CN112451158A

CN112451158A - Cataract detection device for mice

Info

Publication number: CN112451158A
Application number: CN202011408526.5A
Authority: CN
Inventors: 李崇义; 耿钊; 刘宇辉; 熊艳丽; 张洁琼; 叶剑; 刘莛; 刘玮
Original assignee: Chinese Peoples Liberation Army Army Specialized Medical Center
Current assignee: Chinese Peoples Liberation Army Army Specialized Medical Center
Priority date: 2020-12-04
Filing date: 2020-12-04
Publication date: 2021-03-09
Anticipated expiration: 2040-12-04
Also published as: CN112451158B

Abstract

The invention discloses a mouse cataract detection device, which comprises a workbench and a mouse fixing device arranged on the workbench, and also comprises: the electron microscope is arranged on the workbench in an adjustable position so as to obtain the amplified visual field of the eyes of the mice; the display is electrically connected with the electron microscope to display the amplified image of the eyes of the white mouse; the eyelid support ring comprises an elastic ring body and two clamping bodies which are symmetrically arranged on the ring body and extend outwards in parallel with the central axis of the ring body, and the ring body is in a circular shape with the diameter of 3-7mm in a natural relaxation state under the self elasticity; and the upper ring pliers are elbow pliers with the pliers heads and the pliers handles forming an included angle, and the end parts of the pliers heads of the upper ring pliers are provided with hole-shaped structures movably matched with the clamping bodies of the eyelid support rings. By adopting the mouse cataract detection device, the light, safe and accurate mouse eye cataract detection experiment can be ensured.

Description

Cataract detection device for mice

Technical Field

The invention relates to the technical field of mouse experimental models, in particular to a mouse cataract detection device.

Background

Cataract is the eye pathological change which is ranked at the head of pathogenic eye diseases worldwide at present, and the detection research, prevention and treatment of cataract naturally become the key point of ophthalmological scientific research. The establishment of the cataract animal model by taking the mouse as an experimental object is an effective and practical means for researching the pathogenesis and exploring the treatment method.

However, the eyeball of the white mouse is different from human eyes and other larger mammals, the diameter of the eyeball is only 3-7mm, the eyeball is convex, and further, in the experimental research process of carrying out cataract detection on the eyeball of the white mouse, the eyelid is difficult to be opened by using a conventional eyelid retractor as an eyelid opener, the visual field of the eyeball is exposed, and the eyelid can be opened only by using a traditional manual mode of eyelid opening by using tweezers in most cases.

However, when the eye cataract of the mouse is detected by the forceps through the eyelid opening, the eye cataract detection can be performed only by assistance of an assistant, the size and the direction of the eyelid opening are difficult to control stably, the long-time stable positioning control is difficult, the operation difficulty is high, pressure is easy to be generated on eyeballs, and the detection result is influenced.

Disclosure of Invention

Based on the above, the invention aims to provide a mouse cataract detection device to solve the problems of high eyelid opening operation difficulty and inaccurate detection result in the existing mouse cataract detection experiment.

In order to solve the technical problems, the invention adopts the technical scheme that:

mouse cataract detection device, including workstation and the white mouse fixing device of setting on the workstation, still include:

the electron microscope is arranged on the workbench in an adjustable position so as to obtain the amplified visual field of the eyes of the mice;

the display is electrically connected with the electron microscope to display the amplified image of the eyes of the white mouse;

the eyelid support ring comprises an elastic ring body and two clamping bodies which are symmetrically arranged on the ring body and extend outwards in parallel with the central axis of the ring body, and the ring body is in a circular shape with the diameter of 3-7mm in a natural relaxation state under the self elasticity; and

the upper ring clamp is an elbow clamp with an included angle formed between a clamp head and a clamp handle, and a hole-shaped structure movably matched with the clamping body of the eyelid support ring is arranged at the clamp head end of the upper ring clamp.

