CN112514890A - Normal-temperature short-term preservation diluent for fresh sheep essence and preparation method thereof - Google Patents
Normal-temperature short-term preservation diluent for fresh sheep essence and preparation method thereof Download PDFInfo
- Publication number
- CN112514890A CN112514890A CN202011506138.0A CN202011506138A CN112514890A CN 112514890 A CN112514890 A CN 112514890A CN 202011506138 A CN202011506138 A CN 202011506138A CN 112514890 A CN112514890 A CN 112514890A
- Authority
- CN
- China
- Prior art keywords
- diluent
- sheep
- semen
- fresh
- normal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0215—Disinfecting agents, e.g. antimicrobials for preserving living parts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a normal-temperature short-term preservation diluent for sheep fresh essence, which comprises the following components in percentage by mass: 11% -24% of tris (hydroxymethyl) aminomethane, 13% -31% of glucose, 5% -12.3% of citric acid, 3% -7.3% of fructose, 3% -6.5% of sodium citrate, 2.5% -6.1% of vitamin C, 2.2% -5.5% of vitamin B, 1.2% -2.2% of EDTA, 1.9% -2.2% of sodium chloride, 0.5% -2.8% of compound antibiotic and the balance of double distilled water. The preparation method comprises two steps of preliminary mixing, modulation operation and the like. The sheep semen diluent and the preparation method thereof have low production cost, and on one hand, the sheep semen diluent can effectively improve the utilization efficiency of stud rams, increase the coverage rate of fine breeds, reduce the configuration of artificial insemination personnel and improve the labor efficiency. Meanwhile, the conception rate of artificial insemination and the efficiency of in vitro insemination are effectively improved, and the method is convenient for large-scale popularization and application.
Description
Technical Field
The invention relates to a normal-temperature short-term preservation diluent of sheep fresh essence and a preparation method thereof, belonging to the technical field of cultivation and artificial propagation.
Background
With the rapid development of the large-scale sheep farm, the breeding technology of sheep gradually becomes a bottleneck for restricting the development and promotion of the sheep farm. When the frozen semen artificial insemination technology is not mature at present, the breeding and fresh semen artificial insemination are still the main breeding mode of the sheep farm. At present, most sheep farms adopt breeding sheep semen of the factory, and the mode used by the breeding sheep of the factory expands the utilization efficiency and the improved variety coverage rate of the breeding sheep. However, because the development of the technical field of preservation of fresh semen is lagged, the traditional diluent is usually prepared and diluted temporarily by adopting 0.9% normal saline, 2.9% sodium citrate or defatted fresh milk and the like, the traditional diluent has the defects of short preservation time, low dilution multiple and serious influence on the utilization efficiency and the improved breed coverage rate of the breeding sheep, thereby causing the problems that both sides of semen collection and hybridization consume a large amount of manpower and material resources, the weight balancing cost is high and the utilization efficiency of the breeding sheep is low.
Therefore, aiming at the problem, a brand-new dilution of the breeding sheep semen is urgently needed to be developed so as to meet the needs of actual breeding work.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a normal-temperature short-term preservation diluent of sheep fresh essence and a preparation method thereof, so as to meet the requirement of practical use.
In order to achieve the purpose, the invention provides the following technical scheme:
a normal-temperature short-term preservation diluent for sheep fresh essence comprises the following components in percentage by mass: 11% -24% of tris (hydroxymethyl) aminomethane, 13% -31% of glucose, 5% -12.3% of citric acid, 3% -7.3% of fructose, 3% -6.5% of sodium citrate, 2.5% -6.1% of vitamin C, 2.2% -5.5% of vitamin B, 1.2% -2.2% of EDTA, 1.9% -2.2% of sodium chloride, 0.5% -2.8% of compound antibiotic and the balance of double distilled water.
Further, the compound antibiotic is 3000-5000 units of streptomycin.
A preparation method of a normal-temperature short-term preservation diluent of sheep fresh essence comprises the following steps:
s1, primarily mixing, namely heating double distilled water to 20-50 ℃ at a constant speed, adding trihydroxymethyl aminomethane, glucose, citric acid, fructose, sodium citrate, vitamin C, vitamin B, EDTA and sodium chloride into the double distilled water, uniformly stirring, filtering the mixed solution through a 0.2-micron filter membrane, collecting the filtrate, and preserving the filtrate at a constant temperature of 20-50 ℃;
s2, preparing, namely adding the compound antibiotic into the filtrate prepared in the step S1, stirring uniformly at constant temperature to obtain a finished product diluent, and then adjusting the temperature of the diluent to 30-38.5 ℃ and storing in a dark place.
