CN112500998B - Safe and environment-friendly crushing and centrifuging device and method for preparing nitrilase - Google Patents
Safe and environment-friendly crushing and centrifuging device and method for preparing nitrilase Download PDFInfo
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- CN112500998B CN112500998B CN202011471654.4A CN202011471654A CN112500998B CN 112500998 B CN112500998 B CN 112500998B CN 202011471654 A CN202011471654 A CN 202011471654A CN 112500998 B CN112500998 B CN 112500998B
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- 108010033272 Nitrilase Proteins 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 15
- 230000001580 bacterial effect Effects 0.000 claims abstract description 27
- 230000005540 biological transmission Effects 0.000 claims description 44
- 230000007246 mechanism Effects 0.000 claims description 20
- 239000000725 suspension Substances 0.000 claims description 8
- 238000000703 high-speed centrifugation Methods 0.000 claims description 7
- 230000009471 action Effects 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 5
- 230000008878 coupling Effects 0.000 claims description 2
- 238000010168 coupling process Methods 0.000 claims description 2
- 238000005859 coupling reaction Methods 0.000 claims description 2
- 238000004891 communication Methods 0.000 claims 1
- 230000010354 integration Effects 0.000 abstract description 6
- -1 Nitrile compounds Chemical class 0.000 description 4
- XLJMAIOERFSOGZ-UHFFFAOYSA-N cyanic acid Chemical compound OC#N XLJMAIOERFSOGZ-UHFFFAOYSA-N 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- JFDZBHWFFUWGJE-UHFFFAOYSA-N benzonitrile Chemical compound N#CC1=CC=CC=C1 JFDZBHWFFUWGJE-UHFFFAOYSA-N 0.000 description 3
- DMCPFOBLJMLSNX-UHFFFAOYSA-N indole-3-acetonitrile Chemical compound C1=CC=C2C(CC#N)=CNC2=C1 DMCPFOBLJMLSNX-UHFFFAOYSA-N 0.000 description 3
- GZPHSAQLYPIAIN-UHFFFAOYSA-N 3-pyridinecarbonitrile Chemical compound N#CC1=CC=CN=C1 GZPHSAQLYPIAIN-UHFFFAOYSA-N 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 2
- 239000000543 intermediate Substances 0.000 description 2
- 150000002825 nitriles Chemical group 0.000 description 2
- SUSQOBVLVYHIEX-UHFFFAOYSA-N phenylacetonitrile Chemical compound N#CCC1=CC=CC=C1 SUSQOBVLVYHIEX-UHFFFAOYSA-N 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- QBYIENPQHBMVBV-HFEGYEGKSA-N (2R)-2-hydroxy-2-phenylacetic acid Chemical compound O[C@@H](C(O)=O)c1ccccc1.O[C@@H](C(O)=O)c1ccccc1 QBYIENPQHBMVBV-HFEGYEGKSA-N 0.000 description 1
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 241000589291 Acinetobacter Species 0.000 description 1
- NLHHRLWOUZZQLW-UHFFFAOYSA-N Acrylonitrile Chemical compound C=CC#N NLHHRLWOUZZQLW-UHFFFAOYSA-N 0.000 description 1
- 241000588986 Alcaligenes Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- IWYDHOAUDWTVEP-UHFFFAOYSA-N R-2-phenyl-2-hydroxyacetic acid Natural products OC(=O)C(O)C1=CC=CC=C1 IWYDHOAUDWTVEP-UHFFFAOYSA-N 0.000 description 1
- 241000316848 Rhodococcus <scale insect> Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003617 indole-3-acetic acid Substances 0.000 description 1
- 239000003262 industrial enzyme Substances 0.000 description 1
- 229960002510 mandelic acid Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
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- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
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- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/05—Means for pre-treatment of biological substances by centrifugation
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
- C12N1/066—Lysis of microorganisms by physical methods
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
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- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/05—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in nitriles (3.5.5)
- C12Y305/05001—Nitrilase (3.5.5.1)
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Abstract
The invention discloses a safe and environment-friendly crushing and centrifuging device and method for preparing nitrilase, and belongs to the technical field of nitrilase preparation. According to the crushing and centrifuging device and method for preparing the nitrilase, provided by the invention, bacterial cells are crushed by the first rotary crushing frame and the second rotary crushing frame which are opposite in rotation direction, and meanwhile, the centrifugal box is rotated at a high speed by rotating the rotary table at a high speed, so that bacterial cells crushed in the crushing bin are subjected to centrifugal operation under the centrifugal force of high-speed rotation, the crushing and centrifuging integration can be realized, the time of crushing and centrifuging is greatly reduced, and the efficiency of crushing and centrifuging is improved.
