CN112480458B - High-enzyme-activity modified membrane and preparation method thereof - Google Patents
High-enzyme-activity modified membrane and preparation method thereof Download PDFInfo
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- C08J2379/00—Characterised by the use of macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing nitrogen with or without oxygen, or carbon only, not provided for in groups C08J2361/00 - C08J2377/00
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Abstract
The invention belongs to the field of modified membrane preparation, and particularly relates to a high-enzyme-activity modified membrane and a preparation method thereof. The preparation method comprises the following steps: diluting the pepsin powder with a citric acid-sodium hydroxide-hydrochloric acid buffer solution to obtain a pepsin solution, refrigerating the pepsin solution, and treating the pepsin solution with a pulse electric field to obtain a high-enzyme-activity enzyme solution; soaking a PES (polyether sulfone) membrane in a Tris-HCl buffer solution, adding procyanidine and polyethyleneimine to obtain a mixed solution, stirring until the procyanidine and the polyethyleneimine are coated, and cleaning to obtain the modified membrane; and immersing the modified membrane in the high-enzyme-activity enzyme solution to obtain the high-enzyme-activity modified membrane. The modified membrane with continuous high enzyme activity prepared by the method has high enzyme activity, and simultaneously solves the problems of repeated use capability of enzyme, hydrophilicity and pollution resistance of the membrane.
Description
Technical Field
The invention belongs to the field of preparation of modified membranes, and particularly relates to a high-enzyme-activity modified membrane and a preparation method thereof.
Background
High-voltage pulsed electric fields, as an emerging non-thermal physical sterilization technology, have been used to modulate the activity and stability of many enzymes due to their advantages of low energy consumption, short treatment time, and no chemical residue, with great success, indicating that low-intensity high-voltage pulsed electric fields can enhance enzyme activity. However, the enzyme activity after the high-voltage pulse electric field treatment is reduced in a period of time and is even lower than that of the untreated enzyme, so that the repeated use of the enzyme is greatly reduced.
The enzyme is used as a biocatalyst, is sensitive to environment and is volatile, and not only can the enzyme be reused, but also the stability of the enzyme is increased by immobilizing the enzyme. However, the structure of the enzyme is changed after the enzyme is immobilized, and the flexibility and the activity of the enzyme are greatly reduced. Meanwhile, these physical immobilization methods have poor stability and are prone to enzyme leakage and loss during the reaction process.
Disclosure of Invention
The invention aims to provide a high-enzyme-activity modified membrane and a preparation method thereof, aiming at the problems of reduction of enzyme activity after enzyme immobilization and enzyme loading capacity of enzyme on the membrane.
In order to achieve the above object, a first aspect of the present invention provides a method for preparing a high enzyme activity modified membrane, the method comprising:
(1) Preparation of enzyme solution with high enzyme activity
Diluting the pepsin powder with a citric acid-sodium hydroxide-hydrochloric acid buffer solution to obtain a pepsin solution, refrigerating the pepsin solution, and treating the pepsin solution with a pulse electric field to obtain a high-enzyme-activity enzyme solution;
(2) Preparation of modified membranes
Soaking a PES membrane in a Tris-HCl buffer solution, adding procyanidine and polyethyleneimine to obtain a mixed solution, stirring until the procyanidine and the polyethyleneimine are coated, and then washing with deionized water to obtain the modified membrane;
(3) Preparation of modified membrane with high enzyme activity
Immersing the modified membrane in the high-enzyme-activity enzyme solution, optionally stirring, and washing by using a citric acid-sodium hydroxide-hydrochloric acid buffer solution to obtain the high-enzyme-activity modified membrane.
The modified membrane with continuous high enzyme activity produced by the method provided by the invention has high enzyme activity, and improves the reuse capacity of the enzyme and the service life of the membrane.
Preferably, the citric acid-sodium hydroxide-hydrochloric acid buffer has a pH =3.0 and a concentration of 0.2M.
Preferably, the Tris-HCl buffer has a pH =8.5.
