CN112480245A

CN112480245A - Application of hydrophobic cyclic peptide ligand in purification of human immunoglobulin G

Info

Publication number: CN112480245A
Application number: CN202011510975.0A
Authority: CN
Inventors: 钮雪琴
Original assignee: Individual
Current assignee: Shanghai Baijun Biotechnology Co ltd
Priority date: 2020-12-19
Filing date: 2020-12-19
Publication date: 2021-03-12
Anticipated expiration: 2040-12-19
Also published as: CN112480245B

Abstract

The invention relates to an application of a hydrophobic cyclic peptide ligand in purifying human immunoglobulin G, wherein the sequence of the hydrophobic cyclic peptide ligand is- [ HWG-C-AKTE]‑、‑[YF‑C‑WRHE]‑、‑[WV‑C‑LHHYF]‑、‑[PYF‑C‑TIE]‑、‑[FY‑C‑DEHL]The hydrophobic cyclic peptide ligand is obtained by computer simulation bionic design, and the bionic design method uses SpA and C on hIgG-Fc fragment_H3, an amino acid sequence Ser383-Asn389 on the binding region is a simulation system, a hydrophobic cyclic peptide ligand which can have a specific binding effect with the hIgG is determined through LeDock molecular docking, FlexX molecular docking and Amber molecular dynamics simulation screening, and the hIgG is purified and researched from a human serum sample by adopting the cyclic peptide ligand chromatographic medium, so that the purity of 96% and the recovery rate of 92% can be obtained.

Description

Application of hydrophobic cyclic peptide ligand in purification of human immunoglobulin G

Technical Field

The invention relates to the technical field of purification of human immunoglobulin G, and belongs to the technical field of computer simulation and downstream protein separation and purification in bionics design.

Background

Currently, antibodies are widely used in medical diagnosis and disease treatment, and more than 20 antibody drugs approved by FDA in 2006 are sold worldwide over 170 billion dollars, wherein IgG is the main antibody component in serum, accounting for about 75% of serum Ig, and is the most demanded antibody. The preparation of antibody drugs is usually completed by methods such as precipitation, ion exchange chromatography, hydrophobic interaction chromatography, affinity chromatography and the like, and the process cost accounts for about 30-40% of the production cost. Staphylococcus aureus protein A (SpA), protein G and protein L are used as affinity ligands to be widely used for preparing high-purity antibodies, the ligands have high specificity, however, too high affinity requires harsh elution conditions, target protein denaturation and ligand shedding are easily caused, adsorption capacity is low, in addition, the ligands are difficult to prepare and expensive, and the proteins generally lose partial activity after being immobilized, so that the application of the protein ligands is limited.

The polypeptide has more superiority as an affinity ligand, but the number of polypeptides having affinity with a target protein in nature is very limited, and the key to influencing the application of polypeptide affinity chromatography is the problem of how to select a proper polypeptide sequence as the affinity ligand and how to improve the affinity and selectivity of the polypeptide. The existing screening and designing methods are mainly divided into experimental screening and rational design, wherein the experimental screening is based on combinatorial library technology to carry out high-throughput experimental screening, and the rational design is mainly based on the structure and properties of target protein or existing ligands to design new ligands.

The issue of Tianjin university Sun book adopts 6 SpA key residues: f132, Y133, H137, E143, R146 and K154 construct a simulation system, and a polypeptide library is screened by molecular docking and molecular dynamics simulation, so that linear octapeptide molecules FYWHCLDE, FYTHCAKE and the like with the human IgG purity of more than 88% and the recovery rate of more than 65% can be obtained, although the purification effect is better, the method has a longer path from commercial application, and the simulation is carried out based on a hydrophobic interaction model between protein A and IgG, but the obtained polypeptide sequence is combined with hIgG based on electrostatic interaction, the electrostatic interaction is known in the art to be not specific adsorption, other biomass molecules are easy to adsorb, and the obtained protein has low purity and is difficult to be commercially utilized.

