CN112481108A - Amino acid fermentation system and process - Google Patents

Amino acid fermentation system and process Download PDF

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Publication number
CN112481108A
CN112481108A CN201910860717.6A CN201910860717A CN112481108A CN 112481108 A CN112481108 A CN 112481108A CN 201910860717 A CN201910860717 A CN 201910860717A CN 112481108 A CN112481108 A CN 112481108A
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fermentation
tank
micro
fermentation tank
materials
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张志炳
周政
张锋
李磊
孟为民
王宝荣
杨高东
罗华勋
杨国强
田洪舟
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Nanjing Institute of Microinterface Technology Co Ltd
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Nanjing Institute of Microinterface Technology Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20—Degassing; Venting; Bubble traps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds

Abstract

The invention relates to an amino acid fermentation system and a process, which comprises the following steps: fermentation cylinder, knockout drum, at least two micro-interface generators and back flow. Compared with the traditional method, the invention forms micron-sized bubbles by crushing air, and mixes the micron-sized bubbles with fermentation raw materials to form gas-liquid emulsion, so as to increase the interfacial area of gas-liquid two phases and achieve the effect of strengthening mass transfer within a lower preset operating condition range; meanwhile, the micron-sized bubbles can be fully mixed with the fermentation raw materials to form gas-liquid emulsion, and the gas-liquid two-phase full mixing can ensure that the amino acid producing bacteria in the system can fully absorb oxygen in the materials, so that byproducts are prevented from being generated, and the fermentation efficiency of the system is further improved.

Description

Amino acid fermentation system and process
Technical Field
The invention relates to the technical field of bacterial fermentation, in particular to an amino acid fermentation system and an amino acid fermentation process.
Background
Amino acids are important components of living organisms, are very important substances for nutrition, survival and development of living organisms, and play important roles in substance metabolism regulation and information transmission in living organisms. Amino acid is used as human nutrition additive, flavoring agent, feed additive, medicine, pesticide, etc., and has wide application in food industry, agriculture, animal husbandry, human health, health promotion, etc. The increasing demand for amino acids in various end-use markets, including health foods, artificial sweeteners, dietary supplement products and cosmetics, will also drive the benign growth of the amino acid market in the coming years.
With the vigorous development of biotechnology, more and more amino acid products are produced by a biological fermentation method instead of a chemical synthesis method, and the amino acid products produced by the biological fermentation method have incomparable advantages in the aspects of safety, energy consumption and environmental protection. At present, most of amino acids are directly produced by a biological fermentation method, the market demand for the products is increased year by year, and the volume of a fermentation tank for industrial production of the amino acids is gradually enlarged. However, most of the existing fermentation tank systems are manually operated, and with the continuous increase of the scale of the fermentation tank, the workload and the labor intensity of workers are continuously increased, and the labor cost is also continuously increased; on the other hand, the existing fermentation tank system has a long operation period, and the uncertainty of manual operation causes a large amount of working time to be used for production preparation work, so that the effective production time is reduced, and the utilization rate of equipment is low; in addition, in the actual production process, due to misoperation or inaccuracy, the fluctuation of fermentation contamination and culture process parameters (temperature, pH, dissolved oxygen and the like) is large, and serious loss is caused to the industrial production of amino acid fermentation.
Chinese patent publication No.: CN109022270A discloses a fermentation tank system for amino acid production and a working method thereof, belonging to the technical field of amino acid production. The method comprises the steps of tank washing, air sterilization, feeding, inoculation, cultivation and discharging; each of the steps may be run individually or continuously. It can be seen that the system suffers from the following problems:
firstly, the air is broken only by the stirring blades in the device, and the air forms large bubbles after being broken, but the bubbles cannot be fully mixed with the mixed materials due to overlarge volume, so that oxygen is not uniformly absorbed by bacteria, and the fermentation efficiency of the system is reduced.
Secondly, the device is easy to produce by-products under the condition that bacteria are not uniformly contacted with oxygen, so that materials in the system cannot be used, and the energy consumption of the system is increased.
Thirdly, the device uses the stirring leaf to stir bacterium and material, stirs the in-process stirring leaf and can cause the destruction to the bacterium to lead to bacterium quantity reduction in the raw materials, reduce fermentation efficiency.
Disclosure of Invention
Therefore, the invention provides an amino acid fermentation system and process, which are used for solving the problem of low fermentation efficiency caused by the production of byproducts due to incomplete fermentation of amino acid producing bacteria because sterile air cannot be fully mixed with fermentation raw materials in the prior art.
In one aspect, the present invention provides an amino acid fermentation system comprising:
a fermentor for use in fermenting a material with an amino acid producing species, the fermentor comprising: a complete mixed flow biochemical reaction area which is arranged below and used for loading fermentation raw materials and amino acid production bacteria and providing a reaction space for the fermentation of the fermentation raw materials and the amino acid production bacteria, and a plug flow biochemical reaction area which is arranged above and used for conveying fermented materials and separating gas and liquid;
the separation tank is connected with the fermentation tank and is used for separating the materials output by the fermentation tank to generate gaseous strains and fermentation liquor;
at least two micro-interface generators which are respectively arranged at the appointed positions in the fully mixed flow reaction zone, convert the pressure energy of gas and/or the kinetic energy of liquid into the surface energy of bubbles and transmit the surface energy to the sterile air, so that the sterile air is crushed into micron-sized bubbles with the diameter of more than or equal to 1 mu m and less than 1mm to improve the mass transfer area between the fermentation raw material and the sterile air, reduce the thickness of a liquid film and reduce the mass transfer resistance, and the fermentation raw material and the micron-sized bubbles are mixed to form a gas-liquid emulsion after being crushed, so as to strengthen the mass transfer efficiency between the fermentation raw material and the sterile air within the range of preset operation conditions;
and the return pipes are respectively connected with the fermentation tank and the separation tank and used for exchanging heat of the materials output by the fermentation tank and shunting the materials after heat exchange so as to respectively return to the fermentation tank or output to the separation tank.
