CN112501021A

CN112501021A - Stem cell amplification equipment

Info

Publication number: CN112501021A
Application number: CN202011430257.2A
Authority: CN
Inventors: 杨慧慧
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-12-07
Filing date: 2020-12-07
Publication date: 2021-03-16

Abstract

The invention relates to the technical field of stem cell amplification, in particular to stem cell amplification equipment which comprises a workbench, a separation device, an extraction box, a transfer device, a culture dish and a culture solution spraying device, wherein the workbench is arranged on a horizontal plane, the separation device is arranged on the workbench, the extraction box is arranged on the surface of the workbench and is positioned beside the separation device, the transfer device is arranged on the workbench and is positioned on the opposite side of the extraction box, the culture dish is arranged on the workbench and is positioned beside the transfer device, the culture solution spraying device is arranged on the workbench, a spraying end of the culture solution spraying device extends into the culture dish, the separation device is used for separating stem cells in blood, the extraction box is used for extracting the stem cells, the transfer device is used for transferring the extracted stem cells into the culture dish, and the culture solution spraying device is used for uniformly spraying culture solution on the surface of the stem cells, is beneficial to the expansion of the stem cells.

Description

Stem cell amplification equipment

Technical Field

The invention relates to the technical field of stem cell amplification, in particular to stem cell amplification equipment.

Background

For example, patent publication No. CN109290114A relates to a novel wheel electroplating spraying device for stem cell amplification device; the utility model discloses a stem cell amplification equipment, including bottle, connection shell, bottle lid, pushing mechanism, sealing mechanism, pushing mechanism, rubber mat, pressure spring, fore-set, notes liquid mechanism, push rod, push pedal, play liquid groove, sealed post, reset spring and connecting plate. The utility model has the advantages that: the utility model discloses a bottom sliding connection of bottle has pushing mechanism, press the base when pouring into the nutrient solution into to the inside of bottle and realized the pressure spring shrink, the fore-set removes to the bottle lid direction, and then realized the push rod and removed the push pedal to the lateral wall of closing plate, and then realized the reset spring shrink, the drain tank is opened by sealed post, it reaches the inside of bottle to be located the inside nutrient solution of guard band, rubber mat takes place deformation when nutrient solution reaches bottle inside, stem cell flows on the surface of rubber mat, the comprehensive contact of stem cell with the nutrient solution has been realized, do benefit to the amplification of stem cell, stem cell has been avoided breaking. However, the above-mentioned devices also have the following problems in use: firstly, during large-scale culture, cells need to be changed every two days, and need passage about four days, and these daily maintenance operations need to consume a large amount of time and human cost, and the cost of labor is high, and secondly, in long-time culture process, need to open the blake bottle repeatedly and change liquid, passage operation, this greatly increased the risk of pollution.

Therefore, it is highly desirable to develop a stem cell expansion device with good expansion effect and convenient operation.

Disclosure of Invention

The technical problem to be solved by the invention is to provide stem cell amplification equipment to solve the problems that in the prior art, during large-scale culture, cells need to be changed every two days, about four days, passage is needed, a large amount of time and labor cost are consumed for daily maintenance operation, the labor cost is high, and in a long-time culture process, a culture bottle needs to be repeatedly opened for liquid change and passage operation, so that the risk of pollution is greatly increased.

In order to solve the technical problems, the invention provides the following technical scheme: a stem cell amplification device comprises a workbench, a separation device, an extraction box, a transfer device, a culture dish and a culture solution spraying device, wherein the workbench is arranged on a horizontal plane, the separation device is arranged on the workbench, the extraction box is arranged on the surface of the workbench and located beside the separation device, the transfer device is arranged on the workbench and located on the opposite side of the extraction box, the culture dish is arranged on the workbench and located beside the transfer device, the culture solution spraying device is arranged on the workbench, and the spraying end of the culture solution spraying device extends into the culture dish;

draw the case and include first filter layer, second filter layer, third filter layer and abandonment blood collection layer, first filter layer, second filter layer, third filter layer and abandonment blood collection layer set up and draw incasement portion and set up from the top down in proper order, the third filter layer with draw the inside wall sliding fit of case, first filter layer is the adsorption column net that the diameter is 100 ~ 120 mu m, the second filter layer is the adsorption column net that the diameter is 60 ~ 80 mu m, the third filter layer is the adsorption column net that the diameter is 40 ~ 50 mu m.