Disclosure of Invention

The invention is based on protein A and C on hIgG-Fc fragment_H3 binding region (hIgG-Fc-C)_H3) Selecting an amino acid sequence Ser383-Asn389 (SNGQPEN) in a binding region as a simulation object, constructing a cyclic peptide library taking Cys as a connecting residue by utilizing three-dimensional structure information of the simulation object, then sequentially carrying out molecular docking (twice semi-flexible molecular docking) on polypeptide molecules in the polypeptide library and an hIgG-Fc fragment by adopting a semi-flexible molecular docking program LeDock and FlexX, and selecting a polypeptide with higher binding free energy according to a scoring fractionAnd (2) performing molecular dynamics simulation between the preliminarily screened polypeptide series and the Fc fragment by using an Amber software program, excluding polypeptide sequences which cannot be stably combined with the Fc fragment, wherein the polypeptide sequences belong to a computer simulation design part of the invention, and performing an experimental verification part, namely verifying the actual combination condition and the purification effect between the simulated polypeptide sequences and the hIgG through an affinity chromatography test, so as to determine the affinity cyclic peptide ligand which can be actually combined with the hIgG in a specific manner.

The invention relates to an application of a hydrophobic cyclic peptide ligand in purifying human immunoglobulin G, wherein the sequence of the hydrophobic cyclic peptide ligand is- [ HWG-C-AKTE ] -, - [ YF-C-WRHE ] -, - [ WV-C-LHHYF ] -, - [ PYF-C-TIE ] -, - [ FY-C-DEHL ] -, and the sequence of the cyclic peptide ligand with the best purification effect is- [ HWG-C-AKTE ] -.

The hydrophobic cyclic peptide ligand is obtained by simulating a bionic design by a computer, and the bionic design method comprises the following steps: 1) constructing a simulation system: selecting the crystal conformation of protein A (SpA) -human IgG (hIgG) compound with PBD database ID of 1FC2, and extracting C on protein A and hIgG-Fc fragment_H3, a binding region, wherein the amino acid sequence of the binding region is Ser383-Asn389 (SNGQPEN), and the three-dimensional coordinates of the binding region are selected as coordinate information of a simulation system; 2) obtaining a bionic polypeptide library: calculating the distance between each residue in Ser383-Asn389 sequence by NAMD software, using Cys as connecting residue, inserting 5-7 amino acid residues X around Cys to form cyclic polypeptide chain- [ XXX-C-XXXXXX]-、-[XX-C-XXXX]-、-[XX-C-XXXXX]-、-[XXX-C-XXX]-、-[XX-C-XXX]Adopting an AutoDock molecular docking program to perform amino acid positioning, screening and determining the type of an amino acid residue X, wherein X represents a common amino acid residue in 19 except Cys, and calling a GROMOS program to obtain a biomimetic polypeptide library containing cyclic 6 peptide, cyclic 7 peptide and cyclic 8 peptide sequences; 3) preliminary screening of polypeptide library: carrying out semi-flexible docking on a polypeptide sequence in a polypeptide library and an hIgG-Fc fragment by utilizing LeDock molecular docking software; 4) re-screening of polypeptide library: performing semi-flexible molecular docking on the polypeptide sequence obtained by primary screening in the step (3) and the Fc fragment by using FlexX software; 5) molecular dynamics simulation screening: using AmbePerforming molecular dynamics simulation on the polypeptide series obtained in the step (4) by using a software package, and excluding polypeptide sequences which cannot be stably combined with the Fc fragment; 6) and (5) loading the polypeptide sequence obtained in the step (5) into a column, performing affinity chromatography purification hIgG research, calculating the purity and recovery rate of the purified hIgG, and determining effective affinity peptide ligand capable of performing stable hydrophobic binding with the hIgG.

