CN112495328A - Synthesis method of nucleoside triphosphate competitive inhibitor - Google Patents
Synthesis method of nucleoside triphosphate competitive inhibitor Download PDFInfo
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- CN112495328A CN112495328A CN202011259937.2A CN202011259937A CN112495328A CN 112495328 A CN112495328 A CN 112495328A CN 202011259937 A CN202011259937 A CN 202011259937A CN 112495328 A CN112495328 A CN 112495328A
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
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- B01J19/18—Stationary reactors having moving elements inside
Abstract
The invention belongs to the technical field of nucleoside triphosphates, and particularly relates to a method for synthesizing a nucleoside triphosphate competitive inhibitor. According to the invention, the heating reaction time can be set through the time relay, after the reaction time is over, the time relay can timely close the heating pipe, and simultaneously the refrigerator is started to crystallize the reagent in the reaction tank, so that the time can be conveniently controlled to dissolve and crystallize, and the synthesis quality of the competitive inhibitor is improved.
Description
Technical Field
The invention relates to the technical field of nucleoside triphosphates, in particular to a synthesis method of a nucleoside triphosphate competitive inhibitor.
Background
Nucleoside Triphosphate (NTP), variously called nucleoside triphosphate, is one of several structures of nucleotides, and the molecular structure of the nucleotide has Nucleoside Monophosphate (NMP), Nucleoside Diphosphate (NDP) and Nucleoside Triphosphate (NTP) according to the number of phosphate groups in the nucleotide molecule, the nucleoside is a condensation product of purine or pyrimidine bases and ribose, five hydroxyl groups on the ribose of the condensation product form lipid with triphosphate, and nucleoside triphosphates such as ATP adenosine triphosphate, GTP guanosine triphosphate, CTP cytidine triphosphate and UTP uridine triphosphate are formed, and they are also synthetic precursors of nucleotides corresponding to adenine nucleotides (AMP), guanosine nucleotides (guanylic acid, GMP), cytosine nucleotides (cytidine, CMP), uracil nucleotides (uridylic acid, UMP), Nucleoside Triphosphate (NTP) is a nucleotide containing three phosphate groups, common forms in nature include Adenosine Triphosphate (ATP), Guanosine Triphosphate (GTP), Cytidine Triphosphate (CTP), Thymidine Triphosphate (TTP), and Uridine Triphosphate (UTP), which contain a ribose, and when ribose is substituted for deoxyribose, the nucleoside triphosphate becomes deoxynucleoside triphosphate.
Competitive inhibitors are inhibitors that produce competitive inhibition, which generally have structural similarity to the substrate of the enzyme being inhibited and compete with the substrate for binding sites on the enzyme molecule, thereby producing reversible inhibition of enzyme activity, while another class of competitive inhibitors have no similarity to the substrate in terms of chemical structure and molecular shape, and therefore do not bind to the enzyme at the active center, but bind to sites other than the active center, and the current nucleoside triphosphate competitive inhibitor synthesis method generally comprises heating, stirring, and cooling crystallization in a reaction kettle, thereby crystallizing the nucleoside triphosphate competitive inhibitor into an adult, and the current inhibitor synthesis method requires manual control of reaction time during inhibitor synthesis, has poor control effect, and cannot achieve automatic synthesis control of the inhibitor, resulting in poor inhibitor synthesis quality, to this end, we propose a method for the synthesis of competitive inhibitors of nucleoside triphosphates.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a synthesis method of nucleoside triphosphate competitive inhibitor, which solves the problems that the synthesis quality of the inhibitor is poor due to the fact that the reaction time needs to be controlled manually when the inhibitor is synthesized in the existing inhibitor synthesis method, the control effect is poor, and the automatic synthesis control of the inhibitor cannot be realized.
