CN112481118A - Sulfate reducing bacteria culture device using straw as carbon source - Google Patents
Sulfate reducing bacteria culture device using straw as carbon source Download PDFInfo
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- CN112481118A CN112481118A CN202011408610.7A CN202011408610A CN112481118A CN 112481118 A CN112481118 A CN 112481118A CN 202011408610 A CN202011408610 A CN 202011408610A CN 112481118 A CN112481118 A CN 112481118A
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- reducing bacteria
- carbon source
- vertical columns
- poling
- heat insulation
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- 241000894006 Bacteria Species 0.000 title claims abstract description 23
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 title claims abstract description 18
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 16
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 16
- 239000010902 straw Substances 0.000 title claims abstract description 16
- 238000009413 insulation Methods 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 238000004321 preservation Methods 0.000 claims description 12
- 238000012258 culturing Methods 0.000 claims 5
- 229910021653 sulphate ion Inorganic materials 0.000 claims 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 abstract 1
- 239000003245 coal Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 238000005485 electric heating Methods 0.000 description 5
- 230000000149 penetrating effect Effects 0.000 description 5
- 238000005065 mining Methods 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a sulfate reducing bacteria culture device taking straws as a carbon source, which comprises a base positioned at the bottom, wherein four vertical columns are arranged above the base and are distributed in a rectangular array, a top plate is arranged above the four vertical columns, power slide blocks are sleeved outside the four vertical columns, racks are embedded on the excircle of each vertical column, the vertical columns penetrate through vertical holes in the power slide blocks, the power slide blocks are fixed with a lower box, the lower box is in a rectangular box-shaped structure with an upward opening, a lower heat insulation layer and a middle heat insulation layer are arranged inside the lower box, the outline of the lower heat insulation layer is in a rectangular plate shape, the outline of the middle heat insulation layer is in a rectangular tube shape, the upper parts of the four vertical columns are inserted into the top plate and are fixed with the top plate, and a lower cover is arranged below the top plate. This sulfate reducing bacteria culture apparatus with straw as carbon source can provide stable cultivation environment for sulfate reducing bacteria culture, is fit for generally using widely.
Description
Technical Field
The invention belongs to the technical field of sulfate reducing bacteria, and particularly relates to a sulfate reducing bacteria culture device taking straws as a carbon source.
Background
Coal as the most important energy variety occupies about 70 percent of the proportion in the primary energy consumption structure of China all the time, coal enterprises make important contributions to the continuous high growth of macroscopic economy for 30 years of the reform and the development of China, but coal resources also generate a large amount of coal mine wastewater in the processes of development, processing, transportation and utilization. At present, due to the restriction of extensive economic development mode in China, about 30 hundred million cubic meters of acidic wastewater can be generated every year, and the utilization rate is less than 30 percent and far lower than the standard of over 80 percent in developed countries such as Europe, America and the like. A large amount of untreated acid mine water is directly discharged, so that the storage water body is seriously polluted, the fragile ecological environment of a mining area is further deteriorated, meanwhile, huge waste of water resources is caused, the current situation of severe water shortage in China is undoubtedly aggravated, and the method is more contradictory to the development concept of clean production and circular economy in China. Therefore, the method has important strategic significance for treating the acidic wastewater of the coal mine, realizing harmless standard-reaching discharge and recycling, protecting the ecological environment of a mining area, relieving the contradiction between energy development and water resource shortage of the mining area and promoting the sustainable development of coal mine enterprises.
In the treatment scheme of acid wastewater treatment in coal mines, sulfate reducing bacteria are used for purification, which is a relatively effective wastewater treatment mode, but the sulfate reducing bacteria need a constant temperature environment in the culture process, most of the existing culture modes place the sulfate reducing bacteria in a culture tank, and the environmental variables in the culture process are difficult to control accurately.
Disclosure of Invention
The invention aims to provide a sulfate reducing bacteria culture device using straws as a carbon source, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a sulfate reducing bacteria culture device with straws as a carbon source comprises a base located at the bottom, wherein four vertical columns are vertically arranged above the base and distributed in a rectangular array, a top plate is arranged above the four vertical columns, power sliders are sleeved outside the four vertical columns, racks are embedded on the outer circles of the vertical columns, the vertical columns penetrate through vertical holes in the power sliders, the power sliders are fixed with a lower box, the lower box is of a rectangular box-shaped structure with an upward opening, a lower heat insulation layer and a middle heat insulation layer are arranged inside the lower box, the outline of the lower heat insulation layer is in a rectangular plate shape, and the outline of the middle heat insulation layer is in a rectangular tube shape;
four the stand top inserts the roof inside and is fixed with the roof, the roof below is equipped with the lower cover, the lower cover below is equipped with the heat preservation, the profile of going up the heat preservation corresponds with the upper end inner space profile of nowel, roof, last heat preservation and lower cover interpolation are equipped with two poling, the poling upper end and lower extreme have all been twisted the nut, and the nut of upper end presses the upper surface of roof, and the nut of lower extreme presses the lower surface at last heat preservation, poling upper end and lower extreme inside all stopper are equipped with sealed the pad.