Preferably, in the step (3), selecting polypeptide molecules with binding free energy lower than-6.0 kcal/mol according to the docking scoring score to carry out the next round of screening; and (4) selecting polypeptide molecules with the binding free energy lower than-7.0 kcal/mol according to the score for the next round of screening.

Step (2) obtaining a peptide library containing 4256 peptide library sequences in total, and selecting polypeptide molecules (1135 in total) with affinity binding force lower than-6.0 kcal/mol for the next round of screening in step (3) according to the docking scoring fraction; selecting polypeptide molecules (157 pieces in total) with affinity binding force lower than-7.0 kcal/mol according to the scoring in the step (4) for next round of screening, screening 10 polypeptide sequences which can have stable running tracks with hIgG-Fc through MD simulation, and finally screening effective affinity peptide ligands which can be specifically bound with hIgG through affinity chromatography test: the sequences are- [ HWG-C-AKTE ] - (abbreviated as pep1), [ FY-C-WRHE ] - (abbreviated as pep2), [ WV-C-LHHYF ] - (abbreviated as pep3), [ PYF-C-TIE ] - (abbreviated as pep4), [ FY-C-DEHL ] - (abbreviated as pep 5).

The above 5 affinity peptide ligands have the following simplified structural formulas:

cys residues are chosen in order to facilitate the immobilization of the polypeptide sequence on a chromatographic medium (Thiopropyl Sepharose 6B), and the structure of the peptide ligands, in which the solid spheres represent the chromatographic medium and the bonds between the solid spheres and Cys represent the disulfide bonds. After the polypeptide is connected to Thiopropyl Sepharose 6B medium, the chromatographic medium can be filled into a column for carrying out affinity chromatography purification experiment.

The present invention differs from the ligand design method and result adopted by the subject group of Sun-Yan professor of Tianjin university as follows:

1) firstly, the simulation system of the invention is different, and the invention selects protein A and C on hIgG-Fc fragment_H3-Ser 383-Asn389 (SNGQPEN) three-dimensional sequence in the binding region, rather than 6 key residues on SpA (F132, Y133, H137, E143, R146 and K154) as the mock subject, i.e. the three-dimensional structure of the template protein employed for docking is fundamentally different.

2) The LeDock molecular docking software is particularly suitable for docking of a protein ligand system, ions, metals, water molecules and the like in proteins can be removed by applying charmm force fields, the situation of LeDock on docking conformation score is superior to that of AutoDock Vina, the optimized conformation of the ligand is used as the input of molecular docking, the LeDock performance (57.4%) is superior to that of AutoDock Vina (49.0%) according to the structure of the conformation with the highest score, namely, LeDock is more suitable for docking of hIgG and a peptide ligand system, a sampling algorithm can better perform performance evaluation on the ligand in a database, and the sampling algorithm and a scoring function are the most critical calculation modules of the docking software and have decisive influence on the accuracy of a docking result.

3) The invention relates to a polypeptide library rescreening adopting FlexX as semi-flexible docking software, wherein the conformation of a peptide ligand can be changed in a certain range in the docking process by adopting the FlexX, but the change adjustment of the conformation is limited to a certain degree, for example, certain non-critical bond lengths, bond angles and the like, the conformation of an hIgG-Fc fragment is fixed, the FlexX docking method gives consideration to the calculation efficiency and precision, Flexpepdock is flexible docking, the conformations of the ligand and a template protein can be changed in the docking process, the flexible docking can accurately determine the identification condition among molecules, but the change of the conformation of the template protein and the ligand causes the calculation amount to be very large, the speed is slow, the efficiency is low, and how to accurately simulate the conformation change of a macromolecular protein is also a common difficulty of the current docking software, and a grand teaching subject group has published: the SpA-IgG binding is mainly dominated by hydrophobic interaction and hydrogen bonding, and the polypeptide is designed according to SpA-IgG complex, but the subject group of polypeptides and IgG binding is dominated by electrostatic interaction and hydrogen bonding, and is largely different from protein a in terms of binding mechanism, and the analysis reason is due to the limitation of flexpeppdock docking software, and it has been reported that molecular docking tends to underestimate hydrophobic interaction between the polypeptide and the target protein, for example, pi-pi interaction between aromatic groups, and when calculated using Flexpepdock software, the default is 0. Therefore, based on double consideration of the docking efficiency and precision, the invention adopts FlexX semi-flexible docking software instead of Flexpepdock fully flexible docking, and FlexX adopts an empirical scoring function and comprehensively considers hydrophobic interaction, electrostatic interaction and hydrogen bonds as well as pairwise preference and geometric complementarity between residues.