Further, the micro-interface generator includes:
the first micro-interface generator is a pneumatic micro-interface generator, is arranged in the fully mixed flow biochemical reaction zone and is positioned at the bottom of the reaction zone, and is used for crushing sterile air to form micro-bubbles at a micrometer scale and outputting the micro-bubbles to a fermentation tank after the crushing is finished;
and the second micro-interface generator is a hydrodynamic type or gas-liquid linkage type micro-interface generator, is arranged in the fully-mixed flow biochemical reaction area and positioned at the top of the reaction area and used for receiving the material output by the return pipe, uses the material to entrain the sterile air which is not fully used in the plug flow biochemical reaction area and breaks the sterile air into micro-bubbles with micron scale, and mixes the micro-bubbles with the material to form a gas-liquid emulsion to be output to the fully-mixed flow biochemical reaction area.
Further, the complete mixed flow biochemical reaction area in the fermentation tank comprises:
the grating is arranged inside the fermentation tank and is used for filtering insoluble particles in the material;
the pH adjusting liquid feeding port is arranged on the side wall of the fermentation tank, is positioned above the grating and is used for conveying pH adjusting liquid to adjust the pH value of the material;
the fermentation raw material feeding hole is formed in the side wall of the fermentation tank, is positioned below the grating and is used for conveying fermentation raw materials;
the fermentation strain feeding hole is formed in the side wall of the fermentation tank and located below the fermentation raw material feeding hole, and is used for conveying amino acid production strains to the interior of the fermentation tank and fermenting the fermentation raw materials;
a first gaseous feed conduit disposed in a side wall of the fermentor and connected to the micro-interface generator for lateral delivery of sterile air into the micro-interface generator within the fermentor;
a residue outlet arranged at the bottom of the fermentation tank and used for discharging fermented residue out of the system;
and the partition plate is arranged on the inner wall of the fermentation tank and positioned below the grating and used for blocking the fermentation raw material output by the fermentation raw material inlet and the fluctuation generated by the amino acid producing bacteria output by the fermentation strain feed inlet.
Further, the plug flow reaction zone comprises:
the exhaust pipeline is arranged at the top end of the fermentation tank and used for exhausting tail gas generated in the fermentation of the materials in the fermentation tank;
the second gas-phase feeding pipeline is arranged at the top end of the fermentation tank, is connected with the micro-interface generator and is used for conveying the tail gas generated at the top of the fermentation tank into the micro-interface generator in the fermentation tank;
the discharge port is arranged on the side wall of the fermentation tank and used for outputting the fermented materials out of the fermentation tank;
and the backflow feeding pipeline is arranged on the side wall of the fermentation tank and used for outputting part of materials output by the backflow pipe to the micro-interface generator in the fermentation tank.
Further, the return pipe includes:
the circulating pump is connected with the fermentation tank and used for outputting materials fermented in the fermentation tank;
and the heat exchanger is connected with the circulating pump and used for exchanging heat of the material output by the circulating pump so as to enable the material to reach the specified temperature.
Furthermore, the output end of the heat exchanger is provided with a shunt tube, and the shunt tube is respectively connected with the fermentation tank and the separation tank and used for respectively refluxing the materials and outputting the materials to the separation tank.
Further, the separation tank is a sealed tank, and comprises:
the feeding hole is arranged at the top end of the separation tank and used for conveying the material output by the return pipe to the inside of the separation tank;
the exhaust port is arranged at the top end of the separation tank and used for outputting gaseous strains;
and the discharge port is arranged at the bottom end of the separation tank and used for outputting the separated fermentation liquor and conveying the fermentation liquor to the next working section.
In another aspect, the present invention also provides an amino acid fermentation process, comprising:
step 1: conveying the specified type of fermentation raw materials to a complete mixed flow biochemical reaction area in the fermentation tank through the fermentation raw material feeding hole, and conveying the amino acid producing bacteria to the fermentation tank through the fermentation strain feeding hole;
step 2: conveying sterile air to the first micro-interface generator through the first gas-phase feeding pipeline, crushing the sterile air into micron-sized bubbles by the first micro-interface generator, outputting the micron-sized bubbles to a fermentation raw material in the fermentation tank, and sufficiently mixing the micron-sized bubbles with the fermentation raw material to provide an aerobic environment for the amino acid producing bacteria;
and step 3: the amino acid producing bacteria and the fermentation raw materials react in an aerobic environment, after the fermentation is finished, the fermentation tank conveys the fermented materials to the plug flow biochemical reaction area, the materials flow through the grating, the grating can filter residues in the materials, and the filtered residues can settle to the bottom of the fermentation tank and are discharged out of the fermentation tank through the residue outlet;
and 4, step 4: the filtered materials flow in the plug flow biochemical reaction area along a designated direction, when the materials flow to the top of the fermentation tank, gas in the materials is output to the fermentation tank through an exhaust pipeline, and the materials are output to the return pipe through a first discharge hole;
and 5: the reflux pipe pumps out the materials in the fermentation tank, and carries out shunting after heat exchange, and part of the materials after heat exchange flows back to the second micro-interface generator to adjust the temperature in the fully mixed flow biochemical reaction area, and the other part of the materials is output to the separation tank for separation;
step 6: after heat exchange, the material flows back and enters the second micro-interface generator through a backflow feeding pipeline, the second micro-interface generator receives tail gas at the top of the fermentation tank through the second gas-phase feeding pipeline, the tail gas is crushed into micron-scale bubbles by using the material, the micron-scale bubbles and the material are mixed to form a gas-liquid emulsion, and after the gas-liquid emulsion is formed, the gas-liquid emulsion is output to the fully-mixed flow biochemical reaction area by the second micro-interface generator so as to adjust the temperature in the fully-mixed flow biochemical reaction area while the material is repeatedly used;
and 7: after heat exchange, the material is output to the separation tank, the separation tank can carry out gas-liquid separation on the material to form gaseous strains and fermentation liquor, after separation, the gaseous strains are discharged out of the separation tank through the exhaust port, and the fermentation liquor is discharged out of the separation tank through the second discharge port and conveyed to the next working section.
Further, when the system operates, the pH adjusting liquid feeding port can convey pH adjusting liquid to adjust the pH value of the materials in the fermentation tank.