Preferably, power slider inner space upside is equipped with the motor, the cover is established and is fixed with on the output shaft of motor, with the rack interlock of inlaying the dress on the stand excircle, power slider inner space downside is equipped with the contrary piece, the power slider outside is equipped with the cylinder, the output shaft of cylinder is articulated with the contrary piece that ends, the cover is equipped with the spring on the output shaft of the cylinder in the power slider, the rack interlock on the contrary piece and the stand excircle that ends.
Preferably, a lower pipe is arranged below the penetrating pipe, two ends of the lower pipe are bent and extend towards the penetrating pipe, connecting rings are arranged at two ends of the lower pipe, the connecting rings are in threaded connection with the penetrating pipe and tightly connect the end portions of the lower pipe and the penetrating pipe, radiating fins distributed at equal intervals are arranged on the outer side of the lower pipe, and a fan is arranged above the radiating fins.
Preferably, an upper pipe is arranged above the top plate, connecting rings are arranged at two ends of the upper pipe, the end portion of the upper pipe is tightly connected with the end portion of the penetrating pipe through the connecting rings, the upper pipe is located in the inner space of the upper box, the upper box is fixed above the top plate, and a water pump is arranged on the upper pipe in the upper box.
Preferably, the side wall of the lower side of the upper box is provided with two horizontal positioning pipes, the upper box is internally provided with a pipe frame, an electric heating pipe is inserted into the positioning pipes, and one end of the electric heating pipe, which is back to the positioning pipes, is fixed with the pipe frame.
Preferably, an upper cover covers the opening above the upper box, and a pressure gauge, a thermometer and a pressure release valve are in threaded connection with the upper cover.
The invention has the technical effects and advantages that: according to the sulfate reducing bacteria culture device with the straws as the carbon source, the interior of the upper box is preheated, the heat-conducting medium with stable temperature is conveyed into the lower pipe in the lower box after the temperature in the upper box is controlled, the temperature in the lower box is changed through the radiating fins outside the lower pipe, and compared with the mode of directly heating by using a heating pipe, the temperature in the inner space of the lower box can be stably controlled; the power slide block is used for moving the lower box up and down, the non-return block pushed by the spring can avoid the problem that the lower box accidentally drops, the safety is improved, the motor drives the worm to lift the lower box, and the lower box is stably lifted; the lower heat-insulating layer, the middle heat-insulating layer and the upper heat-insulating layer in the lower box are favorable for keeping the temperature of the inner space of the lower box, and have an auxiliary sealing effect.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the power slider structure of the present invention;
FIG. 3 is a schematic view of the structure at A in FIG. 1 according to the present invention.
In the figure: 1. a base; 2. a column; 201. a rack; 3. a top plate; 4. a power slider; 401. a worm; 402. a motor; 403. a non-return block; 404. a spring; 405. a cylinder; 5. discharging the box; 6. a lower insulating layer; 7. a middle heat-insulating layer; 8. an upper heat-insulating layer; 9. a lower cover; 10. a heat sink; 11. a lower pipe; 12. a connecting ring; 13. pipe penetration; 14. a gasket; 15. a nut; 16. feeding a pipe; 17. feeding the box; 18. a water pump; 19. a positioning tube; 20. an electric heating tube; 21. a pipe frame; 22. an upper cover; 23. a pressure gauge; 24. a thermometer; 25. and (4) releasing the valve.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a sulfate reducing bacteria culture device taking straws as a carbon source, which comprises a base 1 positioned at the bottom, wherein four vertical columns 2 are arranged above the base 1, the four vertical columns 2 are distributed in a rectangular array, a top plate 3 is arranged above the four vertical columns 2, power sliders 4 are sleeved outside the four vertical columns 2, racks 201 are embedded on the outer circles of the vertical columns 2, the vertical columns 2 penetrate through vertical holes in the power sliders 4, the power sliders 4 are fixed with a lower box 5, the lower box 5 is of a rectangular box-shaped structure with an upward opening, a lower heat-insulating layer 6 and a middle heat-insulating layer 7 are arranged inside the lower box 5, the outline of the lower heat-insulating layer 6 is in a rectangular plate shape, and the outline of the middle heat-insulating layer 7 is in a rectangular tube shape;
four 2 tops of stand insert the roof 3 inside and fixed with roof 3, roof 3 below is equipped with lower cover 9, lower cover 9 below is equipped with heat preservation 8, the profile of going up heat preservation 8 corresponds with the upper end inner space profile of nowel 5, roof 3, last heat preservation 8 and lower cover 9 interpolation are equipped with two poling 13, poling 13 upper end and lower extreme have all been twisted nut 15, and the nut 15 of upper end presses the upper surface of roof 3, and the nut 15 of lower extreme presses the lower surface at last heat preservation 8, poling 13 upper end and the inside sealed 14 that all fills up of lower extreme.