Preferably, the radial section of the ring body is crescent-shaped, and the crescent-shaped convex surface is positioned on the inner ring surface of the ring body.

Preferably, the cataract detection device for mice further comprises: an eyelid opening device, the eyelid opening device comprising:

the fixing seat can be stuck and fixed on the eye periphery of the mouse and is provided with a viewing window for exposing the eye part of the mouse, and the inner surfaces of the two sides of the upper eyelid and the lower eyelid of the fixing seat are concavely provided with sliding grooves communicated with the viewing window;

the eyelid patch is respectively arranged in the viewport corresponding to the upper eyelid and the lower eyelid of the white mouse in two pieces, the surface of the eyelid patch facing the eyelid is provided with viscose, and the two eyelid patches can slide in the sliding groove in the opposite direction or in the opposite direction;

the sliding rods are respectively arranged in the fixed seats at the two sides of the viewing window along the opening and closing direction of the eyelids of the white mouse, the sliding rods are in sliding fit with the fixed seats, and the close ends of the two sliding rods are respectively connected with the eyelid patches at the corresponding side; and

and the elastic piece is arranged in the fixed seat and provides elastic force for the two sliding rods to be away from each other.

Preferably, the eyelid opening device further comprises: the fixing patch is detachably attached to the face, back to the eyelid of the white mouse, of the eyelid patch and is connected with the two eyelid patches to enable the similar edges of the two eyelid patches to be close to and spliced.

Preferably, the cataract detection device for mice further comprises: an adjusting bracket; the adjusting bracket includes: stand and horizontal pole, the stand sets up on the workstation in the white mouse fixing device outside, the one end of horizontal pole is connected on the stand and can be fixed to the stand on arbitrary position, the other end of horizontal pole is provided with and is used for fixing electron microscope's fixture.

Preferably, the white rat fixing device comprises: the mouse fixing device comprises a sliding table plate and fixing belts, wherein the sliding table plate is arranged on the upper surface of a workbench in a sliding mode, the fixing belts are arranged on the surfaces, located on the two sides of the sliding direction of the sliding table plate, of the workbench, and the fixing belts can be used for fixing the sliding table plate and a mouse placed on the fixing plates at the same time.

Preferably, the cataract detection device for mice further comprises: the buffering support mechanism comprises a guide rod and a tension spring, one end of the guide rod is connected with the cross rod in a spherical hinge mode, and the other end of the guide rod can extend towards the white rat fixing device; the upper ring pliers are arranged on the guide rod and can slide along the guide rod; one end of the tension spring is connected with the cross rod, and the other end of the tension spring is connected with the ring fitting pliers.

Preferably, the hole-shaped structure is a sleeve, the two sleeves are respectively arranged on the close side surfaces of the two forceps heads of the upper ring forceps, and the pipe hole of each sleeve is movably matched with the clamping body.

Preferably, the hole structure comprises: and the lantern rings are respectively arranged at the end parts of the two forceps heads of the upper ring forceps, and the annular holes of the lantern rings are movably matched with the clamping bodies.

Preferably, the hole structure further comprises: correspond every the lantern ring sets up the supporter on the binding clip above the lantern ring, the supporter is the arc coaxial with the homonymy lantern ring, and two the supporter sets up the lateral part close at two binding clips respectively.

Compared with the prior art, the mouse cataract detection device can clearly display the eye and eye periphery states of the mouse on the workbench through the amplification display system consisting of the electron microscope and the display, so that the experimental operations such as eyelid opening, detection and the like can be performed around the eyes of the mouse; the eyelid opening operation is completed by matching the specially designed upper ring clamp with the eyelid supporting ring, the hole-shaped structure of the upper ring clamp is inserted into the clamping body of the eyelid supporting ring, an operator clamps the clamp handle to pick up the eyelid supporting ring, and simultaneously the ring body of the eyelid supporting ring is elastically deformed into an elliptical shape under the action of clamping force, so that the eyelid of a white mouse can be opened by the operator through the thumb and the forefinger of one hand, the flattened ring body can be placed into the eye socket of the white mouse by the other hand, the upper ring clamp is released afterwards, the ring body recovers a circular shape under the action of self elasticity to support the eyelid, and the long-time support of the eyelid can be realized by removing the upper ring clamp at last, so that the eye condition of the white mouse can be clearly observed through the electron microscope, and the experimental operation can be accurately and reliably completed. Whole experimentation just can operate the completion alone, and the operation degree of difficulty is little, and is less to the oppression of mice eye, can be long-time stable detect, and observe the field of vision clear, guarantees the accuracy of experimental result.