4) The Molecular Dynamics (MD) simulation adopts the program of AMBER instead of GROMOS, the MD simulation can simulate the action mechanism between molecules at the atomic level, and various properties of a simulation system, such as molecular conformation, energy, kinetic property, protein-ligand interaction energy and the like, can be obtained by simulating the running track of a protein-ligand system through the MD, the AMBER simulation method is adopted in the invention and is based on the method for processing cyclic peptide which is simpler and more accurate than the GROMOS, the simulation of the cyclic peptide is different from the linear peptide in that the cyclic peptide is in a way of ending and connecting with each other, the cyclic peptide needs to be processed when being read in the AMBER or the GROMOS, otherwise, the cyclic peptide is automatically processed as N-segment and C-end residues, and the AMBER processing mode is to treat two end amino acids in a pdb file of the polypeptide as common middle-end amino acid residues, so that each amino acid in a ring has no specificity, the GROMOS treatment mode of the end group amino acid is the same as the mode of defining and treating amino and carboxyl in a force field, and the cyclic peptide residue is easily taken as an N section residue and a C end residue, needs additional hydrogenation treatment, and has complex process and limited precision.

5) Compared with linear peptide molecules, the cyclic peptide ligand prepared by the invention has low flexibility, causes less entropy loss when being combined with target protein, can obtain higher binding affinity, also causes the specific conformation of lockable target molecules due to the lower flexibility, and increases the binding specificity compared with the linear peptide.

6) The affinity interaction between the cyclopeptide ligand and hIgG is mainly hydrophobic interaction, and then electrostatic interaction and hydrogen bond. The invention is based on the hydrophobic interaction model between protein A and IgG to simulate, correspondingly obtains affinity peptide ligand taking hydrophobic interaction as the leading factor, further verifies the validity of molecular simulation design, and the linear polypeptide sequence obtained by the subject group of the Sun professor based on the protein A model is combined with hIgG based on electrostatic interaction, however, the electrostatic interaction is not specific adsorption, other biomass molecules such as albumin in serum are easy to adsorb, and the purity of the obtained hIgG is difficult to be commercially utilized.

Drawings

FIG. 1 is a graph of affinity chromatography adsorption experiments on hIgG with pep1 medium, under the following conditions: adsorbing with buffer solution (20 mmol/L PBS, pH7.4), eluting with 2% acetic acid water solution;

FIG. 2 is a graph of affinity chromatography adsorption experiments on hIgG with pep2 medium, under the following conditions: adsorbing with buffer solution (20 mmol/L PBS, pH7.4), eluting with 2% acetic acid water solution;

FIG. 3 is a graph of affinity chromatography adsorption experiments on hIgG with pep3 medium, under the following conditions: adsorbing with buffer solution (20 mmol/L PBS, pH7.4), eluting with 2% acetic acid water solution;

FIG. 4 is a graph of affinity chromatography adsorption experiments on hIgG with pep4 medium, under the following conditions: adsorbing with buffer solution (20 mmol/L PBS, pH7.4), eluting with 2% acetic acid water solution;

FIG. 5 is a graph of affinity chromatography adsorption experiments on hIgG with pep5 medium, under the following conditions: adsorbing with buffer solution (20 mmol/L PBS, pH7.4), eluting with 2% acetic acid water solution;