Furthermore, the transfer assembly comprises a transfer motor, a second gear, a third gear, a second rotating shaft, a rotating plate, a transfer cylinder, a connecting rod and two suckers, the transfer motor is fixedly arranged on the workbench, the second gear is connected with the output end of the transfer motor, the second gear and the third gear are meshed with each other, the second rotating shaft is connected with the third gear, the rotating plate is connected with the second rotating shaft, the transfer cylinder is fixedly arranged on the rotating plate, the connecting rod is connected with the output end of the transfer cylinder, the number of the suckers is two, and the two suckers are respectively connected with the connecting rod.

Further, the culture solution spraying device includes culture solution bin, first mount pad, conveyer pipe, extraction pump, inlet tube and shower nozzle, the culture solution bin sets up on the workstation, first mount pad sets up on the lateral wall of culture dish, the extraction pump sets up on the first mount pad, the one end of conveyer pipe is connected on the culture solution bin, the other end of conveyer pipe is connected on the extraction pump input, the one end of inlet tube is connected on the output of extraction pump, the other end of inlet tube runs through the inside that the lateral wall of culture dish extends to the culture dish, the shower nozzle is equipped with a plurality of, and a plurality of shower nozzle is equidistant to be set up on the inlet tube.

Furthermore, be equipped with the door of switch on the culture dish, be equipped with the mounting bracket of putting stem cell in the culture dish, be equipped with the discharging pipe on the lateral wall of culture dish, be equipped with solenoid valve on the discharging pipe.

Compared with the prior art, the invention has the beneficial effects that:

firstly, impurities on the surface are filtered through a first filtering layer and a second filtering layer, the filtered stem cells fall onto a third filtering layer, and then the blood after extraction flows into a waste blood collecting layer.

And secondly, drive the second gear through shifting the motor and rotate, second gear and third gear intermeshing drive the second axis of rotation and rotate, the second axis of rotation drives the rotor plate and rotates, drive connecting rod and two sucking disc work through shifting the cylinder, shift stem cell and carry out expansion on next step in shifting the culture dish, the setting through shifting the subassembly need not the staff and shifts to the culture dish to the stem cell who has drawed, simple swift also safer simultaneously, has improved the practicality of this device greatly.

And thirdly, when the stem cells are transferred to the culture dish, the culture solution in the culture solution storage tank is extracted to the delivery pipe by the extraction pump, the culture solution is delivered to the water inlet pipe through the delivery pipe, and the water inlet pipe enables the stem cells to be better expanded through the surface of the stem cells uniformly sprayed with the culture solution through a plurality of spray heads.

Fourthly, when the transfer device transfers the stem cells to the mounting bracket in the culture dish, the door on the culture dish is closed, after the culture solution is sprayed and finished, the electromagnetic valve is switched on, and the waste liquid after being sprayed is discharged.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a perspective sectional view of the present invention;

FIG. 3 is a schematic perspective view of the stirring assembly of the present invention;

FIG. 4 is a schematic perspective view of the lift assembly of the present invention;

FIG. 5 is a schematic perspective view of the transfer device of the present invention;

FIG. 6 is a perspective sectional view of the extraction box of the present invention;

FIG. 7 is a schematic perspective view of a culture solution spraying apparatus according to the present invention.