Drawings

In order to more clearly illustrate the embodiments of the present invention, the drawings, which are required to be used in the embodiments, will be briefly described below. In all the drawings, the elements or parts are not necessarily drawn to actual scale.

FIG. 1 is a schematic perspective view of an embodiment of the present invention;

FIG. 2 is a schematic cross-sectional view of an eyelid support ring in accordance with one embodiment of the present invention;

FIG. 3 is a schematic view of the engagement of upper ring clamps with an eyelid support ring in accordance with one embodiment of the present invention;

figure 4 is a schematic perspective view of an embodiment of the eyelid opening device of the present invention;

figure 5 is a side view of the eyelid opening device in accordance with one embodiment of the present invention;

FIG. 6 is a schematic cross-sectional view of the end face A-A of FIG. 5;

FIG. 7 is a schematic view of a usage status of an embodiment of the present invention.

Reference numerals:

1-a workbench and 11-a chute;

2-a white mouse fixing device, 21-a slide table plate and 22-a fixing belt;

3-electron microscope;

4-a display;

5-eyelid support ring, 51-ring, 52-clamping body;

6-upper ring clamp, 61-clamp head, 62-clamp handle, 63-hole structure, 631-lantern ring, 632-support body;

7-eyelid opening device, 71-fixing seat, 72-viewing window, 73-sliding groove, 74-eyelid patch, 75-sliding rod, 76-elastic piece, 77-fixing patch;

8-adjusting frame, 81-upright post, 82-cross bar, 83-clamping mechanism;

9-a buffer supporting mechanism, 91-a guide rod and 92-a tension spring;

i-white mouse I, II-eye periphery, III-eyelid, IV-eyeball.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, but rather should be construed as broadly as the present invention is capable of modification in various respects, all without departing from the spirit and scope of the present invention.

It is to be noted that, unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

As shown in fig. 1, the main structure of the mouse cataract test device provided in this embodiment includes a workbench 1 similar to a conventional mouse experimental device and a mouse fixing device 2 disposed on the workbench 1, except that the device is provided with an electron microscope 3 and a display 4 on the workbench 1, and is also provided with a specially-made eyelid supporting ring 5 and a ring-closing clamp 6.

Referring to fig. 1, an electron microscope 3 is mounted on a worktable 1 in an adjustable position to obtain an enlarged field of vision of the eyes of a mouse I; the display 4 is electrically connected to the electron microscope 3 to display the magnified image of the eye of the mouse I. The electron microscope 3 captures images through an electron camera, is connected with the display 4 through a WIFI wireless mode, a USB data line mode and the like, and outputs the captured images to display equipment such as a television screen and the like, so that magnified images which are magnified by a plurality of times to hundreds of times are obtained, for example, an AO-AF216C high-definition automatic focusing microscope produced by Aus micro optical instrument Limited in Shenzhen, and the magnification is 10-200 times.

In order to facilitate the experiment operation and the accurate focusing of the electron microscope 3, in a specific embodiment, an adjusting bracket 8 may be additionally provided to assist in mounting the electron microscope 3. Referring to fig. 1, the adjusting bracket 8 in one embodiment includes: the device comprises a vertical column 81 and a cross rod 82, wherein the vertical column 81 is arranged on the workbench 1 at the outer side of the white rat fixing device 2, one end of the cross rod 82 is connected with the vertical column 81 and can be fixed to any position of the vertical column 81, and the other end of the cross rod 82 is provided with a clamping mechanism 83 for fixing the electron microscope 3. Further, the cross bar 82 can adjust the up-and-down position and the rotation angle on the column 81, thereby adjusting the position of the electron microscope 3 in two dimensions.