Further, the diluent prepared in the step S2 is preserved for 5 to 24 hours.
A use method of a normal-temperature short-term preservation diluent for fresh sheep semen comprises the following steps:
firstly, screening sheep semen, namely firstly, adjusting the temperature of collected fresh ram semen to 37 ℃ for storage, then sampling the fresh ram semen and carrying out quality evaluation on the apparent motility rate, the motility rate and the density of the fresh ram semen through a microscope, wherein the apparent motility rate is set into a quality grade from 0 to 5 according to the fluctuation intensity, then the ram semen with the motility rate of 4 grades, the motility rate of more than 0.7 and the density of more than 20 hundred million/mL is selected as the semen to be diluted to be qualified;
and secondly, diluting, namely adding a diluent into the fresh ram semen in the preservation state selected in the first step, wherein the mixing ratio of the fresh ram semen to the diluent is 1: 5-10, and then transferring the mixed and diluted sheep semen to a semen collection cup for preserving heat for later use.
Furthermore, when the apparent activity rate is divided into quality grades according to the fluctuation intensity, the grade 0 is no fluctuation, and the grade 5 is rapid vortex-shaped fluctuation.
Further, the preservation time of the diluted sheep semen is 14 hours.
The sheep semen diluent and the preparation method thereof have low production cost, and on one hand, the sheep semen diluent can effectively improve the utilization efficiency of stud rams, increase the coverage rate of fine breeds, reduce the configuration of artificial insemination personnel and improve the labor efficiency. Meanwhile, the conception rate of artificial insemination and the efficiency of in vitro insemination are effectively improved, and the method is convenient for large-scale popularization and application.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of the preparation method of the present invention;
FIG. 2 is a flow chart of a method of using the diluent of the present invention;
FIG. 3 is a table comparing the preservation time and dilution ratio of the present invention with the conventional diluent;
figure 4 shows the number of ewes that can be dosed with the diluent of the present invention.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
As shown in fig. 1-4, a normal-temperature short-term preservation diluent of sheep fresh essence comprises the following components in percentage by mass: 11% of trihydroxymethyl aminomethane, 13% of glucose, 5% of citric acid, 3% of fructose, 3% of sodium citrate, 2.5% of vitamin C, 2.2% of vitamin B, 1.2% of EDTA (ethylene diamine tetraacetic acid), 1.9% of sodium chloride, 0.5% of compound antibiotic and the balance of double distilled water.
In this example, the antibiotic cocktail was 3000 units of streptomycin.
A preparation method of a normal-temperature short-term preservation diluent of sheep fresh essence comprises the following steps:
s1, primarily mixing, namely heating double distilled water to 20 ℃ at a constant speed, adding trihydroxymethyl aminomethane, glucose, citric acid, fructose, sodium citrate, vitamin C, vitamin B, EDTA and sodium chloride into the double distilled water, uniformly stirring, filtering the mixed solution through a 0.2 um filter membrane, collecting the filtrate, and preserving the filtrate at a constant temperature of 20 ℃;
s2, preparing, namely adding the compound antibiotic into the filtrate prepared in the step S1, stirring uniformly at constant temperature to obtain a finished product diluent, and then adjusting the temperature of the diluent to 30 ℃ and storing in a dark place.
Meanwhile, the time for storing the diluent prepared in the step S2 is 5 hours.
A use method of a normal-temperature short-term preservation diluent for fresh sheep semen comprises the following steps:
firstly, screening sheep semen, namely firstly, adjusting the temperature of collected fresh ram semen to 37 ℃ for storage, then sampling the fresh ram semen and carrying out quality evaluation on the apparent motility rate, the motility rate and the density of the fresh ram semen through a microscope, wherein the apparent motility rate is set into a quality grade from 0 to 5 according to the fluctuation intensity, then the ram semen with the motility rate of 4 grades, the motility rate of more than 0.7 and the density of more than 20 hundred million/mL is selected as the semen to be diluted to be qualified;
and secondly, diluting, namely adding a diluent into the fresh ram semen in the preservation state selected in the first step, wherein the mixing ratio of the fresh ram semen to the diluent is 1:5, and then transferring the mixed and diluted ram semen into a semen collection cup for preserving heat for later use.
It should be noted that when the apparent activity is classified into quality grades according to the fluctuation intensity, the grade 0 is no fluctuation, and the grade 5 is rapid vortex-like fluctuation.
In addition, the diluted sheep semen solution is preserved for 1 hour.