The reference numbers in the figures are:

the working table 1, the separating device 2, the separating box 21, the transverse sliding table 211, the platform 212, the first motor 213, the first gear 214, the first rack 215, the feed inlet 216, the connecting pipe 217, the lifting assembly 22, the supporting plate 221, the lifting plate 222, the lifting cylinder 223, the adjusting clamping strip 224, the adjusting connecting rod 225, the adjusting gear 226, the stirring assembly 23, the second motor 231, the first rotating shaft 232, the stirring shaft 233, the stirring blade 234, the extraction box 3, the first filter layer 31, the second filter layer 32, the third filter layer 33, the waste blood collecting layer 34, the transferring device 4, the transferring motor 41, the second gear 42, the third gear 43, the second rotating shaft 44, the rotating plate 45, the transferring cylinder 46, the connecting rod 47, the suction cup 48, the culture dish 5, the door 51, the mounting rack 52, the discharge pipe 53, the electromagnetic valve 54, the culture solution spraying device 6, the culture solution storage box 61, the first mounting seat 62, a delivery pipe 63, a suction pump 64, a water inlet pipe 65 and a spray head 66.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

Referring to fig. 1 to 7, a stem cell amplification apparatus is disclosed, which includes a workbench 1, a separation device 2, an extraction box 3, a transfer device 4, a culture dish 5 and a culture solution spraying device 6, wherein the workbench 1 is disposed on a horizontal plane, the separation device 2 is disposed on the workbench 1, the extraction box 3 is disposed on the surface of the workbench 1 and beside the separation device 2, the transfer device 4 is disposed on the workbench 1 and at the opposite side of the extraction box 3, the culture dish 5 is disposed on the workbench 1 and beside the transfer device 4, the culture solution spraying device 6 is disposed on the workbench 1, and the spraying end of the culture solution spraying device 6 extends into the culture dish 5; the separation device 2 separates stem cells in blood, the extraction box 3 extracts the stem cells, the transfer device 4 transfers the extracted stem cells to the culture dish 5, and the culture solution spraying device 6 uniformly sprays culture solution on the surfaces of the stem cells, so that the stem cells can be beneficially amplified.

The separating device 2 comprises a separating box 21, a lifting component 22 and a stirring component 23, the separating box 21 is fixedly arranged on the workbench 1, the separation box 21 is provided with a feed inlet 216 and a connecting pipe 217, the feed inlet 216 is fixedly arranged at the top end of the separation box 21, one end of the connecting pipe 217 is connected with the bottom end of the separation tank 21, the other end of the connecting pipe 217 is connected with the extraction tank 3, a transverse sliding table 211 and a platform 212 are further arranged in the separation box 21, the transverse sliding table 211 is fixedly arranged on the top wall in the separation box 21, the platform 212 is arranged on the transverse sliding table 211, the platform 212 is provided with a first motor 213, the output end of the first motor 213 is provided with a first gear 214, a first rack 215 is arranged on the rear side of the transverse sliding table 211, two groups of lifting assemblies 22 are arranged, the two groups of lifting assemblies 22 are arranged in the separation box 21, and the stirring assembly 23 is fixedly arranged at the working end of the lifting assembly 22; put into feed inlet 216 with the raw materials, first motor 213 drives first gear 214 rotatory, first gear 214 and the meshing of first rack 215 drive platform 212 horizontal migration on horizontal slip table 211 to drive stirring subassembly 23 and lifting unit 22 simultaneously and remove, lifting unit 22 drives stirring subassembly 23 and goes up and down, stirring subassembly 23 realizes stem cell separation work, after the separation is accomplished, connecting pipe 217 is carried the raw materials that separate to in extracting box 3.