In the case of performing a mouse cataract test, a mouse I is fixed to the table 1 (preferably, a mouse after anesthesia) by the mouse fixing device 2, and specifically, the mouse fixing device 2 may be provided in a structure including a slide plate 21 and a fixing band 22. As shown in fig. 1, the slide plate 21 is slidably disposed on the upper surface of the workbench 1, specifically, a slide groove 11 may be disposed on the workbench 1, the slide plate 21 is mounted on the workbench 1 and realizes the position adjustment of the white rat fixing device 2 by the cooperation with the slide groove 11; the fixing belts 22 are provided on the surfaces of the table 1 on both sides in the sliding direction of the platen 21, and the fixing belts 22 can fix the platen 21 and the mouse I placed on the fixing plates at the same time. Through the sliding of the sliding table plate 21, the two-dimensional adjustment of the adjusting frame 8 is matched, so that the three-dimensional position adjustment of the electron microscope 3 is realized, and the accurate focusing can be ensured under various conditions to obtain a clear image of the eye part of the white mouse I.

After the mouse I is fixed on the workbench 1, the eyelid is also required to be opened firstly when the experiment is started, and the specific eyelid opening operation is completed by matching the specially designed upper ring forceps 6 with the eyelid supporting ring 5.

Referring to fig. 2, the eyelid supporting ring 5 includes an elastic ring body 51, and two clamping bodies 52 symmetrically disposed on the ring body 51 and extending outward parallel to the central axis of the ring body 51, and the ring body 51 is in a circular shape with a diameter of 3-7mm in a natural relaxation state under its own elastic force. Simultaneously, in order to improve the stability of the eyelid III support after the ring body 51 is placed in the orbit of the white mouse I, as shown in fig. 2 and 7, the ring body 51 can be further arranged to be of a crescent-shaped structure with a radial section, the crescent-shaped convex surface is positioned on the inner ring surface of the ring body 51, and the crescent-shaped concave arc surface contacts with the eyelid III, so that the stability of the eyelid support is simply and effectively improved, and the long-time, stable and clear experiment can be more favorably completed.

Referring to fig. 1 to 3, the upper ring clamp 6 is an elbow clamp with an included angle between the clamp head 61 and the clamp handle 62, and the end of the clamp head 61 of the upper ring clamp 6 is provided with a hole-shaped structure 63 movably matched with the clamping body 52 of the eyelid supporting ring 5. The hole-shaped structure 63 may be a sleeve, the two sleeves are respectively disposed on the adjacent side surfaces of the two bits 61 of the upper ring clamp 6, and the tube hole of the sleeve is movably adapted to the clamping body 52. Thus, the two holders 52 of the eyelid supporting ring 5 are fitted with the sleeve, the eyelid supporting ring 5 can be picked up by applying the lateral pressure F to the upper ring gripper 6 by the gripper 62, and the lateral pressure F applied between the holders 52 and the sleeve can be removed smoothly by releasing the gripper 62.

Referring to fig. 3, in consideration of the fact that the sleeve has a certain length and thus has a large area of matching contact with the holding body 52, there may be an accident that the eyelid supporting ring 5 is taken out due to too large friction force when separating, and the hole-shaped structure 63 in another preferred embodiment includes the ring-shaped rings 631 respectively disposed at the ends of the two bits 61 of the upper ring clamp 6, and the ring holes of the ring 631 are movably fitted with the holding body 52. In this way, the friction factor when separating the upper ring clamp 6 from the eyelid supporting ring 5 is substantially eliminated by the slight point-line contact between the collar 631 and the clamping body 52. Of course, since the pressing area contacted by the collar 631 is small, the pressing effect of the upper ring clamp 6 on the eyelid supporting ring 5 is also weakened, the requirement for skill and proficiency of clamping the eyelid supporting ring 5 by the upper ring clamp 6 is relatively high, and in order to make up for the disadvantage, the support 632 may be added to the collar 631 in pair.