Compared with the prior art, the method has the beneficial effects that compared with the traditional method, the method has the advantages that micron-scale micron-sized bubbles are formed by crushing air, so that the micron-scale bubbles are mixed with fermentation raw materials to form gas-liquid emulsion, the phase interface area of gas-liquid two phases is increased, and the effect of strengthening mass transfer within a lower preset operation condition range is achieved; meanwhile, the micron-sized bubbles can be fully mixed with the fermentation raw materials to form gas-liquid emulsion, and the gas-liquid two-phase full mixing can ensure that the amino acid producing bacteria in the system can fully absorb oxygen in the materials, so that byproducts are prevented from being generated, and the fermentation efficiency of the system is further improved. Meanwhile, a backflow feeding pipe is arranged in the fermentation tank of the system, and the material after reaction flows back to the fermentation tank, so that the contact time of sterile air and the fermentation raw material is prolonged, and the fermentation efficiency is improved. In addition, the range of the preset operation condition can be flexibly adjusted according to different product requirements or different catalysts, so that the full and effective reaction is further ensured, and the reaction rate is further ensured.
In addition, the range of the preset operation conditions can be flexibly adjusted according to different raw material compositions or different product requirements, so that the full and effective reaction is further ensured, the reaction rate is further ensured, and the purpose of strengthening the reaction is achieved.
Particularly, the full-mixed flow biochemical reaction area is arranged in the fermentation tank, the micro-interface generator is arranged in the full-mixed flow biochemical reaction area, so that the inside of the full-mixed flow biochemical reaction area is closer to a full-mixed flow model, the uniformity of the temperature and the concentration of materials in the reaction area is ensured, and the materials can be quickly and uniformly mixed when entering the reaction area, thereby preventing the generation of byproducts caused by the fact that part of amino acid producing bacteria in the reaction area cannot absorb enough oxygen, and further improving the fermentation efficiency of the system.
Particularly, the invention also arranges a plug flow biochemical reaction area in the fermentation tank, the plug flow biochemical reaction area enables the fermented material to move at a uniform speed along the designated direction, the material is effectively prevented from flowing back in the conveying process, and the plug flow biochemical reaction area can further promote the reaction rate of the material in the fully mixed flow biochemical reaction area, thereby further improving the fermentation efficiency of the system.
Furthermore, the fermentation tank is respectively provided with the pneumatic micro-interface generator and the gas-liquid linkage micro-interface generator, and the micron-sized bubbles and the materials are mixed more uniformly by using different types of micro-interface generators, so that the mixing efficiency of the materials and the sterile air in the fermentation tank is improved, and the fermentation efficiency of the system is further improved.
Furthermore, a grating is arranged in the fermentation tank, and residues in the materials can be effectively filtered and discharged through a residue outlet at the bottom of the fermentation tank, so that the purity of the fermentation liquor is improved.
Particularly, the side wall of the fermentation tank is also provided with a pH adjusting liquid feeding port, when the system operates, the pH adjusting liquid feeding port can adjust the pH value of the material in the fermentation tank in a mode of conveying the pH adjusting liquid into the fermentation tank, and the pH value of the material can be effectively adjusted while amino acid producing bacteria are not damaged, so that the reaction efficiency of the amino acid producing bacteria is improved.
Especially, the fermentation cylinder inner wall still is equipped with the baffle, the baffle is located fermentation raw materials feed inlet with the exit of fermentation bacterial feed inlet is through two the feed inlet export is sheltered from to prevent that each feed inlet from producing undulant when exporting material and amino acid producing fungus and being right the second micro-interface generator causes the influence and reduces the mixing efficiency of second micro-interface generator.
Furthermore, a return pipe is further arranged in the system, and the fermented materials are returned to be reused, so that the utilization rate of the materials is improved, and the fermentation efficiency of the system is further improved.
Particularly, the return pipe is internally provided with a heat exchanger, when the fermented materials are returned and output, the materials can be subjected to heat exchange through the heat exchanger so as to reach the specified temperature, and the temperature of the materials in the fermentation tank is adjusted, so that a proper fermentation environment is provided for amino acid producing bacteria in the fermentation tank, and the fermentation efficiency of the system is further improved.
Drawings
FIG. 1 is a schematic diagram of the amino acid fermentation system according to the present invention.
Detailed Description
Preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only for explaining the technical principle of the present invention, and do not limit the scope of the present invention.
It should be noted that in the description of the present invention, the terms of direction or positional relationship indicated by the terms "upper", "lower", "left", "right", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, which are only for convenience of description, and do not indicate or imply that the device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
Furthermore, it should be noted that, in the description of the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Please refer to fig. 1, which is a schematic structural diagram of an amino acid fermentation system according to the present invention, including a fermentation tank 1, a micro-interface generator 2 (not shown), a return pipe 3 and a separation tank 4. The micro-interface generator 2 is arranged in the fermentation tank and used for crushing sterile air to form micron-sized bubbles and mixing the micron-sized bubbles with materials in the fermentation tank to form a gas-liquid emulsion, so that an aerobic environment is provided for amino acid producing bacteria in the materials. The return pipe 3 is connected with the fermentation tank 1 and used for outputting the fermented materials in the fermentation tank 1 and returning part of the output materials to the fermentation tank 1. The separation tank 4 is connected with an output branch in the return pipe 3 and is used for separating and concentrating the material output by the fermentation tank 1 and outputting the treated fermentation liquor to the next working section.
When the system is operated, firstly, fermentation raw materials and amino acid production bacteria are conveyed into the fermentation tank 1, and simultaneously, sterile air is conveyed into the fermentation tank 1, the sterile air can enter the micro-interface generator 2, the micro-interface generator 2 breaks the sterile air to form micron-scale micro-scale bubbles, and the micron-scale bubbles are mixed with the fermentation raw materials to form gas-liquid emulsion so as to provide uniform aerobic environment for the amino acid production bacteria, after the fermentation is completed, the fermentation tank 1 can respectively discharge gas and residues generated in the fermentation process out of the system, and output the fermented materials to the return pipe 3, the return pipe 3 conducts heat exchange on the materials and then shunts the materials, after shunting, a part of the materials flow back to the fermentation tank 1, when the materials are repeatedly used, the temperature of the materials in the fermentation tank is adjusted, the other part of the materials can be output to the separation tank 4, and the separation tank can conduct gas, discharging residual gaseous bacteria in the materials and outputting the fermentation liquor after separation and concentration to the next working section. It will be understood by those skilled in the art that the fermentation bacteria species used in the system may be amino acid-producing bacteria, or other species, and the present embodiment is not particularly limited as long as the species is capable of aerobic fermentation.