Description
Technical Field
The invention relates to the technical field of nitrilase preparation, in particular to a safe and environment-friendly crushing and centrifuging device and method for nitrilase preparation.
Background
Nitrile compounds are a class of chemical substances (R-CN) containing nitrile functional groups, and are important synthetic raw materials. They are of a wide variety and are commonly known as cyanic acid, acetonitrile, acrylonitrile, benzonitrile, phenylacetonitrile, 3-cyanopyridine, 3-indoleacetonitrile, cyanic acid and the like. The nitrile compounds can be used for obtaining compounds with higher values of amide, acid, ester and the like and wider application range, and are widely applied to chemical raw materials, medical intermediates and vitamin precursors. Nitrilase was originally isolated from barley leaves in 1964 by Thiman and Mahadevan and was able to hydrolyze indoleacetonitrile to indoleacetic acid, hence the name indoleacetonitrile hydrolase. However, in subsequent studies, the pure enzyme was found to be active against 26 different nitrile compounds, and its activity to hydrolyze 3-cyanopyridine was 8 times that of indoacetonitrile. Thiman and Mahadevan therefore designate them as nitrilases.
Nitrilase (Nitrilase, EC 3.5.5.1) is an important class of industrial enzymes belonging to the Nitrilase superfamily. Not only can degrade nitrile pollutants, but also can convert the low-cost nitrile compounds into intermediates of high-added-value industrial, food, medicine and other products, such as: acrylic acid, nicotinic acid, chiral mandelic acid, and the like. Since the 60 s of the 20 th century, the U.S. scientist Robinson and colleagues discovered the first nitrilase strain in the world, and people have been isolated to identify nitrilases of various origins, for example: rhodococcus, alcaligenes, pseudomonas, acinetobacter, and the like.
When the nitrilase is prepared, bacterial strain cells are required to be crushed and centrifuged to obtain suspension, most of the conventional crushing and centrifuging devices for the nitrilase preparation are crushed, and the bacterial strain cells are taken out of a crushing bin and then are transferred into a centrifuging device for centrifuging, so that the crushing and centrifuging integration cannot be realized, the crushing and centrifuging time is prolonged, and the crushing and centrifuging efficiency is low.
Disclosure of Invention
The invention aims to provide a crushing and centrifuging device and method for preparing nitrilase, which are safe and environment-friendly, strain cells are crushed by a first rotary crushing frame and a second rotary crushing frame with opposite rotation directions, and meanwhile, a centrifugal table rotates at a high speed to enable a centrifugal box to rotate at a high speed, so that the strain cells crushed in a crushing bin are subjected to centrifugal operation under the centrifugal force of high-speed rotation, the crushing and centrifuging integration can be realized, the time of crushing and centrifuging is greatly reduced, the efficiency of crushing and centrifuging is improved, and the problems in the background technology are solved.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the utility model provides a broken centrifugal device is used in preparation of safe and environment-friendly's nitrilase, includes the base, install the revolving stage on the base, be provided with the centrifugal box on the revolving stage, the positioning seat is all installed to the both sides terminal surface of centrifugal box, the centrifugal box is installed on the revolving stage through the fixed positioning seat of centre gripping fixed establishment centre gripping, be provided with broken storehouse in the centrifugal box, be provided with broken mechanism in the broken storehouse, broken mechanism is embedded on the centrifugal box, the just opposite face of centrifugal box still is provided with the observation window.