Specifically, 4 inner space upside of power slider is equipped with motor 402, the cover is established and is fixed with 401 on the output shaft of motor 402, 401 and 2 excircle on the rack 201 interlock of inlaying the dress, 4 inner space downside of power slider are equipped with and end contrary piece 403, 4 outsides of power slider are equipped with cylinder 405, the output shaft and the end contrary piece 403 of cylinder 405 are articulated, the cover is equipped with spring 404 on the output shaft of the cylinder 405 in the power slider 4, end contrary piece 403 and 2 excircle on the rack 201 interlock of stand, power slider 4 is used for driving the nowel 5 and reciprocates.
Specifically, a lower pipe 11 is arranged below the through pipe 13, two ends of the lower pipe 11 are bent and extend towards the through pipe 13, two ends of the lower pipe 11 are respectively provided with a connecting ring 12, the connecting rings 12 are in threaded connection with the through pipe 13 and tightly connect the end parts of the lower pipe 11 and the through pipe 13, radiating fins 10 distributed at equal intervals are arranged on the outer side of the lower pipe 11, a fan is arranged above the radiating fins 10, and the through pipe 13 connects the top plate 3, the upper heat-insulating layer 8 and the lower cover 9 through nuts 15.
Specifically, top plate 3 top is equipped with upper tube 16, the both ends of upper tube 16 are equipped with go-between 12, the tip of upper tube 16 is tight through the tip of go-between 12 with poling 13, upper tube 16 is located the inner space of upper box 17, upper box 17 is fixed in top plate 3, upper tube 16 in upper box 17 installs water pump 18, and low tube 11, poling 13 and upper tube 16 are inside to be filled with heat-conducting medium.
Specifically, be equipped with two horizontally registration arm 19 on the lateral wall of last case 17 downside, go up the inside pipe support 21 that is equipped with of case 17, electrothermal tube 20 has been inserted to registration arm 19 inside, electrothermal tube 20 one end and pipe support 21 fixed of registration arm 19 dorsad, the inside water that is filled of case 17, electrothermal tube 20 heats the inside water of case 17.
Specifically, it has upper cover 22 to go up the opening of case 17 top and cover, threaded connection has manometer 23, thermometer 24 and relief valve 25 above upper cover 22, and manometer 23, thermometer 24 are used for the inside pressure of control upper box 17 also the temperature, and the inside transshipping of case 17 is avoided to relief valve 25, and upper box 17 and upper cover 22 are fixed through the buckle.