In order to achieve the purpose, the invention provides the following technical scheme: a synthetic method of nucleoside triphosphate competitive inhibitor comprises a cooling tank, wherein a time relay is fixedly connected to the front of the cooling tank, refrigerators are fixedly connected to the left side surface and the right side surface of the cooling tank, a reaction tank is fixedly connected to the inside of the cooling tank, the top end of the reaction tank penetrates through the cooling tank and extends to the outside of the cooling tank, a case is fixedly connected to the upper surface of the reaction tank, a feeding pipe is fixedly communicated with the left side surface of the reaction tank, a valve is fixedly communicated with the top end of the feeding pipe, a feeding hopper is fixedly communicated with the top end of the valve, a refrigerating end of each refrigerator is fixedly communicated with a refrigerating pipe, each refrigerating pipe is wound on the outer surface of the cooling tank, a temperature sensor is fixedly connected to the front of the reaction tank, a discharge pipe is fixedly communicated with the bottom end of the reaction tank, the bottom end of the discharge pipe penetrates through the cooling tank and extends to the lower side, the bottom mounting intercommunication of delivery pipe has the solenoid valve, the last fixed surface of retort inlays and has the bearing, the inside fixedly connected with rotating electrical machines of quick-witted case, the output fixedly connected with pivot of rotating electrical machines, the bottom of pivot is run through the bearing and is extended to the inside of retort, the puddler that the surface fixedly connected with equidistance of pivot was arranged, the interior diapire fixedly connected with heating pipe of retort.
The reaction tank is characterized in that the right side face of the reaction tank is fixedly communicated with a pressure relief pipe, the top end of the pressure relief pipe is fixedly communicated with a pressure relief box, the inside of the pressure relief box is fixedly connected with a fixing plate, a pressure relief opening is formed in the upper surface of the fixing plate, symmetrical sliding holes are formed in the upper surface of the fixing plate, a pneumatic plate is arranged above the fixing plate, symmetrical sliding rods are fixedly connected to the bottom surface of the pneumatic plate, each sliding rod penetrates through the bottom end of each sliding rod and extends to the lower portion of the fixing plate, each sliding rod is fixedly connected with a reset spring at the bottom end of each sliding rod, and each reset spring is fixedly connected with the inner bottom wall of the pressure relief box at.
The invention discloses a method for synthesizing a nucleoside triphosphate competitive inhibitor, which comprises the following steps:
s1, preparing for work;
s2, adding triphosphate;
s3, adding competitive inhibitor;
s4, heating to dissolve;
s5, cooling and crystallizing;
and S6, packaging the finished product.
As a preferred technical scheme of the invention, the front surface of the case is fixedly hinged with a case door through a hinge, and the front surface of the case door is provided with a heat radiation window.
As a preferred technical scheme of the invention, the front surface of the box door is fixedly connected with a handle, and the outer surface of the handle is sleeved with an anti-skidding sleeve.
As a preferable technical scheme of the invention, a sealing cover is arranged above the feed hopper, and the upper surface of the sealing cover is fixedly connected with a lifting button.
As a preferred technical scheme of the invention, a liquid level window is fixedly embedded in the front of the feed hopper, and scale marks are arranged on the front of the liquid level window.
As a preferable technical scheme of the present invention, a dust screen is disposed above the pressure relief box, and a bottom surface of the dust screen is fixedly connected to an upper surface of the pressure relief box.
As a preferable technical scheme of the invention, the front surface of the reaction tank is fixedly connected with a display screen, and the temperature sensor is electrically connected with the display screen through a lead.
As a preferable technical scheme of the invention, two groups of symmetrical supporting legs are arranged below the cooling tank, and the top end of each supporting leg is fixedly connected with the bottom surface of the cooling tank.
As a preferred embodiment of the present invention, the S1 preparation step, the synthesis equipment and tools are prepared, nucleoside triphosphate and competitive inhibitor are placed in a synthesis material preparation box, the feeding ratio, reaction temperature, reaction time, feeding sequence and manner are defined, the S2 is added with nucleoside triphosphate, the prepared nucleoside triphosphate is weighed in a certain amount in a certain ratio and then added to a reaction apparatus, the temperature change of the reaction apparatus is observed, the S3 is added with competitive inhibitor, the competitive inhibitor is weighed in the same manner and then added with competitive inhibitor, and the competitive inhibitor is added while stirring is required while adding the competitive inhibitor, so that a large amount of competitive inhibitor cannot be added simultaneously.