Preferably, the temperature for refrigeration is 3-5 deg.C, such as 4 deg.C.
Preferably, in step (1), the pulsed electric field treatment conditions include: the electric field intensity is 4-16 kV/cm, the flow rate is 40-100 mL/min -1 (ii) a As a further preferable mode, in the step (1), the conditions of the pulsed electric field treatment include: the electric field intensity is 8-11 kV/cm, the flow rate is 70-90 mL/min -1 . The electric field strength is more preferably 8 to 11kV/cm, and the most preferable value is 10kV/cm.
Preferably, in the step (2), the concentration is 10-15cm 2 The PES membrane of (1), wherein the addition amount of procyanidin is 0-1 g and does not include 0, and the addition amount of polyethyleneimine is 0-1 g and does not include 0; as a further preferable mode, in the step (2), the concentration is set to 10 to 15cm 2 The PES membrane of (1), the adding amount of procyanidin is 0.1-0.3g, the adding amount of polyethyleneimine is 0.2-0.4g,the most preferred embodiment is: relative to 10-15cm 2 The amount of procyanidin added to PES membrane (0.2 g) and the amount of polyethyleneimine added to 0.3g, for example, relative to 13.4cm 2 The PES membrane (b) was prepared such that the amount of proanthocyanidin added was 0.2g and the amount of polyethyleneimine added was 0.3g.
Preferably, in the step (2), the stirring time is 8-10h.
Preferably, in step (3), the stirring time is 0 to 6 hours excluding 0, more preferably 1 to 4 hours, and most preferably 1.5 to 3 hours.
Preferably, in step (3), the concentration of pepsin in the high-enzyme-activity enzyme solution is 1 to 6mg/mL, more preferably 3.5 to 5mg/mL.
The second aspect of the present invention provides a high enzyme activity modified membrane obtained by the above-described production method.
The invention has the beneficial effects that:
procyanidins are a class of polyphenolic compounds that are widely found in plants and in everyday foods. In the present invention, since procyanidin has a phenolic hydroxyl group in its structure, it can be used as a surface coating material by mixing it with polyethyleneimine because it can react with amino-rich substances by shiff base reaction and michael addition reaction. The membrane is subjected to procyanidine-polyethyleneimine codeposition, so that the surface of the membrane is rich in amino, the hydrophilicity and the pollution resistance of the membrane are improved, the service life of the membrane is prolonged, and the cleaning cost is reduced.
The modified membrane with continuous high enzyme activity prepared by the method has high enzyme activity, and simultaneously solves the problems of repeated use capability of enzyme, hydrophilicity and pollution resistance of the membrane.
Additional features and advantages of the invention will be set forth in the detailed description which follows.
Drawings
FIG. 1 shows the results of the pepsin activity tests of preparation examples 2 to 9.
Fig. 2 shows results of the pepsin activity tests of preparation example 1, preparation example 10 to preparation example 15.
Fig. 3 shows the results of infrared spectroscopy on Polyethersulfone (PES) raw membranes and the modified membrane prepared in step (1) of example 1.
Figure 4 shows scanning electron micrographs of polyethersulfone protomembrane.
Fig. 5 shows scanning electron micrographs of procyanidins and polyethyleneimine after modification.
Fig. 6 shows the contact angle detection results of a Polyethersulfone (PES) raw membrane and a modified membrane prepared in step (1) of example 1.
Fig. 7 shows the results of the stability test of the high enzyme activity modified membrane prepared in step (2) of example 1.
FIG. 8 shows the results of detection of pepsin activity on the surface of the modified membrane with high enzyme activity under enzyme solutions with different concentrations of high enzyme activity.
Fig. 9 shows the results of pepsin activity detection on the surface of the high-enzyme-activity modified membrane at different stirring times.
Fig. 10 is a schematic flow chart illustrating a method for preparing a high-enzyme-activity modified membrane according to an embodiment of the present invention.
Detailed Description
Preferred embodiments of the present invention will be described in more detail below. While the following describes preferred embodiments of the present invention, it should be understood that the present invention may be embodied in various forms and should not be limited by the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.