The working principle of the sulfate reducing bacteria culture device with straws as a carbon source is that a cylinder 405 contracts to pull a check block 403 back, a motor 402 in a power slide block 4 rotates to put down a lower box 5, then air supply of the cylinder 405 is stopped, a spring 404 pushes the check block 403 back, a container containing carbonate reducing bacteria is placed in an inner space of the lower box 5, then the lower box 5 is lifted up through the power slide block 4, the check block 403 is sequentially clamped into a rack 201 on the excircle of a stand column 2 in the lifting process, personnel injury caused by accidental falling of the lower box 5 is avoided, an electric heating pipe 20 heats water in an upper box 17, a heat conducting medium in an upper pipe 16 is heated by the water, the heat conducting medium is conveyed through a water pump 18, the heated heat conducting medium is input into a lower pipe 11, heat is dissipated into the inner space of the lower box 5 through the assistance of a fan and a radiating fin 10, the air temperature in the lower box 5 is adjusted to be suitable for sulfate reducing bacteria culture, the internal space of the regulating device 5 has two ways, one is to control the working flow rate of the water pump 18, and the other is to regulate the load of the electric heating tube 20.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (6)
1. The utility model provides an use sulphate reducing bacteria culture apparatus of straw as carbon source, includes base (1) that is located the bottom, its characterized in that: the heat insulation structure is characterized in that four vertical columns (2) are vertically arranged above the base (1), the four vertical columns (2) are distributed in a rectangular array, a top plate (3) is arranged above the four vertical columns (2), a power slide block (4) is sleeved outside the four vertical columns (2), a rack (201) is embedded on the outer circle of each vertical column (2), the vertical columns (2) penetrate through vertical holes in the power slide block (4), the power slide block (4) is fixed with a lower box (5), the lower box (5) is of a rectangular box-shaped structure with an upward opening, a lower heat insulation layer (6) and a middle heat insulation layer (7) are arranged inside the lower box (5), the outline of the lower heat insulation layer (6) is in a rectangular plate shape, and the outline of the middle heat insulation layer (7) is in a rectangular tube shape;
four stand (2) top is inserted roof (3) inside and is fixed with roof (3), roof (3) below is equipped with lower cover (9), lower cover (9) below is equipped with heat preservation (8), the profile of going up heat preservation (8) corresponds with the upper end inner space profile of nowel (5), roof (3), go up heat preservation (8) and lower cover (9) interpolation and be equipped with two poling pipes (13), poling pipe (13) upper end and lower extreme have all been twisted nut (15), and nut (15) of upper end press the upper surface of roof (3), and nut (15) of lower extreme press the lower surface of last heat preservation (8), poling pipe (13) upper end and lower extreme inside all fill in and are equipped with sealed pad (14).
2. The device for culturing sulfate-reducing bacteria by using straws as a carbon source as claimed in claim 1, wherein: the utility model discloses a power slider (4) inner space, including power slider (4), power slider (4) inner space upside is equipped with motor (402), the cover is established and is fixed with (401) on the output shaft of motor (402), rack (201) the interlock of inlaying the dress on (401) and stand (2) excircle, power slider (4) inner space downside is equipped with contrary piece (403), power slider (4) outside is equipped with cylinder (405), the output shaft of cylinder (405) is articulated with contrary piece (403), the cover is equipped with spring (404) on the output shaft of cylinder (405) in power slider (4), it interlocks with rack (201) on stand (2) excircle to end contrary piece (403).
3. The device for culturing sulfate-reducing bacteria by using straws as a carbon source as claimed in claim 1, wherein: the utility model discloses a fan, including poling (13), both ends are bent and are extended to poling (13) down (11), the both ends of downthehole (11) all are equipped with go-between (12), go-between (12) and poling (13) threaded connection and the tip of tightly low tube (11) and poling (13), the low tube (11) outside is equipped with fin (10) that the equidistance distributes, fin (10) top is equipped with the fan.
4. The device for culturing sulfate-reducing bacteria by using straws as a carbon source as claimed in claim 1, wherein: roof (3) top is equipped with upper tube (16), the both ends of upper tube (16) are equipped with go-between (12), the tip of upper tube (16) is passed through go-between (12) and the tip of poling (13) and is tight, upper tube (16) are arranged in the inner space of last case (17), it fixes in roof (3) top to go up case (17), water pump (18) are installed in upper tube (16) in last case (17).
5. The device for culturing sulfate reducing bacteria by using straws as a carbon source as claimed in claim 4, wherein: go up and be equipped with two horizontally registration arm (19) on the lateral wall of case (17) downside, go up case (17) inside and be equipped with pipe support (21), registration arm (19) inside is inserted and is equipped with electrothermal tube (20), electrothermal tube (20) are fixed with pipe support (21) the one end of registration arm (19) dorsad.
6. The device for culturing sulfate reducing bacteria by using straws as a carbon source as claimed in claim 4, wherein: the opening of upper box (17) top coats and is stamped upper cover (22), threaded connection has manometer (23), thermometer (24) and relief valve (25) above upper cover (22).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011408610.7A CN112481118A (en) | 2020-12-05 | 2020-12-05 | Sulfate reducing bacteria culture device using straw as carbon source |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011408610.7A CN112481118A (en) | 2020-12-05 | 2020-12-05 | Sulfate reducing bacteria culture device using straw as carbon source |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112481118A true CN112481118A (en) | 2021-03-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011408610.7A Pending CN112481118A (en) | 2020-12-05 | 2020-12-05 | Sulfate reducing bacteria culture device using straw as carbon source |
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| CN (1) | CN112481118A (en) |
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|---|---|---|---|---|
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2020
- 2020-12-05 CN CN202011408610.7A patent/CN112481118A/en active Pending
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