With continued reference to FIG. 1, the fermenter 1 of the present invention includes a mixed flow biochemical reaction area 11 and a plug flow biochemical reaction area 12. Wherein the complete mixed flow biochemical reaction area 11 is positioned at the lower part of the fermentation tank 1 and is used for fully mixing the amino acid producing bacteria and the micron-sized bubbles with the fermentation raw materials. The plug flow biochemical reaction area 12 is located at the upper part of the fermentation tank 1, and is used for conveying fermented materials along a specified direction while promoting the reaction speed in the fermentation tank 1. When the fermentation tank 1 starts to ferment, the mixed flow biochemical reaction area 11 respectively receives the amino acid producing bacteria, the fermentation raw material and the micron-sized bubbles and fully mixes the amino acid producing bacteria, the fermentation raw material and the micron-sized bubbles to ferment the amino acid producing bacteria in an aerobic environment, after the fermentation is finished, the mixed flow biochemical reaction area 11 conveys the fermented material to the plug flow biochemical reaction area 12, and the plug flow biochemical reaction area 12 conveys the material to a specified direction. It is understood that the length-diameter ratio of the plug flow biochemical reaction zone 12 is not particularly limited in this embodiment, as long as the length of the plug flow biochemical reaction zone 12 is sufficient to maintain a continuous and stable flow of the material.
Referring to fig. 1, the mixed flow biochemical reaction area 11 of the present invention includes a grating 111, a pH adjusting liquid inlet 112, a fermentation material inlet 113, a fermentation strain inlet 114, a first gas phase inlet channel 115, a residue outlet 116, and a partition 117. Wherein, the grating 111 is arranged inside the fermentation tank 1 for filtering the residue generated in the fermentation process of the fermentation tank 1. The pH adjusting liquid feeding hole is formed in the inner wall of the fermentation tank 1 and located above the grating 111, and is used for conveying pH adjusting liquid to the fermentation tank 1. The fermentation material inlet 113 is disposed on a sidewall of the fermentation tank 1 and below the grating 111, and is used for delivering fermentation material to the fermentation tank 1. The fermentation strain feed port 114 is arranged on the side wall of the fermentation tank 1 and below the fermentation raw material feed port 113, and is used for conveying amino acid production strains to the fermentation tank 1. The first gas phase feed line 115 is provided in the side wall of the fermenter 1 and connected to the micro-interface generator 2 for supplying sterile air. The residue outlet 116 is disposed at the bottom of the fermentation tank 1 for discharging the residue generated after fermentation out of the fermentation tank 1. The partition 117 is disposed on the inner wall of the fermentation tank 1 and located on the same side as the fermentation material inlet 113, so as to block the fluctuation generated when the fermentation material inlet 113 outputs fermentation material and the fermentation strain inlet 114 outputs amino acid-producing strain.
When the mixed flow biochemical reaction area is operated, the fermentation material inlet 113 can convey the fermentation material to the inside of the fermentation tank 1, the fermentation strain inlet 114 can convey the amino acid producing bacteria to the inside of the fermentation tank 1, the baffle 117 can block the fluctuation generated when the fermentation material inlet 113 and the fermentation strain inlet 114 output the material, the first gas phase inlet pipeline 115 can convey the sterile air to the micro interface generator 2, the micro interface generator 2 breaks the sterile air to form micron-scale bubbles, and the micron-scale bubbles are mixed with the fermentation material to form gas-liquid emulsion, the gas-liquid emulsion is mixed with the amino acid producing bacteria to start fermentation, in the fermentation process, the pH adjusting liquid inlet 113 can convey the pH adjusting liquid to the fermentation tank 1 to adjust the pH value of the mixed material in the fermentation tank 1, after the fermentation is completed, the mixed flow biochemical reaction area 11 can convey the fermented material to the flat plug flow biochemical reaction area 12, during the transportation, the grid 111 will filter out the residues in the material, which after filtering out start to settle and leave the fermentation tank 1 through the residue outlet 116.
Specifically, the grating 111 is a sieve plate, which is disposed inside the fermentation tank 1 for filtering the fermented material. After the complete fermentation of the mixed flow biochemical reaction zone 11, the fermented material will flow through the grating 111, and the grating 111 will filter the residue in the material. It is understood that the kind of the grid 111 and the size of the through holes are not particularly limited in this embodiment, as long as the grid 111 can filter solid phase residues in the material.
In particular, the first gas feed line 115 is arranged in the side wall of the fermenter and the outlet of the first gas feed line 115 is connected to the micro-interfacial generator 2 for feeding sterile air to the micro-interfacial generator 2. When the mixed flow biochemical reaction zone 11 is operated, the first gas phase feeding pipe 115 will convey the sterile air to the micro interface generator 2, the micro interface generator 2 will crush the sterile air to form micron-sized bubbles, and the micron-sized bubbles are output to the interior of the fermentation tank 1 and mixed with the fermentation raw material. It is understood that the material and dimensions of first gas feed conduit 115 are not particularly limited in this embodiment, provided that first gas feed conduit 115 is capable of delivering a specified volume of sterile air over a specified period of time.
Specifically, the partition 117 is a baffle plate fixedly connected to the inner wall of the fermentation tank 1 for blocking the fluctuation of the fermentation tank when receiving the material. When the complete mixed flow biochemical reaction zone 11 is operated, the fermentation raw material inlet 113 can convey fermentation raw materials into the fermentation tank 1, the fermentation strain inlet 114 can convey amino acid producing strains into the fermentation tank 1, and the partition plate 117 can be blocked at the discharging position, so that the fluctuation of the two materials in the conveying process is prevented, and the influence of the fluctuation on the micro-interface generator is prevented. It will be appreciated that the connection of the partition 117 to the fermenter 1 may be by welding, by integral connection or by other types of connection, provided that the partition 117 is able to reach its designated operating condition.