Further, the clamping and fixing mechanism comprises a double-shaft motor, a first transmission screw rod, a second transmission screw rod, a first translation sliding block, a second translation sliding block, a first clamping and fixing inserting block and a second clamping and fixing inserting block, wherein the double-shaft motor is installed on the inner side of the rotary table through a support, the double shafts of the double-shaft motor are respectively connected with the first transmission screw rod and the second transmission screw rod with opposite screw threads through couplings, one ends, far away from the double-shaft motor, of the first transmission screw rod and the second transmission screw rod are installed on the inner wall of the rotary table through bearings, the first translation sliding block is in threaded connection with the first transmission screw rod, the upper end of the first translation sliding block penetrates out of the rotary table to be installed with the first clamping and fixing inserting block, the upper end of the second translation sliding block penetrates out of the rotary table to be installed with the second clamping and fixing inserting block, and the first clamping and fixing inserting block and the second clamping and fixing inserting block are inserted into the positioning seat.
Further, the first translation slider and the second translation slider are both provided with guide sliding seats, the guide sliding seats are embedded on the inner wall of the rotary table, the inner wall of the rotary table is provided with strip-shaped guide grooves for the translation of the guide sliding seats, and the strip-shaped guide grooves are matched with the guide sliding seats.
Further, the crushing mechanism comprises a driving motor, a driving gear, a driven gear, a first belt pulley, a second belt pulley, a first belt, a first rotary crushing frame, a supporting shaft rod, a third belt pulley, a fourth belt pulley, a second belt and a second rotary crushing frame, wherein the driving motor is arranged on the inner side of the centrifugal box, the driving gear and the first belt pulley are arranged on an output shaft of the driving motor through a spline, the first belt pulley is connected with the second belt pulley through the first belt, the second belt pulley is arranged on the first rotary crushing frame through the spline, the first rotary crushing frame is arranged on one end of the centrifugal box, the driving gear is meshed with the driven gear, the driven gear is arranged on the supporting shaft rod through the spline, the third belt pulley is connected with the fourth belt pulley through the second belt pulley through the spline, the fourth belt pulley is arranged on the second rotary crushing frame through the spline, and the second rotary crushing frame is arranged on the other end of the centrifugal box.
Further, the first rotary crushing frame and the second rotary crushing frame comprise a rotary shaft rod, a mounting plate and a rotary crushing roller, the rotary shaft rod is mounted on the second belt pulley and the fourth belt pulley, the mounting plate is further mounted on the rotary shaft rod, and the rotary crushing roller is mounted on the mounting plate.
Further, the rotary crushing rollers are annularly distributed on the mounting plate, and the rotary crushing rollers of the first rotary crushing frame are distributed on the periphery of the rotary crushing rollers of the second rotary crushing frame.
According to another aspect of the invention, a method for crushing and centrifuging a crushing and centrifuging device for preparing a safe and environment-friendly nitrilase is provided, which comprises the following steps:
s10: the centrifugal box is placed on the rotary table, the double-shaft motor is started and drives the first transmission screw rod and the second transmission screw rod to rotate simultaneously, the first transmission screw rod and the second transmission screw rod can drive the first translation sliding block and the second translation sliding block to move oppositely under the guiding action of the guiding sliding seat in the rotating process, the first translation sliding block and the second translation sliding block can enable the first clamping fixed inserting block and the second clamping fixed inserting block to move oppositely along with the first translation sliding block and the second translation sliding block until the first clamping fixed inserting block and the second clamping fixed inserting block are inserted into the positioning seat, and at the moment, the centrifugal box is fixed on the rotary table;
s20: placing bacterial strain cells for preparing nitrilase into a crushing bin, driving a driving gear and a first belt pulley to rotate clockwise by a driving motor, enabling a first belt pulley, a second belt pulley and a first rotary crushing frame to rotate clockwise along with the driving gear, enabling a driven gear to rotate anticlockwise by the clockwise rotation of the driving gear, enabling a supporting shaft rod, a third belt pulley, a second belt, a fourth belt pulley and a second rotary crushing frame to rotate anticlockwise along with the anticlockwise rotation of the driven gear, enabling the first rotary crushing frame to rotate clockwise in the crushing bin and the second rotary crushing frame to rotate anticlockwise in the crushing bin, and crushing bacterial strain cells through the first rotary crushing frame and the second rotary crushing frame which are opposite in rotation direction;
s30: after bacterial strain cells are crushed, a driving motor embedded in a base drives a rotary table to rotate at a high speed, and the centrifugal box can rotate at a high speed along with the rotary table to make bacterial strain cells crushed in a crushing bin perform centrifugal operation under the centrifugal force of high-speed rotation, and after the bacterial strain cells are subjected to high-speed centrifugation, a crushed and centrifuged suspension for preparing nitrilase is prepared.
