereof as well as preparation method of frozen semen of milk goat in thin pipe |
| CN103548812A (en) * | 2013-10-30 | 2014-02-05 | 西北农林科技大学 | Normal-temperature and low-temperature preservation diluents of milk goat seminal fluid |
| CN104068013A (en) * | 2014-07-17 | 2014-10-01 | 赤峰市农牧科学研究院 | Sheep semen dilution preserving fluid and preparation method thereof |
| CN109006802A (en) * | 2018-07-08 | 2018-12-18 | 乌鲁木齐九品芝麻信息科技有限公司 | Store method when a kind of fresh essence of sheep is long |
Non-Patent Citations (1)
| Title |
|---|
| 朱兴贵等主编: "《畜禽繁育技术》", 31 July 2015, 中国轻工业出版社 * |
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Application publication date: 20210319 |