Example 2
As shown in fig. 1-4, a normal-temperature short-term preservation diluent of sheep fresh essence comprises the following components in percentage by mass: 24% of trihydroxymethyl aminomethane, 31% of glucose, 12.3% of citric acid, 7.3% of fructose, 6.5% of sodium citrate, 6.1% of vitamin C, 5.5% of vitamin B, 2.2% of EDTA, 2.2% of sodium chloride, 2.8% of compound antibiotic and the balance of double distilled water.
Further optimized, the compound antibiotic is 3000-5000 units of streptomycin.
A preparation method of a normal-temperature short-term preservation diluent of sheep fresh essence comprises the following steps:
s1, primarily mixing, namely heating double distilled water to 50 ℃ at a constant speed, adding trihydroxymethyl aminomethane, glucose, citric acid, fructose, sodium citrate, vitamin C, vitamin B, EDTA and sodium chloride into the double distilled water, uniformly stirring, filtering the mixed solution through a 0.2 um filter membrane, collecting the filtrate, and preserving at a constant temperature of 50 ℃;
s2, preparing, namely adding the compound antibiotic into the filtrate prepared in the step S1, stirring uniformly at constant temperature to obtain a finished product diluent, and then adjusting the temperature of the diluent to 38.5 ℃ and storing in dark place.
It should be noted that the diluent prepared in step S2 has a storage time of 24 hours.
A use method of a normal-temperature short-term preservation diluent for fresh sheep semen comprises the following steps:
firstly, screening sheep semen, namely firstly, adjusting the temperature of collected fresh ram semen to 37 ℃ for storage, then sampling the fresh ram semen and carrying out quality evaluation on the apparent motility rate, the motility rate and the density of the fresh ram semen through a microscope, wherein the apparent motility rate is set into a quality grade from 0 to 5 according to the fluctuation intensity, then the ram semen with the motility rate of 4 grades, the motility rate of more than 0.7 and the density of more than 20 hundred million/mL is selected as the semen to be diluted to be qualified;
and secondly, diluting, namely adding a diluent into the fresh ram semen in the preservation state selected in the first step, wherein the mixing ratio of the fresh ram semen to the diluent is 1: 10, and then transferring the mixed and diluted ram semen into a semen collection cup for preserving heat for later use.
It is important to note that when the apparent activity is classified into quality grades according to the fluctuation intensity, the 0 grade is no fluctuation, and the 5 grade is rapid vortex-like fluctuation.
In addition, the diluted sheep semen solution is preserved for 24 hours.
Example 3
As shown in fig. 1-4, a normal-temperature short-term preservation diluent of sheep fresh essence comprises the following components in percentage by mass: 18% of trihydroxymethyl aminomethane, 25% of glucose, 9.5% of citric acid, 4.3% of fructose, 4.8% of sodium citrate, 5.5% of vitamin C, 3.8% of vitamin B, 1.8% of EDTA, 2.0% of sodium chloride, 1.7% of compound antibiotic and the balance of double distilled water.
In this example, the antibiotic complex is 4000 units of streptomycin.
A preparation method of a normal-temperature short-term preservation diluent of sheep fresh essence comprises the following steps:
s1, primarily mixing, namely heating double distilled water to 30 ℃ at a constant speed, adding trihydroxymethyl aminomethane, glucose, citric acid, fructose, sodium citrate, vitamin C, vitamin B, EDTA and sodium chloride into the double distilled water, uniformly stirring, filtering the mixed solution through a 0.2 um filter membrane, collecting the filtrate, and preserving the filtrate at a constant temperature of 40 ℃;
s2, preparing, namely adding the compound antibiotic into the filtrate prepared in the step S1, stirring uniformly at constant temperature to obtain a finished product diluent, and then adjusting the temperature of the diluent to 37 ℃ and storing in a dark place.
In this example, the time for which the diluent prepared in step S2 was stored was 12 hours.
A use method of a normal-temperature short-term preservation diluent for fresh sheep semen comprises the following steps:
firstly, screening sheep semen, namely firstly, adjusting the temperature of collected fresh ram semen to 37 ℃ for storage, then sampling the fresh ram semen and carrying out quality evaluation on the apparent motility rate, the motility rate and the density of the fresh ram semen through a microscope, wherein the apparent motility rate is set into a quality grade from 0 to 5 according to the fluctuation intensity, then the ram semen with the motility rate of 4 grades, the motility rate of more than 0.7 and the density of more than 20 hundred million/mL is selected as the semen to be diluted to be qualified;
and secondly, diluting, namely adding a diluent into the fresh ram semen in the preservation state selected in the first step, wherein the mixing ratio of the fresh ram semen to the diluent is 1:6, and then transferring the mixed and diluted ram semen into a semen collection cup for preserving heat for later use.