Compared with the prior art, the invention has the beneficial effects that:
according to the crushing and centrifuging device and method for preparing the nitrilase, the centrifugal box is fixed on the rotary table through the clamping and fixing mechanism, the driving motor is started, the first rotary crushing frame rotates clockwise in the crushing bin and the second rotary crushing frame rotates anticlockwise in the crushing bin, bacterial cells are crushed through the first rotary crushing frame and the second rotary crushing frame which rotate in opposite directions, meanwhile, the rotary table rotates at a high speed to enable the centrifugal box to rotate at a high speed, bacterial cells crushed in the crushing bin are subjected to centrifugal operation under the centrifugal force of the high speed rotation, and after the bacterial cells are subjected to high-speed centrifugation, the suspension after the crushing and centrifuging for preparing the nitrilase is prepared, so that the crushing and centrifuging integration can be realized, the time of crushing and centrifuging is greatly reduced, and the efficiency of crushing and centrifuging is improved.
Drawings
FIG. 1 is a front view of a safe and environment-friendly crushing and centrifuging device for preparing nitrilase;
FIG. 2 is a cross-sectional elevation view of the safety and environmental protection crushing centrifuge for nitrilase production of the present invention;
FIG. 3 is a cross-sectional elevation view of the mounting clamp fixture on the turntable of the present invention;
FIG. 4 is a cross-sectional elevation view of the invention with a crushing mechanism disposed within the centrifuge tank;
FIG. 5 is a front view of the crushing mechanism of the present invention;
FIG. 6 is a front view of the first and second gyratory crushing frames of the present invention in hugging and non-contacting relationship with each other;
FIG. 7 is a front view of the first and second gyratory crushing frames of the present invention not in clasping relationship with each other;
FIG. 8 is a cross-sectional view taken at A-A of FIG. 6 in accordance with the present invention;
FIG. 9 is a front view of a first rotary breaker shelf according to the invention;
FIG. 10 is a side view of a first rotary crushing frame of the present invention;
fig. 11 is a side view of a second rotary crushing frame of the invention.
In the figure: 1. a base; 2. a rotary table; 21. a strip-shaped guide groove; 3. a centrifugal box; 31. a positioning seat; 32. crushing bin; 33. an observation window; 4. a clamping and fixing mechanism; 41. a biaxial motor; 42. a first transmission screw; 43. a second transmission screw rod; 44. a first translational slide; 45. a second translation slider; 46. a first clamping and fixing insert; 47. the second clamping and fixing insert block; 5. a crushing mechanism; 51. a driving motor; 52. a drive gear; 53. a driven gear; 54. a first pulley; 55. a second pulley; 56. a first belt; 57. a first rotary crushing frame; 571. a rotating shaft; 572. a mounting plate; 573. rotating the crushing roller; 58. a support shaft; 59. a third pulley; 510. a fourth pulley; 511. a second belt; 512. a second rotary crushing frame; 6. and guiding the sliding seat.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-2, a safe and environment-friendly crushing and centrifuging device for preparing nitrilase comprises a base 1, wherein a rotary table 2 is arranged on the base 1, a centrifugal box 3 is arranged on the rotary table 2, after bacterial cells are crushed, a driving motor 51 embedded in the base 1 drives the rotary table 2 to rotate at a high speed, the rotary table 2 rotates at a high speed to enable the centrifugal box 3 to rotate at a high speed, bacterial cells crushed in a crushing bin 32 are subjected to centrifugal operation under the centrifugal force of high speed rotation, after bacterial cells are subjected to high-speed centrifugation, a suspension after crushing and centrifuging for preparing the nitrilase is prepared, positioning seats 31 are arranged on two side end surfaces of the centrifugal box 3, the centrifugal box 3 is arranged on the rotary table 2 through clamping and fixing the positioning seats 31 by a clamping and fixing mechanism 4, a crushing bin 32 is arranged in the centrifugal box 3, a crushing mechanism 5 is arranged in the crushing bin 32, the crushing mechanism 5 is embedded on the centrifugal box 3, the right opposite face of the centrifugal box 3 is also provided with an observation window 33, after the centrifugal box 3 is fixed on the rotary table 2 through the clamping and fixing mechanism 4, the driving motor 51 is started and enables the first rotary crushing frame 57 to rotate clockwise in the crushing bin 32 and the second rotary crushing frame 512 to rotate anticlockwise in the crushing bin 32, strain cells are crushed through the first rotary crushing frame 57 and the second rotary crushing frame 512 with opposite rotation directions, meanwhile, the rotary table 2 rotates at a high speed to enable the centrifugal box 3 to rotate at a high speed, strain cells crushed in the crushing bin 32 are subjected to centrifugal operation under the centrifugal force of the high-speed rotation, and after the strain cells are subjected to the high-speed centrifugation, suspension after the crushing centrifugation for preparing the nitrilase is prepared, crushing and centrifugation integration can be realized, the time of crushing centrifugation is greatly reduced, and the efficiency of the crushing centrifugation is improved.