FIG. 6 is a graph of affinity chromatography adsorption experiments on hIgG with pep6 medium, under the following conditions: adsorbing with buffer solution (20 mmol/L PBS, pH7.4), eluting with 2% acetic acid water solution;

FIG. 7 is a graph of affinity chromatography adsorption experiments on hIgG with pep1 medium, under the following conditions: adsorbing with buffer solution (20 mmol/L PBS, pH7.4), eluting with 0.5mol/L NaCl aqueous solution and 2% acetic acid aqueous solution;

FIG. 8 is a graph of an affinity chromatography purification experiment for hIgG from human serum using pep1 affinity chromatography media;

FIG. 9 is a gel electrophoresis of the purification of hIgG from human serum using pep1 affinity chromatography media.

Detailed Description

The invention will now be described in further detail with reference to the accompanying drawings, which are given by way of illustration and are not to be construed as limiting the invention in any way.

Example 1 construction of a simulation System

Selecting the crystal conformation of the SpA-hIgG1 compound with PBD database ID of 1FC2, and extracting C on SpA and hIgG-Fc fragments_H3 a binding region having the amino acid sequence Ser383-Asn389 (SNGQPEN), and the three-dimensional coordinates of each amino acid residue of the binding region are selected as coordinate information of a mimetic system.

Example 2 obtaining a library of biomimetic polypeptides

Calculating the distance between each residue in a Ser383-Asn389 sequence by using NAMD software, taking Cys as a connecting residue, inserting 5 to 7 amino acid residues X around the Cys to form a cyclic polytitanium chain- [ XXX-C-XXXXXX ] -, - [ XX-C-XXXX ] -, - [ XX-C-XXXXXX ] -, - [ XX-C-XXX ] -, performing amino acid positioning by using an AutoDock molecular docking program, determining the type of the amino acid residue X which is matched with the action of a Ser383-Asn389 coordinate system and has the best binding position, wherein X represents 19 common amino acid residues except Cys, calling a GROMOS program to obtain a biomimetic polypeptide library of a cyclic 6 peptide, a cyclic 7 peptide and a cyclic 8 peptide sequence which can be stably bound with hIgG-Fc and are connected end to end, the polypeptide library comprises 4256 sequences in total.

EXAMPLE 3 preliminary screening of polypeptide libraries

Polypeptide molecules in a polypeptide library are sequentially subjected to molecular docking with a hIgG-Fc three-dimensional structure by LeDock molecular docking software, a docking result shows that most of the polypeptides can be combined with an Fc fragment, the binding affinity is distributed between-3.5-7.6 kcal/mol, the magnitude of the affinity and the screening efficiency are comprehensively considered, and finally polypeptide molecules (1135 strips) with the binding affinity lower than-6.0 kcal/mol (the absolute value of the affinity is greater than 6.0 kcal/mol) are selected for next round of screening.

EXAMPLE 4 rescreening of polypeptide libraries

And (3) respectively carrying out semi-flexible molecular docking on the 1135 polypeptide sequences obtained by primary screening in the step (3) and hIgG-Fc again by using FlexX software, wherein the docking result shows that all the polypeptides can be combined with the Fc fragment, the combination affinity is distributed between-5.8 and-8.5 kcal/mol, and finally selecting polypeptide molecules (157 in total) with the combination affinity lower than-7.0 kcal/mol (the absolute value of the affinity is greater than 7.0 kcal/mol) for carrying out next round of screening. In the docking, the binding conformation and affinity between the ligand and the protein are screened again by using FlexX, false positive affinity ligand displayed by LeDock molecular docking is eliminated, and the accuracy is further enhanced.