Referring to fig. 3 and 6, the supporting body 632 is disposed on the binding clip 61 above the collar 631 corresponding to each collar 631, the supporting body 632 is in an arc shape coaxial with the collar 631 on the same side, and the two supporting bodies 632 are disposed on the adjacent side portions of the two binding clips 61 respectively. When the collar 631 presses the root of the clamping body 52, the end of the clamping body 52 tends to tilt inward, which is stopped by the support 632, ensuring that the lateral pressure F applied by the clamp 61 to the clamping body 52 is effectively transmitted to the ring body 51, collapsing the ring body 51; meanwhile, after the lateral pressure F applied between the clamping body 52 and the sleeve is removed, the clamping body 52 and the hole-shaped structure 63 which are in two-point contact do not leave much friction force, and smooth separation of the clamping body 52 and the hole-shaped structure is further ensured.

Referring to fig. 7, the mouse cataract measurement device with the above structure can clearly display the eye and eye circumference II states of the mouse I on the worktable 1 through the magnifying display system composed of the electron microscope 3 and the display 4, so as to perform the experimental operations such as eyelid opening and measurement on the eye circumference II of the mouse I. In a specific experiment, firstly opening the eyelid, inserting the hole-shaped structure 63 of the upper ring clamp 6 into the clamping body 52 of the eyelid supporting ring 5, picking up the eyelid supporting ring 5 by the clamping clamp handle 62 of an operator, elastically deforming the ring body 51 of the eyelid supporting ring 5 into an oval shape under the action of the clamping side pressure F, further opening the eyelid III of the white mouse I by the thumb and the index finger of one hand with the aid of the electron microscope 3, putting the flattened ring body 51 into the eye socket of the white mouse I by the upper ring clamp 6 by the other hand, then loosening the eyelid III and the upper ring clamp 6, restoring the ring body 51 to be circular under the action of the self-elasticity to support the eyelid III based on the convex structure characteristic of the eyeball IV of the white mouse I and the automatic closing force after the eyelid III loses the opening force, finally removing the upper ring clamp 6 to realize long-time support of the eyelid III, and finally clearly observing the eye condition of the white mouse I by the electron microscope 3, the experimental operation is accurately and reliably completed. Whole experimentation just can operate the completion alone, and the operation degree of difficulty is little, and is less to the oppression of mice I eye, can detect for a long time stably, and observes the field of vision clear, guarantees the accuracy of experimental result.

As shown in fig. 4 to 7, in other embodiments, an eyelid opening device 7 is added to the mouse cataract measurement device described above, considering that the operator is manually operated to open the eyelid III of the mouse I with the thumb and the index finger of one hand, which still has a safety risk and certain inconvenience. The eyelid opening device 7 includes: a fixed base 71, an eyelid patch 74, a sliding rod 75, and an elastic member 76.

The fixing seat 71 can be adhered and fixed to the eye periphery II of the mouse I, and is provided with a viewing window 72 exposing the eye of the mouse I, and the inner surfaces of the fixing seat 71, which are positioned on the two sides of the upper eyelid III and the lower eyelid III of the mouse I, are concavely provided with sliding grooves 73 communicated with the viewing window 72. The eyelid patches 74 are respectively arranged in the viewing window 72 in two pieces corresponding to the upper eyelid and the lower eyelid of the white mouse I, the surface of the eyelid patches 74 facing the eyelid III is provided with adhesive, and the two eyelid patches 74 can slide in the sliding groove 73 in the opposite direction or in the opposite direction, so as to open or close the viewing window 72. The sliding rods 75 are respectively arranged in the fixing seats 71 on two sides of the viewing window 72 along the opening and closing direction of the eyelid III of the white mouse I, the sliding rods 75 are in sliding fit with the fixing seats 71, the close ends of the two sliding rods 75 are respectively connected with the eyelid patches 74 on the corresponding side, and then the sliding rods 75 can drive the eyelid patches 74 to move in a sliding mode. The elastic member 76 is disposed in the fixing base 71 to provide an elastic force for the two sliding rods 75 to move away from each other.