The lifting assembly 22 comprises a supporting plate 221, a lifting plate 222, a lifting cylinder 223, adjusting clamping strips 224, an adjusting connecting rod 225 and an adjusting gear 226, wherein the supporting plate 221 and the lifting plate 222 are both in a T shape, the supporting plate 221 and the lifting plate 222 are oppositely arranged up and down, the lifting cylinder 223 is fixedly arranged on one side of the supporting plate 221, an adjusting groove is formed in the supporting plate 221, two groups of adjusting clamping strips 224 are arranged, the two groups of adjusting clamping strips 224 are both in a Z shape, the two groups of adjusting clamping strips 224 are respectively arranged on the supporting plate 221 and the lifting plate 222, the adjusting clamping strips 224 on the supporting plate 221 are slidably arranged on the adjusting groove, the output end of the lifting cylinder 223 is connected with the adjusting clamping strips 224 on the supporting plate 221, the adjusting clamping strips 224 on the lifting plate 222 are fixedly arranged on the lifting plate 222, and the adjusting gear 226 is provided with four groups, the four groups of adjusting gears 226 are respectively arranged at two ends of the two groups of adjusting clamping strips 224, the four groups of adjusting gears 226 are respectively meshed with the adjusting clamping strips 224, two groups of adjusting connecting rods 225 are arranged, and two ends of the two groups of adjusting connecting rods 225 are respectively rotatably connected with the four groups of adjusting gears 226; the lifting cylinder 223 drives the two sets of adjusting clamping strips 224 to move, the two sets of adjusting clamping strips 224 are respectively arranged on the supporting plate 221 and the lifting plate 222, the four sets of adjusting gears 226 are meshed with the two sets of adjusting clamping strips 224, the lifting plate 222 is driven to lift immediately, the lifting plate 222 drives the stirring assembly 23 to lift, and stem cells are better separated.

The stirring assembly 23 comprises a second motor 231, a first rotating shaft 232, a stirring shaft 233 and stirring blades 234, the second motor 231 is fixedly arranged on the lifting assembly 22, the first rotating shaft 232 is connected with the output end of the second motor 231, the stirring shaft 233 is connected with the first rotating shaft 232, and the stirring blades 234 are fixedly arranged on the stirring shaft 233; the second motor 231 drives the first rotating shaft 232 to rotate, the first rotating shaft 232 drives the stirring shaft 233 to rotate, and the stirring shaft 233 drives the stirring blades 234 to rotate, so that the stem cells are separated.

The extraction box 3 comprises a first filter layer 31, a second filter layer 32, a third filter layer 33 and a waste blood collection layer 34, wherein the first filter layer 31, the second filter layer 32, the third filter layer 33 and the waste blood collection layer 34 are arranged inside the extraction box 3 and are sequentially arranged from top to bottom, the third filter layer 33 is in sliding fit with the inner side wall of the extraction box 3, the first filter layer 31 is an adsorption column grid with the diameter of 100-120 mu m, the second filter layer 32 is an adsorption column grid with the diameter of 60-80 mu m, and the third filter layer 33 is an adsorption column grid with the diameter of 40-50 mu m; the impurities on the surface are filtered by the first filter layer 31 and the second filter layer 32, and the filtered stem cells fall onto the third filter layer 33, and then the blood after completion of extraction flows into the waste blood collecting layer 34.

The transfer device 4 comprises a transfer motor 41, a second gear 42, a third gear 43, a second rotating shaft 44, a rotating plate 45, a transfer cylinder 46, a connecting rod 47 and suckers 48, wherein the transfer motor 41 is fixedly arranged on the workbench 1, the second gear 42 is connected with the output end of the transfer motor 41, the second gear 42 and the third gear 43 are meshed with each other, the second rotating shaft 44 is connected with the third gear 43, the rotating plate 45 is connected with the second rotating shaft 44, the transfer cylinder 46 is fixedly arranged on the rotating plate 45, the connecting rod 47 is connected with the output end of the transfer cylinder 46, the suckers 48 are two in number, and the two suckers 48 are respectively connected with the connecting rod 47; drive second gear 42 through transfer motor 41 and rotate, second gear 42 and third gear 43 intermeshing drive second axis of rotation 44 and rotate, second axis of rotation 44 drives rotor plate 45 and rotates, drive connecting rod 47 and two sucking disc 48 work through transfer cylinder 46, shift the stem cell and carry out amplification on next step in culture dish 5, it shifts to culture dish 5 to the stem cell who has drawn through transfer device 4's setting need not the staff, simple swift also safer simultaneously, the practicality of this device has been improved greatly.