Referring to fig. 1, the plug flow biochemical reaction area 12 of the present invention is located at the upper portion of the fermentation tank 1, and is used for transporting fermented materials along a designated direction, and includes an exhaust pipe 121, a second gas phase feeding pipe 122, a first discharge port 123 and a return feeding pipe 124. Wherein the exhaust pipeline 121 is arranged at the top of the fermentation tank 1 for exhausting gas generated in the fermentation process. The second gas phase feed line 122 is arranged at the top of the fermenter 1 and connected to the micro-interface generator 2 for feeding the fermenter top off-gas. The first discharge port 123 is arranged on the side wall of the fermentation tank 1 and used for outputting fermented materials. The backflow feeding pipe 124 is disposed on the sidewall of the fermentation tank 1 and below the discharge port 123, and is used for feeding the backflow materials to the fermentation tank 1. When the plug flow reaction zone 12 is in operation, the material is conveyed upward at a constant speed in the reaction zone, when the material reaches the top of the fermentation tank 1, the gas in the material is output from the fermentation tank 1 through the exhaust pipe 121, the liquid-phase material is output to the return pipe 3 through the first discharge port 123, and part of the material is returned through the return pipe 3 and then returned to the fermentation tank 1 through the return feed pipe 124, after the return, the second gas-phase feed pipe 122 conveys the tail gas to the micro-interface generator 2, and the tail gas is mixed with the returned material after being crushed and conveyed to the fully mixed flow biochemical reaction zone 11 for reuse.
Specifically, the second gas phase feeding pipe 122 is disposed at the top of the fermentation tank, and the outlet of the second gas phase feeding pipe 122 is connected to the micro-interface generator 2, so as to convey the tail gas from the top of the fermentation tank to the micro-interface generator 2. When the plug flow biochemical reaction region 12 operates, the second gas phase feeding pipe 122 will feed the tail gas to the micro interface generator 2, and the micro interface generator 2 will crush the tail gas to form micro-bubbles, and the micro-bubbles are output to the inside of the fermentation tank 1 and mixed with the material. It is understood that the material and size of the second gas-phase feed pipe 122 are not particularly limited in this embodiment, as long as the second gas-phase feed pipe 122 can deliver a given volume of off-gas in a given time.
Specifically, the backflow feeding pipe 124 is disposed on the sidewall of the fermentation tank 1, and the outlet of the backflow feeding pipe 124 is connected to the micro-interfacial surface generator 2, so as to convey the backflow materials to the micro-interfacial surface generator 2. When the plug flow biochemical reaction zone is in operation, the return pipe 3 will deliver the returned material to the return feed pipe 124, and the return feed pipe 124 will deliver the material to the micro-interface generator 2 to mix the material with the micro-scale bubbles. It is understood that the material and size of the backflow feeding pipe 124 are not limited in this embodiment, as long as the backflow feeding pipe 124 can deliver a given flow rate of material in a given time.
Referring still to fig. 1, the micro-interface generator 2 of the present invention includes a first micro-interface generator 21 and a second micro-interface generator 22. Wherein the first micro-interface generator 21 is disposed at the bottom of the mixed flow biochemical reaction zone 11 for breaking the sterile air to form micro-scale bubbles. The second micro-interface generator 22 is disposed on the top of the mixed flow biochemical reaction region 11 and connected to the grid 111, and is used for breaking the sterile air into micro-bubbles and mixing the micro-bubbles with the backflow material. When the fermentation tank 1 is in operation, the first micro-interface generator 21 can crush the sterile air to form micron-sized bubbles, and mix the micron-sized bubbles with the fermentation raw material to form a gas-liquid emulsion, and the second micro-interface generator 22 receives the returned material and the sterile air respectively, crushes the sterile air into the micron-sized bubbles, and mixes the micron-sized bubbles with the material to form the gas-liquid emulsion. It is understood that the micro-interface generator 2 of the present invention can also be used in other multi-phase reactions, such as multi-phase fluid, micro-nano-scale particle, micro-bubble micro-emulsion, micro-flow, micro-dispersion, two micro-mixed flow, micro-turbulence, micro-bubble flow, micro-bubble, micro-bubble micro-bubble flow, micro-nano-scale bubble flow, micro-bubble flow, micro-nano-scale particle, micro-bubble micro-fluid, micro-bubble micro-bubble flow, micro-bubble micro-, Or multiphase fluid (micro interface fluid for short) formed by micro-nano-scale particles, thereby effectively increasing the phase boundary mass transfer area between the gas phase and/or the liquid phase and/or the solid phase in the reaction process.
Specifically, the first micro-interface generator 21 of the present invention is a pneumatic micro-interface generator, which is connected to the first gas phase feeding pipe 115, and is used for breaking up the air conveyed by the first gas phase feeding pipe 115 and forming micron-sized bubbles. When the fermentation tank 1 is in operation, the first gas phase feeding pipe 115 will feed the sterile air to the first micro-interface generator 21, the first micro-interface generator 21 will break the sterile air and form micron-sized bubbles, and after the breaking, the first micro-interface generator 21 will output the micron-sized bubbles to the inside of the fermentation tank 1 and mix with the fermentation raw material to provide an aerobic environment for the amino acid producing bacteria.
Specifically, the second micro-interface generator 22 of the present invention is a gas-liquid linkage micro-interface generator, which is connected to the second gas-phase feeding pipe 122 and the backflow feeding pipe 124, respectively, for receiving the tail gas and the backflow material, respectively, and crushing the tail gas into micron-sized micro-bubbles through the pressure of the backflow material. When the second micro-interface generator 22 operates, it will receive the tail gas and the backflow material, respectively, and utilize the pressure energy of the backflow material to break the tail gas into micron-sized bubbles, and form a gas-liquid emulsion by mixing the micron-sized bubbles with the backflow material and output the gas-liquid emulsion to the complete mixed flow biochemical reaction zone 11 for repeated fermentation.