When the eyelid retractor 7 is used, an operator pinches the sliding rods 75 at two sides with the thumb and the forefinger of one hand to enable the two eyelid patches 74 to be in a close splicing state shown in fig. 4, then presses the eyelid patches II of the white mouse I, enables the splicing seams of the two eyelid patches 74 to be aligned with the gaps of the upper eyelid III and the lower eyelid III of the white mouse I, pressurizes the fixing seat 71 to be pasted and fixed on the skin of the eyelid circumference II of the white mouse I, lightly strops the eyelid patches 74 to enable the two eyelid patches 74 to be respectively pasted on the eyelid III at one side, and finally releases fingers, as shown in fig. 7, the sliding rods 75 slide outwards under the elastic force of the elastic piece 76 to further drive the eyelid patches 74 to pull the pasted eyelid III to two sides to expose the inner visual field of the eye socket, so that the flattened eyelid support ring 5 can be conveniently placed, and the eyelid support III effectively after the ring body 1 is restored to be circular.

Of course, in order to improve the using effect of the eyelid retractor 7, the hair around the eye of the white mouse I can be shaved first, so as to improve the adhesive fixing effect of the fixing base 71. A binding belt can be arranged on the fixed seat 71 and can be more firmly bound to the head of the white mouse I. Meanwhile, the shape of the fixed seat 71 is preferably adapted to the body shape of the mouse I around the eyes; the fixing seat 71 can also be made of plastic materials which can bend to a certain degree towards the two sides of the sliding rod 75, so that the attaching stability is ensured, and the sliding capacity of the sliding rod 75 in the fixing seat 71 is not influenced.

In order to drive the sliding rod 75 to slide in the fixing seat 71, the elastic member 76 in one embodiment is a tension spring. As shown in fig. 6, a space for accommodating a tension spring is provided on the mating surfaces of the sliding rod 75 and the fixed base 71, and both ends of the tension spring are respectively connected to an end of the fixed base 71 away from the viewing window 72 and an end of the sliding rod 75 close to the viewing window 72. Therefore, when the sliding rods 75 on the two sides are pinched for installation, the tension spring is lengthened for accumulating force, and after the sliding rods 75 are released, the tension spring is restored to pull the sliding rods 75 to move outwards, so that automatic eyelid opening is realized.

Of course, it is inconvenient to press the fixing base 71 to adhere the fixing base 71 while applying a lateral pressure to the sliding rod 75 with fingers. In yet another embodiment, a fixation patch 77 is further provided for the eyelid opening device 7.

Referring to fig. 4 and 6, the fixing patch 77 is detachably attached to a surface of the eyelid patch 74 facing away from the eyelid III of the white mouse I, and the two eyelid patches 74 are connected such that the adjacent edges of the two eyelid patches 74 are closely joined. That is, the fixed patch 77 maintains the state in which the slide rod 75 moves inward and the eyelid patch 74 is closed as shown in fig. 4 and 5, and the operator only needs to press the fixing base 71 to fix the eyelid patch, and then tears off the fixed patch 77, and the resistance for preventing the elastic member 76 from returning is automatically removed, thereby realizing more automatic eyelid opening operation.