As a preferred technical scheme of the present invention, the S4 is heated to dissolve, the reaction apparatus is started to heat the added solution, so that the two solvents are quickly dissolved in the reaction apparatus, the solution is continuously stirred during the solution process to accelerate the dissolution rate of the solvent, the S5 is cooled to crystallize, after the two solvents are completely dissolved, the cooling system of the reaction apparatus is started to quickly cool the reaction apparatus, so that the synthesized reagent is crystallized, the S6 finished product is packaged, the crystallized reagent is taken out, and a special reagent bottle with good sealing performance is used for storage.
Compared with the prior art, the invention provides a synthesis method of nucleoside triphosphate competitive inhibitor, which has the following beneficial effects:
1. this synthetic method of nucleoside triphosphate competitive inhibitor through setting up valve and feeder hopper, can add the inside of retort with synthetic reagent, openly sets up the liquid level window through the feeder hopper, can conveniently observe the volume of adding the solution, sets up sealed lid through the top of feeder hopper, can protect the inside solvent of feeder hopper, and sealed lid can avoid retort gaseous emission simultaneously.
2. According to the synthesis method of the nucleoside triphosphate competitive inhibitor, the refrigerators are arranged on two sides of the cooling tank, and the refrigerators are matched with the refrigerating pipes, so that the refrigerating pipes wound on the surface of the reaction tank can rapidly cool the reaction tank, and the reaction reagent is convenient to crystallize.
3. This synthetic method of nucleoside triphosphate competitive inhibitor, the inside heating pipe that sets up through the retort, can heat reactant, guarantee the inside reaction temperature of retort, through time relay, can set up heating reaction time, after reaction time, time relay can in time close the heating pipe, start the refrigerator simultaneously and crystallize the reagent in the retort, be convenient for control time dissolve and crystallize, improve the synthetic quality of competitive inhibitor, drive pivot and puddler through the rotating electrical machines, can stir when adding the solvent, improve the dissolving speed.
4. According to the synthesis method of the nucleoside triphosphate competitive inhibitor, the reaction tank is communicated with the pressure relief box through the pressure relief pipe, when the internal air pressure of the reaction tank is large, the air pressure pushes the pneumatic plate to move through the pressure relief opening, the stability of the movement of the pneumatic plate can be ensured by the cooperation of the sliding hole and the sliding rod, and the pneumatic plate can be reset after pressure relief under the cooperation of the reset spring.
Drawings
FIG. 1 is a front view of a cooling tank of the present invention;
FIG. 2 is a front cross-sectional view of the cooling tank of the present invention;
FIG. 3 is a front cross-sectional view of a reaction tank of the present invention;
FIG. 4 is a front cross-sectional view of the pressure relief tank of the present invention;
FIG. 5 is a flow chart of the synthesis method of the present invention.
In the figure: 1. a cooling tank; 2. a display screen; 3. a refrigerator; 4. supporting legs; 5. a reaction tank; 6. a pressure relief pipe; 7. a pressure relief tank; 8. a dust screen; 9. a handle; 10. a heat dissipation window; 11. a box door; 12. a chassis; 13. a feed hopper; 14. a sealing cover; 15. a liquid level window; 16. a valve; 17. a feed pipe; 18. a time relay; 19. a temperature sensor; 20. a discharge pipe; 21. an electromagnetic valve; 22. a refrigeration pipe; 23. a rotating electric machine; 24. a bearing; 25. a rotating shaft; 26. a stirring rod; 27. heating a tube; 28. a pneumatic plate; 29. a slide hole; 30. a slide bar; 31. a return spring; 32. a fixing plate; 33. a pressure relief port.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
Referring to fig. 1-5, in this embodiment: a method for synthesizing nucleoside triphosphate competitive inhibitor comprises a cooling tank 1, a time relay 18 is fixedly connected to the front surface of the cooling tank 1, the time relay 18 is an automatic switch device for realizing time delay control by utilizing an electromagnetic principle or a mechanical principle, refrigerators 3 are fixedly connected to the left side surface and the right side surface of the cooling tank 1, the refrigerators 3 are the most common refrigeration equipment, two different metals are connected by a conductor, when direct current is switched on, the temperature of one joint is reduced, the temperature of the other joint is increased, a reaction tank 