Test example 1
The method for measuring the activity of the pepsin comprises the following steps: 20mL of 0.2mmol/L solution (NaH) was added to a 20mL test tube 2 PO 4 -citric acid buffer pH = 3.0), then 0.1mL each enzyme solution prepared in preparation example was added, the mixture was reacted at 37 ℃ for 37min, 10% trichloroacetic acid was added, shaken well, centrifuged at 10000rpm for 15min, absorbance was measured at 280nm wavelength, and enzyme activity was calculated. Definition of enzyme activity unit: the amount of enzyme required to hydrolyze BSA at pH =3.0 and 37 ℃ for 1min to release 1 μmol tyrosine was 1 enzyme activity unit (U).
Preparation example 1
This preparation example prepared a high enzyme activity enzyme solution. The method comprises the following steps:
diluting pepsin powder with 0.2M citric acid-sodium hydroxide-hydrochloric acid buffer solution with pH =3.0 to obtain pepsin solution, refrigerating at 4 deg.C, and treating with pulsed electric field with electric field strength of 10kV/cm and flow rate of 80mL min -1 Obtaining high-enzyme-activity enzyme liquid, wherein the concentration of pepsin in the high-enzyme-activity enzyme liquid is 6mg/mL, and the enzyme activity of the pepsin in the high-enzyme-activity enzyme liquid is 50 (10) 3 ，U)。
Preparation example 2
The difference from preparation example 1 was that the electric field strength was 0kV/cm, and the flow rate was 0 mL-min -1 The enzyme activity is 30 (10) 3 ，U)。
Preparation example 3
The difference from preparation example 1 was that the electric field strength was 4kV/cm, and the flow rate was 50 mL-min -1 The enzyme activity is 35.5 (10) 3 ，U)。
Preparation example 4
The difference from preparation example 1 was that the electric field strength was 6kV/cm, and the flow rate was 50 mL-min -1 The enzyme activity is 36 (10) 3 ，U)。
Preparation example 5
The difference from preparation example 1 was that the electric field strength was 8kV/cm, and the flow rate was 50 mL-min -1 Enzyme activity of 37 (10) 3 ，U)。
Preparation example 6
The difference from preparation example 1 was that the electric field strength was 10kV/cm, and the flow rate was 50 mL-min -1 The enzyme activity is 37.5 (10) 3 ，U)。
Preparation example 7
The difference from preparation example 1 was that the electric field strength was 12kV/cm, and the flow rate was 50 mL-min -1 The enzyme activity is 35.5 (10) 3 ，U)。
Preparation example 8
The difference from preparation example 1 was that the electric field strength was 14kV/cm, and the flow rate was 50 mL-min -1 The enzyme activity is 35.5 (10) 3 ，U)。
Preparation example 9
And preparation ofExample 1 is different in that the electric field strength was 16kV/cm and the flow rate was 50 mL-min -1 The enzyme activity is 32.5 (10) 3 ，U)。
The results of the pepsin activity tests of preparation examples 2 to 9 are shown in fig. 1.
Preparation example 10
The difference from preparation example 1 is that the flow rate is 40 mL/min -1 。
Preparation example 11
The difference from preparation example 1 is that the flow rate was 50 mL. Min -1 。
Preparation example 12
The difference from preparation example 1 is that the flow rate was 60 mL. Min -1 。
Preparation example 13
The difference from preparation example 1 is that the flow rate was 70 mL. Min -1 。
Preparation example 14
The difference from preparation example 1 is that the flow rate was 90 mL. Min -1 。
Preparation example 15
The difference from preparation example 1 is that the flow rate was 100 mL. Min -1 。
The results of the pepsin activity tests of preparation 1, preparation 10 to preparation 15 are shown in fig. 2.