The culture solution spraying device 6 comprises a culture solution storage tank 61, a first mounting seat 62, a conveying pipe 63, an extraction pump 64, a water inlet pipe 65 and spray heads 66, wherein the culture solution storage tank 61 is arranged on the workbench 1, the first mounting seat 62 is arranged on the side wall of the culture dish 5, the extraction pump 64 is arranged on the first mounting seat 62, one end of the conveying pipe 63 is connected to the culture solution storage tank 61, the other end of the conveying pipe 63 is connected to the input end of the extraction pump 64, one end of the water inlet pipe 65 is connected to the output end of the extraction pump 64, the other end of the water inlet pipe 65 penetrates through the side wall of the culture dish 5 and extends to the inside of the culture dish 5, the spray heads 66 are provided with a plurality of spray heads 66, and the spray heads 66 are arranged on the water inlet pipe 65 at; when stem cells are transferred to the culture dish 5, the culture solution in the culture solution storage tank 61 is pumped to the conveying pipe 63 by the operation of the pumping pump 64, the culture solution is conveyed to the water inlet pipe 65 through the conveying pipe 63, and the water inlet pipe 65 enables the stem cells to be better expanded by uniformly spraying the culture solution on the surfaces of the stem cells through the plurality of spray heads 66.

A door 51 capable of being opened and closed is arranged on the culture dish 5, an installation frame 52 for placing stem cells is arranged in the culture dish 5, a discharge pipe 53 is arranged on the side wall of the culture dish 5, and an electromagnetic valve 54 is arranged on the discharge pipe 53; when the stem cells are transferred to the mounting frame 52 in the culture dish 5 by the transfer device 4, the door 51 on the culture dish 5 is closed, and after the culture solution is sprayed, the electromagnetic valve 54 is turned on, and the sprayed waste solution is discharged.

The working principle is as follows: the raw material is put into the feed inlet 216, the first motor 213 drives the first gear 214 to rotate, the first gear 214 is meshed with the first rack 215 to drive the platform 212 to horizontally move on the transverse sliding table 211, so as to drive the stirring assembly 23 and the lifting assembly 22 to move simultaneously, the lifting cylinder 223 drives the two sets of adjusting clamping strips 224 to move, the two sets of adjusting clamping strips 224 are respectively arranged on the supporting plate 221 and the lifting plate 222, the four sets of adjusting gears 226 are meshed with the two sets of adjusting clamping strips 224 to drive the lifting plate 222 to lift, the lifting plate 222 drives the stirring assembly 23 to lift, better separate the dry cells, the second motor 231 drives the first rotating shaft 232 to rotate, the first rotating shaft 232 drives the stirring shaft 233 to rotate, the stirring shaft 233 drives the stirring blades 234 to rotate to realize the separation of the dry cells, after the separation is finished, the separated raw material is conveyed into the extraction box 3 by the connecting pipe 217, the impurities on the surface are filtered through the first filter layer 31 and the second filter layer 32, the filtered stem cells fall onto the third filter layer 33, then the blood after extraction is flowed into the waste blood collection layer 34, the second gear 42 is driven to rotate through the transfer motor 41, the second gear 42 and the third gear 43 are meshed with each other to drive the second rotating shaft 44 to rotate, the second rotating shaft 44 drives the rotating plate 45 to rotate, the connecting rod 47 and the two suckers 48 are driven to work through the transfer cylinder 46, the stem cells are transferred into the culture dish 5 for next amplification, the extracted stem cells are transferred onto the mounting frame 52 in the culture dish 5 through the arrangement of the transfer device 4 without a worker, when the stem cells are transferred into the culture dish 5, the culture solution in the culture solution storage tank 61 is extracted into the conveying pipe 63 through the conveying pipe 63, the culture solution is conveyed into the water inlet pipe 65 through the conveying pipe 63, the water inlet pipe 65 makes the stem cells better expand through the surface of the stem cells evenly sprayed with the culture solution by a plurality of spray heads 66, and after the culture solution is sprayed and completed, the electromagnetic valve 54 is switched on, and the waste liquid after being sprayed is discharged.