With continued reference to fig. 1, the return line 3 according to the invention comprises a circulation pump 31 and a heat exchanger 32. Wherein the circulating pump 31 is connected with the first material outlet 123 for pumping out the fermented material in the fermentation tank 1. The heat exchanger 32 is connected with the circulating pump 31 and used for exchanging heat for the material output by the circulating pump 31. After the fermentation of the materials in the fermentation tank 1 is completed, the circulation pump 31 starts to operate, the materials are pumped out through the first material outlet 123, and the materials are conveyed to the heat exchanger 32, the heat exchanger 32 exchanges heat with the materials and splits the heat exchanged materials, a part of the materials is refluxed to the reflux feeding pipeline 124, and another part of the materials is output to the separation tank 4. It is understood that the type and power of the circulating pump 31 are not particularly limited in this embodiment, as long as the circulating pump 31 can reach its designated operating state.
Specifically, a diversion pipeline is arranged at the outlet of the heat exchanger 32, one end of the diversion pipeline is connected with the backflow feeding pipeline 124 for returning a part of the materials output by the heat exchanger 32, and the other end of the diversion pipeline is connected with the separation tank 4 for outputting another part of the materials output by the heat exchanger 32 to the separation tank 4 for separation.
With reference to fig. 1, the separating tank 4 of the present invention is a sealed tank, and a feeding port is disposed at the top end of the sealed tank, and the feeding port is connected to the heat exchanger 32 for receiving the material after completion of waste heat; the top end of the separation tank 4 is also provided with an exhaust port for exhausting gaseous bacteria during separation; and a second discharge hole is formed in the bottom end of the separation tank 4 and used for outputting the separated fermentation liquor to the next working section. When the heat exchanger 32 outputs the heat-exchanged material, the material enters the separation tank 4 through the feed inlet and is subjected to gas-liquid separation, after separation, the amino acid producing bacteria remaining in the material and the gas are discharged out of the separation tank 4 through the exhaust port, and the material is separated to form fermentation liquor and is output to the next working section through the second discharge port. It is understood that the material and size of the separation tank 4 are not limited in this embodiment, as long as the separation tank 4 can reach its designated working state.
In order that the objects and advantages of the invention will be more clearly understood, the invention is further described below with reference to examples; it should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
An amino acid fermentation process, comprising the following steps:
step 1: conveying the specified type of fermentation raw materials to a complete mixed flow biochemical reaction area in the fermentation tank through the fermentation raw material feeding hole, and conveying the amino acid producing bacteria to the fermentation tank through the fermentation strain feeding hole;
step 2: conveying sterile air to the first micro-interface generator through the first gas-phase feeding pipeline, crushing the sterile air into micron-sized bubbles by the first micro-interface generator, outputting the micron-sized bubbles to a fermentation raw material in the fermentation tank, and sufficiently mixing the micron-sized bubbles with the fermentation raw material to provide an aerobic environment for the amino acid producing bacteria;
and step 3: the amino acid producing bacteria and the fermentation raw materials react in an aerobic environment, after the fermentation is finished, the fermentation tank conveys the fermented materials to the plug flow biochemical reaction area, the materials flow through the grating, the grating can filter residues in the materials, and the filtered residues can settle to the bottom of the fermentation tank and are discharged out of the fermentation tank through the residue outlet;
and 4, step 4: the filtered materials flow in the plug flow biochemical reaction area along a designated direction, when the materials flow to the top of the fermentation tank, gas in the materials is output to the fermentation tank through an exhaust pipeline, and the materials are output to the return pipe through a first discharge hole;
and 5: the reflux pipe pumps out the materials in the fermentation tank, and carries out shunting after heat exchange, and part of the materials after heat exchange flows back to the second micro-interface generator to adjust the temperature in the fully mixed flow biochemical reaction area, and the other part of the materials is output to the separation tank for separation;
step 6: after heat exchange, the material flows back and enters the second micro-interface generator through a backflow feeding pipeline, the second micro-interface generator receives tail gas at the top of the fermentation tank through the second gas-phase feeding pipeline, the tail gas is crushed into micron-scale bubbles by using the material, the micron-scale bubbles and the material are mixed to form a gas-liquid emulsion, and after the gas-liquid emulsion is formed, the gas-liquid emulsion is output to the fully-mixed flow biochemical reaction area by the second micro-interface generator so as to adjust the temperature in the fully-mixed flow biochemical reaction area while the material is repeatedly used;
and 7: after heat exchange, the material is output to the separation tank, the separation tank can carry out gas-liquid separation on the material to form gaseous strains and fermentation liquor, after separation, the gaseous strains are discharged out of the separation tank through the exhaust port, and the fermentation liquor is discharged out of the separation tank through the second discharge port and conveyed to the next working section.
Specifically, the fermentation process comprises an adaptation phase, a logarithmic growth phase, a growth stop phase and a late fermentation phase, wherein in the adaptation phase: controlling the seed amount and fermentation conditions to shorten the period of the adaptation period, wherein the adaptation period lasts for 2-4 h.
At logarithmic growth phase: the temperature in the fermentation unit 4 is maintained at 30-32 ℃, urea is filled in the pH adjusting tank 43, a nitrogen source necessary for the growth of the thalli is timely supplied by adopting a urea feeding method, and the pH in the fermentation tank is maintained at 7.5-8.0.
At the growth arrest phase: the temperature in the fermentation unit 4 is maintained at 34-37 ℃, the pH adjusting tank 43 is filled with urea, and urea is fed in time to provide enough ammonia, and the pH in the fermentation tank is maintained at 7.2-7.4.
And in the later stage of fermentation, detecting the acid concentration in the fermentation tank, and when the acid concentration is not increased after the nutrients are exhausted, putting the fermentation tank in time. The fermentation period is generally 30 h.
Example one
Performing biological fermentation of amino acids using the above system and process, wherein, during the acclimation period: the seed amount and the fermentation condition are controlled to shorten the period of the adaptation period, and the adaptation period lasts for 2 hours.
At logarithmic growth phase: the temperature in the fermentation unit 4 is maintained at 30 ℃, urea is filled in the pH adjusting tank 43, a nitrogen source necessary for the growth of the thalli is timely supplied by adopting a urea feeding method, and the pH in the fermentation tank is maintained at 7.5.