Example 5 molecular dynamics simulation screening

The molecular docking is to simulate the static binding conformation between the polypeptide and the protein, and in order to further verify the affinity binding force between the pep-hIgG, the dynamic binding behavior between the polypeptide and the protein is also considered, so that the dynamic binding information between the polypeptide and the protein needs to be researched by adopting molecular dynamics simulation. And (3) performing molecular dynamics simulation on the 157 polypeptide sequences obtained in the step (4) by using an Amber software package program, wherein the dynamic process of Amber operation is divided into three steps: energy minimization, system balancing and actual kinetic simulation. Where the energy minimization is divided into two steps, the first step is mini 1: protein restriction, minimization of solvent fraction energy, second mini 2: relaxin, overall system energy minimization; the system balance is also divided into two steps: the method comprises the steps of firstly, heating a system under the condition of protein constraint, running 20ps dynamics from 0K to 300K, boosting the system, and running 100ps constant-temperature constant-pressure dynamics calculation for balancing the system; and thirdly, actually simulating, acquiring a track, and running a 10ns molecular dynamics simulation. In the simulation process, partial polypeptide molecules are gradually separated in the contact process with the Fc fragment (10 ns), namely the polypeptide molecules cannot generate stable binding effect with the Fc fragment, and finally 10 polypeptide sequences which can stably run with the hIgG-Fc are screened: - [ HWG-C-AKTE ] - (pep1), - [ FY-C-WRHE ] - (pep2), - [ WV-C-LHHYF ] - (pep3), - [ PYF-C-TIE ] - (pep4), - [ FY-C-DEHL ] - (pep5), - [ MEY-C-KAGE ] - (pep6), - [ VY-C-LEIT ] - (pep7), - [ FD-C-TPA ] - (pep8), - [ PHR-C-GAV ] - (pep9), - [ FW-C-STPR ] - (pep 10). Control experiments used pep6 peptide ligand.

Test section

Main raw materials and equipment: polypeptide molecules, gill biochemical (shanghai) ltd, synthesized by solid phase synthesis; thiopropyl Sepharose 6B medium, GE healthcare; crosslinking the cyclic peptide molecules to Thiopropyl Sepharose 6B medium to prepare polypeptide ligand medium (density: 10. mu. mol/g); hIgG, Sigma; human serum, beijing dingguo biotechnology ltd; AKTA Purifier 10 chromatograph, GE healthcare; tricorn type column, HR5/5, GE healthcare.

Example 6

Peptide ligand media (pep 1-Sepharose, pep 2-Sepharose, pep 3-Sepharose, pep 4-Sepharose, pep 5-Sepharose, pep 6-Sepharose) were loaded into a chromatography column, before loading, sufficient equilibration was performed with adsorption buffer (20 mmol/L PBS, pH 7.4), after UV baseline was stabilized, 100. mu.L of hIgG (1.0 mg/mL) dissolved in adsorption buffer was pulsed into the column at a flow rate of 50. mu.L/min, after protein injection, adsorption buffer was continuously injected into the column, unadsorbed protein was washed, then adsorbed protein was eluted with 2% aqueous acetic acid at a flow rate of 0.8 mL/min, and the eluate was tested for its absorbance at 280nm with a UV-900 detector. pep1-Sepharose chromatography experiments were additionally tested eluting with 0.5 mol/LNaCl. The chromatographic test results are shown in FIGS. 1-7.

As can be seen from the chromatograms, only trace amount of protein in the effluent components of FIGS. 1-5 flows out, which shows that hIgG can be completely combined with pep 1-pep 5 affinity media under the adsorption condition of pH7.4, the combined protein can be washed out by 2% acetic acid aqueous solution, FIG. 7 shows that NaCl solution can not elute the adsorbed protein, which shows that the combination between the peptide ligand and the hIgG is dominated by hydrophobic effect and is the same as the combination mechanism of the protein A-hIgG, which also directly proves the accuracy of the molecular simulation design of the affinity peptide ligand of the present invention. In FIG. 6, a large amount of protein flowed out directly after sample injection, and only a small amount of protein was present in the eluted fraction, indicating that pep6 could not bind effectively to hIgG and could not act as an affinity peptide ligand for hIgG.