Referring to fig. 3, the clamping and fixing mechanism 4 includes a dual-shaft motor 41, a first transmission screw 42, a second transmission screw 43, a first translation slider 44, a second translation slider 45, a first clamping and fixing insert 46 and a second clamping and fixing insert 47, put the centrifugal box 3 on the rotary table 2, the dual-shaft motor 41 starts and drives the first transmission screw 42 and the second transmission screw 43 to rotate simultaneously, the first transmission screw 42 and the second transmission screw 43 can drive the first translation slider 44 and the second translation slider 45 to move towards each other under the guiding action of the guiding slide 6, the first translation slider 44 and the second translation slider 45 move towards each other so that the first clamping and fixing insert 46 and the second clamping and fixing insert 47 also move towards each other until the first clamping and fixing insert 46 and the second clamping and fixing insert 47 are inserted into the positioning seat 31, at this moment, the centrifugal box 3 is fixed on the rotary table 2, the dual-shaft motor 41 is prevented from being separated from the rotary table 2 under high-speed rotation, the dual-shaft motor 41 is mounted on the inner side of the rotary table 2 through a support, the dual-shaft motor 41 is respectively connected with the first translation slider 44 and the second translation slider 45 through the first transmission screw 44 and the second translation slider 45 which are connected with the first translation slider 44 and the second translation slider 4 on the inner wall 2 through the first transmission slide 6, the inner wall of the first translation slider 44 is connected with the second translation slider 44, the second translation slider 44 is mounted on the inner wall of the second translation slider 4 through the opposite transmission slider 4, the inner wall of the rotary table 2 is provided with a strip-shaped guide groove 21 for the translation of the guide slide seat 6, the strip-shaped guide groove 21 is matched with the guide slide seat 6, the double-shaft motor 41 is started and drives the first transmission screw rod 42 and the second transmission screw rod 43 to rotate simultaneously, the first transmission screw rod 42 and the second transmission screw rod 43 can drive the first translation sliding block 44 and the second translation sliding block 45 to move oppositely or reversely under the guide action of the guide slide seat 6 in the rotating process, the first translation sliding block 44 and the second translation sliding block 45 can move oppositely or reversely so that the first clamping fixed inserting block 46 and the second clamping fixed inserting block 47 can move oppositely along with the first translation sliding block 44 and the second translation sliding block 45, the second clamping fixed inserting block 47 is arranged at the upper end of the second translation sliding block 45 penetrating out of the rotary table 2, and the first clamping fixed inserting block 46 and the second clamping fixed inserting block 47 are inserted into the positioning seat 31.