At the growth arrest phase: the temperature in the fermentation unit 4 is maintained at 34 ℃, urea is filled in the pH adjusting tank 43, and urea is fed in time to provide enough ammonia, so that the pH in the fermentation tank is maintained at 7.2.
And in the later stage of fermentation, detecting the acid concentration in the fermentation tank, and when the acid concentration is not increased after the nutrients are exhausted, putting the fermentation tank in time. The fermentation period is generally 30 h.
Through detection, the purity of the amino acid prepared by using the system and the process is 99.53%.
Example two
Performing biological fermentation of amino acids using the above system and process, wherein, during the acclimation period: the seed amount and the fermentation condition are controlled to shorten the period of the adaptation period, and the adaptation period lasts for 4 hours.
At logarithmic growth phase: the temperature in the fermentation unit 4 is maintained at 32 ℃, urea is filled in the pH adjusting tank 43, a nitrogen source necessary for the growth of the thalli is timely supplied by adopting a urea feeding method, and the pH in the fermentation tank is maintained at 8.0.
At the growth arrest phase: the temperature in the fermentation unit 4 is maintained at 37 ℃, urea is filled in the pH adjusting tank 43, and urea is fed in time to provide enough ammonia, so that the pH in the fermentation tank is maintained at 7.4.
And in the later stage of fermentation, detecting the acid concentration in the fermentation tank, and when the acid concentration is not increased after the nutrients are exhausted, putting the fermentation tank in time. The fermentation period is generally 30 h.
Through detection, the purity of the amino acid prepared by using the system and the process is 99.65%.
EXAMPLE III
Performing biological fermentation of amino acids using the above system and process, wherein, during the acclimation period: the seed amount and the fermentation condition are controlled to shorten the period of the adaptation period, and the adaptation period lasts for 3 hours.
At logarithmic growth phase: the temperature in the fermentation unit 4 is maintained at 31 ℃, urea is filled in the pH adjusting tank 43, a nitrogen source necessary for the growth of the thalli is timely supplied by adopting a urea feeding method, and the pH in the fermentation tank is maintained at 7.8.
At the growth arrest phase: the temperature in the fermentation unit 4 is maintained at 36 ℃, urea is filled in the pH adjusting tank 43, and urea is fed in time to provide enough ammonia, so that the pH in the fermentation tank is maintained at 7.3.
And in the later stage of fermentation, detecting the acid concentration in the fermentation tank, and when the acid concentration is not increased after the nutrients are exhausted, putting the fermentation tank in time. The fermentation period is generally 30 h.
Through detection, the purity of the amino acid prepared by using the system and the process is 99.82%.
Comparative example
The biological fermentation of amino acids was carried out using the prior art, wherein the parameters at each stage during the fermentation were the same as in the examples.
Through detection, the purity of the amino acid prepared by using the system and the process is 98.49%.
So far, the technical solutions of the present invention have been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of the present invention is obviously not limited to these specific embodiments. Equivalent changes or substitutions of related technical features can be made by those skilled in the art without departing from the principle of the invention, and the technical scheme after the changes or substitutions can fall into the protection scope of the invention.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention; various modifications and alterations to this invention will become apparent to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (9)

1. An amino acid fermentation system, comprising:
a fermentor for use in fermenting a material with an amino acid producing species, the fermentor comprising: a complete mixed flow biochemical reaction area which is arranged below and used for loading fermentation raw materials and amino acid production bacteria and providing a reaction space for the fermentation of the fermentation raw materials and the amino acid production bacteria, and a plug flow biochemical reaction area which is arranged above and used for conveying fermented materials and separating gas and liquid;
the separation tank is connected with the fermentation tank and is used for separating the materials output by the fermentation tank to generate gaseous strains and fermentation liquor;
at least two micro-interface generators which are respectively arranged at the appointed positions in the fully mixed flow reaction zone, convert the pressure energy of gas and/or the kinetic energy of liquid into the surface energy of bubbles and transmit the surface energy to the sterile air, so that the sterile air is crushed into micron-sized bubbles with the diameter of more than or equal to 1 mu m and less than 1mm to improve the mass transfer area between the fermentation raw material and the sterile air, reduce the thickness of a liquid film and reduce the mass transfer resistance, and the fermentation raw material and the micron-sized bubbles are mixed to form a gas-liquid emulsion after being crushed, so as to strengthen the mass transfer efficiency between the fermentation raw material and the sterile air within the range of preset operation conditions;
and the return pipes are respectively connected with the fermentation tank and the separation tank and used for exchanging heat of the materials output by the fermentation tank and shunting the materials after heat exchange so as to respectively return to the fermentation tank or output to the separation tank.
2. The amino acid fermentation system of claim 1, wherein the micro-interface generator comprises:
the first micro-interface generator is a pneumatic micro-interface generator, is arranged in the fully mixed flow biochemical reaction zone and is positioned at the bottom of the reaction zone, and is used for crushing sterile air to form micro-bubbles at a micrometer scale and outputting the micro-bubbles to a fermentation tank after the crushing is finished;
and the second micro-interface generator is a hydrodynamic type or gas-liquid linkage type micro-interface generator, is arranged in the fully-mixed flow biochemical reaction area and positioned at the top of the reaction area and used for receiving the material output by the return pipe, uses the material to entrain the sterile air which is not fully used in the plug flow biochemical reaction area and breaks the sterile air into micro-bubbles with micron scale, and mixes the micro-bubbles with the material to form a gas-liquid emulsion to be output to the fully-mixed flow biochemical reaction area.