Claims

1. A stem cell expansion apparatus, comprising: the culture dish device comprises a workbench (1), a separating device (2), an extracting box (3), a transferring device (4), a culture dish (5) and a culture solution spraying device (6), wherein the workbench (1) is arranged on a horizontal plane, the separating device (2) is arranged on the workbench (1), the extracting box (3) is arranged on the surface of the workbench (1) and is positioned at the side of the separating device (2), the transferring device (4) is arranged on the workbench (1) and is positioned at the opposite side of the extracting box (3), the culture dish (5) is arranged on the workbench (1) and is positioned at the side of the transferring device (4), the culture solution spraying device (6) is arranged on the workbench (1), and the spraying end of the culture solution spraying device (6) extends into the culture dish (5);

draw case (3) including first filter layer (31), second filter layer (32), third filter layer (33) and abandonment blood collection layer (34), first filter layer (31), second filter layer (32), third filter layer (33) and abandonment blood collection layer (34) set up and draw case (3) inside and from the top down setting in proper order, third filter layer (33) and the inside wall sliding fit who draws case (3), first filter layer (31) are the adsorption column net that the diameter is 100 ~ 120 mu m, second filter layer (32) are the adsorption column net that the diameter is 60 ~ 80 mu m, third filter layer (33) are the adsorption column net that the diameter is 40 ~ 50 mu m.

2. A stem cell expansion apparatus according to claim 1, wherein: the transfer device (4) comprises a transfer motor (41), a second gear (42), a third gear (43), a second rotating shaft (44), a rotating plate (45), a transfer cylinder (46), a connecting rod (47) and a sucker (48), the transfer motor (41) is fixedly arranged on the workbench (1), the second gear (42) is connected with the output end of the transfer motor (41), the second gear (42) and the third gear (43) are meshed with each other, the second rotating shaft (44) is connected with the third gear (43), the rotating plate (45) is connected with a second rotating shaft (44), the transfer cylinder (46) is fixedly arranged on the rotating plate (45), the connecting rod (47) is connected with the output end of the transfer cylinder (46), the number of the suckers (48) is two, and the suckers (48) are respectively connected with the connecting rod (47).

3. A stem cell expansion apparatus according to claim 1, wherein: the culture solution spraying device (6) comprises a culture solution storage tank (61), a first mounting seat (62), a delivery pipe (63), a suction pump (64), a water inlet pipe (65) and a spray head (66), the culture solution storage tank (61) is arranged on the workbench (1), the first mounting seat (62) is arranged on the side wall of the culture dish (5), the extraction pump (64) is arranged on the first mounting seat (62), one end of the delivery pipe (63) is connected on the culture solution storage tank (61), the other end of the delivery pipe (63) is connected with the input end of the extraction pump (64), one end of the water inlet pipe (65) is connected with the output end of the extraction pump (64), the other end of the water inlet pipe (65) penetrates through the side wall of the culture dish (5) and extends to the interior of the culture dish (5), the spray head (66) is provided with a plurality of spray heads (66), and the plurality of spray heads (66) are arranged on the water inlet pipe (65) at equal intervals.

4. A stem cell expansion apparatus according to claim 1, wherein: be equipped with door (51) of switch on culture dish (5), be equipped with in culture dish (5) and put stem cell's mounting bracket (52), be equipped with discharging pipe (53) on the lateral wall of culture dish (5), be equipped with solenoid valve (54) on discharging pipe (53).