Meanwhile, when the apparent activity rate is divided into quality grades according to the fluctuation intensity, the grade 0 is no fluctuation, and the grade 5 is rapid vortex-shaped fluctuation.
In addition, the diluted sheep semen solution is preserved for 14 hours.
The sheep semen diluent and the preparation method thereof have low production cost, and on one hand, the sheep semen diluent can effectively improve the utilization efficiency of stud rams, increase the coverage rate of fine breeds, reduce the configuration of artificial insemination personnel and improve the labor efficiency. Meanwhile, the conception rate of artificial insemination and the efficiency of in vitro insemination are effectively improved, and the method is convenient for large-scale popularization and application.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (7)
1. The normal-temperature short-term preservation diluent for the sheep fresh essence is characterized by comprising the following components in parts by weight: the normal-temperature short-term preservation diluent for the sheep fresh essence comprises the following components in percentage by mass: 11% -24% of tris (hydroxymethyl) aminomethane, 13% -31% of glucose, 5% -12.3% of citric acid, 3% -7.3% of fructose, 3% -6.5% of sodium citrate, 2.5% -6.1% of vitamin C, 2.2% -5.5% of vitamin B, 1.2% -2.2% of EDTA, 1.9% -2.2% of sodium chloride, 0.5% -2.8% of compound antibiotic and the balance of double distilled water.
2. The normal-temperature short-term preservation diluent for the sheep fresh essence according to claim 1 is characterized in that: the compound antibiotic is penicillin streptomycin with 3000-5000 units.
3. A preparation method of a normal-temperature short-term preservation diluent of sheep fresh essence is characterized by comprising the following steps: the preparation method of the normal-temperature short-term preservation diluent for the fresh sheep semen comprises the following steps:
s1, primarily mixing, namely heating double distilled water to 20-50 ℃ at a constant speed, adding trihydroxymethyl aminomethane, glucose, citric acid, fructose, sodium citrate, vitamin C, vitamin B, EDTA and sodium chloride into the double distilled water, uniformly stirring, filtering the mixed solution through a 0.2-micron filter membrane, collecting the filtrate, and preserving the filtrate at a constant temperature of 20-50 ℃;
s2, preparing, namely adding the compound antibiotic into the filtrate prepared in the step S1, stirring uniformly at constant temperature to obtain a finished product diluent, and then adjusting the temperature of the diluent to 30-38.5 ℃ and storing.
4. The preparation method of the sheep fresh essence normal-temperature short-term preservation diluent according to claim 3 is characterized by comprising the following steps: the time for storing the diluent prepared in the step S2 is 5-24 hours.
5. A using method of a normal-temperature short-term preservation diluent of sheep fresh essence is characterized by comprising the following steps: the using method of the normal-temperature short-term preservation diluent for the fresh sheep semen comprises the following steps:
firstly, screening sheep semen, namely firstly, adjusting the temperature of collected fresh ram semen to 37 ℃ for storage, then sampling the fresh ram semen and carrying out quality evaluation on the apparent motility rate, the motility rate and the density of the fresh ram semen through a microscope, wherein the apparent motility rate is set into a quality grade from 0 to 5 according to the fluctuation intensity, then the ram semen with the motility rate of 4 grades, the motility rate of more than 0.7 and the density of more than 20 hundred million/mL is selected as the semen to be diluted to be qualified;
and secondly, diluting, namely adding a diluent into the fresh ram semen in the preservation state selected in the first step, wherein the mixing ratio of the fresh ram semen to the diluent is 1: 5-10, and then transferring the mixed and diluted sheep semen to a semen collection cup for heat preservation and standby.
6. The preparation method of the sheep fresh essence normal-temperature short-term preservation diluent, according to claim 5, is characterized by comprising the following steps: when the apparent activity rate is divided into quality grades according to the fluctuation intensity, the 0 grade is no fluctuation, and the 5 grade is rapid vortex-shaped fluctuation.