Example 7 static adsorption isotherm determination

Fully pre-balancing a drained wet medium (pep-Sepharose 6B medium) with a buffer solution, weighing 10mg of the drained medium, mixing with a 1.0mL of an hIgG solution (dissolved by an adsorption buffer solution, 0.1-5 mg/mL), reacting for 5h at 150rpm, centrifugally separating an adsorption system at 2000rpm for 6min, collecting a supernatant, and measuring the absorbance value of the supernatant at 280nm by using a spectrophotometer to determine the protein concentration (the concentration of the protein is measured by using a spectrophotometerc) Protein adsorption Density: (q) By mass balance calculation, the Langmuir model was appliedq=q _m c/(K _d+c) Describe the adsorption isotherm, whereinq _mIs the adsorption capacity (mg-protein/g-wet medium dry-out),K _dis the apparent dissociation constant. The results are shown in Table 1.

TABLE 1

As can be seen from Table 1, the adsorption capacity of pep6 medium for hIgG was very low, only 3.8 mg/g, and again it was demonstrated that it is not an effective affinity peptide ligand for hIgG, which is in concert with the results of the affinity chromatography assay in example 5.

The adsorption capacity of pep 1-pep 5 affinity media is 91.1-136.4 mg/g, the adsorption capacity (91.1 mg/g) of pep4 is higher than FYCHWALE and FYCHTIDE, the adsorption capacity of the rest of affinity peptide ligands to hIgG is higher than the optimal peptide ligand FYWHCLDE (100.5 mg/g) obtained by the subject group of the grand professor, and the higher the adsorption capacity is, the stronger the adsorption capacity of the ligand to protein is.K _dWhich represents the dissociation constant of the liquid crystal,K _dthe smaller, indicating the higher affinity between the peptide ligand and the protein, the highest affinity was seen for pep1 and pep2, and better than FYWHCLDE, pep3-pep5 than FYCHWALE and FYCHTIDE. From the above data, it can be seen that the binding affinity of pep1 to pep5 affinity peptide ligands for hIgG is superior overall to that of the three linear octapeptide ligands of the topic group of grand professor.

Example 8 affinity chromatography purification of hIgG in human serum

Determination of the optimal affinity peptide ligand pep1 according to the test results of examples 5-6, purification of human IgG in serum using HR5/5 column containing 1mL of pep1 affinity chromatography medium, dilution (10-fold dilution) of serum sample with adsorption buffer (20 mmol/L PBS, pH 7.4), membrane filtration of diluted serum before loading to remove insoluble matter, equilibration of column with loading buffer until UV base line is leveled, then injection of 500. mu.L of human serum dilution (total protein concentration 8.86 mg/mL) at flow rate of 0.5mL/min, washing of column with loading buffer, elution of adsorbed protein with 2% aqueous acetic acid, collection of run-off fractions (FT-1, FT-2) and elution fractions (E-1, E-2), SDS-PAGE, the recovery rate of the protein was determined by using Micro-BCA kit, and the purity of the protein was analyzed by using Gel-Pro Analyzer software. The test results are shown in fig. 8 and 9.

As can be seen from the electropherograms, the effluent fraction contained a large amount of human serum albumin, only traces of human IgG and a small amount of other impurities, hIgG could be completely eluted with aqueous acetic acid (2%), and the eluted fractions E-1 and E-2 were mixed, and the purity (96%) and recovery (92%) of hIgG were calculated to be higher than those (90%) and recovery (87%) of hIgG purified from human serum by FYWHCLDE affinity media.

In summary, the experimental characterization results show that the purification effect of the designed cyclopeptide ligand on human IgG is superior to that of FYWHCLDE, FYCHWALE and FYCHTIDE peptide ligand media on the whole in the aspects of adsorption capacity, binding affinity, binding purification and recovery rate and the like.