As shown in fig. 1 and 3, the mouse cataract measurement device according to another embodiment further includes: and a buffer support mechanism 9. The buffering support mechanism 9 comprises a guide rod 91 and a tension spring 92, one end of the guide rod 91 is in spherical hinge connection with the cross rod 82, and the other end of the guide rod 91 can extend towards the white rat fixing device 2; the upper ring clamp 6 is arranged on the guide rod 91 and can slide along the guide rod 91; one end of a tension spring 92 is connected with the cross bar 82, and the other end of the tension spring 92 is connected with the upper ring clamp 6. Like this through buffering supporting mechanism 9 to fitting with a contraceptive ring pincers 6 and lead to, provide simultaneously and pull the resistance, when the operator passes through fitting with a contraceptive ring pincers 6 and installs eyelid support ring 5, can avoid the installation difficulty that the hand shake caused, also can avoid exerting oneself the shakiness and cause the injury to eyeball IV, putting into of eyelid support ring 5 is more steady and safe, improves the security of whole experiment operation.

In conclusion, the device for detecting the cataract of the mouse provided by the invention can effectively solve the problems of high eyelid opening operation difficulty and inaccurate detection result in the existing mouse cataract detection experiment, and ensures that the mouse cataract detection experiment is easily, safely and accurately performed.

The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention, and they should be construed as being included in the following claims and description.

Claims

1. The utility model provides a mouse cataract detection device, includes workstation and the mouse fixing device of setting on the workstation, its characterized in that still includes:

2. The mouse cataract detection device of claim 1, wherein the radial cross section of the ring body is crescent-shaped, and the crescent-shaped convex surface is located on the inner ring surface of the ring body.

3. The mouse cataract measurement device according to claim 1, further comprising: an eyelid opening device, the eyelid opening device comprising:

4. The mouse cataract measurement device of claim 3, wherein the eyelid opening device further comprises: the fixing patch is detachably attached to the face, back to the eyelid of the white mouse, of the eyelid patch and is connected with the two eyelid patches to enable the similar edges of the two eyelid patches to be close to and spliced.

5. The mouse cataract measurement device according to claim 1, further comprising: an adjusting bracket; the adjusting bracket includes: stand and horizontal pole, the stand sets up on the workstation in the white mouse fixing device outside, the one end of horizontal pole is connected on the stand and can be fixed to the stand on arbitrary position, the other end of horizontal pole is provided with and is used for fixing electron microscope's fixture.

6. The mouse cataract measurement device of claim 5, wherein the mouse fixing device comprises: the mouse fixing device comprises a sliding table plate and fixing belts, wherein the sliding table plate is arranged on the upper surface of a workbench in a sliding mode, the fixing belts are arranged on the surfaces, located on the two sides of the sliding direction of the sliding table plate, of the workbench, and the fixing belts can be used for fixing the sliding table plate and a mouse placed on the fixing plates at the same time.

7. The mouse cataract measurement device according to claim 5, further comprising: the buffering support mechanism comprises a guide rod and a tension spring, one end of the guide rod is connected with the cross rod in a spherical hinge mode, and the other end of the guide rod can extend towards the white rat fixing device; the upper ring pliers are arranged on the guide rod and can slide along the guide rod; one end of the tension spring is connected with the cross rod, and the other end of the tension spring is connected with the ring fitting pliers.

8. The apparatus for detecting cataract of mouse as claimed in any one of claims 1 to 7, wherein the hole-shaped structure is a sleeve, two sleeves are respectively disposed on the adjacent side surfaces of the two forceps heads of the upper ring forceps, and the tube hole of the sleeve is movably fitted with the clamping body.

9. The mouse cataract measurement device of any one of claims 1-7, wherein the aperture-like structure comprises: and the lantern rings are respectively arranged at the end parts of the two forceps heads of the upper ring forceps, and the annular holes of the lantern rings are movably matched with the clamping bodies.

10. The mouse cataract measurement device of claim 9, wherein the aperture structure further comprises: correspond every the lantern ring sets up the supporter on the binding clip above the lantern ring, the supporter is the arc coaxial with the homonymy lantern ring, and two the supporter sets up the lateral part close at two binding clips respectively.