Example 1
This example provides a high enzyme activity modified membrane. The method comprises the following steps:
(1) The diameter of the tube is 13.4cm 2 Soaking the PES membrane in 100mL of Tris-HCl buffer solution with the pH =8.5, adding 0.2g of procyanidine and 0.3g of polyethyleneimine to obtain a mixed solution, stirring at room temperature for 9 hours until the procyanidine and the polyethyleneimine are coated, and then washing with deionized water for three times to obtain the modified membrane.
(2) The modified membrane was immersed in 20mL of the high-enzyme-activity enzyme solution prepared in preparation example 1, stirred slightly at room temperature for 2 hours, and washed three times with a 0.2M-citric acid-sodium hydroxide-hydrochloric acid buffer solution having a pH =3.0 to obtain a high-enzyme-activity modified membrane.
Example 2
The difference from example 1 is that in step (2), the concentrations of the high-enzyme-activity enzyme solutions were 1mg/mL, 2mg/mL, 3mg/mL, 4mg/mL, 5mg/mL, and 6mg/mL, respectively, and the results of the high-enzyme-activity modified pepsin activity on the membrane surface are shown in fig. 8.
Example 3
The difference from example 1 is that in step (2), the stirring time is 1h, 2h, 3h, 4h and 5h, and the result of the pepsin activity on the surface of the modified membrane with high enzyme activity is shown in fig. 9.
Test example 2
The modified film prepared in step (1) of example 1 was examined by an infrared spectrometer, and the infrared spectrum is shown in fig. 3, which shows that: compared with a Polyethersulfone (PES) raw membrane, the membrane after the co-deposition of the procyanidine and the polyethyleneimine is 3100cm -1 ～3600cm -1 An absorption peak appears at the position, which is that-OH functional groups are introduced into a polyether sulfone protomembrane, and infrared and electronic scanning electron microscope results show that procyanidine and polyethyleneimine are successfully introduced into the polyether sulfone membrane.
Test example 3
The modified membrane prepared in the step (1) of example 1 is observed by a scanning electron microscope, as shown in fig. 4 and 5, fig. 4 is a scanning electron microscope image of a polyethersulfone protomembrane, fig. 5 is a scanning electron microscope image of modified proanthocyanidin and polyethyleneimine, and as can be seen from fig. 4 and 5, the modified membrane prepared in the experiment has a rough and compact surface and greatly increases enzyme fixing points.
Test example 4
The modified film prepared in step (1) of example 1 was measured using a contact angle, and the contact angle graph is shown in fig. 6, from which fig. 6 shows that: compared with a polyether sulfone (PES) original membrane, the membrane contact angle after the co-deposition of the procyanidine and the polyethyleneimine is obviously reduced, and the combination of infrared and contact angle results shows that the modified membrane has good hydrophilicity and pollution resistance.
Test example 5
The stability test is performed on the high-enzyme-activity modified membrane prepared in the step (2) in the example 1, and the test result is shown in fig. 7, which shows that the enzyme in the high-enzyme-activity modified membrane has good reuse capacity.
Fig. 10 shows a schematic flow diagram of a method of making a high enzyme activity modified membrane, according to an embodiment. The method comprises the following steps: treating the pepsin with a pulse electric field to obtain a high-enzyme-activity pepsin enzyme solution; co-precipitating and coating a polyether sulfone ultrafiltration membrane (PES) with procyanidine and polyethyleneimine to obtain a modified membrane; and immersing the modified membrane in the high-enzyme-activity pepsin enzyme solution to obtain the high-enzyme-activity modified membrane.
Having described embodiments of the present invention, the foregoing description is intended to be exemplary, not exhaustive, and not limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments.