5 is fixedly connected to the inside of the cooling tank 1, the top end of the reaction tank 5 penetrates through the cooling tank 1 and extends to the outside of the cooling tank 1, a case 12 is fixedly connected to the upper surface of the reaction tank 5, a feeding pipe 17 is fixedly communicated to the left side surface of the reaction tank 5, a valve 16 is fixedly communicated to the top end of the feeding pipe 17, and a feeding hopper 13 is, the refrigeration end of every refrigerator 3 all is fixed the intercommunication has refrigeration pipe 22, every refrigeration pipe 22 all twines in the surface of cooling tank 1, the front fixedly connected with temperature sensor 19 of retort 5, the bottom mounting intercommunication of retort 5 has delivery pipe 20, the bottom of delivery pipe 20 runs through cooling tank 1 and extends to the below of cooling tank 1, the bottom mounting intercommunication of delivery pipe 20 has solenoid valve 21, the last fixed surface of retort 5 inlays and has bearing 24, the inside fixedly connected with rotating electrical machines 23 of machine case 12, the output fixedly connected with pivot 25 of rotating electrical machines 23, the bottom of pivot 25 runs through bearing 24 and extends to the inside of retort 5, the outer fixed surface of pivot 25 is connected with the puddler 26 that the equidistance was arranged, the inner diapire fixedly connected with heating pipe 27 of retort 5.
The fixed intercommunication of the right flank of retort 5 has pressure release pipe 6, the fixed intercommunication in top of pressure release pipe 6 has pressure release case 7, the inside fixedly connected with fixed plate 32 of pressure release case 7, pressure release mouth 33 has been seted up to the upper surface of fixed plate 32, symmetrical slide opening 29 has been seted up to the upper surface of fixed plate 32, the top of fixed plate 32 is equipped with pneumatic plate 28, the symmetrical slide bar 30 of bottom surface fixedly connected with of pneumatic plate 28, slide opening 29 and the below of extending to fixed plate 32 are all run through to the bottom of every slide bar 30, the equal fixedly connected with reset spring 31 in bottom of every slide bar 30, the bottom of every reset spring 31 all with the inner diapire fixed connection of pressure release case 7.
The invention discloses a method for synthesizing a nucleoside triphosphate competitive inhibitor, which comprises the following steps: s1 ready for work; s2 adding triphosphate; s3 adding competitive inhibitor; s4 heating to dissolve; s5, cooling and crystallizing; and S6 packaging the finished product.
In this embodiment, case 12's front has box door 11 through the fixed hinge joint of hinge, heat dissipation window 10 has been seted up in box door 11's front, can make things convenient for equipment to dispel the heat, guarantee the normal operating of equipment, box door 11's positive fixedly connected with handle 9, handle 9's surface cover is equipped with the antiskid cover, can conveniently open box door 11, feeder hopper 13's top is equipped with sealed lid 14, the last fixed surface of sealed lid 14 is connected with the lifting button, can play the effect of the inside medicament of protection feeder hopper 13, feeder hopper 13's front fixed inlay has liquid level window 15, liquid level window 15's front is equipped with the scale mark, can be convenient for observe the use amount of.
The top of pressure release case 7 is equipped with dust screen 8, the bottom surface of dust screen 8 is connected with the last fixed surface of pressure release case 7, can play dirt-proof effect, retort 5's positive fixedly connected with display screen 2, temperature sensor 19 passes through the wire and is connected with 2 electricity of display screen, the temperature of retort 5 can be responded to in real time, the below of cooling tank 1 is equipped with two sets of symmetrical supporting legs 4, the top of every supporting leg 4 all with the bottom surface fixed connection of cooling tank 1, the strutting arrangement of can being convenient for.
S1 preparation work, preparation of synthetic equipment and tools, placing nucleoside triphosphate and competitive inhibitor in a synthetic material preparation box, defining the feeding proportion, reaction temperature, reaction time, feeding sequence and mode, S2 adding triphosphate, weighing a certain amount of prepared triphosphate in proportion, then adding into a reaction device, observing the temperature change of the reaction device, S3 adding the competitive inhibitor, weighing the competitive inhibitor in proportion in the same mode, then adding the competitive inhibitor, and adding the competitive inhibitor while stirring while adding the competitive inhibitor, wherein a large amount of competitive inhibitor cannot be added simultaneously.