Referring to fig. 4 to 8, the crushing mechanism 5 includes a driving motor 51, a driving gear 52, a driven gear 53, a first pulley 54, a second pulley 55, a first belt 56, a first rotary crushing frame 57, a supporting shaft 58, a third pulley 59, a fourth pulley 510, a second belt 511, and a second rotary crushing frame 512, the driving motor 51 is installed inside the centrifugal box 3, an output shaft of the driving motor 51 is spline-mounted with the driving gear 52 and the first pulley 54, the first pulley 54 is connected with the second pulley 55 through the first belt 56, the second pulley 55 is spline-mounted on the first rotary crushing frame 57, the first rotary crushing frame 57 is mounted on one end of the centrifugal box 3, the driving gear 52 is engaged with the driven gear 53, the driven gear 53 is spline-mounted on the supporting shaft 58, the supporting shaft 58 is spline-mounted with the third pulley 59, the third belt pulley 59 is connected to the fourth belt pulley 510 via the second belt 511, the fourth belt pulley 510 is spline-mounted on the second rotary crushing frame 512, the second rotary crushing frame 512 is mounted on the other end of the centrifugal box 3, the bacterial strain cells for producing nitrilase are put into the crushing bin 32, the driving motor 51 drives the driving gear 52 and the first belt pulley 54 to rotate clockwise, the first belt pulley 54 rotates clockwise to rotate the first belt 56, the second belt pulley 55 and the first rotary crushing frame 57 clockwise, at the same time, the driving gear 52 rotates clockwise to rotate the driven gear 53 counterclockwise, the driven gear 53 rotates counterclockwise to rotate the supporting shaft 58, the third belt pulley 59, the second belt 511, the fourth belt pulley 510 and the second rotary crushing frame 512 counterclockwise, the first rotary crushing frame 57 is rotated clockwise in the crushing bin 32 and the second rotary crushing frame 512 is rotated counterclockwise in the crushing bin 32, and the cells of the strain are crushed by the first rotary crushing frame 57 and the second rotary crushing frame 512 which are rotated in opposite directions.
Referring to fig. 9 to 11, the first and second rotary crushing frames 57 and 512 each include a rotary shaft 571, a mounting plate 572, and a rotary crushing roller 573, the rotary shaft 571 is mounted on the second and fourth pulleys 55 and 510, the mounting plate 572 is further mounted on the rotary shaft 571, the rotary crushing roller 573 is mounted on the mounting plate 572, the rotary crushing rollers 573 are annularly distributed on the mounting plate 572, the rotary crushing roller 573 of the first rotary crushing frame 57 is distributed on the periphery of the rotary crushing roller 573 of the second rotary crushing frame 512, the first rotary crushing frame 57 rotates clockwise in the crushing bin 32 and the second rotary crushing frame 512 rotates counterclockwise in the crushing bin 32, and the strain cells are crushed by the first and second rotary crushing frames 57 and 512 having opposite rotation directions.
In order to better demonstrate the safe and environment-friendly process of crushing and centrifuging by using a crushing and centrifuging device for preparing nitrilase, the embodiment now provides a safe and environment-friendly method for crushing and centrifuging by using the crushing and centrifuging device for preparing nitrilase, which comprises the following steps:
s10: the centrifugal box 3 is placed on the rotary table 2, the double-shaft motor 41 is started to drive the first transmission screw rod 42 and the second transmission screw rod 43 to rotate simultaneously, the first transmission screw rod 42 and the second transmission screw rod 43 can drive the first translation sliding block 44 and the second translation sliding block 45 to move oppositely under the guiding action of the guiding sliding seat 6 in the rotating process, the first translation sliding block 44 and the second translation sliding block 45 can move oppositely to enable the first clamping fixed inserting block 46 and the second clamping fixed inserting block 47 to move oppositely along with the first translation sliding block 44 and the second translation sliding block 45 until the first clamping fixed inserting block 46 and the second clamping fixed inserting block 47 are inserted into the positioning seat 31, and at the moment, the centrifugal box 3 is fixed on the rotary table 2;
s20: placing cells of a strain for preparing nitrilase into the crushing bin 32, wherein the driving motor 51 drives the driving gear 52 and the first belt pulley 54 to rotate clockwise, the first belt pulley 54 rotates clockwise to enable the first belt 56, the second belt pulley 55 and the first rotary crushing frame 57 to rotate clockwise, meanwhile, the driving gear 52 rotates clockwise to enable the driven gear 53 to rotate anticlockwise, the driven gear 53 rotates anticlockwise to enable the supporting shaft rod 58, the third belt pulley 59, the second belt 511, the fourth belt pulley 510 and the second rotary crushing frame 512 to rotate anticlockwise, the first rotary crushing frame 57 rotates clockwise in the crushing bin 32 and the second rotary crushing frame 512 rotates anticlockwise in the crushing bin 32, and the cells of the strain are crushed by the first rotary crushing frame 57 and the second rotary crushing frame 512 which are opposite in rotation direction;
s30: after bacterial strain cells are crushed, the embedded driving motor 51 of the base 1 drives the rotary table 2 to rotate at a high speed, the centrifugal box 3 can rotate at a high speed along with the rotary table 2, bacterial strain cells crushed in the crushing bin 32 are subjected to centrifugal operation under the centrifugal force of high-speed rotation, and after bacterial strain cells are subjected to high-speed centrifugation, the crushed and centrifuged suspension for preparing the nitrilase is prepared.