That is, compared with the rational design method proposed by the group of subjects taught in the grand professor, the peptide ligands of the present invention are designed by molecular simulation using a simulation system, molecular docking software, molecular dynamics simulation program, and simulation parameters different from those of the subject group, and the peptide ligands thus selected are different in shape, action on hIgG (apparently, different buffers used for adsorption and elution), and binding force and affinity to hIgG. Therefore, the two ligands are not comparable, and the purification effect of the cyclic peptide ligands of the present invention is superior to the ligands obtained in the subject group as a whole.

Claims

1. Use of a hydrophobic cyclic peptide ligand for purification of human immunoglobulin G, characterized in that: the sequence of the hydrophobic cyclic peptide ligand is- [ HWG-C-AKTE ] -, - [ YF-C-WRHE ] -, - [ WV-C-LHHYF ] -, - [ PYF-C-TIE ] -, - [ FY-C-DEHL ] -.

2. Use of a hydrophobic cyclic peptide ligand according to claim 1 for the purification of human immunoglobulin G, wherein: the sequence of the hydrophobic cyclic peptide ligand is preferably- [ HWG-C-AKTE ] -.

3. Use of a hydrophobic cyclic peptide ligand according to any one of claims 1-2 for the purification of human immunoglobulin G, wherein: the hydrophobic cyclic peptide ligand is obtained by computer simulation bionic design.

4. Use of a hydrophobic cyclic peptide ligand according to claim 3 for the purification of human immunoglobulin G, wherein: the bionic design method comprises the following steps:

(1) constructing a simulation system: selecting the crystal conformation of protein A (SpA) -human IgG (hIgG) compound with PBD database ID of 1FC2, and extracting C on protein A and hIgG-Fc fragment_H3, a binding region, wherein the amino acid sequence of the binding region is Ser383-Asn389 (SNGQPEN), and the three-dimensional coordinates of the binding region are selected as coordinate information of a simulation system;

(2) obtaining a bionic polypeptide library: calculating the distance between each residue in a Ser383-Asn389 sequence by using NAMD software, taking Cys as a connecting residue, inserting 5 to 7 amino acid residues X around the Cys to form a cyclic polypeptide chain- [ XXX-C-XXXXX ] -, - [ XX-C-XXX ] -, performing amino acid positioning by using an AutoDock molecular docking program, screening to determine the type of the amino acid residue X, wherein X represents a common amino acid residue in 19 except Cys, and calling a GROMOS program to obtain a biomimetic polypeptide library comprising cyclic 6 peptide, cyclic 7 peptide and cyclic 8 peptide sequences;

(3) preliminary screening of polypeptide library: carrying out semi-flexible docking on a polypeptide sequence in a polypeptide library and an hIgG-Fc fragment by utilizing LeDock molecular docking software;

(4) re-screening of polypeptide library: performing semi-flexible molecular docking on the polypeptide sequence obtained by primary screening in the step (3) and the Fc fragment by using FlexX software;

(5) molecular dynamics simulation screening: performing molecular dynamics simulation on the polypeptide series obtained in the step (4) by using an Amber software package, and excluding polypeptide sequences which cannot be stably combined with the Fc fragment;

(6) and (5) loading the polypeptide sequence obtained in the step (5) into a column, performing affinity chromatography purification hIgG research, calculating the purity and recovery rate of the purified hIgG, and determining effective affinity peptide ligand capable of performing stable hydrophobic binding with the hIgG.

5. Use of the hydrophobic cyclic peptide ligands according to claim 3 for purifying human IgG, wherein in step (3) the polypeptide molecules with binding free energy lower than-6.0 kcal/mol are selected for the next round of screening according to the docking scoring score.

6. Use of the hydrophobic cyclic peptide ligands according to claim 3, wherein the polypeptide molecules with binding free energy lower than-7.0 kcal/mol are selected according to the score in step (4) for the next round of screening.