S4 heating and dissolving, starting the reaction device to heat the added solution, enabling the two solvents to be quickly dissolved in the reaction device, continuously stirring the solution in the solution process, accelerating the dissolution rate of the solvents, cooling and crystallizing S5, starting the cooling system of the reaction device after the two solvents are completely dissolved, quickly cooling the reaction device, crystallizing out the synthesized reagent, packaging the S6 finished product, taking out the crystallized reagent, and storing the reagent by adopting a special reagent bottle with good sealing property.
In this embodiment, the display screen 2, the refrigerator 3, the time relay 18, the temperature sensor 19, the electromagnetic valve 21, the rotating electrical machine 23, and the heating pipe 27 are known technologies that have been widely used in daily life, and have been expanded in the embodiment of the present invention, and are not described herein again.
The working principle and the using process of the invention are as follows: when in use, the device is firstly placed in a laboratory through a supporting leg 4, the device is powered on, before the synthesis of a competitive inhibitor, basic reagents required by the synthesis are prepared properly, a sealing cover 14 is opened, nucleoside triphosphate is placed in a reaction tank 5 through a feed hopper 13 in cooperation with a feed pipe 17, then the competitive inhibitor is added, a valve 16 is controlled to enable the competitive inhibitor to be slowly added into the reaction tank 5, a rotating motor 23 is utilized to drive a rotating shaft 25 and a stirring rod 26 to stir reaction solution, the dissolution of the reaction solvent is accelerated, a time relay 18 is operated, the time relay 18 controls a heating pipe 27 and a refrigerator 3 according to set time to perform antipyretic dissolution and cooling crystallization, the reaction tank 5 can be decompressed through a decompression pipe 6 and a decompression box 7 in the reaction process, when the pressure is too high, an air pressure pushes a pneumatic plate 28 to rise under the cooperation of a sliding rod 30 and a sliding hole 29, accordingly, the pressure is released through the relief port 33, and the pneumatic plate 28 can be released from the pressure by the engagement of the return spring 31 and then returned.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A method for the synthesis of competitive inhibitors of nucleoside triphosphates, comprising a cooling tank (1), characterized in that: the positive fixedly connected with time relay (18) of cooling tank (1), the equal fixedly connected with refrigerator (3) of left and right sides face of cooling tank (1), the inside fixedly connected with retort (5) of cooling tank (1), the top of retort (5) runs through cooling tank (1) and extends to the outside of cooling tank (1), the last fixed surface of retort (5) is connected with quick-witted case (12), the left surface fixed intercommunication of retort (5) has inlet pipe (17), the fixed intercommunication in top of inlet pipe (17) has valve (16), the fixed intercommunication in top of valve (16) has feeder hopper (13), the refrigeration end of every refrigerator (3) all fixed intercommunication has refrigeration pipe (22), every refrigeration pipe (22) all twines in the surface of cooling tank (1), the positive fixedly connected with temperature sensor (19) of retort (5), the bottom end of the reaction tank (5) is fixedly communicated with a discharge pipe (20), the bottom end of the discharge pipe (20) penetrates through the cooling tank (1) and extends to the lower portion of the cooling tank (1), the bottom end of the discharge pipe (20) is fixedly communicated with an electromagnetic valve (21), a bearing (24) is fixedly embedded on the upper surface of the reaction tank (5), a rotating motor (23) is fixedly connected to the inside of the case (12), a rotating shaft (25) is fixedly connected to the output end of the rotating motor (23), the bottom end of the rotating shaft (25) penetrates through the bearing (24) and extends to the inside of the reaction tank (5), stirring rods (26) which are arranged at equal intervals are fixedly connected to the outer surface of the rotating shaft (25), and a heating pipe (27) is fixedly connected to the inner bottom wall of the reaction tank (5);
the reaction tank is characterized in that a pressure relief pipe (6) is fixedly communicated with the right side surface of the reaction tank (5), a pressure relief box (7) is fixedly communicated with the top end of the pressure relief pipe (6), a fixed plate (32) is fixedly connected inside the pressure relief box (7), a pressure relief opening (33) is formed in the upper surface of the fixed plate (32), symmetrical sliding holes (29) are formed in the upper surface of the fixed plate (32), a pneumatic plate (28) is arranged above the fixed plate (32), symmetrical sliding rods (30) are fixedly connected to the bottom surface of the pneumatic plate (28), the bottom end of each sliding rod (30) penetrates through the sliding hole (29) and extends to the lower side of the fixed plate (32), a return spring (31) is fixedly connected to the bottom end of each sliding rod (30), and the bottom end of each return spring (31) is fixedly connected with the inner bottom wall of the pressure relief box (7);
the invention discloses a method for synthesizing a nucleoside triphosphate competitive inhibitor, which comprises the following steps:
s1, preparing for work;
s2, adding triphosphate;
s3, adding competitive inhibitor;
s4, heating to dissolve;
s5, cooling and crystallizing;
and S6, packaging the finished product.