In summary, according to the safe and environment-friendly crushing and centrifuging device and method for preparing nitrilase, the centrifugal box 3 is fixed on the rotary table 2 through the clamping and fixing mechanism 4, the driving motor 51 is started, the first rotary crushing frame 57 rotates clockwise in the crushing bin 32, the second rotary crushing frame 512 rotates anticlockwise in the crushing bin 32, bacterial cells are crushed through the first rotary crushing frame 57 and the second rotary crushing frame 512 which rotate in opposite directions, meanwhile, the rotary table 2 rotates at a high speed to enable the centrifugal box 3 to rotate at a high speed, bacterial cells crushed in the crushing bin 32 are subjected to centrifugal operation under the centrifugal force of high-speed rotation, and after bacterial cells are subjected to high-speed centrifugation, the suspension after crushing and centrifuging for preparing the nitrilase is prepared, so that crushing and centrifuging integration can be realized, the time of crushing and centrifuging is greatly reduced, and the efficiency of crushing and centrifuging is improved.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should be covered by the protection scope of the present invention by making equivalents and modifications to the technical solution and the inventive concept thereof.
Claims (2)
1. The utility model provides a safe and environment-friendly's nitrilase preparation is with broken centrifugal device, includes base (1), a serial communication port, install revolving stage (2) on base (1), be provided with centrifugal box (3) on revolving stage (2), positioning seat (31) are all installed to centrifugal box (3) both sides terminal surface, centrifugal box (3) are through centre gripping fixed establishment of institution (4) centre gripping positioning seat (31) and install on revolving stage (2), be provided with broken storehouse (32) in centrifugal box (3), be provided with broken mechanism (5) in broken storehouse (32), broken mechanism (5) are embedded on centrifugal box (3), the just opposite face of centrifugal box (3) still is provided with observation window (33);
the clamping and fixing mechanism (4) comprises a double-shaft motor (41), a first transmission screw rod (42), a second transmission screw rod (43), a first translation sliding block (44), a second translation sliding block (45), a first clamping and fixing inserting block (46) and a second clamping and fixing inserting block (47), wherein the double-shaft motor (41) is arranged on the inner side of the rotary table (2) through a support, the double shafts of the double-shaft motor (41) are respectively connected with the first transmission screw rod (42) and the second transmission screw rod (43) with opposite screw threads through couplings, one ends of the first transmission screw rod (42) and the second transmission screw rod (43) which are far away from the double-shaft motor (41) are arranged on the inner wall of the rotary table (2) through bearings, the first transmission screw rod (42) is in threaded connection with the first translation sliding block (44), the upper end of the first translation sliding block (44) penetrates out of the rotary table (2) to be provided with the first clamping and fixing inserting block (46), the second transmission screw rod (43) is in threaded connection with the second translation sliding block (45), the upper end of the second translation sliding block (45) is far away from the upper end of the double-shaft motor (41) to be provided with the first clamping and fixing inserting block (47), the first translation sliding block (44) and the second translation sliding block (45) are respectively provided with a guide sliding seat (6), the guide sliding seats (6) are embedded on the inner wall of the rotary table (2), the inner wall of the rotary table (2) is provided with a strip-shaped guide groove (21) for the translation of the guide sliding seats (6), and the strip-shaped guide groove (21) is matched with the guide sliding seats (6);
the crushing mechanism (5) comprises a driving motor (51), a driving gear (52), a driven gear (53), a first belt pulley (54), a second belt pulley (55), a first belt (56), a first rotary crushing frame (57), a supporting shaft rod (58), a third belt pulley (59), a fourth belt pulley (510), a second belt (511) and a second rotary crushing frame (512), wherein the driving motor (51) is arranged on the inner side of the centrifugal box (3), the driving gear (52) and the first belt pulley (54) are arranged on an output shaft of the driving motor (51) through splines, the first belt pulley (54) is connected with the second belt pulley (55) through the first belt pulley (56), the second belt pulley (55) is arranged on the first rotary crushing frame (57) through splines, the first rotary crushing frame (57) is arranged on one end of the centrifugal box (3), the driving gear (52) is meshed with the driven gear (53), the driven gear (53) is arranged on the supporting shaft rod (58) through splines, the supporting shaft rod (58) is further connected with the third belt pulley (59) through the third belt pulley (511), the fourth belt pulley (510) is installed on a second rotary crushing frame (512) through a spline, and the second rotary crushing frame (512) is installed on the other end of the centrifugal box (3);
the first rotary crushing frame (57) and the second rotary crushing frame (512) comprise rotary shafts (571), mounting plates (572) and rotary crushing rollers (573), the rotary shafts (571) are mounted on the second belt pulley (55) and the fourth belt pulley (510), the mounting plates (572) are further mounted on the rotary shafts (571), the rotary crushing rollers (573) are mounted on the mounting plates (572), the rotary crushing rollers (573) are annularly distributed on the mounting plates (572), and the rotary crushing rollers (573) of the first rotary crushing frame (57) are distributed on the periphery of the rotary crushing rollers (573) of the second rotary crushing frame (512).