7. The preparation method of the sheep fresh essence normal-temperature short-term preservation diluent, according to claim 5, is characterized by comprising the following steps: the preservation time of the diluted sheep semen is 1-24 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011506138.0A CN112514890A (en) | 2020-12-18 | 2020-12-18 | Normal-temperature short-term preservation diluent for fresh sheep essence and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011506138.0A CN112514890A (en) | 2020-12-18 | 2020-12-18 | Normal-temperature short-term preservation diluent for fresh sheep essence and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112514890A true CN112514890A (en) | 2021-03-19 |
Family
ID=75001863
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011506138.0A Pending CN112514890A (en) | 2020-12-18 | 2020-12-18 | Normal-temperature short-term preservation diluent for fresh sheep essence and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112514890A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3973003A (en) * | 1973-12-18 | 1976-08-03 | Institut National De La Recherche Agronomique | Process for the preservation of ram semen by freezing |
| CN103518706A (en) * | 2013-10-21 | 2014-01-22 | 西北农林科技大学 | Diluent of frozen semen of milk goat, and preparation method and diluting method thereof as well as preparation method of frozen semen of milk goat in thin pipe |
| CN103548812A (en) * | 2013-10-30 | 2014-02-05 | 西北农林科技大学 | Normal-temperature and low-temperature preservation diluents of milk goat seminal fluid |
| CN104068013A (en) * | 2014-07-17 | 2014-10-01 | 赤峰市农牧科学研究院 | Sheep semen dilution preserving fluid and preparation method thereof |
| CN109006802A (en) * | 2018-07-08 | 2018-12-18 | 乌鲁木齐九品芝麻信息科技有限公司 | Store method when a kind of fresh essence of sheep is long |
-
2020
- 2020-12-18 CN CN202011506138.0A patent/CN112514890A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3973003A (en) * | 1973-12-18 | 1976-08-03 | Institut National De La Recherche Agronomique | Process for the preservation of ram semen by freezing |
| CN103518706A (en) * | 2013-10-21 | 2014-01-22 | 西北农林科技大学 | Diluent of frozen semen of milk goat, and preparation method and diluting method thereof as well as preparation method of frozen semen of milk goat in thin pipe |
| CN103548812A (en) * | 2013-10-30 | 2014-02-05 | 西北农林科技大学 | Normal-temperature and low-temperature preservation diluents of milk goat seminal fluid |
| CN104068013A (en) * | 2014-07-17 | 2014-10-01 | 赤峰市农牧科学研究院 | Sheep semen dilution preserving fluid and preparation method thereof |
| CN109006802A (en) * | 2018-07-08 | 2018-12-18 | 乌鲁木齐九品芝麻信息科技有限公司 | Store method when a kind of fresh essence of sheep is long |
Non-Patent Citations (1)
| Title |
|---|
| 朱兴贵等主编: "《畜禽繁育技术》", 31 July 2015, 中国轻工业出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103518706B (en) | Diluent of frozen semen of milk goat, and preparation method and diluting method thereof as well as preparation method of frozen semen of milk goat in thin pipe | |
| CN103548812B (en) | Normal-temperature and low-temperature preservation diluents of milk goat seminal fluid | |
| CN110074096B (en) | Serum-free cell cryopreservation liquid and preparation method and application thereof | |
| CN103299987A (en) | Diluent for normal temperature preservation of boar semens and preparation method thereof | |
| CN100581553C (en) | Diluent for preserving frozen red deer semen | |
| CN112369409A (en) | Basic diluent of frozen pig semen as well as preparation method and application thereof | |
| CN104585164A (en) | Improved diluent for long-term preservation of semen of ruminant | |
| CN103222458A (en) | Swine gender-controlling sperm and preparation method and application thereof | |
| CN109825442A (en) | A kind of ferment liquid fertilizer and its preparation method and application | |
| CN110959607A (en) | Formula and dilution method of diluent for storing Hu sheep semen at normal temperature | |
| CN112514890A (en) | Normal-temperature short-term preservation diluent for fresh sheep essence and preparation method thereof | |
| CN110074093B (en) | Programmed cooling and freezing method for human umbilical cord blood hematopoietic stem cells | |
| CN105850978A (en) | Boar semen room-temperature preservation dilution powder and diluent, and application of dilution powder and diluent | |
| CN108684654A (en) | A kind of optimization dilution | |
| CN111657270B (en) | Normal-temperature preservative for livestock semen and preparation method and use method thereof | |
| CN114788516A (en) | Sheep semen diluent and preparation method thereof | |
| CN110684721B (en) | Method for detecting embryo quality by using culture medium | |
| Tuba et al. | On staining for vitamin C in tissues | |
| CN106993605A (en) | A kind of down producing goat freezing seminal fluid dilution formula | |
| CN111066776A (en) | Ovum freezing protective agent and application thereof | |
| CN112586494A (en) | Fried smoking method frozen beef sperm manufacturing process | |
| CN102657150B (en) | Formula of sika deer tubule frozen sperm diluent containing ginseng polysaccharide and application method of formula | |
| AU2021102920A4 (en) | Base diluent for porcine frozen semen and preparation method and application thereof | |
| CN109907170A (en) | A kind of compound anti-coagulants of animal blood | |
| CN117158414B (en) | Pig sperm cryopreservation diluent containing safflower polysaccharide and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210319 |
|
| RJ01 | Rejection of invention patent application after publication |