2. The method of synthesizing a competitive nucleoside triphosphate inhibitor of claim 1, wherein: the front of the case (12) is fixedly hinged with a case door (11) through a hinge, and the front of the case door (11) is provided with a heat radiation window (10).
3. The method of synthesizing a competitive nucleoside triphosphate inhibitor of claim 2, wherein: the front of the box door (11) is fixedly connected with a handle (9), and an anti-slip sleeve is sleeved on the outer surface of the handle (9).
4. The method of synthesizing a competitive nucleoside triphosphate inhibitor of claim 1, wherein: a sealing cover (14) is arranged above the feed hopper (13), and a lifting button is fixedly connected to the upper surface of the sealing cover (14).
5. The method of synthesizing a competitive nucleoside triphosphate inhibitor of claim 1, wherein: the front of feeder hopper (13) is fixed to be inlayed and is had liquid level window (15), the front of liquid level window (15) is equipped with the scale mark.
6. The method of synthesizing a competitive nucleoside triphosphate inhibitor of claim 1, wherein: the dust screen (8) is arranged above the pressure relief box (7), and the bottom surface of the dust screen (8) is fixedly connected with the upper surface of the pressure relief box (7).
7. The method of synthesizing a competitive nucleoside triphosphate inhibitor of claim 1, wherein: the front face of the reaction tank (5) is fixedly connected with a display screen (2), and the temperature sensor (19) is electrically connected with the display screen (2) through a lead.
8. The method of synthesizing a competitive nucleoside triphosphate inhibitor of claim 1, wherein: the lower part of the cooling tank (1) is provided with two sets of symmetrical supporting legs (4), and each supporting leg (4) is fixedly connected with the bottom surface of the cooling tank (1) at the top end.
9. The method of synthesizing a competitive nucleoside triphosphate inhibitor of claim 1, wherein: the preparation work of S1, the preparation of synthetic equipment and tools, the placement of nucleoside triphosphates and competitive inhibitors in synthetic material preparation boxes, the material feeding proportion, reaction temperature, reaction time, feeding sequence and mode are defined, the S2 is added with nucleoside triphosphates, a certain amount of prepared nucleoside triphosphates is weighed in proportion, then added into a reaction device, the temperature change of the reaction device is observed, the competitive inhibitors are added in S3, the competitive inhibitors are weighed in the same mode in proportion, then the competitive inhibitors are placed, the competitive inhibitors are placed while stirring, and a large amount of competitive inhibitors cannot be placed simultaneously.
10. The method of synthesizing a competitive nucleoside triphosphate inhibitor of claim 1, wherein: s4 heats and dissolves, starts the reaction device and heats the added solution, so that two solvents are quickly dissolved in the reaction device, and continuously stirs the solution in the solution process, thereby accelerating the dissolution rate of the solvents, S5 cools and crystallizes, and after two solvents are completely dissolved, starts the cooling system of the reaction device, quickly cools the reaction device, thereby crystallizing out the synthesized reagent, packaging the S6 finished product, taking out the crystallized reagent, and storing the reagent by adopting a special reagent bottle with good sealing property.
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