3. The amino acid fermentation system of claim 1, wherein the mixed flow biochemical reaction zone in the fermentation tank comprises:
the grating is arranged inside the fermentation tank and is used for filtering insoluble particles in the material;
the pH adjusting liquid feeding port is arranged on the side wall of the fermentation tank, is positioned above the grating and is used for conveying pH adjusting liquid to adjust the pH value of the material;
the fermentation raw material feeding hole is formed in the side wall of the fermentation tank, is positioned below the grating and is used for conveying fermentation raw materials;
the fermentation strain feeding hole is formed in the side wall of the fermentation tank and located below the fermentation raw material feeding hole, and is used for conveying amino acid production strains to the interior of the fermentation tank and fermenting the fermentation raw materials;
a first gaseous feed conduit disposed in a side wall of the fermentor and connected to the micro-interface generator for lateral delivery of sterile air into the micro-interface generator within the fermentor;
a residue outlet arranged at the bottom of the fermentation tank and used for discharging fermented residue out of the system;
and the partition plate is arranged on the inner wall of the fermentation tank and positioned below the grating and used for blocking the fermentation raw material output by the fermentation raw material inlet and the fluctuation generated by the amino acid producing bacteria output by the fermentation strain feed inlet.
4. The amino acid fermentation system of claim 1, wherein the plug flow reaction zone comprises:
the exhaust pipeline is arranged at the top end of the fermentation tank and used for exhausting tail gas generated in the fermentation of the materials in the fermentation tank;
the second gas-phase feeding pipeline is arranged at the top end of the fermentation tank, is connected with the micro-interface generator and is used for conveying the tail gas generated at the top of the fermentation tank into the micro-interface generator in the fermentation tank;
the discharge port is arranged on the side wall of the fermentation tank and used for outputting the fermented materials out of the fermentation tank;
and the backflow feeding pipeline is arranged on the side wall of the fermentation tank and used for outputting part of materials output by the backflow pipe to the micro-interface generator in the fermentation tank.
5. The amino acid fermentation system of claim 1, wherein the return line comprises:
the circulating pump is connected with the fermentation tank and used for outputting materials fermented in the fermentation tank;
and the heat exchanger is connected with the circulating pump and used for exchanging heat of the material output by the circulating pump so as to enable the material to reach the specified temperature.
6. The amino acid fermentation system according to claim 1, wherein the output end of the heat exchanger is provided with a shunt pipe, and the shunt pipe is respectively connected with the fermentation tank and the separation tank and is used for respectively refluxing the materials and outputting the materials to the separation tank.
7. The amino acid fermentation system of claim 1, wherein the separation tank is a sealed tank comprising:
the feeding hole is arranged at the top end of the separation tank and used for conveying the material output by the return pipe to the inside of the separation tank;
the exhaust port is arranged at the top end of the separation tank and used for outputting gaseous strains;
and the discharge port is arranged at the bottom end of the separation tank and used for outputting the separated fermentation liquor and conveying the fermentation liquor to the next working section.
8. An amino acid fermentation process, comprising:
step 1: conveying the specified type of fermentation raw materials to a complete mixed flow biochemical reaction area in the fermentation tank through the fermentation raw material feeding hole, and conveying the amino acid producing bacteria to the fermentation tank through the fermentation strain feeding hole;
step 2: conveying sterile air to the first micro-interface generator through the first gas-phase feeding pipeline, crushing the sterile air into micron-sized bubbles by the first micro-interface generator, outputting the micron-sized bubbles to a fermentation raw material in the fermentation tank, and sufficiently mixing the micron-sized bubbles with the fermentation raw material to provide an aerobic environment for the amino acid producing bacteria;
and step 3: the amino acid producing bacteria and the fermentation raw materials react in an aerobic environment, after the fermentation is finished, the fermentation tank conveys the fermented materials to the plug flow biochemical reaction area, the materials flow through the grating, the grating can filter residues in the materials, and the filtered residues can settle to the bottom of the fermentation tank and are discharged out of the fermentation tank through the residue outlet;
and 4, step 4: the filtered materials flow in the plug flow biochemical reaction area along a designated direction, when the materials flow to the top of the fermentation tank, gas in the materials is output to the fermentation tank through an exhaust pipeline, and the materials are output to the return pipe through a first discharge hole;
and 5: the reflux pipe pumps out the materials in the fermentation tank, and carries out shunting after heat exchange, and part of the materials after heat exchange flows back to the second micro-interface generator to adjust the temperature in the fully mixed flow biochemical reaction area, and the other part of the materials is output to the separation tank for separation;
step 6: after heat exchange, the material flows back and enters the second micro-interface generator through a backflow feeding pipeline, the second micro-interface generator receives tail gas at the top of the fermentation tank through the second gas-phase feeding pipeline, the tail gas is crushed into micron-scale bubbles by using the material, the micron-scale bubbles and the material are mixed to form a gas-liquid emulsion, and after the gas-liquid emulsion is formed, the gas-liquid emulsion is output to the fully-mixed flow biochemical reaction area by the second micro-interface generator so as to adjust the temperature in the fully-mixed flow biochemical reaction area while the material is repeatedly used;
and 7: after heat exchange, the material is output to the separation tank, the separation tank can carry out gas-liquid separation on the material to form gaseous strains and fermentation liquor, after separation, the gaseous strains are discharged out of the separation tank through the exhaust port, and the fermentation liquor is discharged out of the separation tank through the second discharge port and conveyed to the next working section.
9. The amino acid fermentation process of claim 8, wherein the pH adjustment liquid feed port delivers a pH adjustment liquid for adjusting the pH of the contents of the fermentor during operation of the system.
CN201910860717.6A 2019-09-11 2019-09-11 Amino acid fermentation system and process Withdrawn CN112481108A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113583822A (en) * 2021-07-14 2021-11-02 南京延长反应技术研究院有限公司 Device and method for strengthening biological fermentation of atmospheric gas source micro-interface
CN113684115A (en) * 2021-08-31 2021-11-23 南京延长反应技术研究院有限公司 Micro-interface mass transfer enhanced fermentation system and method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113583822A (en) * 2021-07-14 2021-11-02 南京延长反应技术研究院有限公司 Device and method for strengthening biological fermentation of atmospheric gas source micro-interface
WO2023284025A1 (en) * 2021-07-14 2023-01-19 南京延长反应技术研究院有限公司 Apparatus and method for enhancing biological fermentation by using atmospheric air source micro-interface
CN113684115A (en) * 2021-08-31 2021-11-23 南京延长反应技术研究院有限公司 Micro-interface mass transfer enhanced fermentation system and method

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Application publication date: 20210312