2. A method of crushing and centrifuging a crushing and centrifuging device for preparing a safe and environment-friendly nitrilase as claimed in claim 1, comprising the steps of:
s10: the centrifugal box (3) is placed on the rotary table (2), the double-shaft motor (41) is started and drives the first transmission screw rod (42) and the second transmission screw rod (43) to rotate simultaneously, the first transmission screw rod (42) and the second transmission screw rod (43) can drive the first translation sliding block (44) and the second translation sliding block (45) to move oppositely under the guiding action of the guiding sliding seat (6), the first translation sliding block (44) and the second translation sliding block (45) can move oppositely so that the first clamping fixed inserting block (46) and the second clamping fixed inserting block (47) can move oppositely along with the first transmission screw rod and the second transmission screw rod until the first clamping fixed inserting block (46) and the second clamping fixed inserting block (47) are inserted into the positioning seat (31), and the centrifugal box (3) is fixed on the rotary table (2) at the moment;
s20: placing bacterial strain cells for preparing nitrilase into a crushing bin (32), wherein a driving motor (51) drives a driving gear (52) and a first belt pulley (54) to rotate clockwise, the first belt pulley (54) rotates clockwise to enable a first belt (56), a second belt pulley (55) and a first rotary crushing frame (57) to rotate clockwise along with the driving gear, meanwhile, the driving gear (52) rotates clockwise to enable a driven gear (53) to rotate anticlockwise, the driven gear (53) rotates anticlockwise to enable a supporting shaft lever (58), a third belt pulley (59), a second belt pulley (511), a fourth belt pulley (510) and a second rotary crushing frame (512) to rotate anticlockwise, the first rotary crushing frame (57) rotates clockwise in the crushing bin (32) and the second rotary crushing frame (512) rotates anticlockwise in the crushing bin (32), and bacterial strain cells are crushed through the first rotary crushing frame (57) and the second rotary crushing frame (512) which are opposite in rotation direction;
s30: after bacterial strain cells are crushed, a driving motor (51) embedded in a base (1) drives a rotary table (2) to rotate at a high speed, and the centrifugal box (3) can rotate at a high speed along with the rotary table (2) by rotating at the high speed, so that bacterial strain cells crushed in a crushing bin (32) are subjected to centrifugal operation under the centrifugal force of high-speed rotation, and after bacterial strain cells are subjected to high-speed centrifugation, a suspension after crushing and centrifugation for preparing nitrilase is prepared.
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| CN110951584A (en) * | 2019-12-04 | 2020-04-03 | 谢双 | Enzyme preparation equipment for pharmaceutical industry |
| CN111632772A (en) * | 2020-04-22 | 2020-09-08 | 贵州医科大学附属医院 | Integrated device for cutting up cartilage tissue and separating and extracting cells |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110951584A (en) * | 2019-12-04 | 2020-04-03 | 谢双 | Enzyme preparation equipment for pharmaceutical industry |
| CN111632772A (en) * | 2020-04-22 | 2020-09-08 | 贵州医科大学附属医院 | Integrated device for cutting up cartilage tissue and separating and extracting cells |
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