Claims (10)
1. A preparation method of a high-enzyme-activity modified membrane is characterized by comprising the following steps:
(1) Preparation of enzyme solution with high enzyme activity
Diluting the pepsin powder with a citric acid-sodium hydroxide-hydrochloric acid buffer solution to obtain a pepsin solution, refrigerating the pepsin solution, and treating the pepsin solution with a pulse electric field to obtain a high-enzyme-activity enzyme solution;
(2) Preparation of modified membranes
Soaking a PES membrane in a Tris-HCl buffer solution, adding procyanidine and polyethyleneimine to obtain a mixed solution, stirring until the procyanidine and the polyethyleneimine are coated, and then washing with deionized water to obtain the modified membrane;
the Tris-HCl buffer pH =8.5;
(3) Preparation of modified membrane with high enzyme activity
Immersing the modified membrane in the high-enzyme-activity enzyme solution, optionally stirring, and washing by using a citric acid-sodium hydroxide-hydrochloric acid buffer solution to obtain the high-enzyme-activity modified membrane.
2. The production method according to claim 1,
the citric acid-sodium hydroxide-hydrochloric acid buffer solution has a pH =3.0 and a concentration of 0.2M;
the refrigerating temperature is 3-5 ℃.
3. The production method according to claim 1, wherein in the step (1), the conditions of the pulsed electric field treatment include:
the electric field intensity is 4-16 kV/cm, the flow rate is 40-100 mL/min -1 。
4. The production method according to claim 3, wherein in the step (1), the conditions of the pulsed electric field treatment include:
the electric field intensity is 8-11 kV/cm, the flow rate is 70-90 mL/min -1 。
5. The production method according to claim 1, wherein, in the step (2),
relative to 10-15cm 2 The PES membrane of (1) may contain 0 to 1g of procyanidin, not 0, and 0 to 1g of polyethyleneimine, not 0.
6. The production method according to claim 5, wherein, in the step (2),
relative to 10-15cm 2 The PES membrane of (1), the addition amount of procyanidin is 0.1-0.3g, and the addition amount of polyethyleneimine is 0.2-0.4g.
7. The method according to claim 1, wherein the stirring time in the step (2) is 8 to 10 hours.
8. The method according to claim 1, wherein in the step (3), the stirring time is 0 to 6 hours excluding 0.
9. The method according to claim 1, wherein in the step (3), the concentration of pepsin in the high-enzyme-activity enzyme solution is 1-6 mg/mL.
10. The modified membrane with high enzyme activity obtained by the production method according to any one of claims 1 to 9.
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| US4794002A (en) * | 1985-11-01 | 1988-12-27 | Monsanto Company | Modified polymeric surfaces and process for preparing same |
| CN1181200C (en) * | 2001-12-27 | 2004-12-22 | 复旦大学 | Method for immobilized enzyme on polymer film |
| CN1778912B (en) * | 2004-11-23 | 2010-07-07 | 孙祥章 | Method for immobilizing environmental-protecting enzyme on porous ceramic |
| CN100371372C (en) * | 2006-03-22 | 2008-02-27 | 浙江大学 | Phospholipid modified acrylon nano fiber composite film and its preparation and immobilized enzyme thereof |
| CN100534596C (en) * | 2007-08-29 | 2009-09-02 | 浙江大学 | Preparation method and application for modified acrylic fibre superfine fibre film containing reactivity group phospholipid |
| CN101775386A (en) * | 2010-01-26 | 2010-07-14 | 武汉工业学院 | Method for immobilizing trypsinase by chitosan microspheres |
| CN102660533A (en) * | 2012-05-02 | 2012-09-12 | 东华大学 | Preparation method of graphene oxide immobilized alkali protease |
| CN104548969A (en) * | 2013-10-11 | 2015-04-29 | 中国石油化工股份有限公司 | Method for preparing anti-pollution polysulfone porous membrane by self-assembling immobilization of metal ions |
| CN105316305A (en) * | 2015-11-17 | 2016-02-10 | 河南科技学院 | Method for improving pepsin activity through high pressure processing |
| CN106237869B (en) * | 2016-08-23 | 2019-05-24 | 武汉理工大学 | A kind of polyphenol coating hydrophilic method of modified hydrophobic type polymeric membrane |
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| CN108410003B (en) * | 2018-02-09 | 2020-11-06 | 江南大学 | Preparation of polyacrylonitrile modified membrane and method for applying polyacrylonitrile modified membrane to immobilized enzyme |
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